Veterinary traditional Chinese medicine granule for preventing and treating atypical bursa of Fabricius of poultry and application thereof

Abstract

The invention relates to the technical field of traditional Chinese veterinary medicine preparations, and provides veterinary traditional Chinese medicine granules for preventing and treating atypical bursa of Fabricius of poultry and application of the veterinary traditional Chinese medicine granules. The veterinary traditional Chinese medicine granules for preventing and treating atypical bursa of Fabricius of poultry are prepared from the following raw materials in parts by weight: 80 to 120 parts of radix astragali seu hedysari, 20 to 60 parts of radix codonopsis, 15 to 30 parts of radix angelicae sinensis, 10 to 30 parts of liquorice root, 30 to 50 parts of radix salviae miltiorrhizae, 40 to 60 parts of herba artemisiae annuae, 30 to 50 parts of poria cocos, 20 to 40 parts of stir-fried rhizoma dioscoreae, 15 to 30 parts of radix puerariae, 15 to 30 parts of radix bupleuri and 1 to 10 parts of folium ginseng. According to the technical scheme, the problem that the production performance is reduced due to the atypical bursal disease of poultry in the related technology is solved.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese veterinary medicine preparations, and in particular to a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry and an application thereof. Background Art

[0002] Atypical bursal disease is an infectious disease of poultry caused by infectious bursal virus. Atypicality is mainly relative to typical bursal disease. Its symptoms, lesions and other manifestations are not as obvious and typical as typical cases. Therefore, the disease is not recognized and recognized by breeders, let alone investment in prevention and treatment. However, at present, based on the statistical analysis of laboratory tests and report data collected from diseased materials, the incidence detection rate of atypical bursal inflammation has been gradually increasing from 2021 to the present. Improper prevention and treatment will not only increase the cost of medication, but also delay the performance of poultry production, resulting in the suppression of poultry's immune function, thereby greatly reducing breeding income. Therefore, it is very necessary to develop a veterinary Chinese medicine granule for the prevention and treatment of atypical bursal disease in poultry. Summary of the invention

[0003] The present invention provides a veterinary Chinese medicine granule for preventing and treating atypical bursal disease of poultry and application thereof, which solves the problem of decreased production performance caused by atypical bursal disease of poultry in the related art.

[0004] The technical scheme of the present invention is as follows: The present invention proposes a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry, and the raw materials include the following components in parts by weight: 80-120 parts of astragalus, 20-60 parts of codonopsis, 15-30 parts of angelica, 10-30 parts of licorice, 30-50 parts of salvia miltiorrhiza, 40-60 parts of artemisia annua, 30-50 parts of poria, 20-40 parts of fried yam, 15-30 parts of kudzu root, 15-30 parts of bupleurum, and 1-10 parts of ginseng leaves.

[0005] As a further technical solution, the raw materials include the following components in parts by weight: 90-110 parts of astragalus, 30-50 parts of codonopsis, 20-28 parts of angelica, 15-25 parts of licorice, 35-45 parts of salvia, 45-55 parts of artemisia annua, 35-45 parts of poria, 25-35 parts of fried yam, 20-28 parts of kudzu root, 20-28 parts of bupleurum, and 3-8 parts of ginseng leaves.

[0006] The present invention also provides a method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry, comprising the following steps: S1, adding Astragalus to an extractant for extraction, filtering to obtain a residue and a filtrate, and concentrating, extracting, eluting, separating, concentrating twice, and filtering the filtrate to obtain extract I; S2, soaking the remaining raw materials in water, adding the medicinal residue, decocting with water to obtain a decoction, and concentrating and filtering the decoction under reduced pressure to obtain extract II; S3, the extract I, extract II and auxiliary materials are mixed evenly, granulated, dried and granulated to obtain a granule semi-finished product, and packaged to obtain a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry; The auxiliary materials include powdered sugar and water-melted realgar.

[0007] As a further technical solution, the extractant includes ethanol, and the volume mass ratio of the extractant to the astragalus is 10~12:1.

[0008] As a further technical solution, the extractant also includes 3-methylimidazolium bromide, and the volume ratio of the ethanol to the 3-methylimidazolium bromide is 15-20:1.

[0009] In the present invention, ethanol and 3-methylimidazolium bromide are added as extractants at the same time, and the synergistic effect of the two is exerted to further improve the dispersion of the effective ingredients of Astragalus, enhance the content of the effective ingredients in the atypical bursal veterinary Chinese medicine granules, further enhance the prevention and treatment effect of the veterinary Chinese medicine granules, and improve the production performance of poultry.

