Absorption enhancer and application thereof in promoting pesticide absorption and crop growth

Abstract

The invention discloses an absorption enhancer and application thereof in promoting pesticide absorption, and belongs to the technical field of agriculture. The absorption enhancer is obtained by mixing small peptide, amino acid and chitosan oligosaccharide, the absorption enhancer and systemic pesticide are mixed for seed dressing or foliage spraying, the pesticide content in crop plants can be remarkably increased, and the absorption enhancer has important significance in increasing the pesticide utilization rate and reducing soil pollution.

Description

Technical Field

[0001] The present application relates to the technical field of pesticides, and in particular to an absorption enhancer and its application in promoting the absorption of systemic pesticides by crops. Background Art

[0002] Rice seeds are susceptible to a variety of pests and diseases during the germination and field growth stages, resulting in weak seedlings, dead seedlings or reduced yields, endangering rice safety. Chlorantraniliprole (CAP), trifluanid and thiophanate-methyl are three commonly used systemic pesticides that can control pre-seedling pests and diseases such as rice leaf rollers, rice planthoppers and sheath blight, and can also effectively control pests and diseases during the rice growth period, achieving a one-time application and long-term control effect.

[0003] The application methods of these systemic pesticides include seed dressing and foliar spraying. There are still some problems in actual use, such as a large amount of pesticides being adsorbed by the soil but not absorbed by the plant roots, low pesticide utilization rate and easy formation of pesticide resistance, causing soil pollution. Therefore, how to improve the utilization rate of these systemic pesticides has become a technical problem that needs to be solved urgently. Summary of the invention

[0004] In view of the above problems, the present application provides an absorption enhancer that can promote the absorption of pesticides, so as to improve the utilization rate of systemic pesticides and reduce the amount of pesticides used.

[0005] Specifically, the present application achieves the above-mentioned purpose through the following scheme.

[0006] First, the present application provides an absorption enhancer obtained by mixing small peptides, amino acids, and chitosan oligosaccharides. Preferably, in the enhancer, the mass ratio of small peptides, amino acids, and chitosan oligosaccharides is (4-8): (4-8): 1. Further preferably, the small peptides are plant protein small molecule peptides, and the amino acids are plant amino acid complexes.

[0007] Secondly, the present application provides the use of the above absorption enhancer in promoting the absorption of systemic pesticides by crops. The above crops include rice and wheat; the above systemic pesticides include systemic insecticides and systemic fungicides, such as fenvalerate, trifluanid, thiophanate-methyl, prothioconazole, fludioxonil, thiocarbamide, etc.

[0008] Furthermore, the above application refers to mixing the absorption enhancer with crop seeds and then mixing the seeds, or spraying the crop plants, so as to promote the absorption of systemic pesticides by crops; the above seed mixing refers to mixing the seeds with an absorption enhancer with a mass concentration of 40%, or mixing the absorption enhancer with a concentration of 40% with pesticides and then mixing the seeds together, and the absorption enhancer usage is 10g / Kg, so as to improve the absorption of systemic pesticides by crops; the above foliar spraying refers to diluting the absorption enhancer with a mass concentration of 40% by 40 times and spraying it on crop plants together with pesticides to improve the absorption of systemic pesticides by crops.

[0009] Compared with the prior art, the present invention is based on the characteristic that amino acids can promote crop growth, and the absorption enhancer (absorption enhancer) prepared by mixing amino acids with small peptides and chitosan can significantly promote the absorption rate of systemic pesticides by crops, increase the pesticide content in crops, and achieve the purpose of reducing the amount of pesticides used. At the same time, the complex can also promote the growth of crop seedlings, increase the fresh weight of plants and chlorophyll content. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 These are the test results of the three pesticide contents in rice seedlings in Example 1.

[0011] Figure 2 The results of measuring the plant height of rice seedlings in Example 1 are shown in FIG.

[0012] Figure 3 These are the test results of fresh weight and dry weight of rice seedlings in Example 1.

