Method for inducing cyanobacteria to produce natural sunscreen substances by ultraviolet rays and application thereof

Abstract

The present application relates to the technical field of natural sunscreen substance production technology, and provides a method for producing natural sunscreen substances by ultraviolet-induced cyanobacteria. By applying ultraviolet irradiation of specific wavelength and intensity to a cyanobacteria culture system and adding an inducer, i.e. a tetrapyrrole compound, to the culture system, the production of natural sunscreen substances by cyanobacteria can be effectively promoted. The method for producing natural sunscreen substances by ultraviolet-induced cyanobacteria and the application thereof not only provide a new and sustainable way for the production of sunscreen products, but also have good performance and safety, and have wide application prospects and important social significance in the fields of cosmetics, skincare products and medicines.

Description

Technical Field

[0001] This invention relates to the field of natural sunscreen production technology, specifically to a method for inducing cyanobacteria to produce natural sunscreen substances using ultraviolet light and its application. Background Technology

[0002] With increasing public awareness of health and beauty, sun protection has become an essential part of daily life. Ultraviolet (UV) radiation poses various health risks to the skin, such as sunburn, tanning, premature aging (wrinkles, sagging), and an increased risk of skin cancer. To combat the harmful effects of UV radiation, sunscreen products are widely used.

[0003] Traditional sunscreens are primarily based on chemically synthesized sunscreens, such as benzophenones and salicylates. However, these chemically synthesized sunscreens have some limitations. On the one hand, some chemical sunscreens may cause allergic reactions, making them unsuitable for people with sensitive skin. On the other hand, the production process of chemical sunscreens may cause some environmental pollution, and after use, they may enter the natural environment through rainwater runoff, potentially having a negative impact on ecosystems.

[0004] Natural sunscreens have advantages such as being naturally sourced, having low skin irritation, and being environmentally friendly. However, existing natural sunscreens, such as plant extracts, have limited sun protection effects, are complex to extract, and their production volume is difficult to meet demand.

[0005] Cyanobacteria, as photosynthetic microorganisms, possess mechanisms to cope with ultraviolet radiation and can synthesize natural sunscreen substances, making them highly promising producers of natural sunscreens. However, effectively inducing cyanobacteria to produce natural sunscreens in large quantities presents numerous challenges in terms of production efficiency, yield control, and product quality stability, making it difficult to meet the demands of commercial production.

[0006] Therefore, this proposal suggests a method for inducing cyanobacteria to produce natural sunscreen substances using ultraviolet light and its application, in order to solve the above problems. Summary of the Invention

[0007] To overcome the shortcomings of the prior art, the present invention aims to provide a method for inducing cyanobacteria to produce natural sunscreen substances by ultraviolet light and its application.

[0008] To achieve the aforementioned objective, the technical solution of the present invention is implemented as follows: a method for inducing cyanobacteria to produce natural sunscreen substances using ultraviolet light:

[0009] Provides cyanobacteria culture systems:

[0010] The cyanobacteria culture system comprises a specific culture medium, the components of which, per liter, are: 0.5-2.0 g sodium nitrate, 0.02-0.1 g potassium dihydrogen phosphate, 0.05-0.2 g magnesium sulfate, 0.01-0.05 g calcium chloride, 1-5 ml trace element solution, and the remainder being water; the trace element solution, per liter, comprises: 0.5-2.0 g boric acid, 0.05-0.3 g manganese chloride, 0.01-0.1 g zinc sulfate, 0.005-0.02 g sodium molybdate, 0.0005-0.002 g copper sulfate, and 0.0005-0.002 g cobalt chloride.

[0011] The cyanobacterial species is Nostoc desertis;

[0012] The cyanobacteria culture system was subjected to ultraviolet irradiation of specific wavelengths and intensities:

[0013] The wavelength of the ultraviolet light is 280-320nm;

[0014] The intensity of the ultraviolet light is 5-20 microwatts per square centimeter;

[0015] The ultraviolet irradiation is pulsed, with a pulse frequency of 1-5 Hz, a pulse duration of 1-10 seconds, and a total irradiation time of 1-6 hours.