[0010] As a further technical solution, the 3-methylimidazolium bromide includes one or more of 1-aminopropyl-3-methylimidazolium bromide, 1-butyl-3-methylimidazolium bromide, and 1-decyl-3-methylimidazolium bromide.

[0011] As a further technical solution, the concentration of the ethanol is 70wt%.

[0012] As a further technical solution, the active ingredients of the extractant used in the extraction are hydroxyethyl methylcellulose and tetrabutylammonium tetrafluoroborate, and the mass ratio of the hydroxyethyl methylcellulose to the tetrabutylammonium tetrafluoroborate is 4-5:2.

[0013] As a further technical solution, the volume mass ratio of the extractant to the astragalus is 0.12~0.15:1.

[0014] As a further technical solution, the extractant consists of an effective ingredient and a solvent.

[0015] As a further technical solution, the concentration of the active ingredient in the extractant is 30wt%~40wt%, and the solution used in the extractant is water.

[0016] As a further technical solution, the mass ratio of extract I, extract II, powdered sugar and water-frozen realgar is 0.12:0.5:4.37:0.01.

[0017] As a further technical solution, the extraction time in S1 is 5-7 hours, and the extraction temperature is 60-65°C.

[0018] As a further technical solution, the soaking time in S2 is 3-5 hours, and the decocting time is 1-2 hours.

[0019] As a further technical solution, the mass ratio of the remaining raw materials in S2 to water is 1:8.

[0020] As a further technical solution, the sieve specifications used for filtration in S1 and S2 are both 300 mesh.

[0021] The present invention also proposes the use of the veterinary Chinese medicine granules for preventing and treating atypical bursa of Fabricius in poultry or the veterinary Chinese medicine granules for preventing and treating atypical bursa of Fabricius in poultry prepared by the preparation method in preventing and treating atypical bursa of Fabricius in poultry.

[0022] The working principle and beneficial effects of the present invention are: In the present invention, ethanol and 3-methylimidazolium bromide are used as extractants at the same time to extract the effective components of Astragalus. The synergistic effect of the two is exerted to promote the dissociation and dissolution of the effective components in Astragalus, thereby improving the prevention and treatment effect of the atypical bursal veterinary Chinese medicine granules and improving the production performance of poultry. DETAILED DESCRIPTION

[0023] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] In the following examples and comparative examples: Hydroxyethyl methyl cellulose: model is HEMC, manufacturer is Jinan Zhongao Chemical Co., Ltd.

[0025] Example 1 A method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry comprises the following steps: S1. Add 90 parts of Astragalus to 900 parts of extractant, extract at 60° C. for 7 hours, filter to obtain drug residues and filtrate, concentrate the filtrate, add 10.8 parts of extractant to extract, remove impurities and concentrate under reduced pressure, filter with a 300-mesh oscillating sieve to remove char residue, and obtain extract I, wherein the extractant is 70wt% ethanol, the concentration of the active ingredient in the extractant is 30wt%, the active ingredient in the extractant is composed of hydroxyethyl methylcellulose and tetrabutylammonium tetrafluoroborate in a mass ratio of 4:2, and the solvent of the extractant is water; S2. Add 1984 parts of water to 30 parts of Codonopsis pilosula, 20 parts of Angelica sinensis, 15 parts of Licorice, 35 parts of Salvia miltiorrhiza, 45 parts of Artemisia annua, 35 parts of Poria, 25 parts of stir-fried Chinese yam, 20 parts of Pueraria root, 20 parts of Bupleurum chinense, and 3 parts of ginseng leaves, and soak for 3 hours. Add the residues and decoct for 1 hour to obtain a decoction. After the decoction is concentrated under reduced pressure, filter it with a 300-mesh oscillating sieve to remove the charred residue to obtain extract II. S3. Put extract I, extract II, powdered sugar and water-frozen realgar into a wet mixer at a mass ratio of 0.12:0.5:4.37:0.01, mix evenly to prepare an ointment, and obtain a granular semi-finished product through granulation, drying and granulation. The semi-finished product can be packaged to obtain the veterinary Chinese medicine granules for preventing and treating atypical bursa of Fabricius in poultry.