[0013] Figure 4 This is the test result of N content in rice seedlings in Example 1.

[0014] Figure 5 This is the test result of chlorophyll content in rice seedling leaves in Example 1.

[0015] Figure 6 This is a picture of rice seedlings in Example 1;

[0016] Among them, AD represents the growth conditions of rice seedlings in the control group, ratio 1, ratio 2, and ratio 3 groups respectively.

[0017] Figure 7 This is the detection result of thiamethoxam content in rice seedlings in Example 2.

[0018] Figure 8 This is the detection result of the difenoconazole content in rice seedlings in Example 2.

[0019] Fig. 9 This is the detection result of chlorantraniliprole content in rice seedlings in Example 2.

[0020] Fig.10This is the detection result of thiamethoxam content in rice seedlings in Example 3.

[0021] Fig.11 This is the detection result of the difenoconazole content in rice seedlings in Example 3.

[0022] Fig.12 This is the detection result of chlorantraniliprole content in rice seedlings in Example 3.

[0023] Fig.13 These are the test results of the three pesticides, thiamethoxam, prothioconazole and fludioxonil, in rice seedlings in Example 4.

[0024] Fig.14 These are the test results of the contents of two pesticides, prothioconazole and cyclohexane, in rice seedlings in Example 5.

[0025] Fig.15 These are the test results of the three pesticide contents in wheat seedlings in Example 6.

[0026] Fig.16 This is a photo of the growth status of rice seedlings in Example 7;

[0027] Among them, A is the growth status of seedlings in the seedling tray, and B is the growth status of a single seedling.

[0028] Fig.17 The results of the detection of physiological parameters of rice in the tillering stage in Example 7;

[0029] Among them, AE are the test results of rice plant height, root length, chlorophyll content, whole plant biomass, and tiller number, respectively.

[0030] Fig.18 This is a photo of the status of rice plants in the tillering stage of Example 7.

[0031] Fig.19 This is the state of 10-hole rice ears of rice in Example 7. DETAILED DESCRIPTION

[0032] Sources of reagents and raw materials:

[0033] Small peptides are soybean protein small molecule peptides purchased from Beijing Weitailit Biopharmaceutical Co., Ltd.;

[0034] Amino acids were soybean compound amino acids purchased from Ruiyang Biotechnology Company in Mianzhu City, Sichuan Province;

[0035] Chitosan oligosaccharide was purchased from Shanghai Yuanye Biotechnology Co., Ltd.;

[0036] The pesticides involved in the examples were all purchased from DuPont, USA.

[0037] Example 1 Seed dressing experiment with an absorption promoter

[0038] Test rice: Nanjing 9108

[0039] In this embodiment, the absorption enhancer (absorption promoter) is designed in three ratios. When used, it is diluted with water to form an absorption promoter solution with a mass concentration of 40%, and the usage amount is 10g / Kg of seeds.

[0040] Ratio 1: small peptide: amino acid: chitosan oligosaccharide = 8:4:1 (concentration: small peptide 24.62%, amino acid 12.31%, chitosan oligosaccharide 3.08%)

[0041] Ratio 2: small peptide: amino acid: chitosan oligosaccharide = 4:8:1 (concentration: small peptide 12.31%, amino acid 24.62%, chitosan oligosaccharide 3.08%)

[0042] Ratio 3: small peptide: amino acid: chitosan oligosaccharide = 4:4:1 (concentration: small peptide 17.78%, amino acid 17.78%, chitosan oligosaccharide 4.44%)

[0043] Three types of pesticides were used for seed dressing at the same time, and the usage amounts of the three systemic pesticides were:

[0044] 20% (mass percentage) chlorantraniliprole suspension: 5.625ml / Kg seeds

[0045] 10% (mass percentage) triflupromide suspension: 18.75 / Kg seeds

[0046] 24% (mass percentage) Thifluzamide suspension: 2.81 / Kg seeds

[0047] Experimental method: Mix the suction promoter, pesticide and rice seeds, mix the seeds, and then raise the seedlings. Sow 166g of seeds in each seedling tray (60cm×30cm×3.5cm), and 5 seedling trays for each treatment. Samples were taken 21 days after seeding. At the same time, a control group without suction promoter was set up, and the amount of suction promoter added to the suction promoter group was as shown in the above ratio 1-3. There were 4 treatments in total, 5 replicates for each treatment, and the results were averaged.

