Oral ulcer film containing various plant extracts and preparation method thereof

Abstract

The invention provides an oral ulcer film containing various plant extracts. The oral ulcer film is prepared by drying the following raw materials in percentage by mass: 0.1%-1.5% of a licochalcone extract, 0.25%-0.5% of a reed rhizome extract, 0.25%-0.5% of a cistanche deserticola extract, 0.25%-0.5% of a rose extract, 2.58% of polyvinyl alcohol, 0.28% of sodium carboxymethyl cellulose, 0.46% of glycerol and the balance of water. The invention also provides a preparation method. The oral ulcer membrane disclosed by the invention can improve the oral inflammation problem and has good effects of easing pain and diminishing swelling.

Description

Technical Field

[0001] The invention belongs to the technical field of oral ulcer membranes, and particularly relates to an oral ulcer membrane containing multiple plant extracts and a preparation method thereof. Background Art

[0002] The pathogenesis of oral ulcers has not been fully elucidated so far, and the histopathological manifestations are non-specific inflammation. Most scholars believe that the occurrence of oral ulcers is the result of the combined effects of multiple factors. Immunity, genetics and environment may be the "triple factors" of oral ulcers, that is, genetic background and appropriate environmental factors (including psychoneurological constitution, psychological and behavioral state, life, work and social environment, etc.) can trigger abnormal immune responses and cause characteristic lesions of oral ulcers. With the activation, alternation and overlap of one or more factors, the body's immunity decreases and immune function is disordered, which also causes frequent attacks of oral ulcers. There is still no specific method to cure oral ulcers. The principle of treatment is to eliminate the cause, strengthen the physical fitness, and treat symptoms to reduce the number of recurrences, prolong the interval period, relieve pain and promote healing. Treatment advocates the combination of systemic and local, traditional Chinese medicine and Western medicine, physiology and psychology. Systemic treatment generally uses immunosuppressants, immunomodulators, immunopotentiators, etc. to enhance the patient's own immunity, so that the patient's ulcers can heal slowly. Commonly used drugs are prednisone (prednisone), vitamin C, vitamin B complex and other drugs. Local treatment mainly relies on western medicine, such as 0.1% triamcinolone, glucocorticoids, immunosuppressants, lidocaine gel, etc. However, some western medicines have serious toxic side effects, and generally only some drugs such as watermelon frost and ice pen powder can be used to relieve the pain of the affected area. However, according to clinical reactions, most patients believe that the current drug treatment effect is still not ideal. Therefore, it is urgent to find a suitable treatment drug. Summary of the invention

[0003] The technical problem to be solved by the present invention is to provide an oral ulcer membrane containing multiple plant extracts and a preparation method thereof in view of the deficiencies of the above-mentioned prior art. The oral ulcer membrane can improve the problem of oral inflammation and has a good analgesic and detumescent effect.

[0004] In order to solve the above technical problems, the technical solution adopted by the present invention is: an oral ulcer membrane containing multiple plant extracts, which is prepared by drying the following raw materials in percentage by mass: 0.1% to 1.5% of licorice chalcone extract, 0.25% to 0.5% of reed root extract, 0.25% to 0.5% of Cistanche extract, 0.25% to 0.5% of rose extract, 2.58% of polyvinyl alcohol, 0.28% of sodium carboxymethyl cellulose, 0.46% of glycerol, and the balance is water.

[0005] The present invention also provides a method for preparing the above-mentioned oral ulcer film containing multiple plant extracts, which method comprises:

[0006] S1. Preparation of licorice chalcone extract: wash and dry licorice, add water a to the dried licorice, heat and boil, reduce the temperature to 90°C-100°C and keep warm for 1h, perform the first extraction, filter with filter cloth after extraction to obtain filtrate a, add water b to the filtered licorice residue, boil again, reduce the temperature to 90°C-100°C and keep warm for 1h, perform the second extraction, filter with filter cloth after extraction to obtain filtrate b, dry the obtained licorice residue to obtain dried licorice residue, add 60% ethanol solution by mass to soak for 30min, heat to boiling, reduce the temperature to 80°C-90°C and keep warm for 1.5h, filter with filter cloth after extraction to obtain filtrate c, dilute the filtrate c and pass it through D101 macroporous resin, elute with 80% ethanol solution by mass, concentrate and dry to obtain licorice chalcone extract;

[0007] S2, preparation of reed root extract: after washing and drying the reed root, water a is added to the dried reed root, heated and boiled, and the temperature is reduced to 90°C to 100°C and kept warm for 1h, a first extraction is performed, and the extraction is filtered with a filter cloth to obtain a filtrate a, water b is added to the filtered reed root residue a, the mixture is boiled again, and the temperature is reduced to 90°C to 100°C and kept warm for 1h, a second extraction is performed, and the extraction is filtered with a filter cloth to obtain a filtrate b, water c is added to the filtered reed root residue b, the mixture is boiled again, and the temperature is reduced to 90°C to 100°C and kept warm for 1h, a third extraction is performed, and the extraction is filtered with a filter cloth to obtain a filtrate c, the filtrate a, the filtrate b and the filtrate c are mixed, and the mixture is concentrated under reduced pressure to a clear paste with a relative density of 1.08 to 1.15, and the mixture is spray-dried, crushed and sieved to obtain a reed root extract;

[0008] S3. Preparation of Cistanche deserticola extract: Wash and dry Cistanche deserticola, add 60% ethanol solution a by mass to the dried Cistanche deserticola, keep warm at 75°C to 80°C for 1.5 hours, perform the first extraction, filter with filter cloth after extraction to obtain filtrate a, add 60% ethanol solution b by mass to the filtered Cistanche deserticola residue a, keep warm at 75°C to 80°C for 1.5 hours, perform the second extraction , after extraction, filter with filter cloth to obtain filtrate b, add 60% ethanol solution c by mass to the filtered Cistanche residue b, keep warm at 75°C to 80°C for 1.5h, perform a third extraction, filter with filter cloth after extraction to obtain filtrate c, mix the filtrate a, filtrate b and filtrate c, concentrate under reduced pressure to a clear paste with a relative density of 1.05 to 1.15, spray dry, crush and sieve to obtain Cistanche extract;

[0009] S4, preparation of rose extract: adding water a to Hotan rose, heating and boiling, keeping the temperature at 90°C to 100°C for 1.5 hours, performing the first extraction, filtering with filter cloth after extraction to obtain filtrate a, adding water b to the filtered rose residue a, boiling again, keeping the temperature at 90°C to 100°C for 1 hour, performing the second extraction, filtering with filter cloth after extraction to obtain filtrate b, adding water c to the filtered rose residue b, boiling again, keeping the temperature at 90°C to 100°C for 1 hour, performing the third extraction, filtering with filter cloth after extraction to obtain filtrate c, mixing the filtrate a, filtrate b and filtrate c, concentrating under reduced pressure to a clear paste with a relative density of 1.08 to 1.15, spray drying, crushing and sieving to obtain rose extract;

[0010] S5, adding polyvinyl alcohol and sodium carboxymethyl cellulose into water, soaking and swelling, and heating at 80° C. to remove bubbles, to obtain a mixture A;

[0011] S6, adding glycerin, the licorice chalcone extract obtained in S1, the reed root extract obtained in S2, the Cistanche deserticola extract obtained in S3 and the rose extract obtained in S4 to the mixture A obtained in S5, stirring and mixing evenly to obtain a mixture B;

[0012] S7. Apply the uniform solution of the mixture B obtained in S6 onto the cleaned glass, and after drying, obtain an oral ulcer film containing multiple plant extracts.

