An online ATP detection device

Abstract

This invention relates to the field of microbial detection equipment technology, specifically to an online ATP detection device. The specific technical solution is as follows: An online ATP detection device includes a housing. A triaxial slide, separated from the bottom of the housing, is disposed within the housing. The triaxial slide includes mutually perpendicular x-axis, y-axis, and z-axis slides. The x-axis slide is located on one side of the housing along its width. A syringe pump is mounted on the z-axis slide, and an injection needle is mounted on the syringe pump. A stirrer, a reagent bottle holder, a reaction cell, and a photoelectric detector are arranged within the housing along the direction of the y-axis slide. This invention solves the problems of sample residue in the peristaltic pump inlet tube and the inability to accurately control the injection volume in existing technologies.

Description

Technical Field

[0001] This invention relates to the field of microbial detection equipment technology, specifically to an online ATP detection device. Background Technology

[0002] The activated sludge process is the most commonly used wastewater treatment method in wastewater treatment plants. Microorganisms are the main functional components of the activated sludge process and are the primary participants in the pollutant removal process; their activity and composition directly affect the pollutant removal efficiency. Therefore, real-time monitoring of microbial activity is of great significance for improving wastewater treatment performance, guiding process optimization and control, and ensuring stable process operation.

[0003] ATP fluorescence detection is a rapid detection technique based on the bioluminescence principle of fireflies. It involves the reaction of luciferin with ATP, catalyzed by luciferase, in the presence of oxygen, resulting in fluorescence. The fluorescence intensity of this technique is directly proportional to microbial activity; therefore, the ATP content can be used as an indicator of microbial activity.

[0004] Currently, the only microbial activity detection equipment on the market for ATP is the portable ATP analyzer, which is primarily designed for testing in the food service and medical sectors. However, real-time monitoring of the microbial status of wastewater biological treatment systems is crucial. Current online detection devices for microbial ATP activity in activated sludge generally use peristaltic pumps to dispense reagents. This method suffers from reagent residue in the sample inlet tube, leading to inaccurate control of the sample volume. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides an online ATP detection device that solves the problems of sample residue in the peristaltic pump inlet tube and the inability to accurately control the injection volume in existing technologies.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] This invention discloses an online ATP detection device, comprising a housing, wherein a triaxial slide is disposed inside the housing and separated from the bottom of the housing. The triaxial slide includes a mutually perpendicular x-axis slide, a y-axis slide, and a z-axis slide. The x-axis slide is disposed on one side of the housing in the width direction. An injection pump is disposed on the z-axis slide, and an injection needle is disposed on the injection pump. A stirrer, a reagent bottle holder, a reaction cell, and a photoelectric detector are arranged inside the housing along the setting direction of the y-axis slide.

[0008] Preferably, the reagent bottle holder is provided with at least one reagent bottle, and is arranged along the direction of the y-axis slide.

[0009] Preferably, the box is equipped with a reagent storage mechanism, which includes a refrigerator. The refrigerator contains vials, and the top of the refrigerator has a dispensing port corresponding to the top of the vials.

[0010] Preferably, the refrigerator is provided with a fixing frame for fixing vials. The fixing frame includes a U-shaped frame installed inside the refrigerator with its opening facing upward. The two sides of the U-shaped frame are fixed to the inner wall of the refrigerator. An n-shaped frame with its opening facing downward is installed inside the U-shaped frame. The n-shaped frame is fixed to the U-shaped frame. The vial is fixed to the top of the n-shaped frame by a fixing cylinder with a top opening.

[0011] Preferably, an annular elastic sleeve is provided on the inner wall of the fixed cylinder.

[0012] Preferably, the photoelectric detector and the stirrer are connected to a peristaltic pump, and the reagent bottle, the reaction cell, the photoelectric detector, and the stirrer are all added and the reagents are extracted via an injection needle.

[0013] Preferably, the stirrer includes a sample cell and a stirring mechanism disposed within the sample cell, the photoelectric detector includes a photon counter and a light-shielding box assembly disposed at the detection end of the photon counter, and a support is provided at the bottom of the photon counter.