[0048] The three pesticide residues in the seedlings were tested separately, and the test results are as follows Figure 1As shown. Among them, the determination of chlorantraniliprole content uses the method disclosed in the document "Si Wenshuai, Zhang Ying, Zhang Qicai, et al. Study on LC-MS / MS detection method of fluopyram, enoxamyl and chlorantraniliprole residues in plant-derived foods [J] Shanghai Journal of Agriculture. 2019"; the determination of trifluanid content uses the method disclosed in the document "Guo Yajun, Zhao Ming, Chen Xiaojun, et al. Degradation dynamics and residual analysis of trifluanid in rice fields [J] Jiangsu Agricultural Sciences, 2021, 49(02): 71-5+80."; the determination of thiothiazide content refers to the method disclosed in the document "Duan Tingting, Zhang Ying, Wei Jin, et al. Rapid detection of thiothiazide residues in polished rice, corn and potato samples by ultra-high performance liquid chromatography-tandem mass spectrometry [J] Journal of Analysis and Testing. 2014, 33(05): 598-601."

[0049] The test results of seedling height, weight, N concentration and chlorophyll concentration are as follows Figure 2-5 As shown. The physiological indicators of seedlings refer to the method disclosed in the literature "Huang Xianbo, Yan Han, Hu Jianlin, et al. Changes of several related physiological indicators of rice seedlings under high temperature stress [J] Journal of Yangtze University (Natural Science Edition) Agricultural Science Volume. 2008, 02): 50-3+112." It can be seen that after adding the absorption promoter to the experimental group, the absorption rate of pesticides can be significantly promoted, while promoting the growth of seedlings, and increasing the fresh weight and chlorophyll content.

[0050] Figure 6 These are photos of the growth of rice seedlings in the chlorantraniliprole test, where AD represents the control group, ratio 1, ratio 2, and ratio 3, respectively. It can be seen that the addition of the absorption promoter significantly promoted the growth of the seedlings.

[0051] Example 2

[0052] Test rice: Nanjing 9108

[0053] In this embodiment, the preparation ratio of the absorption promoter, the seed dressing method and the dosage are the same as those in Example 1, and the pesticides are sprayed 14 days after the rice seedlings emerge (three pesticides are sprayed separately), and samples are taken for testing 7 days after spraying. At the same time, a control group is set up in which only the corresponding pesticides are sprayed, and each treatment is repeated 5 times, and the test results are averaged.

[0054] The spraying amount of thiamethoxam is 100% of the recommended dosage in the manual (0.1g / L), and the spraying liquid is 30ml / seedling tray. The spraying amount of difenoconazole is 100% of the recommended dosage in the manual (0.5ml / L), and the spraying liquid is 30ml / seedling tray. The spraying amount of chlorantraniliprole is 100% of the recommended dosage in the manual (0.3ml / L), and the spraying liquid is 30ml / seedling tray.

[0055] The test results of the three pesticides, thiamethoxam, difenoconazole and chlorantraniliprole, were averaged. Figure 7-9As shown, the absorption enhancer group also significantly increased the pesticide content in rice seedlings.

[0056] Example 3

[0057] Test rice: Nanjing 9108

[0058] In this embodiment, the preparation ratio of the absorption promoter is the same as that in Example 1.

[0059] Application method: 14 days after rice seedlings emerge, spray pesticides (three pesticides are sprayed separately) and absorption promoters, and take samples for testing 7 days after spraying.

[0060] Use 1kg of rice seeds to place 6 seedling trays (600mm*300mm), water to keep the soil moist, place in a solar greenhouse and manage normally, watering at regular intervals and in fixed quantities every day.