[0026] Example 2 A method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry comprises the following steps: S1. Add 100 parts of Astragalus to 1100 parts of extractant, extract at 62° C. for 6 hours, filter to obtain drug residue and filtrate, concentrate the filtrate, add 13 parts of extractant to extract, remove impurities and concentrate under reduced pressure, filter with a 300-mesh oscillating sieve to remove char residue, and obtain extract I, wherein the extractant is 70wt% ethanol, the concentration of the active ingredient in the extractant is 35wt%, the active ingredient in the extractant is composed of hydroxyethyl methylcellulose and tetrabutylammonium tetrafluoroborate in a mass ratio of 4:2, and the solvent of the extractant is water; S2. Add 2376 parts of water to 40 parts of Codonopsis pilosula, 24 parts of Angelica sinensis, 20 parts of Licorice, 40 parts of Salvia miltiorrhiza, 48 parts of Artemisia annua, 40 parts of Poria, 28 parts of stir-fried Chinese yam, 24 parts of Pueraria root, 24 parts of Bupleurum, and 5 parts of Ginseng leaf, and soak for 4 hours. Add the residues and decoct for 1.5 hours to obtain a decoction. After the decoction is concentrated under reduced pressure, it is filtered through a 300-mesh oscillating sieve to remove the charred residue to obtain extract II. S3. Put extract I, extract II, powdered sugar and water-frozen realgar into a wet mixer at a mass ratio of 0.12:0.5:4.37:0.01, mix evenly to prepare an ointment, and obtain a granular semi-finished product through granulation, drying and granulation. The semi-finished product can be packaged to obtain the veterinary Chinese medicine granules for preventing and treating atypical bursa of Fabricius in poultry.

[0027] Example 3 A method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry comprises the following steps: S1. Add 110 parts of Astragalus to 1280 parts of extractant, extract at 65°C for 5h, filter to obtain drug residue and filtrate, concentrate the filtrate, add 16.5 parts of extractant to extract, remove impurities and concentrate under reduced pressure, filter with a 300-mesh oscillating sieve to remove char residue, and obtain extract I, wherein the extractant is 70wt% ethanol, the concentration of the active ingredient in the extractant is 40wt%, the active ingredient in the extractant is composed of hydroxyethyl methylcellulose and tetrabutylammonium tetrafluoroborate in a mass ratio of 4:2, and the solvent of the extractant is water; S2. Add 2784 parts of water to 50 parts of Codonopsis pilosula, 28 parts of Angelica sinensis, 25 parts of Licorice, 45 parts of Salvia miltiorrhiza, 55 parts of Artemisia annua, 45 parts of Poria, 35 parts of stir-fried Chinese yam, 28 parts of Pueraria root, 28 parts of Bupleurum, and 8 parts of Ginseng leaf, and soak for 5 hours. Add the residues and decoct for 2 hours to obtain a decoction. After the decoction is concentrated under reduced pressure, filter it with a 300-mesh oscillating sieve to remove the charred residue to obtain extract II. S3. Put extract I, extract II, powdered sugar and water-frozen realgar into a wet mixer at a mass ratio of 0.12:0.5:4.37:0.01, mix evenly to prepare an ointment, and obtain a granular semi-finished product through granulation, drying and granulation. The semi-finished product can be packaged to obtain the veterinary Chinese medicine granules for preventing and treating atypical bursa of Fabricius in poultry.

[0028] Example 4 Compared with Example 3, the difference of Example 4 is that ethanol is replaced by an equal volume of 1-aminopropyl-3-methylimidazolium bromide.

[0029] Example 5 Compared with Example 4, the difference of Example 5 is that ethanol is replaced by ethanol and 1-aminopropyl-3-methylimidazolium bromide in a volume ratio of 15:1.

[0030] Example 6 Compared with Example 4, the difference of Example 6 is that ethanol is replaced by ethanol and 1-butyl-3-methylimidazolium bromide in a volume ratio of 15:1.

[0031] Example 7 Compared with Example 4, the difference of Example 7 is that ethanol is replaced by ethanol and 1-decyl-3-methylimidazolium bromide in a volume ratio of 15:1.

[0032] Example 8 Compared with Example 5, the difference in Example 8 is that the volume ratio of ethanol to 1-aminopropyl-3-methylimidazolium bromide is 15:2.

[0033] Example 9 Compared with Example 8, the difference in Example 9 is that the mass ratio of hydroxyethyl methylcellulose to tetrabutylammonium tetrafluoroborate is 5:2.

[0034] Comparative Example 1 Compared with Example 3, the difference in Comparative Example 1 is that ethanol is replaced by an equal volume of water.

