Tobacco bud suppressant and method of making same

Abstract

The application relates to the tobacco cultivation technical field and discloses a tobacco bud inhibition agent and a preparation method thereof. According to the application, the tobacco bud inhibition agent comprises the following components in percentage by mass: 5-20% of indole-3-acetic acid methyl ester, 35-70% of a ketone solvent, 8-20% of an emulsifier and the rest of deionized water. The preparation method of the tobacco bud inhibition agent comprises the following steps: dissolving indole-3-acetic acid methyl ester in the ketone solvent to form a solution. The emulsifier is added into the solution, stirred uniformly and an oil phase is formed. The deionized water is added into the oil phase, stirred according to preset stirring parameters and the tobacco bud inhibition agent is obtained. In the application, the indole-3-acetic acid methyl ester, the ketone solvent, the emulsifier and the deionized water are synergistically combined, the bud can be effectively inhibited, the drug residue can be reduced and the quality of tobacco leaves is improved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of tobacco cultivation, and particularly relates to a tobacco bud inhibition agent and a preparation method thereof. BACKGROUND

[0002] The types of tobacco bud inhibition agents include chemical bud inhibition agents, such as brassinolide, calcium chloride, ethephon, and the like.

[0003] In actual application, although the chemical bud inhibition agent can inhibit the growth of tobacco buds, it has the problem of drug residue, which affects the quality of tobacco leaves. Moreover, long-term use of the chemical bud inhibition agent can also cause environmental pollution. SUMMARY

[0004] The present application aims to at least solve one of the technical problems in the related art.

[0005] Therefore, a first aspect of the present application provides a tobacco bud inhibition agent.

[0006] A second aspect of the present application provides a preparation method of the tobacco bud inhibition agent.

[0007] Therefore, a first aspect of the present application provides a tobacco bud inhibition agent.

[0008] Optionally, the ketone solvent includes one or more of acetone, isoforone, and cyclohexanone.

[0009] Optionally, the emulsifier includes one or more of Farm Emulsifier No. 400, Farm Emulsifier No. 500, Farm Emulsifier No. 600, and Farm Emulsifier No. 1602.

[0010] Optionally, the tobacco bud inhibition agent further includes, by mass percentage, the following components: a co-surfactant 1% to 5%, and / or, an antifreeze agent 1% to 5%.

[0011] Optionally, the co-surfactant includes one or more of ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, 1-hexanol, 2-hexanol, 1-octanol, and 2-octanol.

[0012] Optionally, the antifreeze agent includes one or more of ethylene glycol, propylene glycol, glycerol, and polyethylene glycol.

[0013] According to a second aspect of the embodiments of the present application, a preparation method of the tobacco bud inhibiting agent is provided for preparing the tobacco bud inhibiting agent according to any one of the preceding embodiments, and the preparation method comprises the following steps: dissolving the indole-3-acetic acid methyl ester in the ketone solvent to form a solution; adding the emulsifier into the solution and stirring uniformly to form an oil phase; and adding the deionized water into the oil phase and stirring according to preset stirring parameters to obtain the tobacco bud inhibiting agent.

[0014] Optionally, the preset stirring parameters comprise: a stirring speed of 10,000 r / min to 12,000 r / min; and / or a stirring time of 1 min to 3 min.

[0015] Optionally, in the step of adding the emulsifier into the solution, a co-surfactant is also added into the solution.

[0016] Optionally, in the step of adding the deionized water into the oil phase, an antifreezing agent is also added into the oil phase.

[0017] The tobacco bud inhibiting agent and the preparation method thereof provided by the present application can at least achieve the following technical effects:

[0018] In the present application, the indole-3-acetic acid methyl ester can effectively inhibit the growth of buds. The indole-3-acetic acid methyl ester has biological activity and can be metabolized by plants to reduce residues. The ketone solvent can achieve volatilization and degradation, which helps to reduce pesticide residues. The emulsifier and the deionized water help the tobacco bud inhibiting agent to be uniformly distributed on the surface of the tobacco leaves, reducing residues caused by local overuse. In the present application, through the synergistic effect of the indole-3-acetic acid methyl ester, the ketone solvent, the emulsifier and the deionized water, effective bud inhibition can be achieved, drug residues can be reduced, and the quality of tobacco leaves can be improved.

[0019] The foregoing general description and the following description are only exemplary and explanatory, and are not used to limit the present application. BRIEF DESCRIPTION OF DRAWINGS

[0020] One or more embodiments are exemplarily illustrated by the corresponding drawings, which do not constitute a limitation on the embodiments, and the elements with the same reference numerals in the drawings are shown as similar elements, the drawings do not constitute a proportional limitation, and wherein:

[0021] Figure 1 A flow chart of the preparation method of the tobacco bud inhibiting agent provided for one embodiment of the present disclosure;

[0022] Figure 2 A flow chart of the preparation method of the tobacco bud inhibiting agent provided for another embodiment of the present disclosure;

[0023] Figure 3The high performance liquid chromatogram of the tobacco bud suppressant sample before the heat storage experiment provided for Embodiment 1 of the present disclosure;

[0024] Figure 4 The high performance liquid chromatogram of the tobacco bud suppressant sample after the heat storage experiment provided for Embodiment 1 of the present disclosure. DETAILED DESCRIPTION

[0025] In order to enable a more detailed understanding of the features and technical content of the embodiments of the present disclosure, the implementation of the embodiments of the present disclosure will be described in detail below with reference to the accompanying drawings, which are only used for reference and do not limit the embodiments of the present disclosure. In the following technical description, in order to facilitate explanation, a plurality of details are provided to provide a full understanding of the disclosed embodiments. However, one or more embodiments can still be implemented without these details.

[0026] Unless otherwise specified, the term "a plurality of" means two or more.

[0027] In the embodiments of the present disclosure, the character " / " represents an "or" relationship between the objects before and after it. For example, A / B represents: A or B.

[0028] The term "and / or" is a description of the association between objects, which means that there can be three relationships. For example, A and / or B, which means: A or B, or, A and B, three relationships.

[0029] It should be noted that the embodiments in the embodiments of the present disclosure and the features in the embodiments can be combined with each other without conflict.

[0030] It should be noted that the term "emulsion in water (EW)" used in the embodiments of the present disclosure, also known as concentrated emulsion, is a solution that is not soluble in water obtained by dissolving liquid or solid original medicine that is not soluble in water in an organic solvent, dispersed in water, and formed. An environmentally friendly pesticide preparation. Additives and process technology to make the oil phase uniformly distributed in the water phase to form a stable emulsion in water system. The oil bead size of the emulsion in water is usually 0.7-20 μm, and the ideal size is 1.5-3.5 μm.

[0031] It should be noted that the cold storage experiment used in the embodiments of the present disclosure is used to evaluate the cold storage stability. The specific method is: take 5 ml sample in a 10 ml test tube with a plug, and place it in a refrigerator with a temperature of (0±1) ℃, and store for 7 days. If the sample can maintain a uniform state, there is no oil on the top, and there is no sediment at the bottom, the cold storage stability is qualified.