[0013] Preferably, in S1, the mass ratio of the dried licorice to water a is 1:10; the mass ratio of the filtered licorice residue to water b is 1:8; the mass ratio of the dried licorice residue to 60% ethanol solution is 1:10.

[0014] Preferably, in S2, the mass ratio of the dried reed root and water a is 1:10; the mass ratio of the filtered reed root residue a and water b is 1:8; and the mass ratio of the filtered reed root residue b and water c is 1:8.

[0015] Preferably, in S3, the mass ratio of the dried Cistanche deserticola to the 60% ethanol solution a is 1:8; the mass ratio of the filtered Cistanche deserticola residue a to the 60% ethanol solution b is 1:8; the mass ratio of the filtered Cistanche deserticola residue b to the 60% ethanol solution c is 1:8.

[0016] Preferably, the mass ratio of the Hotan rose and water a in S4 is 1:12; the mass ratio of the filtered rose residue a and water b is 1:10; the mass ratio of the filtered rose residue b and water c is 1:10.

[0017] Compared with the prior art, the present invention has the following advantages:

[0018] 1. The licorice chalcone extract used in the present invention can not only promote the healing of oral ulcer wounds, but also significantly reduce the concentration of inflammatory factors, and has a good oral ulcer recovery effect; the reed root extract contains a variety of antibacterial and anti-inflammatory ingredients, which has analgesic and detumescent effects; the Cistanche extract and the rose extract have good anti-inflammatory and analgesic effects. The four plant extracts all have antibacterial and anti-inflammatory effects, wherein the licorice extract also has a good anti-ulcer effect. The prepared oral care product can improve the problem of oral inflammation, has a good analgesic and detumescent effect, and achieves the effects of anti-inflammatory, antibacterial and treatment of oral ulcers. The oral ulcer film used is a relatively small film, which contains four natural plant extracts. It is mainly based on Chinese herbal medicine ingredients, does not contain hormones and antibiotics, etc., so there is no need to worry about toxic side effects and drug resistance. After being attached to the affected part of the ulcer, the drug ingredients can be quickly absorbed by the skin, thereby alleviating and treating the oral ulcer phenomenon. The oral ulcer membrane is used externally, so it will not irritate the stomach, nor will it harm the liver and kidneys. After use, it can effectively help patients relieve pain, and it can also have a certain moisturizing effect on the mucous membrane around the oral ulcer.

[0019] 2. Compared with the currently used chemical fungicides with safety concerns, the natural plant extracts used in the present invention have active ingredients that are natural products of plant origin. They are safe to use, have no side effects, and have obvious effects. They have higher safety performance and are accepted by product users.

[0020] The present invention is further described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a three-dimensional graph of the film-forming response surface analysis of the amount of polyvinyl alcohol added and the amount of sodium carboxymethyl cellulose added in the molding parameter optimization experiment of Example 1 of the present invention.

[0022] Figure 2 It is a three-dimensional graph of the film-forming response surface analysis of the amount of polyvinyl alcohol added and the amount of glycerol added in the molding parameter optimization experiment of Example 1 of the present invention.

[0023] Figure 3 It is a three-dimensional graph of the film-forming response surface analysis of the addition amount of sodium carboxymethyl cellulose and the addition amount of glycerol in the molding parameter optimization experiment of Example 1 of the present invention.

[0024] Figure 4 It is a plane diagram of the film-forming response surface analysis of the amount of polyvinyl alcohol added and the amount of sodium carboxymethyl cellulose added in the molding parameter optimization experiment of Example 1 of the present invention.

[0025] Figure 5It is a plane diagram of the film-forming response surface analysis of the amount of polyvinyl alcohol added and the amount of glycerol added in the molding parameter optimization experiment of Example 1 of the present invention.

[0026] Figure 6 It is a plane diagram of the film-forming response surface analysis of the addition amount of sodium carboxymethyl cellulose and the addition amount of glycerol in the molding parameter optimization experiment of Example 1 of the present invention. DETAILED DESCRIPTION

[0027] Example 1

[0028] The oral ulcer membrane containing multiple plant extracts in this embodiment is made by drying the following raw materials in percentage by mass: 0.5% of licorice chalcone extract, 0.25% of reed root extract, 0.25% of Cistanche deserticola extract, 0.25% of rose extract, 2.58% of polyvinyl alcohol, 0.28% of sodium carboxymethyl cellulose, 0.46% of glycerol, and the remainder is water.

[0029] This embodiment also provides a method for preparing the above-mentioned oral ulcer film containing multiple plant extracts, which is:

[0030] S1. Preparation of licorice chalcone extract: Wash and dry licorice, add water a to the dried licorice, heat and boil, reduce the temperature to 90°C and keep warm for 1 hour, perform the first extraction, filter with filter cloth after extraction to obtain filtrate a, add water b to the filtered licorice residue, boil again, reduce the temperature to 90°C and keep warm for 1 hour, perform the second extraction, filter with filter cloth after extraction to obtain filtrate b, dry the obtained licorice residue to obtain the dried licorice residue, add 60% by mass ethyl acetate, The alcohol solution is soaked for 30 minutes, heated to boiling, and then kept warm for 1.5 hours when the temperature drops to 80°C. After extraction, it is filtered with a filter cloth to obtain a filtrate c, and the filtrate c is diluted and passed through a D101 macroporous resin, eluted with an ethanol solution with a mass fraction of 80%, concentrated and dried to obtain a licorice chalcone extract; the mass ratio of the dried licorice to water a is 1:10; the mass ratio of the filtered licorice residue to water b is 1:8; the mass ratio of the dried licorice residue to a 60% ethanol solution is 1:8;