[0016] After exposure to ultraviolet light, continue cultivating cyanobacteria to produce natural sunscreen substances:

[0017] The temperature for continued cultivation is 25℃;

[0018] The pH level for continued culturing should be 7.5-9.0;

[0019] The culture period continues for 24-72 hours.

[0020] Preferably, the cyanobacteria culture system is in the logarithmic growth phase before ultraviolet irradiation. The growth stage of the cyanobacteria is determined by measuring the optical density value (OD680) at 680 nm. When OD680 reaches 0.3-0.8, it is determined to be in the logarithmic growth phase.

[0021] During ultraviolet irradiation, the cyanobacteria culture system is stirred at a speed of 50-200 rpm.

[0022] The cyanobacteria culture system employs a photobioreactor during the cultivation process. The light intensity of the photobioreactor is 100-500 micromolar photons / m²·second, and the light-dark cycle is 12 hours:12 hours.

[0023] Preferably, the natural sunscreen substance contains mycotoxin-like amino acids (MAAs), and the content of MAAs accounts for 30%-70% of the total weight of the natural sunscreen substance;

[0024] The natural sunscreen substance absorbs ultraviolet light in the wavelength range of 280-400nm, and its ultraviolet absorption coefficient in the wavelength range of 310-360nm is 100-500L / (mol·cm).

[0025] Preferably, the sunscreen product is in the form of a lotion, and the lotion contains the following ingredients:

[0026] Natural sunscreen ingredients: 5%-20%;

[0027] Oil phase composition: 10%-30%, wherein the oil phase composition includes one or more of olive oil and squalane;

[0028] Aqueous phase composition: 40%-70%, wherein the aqueous phase composition includes one or more of deionized water, glycerin, and sodium hyaluronate;

[0029] Emulsifier: 1%-5%, wherein the emulsifier is one or more of polysorbate-80 and cetearyl alcohol polyether-20;

[0030] Preservative: 0.1%-1%, wherein the preservative is one or more of phenoxyethanol and methylparaben.

[0031] Preferably, the sun protection product has a sun protection factor (SPF) of 15-50 and a protection factor against UVA (PFA) of 5-20.

[0032] Preferably, it is used in the cosmetics and skincare industry to prepare products with sun protection functions, and in the pharmaceutical industry to prepare drugs for preventing skin diseases caused by ultraviolet radiation.

[0033] Preferably, after ultraviolet irradiation, an inducer is added to the cyanobacteria culture system to further promote the production of natural sunscreen substances. The inducer is pterin, and the addition amount is 0.01-0.1 mmol / L.

[0034] Preferably, the amount of the natural sunscreen substance added to the sunscreen product is adjusted according to the desired sun protection factor (SPF). When preparing a sunscreen product with an SPF of 15-30, the addition amount is 5%-15%; when preparing a sunscreen product with an SPF of 30-50, the addition amount is 15%-20%.

[0035] The natural sunscreen also contains carotenoids, the content of which accounts for 10%-30% of the total weight of the natural sunscreen, and the carotenoids enhance the antioxidant properties of the natural sunscreen.

[0036] The natural sunscreen substance is extracted and purified. The extraction uses an ethanol-water mixed solvent with a volume ratio of 7:3, an extraction temperature of 40-60℃, and an extraction time of 2-6 hours. The purification uses silica gel column chromatography with a hexane-ethyl acetate mixed solvent as the eluent, and a volume ratio of 5:1 between hexane and ethyl acetate.

[0037] Preferably, the sunscreen product further contains an antioxidant, which is one or more of vitamin C and vitamin E, and the antioxidant content is 0.5%-3%, used to enhance the stability and antioxidant capacity of the sunscreen product;

[0038] The sunscreen product has waterproof properties, which are achieved by adding a film-forming agent, namely polyvinylpyrrolidone, at an addition amount of 1%-3%.

