Polypeptide synthesis equipment

Abstract

The embodiment of the invention provides polypeptide synthesis equipment. The polypeptide synthesis equipment comprises a reaction container, wherein a top opening is formed in the top of the reaction container, and a liquid outlet is formed in the bottom; the liquid discharging assembly comprises a positive pressure liquid discharging assembly and a negative pressure liquid discharging assembly, the positive pressure liquid discharging assembly is used for injecting gas into the reaction container from the top opening so as to apply pressure to the reaction container, and the negative pressure liquid discharging assembly is connected to the liquid discharging opening so as to pump out liquid in the reaction container through the liquid discharging opening. And the negative pressure liquid discharging assembly pumps out liquid in the reaction container through the liquid discharging opening, so that expected materials are left in the reaction container. The positive pressure drainage assembly can inject gas into the reaction container from the top opening so as to apply pressure to the interior of the reaction container. And the pressure can push the liquid in the reaction container, so that the liquid can be accelerated to be discharged through the liquid outlet. Therefore, the discharge speed of the liquid in the reaction container is relatively high, and the working efficiency of the polypeptide synthesis equipment is relatively high, so that the flux of polypeptide synthesis can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of polypeptide synthesis, and in particular to a polypeptide synthesis device. Background Art

[0002] As a key area of ​​innovative research and development in biomedicine, peptide synthesis technology has broad development prospects. Peptide drugs have become a hot spot in global new drug research and development due to their high biological activity, high specificity, low side effects, and low accumulation in the body. Currently, more than 80 peptide drugs have been approved for marketing worldwide, with a market size of US$62.8 billion in 2020 and expected to grow to US$96 billion in 2025, showing a strong growth momentum.

[0003] Conventional peptide synthesis equipment may include a reaction vessel and a drainage component. A resin may be placed in the reaction vessel. The resin may be used as a solid phase carrier, which is usually pre-activated and carries a protecting group such as Fmoc (fluorenylmethoxycarbonyl). First, a 20% piperidine solution may be added to the reaction vessel, and the piperidine solution may deprotect the resin, thereby removing the Fmoc protecting group at the N-terminus. Then, a gas such as nitrogen may be added to the reaction vessel, so that bubbling (condensation reaction) may be performed. The drainage component of the peptide synthesis equipment may generate a negative pressure, thereby extracting the liquid in the reaction vessel so that the desired material is left in the reaction vessel.

[0004] However, the efficiency of the liquid discharge component in extracting liquid is low, resulting in poor efficiency of peptide synthesis. In addition, it is difficult for the liquid discharge component to extract all the liquid, resulting in some liquid remaining in the reaction container, which in turn affects the next peptide synthesis. Summary of the invention

[0005] In order to at least partially solve the problems existing in the prior art, an embodiment of the present invention provides a polypeptide synthesis device. The polypeptide synthesis device includes: a reaction container, the top of the reaction container is provided with a top opening and the bottom is provided with a drainage port; and a drainage component, the drainage component includes a positive pressure drainage component and a negative pressure drainage component, the positive pressure drainage component is used to inject gas into the reaction container from the top opening to apply pressure to the reaction container, and the negative pressure drainage component is connected to the drainage port to extract the liquid in the reaction container through the drainage port.

[0006] Exemplarily, the polypeptide synthesis device further includes a drainage pipeline and a tube clamping assembly, wherein the drainage pipeline is connected between the negative pressure drainage assembly and the drainage port, and the tube clamping assembly is used to clamp the drainage pipeline to cut off the drainage pipeline.

[0007] Exemplarily, the tube clamping assembly includes a tube clamping body, a tube clamping drive and a pushing member. The tube clamping body is provided with a resistance surface. The driving end of the tube clamping drive is connected to the pushing member for driving the pushing member to move between a cut-off position and a conducting position. When the pushing member is in the cut-off position, it clamps the drainage pipeline with the resistance surface to cut off the drainage pipeline. When the pushing member is in the conducting position, it disengages from the drainage pipeline to conduct the drainage pipeline.

[0008] Exemplarily, a pair of limiting holes are also provided on the clamp body, and the limiting holes are located on both sides of the abutment surface along the extension direction of the drainage pipeline. The drainage pipeline passes through the pair of limiting holes in sequence, and the size of each of the pair of limiting holes is adapted to the drainage pipeline.

[0009] Exemplarily, the polypeptide synthesis equipment includes multiple groups of polypeptide synthesis devices, each of the multiple groups of polypeptide synthesis devices includes a drainage pipeline, a tube clamping assembly and multiple reaction containers, and in each group of the multiple groups of polypeptide synthesis devices, the drainage pipeline includes multiple shunt drainage pipelines corresponding to the multiple reaction containers one by one, the shunt drainage pipeline is connected between the negative pressure drainage assembly and its corresponding drainage port, and the tube clamping assembly is used to clamp the multiple shunt drainage pipelines in the group to which it belongs so that the multiple shunt drainage pipelines are cut off.