[0031] S2. Preparation of reed root extract: After washing and drying the reed root, water a is added to the dried reed root, heated and boiled, and then the temperature is reduced to 90°C and kept warm for 1h, and the first extraction is performed, and after the extraction, it is filtered with a filter cloth to obtain a filtrate a, and water b is added to the filtered reed root residue a, and the mixture is boiled again, and the temperature is reduced to 90°C and kept warm for 1h, and the second extraction is performed, and after the extraction, it is filtered with a filter cloth to obtain a filtrate b, and water c is added to the filtered reed root residue b, and the mixture is boiled again, and the temperature is reduced to 90°C and kept warm for 1h, and the second extraction is performed, and the filtrate b is obtained. When the temperature drops to 90°C, the mixture is kept warm for 1 hour, and the third extraction is performed. After the extraction, the mixture is filtered with a filter cloth to obtain a filtrate c. The filtrate a, the filtrate b and the filtrate c are mixed, and then concentrated under reduced pressure to a clear paste with a relative density of 1.08. After spray drying, the mixture is crushed and sieved to obtain a rhizoma reed extract. The mass ratio of the dried rhizoma reed to water a is 1:10; the mass ratio of the filtered rhizoma reed residue a to water b is 1:8; the mass ratio of the filtered rhizoma reed residue b to water c is 1:8;

[0032] S3. Preparation of Cistanche deserticola extract: After washing and drying Cistanche deserticola, add 60% ethanol solution a by mass to the dried Cistanche deserticola, keep warm at 75°C to 80°C for 1.5 hours, perform the first extraction, filter with filter cloth after extraction to obtain filtrate a, add 60% ethanol solution b by mass to the filtered Cistanche deserticola residue a, keep warm at 75°C for 1.5 hours, perform the second extraction, filter with filter cloth after extraction to obtain filtrate b, add 60% ethanol solution c by mass to the filtered Cistanche deserticola residue b, keep warm at 75°C for 1.5 hours, perform the second extraction, filter with filter cloth after extraction to obtain filtrate b. The mixture is kept warm at 75°C for 1.5 hours, and the mixture is extracted for the third time. After the extraction, the mixture is filtered with a filter cloth to obtain a filtrate c. The filtrate a, the filtrate b and the filtrate c are mixed, and the mixture is concentrated under reduced pressure to a clear paste with a relative density of 1.05. After spray drying, the mixture is crushed and sieved to obtain a Cistanche extract. The mass ratio of the dried Cistanche to the 60% ethanol solution a is 1:8. The mass ratio of the filtered Cistanche residue a to the 60% ethanol solution b is 1:8. The mass ratio of the filtered Cistanche residue b to the 60% ethanol solution c is 1:8.

[0033] S4, preparation of rose extract: add water a to Hotan rose, heat and boil, keep warm for 1.5 hours when the temperature drops to 90°C, perform the first extraction, filter with filter cloth after extraction to obtain filtrate a, add water b to the filtered rose residue a, boil again, keep warm for 1 hour when the temperature drops to 90°C, perform the second extraction, filter with filter cloth after extraction to obtain filtrate b, add water c to the filtered rose residue b, boil again, keep warm for 1 hour when the temperature drops to 90°C, perform the third extraction, filter with filter cloth after extraction to obtain filtrate c, mix the filtrate a, filtrate b and filtrate c, concentrate under reduced pressure to a clear paste with a relative density of 1.08, spray dry, crush and sieve to obtain rose extract; the mass ratio of the Hotan rose to water a is 1:12; the mass ratio of the filtered rose residue a and water b is 1:10; the mass ratio of the filtered rose residue b and water c is 1:10;

[0034] S5, adding polyvinyl alcohol and sodium carboxymethyl cellulose into water, soaking and swelling, and heating at 80° C. to remove bubbles, to obtain a mixture A;

[0035] S6, adding glycerin, the licorice chalcone extract obtained in S1, the reed root extract obtained in S2, the Cistanche deserticola extract obtained in S3 and the rose extract obtained in S4 to the mixture A obtained in S5, stirring and mixing evenly to obtain a mixture B;

[0036] S7. Apply the uniform solution of the mixture B obtained in S6 onto the cleaned glass, and after drying, obtain an oral ulcer film containing multiple plant extracts.

[0037] In order to verify the film-forming effect of the oral ulcer film of this embodiment, experiments related to the film-forming property of the film agent were first carried out. The amount of polyvinyl alcohol added, the amount of sodium carboxymethyl cellulose added, and the amount of glycerol added were mainly used as the main influencing factors, and the comprehensive score (film-forming property, appearance, toughness) was used as the evaluation index. The molding parameter optimization test was carried out (the preliminary verification results showed that the addition of various extracts had little effect on the film-forming property after the film-forming agent was prepared. Due to the different types and amounts of extracts added, they are not listed as the main influencing factors of film-forming property).

[0038] Test 1

[0039] Box-Behnken response surface optimization experiment

[0040] Based on the previous experiments, the amount of polyvinyl alcohol added (A), sodium carboxymethyl cellulose added (B), and glycerol added (C) were used as influencing factors, and the comprehensive score (film-forming property, appearance, and toughness) was used as the evaluation index. The Box-Behnken response surface method was used to optimize the preparation process. The scoring criteria are as follows. The experimental design is shown in Table 1.

[0041] Table 1 Response surface design factors and levels

[0042]

[0043] Scoring Criteria

[0044] Evaluation factors of oral ulcer film include film formation, appearance and toughness.

[0045] (1) Film-forming property - evaluated based on film-forming time and the ease of film removal. The shorter the film-forming time and the more complete the film is, the higher the score is, with a full score of 10 points. Specifically, if the film-forming time is less than 60 minutes and the film is completely removed, the score is 10 points; if the film-forming time is 70-80 minutes and there are 1-2 cracks in the film-removing process, the score is 8-9 points; if the film-forming time is 90-100 minutes, some of the film-forming agent (less than 30% of the total area) is difficult to remove during the film-removing process, the score is 6-7 points; if the film-forming time is 110-120 minutes, some of the film-forming agent (about 30%-50% of the total area) is difficult to remove during the film-removing process, the score is 4-5 points; if the film-forming time is 120-130 minutes, most of the film-forming agent (greater than 50% of the total area) is difficult to remove during the film-removing process, the score is 0.

[0046] (2) Appearance - 10 volunteers were randomly selected to evaluate the film by visual inspection, touching the film surface, and observing the amount of bubbles, thickness, and color, with a full score of 10. Specifically, the film has uniform color, smooth surface, and no bubbles, with a score of 10; the film has uniform color, smooth surface, and 1 to 10 bubbles, with a score of 9 to 3 points; the film has uniform color, rough surface, and a small number of bubbles (11 to 24), with a score of 2 points; the film has uneven color, rough surface, and a large number of bubbles (≥25), with a score of 1 point; the film is not formed, with a score of 0 points. The average of the scores of the 10 volunteers is taken as the final appearance score.