[0039] The beneficial effects of this invention are reflected in:

[0040] Methods for inducing cyanobacteria to produce natural sunscreen substances using ultraviolet light:

[0041] This invention provides a sustainable and environmentally friendly method for producing natural sunscreens, using cyanobacteria as a raw material, which reduces reliance on chemically synthesized sunscreens and lowers potential environmental hazards.

[0042] By using specific culture medium components and ultraviolet irradiation conditions, cyanobacteria can be effectively induced to produce natural sunscreen substances, thereby improving production efficiency and yield.

[0043] Pulsed ultraviolet irradiation and specific wavelength and intensity settings can both stimulate cyanobacteria to synthesize sunscreen substances and reduce excessive damage to cyanobacterial cells, thus ensuring the growth and metabolism of cyanobacteria.

[0044] Controlling the growth stages of the cyanobacteria culture system and ensuring ultraviolet irradiation during the logarithmic growth phase improved the production efficiency of natural sunscreen substances.

[0045] Stirring helps ensure even exposure to ultraviolet light, improving the consistency of the reaction.

[0046] The use of a photobioreactor provides suitable light conditions, which is conducive to the growth of cyanobacteria and the synthesis of natural sunscreen substances.

[0047] The addition of the inducer pterin further promoted the production of natural sunscreen substances, increasing yield.

[0048] Natural sunscreen ingredients:

[0049] It contains mycotoxin amino acids (MAAs) and carotenoids, and has good UV absorption properties, which can effectively protect the skin from UV damage.

[0050] The high content of MAAs (Mass Acids) accounts for 30%-70% of the total weight of natural sunscreen substances, ensuring the product's sun protection effect.

[0051] It absorbs ultraviolet light in the wavelength range of 280-400nm, and has a particularly high ultraviolet absorption coefficient in the wavelength range of 310-360nm, providing broad-spectrum ultraviolet protection.

[0052] The presence of carotenoids enhances the antioxidant properties of natural sunscreens, helping to protect the skin from free radical damage.

[0053] Through extraction and purification, the purity and quality of natural sunscreen substances are improved, and the potential irritation of impurities to the skin is reduced.

[0054] Sunscreen products containing natural sunscreen ingredients:

[0055] With natural sunscreen ingredients as the main components, the product reduces skin irritation and improves safety.

[0056] The sunscreen product is in the form of an emulsion, which has good spreadability and a pleasant feel on the skin, and is easily absorbed by the skin.

[0057] The oil phase, water phase, emulsifiers, and preservatives in the formula enable the product to have good stability and moisturizing properties.

[0058] The amount of natural sunscreen ingredients added can be adjusted according to the required sun protection factor to meet the needs of different consumers for sun protection.

[0059] Sunscreen products with a sun protection factor (SPF) of 15-50 and a protection factor against UVA (PFA) of 5-20 provide effective protection against ultraviolet rays and reduce the risk of sunburn and tanning.

[0060] The addition of antioxidants enhances the stability and antioxidant capacity of sunscreen products, helping to protect the skin from oxidative damage.

[0061] The addition of film-forming agents gives sunscreen products waterproof properties and extends the duration of sun protection, making them suitable for use during outdoor activities and when sweating.

[0062] Application areas:

[0063] In the cosmetics and skincare industry, the production methods of this natural sunscreen substance and the sunscreen products provide consumers with safer and more effective sun protection options, helping to meet the market's demand for natural and environmentally friendly cosmetics.

[0064] In the medical field, natural sunscreens can be used to prepare drugs that prevent skin diseases caused by ultraviolet radiation, providing new treatment and prevention methods for skin health and playing an important role in reducing the incidence of skin diseases.

[0065] In summary, the method for producing natural sunscreen substances from cyanobacteria using ultraviolet light and its applications not only provide a new and sustainable approach to the production of sunscreen products, but also produce natural sunscreen substances and products with excellent performance and safety, showing broad application prospects and significant social importance in the cosmetics, skincare, and pharmaceutical fields. This method helps promote sustainable development in the industry, protects consumers' skin health, and also makes a positive contribution to environmental protection. Attached Figure Description

[0066] In the attached diagram:

[0067] Figure 1 This is a flowchart of the method for producing natural sunscreen substances from cyanobacteria using ultraviolet light, according to the present invention. Detailed Implementation

[0068] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. The described embodiments are merely some, not all, of the invention. Unless otherwise specified, the embodiments and features described in this application can be combined with each other. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.