[0010] Exemplarily, each of the multiple groups of polypeptide synthesis devices also includes a confluence piece, and in each of the multiple groups of polypeptide synthesis devices, the drainage pipeline also includes a confluence drainage pipeline, and in each of the multiple groups of polypeptide synthesis devices, multiple diversion drainage pipelines, confluence pieces, confluence drainage pipelines and negative pressure drainage components are connected in sequence.

[0011] Exemplarily, the negative pressure liquid discharge component includes a negative pressure tank and a vacuum pump. The negative pressure tank is provided with a negative pressure port and a liquid inlet that are interconnected. The vacuum pump is connected to the negative pressure port for vacuuming the negative pressure tank, and the liquid inlet is connected to the liquid discharge port.

[0012] Exemplarily, a liquid level pipeline is also provided on the negative pressure tank, one end of the liquid level pipeline is connected to the bottom of the negative pressure tank, and the other end is connected to the top of the negative pressure tank, and a transparent window is provided on the liquid level pipeline, the bottom of the transparent window is not higher than the bottom of the negative pressure tank, and the top of the transparent window is not lower than the top of the negative pressure tank.

[0013] Exemplarily, the negative pressure tank is also provided with a positive pressure port connected to the negative pressure port and the liquid inlet and used to be connected to the gas source.

[0014] Exemplarily, the positive pressure drainage assembly includes a positive pressure pipeline, one end of which is open toward the top and the other end of which is used to be connected to an air source.

[0015] Exemplarily, the polypeptide synthesis equipment includes multiple groups of polypeptide synthesis devices, and the positive pressure pipeline includes a three-way joint and a connecting pipe. In each group of the multiple groups of polypeptide synthesis devices, the three-way joints are arranged one-to-one with the reaction vessels, and the first ends of the three-way joints are one-to-one toward each top opening in the group to which they are located. Along the arrangement direction of the three-way joints, the second end of the first three-way joint is used to connect to the gas source, and the third end of the last three-way joint is sealed with a plug, and the connecting pipe is connected between the second end and the third end of two adjacent three-way joints.

[0016] Exemplarily, the polypeptide synthesis device also includes an opening and closing cover assembly, which includes an opening and closing cover driving member and a cover plate, wherein the driving end of the opening and closing cover driving member is connected to the cover plate to drive the cover plate to move between an opening position and a closing position in a vertical direction, wherein the cover plate is spaced apart from the top opening when in the opening position, and is sealed to the top opening when in the closing position, and the positive pressure liquid discharge assembly is disposed on the cover plate, and is used to inject gas into the reaction vessel from the top opening when the cover plate is in the closing position.

[0017] Exemplarily, an elastic layer made of elastic material is provided on the cover plate, and when the cover plate is in the closed position, the elastic layer seals the top opening.

[0018] Exemplarily, a one-way valve connected to the drain port is disposed in the reaction container.

[0019] In practical applications of peptide synthesis equipment, after the reaction in the reaction vessel, the negative pressure drainage component can extract the liquid in the reaction vessel through the drainage port, so that the expected material is left in the reaction vessel. At the same time, the positive pressure drainage component can inject gas into the reaction vessel from the top opening to apply pressure to the reaction vessel. The pressure will push the liquid in the reaction vessel, so that the liquid can be discharged through the drainage port at an accelerated speed. In this way, the liquid in the reaction vessel is discharged faster, and the working efficiency of the peptide synthesis equipment is higher, thereby improving the flux of peptide synthesis. In addition, the pressure will also push the residual liquid on the inner wall of the reaction vessel to flow downward, so that the liquid is discharged through the drainage port. In this way, by setting a positive pressure drainage component, the residual liquid in the reaction vessel can be reduced to reduce the impact on subsequent processes.

[0020] A series of simplified concepts are introduced in the summary of the invention, which will be further described in detail in the detailed description. The summary of the invention does not mean to attempt to define the key features and essential technical features of the claimed technical solution, nor does it mean to attempt to determine the scope of protection of the claimed technical solution.

[0021] The advantages and features of the present invention are described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The following drawings of the present invention are used as part of the present invention for understanding the present invention. The drawings show the embodiments of the present invention and their descriptions, and are used to explain the principles of the present invention. In the drawings,

[0023] Figure 1 A perspective view of a polypeptide synthesis device according to an exemplary embodiment of the present invention;

[0024] Figure 2 for Figure 1 A top view of the first partial structure of the polypeptide synthesis device shown in FIG.

[0025] Figure 3 for Figure 1 A perspective view of the second part of the structure of the peptide synthesis device shown in FIG.

[0026] Figure 4 for Figure 3 A side view of the second part of the structure of the peptide synthesis device shown in FIG.

[0027] Figure 5 for Figure 4 A perspective view of the clamp assembly and the manifold shown in FIG.

[0028] Figure 6 for Figure 5 A front view of the clamp assembly and the manifold shown in FIG.

[0029] Figure 7 for Figure 5 A side view of the clamp assembly and manifold shown in FIG.

[0030] Figure 8 for Figure 2 A stereoscopic view of the negative pressure tank and components connected to the negative pressure tank is shown in FIG.