[0035] Comparative Example 2 Compared with Example 9, the difference in Comparative Example 2 is that tetrabutylammonium tetrafluoroborate is replaced by an equal mass of ammonium dihydrogen phosphate.

[0036] Test 1 After freeze-drying the extract I prepared in Examples 1 to 9 and Comparative Examples 1 to 2, the content of astragaloside I was determined by high performance liquid chromatography. The detection method refers to the 2020 edition of the Chinese Pharmacopoeia "Pharmacopoeia of the People's Republic of China".

[0037] The test results are shown in Table 1 below: Table 1 Astragaloside content in Examples 1 to 9 and Comparative Examples 1 to 2

[0038] Compared with Comparative Example 1, Example 3 uses ethanol as the extractant. As a result, the content of astragaloside IV in Example 3 is higher than that in Comparative Example 1, indicating that when ethanol is used as the extractant, the content of astragaloside IV in the finished granules can be increased.

[0039] Compared with Examples 3 and 4, Examples 5 to 8 simultaneously added ethanol and 3-methylimidazolium bromide as extractants, and the results showed that the content of astragaloside IV in Examples 5 to 8 was higher than that in Examples 3 and 4, indicating that ethanol and 3-methylimidazolium bromide played a synergistic role and could increase the content of astragaloside IV in the Chinese medicine granules. In Examples 5 to 7, different types of 3-methylimidazolium bromide were added, and the content of astragaloside IV in the Chinese medicine granules for veterinary use prepared in Example 5 was higher than that in Examples 6 and 7, indicating that when the added 3-methylimidazolium bromide was 1-aminopropyl-3-methylimidazolium bromide, the content of astragaloside IV in the granules could be further increased.

[0040] Compared with Comparative Example 2, Example 9 uses hydroxyethyl methyl cellulose and tetrabutylammonium tetrafluoroborate as extractants. As a result, the content of astragaloside IV in Example 9 is higher than that in Comparative Example 2, indicating that when hydroxyethyl methyl cellulose and tetrabutylammonium tetrafluoroborate are used as extractants, the content of astragaloside IV in the obtained veterinary Chinese medicine granules is higher.

[0041] Test 2: In a broiler farm in Luquan, Shijiazhuang, some broilers in one chicken house showed a slight state of mental depression. Dissection showed that the bursa of Fabricius was atrophic, the bursa of Fabricius was pale and had mucus exudation, and some bursa of Fabricius had bleeding or cheesy matter in the cavity. It was suspected that the bursa of Fabricius was diseased with atypical bursa of Fabricius. 240 14-day-old broilers with similar weight in the chicken house were randomly selected as experimental subjects, isolated and fed, and randomly divided into 12 groups, 20 in each group, namely 1 control group and 11 test groups. The test groups were fed with the veterinary Chinese medicine granules prepared in Examples 1 to 9 and Comparative Examples 1 to 2, once a day, 0.25g per chicken, and 450kg of concentrated drinking water was added for every 1kg of water. After continuous feeding of Chinese medicine granules for 5 days and normal feeding for 16 days, the weight changes and feed consumption of each group of experimental broilers were recorded, and the feed-to-weight ratio was calculated, and the feed-to-weight ratio = average feed consumption / average weight gain. The test results are shown in Table 2 below: Table 2 Clinical trial results of Chinese veterinary medicine granules prepared in the control group, Examples 1 to 9 and Comparative Examples 1 to 2

[0042] Compared with the control group, the experimental group was fed with veterinary Chinese herbal granules daily. The results showed that the average feed consumption and feed-to-weight ratio of the experimental group were lower than those of the control group, indicating that veterinary Chinese herbal granules have the effect of preventing and treating atypical bursa of Fabricius in poultry and improving the production performance of poultry. After 21 days of observation, the weight of broilers in the experimental group caught up with that of healthy broilers in the same batch.

[0043] Compared with Comparative Example 1, Example 3 uses ethanol as the extractant. As a result, the average feed consumption and feed-to-weight ratio of the broilers fed with Example 3 are both lower than those of Comparative Example 1, indicating that when ethanol is used as the extractant, the content of active ingredients in the veterinary Chinese medicine granules obtained in Example 3 is higher, and the effect of improving the production performance of poultry is better.

[0044] Compared with Examples 3 and 4, Examples 5 to 8 simultaneously added ethanol and 3-methylimidazolium bromide as extractants. As a result, the average feed consumption and feed-to-weight ratio of the broilers fed with Examples 5 to 8 were less than those of Comparative Example 1, indicating that ethanol and 3-methylimidazolium bromide exerted a synergistic effect, which could further increase the content of the active ingredient in the veterinary Chinese medicine granules and further enhance the production performance of poultry.