[0014] Preferably, the light-shielding box assembly includes a housing and a light-shielding box disposed inside the housing corresponding to the photon counter detection end. A light-shielding plate driven by an electric actuator is disposed between the light-shielding box and the photon counter detection end. The electric actuator is disposed on a fixed base.

[0015] Preferably, a detection tube corresponding to the photon counter detection end is arranged horizontally inside the light-shielding box. The top of the detection tube is provided with a sample inlet, and the bottom is provided with a sample outlet tube. The sample outlet tube is connected to a light-shielding conduit, and the light-shielding conduit is connected to a solenoid valve. A plug is provided at the outward end of the detection tube.

[0016] The present invention has the following beneficial effects:

[0017] 1. The ATP online detection device disclosed in this invention changes the reagent extraction method from a peristaltic pump to an injection needle, solving the problem of inaccurate reagent volume caused by sample residue in the pipeline, and realizing real-time detection of ATP in sewage.

[0018] 2. The ATP online detection device disclosed in this invention can automatically clean each detection component after the detection is completed, in preparation for the next detection. This effectively removes residual wastewater samples and ensures the accuracy of subsequent detections. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of the present invention;

[0020] Figure 2 for Figure 1 Top view after removing the top cover of the enclosure;

[0021] Figure 3 for Figure 1 Front view after removing the front panel of the enclosure;

[0022] Figure 4 This is a schematic diagram of the light-shielding box assembly;

[0023] Figure 5 A cross-sectional view of the light-shielding box assembly housing in the direction shown.

[0024] Figure 6 A schematic diagram of the light-shielding box assembly after one side of the outer casing has been removed;

[0025] Figure 7 This is a schematic diagram of a refrigerator.

[0026] Figure 8 Blank value determination for online detection devices;

[0027] Figure 9 The ATP standard curve for the online ATP detection device;

[0028] Figure 10 To ensure the stability of continuous sample measurement in the ATP online detection device;

[0029] Figure 11 A comparison of ATP testing using an online ATP detection device and an ELISA reader;

[0030] Figure 12 Comparison of luminescence intensity between online ATP detection device and enzyme-linked immunosorbent assay (ELISA) reader for domestic sewage, activated sludge, algal solution, and bacterial suspension;

[0031] Figure 13 A comparison chart of actual and predicted effluent COD (without ATP added);

[0032] Figure 14 A comparison chart of actual and predicted effluent COD (with ATP added);

[0033] Figure 15 This is a scatter plot of the characteristic density.

[0034] Figure 16 A bar chart showing the importance of features;

[0035] In the diagram: 1. Box body; 2. X-axis slide; 3. Y-axis slide; 4. Injection pump; 5. Injection needle; 6. Reagent bottle support; 7. Reaction cell; 8. Reagent bottle; 9. Refrigerator; 10. Vial; 11. Liquid dispensing port; 12. U-shaped frame; 13. N-shaped frame; 14. Fixing cylinder; 15. Support; 16. Solenoid valve support; 17. Peristaltic pump; 18. Sample cell; 19. Stirring mechanism; 20. Outer shell; 21. Electric actuator; 22. Light shield; 23. Fixing base; 24. Detection tube; 25. Sample inlet; 26. Solenoid valve; 27. Photon counter; 28. Light shield box; 29. ​​Light shield conduit; 30. Z-axis slide. Detailed Implementation

[0036] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art.

[0037] refer to Figures 1-7 This invention discloses an online ATP detection device, comprising a housing 1. A three-axis slide, separated from the bottom of the housing 1, is disposed within the housing 1. The three-axis slide includes a mutually perpendicular x-axis slide 2, a y-axis slide 3, and a z-axis slide 30. The x-axis slide 2 is disposed inside the housing 1. A syringe pump 4 is mounted on the z-axis slide 3 to drive the syringe pump to move up and down. The z-axis slide and the syringe pump move along the y-axis slide, which preferably corresponds to the door of the housing. An injection needle 5 is mounted on the syringe pump 4. A stirrer, a reagent bottle holder 6, a reaction chamber 7, and a photoelectric detector are arranged inside the housing 1 along the direction of the y-axis slide 3. That is, the direction of the stirrer, reagent bottle holder 6, reaction chamber 7, and photoelectric detector is the same as the direction of the y-axis slide. Through the cooperation of the x, y, and z-axis slides, the injection needle moves among the stirrer, reagent bottle holder 6, reaction chamber 7, and photoelectric detector for sampling.