[0031] The above drawings include the following reference numerals:

[0032] 100, peptide synthesis device; 200, reaction container; 201, top opening; 202, liquid discharge port; 210, one-way valve; 220, reaction cup; 230, base; 300, positive pressure liquid discharge assembly; 310, positive pressure pipeline; 320, three-way connector; 330, air pressure detector; 400, negative pressure liquid discharge assembly; 410, negative pressure tank; 411, negative pressure port; 412, liquid inlet; 413, positive pressure port; 414, spare port; 420, vacuum pump; 430, liquid Body stop valve; 440, liquid level pipeline; 441, transparent window; 450, switch valve; 500, drainage pipeline; 510, shunt drainage pipeline; 600, tube clamping assembly; 610, tube clamping body; 611, limiting hole; 620, tube clamping drive member; 630, pushing member; 631, pushing member surface; 700, confluence member; 800, opening and closing cover assembly; 810, opening and closing cover drive member; 820, cover plate; 821, elastic layer; 830, transverse driving member; 910, cabinet body. DETAILED DESCRIPTION

[0033] In the following description, a large amount of details are provided so that the present invention can be thoroughly understood. However, it will be appreciated by those skilled in the art that the following description only exemplarily illustrates a preferred embodiment of the present invention, and the present invention can be implemented without one or more such details. In addition, in order to avoid confusion with the present invention, some technical features well known in the art are not described in detail.

[0034] The embodiment of the present invention provides a peptide synthesis device. The peptide synthesis device can be used to perform a peptide synthesis reaction, and thus can be used to synthesize peptides. The peptide synthesis device of the embodiment of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0035] like Figure 1-4 As shown, the polypeptide synthesis device may include a reaction vessel 200 and a drainage component.

[0036] The reaction vessel 200 can be used for carrying out a peptide synthesis reaction. The top of the reaction vessel 200 can be provided with a top opening 201. For example, materials such as resin, dimethylformamide (DMF) and / or piperidine solution can be added into the reaction vessel 200 through the top opening 201. The bottom of the reaction vessel 200 can be provided with a drain port 202. The liquid in the reaction vessel 200 can be discharged through the drain port 202. Exemplarily, the reaction vessel 200 can include a reaction cup 220 and a base 230. The base 230 can be installed below the reaction cup 220. The reaction cup 220 can only be seated on the base 230, or it can be connected to the base 230 by plugging, snapping or any other suitable method. The reaction cup 220 can be used for carrying out a peptide synthesis reaction. Exemplarily, the reaction cup 220 can be made of glass or other transparent materials. In this way, relevant personnel can conveniently observe the reaction situation inside the reaction cup 220 from the outside of the reaction cup 220. The top opening 201 can be set at the top of the reaction cup 220. The bottom of the reaction cup 220 may be provided with a bottom opening. The drain port 202 may be provided at the bottom of the base 230. A drain channel connected between the bottom opening of the reaction cup 220 and the drain port 202 may be provided in the base 230. In this way, the liquid may pass through the bottom opening and the drain channel of the reaction cup 220 in sequence, and thus may be discharged through the drain port 202. Exemplarily, a one-way valve 210 may also be provided in the reaction vessel 200. The one-way valve 210 may be connected to the drain port 202. In other words, the one-way valve 210 may allow the liquid to be unidirectionally conducted from the reaction cup 220 to the drain port 202. Specifically, the one-way valve 210 may be provided on the base 230. By providing the one-way valve 210, it is possible to prevent the liquid from flowing back into the reaction vessel 200 through the drain port 202. Exemplarily, the liquid includes but is not limited to DMF, dichloromethane (DCM) and / or piperidine solution, so the relevant components in the present application may have corrosion resistance and explosion-proof properties.

[0037] The discharge assembly may include a positive pressure discharge assembly 300 and a negative pressure discharge assembly 400. The positive pressure discharge assembly 300 may be used to inject gas (e.g., nitrogen) into the reaction vessel 200 from the top opening 201 to apply pressure to the reaction vessel 200. The positive pressure discharge assembly 300 includes, but is not limited to, an air pump assembly or a fan assembly, as long as gas can be injected into the reaction vessel 200 from the top opening 201 to apply pressure to the reaction vessel 200. The negative pressure discharge assembly 400 may be connected to the discharge port 202 to extract the liquid in the reaction vessel 200 through the discharge port 202. The negative pressure discharge assembly 400 includes, but is not limited to, a liquid pump assembly or a negative pressure tank assembly, as long as the liquid in the reaction vessel 200 can be extracted through the discharge port 202. Specifically, the negative pressure discharge assembly 400 may form a negative pressure. Under the effect of the negative pressure, the liquid in the reaction vessel 200 may be discharged through the discharge port 202.