[0032] It should be noted that the heat storage experiment used in the embodiments of the present disclosure is used to evaluate the heat storage stability. The specific method is as follows: 5 ml sample is taken in a 10 ml test tube with a plug, and is placed in a constant temperature oven with a temperature of (54±2) °C for storage for 14 days. If the appearance of the sample remains uniform and transparent, the heat storage stability is qualified. If a small amount of stratification appears, it can be restored to its original state after slight shaking, and it is also considered that the heat storage stability is qualified.

[0033] It should be noted that the normal temperature storage experiment used in the embodiments of the present disclosure is used to evaluate the normal temperature stability. The specific method is as follows: 5 ml sample is taken in a 10 ml test tube with a plug, and is stored at room temperature in the dark. If the appearance of the sample is uniform, the normal temperature stability is qualified.

[0034] It should be noted that the emulsion stability test used in the embodiments of the present disclosure is used to evaluate the emulsion stability. The emulsion stability refers to the stability of the emulsion formed after the water emulsion is diluted with water. The emulsion stability test method is performed according to GB / T1603-2001. In a 250 mL beaker, 100 mL of standard hard water with a temperature of 25-30 °C is added, and 0.5 mL of water emulsion sample (diluted 200 times) is taken with a pipette. Under continuous stirring, the standard hard water is slowly added, and after the water emulsion is added, the stirring is continued at a speed of 2-3 r / s for 30 s. The emulsion is immediately transferred to a clean and dry 100 mL graduated cylinder, and the graduated cylinder is placed in a constant temperature water bath at (30±2) °C, and is left to stand for 1 h. The separation of the emulsion is observed. If there is no oil floating, precipitation and oil separation in the graduated cylinder, the emulsion stability is qualified.

[0035] It should be noted that the evaluation standard of emulsion dispersibility used in the embodiments of the present disclosure is: according to the determination method of emulsion stability in national standard GB / T1603-2001, under the condition of 25±1℃, 1 mL of the preparation sample is taken at a distance of 2 cm from the water surface, and is slowly added into a beaker containing 200 mL of standard hard water, and is diluted and observed. The dispersion state of the oil phase (preparation sample) in water, the emulsification state and the evaluation level are shown in Table 1. That is, as shown in Table 1, when the level of the emulsion dispersibility of the preparation sample is 1, it is marked as “+++”, the dispersion state is that it can be rapidly and automatically uniformly dispersed, and the emulsification state is that it is a blue or milky white transparent emulsion after slight stirring. When the level of the emulsion dispersibility of the preparation sample is 2, it is marked as “++”, the dispersion state is that it can be automatically uniformly dispersed, and the emulsification state is that it is a blue translucent emulsion after slight stirring. When the level of the emulsion dispersibility of the preparation sample is 3, it is marked as “+”, the dispersion state is white cloud or silk-like dispersion, and the emulsification state is a blue opaque emulsion after stirring. When the level of the emulsion dispersibility of the preparation sample is 4, it is marked as “-”, the dispersion state is white particulate sinking, and the emulsification state is a white-blue opaque emulsion after stirring. When the level of the emulsion dispersibility of the preparation sample is 5, it is marked as “--”, the dispersion state is oil bead sinking, and the emulsification state is that it can be emulsified after stirring, and quickly separates after stopping stirring.

[0036] Table 1 Dispersion state of oil phase in water, emulsification state and evaluation level

[0037]

[0038] The embodiments of the present disclosure provide a tobacco bud inhibitor. The tobacco bud inhibitor comprises, by mass percentage, the following components: indole-3-acetic acid methyl ester 5% to 20%, ketone solvent 35% to 70%, emulsifier 8% to 20%, and the rest is deionized water.

[0039] Specifically, in the tobacco bud inhibitor, the mass of indole-3-acetic acid methyl ester accounts for 5% to 20% of the total mass of the tobacco bud inhibitor. The mass of the ketone solvent accounts for 35% to 70% of the total mass of the tobacco bud inhibitor. The mass of the emulsifier accounts for 8% to 20% of the total mass of the tobacco bud inhibitor, and the rest is deionized water. That is, in the tobacco bud inhibitor, the content of indole-3-acetic acid methyl ester is 5% to 20%, the content of the ketone solvent is 35% to 70%, the content of the emulsifier is 8% to 20%, and the rest is deionized water.

[0040] In the embodiments, the tobacco bud inhibitor comprises indole-3-acetic acid methyl ester, ketone solvent, emulsifier and deionized water. Among them, after indole-3-acetic acid methyl ester is dissolved in the ketone solvent, the emulsifier is added to form an oil phase. The deionized water is used to provide an aqueous phase. Through the oil phase and the aqueous phase, the dosage form of the tobacco bud inhibitor can be a water emulsion, thereby realizing the safety, environmental protection, economy and high efficiency of the tobacco bud inhibitor.

[0041] In this embodiment, the bud growth can be effectively inhibited by indole-3-acetic acid methyl ester. Indole-3-acetic acid methyl ester belongs to auxin compounds, has biological activity, can be metabolized by plants, and reduces residues. Ketone solvents can achieve volatilization and degradation, which helps to reduce pesticide residues. Emulsifiers and deionized water help tobacco bud inhibitors to be evenly distributed on the surface of tobacco leaves, reducing residues caused by local excess. Through the synergistic effect of indole-3-acetic acid methyl ester, ketone solvents, emulsifiers and deionized water, effective bud inhibition can be achieved, drug residues can be reduced, and the quality of tobacco leaves can be improved.

[0042] When applied to tobacco plants, the emulsion type tobacco bud inhibitor can be evenly distributed on the surface of the tobacco plants, achieving effective coverage of the agent and improving the bud inhibition effect. The combination of the water phase and the oil phase in the tobacco bud inhibitor makes it have good adhesion and permeability, which can act more deeply on the bud points and improve the durability and stability of bud inhibition. The water phase in the tobacco bud inhibitor can also reduce the amount of organic solvent used, reducing costs. The tobacco bud inhibitor also has good biocompatibility. Through the biological activity of indole-3-acetic acid methyl ester, residues can be reduced, which helps to achieve green production of tobacco.

[0043] It can be understood that the tobacco bud inhibitor of the present embodiment can also be used for other plants to achieve effective bud inhibition while reducing drug residues.

[0044] Optionally, in the tobacco bud inhibitor, the content of indole-3-acetic acid methyl ester is 5%, 10%, 15%, 20%, or other values in the range of 5% to 20%.

[0045] Optionally, in the tobacco bud inhibitor, the content of ketone solvent is 35%, 40%, 50%, 60%, 70%, or other values in the range of 35% to 70%.