[0038] Furthermore, at least one reagent bottle 8 is provided on the reagent bottle holder 6, and is arranged along the direction of the y-axis slide 3. Reagent bottles of different volumes can be placed on the reagent bottle holder.

[0039] Furthermore, the housing 1 is equipped with a reagent storage mechanism, which includes a refrigerator 9. The refrigerator 9 contains vials 10, and the top of the refrigerator 9 is provided with a sampling port 11 corresponding to the top of the vial 10. An injection needle is inserted into the vial through the sampling port to take a sample.

[0040] Furthermore, the refrigerator 9 is equipped with a fixing bracket for securing the vial 10. The fixing bracket includes a U-shaped frame 12 with its opening facing upwards, located inside the refrigerator 9. The two sides of the U-shaped frame 12 are fixed to the inner wall of the refrigerator 9. An n-shaped frame 13 with its opening facing downwards is located inside the U-shaped frame 12. The n-shaped frame 13 and the U-shaped frame 12 are fixed together with bolts. The vial 10 is fixed to the top of the n-shaped frame 13 via a fixing cylinder 14 with an opening at the top. It should be noted that the U-shaped frame can also be two unconnected side panels fixed inside the refrigerator, while the n-shaped frame can be a single plate fixed between the two side panels. The specific configuration depends on actual needs. The combination of the U-shaped and n-shaped frames improves the stability of the fixing bracket.

[0041] Furthermore, an annular elastic sleeve is provided on the inner wall of the fixing cylinder 14. The inner diameter of the fixing cylinder is larger than that of the vial. The annular elastic sleeve on its inner wall has a certain amount of deformation, and the amount of deformation determines the diameter range of the vial that can be fixed. It can be set according to the specific needs. The annular elastic sleeve can be made of rubber or a micro-airbag. Furthermore, a peristaltic pump 17 is connected to the photoelectric detector and the stirrer. The reagent bottle 8, the reaction cell 7, the photoelectric detector, and the stirrer are all sampled and extracted through the injection needle 5. The peristaltic pump of the present invention is set at the inlet and outlet of the sample cell with the stirring mechanism; and at the outlet of the reaction cell of the photoelectric detector. In the specific experiments of the present invention, the detection reagent, the extraction reagent, and the extraction of the mixed sample are all completed through the injection needle. It should be noted that the present invention uses more than one peristaltic pump. For example, one peristaltic pump pumps the activated sludge-water mixture into the sample cell for stirring by the stirring mechanism, and another peristaltic pump extracts the sludge-water mixture from the sample cell. Of course, the equipment and instruments used will be cleaned by pumping clean water in through a peristaltic pump. The specific settings of the pipes on the peristaltic pump can be set according to actual needs. For example, after the entire testing device is used, the cleaning tube can be placed into the corresponding instrument and clean water can be pumped in for cleaning.

[0042] Furthermore, the stirrer includes a sample pool 18 and a stirring mechanism 19 disposed within the sample pool 18. The stirring mechanism is existing technology and will not be described in detail. The photoelectric detector includes a photon counter 27 and a light-shielding box assembly disposed at the detection end of the photon counter 27. A bracket 15 is disposed at the bottom of the photon counter 27, and the photoelectric detector is fixed in the housing by the bracket.

[0043] Furthermore, the light-shielding box assembly includes a housing 20 and a light-shielding box 28 disposed within the housing 20 corresponding to the detection end of the photon counter 27. A light-shielding plate 22 driven by an electric actuator 21 is disposed between the light-shielding box 28 and the detection end of the photon counter 27. The electric actuator 21 is mounted on a fixed base 23. After detection is completed, repeated detection can be stopped by driving the light-shielding plate to block the detection end of the photon counter 27.