[0047] (3) Toughness - The folding resistance (folding times) is used to evaluate the softness of the film. The same size of film (1 cm × 1 cm) is folded repeatedly. The more times the film is folded without damage, the higher the score. The full score is 10. Specifically, if it can be folded 6 times, the score is 10 points; if it can be folded 5 times, the score is 9 points; if it can be folded 4 times, the score is 8 points; if it can be folded 3 times, the score is 7 points; if it can be folded less than 2 times, the score is 4-6; if it can be folded 0 times, the score is 0 points.

[0048] (I) Test results

[0049] The experimental data were fitted and analyzed using Design-Expert software, and a quadratic regression model was obtained between the comprehensive score of film forming and the amount of polyvinyl alcohol added, the amount of sodium carboxymethyl cellulose added, and the amount of glycerol added:

[0050] Y=94.28-0.57A-0.94B-1.01C+0.22AB+0.27AC-0.05BC-2.59A 2 -3.02B 2 -2.81C 2 .

[0051] The factor results are shown in Table 2.

[0052] Table 2 Response surface design scheme and experimental results factors

[0053] Test No. A polyvinyl alcohol addition amount / g B. Addition amount of sodium carboxymethyl cellulose / g C Glycerol addition amount / g Y comprehensive score 1 2.7 0.2 0.7 88.5 2 3.6 0.2 0.5 88.6 3 2.7 0.3 0.5 94.3 4 3.6 0.3 0.3 89.3 5 1.8 0.3 0.7 87.9 6 2.7 0.2 0.3 90.3 7 2.7 0.4 0.7 86.5 8 1.8 0.3 0.3 90.6 9 3.6 0.4 0.5 87.2 10 3.6 0.3 0.7 87.7 11 2.7 0.3 0.5 94.5 12 1.8 0.2 0.5 90.6 13 1.8 0.4 0.5 88.3 14 2.7 0.4 0.3 88.5 15 2.7 0.3 0.5 94.2 16 2.7 0.3 0.5 94.1 17 2.7 0.3 0.5 94.3

[0054] The results of variance analysis are shown in Table 3.

[0055] Table 3 Results of variance analysis

[0056]

[0057]

[0058] Note: * indicates significant difference (P<0.05); ** indicates extremely significant difference (P<0.01).

[0059] The results of variance analysis are shown in Table 3. The significance test F value of the regression model for the comprehensive score of oral ulcer film formation is 229.47, P < 0.0001, indicating that the regression model has reached an extremely significant level; the F value of the lack of fit test is 5.34, P = 0.0696 > 0.05, and the lack of fit test is not significant, indicating that the model is established and can be used to predict the effects of various factors of the formula on the film forming results. Model correlation coefficient R 2 =0.9966, model adjusted correlation coefficient R 2 Adj =0.9923, indicating that 0.77% of the response value changes in this model cannot be explained. 2 , B 2 , C 2 The first term A was significant. Comparison of the F values ​​showed that the factors affecting the film forming results were arranged in the order of C>B>A. Through response surface optimization analysis, the optimal parameters for oral ulcer film forming were: polyvinyl alcohol addition of 2.58g, sodium carboxymethyl cellulose addition of 0.28g, and glycerol addition of 0.46g, and the comprehensive score prediction value was 94.5.

[0060] (II) Response surface plot analysis

[0061] The steepness of the response surface can reflect the influence of various factors on the comprehensive score of the formation of oral ulcer film. The greater the steepness of the surface, the more significant the interaction between the two factors. Figure 1-6 As shown in the figure, the comprehensive score of the formation of oral ulcer membrane first increased and then decreased with the increase of each factor. The response surface had a moderate steepness and the contour line shape was circular, indicating that the interaction between the factors was not significant, which was consistent with the results of variance analysis.

[0062] (III) Molding parameter optimization and verification experiment

[0063] Through response surface optimization analysis, the optimal parameters of oral ulcer film were obtained as follows: 2.58g of polyvinyl alcohol, 0.28g of sodium carboxymethyl cellulose, and 0.46g of glycerol. Under this optimized condition, the predicted value of the comprehensive score of oral ulcer film formation was 94.5. The final process was to weigh 2.58g of polyvinyl alcohol and 0.28g of sodium carboxymethyl cellulose as film-forming materials, soak them in water overnight to fully swell, heat to remove bubbles, add 0.46g of glycerol, and a mixture of extracts (including 0.5g of licorice chalcone extract, 0.25g of rhizome of reed, 0.25g of Cistanche extract, and 0.25g of rose extract) and stir carefully until uniform, apply it to the cleaned glass, and dry it to obtain an oral ulcer film. Three batches of verification tests were carried out, and the comprehensive scores of the film-forming property, appearance, and toughness of the film were 94.5, 94.6, and 94.5, respectively, which were close to the predicted values ​​and met the relevant standards.

[0064] (IV) Determination of film weight difference

[0065] Using the optimal ratio formula, 20 pieces of 1 cm × 1 cm oral ulcer membranes were prepared, and the total weight of the membrane was accurately weighed. After averaging the weight of each piece, each piece was accurately weighed and the weight difference was calculated. The results are shown in Table 4.

[0066] The calculation formula is: Weight difference = (single piece weight - average weight) / average weight × 100%

[0067] Table 4 Oral ulcer membrane weight difference determination

[0068]

[0069] According to the test results, the weight difference of each piece of oral ulcer membrane is within ±7.5%, which meets the requirements of the Chinese Pharmacopoeia (2020 edition) for film preparations.

[0070] (V) Determination of film dissolution time

[0071] Six oral ulcer membranes prepared using the best method were evenly attached to the wall of a 500 mL beaker containing 200 mL of distilled water, 3 cm from the water surface, at a temperature of 37°C. The complete dissolution time after attachment was recorded, and the average complete dissolution time was calculated. The results are shown in Table 5.

[0072] Table 5 Oral ulcer membrane dissolution time determination

[0073] project 1 2 3 4 5 6 average value Dissolution time / min 63 62 66 67 71 62 65.17

[0074] The test results showed that the average dissolution time of oral ulcer membrane was 65.17 minutes.

[0075] Test 2

[0076] This experiment is a systematic experiment on the oral ulcer membrane against glacial acetic acid-induced oral ulcer model in SD rats.

[0077] (1) Establishment of traumatic oral ulcer model

[0078] The SD rats that needed to be modeled were anesthetized and placed sideways on the animal operating table. The labial mucosa of the rats was dried with absorbent cotton balls and kept wet with sterile cotton balls. The absorbent cotton was inserted into a 1mL syringe (the lower end was cut into an inner diameter of 5mm) and kept flush with the tube mouth, and immersed in 50% glacial acetic acid solution. The front end of the syringe soaked with absorbent cotton balls was vertically pressed on the mucosal surface of the left and right sides of the rat and 1mm away from the corner of the mouth. The effect lasted for 60s, and the local area turned grayish white. The formation of the ulcer surface was observed after 24 hours.

