Respiratory system infection viroid sample detection device

Abstract

The invention relates to the technical field of pathogen detection, and discloses a respiratory system infection viroid sample detection device, which comprises a detection cylinder, a detection card is arranged in the detection cylinder, a one-way air inlet blowing nozzle is arranged at the port of the detection cylinder, and blown air is guided to be stored in an air cavity in the detection cylinder; the detection card comprises a gas production area, the surface is horizontally unfolded opposite to the gas outlet end of the blowing nozzle, and a gap in contact with the gas cavity is reserved in the gas production area; the transparent window is arranged outside the detection cylinder, and a display area is arranged in the middle of the detection card. The handheld portable blowing detection tube is adopted, breath containing droplets is guided to be intensively blown to a detection card sample introduction area, the structure is simple, sampling and detection are efficiently integrated, the infection risk in the use process is greatly reduced, medical staff are not needed for detection, a large amount of manpower and material resources are saved, and the detection efficiency is improved. The method is especially suitable for rapid screening of people closely contacted with a large number of suspected cases and definite cases, and the pressure of detection and diagnosis work can be effectively relieved.

Description

Technical Field

[0001] The present invention relates to the technical field of pathogen testing, and in particular to a respiratory infection virus sample testing device. Background Art

[0002] As the population size and density increase, the risk of large-scale outbreaks of epidemic infectious diseases is also increasing. Airborne transmission is the main route of transmission of epidemic respiratory diseases. Timely, rapid, accurate, simple, low-cost and high-throughput diagnosis of infected persons is the key to controlling the outbreak of the disease.

[0003] Chinese patent application No. 202010377997.8 discloses an integrated device for collecting and testing respiratory virus samples. By configuring a test card on the wearing device and using protective equipment to collect droplets as samples for direct testing, protection is integrated with sample collection and testing, and the virus in the droplet sample can be self-tested; no manual throat swab sampling and testing is required. However, there are many defects in wearing a mask. It needs to be worn, aligned with the mouth shape, and loosened. The fabric layer is easily contaminated during the process. It is difficult to properly preserve and handle the whole. There are many structures related to the internal detection structure, which is not suitable for flexible detection work over a large area.

[0004] To this end, the authors proposed a respiratory infection virus sample detection device to solve the above problems. Summary of the invention

[0005] The purpose of the present invention is to provide a respiratory infection virus sample detection device, which adopts a handheld and convenient blow-test tube, has a simple structure, and efficiently integrates sampling and detection, greatly reducing the risk of infection during use, so as to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solution: a respiratory infection virus sample detection device, comprising: The detection tube has a detection card installed inside, and a one-way air intake nozzle is provided at the port of the detection tube to guide the blown air to be stored in the air cavity inside the detection tube; The detection card includes an air collection area, the surface of which is horizontally extended toward the air outlet end of the blowing nozzle, and a gap for contacting the air cavity is left in the air collection area; The transparent window is arranged outside the detection tube and the display area is arranged in the middle of the detection card.

[0007] As an optional solution of the respiratory infection virus sample detection device described in the present invention, the detection cylinder includes a cylinder seat, which is a straight cylindrical structure, connected to a mouthpiece at one end of the cylinder seat, and a cylinder cover is detachably connected to one end of the cylinder seat where the mouthpiece is located; a protective cover is integrated inside the cylinder cover, which is an annular structure and seals and covers the mouthpiece.

[0008] As an optional solution of the respiratory infection virus sample detection device of the present invention, the mouthpiece is configured as a soft rubber tube, and a latex valve for one-way air intake from the outside is fixedly connected inside.

[0009] As an optional solution of the respiratory infection virus sample detection device described in the present invention, the detection card includes a PVC backing plate, and the upper surface is sequentially bonded with a sample injection area, a binding area, a display area and a water absorption area, and the edge of the PVC backing plate is fixedly connected to the inner wall of the top of the cylinder seat, and the space below forms an air cavity.

[0010] As an optional solution of the respiratory infection virus sample detection device of the present invention, one end of the sample inlet area is bent and tilted downward to form a gas sampling area to receive droplets exhaled from the mouth.

[0011] As an optional solution of the respiratory infection virus sample detection device of the present invention, strip-shaped air holes are formed on the air sampling area, and the air holes are axially aligned with the mouthpiece and extend inwardly to connect with the air cavity.

[0012] As an optional solution of the respiratory infection virus sample detection device of the present invention, the bottom of the gas sampling area is bent inward to form an extension area, and a ventilation gap is left on the inner wall of the bottom partition air cavity.