[0044] Specifically: The light-shielding box 28 has a detection tube 24 corresponding to the detection end of the photon counter 27 arranged horizontally inside. The top of the detection tube 24 has a sample inlet 25 and the bottom has a sample outlet tube. The sample outlet tube is connected to a light-shielding conduit 29. The light-shielding conduit 29 is connected to a solenoid valve 26. The solenoid valve controls the discharge of the sample in the detection tube. The support 15 is equipped with a solenoid valve support 16.

[0045] When using this invention, the injection needle 5 is moved to the positions of each reagent bottle 8, reaction cell 7, sample cell 18, etc. by a three-axis slide, and the x, y, z coordinates of each component are recorded. In the subsequent detection process, the injection needle will be moved according to the position of different components to accurately extract / inject liquid.

[0046] The specific detection method of this invention is as follows: First, the activated sludge-water mixture is pumped into sample tank 18 using a 7600-OEM peristaltic pump. The sample tank 18 is then stirred to ensure uniform mixing. Next, according to the previously obtained and set sample tank coordinates, the injection needle is moved into the sample tank using a triaxial slide. The injection pump 4 controls the injection needle 5 to draw a quantitative sample into reaction tank 7. Subsequently, the injection needle is moved to a reagent bottle (containing the extraction reagent) to draw a quantitative extractant into reaction tank 7. The liquid in the reaction tank is mixed by controlling the injection needle's pumping motion. After the microbial cells in the activated sludge have lysed, the supernatant is drawn into a detection tube. The injection needle is then moved to a vial in a refrigerator to draw a quantitative detection reagent into the detection tube. The liquid in the detection tube is mixed by pumping the injection needle. Finally, a photon counter runs for 30 seconds to detect the average photon value during this period. The measured photon value is displayed on the software system interface (this photon value is also saved in a local disk file). Subsequently, based on the previously determined standard curve, the detected photon value is converted into the concentration of ATP in the sample. After sample testing is completed, ultrapure water is drawn from the reagent bottle (containing water) using a syringe to clean all testing components in preparation for the next test. It is important to note that the syringe needle must be cleaned after each use to draw reagent or sample.

[0047] Working principle of the invention:

[0048] (1) The PLC control system monitors the status of each unit and displays it on the display screen (set on the cabinet). If there is no abnormality in each unit, the light leakage detection begins and the photon counter detects the light emission value (RLU). If the light emission value (RLU) is higher than 200, the PLC control system will issue a light leakage alarm. If the light emission value (RLU) is lower than 200, the light shielding of the detection unit is in normal condition and the display screen shows that the equipment is normal.

[0049] (2) The PLC control system controls the operation of each electrical device. The injection pump draws the sample into the reaction cell, and the injection needle draws the extractant from the reagent bottle into the reaction cell. After the reaction is completed, the supernatant of the reaction cell is drawn into the detection tube through the injection needle. The injection needle draws 100μl of ATP detection reagent from the refrigerator into the detection tube. The liquid in the detection tube is mixed by the injection needle. The photon counter is turned on to start the detection. The display screen shows the detection result.

[0050] (3) After the test is completed, the PLC control system controls the peristaltic pump to draw ultrapure water from the reagent bottle to the pure water pool. The injection needle is cleaned in the pure water pool by the moving injection pump. After cleaning, the water in the pure water pool is drawn out by another peristaltic pump, and then ultrapure water is injected into the pure water pool by the peristaltic pump. Then the injection needle is used to draw ultrapure water into the reaction pool and the detection pool to clean the fluid passages of each module for the next use.

[0051] The present invention will be further described below with reference to specific embodiments.

[0052] The main reagents used in this invention are as follows: Tris(hydroxymethyl)aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), magnesium acetate (Mg(CH3COO)2), benzalkonium bromide (BAB), dithiothreitol (DTT), bovine serum albumin (BSA), glutathione (GSH), luciferin (D-Luciferin, Ln), luciferase firefly (Fl), dextran DEAE-DX, sucrose, adenosine triphosphate (ATP), sodium hydroxide, hydrochloric acid, etc. (all reagents used are of analytical grade).