[0045] In Examples 5 to 7, different types of 3-methylimidazolium bromide were added as extractants. As a result, the average feed consumption and feed-to-weight ratio of the broilers fed with Example 5 were less than those of Examples 6 and 7, indicating that when the added 3-methylimidazolium bromide is 1-aminopropyl-3-methylimidazolium bromide, the content of the active ingredient in the veterinary Chinese medicine granules can be further increased, and the production performance of poultry can be further enhanced.

[0046] Compared with Comparative Example 2, Example 9 uses hydroxyethyl methylcellulose and tetrabutylammonium tetrafluoroborate as extractants. As a result, the average feed consumption and feed-to-weight ratio of broilers fed with Example 9 are less than those of Comparative Example 2, indicating that when hydroxyethyl methylcellulose and tetrabutylammonium tetrafluoroborate are used as extractants, the content of active ingredients in the obtained veterinary Chinese medicine granules is higher and the production performance of poultry is better.

[0047] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry, characterized in that: The raw materials include the following components in parts by weight: 80-120 parts of astragalus, 20-60 parts of codonopsis, 15-30 parts of angelica, 10-30 parts of licorice, 30-50 parts of salvia, 40-60 parts of artemisia annua, 30-50 parts of poria, 20-40 parts of fried yam, 15-30 parts of kudzu root, 15-30 parts of bupleurum, and 1-10 parts of ginseng leaves.

2. The veterinary Chinese medicine granules for preventing and treating atypical bursa of Fabricius in poultry according to claim 1, characterized in that: The raw materials include the following components in parts by weight: 90-110 parts of astragalus, 30-50 parts of codonopsis, 20-28 parts of angelica, 15-25 parts of liquorice, 35-45 parts of salvia, 45-55 parts of artemisia annua, 35-45 parts of poria, 25-35 parts of stir-fried yam, 20-28 parts of kudzu root, 20-28 parts of bupleurum, and 3-8 parts of ginseng leaves.

3. A method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry according to claim 1 or 2, characterized in that: The following steps are involved: S1, adding Astragalus to an extractant for extraction, filtering to obtain a residue and a filtrate, and concentrating, extracting, re-concentrating, and filtering the filtrate to obtain extract I; S2, soaking the remaining raw materials in water, adding the medicinal residue, decocting with water to obtain a decoction, and concentrating and filtering the decoction under reduced pressure to obtain extract II; S3, the extract I, extract II and auxiliary materials are mixed evenly, granulated, dried and granulated to obtain a granule semi-finished product, and packaged to obtain a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry; The auxiliary materials include powdered sugar and water-melted realgar; The extractant includes ethanol.

4. The method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry according to claim 3, characterized in that: The volume mass ratio of the extractant to the astragalus is 10-12 mL: 1 g.

5. The method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry according to claim 3, characterized in that: The extractant also includes 3-methylimidazolium bromide, and the volume ratio of the ethanol to the 3-methylimidazolium bromide is 15-20:

1.

6. The method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry according to claim 4, characterized in that: The 3-methylimidazolium bromide includes one or more of 1-aminopropyl-3-methylimidazolium bromide, 1-butyl-3-methylimidazolium bromide, and 1-decyl-3-methylimidazolium bromide.

7. The method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry according to claim 3, characterized in that: The active ingredients of the extractant used in the extraction are hydroxyethyl methyl cellulose and tetrabutylammonium tetrafluoroborate, and the mass ratio of the hydroxyethyl methyl cellulose to the tetrabutylammonium tetrafluoroborate is 4-5:

2.

8. The method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry according to claim 7, characterized in that: The volume mass ratio of the extractant to the astragalus is 0.12-0.15:

1.

9. The method for preparing a veterinary Chinese medicine granule for preventing and treating atypical bursa of Fabricius in poultry according to claim 3, characterized in that: The mass ratio of the extract I, extract II, powdered sugar and water-melted realgar is 0.12:0.5:4.37:0.

01.

10. Use of the veterinary Chinese medicinal granules for preventing and treating atypical bursa of Fabricius in poultry according to any one of claims 1 to 2 or the veterinary Chinese medicinal granules for preventing and treating atypical bursa of Fabricius in poultry obtained by the preparation method according to any one of claims 3 to 9 in the prevention and treatment of atypical bursa of Fabricius in poultry.

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