[0079] (2) Experimental Grouping and Drug Administration Before modeling, the rats were randomly divided into 6 groups (10 rats in each group) according to their gender and body weight. The groups are shown in Table 6.

[0080] ① Watermelon frost group (positive control group): 15 mg of watermelon frost was administered to each side, with medication given to both sides.

[0081] ② Oral ulcer film: Cut the prepared oral ulcer film into 5mm×5mm square pieces and stick them on the ulcer when using. According to the number of administrations, they are divided into high, medium and low dose groups of oral ulcer film and administered as follows:

[0082] a. Oral ulcer film high-dose group: The oral ulcer film was applied to the buccal mucosal ulcer of rats 4 times a day with an interval of 2 hours between each application.

[0083] b. Oral ulcer film medium dose group: The oral ulcer film was applied to the buccal mucosal ulcer of rats twice a day with an interval of 2 hours between each application.

[0084] c. Oral ulcer film low-dose group: The oral ulcer film was applied to the buccal mucosal ulcer of rats once a day.

[0085] ③Model group: an ulcer model was created, and then the ulcer surface was only rinsed with saline, and no therapeutic drugs were given.

[0086] ④Blank control group: ordinary rats without oral ulcers, without the establishment of oral ulcer model.

[0087] The model group and blank control group were given the same volume of normal saline.

[0088] The drug was administered continuously for 3 days.

[0089] (3) Rat handling and sampling methods

[0090] The rats were fasted but not watered for 12 h. After anesthesia, they were fixed on the operating table. Blood was drawn from the abdominal aorta. The animals were killed and the ulcer tissues on the inner cheeks of the rats were removed and divided into two parts. One part was used for homogenization and the other part was sealed with tin foil and stored in a -80℃ refrigerator for later use.

[0091] (4) Determination of ulcer area

[0092] The ulcer area before administration and on day 3 after administration were measured. The rat's mouth was opened with hemostatic forceps to expose the ulcer surface, and the ulcer surface was photographed and recorded with an oral endoscope, and the area was calculated using ImageJ software.

[0093] (5) Appearance evaluation

[0094] The ulcer surface was divided into three levels and scored: Grade I had no obvious pseudomembrane on the surface, which was scored as 1 point; Grade II had a thin layer of yellow-white pseudomembrane on the surface of the ulcer, with no surrounding edema, which was scored as 2 points; Grade III had a thicker pseudomembrane on the surface of the ulcer, with surrounding edema, which was scored as 3 points. The control group had no ulcer surface and was scored as 0.

[0095] (6) Determination of TNF-α and IL-1β content in local tissues of rats

[0096] The experiment used a rat TNF-α enzyme-linked immunosorbent assay kit to determine the concentration of TNF-α in rat local tissue samples; and a rat IL-1β enzyme-linked immunosorbent assay kit was used to determine the concentration of IL-1β in rat local tissue samples.

[0097] (7) Modeling results

[0098] On the second day of modeling, the rats showed reduced activity, messy fur, drooling and wet lips, and an ulcer of about 25 mm formed in the modeling area. The surrounding area showed varying degrees of congestion and redness and swelling. The concave surface of the ulcer was covered with a yellow or yellow-white pseudomembrane, indicating that the modeling was successful.

[0099] (8) Effect of oral ulcer film on body weight of rats with oral ulcer

[0100] After the administration, the body weight of the rats in the model group decreased, which was statistically significant compared with the blank control group (P<0.01), and the body weight of the rats in the positive control group and the oral ulcer membrane groups increased, but there was no statistical significance compared with the model group (P>0.05). The results are shown in Table 6 below.

[0101] Table 6 Animal grouping, medication regimen and the effect of oral ulcer membrane on rat body weight ( n=10)

[0102]

[0103] Note: Compared with the blank control group, ** indicates significant difference at the P<0.01 level.

[0104] (9) Effects of oral ulcer film on the area and surface condition of oral ulcers in rats

[0105] Before administration, there was no statistically significant difference in the oral ulcer area between the rats in each administration group (including the positive control group and the low-, medium- and high-dose groups of oral ulcer membrane) and the model group (P>0.05). After administration, the oral ulcer area of ​​the rats in the positive control group and each dose group of oral ulcer membrane was significantly reduced, which was statistically significant compared with the model group (P<0.01).

[0106] Before administration, thick pseudomembranes appeared on the surface of oral ulcers in rats of each modeling group (including positive control group, model group and oral ulcer membrane low, medium and high dose groups), and edema was present around them; after administration, pseudomembranes were still visible on the surface of oral ulcers in rats of model group, and edema was present around them, while the surface of oral ulcers in rats of positive control group and oral ulcer membrane groups improved, and the scores were all lower than those in model group (P<0.01). The results are shown in Table 7.

[0107] Table 7 Effect of oral ulcer film on the area and surface condition of oral ulcer in rats ( n=10)

[0108]

[0109] Note: Compared with the blank control group, ** indicates a significant difference at the P<0.01 level; compared with the model group, ## Indicates significant difference at the P<0.01 level. # Indicates significant difference at the P<0.05 level.

[0110] Oral ulcer membrane can promote the healing of oral ulcers in rats. The ulcer area was measured, and the results of the ulcer area of ​​each group showed that there was no statistical significance in the size of the ulcer area of ​​each modeling group before administration (P < 0.05); 3 days after administration, the ulcer area and degree of the positive control group and each dose group of oral ulcer membrane were lower than those of the model group, and the difference was statistically significant (P < 0.05), indicating that each dose group of oral ulcer membrane can promote the healing of rat ulcer surface and accelerate its healing rate.

[0111] (10) Effects of oral ulcer membrane on inflammatory factors in local ulcer tissue

[0112] After administration, the TNF-α level in the oral ulcer tissue of the rats in the model group increased, which was statistically significant compared with the blank control group (P<0.05). The TNF-α level in the oral ulcer tissue of the rats in each administration group decreased, and the TNF-α content in the oral ulcer tissue of the rats in the positive control group and the oral ulcer membrane high-dose group was statistically significant compared with the model group (P<0.05, P<0.01). The IL-1β level in the oral ulcer tissue of the rats in the model group increased, but there was no statistical significance compared with the blank control group. The results are shown in Table 8.

[0113] Table 8 Effect of oral ulcer membrane on inflammatory factors in local ulcer tissue ( n=8)

[0114]

[0115] Note: Compared with the blank control group, ** indicates a significant difference at the P<0.01 level; compared with the model group, ## Indicates significant difference at the P<0.01 level. # Indicates significant difference at the P<0.05 level.