[0053] It should be noted that the main detection instruments used in the experiments of this invention are the full-wavelength microplate reader (ThermoScientific) and the above-disclosed online ATP detection device, and the detection results are all given in relative light units (RLU).

[0054] Example 1: Performance Evaluation of Online ATP Detection Device

[0055] (1) Determination of instrument detection limit

[0056] The detection limit of the online microbial activity detection device was determined by measuring the blank value of the device. Specifically, ultrapure water was used to measure photon values ​​continuously for 1 minute at a frequency of 1 time / second. Three sets of ultrapure water photon values ​​were measured, and their average value was used as the blank value to determine the detection limit. Results are shown below. Figure 8 As shown, by Figure 8 The results showed that the three groups of ultrapure water samples tested had a minimum photon value of 33, a maximum of 154, and an average of 79.57. The blank value was recorded as 79.57, and three times the blank value (239) was used as the lower limit of detection for the device. The test results indicate that the device has a low detection limit and can detect ATP content in low-concentration samples. Based on the blank value measurements, 239 was used as the lower limit of detection for the device, and the detection limit of the device's photon counter was set at 2 × 10⁻⁶. 7 As the upper limit of the device's detection range, therefore [239, 2×10 7 [ ] is the measurement range of sample photon values ​​of the online microbial activity detection device disclosed in this invention.

[0057] (2) Determination of ATP standard curve

[0058] The photon value of this device is compared with the standard curve of ATP using 1 mmol·L⁻¹. -1 The ATP standard solution (prepared by accurately weighing 1 mg of ATP powder and adding it to 1.9717 mL of sterile Tris buffer) was prepared using 20 mmol·L⁻¹. -1 Tris buffer was diluted to obtain 2000, 4000, 6000, 8000, and 10000 nmol·L⁻¹. -1 The standard solutions were prepared. The photon values ​​of each concentration of the standard solution were measured using a self-made online ATP detection device. The test was repeated three times, and the average value was used to plot a standard curve with ATP concentration on the x-axis and RLU on the y-axis.

[0059] See results Figure 9 ,Depend on Figure 9 It can be known that 2000-10000 nmol·L -1 The equation for the ATP standard curve within the specified range is y = 1012x + 6.45 * 10. 6 R 2 =0.9994; where 10000 nmol·L -1 The photon value of ATP is 16,607,616, which determines the detection limit of this device to be 10,000 nmol·L⁻¹. -1 Samples exceeding the detection limit should be diluted before automatic detection of ATP content.

[0060] (3) Instrument stability test

[0061] Concentrations of 2000, 4000, 6000, 8000, and 10000 nmol·L were selected. -1 The ATP standard solution samples were measured 10 times repeatedly, and the data repeatability and detection stability of the online detection device were evaluated by relative standard deviation (RSD).

[0062] See results Figure 10 ,Depend on Figure 10 The relative deviations of the detection results from low to high concentrations were 3.6%, 4.1%, 1.3%, 1.8%, and 1.4%, respectively. Among the repeated tests of the above five groups of samples, the highest relative deviation was 4.1%, indicating that the detection device has strong stability and high repeatability of the results at the same concentration. Furthermore, high-concentration samples (6000, 8000, and 10000 nmol·L⁻¹) showed good performance. -1 The relative deviation of the measured values ​​was smaller than that of the low concentration samples (2000, 4000 nmol·L⁻¹). -1 The relative deviation indicates that the online detection method provided by this device has better stability for the detection of ATP in samples with higher concentrations.

[0063] (4) Instrument accuracy evaluation

[0064] Using an online ATP detection device and a microplate reader, ATP concentrations of 2000, 4000, 6000, 8000, and 10000 nmol·L⁻¹ were analyzed. -1 Five concentration gradient ATP standard solutions were simultaneously measured, and Pearson correlation analysis was performed to evaluate the accuracy of the instrument.

[0065] See results Figure 11 ,Depend on Figure 11 The correlation analysis results show that the correlation between the ATP values ​​detected by the two methods is 0.9960 (p < 0.01), which proves that the two methods have a high degree of correlation with the sample detection results, indicating that the measured values ​​of this online ATP detection device are accurate and reliable.