[0046] Optionally, in the tobacco bud inhibitor, the content of the emulsifier is 8%, 12%, 15%, 20%, or other values in the range of 8% to 20%.

[0047] Indole-3-acetic acid methyl ester is an example of a product of the methylation of indole-3-acetic acid by the indole-3-acetic acid carboxyl methyltransferase 1 (IAMT1) gene. Indole-3-acetic acid methyl ester can freely enter and exit cells and is another form of indole-3-acetic acid that plants use to regulate the activity of indole-3-acetic acid in plants. In previous work, the present inventors isolated a strain of Kluyvera ascorbata, ZLSY22, from the digestive tract of the Eupatorium adenophorum parasitic insect Procecidochares utilis Stone. The fermentation broth of the strain can inhibit the germination of seeds of Eupatorium adenophorum and other plants. From the precipitate and supernatant of the fermentation broth of ZLSY22, the present inventors isolated 41 compounds. Using seed germination and seedling cup methods, the present inventors screened four compounds with herbicidal activity, of which indole-3-acetic acid methyl ester had a better inhibitory effect on Eupatorium adenophorum and its seedlings.

[0048] In some embodiments, the ketone solvent includes one or more of acetone, isoforone, and cyclohexanone.

[0049] In this embodiment, the ketone solvent includes one or more of acetone, isoforone, and cyclohexanone, which has a good solubility for the original drug (indole-3-acetic acid methyl ester). The ketone solvent can be volatilized and degraded, which helps to reduce pesticide residues. The ketone solvent has stable physical and chemical properties, and the ketone solvent together with the emulsifier can reduce the surface tension and increase the flowability.

[0050] As an example, the present inventors used indole-3-acetic acid methyl ester in solid form to observe the solubility of indole-3-acetic acid methyl ester (solute) in acetone, isoforone, and cyclohexanone, respectively, and the results are shown in Table 2.

[0051] Table 2 Solubility of indole-3-acetic acid methyl ester in ketone solvents

[0052] Solvent Solute / Solvent (g / mL) Dissolution State Cold Storage Stability Quality Acceptance Acetone 1 / 2 Dissolved Acceptable Acceptable Isoflurodone 1 / 3 Dissolved Acceptable Acceptable Cyclohexanone 1 / 2 Dissolved Acceptable Acceptable

[0053] As can be seen from Table 2, at room temperature, indole-3-acetic acid methyl ester can be dissolved in acetone with a small amount of solute, and has a good solubility. Moreover, at (0±1)℃, the original drug (indole-3-acetic acid methyl ester) does not precipitate, and can maintain a uniform state without floating oil on the top and precipitate on the bottom, and has qualified cold storage stability. Therefore, the quality is qualified.

[0054] As can be seen from Table 2, at room temperature, indole-3-acetic acid methyl ester can be dissolved in isoforone with a small amount of solute, and has a good solubility. Moreover, at (0±1)℃, the original drug (indole-3-acetic acid methyl ester) does not precipitate, and can maintain a uniform state without floating oil on the top and precipitate on the bottom, and has qualified cold storage stability. Therefore, the quality is qualified.

[0055] From Table 2, it can be obtained that, at room temperature, indole-3-acetic acid methyl ester can be dissolved in cyclohexanone, and the amount of solute is small, and the dissolution effect is good. And, at (0±1)℃, the original drug (indole-3-acetic acid methyl ester) does not precipitate, and can maintain a uniform state, without floating oil on the top and without precipitation on the bottom, and the cold storage stability is qualified. Therefore, the quality qualification degree is qualified.

[0056] In some embodiments, the emulsifier includes one or more of Farm Emulsifier No. 400, Farm Emulsifier No. 500, Farm Emulsifier No. 600, and Farm Emulsifier No. 1602.

[0057] In this embodiment, the emulsifier includes one or more of Farm Emulsifier No. 400, Farm Emulsifier No. 500, Farm Emulsifier No. 600, and Farm Emulsifier No. 1602, which is combined with the ketone solvent to reduce the surface tension and increase the flowability. The emulsifier is combined with deionized water to help the tobacco bud inhibitor evenly distribute on the surface of the tobacco leaf and reduce the residue caused by local excess.

[0058] In this embodiment, Farm Emulsifier No. 400, Farm Emulsifier No. 500, Farm Emulsifier No. 600, and Farm Emulsifier No. 1602 have good emulsification effect, which helps to improve the bud inhibition effect of the tobacco bud inhibitor and reduce the drug residue effect.

[0059] For example, normal temperature storage experiment, heat storage experiment and emulsion dispersibility evaluation are respectively performed on Farm Emulsifier No. 400, Farm Emulsifier No. 500, Farm Emulsifier No. 600, and Farm Emulsifier No. 1602 to evaluate the emulsification effect. The results are shown in Table 3.

[0060] Table 3 Emulsification effect of emulsifier

[0061]

[0062] In this example, from Table 3, it can be obtained that the normal temperature stability of Farm Emulsifier No. 400 is qualified, the heat storage stability is qualified, and the emulsion dispersibility level is level 1, therefore, the quality qualification degree is qualified. The normal temperature stability of Farm Emulsifier No. 500 is qualified, the heat storage stability is qualified, and the emulsion dispersibility level is level 2, therefore, the quality qualification degree is qualified. The normal temperature stability of Farm Emulsifier No. 600 is qualified, the heat storage stability is qualified, and the emulsion dispersibility level is level 1, therefore, the quality qualification degree is qualified. The normal temperature stability of Farm Emulsifier No. 1602 is qualified, the heat storage stability is qualified, and the emulsion dispersibility level is level 2, therefore, the quality qualification degree is qualified. That is, the emulsification effect of Farm Emulsifier No. 400, Farm Emulsifier No. 500, Farm Emulsifier No. 600, and Farm Emulsifier No. 1602 is good, which helps to improve the bud inhibition effect of the tobacco bud inhibitor and reduce the drug residue effect.

[0063] As a comparative example, the emulsifying effects of emulsifiers NP-10, OP-10, Tween 80, Tween 20, Span 80 and Span 20 were evaluated by normal-temperature storage experiments, heat storage experiments and emulsion dispersibility evaluation. The results are shown in Table 3.

[0064] In the present comparative example, it can be seen from Table 3 that the normal-temperature stability of emulsifier NP-10 was unqualified (delamination), the heat storage stability was unqualified (delamination), and the emulsion dispersibility was rated as level 5, so the quality qualification was unqualified. The normal-temperature stability of emulsifier OP-10 was unqualified (delamination), the heat storage stability was unqualified (delamination), and the emulsion dispersibility was rated as level 5, so the quality qualification was unqualified. The normal-temperature stability of Tween 80 was unqualified (delamination), the heat storage stability was unqualified (delamination), and the emulsion dispersibility was rated as level 3, so the quality qualification was unqualified. The normal-temperature stability of Tween 20 was qualified, but the heat storage stability was unqualified (delamination), and the emulsion dispersibility was rated as level 5, so the quality qualification was unqualified. The normal-temperature stability of Span 80 was qualified, but the heat storage stability was unqualified (oil separation), and the emulsion dispersibility was rated as level 3, so the quality qualification was unqualified. The normal-temperature stability of Span 20 was unqualified (delamination), the heat storage stability was unqualified (crystallization), and the emulsion dispersibility was rated as level 5, so the quality qualification was unqualified. That is, if emulsifiers NP-10, OP-10, Tween 80, Tween 20, Span 80 and Span 20 in the comparative example are applied to tobacco bud inhibitors, the bud inhibition effect and the drug residue reduction effect in the present application cannot be achieved.