[0038] In practical applications of the peptide synthesis device, after the reaction in the reaction container 200, the negative pressure drainage component 400 can extract the liquid in the reaction container 200 through the drainage port 202, so that the expected material is left in the reaction container 200. At the same time, the positive pressure drainage component 300 can inject gas into the reaction container 200 from the top opening 201 to apply pressure to the reaction container 200. The pressure will push the liquid in the reaction container 200, so that the liquid can be discharged through the drainage port 202 at an accelerated speed. In this way, the discharge speed of the liquid in the reaction container 200 is faster, and the working efficiency of the peptide synthesis device is higher, so that the flux of peptide synthesis can be improved. In addition, the pressure will also push the liquid remaining on the inner wall of the reaction container 200 to flow downward, so that the liquid is discharged through the drainage port 202. In this way, by setting the positive pressure drainage component 300, the residual liquid in the reaction container 200 can be reduced to reduce the impact on subsequent processes.

[0039] For example, Figure 3-7 As shown, the polypeptide synthesis device may further include a drainage pipeline 500 and a clamping tube assembly 600. The drainage pipeline 500 may be connected between the negative pressure drainage assembly 400 and the drainage port 202. The drainage pipeline 500 may adopt any suitable pipeline, for example, it may be a polymer pipeline. In this way, under the action of the negative pressure drainage assembly 400, the liquid in the reaction vessel 200 may be discharged to the negative pressure drainage assembly 400 through the drainage port 202 and the drainage pipeline 500 in sequence. The clamping tube assembly 600 may be used to clamp the drainage pipeline 500 so that the drainage pipeline 500 is cut off. Specifically, when the reaction is carried out in the reaction vessel 200, the clamping tube assembly 600 may clamp the drainage pipeline 500 so that the drainage pipeline 500 is cut off. In this way, the liquid in the reaction vessel 200 cannot be discharged through the drainage port 202. When the reaction is completed, the clamping tube assembly 600 may release the drainage pipeline 500 so that the drainage pipeline 500 is conductive. In this way, under the action of the negative pressure discharge assembly 400, the liquid in the reaction container 200 can be discharged to the negative pressure discharge assembly 400 through the discharge port 202 and the discharge pipeline 500 in turn. The clamp assembly 600 includes but is not limited to a clamp valve assembly. Compared with the structure of setting a stop valve on the discharge pipeline 500, the clamp assembly 600 does not need to contact the liquid in the discharge pipeline 500, so as not to cause pollution. In addition, the clamp assembly 600 does not cause the discharge pipeline 500 to increase the interface, so the leakage problem can be avoided.

[0040] For example, Figure 3-7As shown, the clamping tube assembly 600 may include a clamping tube body 610, a clamping tube driving member 620 and a pusher 630. A resisting surface (not shown due to angle problems) may be provided on the clamping tube body 610. The driving end of the clamping tube driving member 620 may be connected to the pusher 630 to drive the pusher 630 to move between the cut-off position and the conduction position. Wherein, when the pusher 630 is in the cut-off position, it can clamp the discharge pipeline 500 with the resisting surface of the clamping tube body 610 to cut off the discharge pipeline 500. When the pusher 630 is in the conduction position, it can be separated from the discharge pipeline 500 to conduct the discharge pipeline 500. The clamping tube driving member 620 includes but is not limited to a motor or a cylinder. Exemplarily, the pusher 630 may be located directly below the resisting surface of the clamping tube body 610. The clamping tube driving member 620 may drive the pusher 630 to move upward to the cut-off position, and drive the pusher 630 to move downward to the conduction position. Thus, when the pusher 630 moves downward to the conducting position, the discharge pipe 500 can restore its shape by itself under the action of gravity to be in the conducting state.

[0041] For example, Figure 5-6 As shown, a pair of limiting holes 611 may be further provided on the clamp body 610. Along the extension direction of the drainage pipeline 500, the pair of limiting holes 611 may be located on both sides of the abutment surface of the clamp body 610. The drainage pipeline 500 may pass through the pair of limiting holes 611 in sequence. The size of each of the pair of limiting holes 611 may be adapted to the drainage pipeline 500. The pair of limiting holes 611 may support and limit the drainage pipeline 500, so that the flexible drainage pipeline 500 may be in a desired position, so that the pusher 630 and the abutment surface of the clamp body 610 may clamp the drainage pipeline 500.

[0042] For example, Figure 5-6 As shown, the pusher 630 may have a pusher surface 631 facing the drain pipe 500. The pusher surface 631 may clamp the drain pipe 500 with the abutment surface of the clamp body 610. The pusher surface 631 may be an arcuate surface protruding toward the drain pipe 500. With such a configuration, the contact area between the arcuate surface and the drain pipe 500 is small, thereby increasing the clamping pressure and further increasing the cutoff effect on the drain pipe 500. In addition, the arcuate surface is not easy to damage the drain pipe 500.

[0043] Exemplarily, the surface roughness of the pusher surface 631 and the abutment surface of the clamping tube body 610 may be relatively low (eg, may be a mirror surface), thereby avoiding damage to the discharge pipeline 500 .

[0044] For example, Figure 5-6As shown, the clamping tube drive 620 may include a cylinder. The cylinder may be various types of cylinders known in the art or that may appear in the future, including but not limited to a three-axis cylinder. The piston rod of the cylinder may be connected to the pusher 630. Compared with the electric clamping tube drive, the cylinder generates less heat, thereby meeting the explosion-proof requirements.