[0013] As an optional solution of the respiratory infection virus sample detection device described in the present invention, wherein: the surface of the binding area is distributed with a capture antibody of the virus S protein labeled with a color developer, the display area includes a detection line and a quality inspection line, the detection line is provided with a detection antibody of the S protein, and the quality inspection line is provided with a secondary antibody for binding to the capture antibody.

[0014] As an optional solution of the respiratory infection virus sample detection device described in the present invention, a movable telescopic cover is provided at the bottom of the cartridge seat, the edge of the telescopic cover is connected to the bottom of the cartridge seat through a folded airbag belt, a shaping belt is fixedly connected to the inner wall of the airbag belt, and the outer surface of the shaping belt is coated with an anhydrous copper sulfate coating for moisture discoloration detection.

[0015] As an optional solution of the respiratory infection virus sample detection device of the present invention, the inner wall of the telescopic cover is connected to the bottom wall of the cylinder seat through a limiting belt.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The respiratory infection virus sample detection device adopts a handheld and convenient blow-test tube. Air is blown safely through a one-way air intake nozzle to guide the breath containing droplets to be concentrated on the sampling area of ​​the detection card. Excess breath fills the air cavity in the tube and can still contaminate the sampling area, ensuring that the oral sample is collected in the sampling area for effective detection. The telescopic cover at the bottom of the tube detects whether the air volume is qualified, efficiently integrating sampling and detection, without the need for medical personnel to detect, saving a lot of manpower and material resources. The main structure is composed of a tube, a mouthpiece and a detection card. The structure is simple and efficient, and the tube is easy to handle in the later stage, which greatly reduces the risk of infection during use. It is particularly suitable for rapid screening of a large number of suspected cases and close contacts of confirmed cases, and can effectively alleviate the pressure of detection and diagnosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of a partial cross-sectional structure of the present invention; Figure 2 It is a schematic diagram of the expanded structure of the present invention; Figure 3 It is a schematic diagram of the partial structure of the detection card of the present invention; Figure 4 It is a schematic diagram of the cross-sectional structure of the cylinder cover of the present invention; Figure 5 It is a partial structural schematic diagram of the connection relationship of the telescopic cover of the present invention.

[0018] In the figure: 1. detection cylinder; 2. cylinder seat; 3. cylinder cover; 4. detection card; 5. transparent window; 6. blowing nozzle; 7. latex valve; 8. air cavity; 9. PVC backing plate; 10. injection area; 11. combination area; 12. display area; 13. water absorption area; 14. gas collection area; 15. extension area; 16. air hole; 17. protective cover; 18. telescopic cover; 19. air bag belt; 20. shaping belt; 21. limiting belt. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] See also Figure 1-Figure 5 The present invention provides a technical solution: a respiratory infection virus sample detection device, comprising: The detection tube 1 includes a tube base 2, which is a straight cylindrical structure and can be made of transparent plastic material. One end of the tube base 2 is connected to the blowing nozzle 6, and the end of the tube base 2 where the blowing nozzle 6 is located can be detachably connected to the tube cover 3. The detachable connection method is threaded connection or sleeve connection, both of which are conventional cover sealing methods, and the specific structure will not be described in detail.

[0021] A test card 4 is installed inside the test tube 1. The test card 4 includes a PVC backing plate 9, and a sample injection area 10, a binding area 11, a display area 12 and a water absorption area 13 are sequentially bonded to the upper surface. The edge of the PVC backing plate 9 is fixedly connected to the inner wall of the top of the tube base 2, and an air cavity 8 is formed in the space below the test card 4.

[0022] A one-way air intake nozzle 6 is provided at the end of the detection tube 1 to guide the blown air to be stored in an air cavity 8 in the detection tube 1 .

[0023] In order to facilitate the mouthpiece 6 to guide the oral droplets to the test card 4: One end of the sample inlet area 10 is bent and tilted downward to form a gas collection area 14. The surface of the gas collection area 14 is horizontally extended toward the gas outlet end of the mouthpiece 6 to receive droplets exhaled from the oral cavity.

[0024] It should be noted that the sample inlet area 10 and the water absorption area 13 are filter paper sheets made of cellulose material, and the water absorption area 13 is used to absorb excess liquid. The binding area 11 overlapped at one end of the sample inlet area 10 is a glass fiber filter membrane, which is also a colloidal gold binding pad, and the capture antibody of the virus S protein marked with colloidal gold particles (red) is distributed on the surface. The display area 12 overlapped between the binding area 11 and the water absorption area 13 is a nitrocellulose membrane, which is convenient for the flow of droplet liquid.