[0065] It can be understood that when performing normal-temperature storage experiments, heat storage experiments and emulsion dispersibility evaluation, each emulsifier can be subjected to multiple experiments, for example, 3 experiments, to achieve accurate evaluation of the emulsifying effect.

[0066] Alternatively, the emulsifier comprises Nonglu No. 400.

[0067] Alternatively, the emulsifier comprises Nonglu No. 500.

[0068] Alternatively, the emulsifier comprises Nonglu No. 600.

[0069] Alternatively, the emulsifier comprises Nonglu No. 1602.

[0070] Alternatively, the emulsifier comprises Nonglu No. 500 and Nonglu No. 600. The mass ratio of Nonglu No. 600 to Nonglu No. 500 is 1:2.

[0071] Alternatively, the emulsifier comprises Nonglu No. 500 and Nonglu No. 1602. The mass ratio of Nonglu No. 1602 to Nonglu No. 500 is 1:1.

[0072] Optionally, the emulsifier comprises AGRIMUL 400 and AGRIMUL 600. The mass ratio of AGRIMUL 600 to AGRIMUL 400 is 1:1. Alternatively, the mass ratio of AGRIMUL 600 to AGRIMUL 400 is 2:1. Alternatively, the mass ratio of AGRIMUL 600 to AGRIMUL 400 is 1:2.

[0073] In some embodiments, the tobacco bud inhibitor further comprises, by mass percentage, the following components: a co-surfactant 1% to 5%, and / or an antifreezing agent 1% to 5%.

[0074] In this embodiment, the co-surfactant is used to change the hydrophilic-lipophilic property of the emulsifier, so that the tobacco bud inhibitor is more stable. The antifreezing agent is used to prevent or reduce freezing at low temperatures.

[0075] Optionally, the tobacco bud inhibitor comprises, by mass percentage, the following components: indole-3-acetic acid methyl ester 5% to 20%, a ketone solvent 35% to 70%, an emulsifier 8% to 20%, a co-surfactant 1% to 5%, and the rest is deionized water.

[0076] Optionally, the tobacco bud inhibitor comprises, by mass percentage, the following components: indole-3-acetic acid methyl ester 5% to 20%, a ketone solvent 35% to 70%, an emulsifier 8% to 20%, an antifreezing agent 1% to 5%, and the rest is deionized water.

[0077] Optionally, the tobacco bud inhibitor comprises, by mass percentage, the following components: indole-3-acetic acid methyl ester 5% to 20%, a ketone solvent 35% to 70%, an emulsifier 8% to 20%, a co-surfactant 1% to 5%, an antifreezing agent 1% to 5%, and the rest is deionized water.

[0078] Optionally, the content of the co-surfactant in the tobacco bud inhibitor is 1%, 2%, 3%, 4%, 5%, or other values within the range of 1% to 5%.

[0079] Optionally, the content of the antifreezing agent in the tobacco bud inhibitor is 1%, 2%, 3%, 4%, 5%, or other values within the range of 1% to 5%.

[0080] In some embodiments, the co-surfactant comprises one or more of ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, 1-hexanol, 2-hexanol, 1-octanol, and 2-octanol.

[0081] In this embodiment, the co-surfactant comprises one or more of ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, 1-hexanol, 2-hexanol, 1-octanol, and 2-octanol, which helps to improve the bud inhibition effect of the tobacco bud inhibitor, reduce drug residues, and reduce costs.

[0082] In some embodiments, the antifreeze agent comprises one or more of ethylene glycol, propylene glycol, glycerol, and polyethylene glycol.

[0083] In some embodiments, the antifreeze agent comprises one or more of ethylene glycol, propylene glycol, glycerol, and polyethylene glycol, which helps achieve the low-temperature stability and effectiveness of the tobacco bud inhibitor.

[0084] As shown in Figure 1 The present disclosure also provides a preparation method of the tobacco bud inhibitor, for preparing the tobacco bud inhibitor according to any one of the preceding embodiments. The preparation method comprises the following steps:

[0085] S101, dissolving methyl indole-3-acetate in a ketone solvent to form a solution.

[0086] In this embodiment, by dissolving methyl indole-3-acetate in a ketone solvent to form a solution, methyl indole-3-acetate is uniformly dispersed in the ketone solvent, preparing for the subsequent addition of an emulsifier.

[0087] For example, methyl indole-3-acetate (the original drug) is a solid. Dissolving methyl indole-3-acetate in a ketone solvent to form a solution makes methyl indole-3-acetate uniformly dispersed in the ketone solvent.

[0088] S102, adding an emulsifier to the solution and stirring uniformly to form an oil phase.

[0089] In this embodiment, the emulsifier is added to the solution and stirred uniformly to form an oil phase, which is used for uniform mixing with an aqueous phase. The emulsifier can be used to reduce the surface tension between oil and water, improve the stability and effectiveness of the tobacco bud inhibitor.

[0090] S103, adding deionized water to the oil phase and stirring according to a preset stirring parameter to obtain the tobacco bud inhibitor.

[0091] In this embodiment, deionized water is used to provide an aqueous phase. Stirring according to a preset stirring parameter makes the oil phase and the aqueous phase fully mixed to form a stable emulsion, i.e., a water emulsion type tobacco bud inhibitor, improving the uniformity and effectiveness of the tobacco bud inhibitor.

[0092] In this embodiment, the preparation method has a simple process and low cost. The prepared tobacco bud inhibitor has a long storage period and stability, can effectively inhibit buds, reduce drug residues, improve the quality of tobacco leaves, and is suitable for large-scale production and application.

[0093] In this embodiment, the step of stirring according to a preset stirring parameter can be realized by a high-speed shearing machine.

[0094] In some embodiments, the preset stirring parameters include: stirring speed: 10000 r / min to 12000 r / min; and / or, stirring time: 1 min to 3 min.

[0095] In this embodiment, the aqueous phase and oil phase are fully mixed by stirring at a speed of 10,000 r / min to 12,000 r / min to form a water-emulsion tobacco sprout inhibitor.

[0096] In this embodiment, the water phase and oil phase are fully mixed by stirring for 1 to 3 minutes to form a water-emulsion tobacco sprout inhibitor.