[0061] The spraying amount of the absorption promoter is as follows: the mass concentration of the absorption promoters of the three ratios is 40%, and they are diluted 40 times before spraying, and the spraying amount is 30 ml / seedling tray.

[0062] The three pesticides were sprayed separately, wherein the spray amount of thiamethoxam was 100% of the recommended dosage in the instructions (0.1 g / L), the spray amount of difenoconazole was 100% of the recommended dosage in the instructions (0.5 ml / L), and the spray amount of chlorantraniliprole was 100% of the recommended dosage in the instructions (0.3 ml / L). The spray volume of the three pesticides was 30 ml / seedling tray.

[0063] At the same time, a control group was set up in which only the corresponding pesticide was sprayed, with 4 trays in each treatment, and the test results were averaged.

[0064] The pesticide content in rice stems and leaves was measured 7 days after spraying. The results of the three groups of pesticide tests were as follows: Figure 10-12 As shown, the results showed that the concentrations of the three pesticides increased significantly after adding the absorption enhancer.

[0065] Example 4

[0066] Test rice: Nanjing 9108

[0067] Absorption promoter: The absorption promoter is prepared in the ratio of small peptide: amino acid: chitosan oligosaccharide = 4:4:1, and then prepared into an absorption promoter solution with a mass concentration of 40% (using concentration: small peptide 17.78%, amino acid 17.78%, chitosan oligosaccharide 4.44%), and the usage is 10g / Kg of seeds.

[0068] Pesticide: Thiamethoxam, Prothioconazole, Fludioxonil (20% + 1% + 2%) 5g / kg seeds

[0069] Application method: Mix the absorption promoter and pesticide together with seeds.

[0070] Rice seedlings were raised after seed dressing, and 166 g of seeds were sown in each seedling tray (60 cm × 30 cm × 3.5 cm), and 5 seedling trays were used for each treatment. A control group without adding an absorption promoter was set up at the same time, and 5 replicates were used for each treatment, and the test results were averaged.

[0071] Samples were taken 21 days after seeding and the concentrations of three pesticides were tested, such as Fig.13 As shown. It can be seen that the pesticide content in the absorption promoter group also increased significantly. The detection methods of prothioconazole and fludioxonil were carried out according to the literature "Determination of the residual degradation dynamics of 6 typical pesticides and 3 metabolites in cucumber by QuEChERS-ultra-high performance liquid chromatography-tandem mass spectrometry" and "Evaluation of Dissipation Behavior, Residues, and Dietary Risk Assessment of Fludioxonil in Cherry via QuEChERS Using HPLC-MS / MS Technique", which are both conventional detection methods in this field.

[0072] Example 5

[0073] Test rice: Nanjing 9108

[0074] Absorption promoter: The absorption promoter is prepared in the ratio of small peptide: amino acid: chitosan oligosaccharide = 4:4:1, and then prepared into a 40% absorption promoter solution (using concentration: small peptide 17.78%, amino acid 17.78%, chitosan oligosaccharide 4.44%), and then diluted 100 times for soaking seeds.

[0075] Pesticide: dilute 400 times of prothioconazole and insecticide ring (2% + 10%) and soak the seeds.

[0076] Application method: Soak the seeds with the absorption promoter and the pesticide together for 48 hours, then raise the seedlings. Sow 166g of seeds in each seedling tray (60cm×30cm×3.5cm), and treat 5 seedling trays. Take samples 21 days after seeding and test the concentrations of the two pesticides. No absorption promoter was added to the control, and each treatment was repeated 5 times, and the test results were averaged.

[0077] The test results of prothioconazole and insecticide ring content are as follows Fig.14 As shown, the contents of the two pesticides in the absorption promoter group increased significantly. The detection methods of prothioconazole and thiocarbamide were respectively carried out according to the literature "Determination of the residual degradation dynamics of 6 typical pesticides and 3 metabolites in cucumber by QuEChERS-ultra-high performance liquid chromatography-tandem mass spectrometry" and "Zhao Xiangmei. Research on the technology of detecting rice pesticide multi-residue by improved QuEChERS method / GC-MS [D]. Jiangsu University, 2008. DOI: 10.7666 / d.y1451465.", which are conventional detection methods in this field.