[0025] The display area 12 includes a detection line and a quality inspection line. The detection line is provided with a detection antibody for the S protein, and the quality inspection line is provided with a secondary antibody for binding to the capture antibody.

[0026] When in use, when both the test line and the quality inspection line are red, it indicates a positive result. If the test line does not turn red but the quality inspection line turns red, the result is negative; if the test line turns red but the quality inspection line does not turn red, the result is invalid.

[0027] In this embodiment, the virus S protein is selected as the antigen, the S protein sequence is connected to the PET 28a vector, and it is transformed into E. coli BL21 for expression. After purification, the product protein can be used as an antigen for detection.

[0028] In some embodiments, the device can also be used to detect coronaviruses, such as SARS virus, new coronavirus, MERS virus, etc. At this time, if the viral S protein cannot be directly obtained, the capture antibody of the viral S protein can also be prepared by the following method: The gene of the part of the human ACE2 protein that binds to the viral S protein (or RBD) is tagged with GST and transformed into E. coli BL21 for expression.

[0029] The process of coronavirus invading host cells mainly depends on the spike protein (S protein) on the surface of the virus, and the receptor of coronavirus on human cells is angiotensin-converting enzyme II (ACE2). Therefore, for coronavirus, the part of ACE2 protein that binds to S protein is expressed with GST tag, which can also be used as a detection antibody. For some coronaviruses that are temporarily unable to obtain antibodies, this method can also be used as an alternative, which can achieve the same detection effect as directly using antibodies.

[0030] As a further improvement of this embodiment, a transparent window 5 is provided on the outside of the detection tube 1, and the transparent window 5 detects the display area 12 in the middle of the detection card 4 for the outside to view the detection results.

[0031] To prevent the breath blown into the test tube 1 from escaping: In this embodiment, the mouthpiece 6 is configured as a soft rubber tube, and a latex valve 7 for one-way air intake from the outside is fixedly connected inside. Air can only be blown in one direction, and the mouthpiece 6 is prevented from leaking the gas in the tube, thereby preventing external leakage and pollution.

[0032] In order to further improve the sealing safety of the blowing nozzle 6, a protective cover 17 is integrally connected to the interior of the tube cover 3, which has an annular structure and seals and covers the blowing nozzle 6 to improve safety.

[0033] In order to increase the droplets' adhesion to the injection area 10 during blowing: In this embodiment, strip-shaped air holes 16 are formed on the air collection area 14, and the air holes 16 are axially aligned with the blowing nozzle 6 and extend inwardly to connect with the air cavity 8; the bottom of the air collection area 14 is bent inwardly to form an extension area 15, and a ventilation gap is left at the bottom of the air collection area 14 between the inner walls of the air cavity 8.

[0034] When in use, air is taken in by the mouthpiece 6 and first passes through the air hole 16. The annular inner wall surface of the air hole 16 increases the contact area, allowing the inflowing breath droplets to be intercepted by the annular inner wall surface, and the air hole 16 does not hinder the blowing, allowing the breath to enter the air cavity 8 and be maintained for collection.

[0035] In addition, the bottom of the gas sampling area 14 is bent inward to form an extension area 15. The extension area 15 expands the contact surface to contact the breath stored in the air cavity 8, ensuring that the sampling area collects the oral sample for effective detection.

[0036] That is, a handheld and convenient air blowing test tube is used, and air is blown safely through a one-way air intake nozzle to guide the breath containing droplets to be concentrated on the sampling area 10 of the test card 4. The excess breath fills the air cavity 8 in the tube and can still contaminate the sampling area 10, ensuring that the sampling area collects oral samples for effective testing.

[0037] The overall structure efficiently integrates sampling and testing, and does not require medical personnel for testing, saving a lot of manpower and material resources. The main structure is composed of a bobbin, a mouthpiece and a test card. The structure is simple and efficient, and the bobbin is easy to handle later, which greatly reduces the risk of infection during use. It is particularly suitable for rapid screening of a large number of suspected cases and close contacts of confirmed cases, and can effectively alleviate the pressure of testing and diagnosis.

[0038] As a further improvement of this embodiment, in order to prevent some people from perfunctorily treating the detection work: In this embodiment, a movable telescopic cover 18 is provided at the bottom of the cartridge seat 2, and the edge of the telescopic cover 18 is connected to the bottom of the cartridge seat 2 through a folded airbag belt 19. A shaping belt 20 is fixedly connected to the inner wall of the airbag belt 19, and the outer surface of the shaping belt 20 is coated with an anhydrous copper sulfate coating for moisture discoloration detection. When in use, the blowing volume needs to reach a certain level, and the folded airbag belt 19 needs to be gradually blown up until the airbag belt 19 is columnar. Among them, the shaping belt 20 is set as a plastic belt. When the airbag belt 19 is bulged and the shaping belt 20 is straightened, the shape of the limiting airbag belt 19 does not bulge outward. At this time, the airbag belt 19 has the same outer diameter as the cartridge seat 2, and the cylindrical structure is maintained.