[0069] Furthermore, "multiple" refers to two or more. The technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of a person skilled in the art to implement them. When a combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed in the invention.

[0070] Please refer to the instruction manual appendix. Figure 1 This invention provides a method for inducing cyanobacteria to produce natural sunscreen substances using ultraviolet light and its application:

[0071] Example 1: A method for inducing cyanobacteria to produce natural sunscreen substances using ultraviolet light

[0072] Provides cyanobacteria culture systems:

[0073] Prepare the culture medium, with each liter containing: 1.0 g sodium nitrate, 0.05 g potassium dihydrogen phosphate, 0.1 g magnesium sulfate, 0.03 g calcium chloride, 3 ml trace element solution, and the remainder being water. Each liter of the trace element solution contains: 1.0 g boric acid, 0.1 g manganese chloride, 0.05 g zinc sulfate, 0.01 g sodium molybdate, 0.001 g copper sulfate, and 0.001 g cobalt chloride.

[0074] Nostoc desertis was inoculated as a cyanobacterial strain.

[0075] Growth stage control in cyanobacteria culture systems:

[0076] Before ultraviolet irradiation, the cyanobacteria culture system was cultured to the logarithmic growth phase. The growth stage of the cyanobacteria was determined by measuring the optical density value at 680 nm (OD680). When OD680 reached 0.3-0.8, it was determined to be in the logarithmic growth phase.

[0077] Apply ultraviolet light to the cyanobacteria culture system:

[0078] Use ultraviolet light with a wavelength of 280-320nm.

[0079] The intensity of ultraviolet radiation is 5-20 microwatts per square centimeter.

[0080] Pulsed irradiation was used, with a pulse frequency of 1-5 Hz, each pulse lasting 1-10 seconds, and a total irradiation time of 1-6 hours. During irradiation, the cyanobacteria culture system was stirred at a speed of 50-200 rpm.

[0081] Continue culturing cyanobacteria after ultraviolet irradiation:

[0082] Continue culturing at 25°C.

[0083] The pH value should be controlled between 7.5 and 9.0.

[0084] The culture period continues for 24-72 hours.

[0085] The cyanobacteria cultivation system uses a photobioreactor during the cultivation process, with a light intensity of 100-500 micromolar photons / m²·second and a light-dark cycle of 12 hours:12 hours.

[0086] Add inducing agent:

[0087] After ultraviolet irradiation, pterin was added to the cyanobacteria culture system as an inducer at a concentration of 0.01-0.1 mmol / L to further promote the production of natural sunscreen substances.

[0088] Example 2: Properties and Applications of Natural Sunscreen Substances

[0089] Composition and properties of natural sunscreens:

[0090] The natural sunscreen substance produced according to the method of Example 1 contains mycotoxin-like amino acids (MAAs), and the content of MAAs accounts for 30%-70% of the total weight of the natural sunscreen substance.

[0091] This natural sunscreen also contains carotenoids, which account for 10%-30% of the total weight of the natural sunscreen, and carotenoids enhance the antioxidant properties of the natural sunscreen.

[0092] Natural sunscreens absorb ultraviolet rays in the wavelength range of 280-400nm, and the ultraviolet absorption coefficient in the wavelength range of 310-360nm is 100-500L / (mol·cm).

[0093] Extraction and purification of natural sunscreen substances:

[0094] Natural sunscreen substances were extracted using an ethanol-water mixed solvent with a volume ratio of 7:3 for ethanol to water, at an extraction temperature of 40-60℃, and for an extraction time of 2-6 hours.

[0095] The extracted natural sunscreen substance was purified by silica gel column chromatography, with hexane-ethyl acetate mixed solvent as the eluent, and the volume ratio of hexane to ethyl acetate was 5:1.

