Reservoir floccule degradation method based on microbial technology

Abstract

The invention provides a reservoir floccule degradation method based on a microbial technology. The reservoir floccule degradation method based on the microbial technology comprises the following steps: a, carrying out comprehensive water quality analysis on a reservoir water body, including determination of organic matter concentration, floccule quantity, pH value, dissolved oxygen content and temperature parameters, so as to determine causes and distribution characteristics of the reservoir floccules, and analyzing an existing microbial community structure in the water body by adopting a high-throughput sequencing technology; and identifying the types and functions of the key microorganisms so as to optimize the formula of the subsequent microbial agent. According to the reservoir floccule degradation method based on the microbial technology, the problem that the degradation efficiency is unstable due to the fact that the microbial activity is limited by environmental conditions is effectively solved by accurately regulating and controlling the feeding amount of a microbial agent and the bacterial strain proportion. Specifically, through comprehensive water quality analysis and microbial community structure evaluation, the formula of the microbial agent can be optimized, so that the microbial agent can better adapt to the actual environment of the reservoir.

Description

Technical Field

[0001] The invention relates to the technical field of reservoir floc degradation, in particular to a reservoir floc degradation method based on microbial technology. Background Art

[0002] The methods for degrading reservoir flocs based on microbial technology mainly include aerobic microbial degradation, anaerobic microbial degradation, application of composite microbial agents, and biofilm technology. These methods improve water quality by selecting specific biosafety microbial strains, such as aerobic or anaerobic bacteria such as Pseudomonas and Bacillus, to decompose organic matter and flocs in water bodies under suitable environmental conditions. The use of aerobic composite agents significantly reduces the concentration of organic matter and improves water transparency; in urban drinking water source protection areas, the anaerobic microbial degradation method reduces the organic matter content in the bottom mud and extends the service life of the water source protection area. In addition, biofilm technology continuously degrades organic pollutants in runoff by fixing the microbial community on the carrier, thereby promoting the recovery of the shore ecosystem.

[0003] However, the activity of microorganisms is restricted by environmental conditions and fluctuates, which affects the degradation efficiency of microorganisms and leads to unstable treatment effects. Although biosafe microbial agents are selected, long-term and large-scale addition may change the structure of microbial communities in the water body, thereby affecting the ecological balance of the reservoir. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides a reservoir floc degradation method based on microbial technology, which solves the problem that the activity of microorganisms is restricted by environmental conditions and fluctuates, affecting the degradation efficiency of the microorganisms and resulting in unstable treatment effect; and the problem that long-term and large-scale addition may change the structure of the microbial community in the water body and thus affect the ecological balance of the reservoir.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: A reservoir floc degradation method based on microbial technology comprises the following steps:

[0006] a. Conduct a comprehensive water quality analysis of the reservoir water, including the determination of organic matter concentration, floc quantity, pH value, dissolved oxygen content, and temperature parameters, to determine the cause and distribution characteristics of floc in the reservoir, and use high-throughput sequencing technology to analyze the existing microbial community structure in the water body, identify key microbial species and their functions, so as to optimize the formulation of subsequent microbial agents;

[0007] b. Cultivate aerobic bacteria, anaerobic bacteria and auxiliary microbial strains under sterile conditions, and amplify each strain through fermentation technology to ensure its activity and degradation ability, and mix each component according to the following mass percentage:

[0008] Aerobic strains, accounting for 40% of the total amount of the inoculum, including 20% ​​of Pseudomonas and 20% of Bacillus;

[0009] Anaerobic bacteria, accounting for 30% of the total amount of the bacterial agent, including 15% of methanogens and 15% of sulfate-reducing bacteria;

[0010] Auxiliary microorganisms, accounting for 15% of the total amount of the inoculant, including 10% of fungi and 5% of enzyme-producing bacteria;

[0011] Nutritional additives, accounting for 10% of the total amount of the inoculant, including 4% amino acid mixture, 3% potassium dihydrogen phosphate, and 3% iron, zinc, and magnesium in total;

[0012] Protective agent, accounting for 5% of the total amount of the microbial agent, including 3% glycerol and 2% sucrose;

[0013] Carrier materials, accounting for 10% of the total amount of the microbial agent, including 5% bentonite and 5% activated carbon;