[0039] When the airbag belt 19 is inflated, the inner surface of the shaping belt 20 is unfolded, and the anhydrous copper sulfate coating on the inner surface contacts the moist breath in the air cavity 8. The anhydrous copper sulfate coating changes from white to blue after absorbing water. The color indicates that the blowing is qualified, avoids missing screening, and improves the accuracy of the detection work.

[0040] Furthermore, the inner wall of the telescopic cover 18 is connected to the bottom wall of the cylinder seat 2 through a limiting belt 21, and the limiting belt 21 is set as a steel belt to strengthen the structure of the airbag belt 19 and the telescopic cover 18, limit and stabilize the position of the telescopic cover 18 and the airbag belt 19, avoid excessive blowing by the inspector to damage the inspection cylinder 1, and improve the stability of use.

[0041] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A respiratory infection virus sample detection device, characterized in that: include: A detection tube (1) is provided with a detection card (4) installed inside, and a one-way air intake nozzle (6) is provided at the port of the detection tube (1) to guide the blown air to be stored in an air cavity (8) inside the detection tube (1); The detection card (4) comprises an air collection area (14), the surface of which is horizontally extended toward the air outlet end of the blowing nozzle (6), and a gap is left in the air collection area (14) for contacting the air cavity (8); A transparent window (5) is arranged outside the detection tube (1), and a display area (12) is arranged in the middle of the detection card (4).

2. A respiratory infection virus sample detection device according to claim 1, characterized in that: The detection cylinder (1) comprises a cylinder seat (2) which is a straight cylindrical structure, one end of which is connected to a mouthpiece (6), and one end of which, where the mouthpiece (6) is located, is detachably connected to a cylinder cover (3); a protective cover (17) is integrally connected to the inside of the cylinder cover (3) and is annular in structure, sealingly wrapping around and covering the mouthpiece (6).

3. A respiratory infection virus sample detection device according to claim 1, characterized in that: The mouthpiece (6) is configured as a soft rubber tube, and a latex valve (7) for one-way air intake from the outside is fixedly connected inside.

4. A respiratory infection virus sample detection device according to claim 1, characterized in that: The detection card (4) comprises a PVC backing plate (9), the upper surface of which is adhered in sequence with a sample injection area (10), a binding area (11), a display area (12) and a water absorption area (13); the edge of the PVC backing plate (9) is fixedly connected to the top inner wall of the cartridge seat (2), and the space below forms an air cavity (8).

5. A respiratory infection virus sample detection device according to claim 4, characterized in that: One end of the sample inlet area (10) is bent and tilted downward to form a gas collection area (14) for receiving droplets exhaled from the oral cavity.

6. A respiratory infection virus sample detection device according to claim 5, characterized in that: The gas collection area (14) is formed with strip-shaped air holes (16), which are axially aligned with the blowing nozzle (6) and extend inwardly to communicate with the air cavity (8).

7. A respiratory infection virus sample detection device according to claim 6, characterized in that: The bottom of the gas extraction area (14) is bent inward to form an extension area (15), and a ventilation gap is left on the inner wall of the bottom spacer air cavity (8).

8. A respiratory infection virus sample detection device according to claim 7, characterized in that: The surface of the binding area (11) is distributed with a capture antibody for the virus S protein labeled with a color developer, and the display area (12) comprises a detection line and a quality inspection line, the detection line is provided with a detection antibody for the S protein, and the quality inspection line is provided with a secondary antibody for binding to the capture antibody.

9. A respiratory infection virus sample detection device according to claim 2, characterized in that: A movable telescopic cover (18) is provided at the bottom of the cartridge seat (2), the edge of the telescopic cover (18) is connected to the bottom of the cartridge seat (2) via a folded airbag belt (19), a shaping belt (20) is fixedly connected to the inner wall of the airbag belt (19), and the outer surface of the shaping belt (20) is coated with an anhydrous copper sulfate coating for moisture discoloration detection.

10. A respiratory infection virus sample detection device according to claim 9, characterized in that: The inner wall of the telescopic cover (18) is connected to the bottom wall of the cylinder seat (2) via a limiting belt (21).

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