[0045] For example, Figure 3-6 As shown, the polypeptide synthesis device may include multiple groups of polypeptide synthesis devices 100, such as two groups, three groups or more. In the embodiment shown in the figure, the polypeptide synthesis device may include six groups of polypeptide synthesis devices 100. Each group of the multiple groups of polypeptide synthesis devices 100 may include a drainage pipeline 500, a clamping tube assembly 600 and multiple reaction vessels 200. In the embodiment shown in the figure, each group of polypeptide synthesis devices 100 may include ten reaction vessels 200. The ten reaction vessels 200 may be arranged in a straight line. In each group of the multiple groups of polypeptide synthesis devices 100, the drainage pipeline 500 may include multiple shunt drainage pipelines 510. Multiple shunt drainage pipelines 510 may be arranged one by one with multiple reaction vessels 200. The shunt drainage pipeline 510 may be connected between the negative pressure drainage assembly 400 and its corresponding drainage port 202. In this way, under the action of the negative pressure drainage component 400, the liquid in the reaction container 200 can be discharged to the negative pressure drainage component 400 through its drainage port 202 and the shunt drainage pipeline 510 corresponding to the drainage port 202 in sequence. The clamping tube component 600 can be used to clamp multiple shunt drainage pipelines 510 in its group so that multiple shunt drainage pipelines 510 are cut off. In the embodiment where the limiting holes 611 are provided on the clamping tube body 610, each shunt drainage pipeline 510 can pass through a pair of limiting holes 611 in sequence. The size of each of the pair of limiting holes 611 can be adapted to the shunt drainage pipeline 510. With such a configuration, the polypeptide synthesis device can perform multiple groups of reactions at the same time, thereby improving the throughput of synthesized polypeptides. In addition, since the clamping tube component 600 can be used to clamp multiple shunt drainage pipelines 510 in its group, the liquid discharged through the drainage port 202 will not enter other reaction containers 200.

[0046] For example, Figure 5-7As shown, each group in the multiple groups of polypeptide synthesis devices 100 may also include a confluence piece 700. In each group in the multiple groups of polypeptide synthesis devices 100, the drainage pipeline 500 may also include a confluence drainage pipeline (not shown). In each group in the multiple groups of polypeptide synthesis devices 100, a plurality of shunt drainage pipelines 510, a confluence piece 700, a confluence drainage pipeline and a negative pressure drainage assembly 400 may be connected in sequence. That is to say, under the action of the negative pressure drainage assembly 400, the liquid in the reaction container 200 may be discharged to the negative pressure drainage assembly 400 through its drainage port 202, the shunt drainage pipeline 510 corresponding to the drainage port 202, the confluence piece 700 and the confluence drainage pipeline in sequence. By providing the confluence piece 700 and the confluence drainage pipeline, the number of pipelines can be reduced, thereby facilitating wiring. Furthermore, the number of interfaces (ie, the liquid inlet 412 described below) connected to the liquid discharge pipeline 500 can be reduced on the negative pressure liquid discharge assembly 400, thereby simplifying the structure.

[0047] For example, Figure 2 and 8 As shown, the negative pressure drainage assembly 400 may include a negative pressure tank 410 and a vacuum pump 420. The negative pressure tank 410 may be provided with a negative pressure port 411 and a liquid inlet 412. The negative pressure port 411 and the liquid inlet 412 may be interconnected. The vacuum pump 420 may be connected to the negative pressure port 411 to evacuate the negative pressure tank 410. The vacuum pump 420 may adopt various types of vacuum pumps known in the art or that may appear in the future, including but not limited to a diaphragm vacuum pump. The liquid inlet 412 may be connected to the drainage port 202. In an embodiment in which the polypeptide synthesis device includes a drainage pipeline 500, the drainage pipeline 500 may be connected between the drainage port 202 and the liquid inlet 412. Depending on the number of groups of the polypeptide synthesis device 100, the number of liquid inlets 412 includes but is not limited to one, two, three or more. Each liquid inlet 412 may be used to connect one or more drainage pipelines 500. The vacuum pump 420 can evacuate the negative pressure tank 410, thereby forming a negative pressure environment in the negative pressure tank 410. In this way, under the effect of negative pressure, the liquid in the reaction container 200 can be drawn out into the negative pressure tank 410 through the drain port 202. The negative pressure port 411 and the liquid inlet 412 can both be arranged at the top of the negative pressure tank 410. In this way, the liquid in the negative pressure tank 410 will not flow back out through the negative pressure port 411 and the liquid inlet 412.

[0048] like Figure 1-2 As shown, the negative pressure tank 410 and the vacuum pump 420 can be arranged in the cabinet 910 at the bottom of the peptide synthesis device. Multiple groups of peptide synthesis devices 100 can be arranged on the top of the cabinet 910. In this way, the liquid can also be discharged by gravity, thereby speeding up the discharge speed.

[0049] Since the liquid can be stored in the negative pressure tank 410, the negative pressure tank 410 can be made of corrosion-resistant materials. Exemplarily, the negative pressure tank 410 can be made of 304 stainless steel or 316 stainless steel. The negative pressure tank 410 can withstand at least one atmosphere of pressure. The volume of the negative pressure tank 410 can be around 36L. In the process of manufacturing the negative pressure tank 410, the inside and outside of the negative pressure tank 410 can be cleaned by a passivation cleaning solution.