[0097] Optionally, the stirring speed is 10,000 r / min, 11,000 r / min, 12,000 r / min or other values ​​between 10,000 r / min and 12,000 r / min.

[0098] Alternatively, the stirring time may be 1 min, 2 min, 3 min, or other values ​​between 1 min and 3 min.

[0099] In some embodiments, in the step of adding the emulsifier to the solution, a co-surfactant is also added to the solution; and / or, in the step of adding deionized water to the oil phase, an antifreeze is also added to the oil phase.

[0100] In this embodiment, in the step of adding the emulsifier to the solution, a co-surfactant is also added to the solution to improve the sprout-suppressing effect of the tobacco sprout inhibitor and reduce pesticide residues. It is understood that the specific method of adding the co-surfactant to the solution in the step of adding the emulsifier is not limited. For example, the co-surfactant can be added to the solution together with the emulsifier, and then stirred. Another example is that the emulsifier can be added to the solution first, and then stirred, with the co-surfactant added during the stirring process.

[0101] In this embodiment, in the step of adding deionized water to the oil phase, an antifreeze agent is also added to the oil phase to achieve low-temperature stability and effectiveness of the tobacco bud inhibitor. It is understood that the specific method of adding the antifreeze agent to the oil phase in the step of adding deionized water is not limited. For example, deionized water and antifreeze agent can be added to the solution together and then stirred. Another example is that deionized water can be added to the solution first, then stirred, and the antifreeze agent can be added during the stirring process. Yet another example is that the oil phase is stirred, and deionized water and antifreeze agent are added during the stirring process.

[0102] Optionally, such as Figure 2 As shown in the embodiments of this disclosure, a method for preparing a tobacco bud-inhibiting agent is also provided, for preparing the tobacco bud-inhibiting agent as described in any of the preceding embodiments. The preparation method includes the following steps:

[0103] S201, dissolve indole-3-acetic acid methyl ester in a ketone solvent to form a solution.

[0104] S202, add an emulsifier and a co-surfactant to the solution and stir until uniform to form an oil phase.

[0105] S203, add deionized water and an antifreeze agent to the oil phase and stir according to predetermined stirring parameters to obtain a tobacco bud inhibitor.

[0106] The following specific embodiments are given to further illustrate the embodiments of the present disclosure.

[0107] Embodiment 1

[0108] The tobacco bud inhibitor in the present embodiment includes the following components in terms of mass percentage: indole-3-acetic acid methyl ester 10%, acetone solvent 50%, emulsifier 15%, n-butanol 4%, ethylene glycol 4%, and deionized water 17%. The emulsifier includes Farm Emulsifier No. 500 and Farm Emulsifier No. 1602, and the mass ratio of Farm Emulsifier No. 1602 to Farm Emulsifier No. 500 is 1:1.

[0109] The preparation method of the tobacco bud inhibitor in the present embodiment includes the following steps: dissolving indole-3-acetic acid methyl ester in acetone to form a solution. Add an emulsifier and n-butanol to the solution and stir until uniform to form an oil phase. Under the action of a high-speed shearing machine, add deionized water and ethylene glycol to the oil phase, wherein the stirring speed is 10,000 r / min and the stirring time is 3 min to obtain a tobacco bud inhibitor.

[0110] The tobacco bud inhibitor of the present embodiment can effectively inhibit bud. The synergistic effect of indole-3-acetic acid methyl ester, acetone solvent, emulsifier, n-butanol, ethylene glycol, and deionized water can reduce drug residues and improve the quality of tobacco leaves.

[0111] The tobacco bud inhibiting agent of the present embodiment is qualified in appearance stability, emulsion stability, heat storage stability, low temperature stability, pH value, and emulsion dispersibility. Specifically, whether it is a 2-year normal temperature storage experiment or an accelerated storage experiment, the appearance of the tobacco bud inhibiting agent is a clear and transparent liquid without delamination, no oil floating and no precipitation (or crystallization) separation, i.e., the appearance stability is qualified. The emulsion stability test is qualified without oil floating, precipitation and oil separation. The heat storage experiment is qualified without delamination and oil separation, maintaining a good emulsion state, only separating emulsion and water, and still forming a uniform emulsion after gentle shaking. The low temperature stability is qualified without delamination and crystallization after storing in a refrigerator at 0°C, -5°C or -9°C for 1 week or 2 weeks. The pH value of the pesticide is qualified in the range of 4 to 7. The pH value of the tobacco bud inhibiting agent is 6.73, i.e., the pH value is qualified. The emulsion dispersibility is qualified with a grade of 2.

[0112] According to the standard established by the Food and Agriculture Organization of the United Nations (FAO), the storage stability test is performed on the tobacco bud inhibiting agent, and the storage decomposition rate of indole-3-acetic acid methyl ester in the tobacco bud inhibiting agent should be ≤5%. Specifically, the tobacco bud inhibiting agent sample is chemically analyzed by a high performance liquid chromatograph (HPLC) instrument. The HPLC determination conditions are as follows: mobile phase: water: methanol (V / V) = 34:66, degassed filtration; flow rate: 3.0 mL / min; column temperature: 25°C; ultraviolet detection wavelength: 230 nm; injection volume: 10 μL; chromatographic column type: C18 5 μL, 10 nm x 150 mm 130 A; retention time: 5.2 min.

[0113] The tobacco bud inhibiting agent sample before the heat storage experiment is analyzed by the HPLC instrument, and the high performance liquid chromatogram is as shown in Figure 3 , wherein the abscissa represents time (unit: min), and the ordinate represents the response value of the electric signal (unit: mAu). Figure 3 Figure 3 The peak area (unit: mAu·min) of A in is used to illustrate the peak area of indole-3-acetic acid methyl ester of the tobacco bud inhibiting agent sample before the heat storage experiment, which is 67083.45590.

[0114] Figure 4 The tobacco bud inhibiting agent sample after the heat storage experiment is analyzed by the HPLC instrument, and the high performance liquid chromatogram is as shown in Figure 4 , wherein the abscissa represents time (unit: min), and the ordinate represents the response value of the electric signal (unit: mAu). Figure 4 ​The middle B is used to indicate the peak area (unit: mAu·min), and the peak area of indole-3-acetic acid methyl ester of the tobacco bud inhibitor sample after the heat storage experiment is 64854.75008.

[0115] The storage decomposition rate of indole-3-acetic acid methyl ester in the tobacco bud inhibitor is calculated as follows:

[0116] (67083.45590-64854.75008) / 67083.45590×100%=3.3%

[0117] That is, the storage decomposition rate (3.3%) of indole-3-acetic acid methyl ester in the tobacco bud inhibitor in this embodiment is less than 5%, and the storage stability test is qualified.

[0118] The 400-fold, 200-fold and 100-fold solutions of the tobacco bud inhibitor in this embodiment, flumetralin (CK1) and dimethenamid (CK2) are respectively applied to the axillary buds of tobacco (cup drenching method can be used for application), and the bud inhibition effect is shown in Table 4.