[0096] Application of natural sunscreen ingredients in sunscreen products:

[0097] The extracted and purified natural sunscreen substances were used in the preparation of sunscreen products. The sunscreen product was in the form of an emulsion, and its ingredients were as follows:

[0098] Natural sunscreen ingredients: 10%

[0099] Oil phase composition: 20%, containing olive oil and squalane.

[0100] Aqueous phase composition: 60%, including deionized water, glycerin, and sodium hyaluronate.

[0101] Emulsifier: 3%, consisting of polysorbate-80 and cetearyl alcohol polyether-20.

[0102] Preservatives: 0.5%, consisting of phenoxyethanol and methylparaben.

[0103] Antioxidants: 1%, consisting of vitamin C and vitamin E.

[0104] Film-forming agent: 2%, which is polyvinylpyrrolidone.

[0105] This sunscreen product has a sun protection factor (SPF) of 30 and a protection factor against UVA (PFA) of 10. The amount of natural sunscreen ingredients added is adjusted according to the required SPF. When preparing sunscreen products with an SPF of 15-30, the amount of natural sunscreen ingredients added is 5%-15%; when preparing sunscreen products with an SPF of 30-50, the amount of natural sunscreen ingredients added is 15%-20%.

[0106] Example 3: Application of the method for inducing cyanobacteria to produce natural sunscreen substances using ultraviolet light

[0107] Applications in the cosmetics and skincare industry:

[0108] The natural sunscreen substance produced according to the method of Example 1 is applied to the preparation of cosmetics and skin care products to produce products with sun protection function, such as sunscreen, makeup base, etc.

[0109] Applications in the pharmaceutical field:

[0110] Natural sunscreen substances are used to prepare drugs that prevent skin diseases caused by ultraviolet radiation, such as drugs to prevent sunburn, skin aging, and skin cancer.

[0111] Based on the preparation methods of the above embodiments, the following data table shows the improvement in content achieved by using the method of the present invention compared to the traditional method:

[0112] method Natural sunscreen ingredient content (%) Content increase (%) Traditional UV methods 30 0 Method of the present invention 60 100

[0113] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

[0114] For those skilled in the art, the present invention is not limited to the details of the exemplary embodiments described above, and can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0115] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A method for producing natural sunscreen substances by ultraviolet light induction of cyanobacteria, characterized in that: a cyanobacteria culture system is provided; the cyanobacteria culture system comprises a specific culture medium, the components of the culture medium comprise, per liter, 0.5-2.0 grams of sodium nitrate, 0.02-0.1 grams of potassium dihydrogen phosphate, 0.05-0.2 grams of magnesium sulfate, 0.01-0.05 grams of calcium chloride, 1-5 milliliters of trace element solution, and the balance is water; the trace element solution comprises, per liter, 0.5-2.0 grams of boric acid, 0.05-0.3 grams of manganese chloride, 0.01-0.1 grams of zinc sulfate, 0.005-0.02 grams of sodium molybdate, 0.0005-0.002 grams of copper sulfate, and 0.0005-0.002 grams of cobalt chloride; the cyanobacteria strain is Nostoc commune Vauch; the cyanobacteria culture system is irradiated with ultraviolet light of a specific wavelength and intensity; the wavelength of the ultraviolet light is 280-320 nm; the intensity of the ultraviolet light is 5-20 microwatts per square centimeter; the ultraviolet light irradiation is pulsed, the pulse frequency is 1-5 hertz, each pulse lasts for 1-10 seconds, and the total irradiation time is 1-6 hours; after ultraviolet light irradiation, the cyanobacteria are further cultured to produce natural sunscreen substances; the temperature of the further culture is 25-35°C; the pH value of the further culture is 7.5-9.0; the time of the further culture is 24-72 hours. The cyanobacteria culture system is in the logarithmic growth phase before ultraviolet light irradiation, wherein the growth phase of the cyanobacteria is determined by measuring the optical density value at 680 nm (OD680), and the logarithmic growth phase is determined when OD680 reaches 0.3-0.8; During ultraviolet light irradiation, the cyanobacteria culture system is stirred at a stirring speed of 50-200 revolutions per minute; The cyanobacteria culture system uses a photobioreactor during culture, and the photobioreactor has an illumination intensity of 100-500 micromoles of photons per square meter per second and a light-dark cycle of 12 hours:12 hours. The natural sunscreen substances contain mycosporine-like amino acids (MAAs), and the content of MAAs accounts for 30%-70% of the total weight of the natural sunscreen substances; The natural sunscreen substances absorb ultraviolet light with a wavelength range of 280-400 nm, and the ultraviolet light absorption coefficient in the wavelength range of 310-360 nm is 100-500 L / (mol·cm). The sunscreen product is in the form of an emulsion, and the emulsion comprises the following components: natural sunscreen substances: 5%-20%; oil phase ingredients: 10%-30%, which comprise one or more of olive oil and squalane; aqueous phase ingredients: 40%-70%, which comprise one or more of deionized water, glycerol, and sodium hyaluronate; emulsifiers: 1%-5%, which are one or more of polysorbate-80 and cetostearyl alcohol polyether-20; preservatives: 0.1%-1%, which are one or more of phenoxyethanol and hydroxybenzoate.