[0066] (5) Evaluation of instrument suitability

[0067] Five concentration tests were conducted on four types of samples: domestic sewage, activated sludge, algal solution, and bacterial suspension. Since the initial concentrations of these samples were unknown, a gradient dilution method was used. Samples of 0.5, 0.4, 0.3, 0.2, and 0.1 mL were taken and diluted with the corresponding volume of ultrapure water to 1 mL to prepare test samples of different concentrations. Finally, the luminescence intensity of the samples was measured using an online ATP detection device and a microplate reader.

[0068] See results Figure 12 ,Depend on Figure 12The Pearson correlations for the four sample groups were 0.9940, 0.9913, 0.9902, and 0.9908 (p < 0.01), demonstrating a significant correlation between the two variables. These experimental results further validate the accuracy of this online detection device and also indicate its broad applicability for detecting ATP content in various samples.

[0069] Example 2: ATP-based prediction of effluent COD

[0070] To further explore the crucial role of ATP in model prediction, this invention designed two sets of different characteristic variables for comparative analysis: the first group, "without ATP," included influent COD, total nitrogen (TN), total phosphorus (TP), and ammonia nitrogen (NH3-N); the second group, "with ATP," included influent COD, total nitrogen, total phosphorus, ammonia nitrogen, ATP in the anoxic tank, ATP in the anaerobic tank, and ATP in the aerobic tank. The MLP algorithm was used to predict effluent COD. Results are shown below. Figure 13 , 14 As shown.

[0071] The effluent COD prediction results showed that the MAE was 0.402 mg / L and the RMSE was 0.510 mg / L in the "no ATP added" group. 2 It is 0.502. (By...) Figure 13 It can be seen that there is a certain gap between the predicted effluent COD and the actual effluent COD data, indicating that the prediction effect needs to be improved.

[0072] In comparison, by Figure 14 It can be seen that the performance of the "ATP-added" group was significantly improved, and its MAE decreased to 0.243 mg / L, RMSE was 0.321 mg / L, and R... 2 The accuracy was improved to 0.802. This indicates that introducing ATP as a feature variable significantly improved the model's prediction accuracy for effluent COD, making the prediction results closer to the actual data, and effectively enhancing the model's reliability and accuracy. This is likely because ATP can effectively indicate the metabolic activity of microorganisms, which greatly affects COD degradation performance; therefore, the inclusion of ATP data can effectively improve the prediction results for effluent COD.

[0073] Figure 15 and 16 This study revealed the differences in the contribution of various parameters to the prediction model. Among them, the aerobic tank had the highest average absolute value of ATP SHAP (SHAP|mean value| above 0.35), demonstrating its importance in predicting effluent COD. Figure 13It can be seen that ATP contributes the most to the aerobic tank, indicating that the ATP concentration in the aerobic tank has a significant impact on the model's prediction results. This is likely mainly because the magnitude of ATP reflects the metabolic state of the microorganisms, and the metabolic state of the microorganisms in the aerobic tank plays a crucial role in COD removal. Therefore, ATP in the aerobic tank has a significant impact on the effluent COD. This result is also consistent with the prediction results of two sets of models with different characteristics. The ATP values ​​in the aerobic tank decrease from red to blue (from left to right), while the corresponding SAP values ​​increase from small to large; this indicates a negative correlation between ATP in the aerobic tank and effluent COD, meaning that as the ATP in the aerobic tank increases, the effluent COD decreases. This result shows that the higher the ATP of the activated sludge in the aerobic tank, the stronger its COD degradation capacity. Conversely, as the influent COD increases (from left to right), its SAP value also increases, indicating a positive correlation between influent COD and effluent COD, a result consistent with common sense. Furthermore, from... Figure 16 It is evident that the impact of influent COD on effluent COD is second only to ATP in the aerobic tank, indicating that within a certain range, microbial metabolic activity has a greater impact on effluent COD than influent COD. Therefore, ATP can serve as a key indicator for assessing biological activity in water treatment processes. Furthermore, by monitoring and controlling the ATP level in the aerobic tank, we can more accurately predict and optimize water treatment performance.