[0119] Table 4 Bud inhibition rate and bud inhibition effect

[0120]

[0121] As shown in Table 4, T1 represents the 400-fold solution of the tobacco bud inhibitor. T2 represents the 200-fold solution of the tobacco bud inhibitor. T3 represents the 100-fold solution of the tobacco bud inhibitor. CK1 represents the 200-fold solution of 25% flumetralin emulsion. CK2 represents the 200-fold solution of 33% dimethenamid emulsion.

[0122] As shown in Table 4, the bud inhibition rate and bud inhibition effect data are mean ± standard error. By New Complex Difference Method (Duncan's New Complex Difference Method), in the same column of Table 4, at the 0.05 level, the data marked a is significantly different from the data marked b and c, and is not significantly different from the data marked ab; the data marked b is significantly different from the data marked a and c, and is not significantly different from the data marked ab; the data marked c is significantly different from the data marked a, b and ab; the data marked ab is significantly different from the data marked c, and is not significantly different from the data marked a and b.

[0123] As shown in Table 4, the number of live tobacco buds after 14 days, 28 days and 42 days of application is counted, and the bud inhibition rate and bud inhibition effect of T1, T2, T3, CK1 and CK2 are obtained. The bud inhibition rate can be calculated by the number of live buds more than 2 cm, and the bud inhibition effect can be calculated by the weight of the buds. The bud inhibition rate and bud inhibition effect are as follows:

[0124] 14 days after application, the bud inhibition rates of T1, T2 and T3 were all 100.00%. The bud inhibition rates of CK1 and CK2 were 88.75% and 82.32% respectively. It can be seen that the bud inhibition rates of T1, T2 and T3 were all higher than those of CK1 and CK2. In the same column of Table 4, the data marked with a was not significantly different from the data marked with a, the data marked with a was significantly different from the data marked with b, and the data marked with ab was not significantly different from the data marked with b.

[0125] 28 days after application, the bud inhibition rates of T1, T2 and T3 were 96.80%, 98.60% and 87.94% respectively, and the bud inhibition rates of CK1 and CK2 were 96.27% and 76.21% respectively. It can be seen that the bud inhibition rates of T1 and T2 were both higher than that of CK1. The bud inhibition rates of T1, T2 and T3 were all higher than that of CK2. In the same column of Table 4, the data marked with a was not significantly different from the data marked with a, and the data marked with a was significantly different from the data marked with c.

[0126] 42 days after application, the bud inhibition rates of T1, T2 and T3 were 97.05%, 96.53% and 81.83% respectively. The bud inhibition rates of CK1 and CK2 were 88.19% and 61.61% respectively. It can be seen that the bud inhibition rates of T1 and T2 were both higher than that of CK1. The bud inhibition rates of T1, T2 and T3 were all higher than that of CK2. In the same column of Table 4, the data marked with a was not significantly different from the data marked with a or the data marked with ab, the data marked with a was significantly different from the data marked with b or the data marked with c, the data marked with b was not significantly different from the data marked with ab, and the data marked with c was significantly different from the data marked with ab.

[0127] 42 days after application, the bud inhibition rates of T1, T2 and T3 were 97.05%, 96.53% and 81.83% respectively. The bud inhibition rates of CK1 and CK2 were 88.19% and 61.61% respectively. It can be seen that the bud inhibition rates of T1 and T2 were both higher than that of CK1. The bud inhibition rates of T1, T2 and T3 were all higher than that of CK2. In the same column of Table 4, the data marked with a was not significantly different from the data marked with a or the data marked with ab, the data marked with a was significantly different from the data marked with b or the data marked with c, the data marked with b was not significantly different from the data marked with ab, and the data marked with c was significantly different from the data marked with ab.

[0128] Therefore, the tobacco bud inhibitor of the present embodiment can effectively inhibit bud.

[0129] Example 2

[0130] The tobacco bud inhibitor in the embodiment includes the following components by mass percentage: indole-3-acetic acid methyl ester 5%, acetone solvent 40%, emulsifier 8%, n-pentanol 1%, glycerol 3%, and deionized water 43%. The emulsifier includes Farm Emulsifier No. 400.

[0131] The preparation method of the tobacco bud inhibitor in the embodiment is the same as that in Embodiment 1.

[0132] The tobacco bud inhibitor in the embodiment can effectively inhibit bud growth, reduce drug residues, and has qualified appearance stability, emulsion stability, and cold storage stability.

[0133] Embodiment 3

[0134] The tobacco bud inhibitor in the embodiment includes the following components by mass percentage: indole-3-acetic acid methyl ester 15%, acetone solvent 55%, emulsifier 8%, isopropyl alcohol 3%, propylene glycol 2%, and deionized water 17%. The emulsifier includes Farm Emulsifier No. 500.

[0135] The preparation method of the tobacco bud inhibitor in the embodiment is the same as that in Embodiment 1.

[0136] The tobacco bud inhibitor in the embodiment can effectively inhibit bud growth, reduce drug residues, and has qualified appearance stability, emulsion stability, and cold storage stability.

[0137] Embodiment 4

[0138] The tobacco bud inhibitor in the embodiment includes the following components by mass percentage: indole-3-acetic acid methyl ester 20%, acetone solvent 60%, emulsifier 12%, n-butanol 2%, polyethylene glycol 1%, and deionized water 5%. The emulsifier includes Farm Emulsifier No. 1602.

[0139] The preparation method of the tobacco bud inhibitor in the embodiment is the same as that in Embodiment 1.

[0140] The tobacco bud inhibitor in the embodiment can effectively inhibit bud growth, reduce drug residues, and has qualified appearance stability, emulsion stability, and cold storage stability.

[0141] Embodiment 5

[0142] The tobacco bud inhibitor in the embodiment includes the following components by mass percentage: indole-3-acetic acid methyl ester 10%, acetone solvent 50%, emulsifier 20%, n-butanol 2%, ethylene glycol 4%, and deionized water 14%. The emulsifier includes Farm Emulsifier No. 600.

[0143] The preparation method of the tobacco bud inhibitor in the embodiment is the same as that in Embodiment 1.

[0144] The tobacco bud inhibitor in the embodiment can effectively inhibit bud, reduce drug residues, and has qualified appearance stability, emulsion dispersion level of 2, qualified cold storage stability, qualified hot storage stability, and a PH value of 6.85.

[0145] Embodiment 6

[0146] The tobacco bud inhibitor in the embodiment includes the following components by mass percentage: indole-3-acetic acid methyl ester 10%, acetone solvent 50%, emulsifier 12%, n-butanol 3%, ethylene glycol 5%, and deionized water 20%. The emulsifier includes Farm Emulsion No. 500 and Farm Emulsion No. 1602, and the mass ratio of Farm Emulsion No. 1602 to Farm Emulsion No. 500 is 1:1.