[0078] Example 6

[0079] Test wheat: Yangmai 33

[0080] Absorption promoter: The absorption promoter is prepared in the ratio of small peptide: amino acid: chitosan oligosaccharide = 4:4:1, and then prepared into a 40% absorption promoter solution (using concentration: small peptide 17.78%, amino acid 17.78%, chitosan oligosaccharide 4.44%), and the usage is 10g / Kg of seeds.

[0081] Pesticide: Thiamethoxam, Prothioconazole, Fludioxonil (20% + 1% + 2%) 5g / kg seeds

[0082] Application method: The absorption promoter and the pesticide were mixed together, and the wheat seeds were seeded after the mixture was added. Each seedling tray (60cm×30cm×3.5cm) was planted with 150g of seeds per seedling tray, and 5 seedling trays were used for each treatment. Samples were taken 21 days after seeding and the concentrations of the three pesticides were measured. At the same time, a control group without the addition of the absorption promoter was set up, and each treatment was repeated 5 times, and the test results were averaged.

[0083] Test results such as Fig.15 As shown, the absorption enhancer group also promoted the contents of three pesticides in wheat plants.

[0084] Example 7

[0085] Test rice: Nanjing 9108

[0086] Demonstration group: The absorption enhancer was prepared according to the mass ratio of small peptide: amino acid: chitosan oligosaccharide = 4:4:1, and then water was added to prepare an absorption enhancer solution with a mass concentration of 40% (using concentration: small peptide 17.78%, amino acid 17.78%, chitosan oligosaccharide 4.44%), and the usage was 10g / Kg of seeds.

[0087] Control group: no suction enhancer added

[0088] Application method: Mix the absorption promoter with seeds without adding pesticides.

[0089] Experimental method: After rice seed dressing, 166g of seeds were sown in each seedling tray (60cm×30cm×3.5cm). A control group without the addition of an absorption promoter was set up. Fig.16 The rice plants were transplanted to the field by machine, and the growth of the plants was observed 33 days after transplanting, and the yield was measured when the rice matured.

[0090] The test results of plant height, root length, chlorophyll concentration, whole plant biomass, and tiller number of rice plants at the tillering stage 33 days after transplanting are as follows: Fig.17 As shown in Figure 2, the growth status of rice plants at this time is as follows: Fig.18As shown in Figure 2, the tillering number and the number of grains per panicle of rice in the rice absorption promoter group increased.

[0091] After maturity, the state of the rice ears in 10 holes is shown Fig.19 The yield test results showed that the control group was 1231 jin / mu, and the demonstration group was 1346 jin / mu, an increase of 9.27%.

[0092] It can be seen that the addition of absorption enhancers can significantly promote the absorption rate of pesticides and increase production at the same time.

Claims

1. An absorption enhancer, characterized in that: The absorption enhancer is obtained by mixing small peptides, amino acids and chitosan oligosaccharides in a mass ratio of (4-8): (4-8):

1.

2. The absorption enhancer according to claim 1, characterized in that The small peptide is a soybean protein small molecule peptide.

3. Use of the absorption enhancer as claimed in claim 1 or 2 in promoting the absorption of systemic pesticides by crops.

4. The use according to claim 3, characterized in that: The systemic pesticides include systemic insecticides and systemic fungicides.

5. The use according to claim 3, characterized in that: The application refers to using the absorption enhancer for seed dressing or foliar spraying to promote the absorption of pesticides by crops.

6. The use according to claim 3, characterized in that: The crop includes rice or wheat.

7. The use according to claim 4, characterized in that: The systemic insecticide includes at least one of chlorantraniliprole, trifluanid and cypermethrin.

8. The use according to claim 4, characterized in that: The systemic fungicide includes at least one of thiophanate-methyl, prothioconazole and fludioxonil.

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