[0050] For example, Figure 2 and 8 As shown, the negative pressure tank 410 may also be provided with a liquid outlet (not shown due to angle problems). The liquid outlet may be connected to the negative pressure port 411 and the liquid inlet 412. The liquid outlet may be used to connect to a liquid pipeline. The liquid in the negative pressure tank 410 may be discharged through the liquid outlet and the liquid pipeline in turn. The liquid outlet may usually be located at the bottom of the negative pressure tank 410 so that all the liquid can be discharged. A switch valve 450 may be provided on the liquid outlet to control the discharge of the liquid.

[0051] For example, Figure 2 and 8 As shown, a liquid level detector may also be provided on the negative pressure tank 410. A liquid stop valve 430 may be provided on the liquid pipeline. The polypeptide synthesis device may also include a controller. The controller may be electrically connected to the liquid level detector. The controller may be constructed using electronic components such as a timer, a comparator, a register, a digital logic circuit, or may be implemented using processor chips such as a single chip microcomputer, a microprocessor, a programmable logic controller (PLC), a digital signal processor (DSP), a field programmable gate array (FPGA), a programmable logic array (PLA), an application specific integrated circuit (ASIC), and their peripheral circuits. The liquid level detector may be used to detect the liquid level in the negative pressure tank 410. When the liquid level in the negative pressure tank 410 reaches the expected liquid level, the liquid level detector may send a signal to the controller. The controller may be used to control the liquid stop valve 430 to be turned on based on the signal, and to control the vacuum pump 420 to stop. After the vacuum pump 420 is stopped, the liquid will not continue to enter the negative pressure tank 410. After the liquid stop valve 430 is turned on, the liquid may be discharged through the liquid pipeline. In this way, the liquid in the negative pressure tank 410 can be automatically discharged to avoid excessive liquid in the negative pressure tank 410, which may lead to dangerous accidents. When the discharge is completed (for example, when the liquid level detector detects that the liquid level in the negative pressure tank 410 reaches the lowest level), the controller can control the liquid stop valve 430 to be closed and control the vacuum pump 420 to start, so that the liquid can continue to enter the negative pressure tank 410.

[0052] For example, Figure 2 and 8As shown, a liquid level pipeline 440 may be further provided on the negative pressure tank 410. One end of the liquid level pipeline 440 may be connected to the bottom of the negative pressure tank 410. The other end of the liquid level pipeline 440 may be connected to the top of the negative pressure tank 410. A transparent window 441 may be provided on the liquid level pipeline 440. Exemplarily, the transparent window 441 may be a transparent pipe section made of transparent Teflon or an equivalent corrosion-resistant material. The bottom of the transparent window 441 is not higher than the bottom of the negative pressure tank 410. The top of the transparent window 441 is not lower than the top of the negative pressure tank 410. In this way, relevant personnel can observe the liquid level of the negative pressure tank 410 through the transparent window 441.

[0053] For example, Figure 2 and 8 As shown, a positive pressure port 413 may also be provided on the negative pressure tank 410. The positive pressure port 413 may be connected to the negative pressure port 411 and the liquid inlet 412. The positive pressure port 413 may be used to connect to an air source. In this arrangement, when the negative pressure tank 410 needs to discharge liquid through the liquid outlet, the air source may inject gas (e.g., nitrogen) into the negative pressure tank 410 through the positive pressure port 413, thereby accelerating the discharge of the negative pressure tank 410. Exemplarily, the positive pressure port 413 may be provided at the top of the negative pressure tank 410. In this way, the liquid in the negative pressure tank 410 will not flow back out through the positive pressure port 413.

[0054] For example, Figure 2 and 8 As shown, the negative pressure tank 410 may also be provided with a spare port 414. The spare port 414 may be blocked with a plug.

[0055] For example, Figure 3-4 As shown, the positive pressure drainage assembly 300 may include a positive pressure line 310. One end of the positive pressure line 310 may face the top opening 201. The other end of the positive pressure line 310 may be used to connect to a gas source. The gas source and the gas source connected to the positive pressure port 413 may be the same or different gas sources. The gas source may supply gas so that the gas may pass through the positive pressure line 310 and be injected into the reaction vessel 200.

[0056] For example, Figure 3-4 As shown, each of the multiple groups of polypeptide synthesis devices 100 may include a positive pressure drainage assembly 300 and multiple reaction containers 200. One end of the positive pressure pipeline 310 may be directed toward each top opening 201 in the group in which it is located.