[0116] Oral ulcer membrane can reduce the concentration of inflammatory factors. A large number of clinical and experimental studies have confirmed that during the course of oral ulcers, abnormally activated macrophages in the oral mucosa secrete a large number of proinflammatory factors (such as TNF-α, IL-1β, etc.) that directly damage the mucosa and surrounding tissues, and can also activate the NF-κB inhibitory protein kinase complex, promote NF-κB translocation to the nucleus, and then bind to the NF-κB site on the target gene promoter region or enhancer, start the expression of proinflammatory factors such as TNF-α and IL-1β, inhibit the gene expression of anti-inflammatory factors such as IL-4 and IL-10, increase the synthesis and secretion of TNF-α, IL-1β, etc., and make the balance between anti-inflammatory factors and proinflammatory factors lose balance, aggravate oral mucosal damage, which is also an important reason for oral ulcers to be lingering and difficult to heal. In this experiment, the concentrations of inflammatory factors TNF-α and IL-1β in the local oral tissues of rats measured were significantly different in the high-dose group of oral ulcer membrane compared with the model group, which also directly shows that the oral ulcer membrane has the effect of reducing the concentration of inflammatory factors.

[0117] Example 2

[0118] The oral ulcer membrane containing multiple plant extracts in this embodiment is made by drying the following raw materials in percentage by mass: 0.1% of licorice chalcone extract, 0.5% of reed root extract, 0.5% of Cistanche extract, 0.5% of rose extract, 2.58% of polyvinyl alcohol, 0.28% of sodium carboxymethyl cellulose, 0.46% of glycerol, and the remainder is water.

[0119] The method of preparing the oral ulcer membrane containing various plant extracts in this embodiment is the same as that in embodiment 1.

[0120] Example 3

[0121] The oral ulcer membrane containing multiple plant extracts in this embodiment is made by drying the following raw materials in percentage by mass: 1.5% of licorice chalcone extract, 0.4% of reed root extract, 0.3% of Cistanche extract, 0.4% of rose extract, 2.58% of polyvinyl alcohol, 0.28% of sodium carboxymethyl cellulose, 0.46% of glycerol, and the remainder is water.

[0122] The method of preparing the oral ulcer membrane containing various plant extracts in this embodiment is the same as that in embodiment 1.

[0123] Comparative Example 1

[0124] The oral ulcer membrane of this embodiment is prepared by drying the following raw materials in percentage by mass: 0.5% of licorice chalcone extract, 2.58% of polyvinyl alcohol, 0.28% of sodium carboxymethyl cellulose, 0.46% of glycerol, and the remainder is water.

[0125] The method of the oral ulcer film of this comparative example is:

[0126] S1. Preparation of licorice chalcone extract: Wash and dry licorice, add water a to the dried licorice, heat and boil, reduce the temperature to 90°C and keep warm for 1 hour, perform the first extraction, filter with filter cloth after extraction to obtain filtrate a, add water b to the filtered licorice residue, boil again, reduce the temperature to 90°C and keep warm for 1 hour, perform the second extraction, filter with filter cloth after extraction to obtain filtrate b, dry the obtained licorice residue to obtain the dried licorice residue, add 60% by mass ethyl acetate, The alcohol solution is soaked for 30 minutes, heated to boiling, and then kept warm for 1.5 hours when the temperature drops to 80°C. After extraction, it is filtered with a filter cloth to obtain a filtrate c, and the filtrate c is diluted and passed through a D101 macroporous resin, eluted with an ethanol solution with a mass fraction of 80%, concentrated and dried to obtain a licorice chalcone extract; the mass ratio of the dried licorice to water a is 1:10; the mass ratio of the filtered licorice residue to water b is 1:8; the mass ratio of the dried licorice residue to a 60% ethanol solution is 1:8;

[0127] S2, adding polyvinyl alcohol and sodium carboxymethyl cellulose into water, soaking and swelling, and heating at 80° C. to remove bubbles, to obtain a mixture A;

[0128] S3, adding glycerin and the licorice chalcone extract obtained in S1 to the mixture A obtained in S2, stirring and mixing evenly to obtain a mixture B;

[0129] S4. Apply the uniform solution of the mixture B obtained in S3 onto the cleaned glass, and after drying, obtain an oral ulcer film.

[0130] Comparative Example 2

[0131] The oral ulcer membrane of this embodiment is made by drying the following raw materials in percentage by mass: 0.25% of rhizoma reed extract, 0.25% of cistanche extract, 0.25% of rose extract, 2.58% of polyvinyl alcohol, 0.28% of sodium carboxymethyl cellulose, 0.46% of glycerol, and the remainder is water.

[0132] The preparation method of the oral ulcer film of the present embodiment is:

[0133] S1. Preparation of rhizoma reed extract: After washing and drying the rhizoma reed, water a is added to the dried rhizoma reed, heated and boiled, and then the temperature is reduced to 90°C and kept warm for 1 hour for the first extraction, and then filtered with filter cloth to obtain filtrate a, and water b is added to the filtered rhizoma reed residue a, and the mixture is boiled again, and the temperature is reduced to 90°C and kept warm for 1 hour for the second extraction, and then filtered with filter cloth to obtain filtrate b, and water c is added to the filtered rhizoma reed residue b, and the mixture is boiled again, and the temperature is reduced to 90°C and kept warm for 1 hour for the second extraction, and then filtered with filter cloth to obtain filtrate b. When the temperature drops to 90°C, the mixture is kept warm for 1 hour, and the third extraction is performed. After the extraction, the mixture is filtered with a filter cloth to obtain a filtrate c. The filtrate a, the filtrate b and the filtrate c are mixed, and then concentrated under reduced pressure to a clear paste with a relative density of 1.08. After spray drying, the mixture is crushed and sieved to obtain a rhizoma reed extract. The mass ratio of the dried rhizoma reed to water a is 1:10; the mass ratio of the filtered rhizoma reed residue a to water b is 1:8; the mass ratio of the filtered rhizoma reed residue b to water c is 1:8;

[0134] S2. Preparation of Cistanche deserticola extract: After washing and drying Cistanche deserticola, add 60% ethanol solution a by mass to the dried Cistanche deserticola, keep warm at 75°C to 80°C for 1.5 hours, perform the first extraction, filter with filter cloth after extraction to obtain filtrate a, add 60% ethanol solution b by mass to the filtered Cistanche deserticola residue a, keep warm at 75°C for 1.5 hours, perform the second extraction, filter with filter cloth after extraction to obtain filtrate b, add 60% ethanol solution c by mass to the filtered Cistanche deserticola residue b, keep warm at 75°C for 1.5 hours, perform the second extraction, filter with filter cloth after extraction to obtain filtrate b. The mixture is kept warm at 75°C for 1.5 hours, and the mixture is extracted for the third time. After the extraction, the mixture is filtered with a filter cloth to obtain a filtrate c. The filtrate a, the filtrate b and the filtrate c are mixed, and the mixture is concentrated under reduced pressure to a clear paste with a relative density of 1.05. After spray drying, the mixture is crushed and sieved to obtain a Cistanche extract. The mass ratio of the dried Cistanche to the 60% ethanol solution a is 1:8. The mass ratio of the filtered Cistanche residue a to the 60% ethanol solution b is 1:8. The mass ratio of the filtered Cistanche residue b to the 60% ethanol solution c is 1:8.