[0147] The preparation method of the tobacco bud inhibitor in the embodiment is the same as that in Embodiment 1.

[0148] The tobacco bud inhibitor in the embodiment can effectively inhibit bud, reduce drug residues, and has qualified appearance stability, emulsion dispersion level of 2, qualified cold storage stability, qualified hot storage stability, and a PH value of 6.85.

[0149] Embodiment 7

[0150] The tobacco bud inhibitor in the embodiment includes the following components by mass percentage: indole-3-acetic acid methyl ester 10%, acetone solvent 50%, emulsifier 12%, n-butanol 5%, ethylene glycol 3%, and deionized water 20%. The emulsifier includes Farm Emulsion No. 500 and Farm Emulsion No. 1602, and the mass ratio of Farm Emulsion No. 1602 to Farm Emulsion No. 500 is 1:1.

[0151] The preparation method of the tobacco bud inhibitor in the embodiment is the same as that in Embodiment 1.

[0152] The tobacco bud inhibitor in the embodiment can effectively inhibit bud, reduce drug residues, and has qualified appearance stability, emulsion stability, emulsion dispersion level of 2, qualified cold storage stability, qualified hot storage stability, and a PH value of 6.95.

[0153] Embodiment 8

[0154] The tobacco bud inhibitor in the embodiment includes the following components by mass percentage: indole-3-acetic acid methyl ester 8%, acetone solvent 50%, emulsifier 15%, n-butanol 2%, ethylene glycol 5%, and deionized water 20%. The emulsifier includes Farm Emulsion No. 500 and Farm Emulsion No. 1602, and the mass ratio of Farm Emulsion No. 1602 to Farm Emulsion No. 500 is 1:1.

[0155] The preparation method of the tobacco bud inhibitor in the embodiment is the same as that in Embodiment 1.

[0156] The tobacco bud inhibitor in the embodiment can effectively inhibit bud, reduce drug residues, and has qualified appearance stability, emulsion stability, and cold storage stability.

[0157] Embodiment 9

[0158] The tobacco bud inhibitor in the embodiment includes the following components in percentage by mass: indole-3-acetic acid methyl ester 10%, acetone solvent 50%, emulsifier 15%, n-butanol 3%, ethylene glycol 3%, and deionized water 19%. The emulsifier includes Farm Emulsifier No. 500 and Farm Emulsifier No. 1602, and the mass ratio of Farm Emulsifier No. 1602 to Farm Emulsifier No. 500 is 1:1.

[0159] The preparation method of the tobacco bud inhibitor in the embodiment is the same as that in Embodiment 1.

[0160] The tobacco bud inhibitor in the embodiment can effectively inhibit bud, reduce drug residues, and has qualified cold storage stability.

[0161] In the following, the 200-fold solution of the tobacco bud inhibitor in Embodiments 1 to 9, the 200-fold solution of pendimethalin, the 200-fold solution of butralin, and the 200-fold solution of fluazuron are respectively applied to tobacco, and the half-life and 7-day residue on the tobacco are observed, and the results are shown in Table 5.

[0162] It should be noted that the half-life refers to the time required for the amount of a certain substance (such as a pesticide or a radioactive element) to decrease to half of the initial amount in the environment or in a living body. In the application, the half-life specifically refers to the time required for the 200-fold solution of the tobacco bud inhibitor in Embodiments 1 to 9, the 200-fold solution of pendimethalin, the 200-fold solution of butralin, and the 200-fold solution of fluazuron to decrease to half of the initial amount on the tobacco after being respectively applied to different tobaccos with the same initial amount. Multiple samples with the same initial amount of each bud inhibitor can be prepared and applied to different tobaccos to improve the accuracy of the results.

[0163] It should be noted that the 7-day residue refers to the residue of the bud inhibitor in the tobacco 7 days after the tobacco is applied with the bud inhibitor.

[0164] Table 5 Half-life and residue of different bud inhibitors on tobacco

[0165] Treatment Half-Life (days) 7-Day Residue (mg / kg) Example 1 1.63～4.32 0.04 Example 2 1.78～5.08 0.34 Example 3 1.70～4.99 0.22 Example 4 2.05～5.15 0.13 Example 5 1.97～4.83 0.31 Example 6 2.14～5.07 0.34 Example 7 1.58～4.89 0.28 Example 8 2.49～5.14 0.08 Example 9 1.69～4.44 0.05 Pendimethalin 2.56～5.97 0.40 Butralin 5.2～12.2 0.44 Flumetralin 3.3～5.2 1.53

[0166] As shown in Table 5, the 200-fold solution of the tobacco bud inhibitor in Example 1 was applied to tobacco, with a half-life of 1.63-4.32 days and a 7-day residual amount of 0.04 mg / kg. The 200-fold solution of the tobacco bud inhibitor in Example 2 was applied to tobacco, with a half-life of 1.78-5.08 days and a 7-day residual amount of 0.34 mg / kg. The 200-fold solution of the tobacco bud inhibitor in Example 3 was applied to tobacco, with a half-life of 1.70-4.99 days and a 7-day residual amount of 0.22 mg / kg. The 200-fold solution of the tobacco bud inhibitor in Example 4 was applied to tobacco, with a half-life of 2.05-5.15 days and a 7-day residual amount of 0.13 mg / kg. The 200-fold solution of the tobacco bud inhibitor in Example 5 was applied to tobacco, with a half-life of 1.97-4.83 days and a 7-day residual amount of 0.31 mg / kg. The 200-fold solution of the tobacco bud inhibitor in Example 6 was applied to tobacco, with a half-life of 2.14-5.07 days and a 7-day residual amount of 0.34 mg / kg. The 200-fold solution of the tobacco bud inhibitor in Example 7 was applied to tobacco, with a half-life of 1.58-4.89 days and a 7-day residual amount of 0.28 mg / kg. The 200-fold solution of the tobacco bud inhibitor in Example 8 was applied to tobacco, with a half-life of 2.49-5.14 days and a 7-day residual amount of 0.08 mg / kg. The 200-fold solution of the tobacco bud inhibitor in Example 9 was applied to tobacco, with a half-life of 1.69-4.44 days and a 7-day residual amount of 0.05 mg / kg.

[0167] As shown in Table 5, the 200-fold solution of butralin was applied to tobacco, with a half-life of 2.56-5.97 days and a 7-day residual amount of 0.40 mg / kg. The 200-fold solution of butamifos was applied to tobacco, with a half-life of 5.2-12.2 days and a 7-day residual amount of 0.44 mg / kg. The 200-fold solution of flumetralin was applied to tobacco, with a half-life of 3.3-5.2 days and a 7-day residual amount of 1.53 mg / kg.

[0168] It can be seen that the half-life and 7-day residual amount of the tobacco bud inhibitors in Examples 1-9 are all better than those of the bud inhibitors butralin, butamifos and flumetralin. Therefore, the tobacco bud inhibitors of the present application have the effects of fast degradation, easy degradation and low residual amount.