[0057] For example, Figure 3-4As shown, the positive pressure pipeline 310 may include a three-way joint 320 and a connecting pipe (not shown). In each group of the multiple groups of polypeptide synthesis devices 100, the three-way joint 320 can be arranged one-to-one with the reaction vessel 200. The first end of the three-way joint 320 can be one-to-one toward each top opening 201 in the group where it is located. Along the arrangement direction of the three-way joints 320, the second end of the first three-way joint 320 can be used to connect to the gas source. The third end of the last three-way joint 320 can be blocked with a plug to prevent gas from flowing out. The connecting pipe can be connected between the second end and the third end of two adjacent three-way joints 320. In this way, the gas source can supply gas so that the gas can flow from the first three-way joint 320 to the last three-way joint 320 along the arrangement direction of the three-way joints 320, and the gas is injected into the reaction vessel 200 through the first end of each three-way joint 320. In this way, the structure of the positive pressure pipeline 310 is relatively simple.

[0058] For example, Figure 3-4 As shown, a gas pressure detector 330 may be connected to the positive pressure pipeline 310. The gas pressure detector 330 includes but is not limited to a barometer or a gas pressure sensor. The gas pressure detector 330 may be used to detect the pressure of the gas.

[0059] For example, Figure 3-4 As shown, the polypeptide synthesis device may further include an opening and closing cover assembly 800. The opening and closing cover assembly 800 may include an opening and closing cover drive 810 and a cover plate 820. The driving end of the opening and closing cover drive 810 may be connected to the cover plate 820 to drive the cover plate 820 to move between the opening position and the closing position in the vertical direction. When the cover plate 820 is in the opening position, it may be spaced apart from the top opening 201. In this manner, materials may be added to the reaction vessel 200 through the top opening 201. When the cover plate 820 is in the closing position, it may be sealed to the top opening 201. The opening and closing cover drive 810 includes but is not limited to a motor or a cylinder. The positive pressure discharge assembly 300 may be disposed on the cover plate 820. Specifically, the three-way connector 320 may be disposed on the cover plate 820. When the cover plate 820 is in the closing position, the positive pressure discharge assembly 300 may inject gas from the top opening 201 into the reaction vessel 200. By sealing the top opening 201 with the cover plate 820 , the pressure in the reaction container 200 can be increased rapidly, thereby forming a positive pressure environment and further improving the effect of liquid discharge.

[0060] For example, Figure 3-4As shown, the opening and closing cover assembly 800 may also include a transverse drive 830. The driving end of the transverse drive 830 may be connected to the main body of the opening and closing cover drive 810. In this way, the transverse drive 830 may drive the opening and closing cover drive 810 and the cover plate 820 to move synchronously. When the cover plate 820 is in the opening position, the transverse drive 830 may be used to drive the opening and closing cover drive 810 and the cover plate 820 to move between the staggered position and the aligned position in the horizontal direction. When the cover plate 820 is in the staggered position, it may be staggered with the top opening 201 in the horizontal direction. With such an arrangement, it is more convenient to add materials into the reaction vessel 200 through the top opening 201. When the cover plate 820 is in the aligned position, it may be directly above the top opening 201. In this way, the opening and closing cover drive 810 may drive the cover plate 820 to move between the opening position and the closing position in the vertical direction. The transverse drive 830 includes but is not limited to a motor or a cylinder.

[0061] For example, Figure 3-4 As shown, an elastic layer 821 made of an elastic material (such as rubber or silicone) may be provided on the cover plate 820. When the cover plate 820 is in the closed position, the elastic layer 821 may seal the top opening 201. By providing the elastic layer 821, the sealing performance between the cover plate 820 and the reaction container 200 may be improved, which may further facilitate the formation of a positive pressure environment, thereby improving the effect of liquid discharge.

[0062] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by directional words such as "front", "back", "up", "down", "left", "right", "lateral", "vertical", "vertical", "horizontal", "top", "bottom", etc. is usually based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the scope of protection of the present invention; the directional words "inside" and "outside" refer to the inside and outside relative to the contour of each component itself.

[0063] For ease of description, regional relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the regional positional relationship between one or more components or features shown in the figure and other components or features. It should be understood that regional relative terms include not only the orientation of the components as described in the figure, but also different orientations in use or operation. For example, if the components in the accompanying drawings are inverted as a whole, the components "above other components or features" or "above other components or features" will include the situation where the components are "below other components or structures" or "below other components or structures". Therefore, the exemplary term "above" may include both "above" and "below". In addition, these components or features may also be positioned at other different angles (e.g., rotated 90 degrees or other angles), and this article is intended to include all of these situations.

[0064] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, parts, components and / or combinations thereof.

[0065] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the numbers used in this way can be interchanged where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein.

[0066] The present invention has been described by the above embodiments, but it should be understood that the above embodiments are only for the purpose of example and description, and are not intended to limit the present invention to the scope of the described embodiments. In addition, it can be understood by those skilled in the art that the present invention is not limited to the above embodiments, and more variations and modifications can be made according to the teachings of the present invention, and these variations and modifications all fall within the scope of the protection claimed by the present invention. The protection scope of the present invention is defined by the attached claims and their equivalents.

Claims

1. A polypeptide synthesis device, characterized in that: include: A reaction container, wherein the top of the reaction container is provided with a top opening and the bottom of the reaction container is provided with a liquid discharge port; as well as The drainage component includes a positive pressure drainage component and a negative pressure drainage component. The positive pressure drainage component is used to inject gas into the reaction container from the top opening to apply pressure to the reaction container. The negative pressure drainage component is connected to the drainage port to extract the liquid in the reaction container through the drainage port.