[0074] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this invention, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0075] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. An online ATP detection device, comprising a housing (1), characterized in that: The box (1) is provided with a three-axis slide table that is separated from the bottom of the box (1). The three-axis slide table includes a mutually perpendicular x-axis slide table (2), a y-axis slide table (3) and a z-axis slide table (30). The x-axis slide table (2) is located on one side of the width direction inside the box (1). The z-axis slide table (30) is provided with an injection pump (4) and an injection needle (5). The box (1) is provided with a stirrer, a reagent bottle support (6), a reaction tank (7) and a photoelectric detector arranged along the setting direction of the y-axis slide table (3). The stirrer includes a sample cell (18) and a stirring mechanism (19) disposed in the sample cell (18). The photoelectric detector includes a photon counter (27) and a light shielding box assembly disposed at the detection end of the photon counter (27). A bracket (15) is provided at the bottom of the photon counter (27). The light shielding box assembly includes a housing (20) and a light shielding box (28) disposed inside the housing (20) corresponding to the detection end of the photon counter (27). A light shielding plate (22) driven by an electric push rod (21) is disposed between the light shielding box (28) and the detection end of the photon counter (27). The electric push rod (21) is disposed on a fixed base (23). The detection method is as follows: the syringe pump (4) controls the syringe needle (5) to draw a quantitative sample into the reaction tank (7); then, the syringe needle (5) is moved to the reagent bottle (8) to draw a quantitative extractant into the reaction tank (7); the liquid in the reaction tank (7) is mixed by controlling the syringe needle (5); when the microbial cells in the activated sludge have been lysed, the supernatant is drawn into the detection tube (24); then the syringe needle (5) is moved to the vial (10) in the refrigerator (9) to draw a quantitative detection reagent into the detection tube (24), and the liquid in the detection tube (24) is mixed by the syringe needle (5); Finally, the photon counter (27) runs for 30 seconds to detect the average photon value during the detection period. The measured photon value is displayed on the software system interface. Subsequently, the detected photon value is converted into the concentration of ATP in the sample according to the measured standard curve. The light-shielding box (28) is horizontally arranged with a detection tube (24) corresponding to the detection end of the photon counter (27). The top of the detection tube (24) is provided with a sample inlet (25) and the bottom is provided with a sample outlet tube. The sample outlet tube is connected to a light-shielding conduit (29). The light-shielding conduit (29) is connected to a solenoid valve (26). The bracket (15) is provided with a solenoid valve bracket (16).

2. The ATP online detection device according to claim 1, characterized in that: At least one reagent bottle (8) is provided on the reagent bottle holder (6) and is arranged along the direction of the y-axis slide (3).

3. The ATP online detection device according to claim 1, characterized in that: The box (1) is equipped with a reagent storage mechanism, which includes a refrigerator (9). The refrigerator (9) is equipped with a vial (10), and the top of the refrigerator (9) is equipped with a liquid dispensing port (11) corresponding to the top of the vial (10).

4. The ATP online detection device according to claim 3, characterized in that: The refrigerator (9) is provided with a fixing frame for fixing vials (10). The fixing frame includes a U-shaped frame (12) with its opening facing upwards, which is set inside the refrigerator (9). The two sides of the U-shaped frame (12) are fixed to the inner wall of the refrigerator (9). An n-shaped frame (13) with its opening facing downwards is set inside the U-shaped frame (12). The n-shaped frame (13) is fixed to the U-shaped frame (12). The vial (10) is fixed to the top of the n-shaped frame (13) by a fixing cylinder (14) with its top opening.

5. The ATP online detection device according to claim 4, characterized in that: An annular elastic sleeve is provided on the inner wall of the fixed cylinder (14).

6. The ATP online detection device according to claim 2, characterized in that: The photoelectric detector and the stirrer are connected to a peristaltic pump (17). The reagent bottle (8), the reaction tank (7), the photoelectric detector, and the stirrer are all sampled and extracted through an injection needle (5).

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