[0169] In the following, specific comparative examples are given to further illustrate the present disclosure.

[0170] Comparative Example 1

[0171] The bud inhibitor comprises the following components: 10% indole-3-acetic acid, 50% acetone solvent, 15% emulsifier, 4% n-butanol, 4% ethylene glycol and 17% deionized water. The emulsifier comprises Farm Emulsifier No. 500 and Farm Emulsifier No. 1602, and the mass ratio of Farm Emulsifier No. 1602 to Farm Emulsifier No. 500 is 1:1.

[0172] The difference between Comparative Example 1 and Example 1 is that the indole-3-acetic acid methyl ester in Example 1 is replaced by indole-3-acetic acid. The rest is the same as Example 1.

[0173] The 200 times solution of the sprout inhibitor in Comparative Example 1 and the 200 times solution of the tobacco sprout inhibitor in Example 1 are respectively applied to the axillary buds of tobacco, and the sprout inhibition rate and the sprout inhibition effect are shown in Table 6, and the half-life and the 7-day residual amount are shown in Table 7.

[0174] Comparative Example 2

[0175] The sprout inhibitor comprises the following components: indole butyric acid 10%, acetone solvent 50%, emulsifier 15%, n-butanol 4%, ethylene glycol 4%, and deionized water 17%. The emulsifier comprises Farm Emulsifier No. 500 and Farm Emulsifier No. 1602, and the mass ratio of Farm Emulsifier No. 1602 to Farm Emulsifier No. 500 is 1:1.

[0176] The difference between Comparative Example 2 and Example 1 is that the indole-3-acetic acid methyl ester in Example 1 is replaced by indole butyric acid. The rest is the same as Example 1.

[0177] The 200 times solution of the sprout inhibitor in Comparative Example 2 and the 200 times solution of the tobacco sprout inhibitor in Example 1 are respectively applied to the axillary buds of tobacco, and the sprout inhibition rate and the sprout inhibition effect are shown in Table 6, and the half-life and the 7-day residual amount are shown in Table 7.

[0178] Table 6 Sprout inhibition rate and sprout inhibition effect

[0179]

[0180] As shown in Table 6, T2 represents the 200 times solution of the tobacco sprout inhibitor in Example 1. T4 represents the 200 times solution of the sprout inhibitor in Comparative Example 1. T5 represents the 200 times solution of the sprout inhibitor in Comparative Example 2. The sprout inhibition rate and the sprout inhibition effect are as follows:

[0181] 14 days after application, the sprout inhibition rate of T2 is 100.00%. The sprout inhibition rate of T4 is 95.18%. The sprout inhibition rate of T5 is 85.54%. It can be seen that the sprout inhibition rate of T2 is higher than that of T4 and T5. In the same column of Table 6, the data marked a is significantly different from the data marked b and the data marked c.

[0182] 28 days after application, the sprout inhibition rate of T2 is 98.60%. The sprout inhibition rate of T4 is 47.39%. The sprout inhibition rate of T5 is 47.76%. It can be seen that the sprout inhibition rate of T2 is higher than that of T4 and T5. In the same column of Table 6, the data marked a is significantly different from the data marked b, the data marked a is significantly different from the data marked bc, and the data marked b is not significantly different from the data marked bc.

[0183] The bud inhibition rate of T2 was 96.53% at 42 days after application. The bud inhibition rate of T4 was 43.57%. The bud inhibition rate of T5 was 35.88%. It can be seen that the bud inhibition rate of T2 is higher than that of T4 and T5. Among them, in the same column of Table 6, the data marked a is significantly different from the data marked b, the data marked a is significantly different from the data marked bc, and the data marked b is not significantly different from the data marked bc.

[0184] The bud inhibition effect of T2 was 96.87% at 42 days after application. The bud inhibition effect of T4 was 21.16%. The bud inhibition effect of T5 was 20.00%. It can be seen that the bud inhibition effect of T2 is higher than that of T4 and T5. Among them, in the same column of Table 6, the data marked a is significantly different from the data marked b, the data marked a is significantly different from the data marked bc, and the data marked b is not significantly different from the data marked bc.

[0185] Therefore, the tobacco bud inhibitor of Example 1 can achieve effective bud inhibition.

[0186] Table 7 Half-life and 7-day residual amount

[0187] Treatment Half-Life (days) 7-Day Residue (mg / kg) Example 1 1.63～4.32 0.04 Comparative Example 1 2.85～5.67 0.88 Comparative Example 2 2.97～6.05 1.21

[0188] As shown in Table 7, the half-life of the 200-fold solution of the tobacco bud inhibitor in Example 1 applied to tobacco was 1.63-4.32 days, and the 7-day residual amount was 0.04 mg / kg. The half-life of the 200-fold solution of the bud inhibitor in Comparative Example 1 applied to tobacco was 2.85-5.67 days, and the 7-day residual amount was 0.88 mg / kg. The half-life of the 200-fold solution of the bud inhibitor in Comparative Example 2 applied to tobacco was 2.97-6.05 days, and the 7-day residual amount was 1.21 mg / kg.

[0189] It can be seen that the half-life and 7-day residual amount of the tobacco bud inhibitor of Example 1 are better than those of Comparative Examples 1 and 2. Therefore, the tobacco bud inhibitor of Example 1 has the effect of fast degradation, easy degradation, and low residual amount.

[0190] The above is only a preferred embodiment of the present application, and is not intended to limit the present application. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application. The above is only a preferred embodiment of the present application, and it should be pointed out that, for ordinary skilled in the art, without departing from the technical principles of the present application, a number of improvements and variations can be made, which should be regarded as the protection scope of the present application.

Claims

1. A tobacco bud suppressant, characterized by, The tobacco bud inhibitor comprises the following components in percentage by mass: indole-3-acetic acid methyl ester 10%, acetone solvent 50%, emulsifier 15%, n-butanol 4%, ethylene glycol 4%, and deionized water 17%; The emulsifier is agricultural emulsifier No. 500 and agricultural emulsifier No. 1602, and the mass ratio of agricultural emulsifier No. 1602 to agricultural emulsifier No. 500 is 1:

1.

2. A method for preparing a tobacco bud suppressant for use in the tobacco bud suppressant of claim 1, wherein The preparation method comprises the following steps: dissolving indole-3-acetic acid methyl ester in acetone solvent to form a solution; adding an emulsifier and n-butanol into the solution, stirring uniformly to form an oil phase; adding deionized water and ethylene glycol into the oil phase, stirring according to preset stirring parameters to obtain a tobacco bud inhibitor.

3. The method of claim 2, wherein the tobacco bud control agent is prepared by the steps of: The preset stirring parameters comprise: a stirring speed: 10,000 r / min to 12,000 r / min; and / or a stirring time: 1 min to 3 min.

Images