2. The peptide synthesis device according to claim 1, characterized in that: The polypeptide synthesis equipment also includes a drainage pipeline and a pipe clamping assembly. The drainage pipeline is connected between the negative pressure drainage assembly and the drainage port. The pipe clamping assembly is used to clamp the drainage pipeline to cut off the drainage pipeline.

3. The polypeptide synthesis device according to claim 2, characterized in that: The tube clamping assembly includes a tube clamping body, a tube clamping driving member and a pushing member. The tube clamping body is provided with a resistance surface. The driving end of the tube clamping driving member is connected to the pushing member to drive the pushing member to move between a cut-off position and a conducting position. When the pushing member is in the cut-off position, it clamps the drainage pipeline with the resistance surface to cut off the drainage pipeline. When the pushing member is in the conducting position, it disengages from the drainage pipeline to conduct the drainage pipeline.

4. The polypeptide synthesis device according to claim 3, characterized in that: A pair of limiting holes is also provided on the clamp body, and the pair of limiting holes are located on both sides of the abutment surface along the extension direction of the drainage pipeline. The drainage pipeline passes through the pair of limiting holes in sequence, and the size of each of the pair of limiting holes is adapted to the drainage pipeline.

5. The peptide synthesis device according to claim 2, characterized in that: The polypeptide synthesis equipment comprises a plurality of groups of polypeptide synthesis devices, each of which comprises a drainage pipeline, a tube clamping assembly and a plurality of reaction containers. In each of the plurality of groups of polypeptide synthesis devices, the drainage pipeline comprises a plurality of shunt drainage pipelines corresponding one by one to the plurality of reaction containers, the shunt drainage pipelines are connected between the negative pressure drainage assembly and the corresponding drainage port, and the tube clamping assembly is used to clamp the plurality of shunt drainage pipelines in the group to which it belongs so as to cut off the plurality of shunt drainage pipelines.

6. The peptide synthesis device according to claim 5, characterized in that: Each of the multiple groups of polypeptide synthesis devices further includes a confluence piece, and in each of the multiple groups of polypeptide synthesis devices, the drainage pipeline further includes a confluence drainage pipeline, and in each of the multiple groups of polypeptide synthesis devices, the multiple diversion drainage pipelines, the confluence piece, the confluence drainage pipeline and the negative pressure drainage component are connected in sequence.

7. The peptide synthesis device according to claim 1, characterized in that: The negative pressure liquid discharge component includes a negative pressure tank and a vacuum pump. The negative pressure tank is provided with a negative pressure port and a liquid inlet which are interconnected. The vacuum pump is connected to the negative pressure port to evacuate the negative pressure tank. The liquid inlet is connected to the liquid discharge port.

8. The peptide synthesis device according to claim 7, characterized in that: The negative pressure tank is also provided with a liquid level pipeline, one end of which is connected to the bottom of the negative pressure tank, and the other end is connected to the top of the negative pressure tank. A transparent window is provided on the liquid level pipeline, the bottom of which is not higher than the bottom of the negative pressure tank, and the top of which is not lower than the top of the negative pressure tank.

9. The polypeptide synthesis device according to claim 7, characterized in that: The negative pressure tank is also provided with a positive pressure port connected to the negative pressure port and the liquid inlet and used for connecting to an air source.

10. The peptide synthesis device according to claim 1, characterized in that: The positive pressure liquid discharge assembly comprises a positive pressure pipeline, one end of which is open toward the top, and the other end of which is used to be connected to an air source.

11. The peptide synthesis device according to claim 10, characterized in that: The polypeptide synthesis equipment comprises a plurality of groups of polypeptide synthesis devices, the positive pressure pipeline comprises a three-way joint and a connecting pipe, in each group of the plurality of groups of polypeptide synthesis devices, the three-way joint is arranged one-to-one with the reaction container, the first end of the three-way joint is one-to-one toward each of the top openings in the group where it is located, along the arrangement direction of the three-way joints, the second end of the first three-way joint is used to be connected to the gas source, the third end of the last three-way joint is blocked with a plug, and the connecting pipe is connected between the second end and the third end of two adjacent three-way joints.

12. The peptide synthesis device according to claim 1, characterized in that: The polypeptide synthesis device also includes an opening and closing cover assembly, which includes an opening and closing cover driving member and a cover plate. The driving end of the opening and closing cover driving member is connected to the cover plate to drive the cover plate to move between an opening position and a closing position in a vertical direction. When the cover plate is in the opening position, it is spaced from the top opening. When the cover plate is in the closing position, it is blocked to the top opening. The positive pressure liquid discharge assembly is arranged on the cover plate and is used to inject gas into the reaction container from the top opening when the cover plate is in the closing position.

13. The peptide synthesis device according to claim 12, characterized in that: An elastic layer made of elastic material is arranged on the cover plate, and when the cover plate is in the closing position, the elastic layer seals the top opening.

14. The peptide synthesis device according to claim 1, characterized in that: A one-way valve connected to the liquid discharge port is arranged in the reaction container.

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