[0135] S3, preparation of rose extract: add water a to Hotan rose, heat and boil, keep warm for 1.5 hours when the temperature drops to 90°C, perform the first extraction, filter with filter cloth after extraction to obtain filtrate a, add water b to the filtered rose residue a, boil again, keep warm for 1 hour when the temperature drops to 90°C, perform the second extraction, filter with filter cloth after extraction to obtain filtrate b, add water c to the filtered rose residue b, boil again, keep warm for 1 hour when the temperature drops to 90°C, perform the third extraction, filter with filter cloth after extraction to obtain filtrate c, mix the filtrate a, filtrate b and filtrate c, concentrate under reduced pressure to a clear paste with a relative density of 1.08, spray dry, crush and sieve to obtain rose extract; the mass ratio of the Hotan rose to water a is 1:12; the mass ratio of the filtered rose residue a and water b is 1:10; the mass ratio of the filtered rose residue b and water c is 1:10;

[0136] S4, adding polyvinyl alcohol and sodium carboxymethyl cellulose into water, soaking and swelling, and heating at 80° C. to remove bubbles, to obtain a mixture A;

[0137] S5, adding glycerin, the rhizome of Phragmites australis extract obtained in S1, the Cistanche deserticola extract obtained in S2, and the rose extract obtained in S3 to the mixture A obtained in S4, stirring and mixing evenly to obtain a mixture B;

[0138] S6. Apply the uniform solution of the mixture B obtained in S5 onto the cleaned glass, and after drying, obtain an oral ulcer film.

[0139] Comparative Example 3

[0140] The comparative example of the present invention is a blank oral ulcer film: 2.58% polyvinyl alcohol, 0.28% sodium carboxymethyl cellulose, 0.46% glycerol, and the balance is water.

[0141] The preparation method is:

[0142] S1, adding polyvinyl alcohol and sodium carboxymethyl cellulose into water, soaking and swelling, and heating at 80° C. to remove bubbles, to obtain a mixture A;

[0143] S2, adding glycerol to the mixture A obtained in S1, stirring and mixing evenly to obtain a mixture B;

[0144] S3. Apply the uniform solution of the mixture B obtained in S2 onto the cleaned glass, and after drying, obtain a blank oral ulcer film.

[0145] The oral ulcer membrane was used to verify the oral ulcer model induced by glacial acetic acid in SD rats.

[0146] (1) Establishment of traumatic oral ulcer model

[0147] The SD rats that needed to be modeled were anesthetized and placed sideways on the animal operating table. The labial mucosa of the rats was dried with absorbent cotton balls and kept wet with sterile cotton balls. The absorbent cotton was inserted into a 1mL syringe (the lower end was cut into an inner diameter of 5mm) and kept flush with the tube mouth, and immersed in 50% glacial acetic acid solution. The front end of the syringe soaked with absorbent cotton balls was vertically pressed on the mucosal surface of the left and right sides of the rat and 1mm away from the corner of the mouth. The effect lasted for 60s, and the local area turned grayish white. The formation of the ulcer surface was observed after 24 hours.

[0148] (2) Experimental groups and drug administration

[0149] Before modeling, the rats were randomly divided into 7 groups (10 rats in each group) according to their gender and weight, and the experiments of Examples 1-3, Comparative Examples 1-3, the model group and the blank control group were carried out respectively.

[0150] Embodiment 1-3, comparative example 1-3:

[0151] The oral ulcer films prepared according to the methods of Examples 1-3 and Comparative Examples 1-3 were cut into 5 mm×5 mm sizes and applied to the buccal mucosal ulcers of rats 4 times a day with an interval of 2 hours between each application.

[0152] Model group: The ulcer surface was washed with normal saline once a day.

[0153] Blank control group: no ulcer surface, given the same volume of normal saline, rinse once a day.

[0154] (3) Rat handling and sampling methods

[0155] The rats were fasted but not watered for 12 h, fixed on the operating table after anesthesia, and then killed.

[0156] (4) Determination of ulcer area

[0157] The ulcer area before administration and on day 3 after administration were measured. The rat's mouth was opened with hemostatic forceps to expose the ulcer surface, and the ulcer surface was photographed and recorded with an oral endoscope, and the area was calculated using ImageJ software.

[0158] (5) Appearance evaluation

[0159] The ulcer surface was divided into three levels and scored: Grade I had no obvious pseudomembrane on the surface, which was scored as 1 point; Grade II had a thin layer of yellow-white pseudomembrane on the surface of the ulcer, with no surrounding edema, which was scored as 2 points; Grade III had a thicker pseudomembrane on the surface of the ulcer, with surrounding edema, which was scored as 3 points. The control group had no ulcer surface and was scored as 0.

[0160] (6) Modeling results

[0161] On the second day of modeling, the rats showed reduced activity, messy fur, drooling and wet lips, and an ulcer of about 25 mm formed in the modeling area. The surrounding area showed varying degrees of congestion and redness and swelling. The concave surface of the ulcer was covered with a yellow or yellow-white pseudomembrane, indicating that the modeling was successful.

[0162] (7) Effects of oral ulcer film on the area and surface condition of oral ulcers in rats

[0163] Before administration, there was no statistically significant difference in the oral ulcer area between the rats in each test group and the model group (P>0.05). After administration, the oral ulcer area of ​​the rats in the oral ulcer membrane Example 1 and 2 dose groups was significantly reduced, which was statistically significant compared with the model control group (P<0.01).

[0164] Before administration, thick pseudomembranes appeared on the surface of oral ulcers in rats of each administration group, and edema was present around them; after administration, pseudomembranes were still visible on the surface of oral ulcers in rats of the model group, and edema was present around them; the surface conditions of oral ulcers in rats of oral ulcer film embodiments 1, 2, and 3 groups were all improved, and embodiments 1 and 3 groups were better; the pseudomembranes on the surface of oral ulcers in rats of comparative example 1 were weakened; the pseudomembranes on the surface of oral ulcers in rats of comparative example 2 were not weakened, and the surrounding edema was relieved; there was no significant change in the blank film control of comparative example 3. The results are shown in Table 9.

[0165] Table 9 Effects of different embodiments on the area and surface condition of oral ulcers in rats ( n=10)

[0166]

[0167] Note: Compared with the blank control group, ** indicates a significant difference at the P<0.01 level; compared with the model group, ## Indicates significant difference at the P<0.01 level. # Indicates significant difference at the P<0.05 level.