2. The method of claim 1, wherein the ultraviolet light is induced cyanobacteria to produce natural sunscreen substances, characterized in that, ​ ​ ​ 3. A natural sunscreen material produced by the method of any one of claims 1-2, wherein, ​ ​ 4. A sunscreen product comprising the natural sunscreen substance of claim 3, characterized in that, ​ ​ ​ ​ ​ ​ 5. The natural sunscreen product of claim 4, wherein the natural sunscreen substance is selected from the group consisting of aloe vera, chamomile, green tea, and a combination thereof. The sunscreen product has a sunscreen index (SPF) of 15-50 and a UVA protection factor (PFA) of 5-20.

6. Use of the method for the production of natural sunscreen substances by UV-induced cyanobacteria according to any one of claims 1 to 2, characterized in that: The natural sunscreen material is used for preparing products with sunscreen function in the field of cosmetics and skin care products, and is used for preparing medicines for preventing skin diseases caused by ultraviolet rays in the field of medicine.

7. The method of claim 1, wherein the ultraviolet light is induced cyanobacteria to produce natural sunscreen substances, characterized in that, After ultraviolet irradiation, an inducer is added to the cyanobacteria culture system to further promote the production of natural sunscreen substances, and the inducer is pterin, and the addition amount is 0.01-0.1 mmol / L.

8. Use of natural sunscreen substances according to claim 3 for the preparation of sunscreen products, characterized in that, The addition amount of the natural sunscreen substance in the sunscreen product is adjusted according to the required sunscreen index, when preparing a sunscreen product with SPF of 15-30, the addition amount is 5%-15%, when preparing a sunscreen product with SPF of 30-50, the addition amount is 15%-20%; The natural sunscreen substance also contains carotenoids, the content of carotenoids accounts for 10%-30% of the total weight of the natural sunscreen substance, and the carotenoids have an enhancing effect on the antioxidant properties of the natural sunscreen substance; The natural sunscreen substance is extracted and purified, the extraction uses an ethanol-water mixed solvent, the volume ratio of ethanol to water is 7:3, the extraction temperature is 40-60 DEG C, and the extraction time is 2-6 hours; the purification uses a silica gel column chromatography method, and the eluent is a n-hexane-ethyl acetate mixed solvent, the volume ratio of n-hexane to ethyl acetate is 5:

1.

9. The natural sunscreen product of claim 4, wherein, The sunscreen product also contains an antioxidant, the antioxidant is one or more of vitamin C and vitamin E, the content of the antioxidant is 0.5%-3%, which is used to enhance the stability and antioxidant capacity of the sunscreen product; The sunscreen product has waterproof performance, which is realized by adding a film-forming agent, and the film-forming agent is polyvinylpyrrolidone, and the addition amount is 1%-3%.

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