[0168] It can be concluded from the above test results that the comparative example 3 (blank film) without adding 4 kinds of extracts has no significant effect on the oral ulcer area and surface conditions of rats, and the test results are similar to the model control group; the comparative example 1 with the addition of licorice chalcone extract alone can only alleviate the surface pseudomembrane of oral ulcers, while the surface pseudomembrane of oral ulcers of rats in the comparative example 2 without adding licorice chalcone extract is not weakened, and the surrounding edema is alleviated. And the test results of reducing licorice chalcone extract and increasing the other three kinds of extracts in Example 2 are slightly lower than those of Examples 1 and 3. It can be inferred that the licorice chalcone extract plays a major role in the process of treating the lesion of the oral ulcer membrane, and increasing the addition amount of licorice chalcone extract can further improve the oral ulcer area and surface conditions of rats, but the difference is not significant (comparison of the results of Examples 1 and 3). At the same time, the four kinds of plant extracts have the effects of synergistically cooperating to achieve the healing of oral ulcer wounds and anti-inflammatory, antibacterial, etc., which can greatly alleviate the pain and infection caused by oral ulcers and promote the healing of oral ulcers.

[0169] The above is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent change made to the above embodiment according to the technical essence of the invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. An oral ulcer membrane containing multiple plant extracts, characterized in that: The invention is prepared by drying the following raw materials in percentage by mass: 0.1%-1.5% of licorice chalcone extract, 0.25%-0.5% of reed root extract, 0.25%-0.5% of Cistanche deserticola extract, 0.25%-0.5% of rose extract, 2.58% of polyvinyl alcohol, 0.28% of sodium carboxymethyl cellulose, 0.46% of glycerol, and the balance is water.

2. A method for preparing the oral ulcer film containing multiple plant extracts as claimed in claim 1, characterized in that: The method is: S1. Preparation of licorice chalcone extract: wash and dry licorice, add water a to the dried licorice, heat and boil, reduce the temperature to 90°C-100°C and keep warm for 1h, perform the first extraction, filter with filter cloth after extraction to obtain filtrate a, add water b to the filtered licorice residue, boil again, reduce the temperature to 90°C-100°C and keep warm for 1h, perform the second extraction, filter with filter cloth after extraction to obtain filtrate b, dry the obtained licorice residue to obtain dried licorice residue, add 60% ethanol solution by mass to soak for 30min, heat to boiling, reduce the temperature to 80°C-90°C and keep warm for 1.5h, filter with filter cloth after extraction to obtain filtrate c, dilute the filtrate c and pass it through D101 macroporous resin, elute with 80% ethanol solution by mass, concentrate and dry to obtain licorice chalcone extract; S2, preparation of reed root extract: after washing and drying the reed root, water a is added to the dried reed root, heated and boiled, and the temperature is reduced to 90°C to 100°C and kept warm for 1h, a first extraction is performed, and the extraction is filtered with a filter cloth to obtain a filtrate a, water b is added to the filtered reed root residue a, the mixture is boiled again, and the temperature is reduced to 90°C to 100°C and kept warm for 1h, a second extraction is performed, and the extraction is filtered with a filter cloth to obtain a filtrate b, water c is added to the filtered reed root residue b, the mixture is boiled again, and the temperature is reduced to 90°C to 100°C and kept warm for 1h, a third extraction is performed, and the extraction is filtered with a filter cloth to obtain a filtrate c, the filtrate a, the filtrate b and the filtrate c are mixed, and the mixture is concentrated under reduced pressure to a clear paste with a relative density of 1.08 to 1.15, and the mixture is spray-dried, crushed and sieved to obtain a reed root extract; S3. Preparation of Cistanche deserticola extract: Wash and dry Cistanche deserticola, add 60% ethanol solution a by mass to the dried Cistanche deserticola, keep warm at 75°C to 80°C for 1.5 hours, perform the first extraction, filter with filter cloth after extraction to obtain filtrate a, add 60% ethanol solution b by mass to the filtered Cistanche deserticola residue a, keep warm at 75°C to 80°C for 1.5 hours, perform the second extraction , after extraction, filter with filter cloth to obtain filtrate b, add 60% ethanol solution c by mass to the filtered Cistanche residue b, keep warm at 75°C to 80°C for 1.5h, perform a third extraction, filter with filter cloth after extraction to obtain filtrate c, mix the filtrate a, filtrate b and filtrate c, concentrate under reduced pressure to a clear paste with a relative density of 1.05 to 1.15, spray dry, crush and sieve to obtain Cistanche extract; S4, preparation of rose extract: adding water a to Hotan rose, heating and boiling, keeping the temperature at 90°C to 100°C for 1.5 hours, performing the first extraction, filtering with filter cloth after extraction to obtain filtrate a, adding water b to the filtered rose residue a, boiling again, keeping the temperature at 90°C to 100°C for 1 hour, performing the second extraction, filtering with filter cloth after extraction to obtain filtrate b, adding water c to the filtered rose residue b, boiling again, keeping the temperature at 90°C to 100°C for 1 hour, performing the third extraction, filtering with filter cloth after extraction to obtain filtrate c, mixing the filtrate a, filtrate b and filtrate c, concentrating under reduced pressure to a clear paste with a relative density of 1.08 to 1.15, spray drying, crushing and sieving to obtain rose extract; S5, adding polyvinyl alcohol and sodium carboxymethyl cellulose into water, soaking and swelling, and heating at 80° C. to remove bubbles, to obtain a mixture A; S6, adding glycerin, the licorice chalcone extract obtained in S1, the reed root extract obtained in S2, the Cistanche deserticola extract obtained in S3 and the rose extract obtained in S4 to the mixture A obtained in S5, stirring and mixing evenly to obtain a mixture B; S7. Apply the uniform solution of the mixture B obtained in S6 onto the cleaned glass, and after drying, obtain an oral ulcer film containing multiple plant extracts.

3. The method according to claim 2, characterized in that The mass ratio of the dried licorice and water a in S1 is 1:10; the mass ratio of the filtered licorice residue and water b is 1:8; the mass ratio of the dried licorice residue and 60% ethanol solution is 1:

10.

4. The method according to claim 2, characterized in that: The mass ratio of the dried reed root and water a in S2 is 1:10; the mass ratio of the filtered reed root residue a and water b is 1:8; the mass ratio of the filtered reed root residue b and water c is 1:

8.

5. The method according to claim 2, characterized in that: In S3, the mass ratio of the dried Cistanche deserticola to the 60% ethanol solution a is 1:8; the mass ratio of the filtered Cistanche deserticola residue a to the 60% ethanol solution b is 1:8; the mass ratio of the filtered Cistanche deserticola residue b to the 60% ethanol solution c is 1:

8.

6. The method according to claim 2, characterized in that The mass ratio of the Hotan rose and water a described in S4 is 1:12; the mass ratio of the filtered rose residue a and water b is 1:10; the mass ratio of the filtered rose residue b and water c is 1:10.

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