[0014] The above ingredients are fully mixed under sterile conditions to evenly distribute the microorganisms and nutrients on the carrier material, and freeze-dried to extend the shelf life of the bacterial agent and facilitate transportation and storage;

[0015] c. Determine the dosage of the microbial agent based on the water organic matter concentration, flocculent quantity and water volume obtained in the pretreatment and assessment steps, and evenly distribute the prepared microbial agent in the reservoir water to ensure uniform distribution and effective contact of the microorganisms in the water;

[0016] d. Install temperature sensors, pH sensors and dissolved oxygen sensors in the reservoir to monitor water environment parameters in real time through an automated control system;

[0017] e. Collect water samples regularly, use high-throughput sequencing technology and water quality analysis methods to monitor the microbial community structure, floc residue and organic matter degradation rate, and adjust the dosage and strain ratio of microbial agents in a timely manner according to the monitoring results to avoid significant changes in the microbial community structure and maintain the ecological balance of the reservoir;

[0018] f. In the process of floc degradation, intermediate products or by-products are generated, which are monitored through water sampling and analysis, and the by-products produced by degradation are treated by physical filtration, chemical neutralization or further biodegradation methods to prevent secondary pollution;

[0019] g. After the degradation process is completed, conduct a comprehensive water quality assessment of the water body to confirm the flocculent degradation effect and the degree of water quality improvement, prepare a detailed project evaluation report, and record the changes in various parameters, degradation efficiency and ecological impact during the degradation process as a basis for subsequent optimization and reference;

[0020] h. Through automated control systems and regular monitoring, abnormal environmental parameters or unexpected problems during the degradation process can be discovered in a timely manner. According to the preset emergency plan, rapid adjustments to the dosage, suspension of dosage or other remedial measures can be taken to ensure the safety and stability of the degradation process.

[0021] Preferably, the auxiliary microorganisms include fungi and enzyme-producing bacteria, which are used to enhance the degradation efficiency of flocs by decomposing complex organic polymers and secreting a variety of enzymes. The protective agent is selected from glycerol and sucrose, which is used to improve the survival rate and adaptability of microbial agents in water bodies.

[0022] Preferably, the dosage of the microbial agent is adjusted according to the total volume of the reservoir water and the amount of flocs. The dosage of the microbial agent is 1 kg to 5 kg per cubic meter of water, and is added in stages and batches through an automated dosing device to ensure uniform distribution of microorganisms and not cause ecological imbalance in the water body.

[0023] Preferably, the microbial agent is stored in sealed packaging to prevent the external environment from affecting the activity of the agent, and the sealed packaging material is food-grade moisture-proof and anti-oxidation material.

[0024] Preferably, during the addition of the microbial agent, a plurality of microbial distribution monitoring points are set in the reservoir, and data is transmitted to a control center in real time through a wireless sensor network, thereby achieving accurate addition of microorganisms and position optimization.

[0025] Preferably, the enzyme-producing bacteria in the microbial agent include β-glucosidase, lipase and protease, wherein β-glucosidase is used to decompose cellulose substances, lipase decomposes fat pollutants, and protease is used to degrade protein organic matter in water.

[0026] Preferably, the water temperature is between 20°C and 30°C, the pH value is between 6.5 and 8.5, and the dissolved oxygen concentration is above 5 mg / L to maintain the optimal activity of the microorganisms.

[0027] Preferably, the water quality analysis further analyzes heavy metal pollutants in the reservoir water, detects lead, mercury, and cadmium heavy metals in the water by high performance liquid chromatography or atomic absorption spectrometry, and adjusts the ratio of microbial agents according to the test results to increase the degradation or adsorption capacity of these heavy metals.

[0028] The present invention provides a reservoir floc degradation method based on microbial technology, which has the following beneficial effects:

[0029] This reservoir floc degradation method based on microbial technology effectively solves the problem of unstable degradation efficiency caused by the limitation of environmental conditions on microbial activity by precisely controlling the dosage and strain ratio of microbial agents. Specifically, through comprehensive water quality analysis and evaluation of microbial community structure, the formulation of microbial agents can be optimized to better adapt to the actual environment of the reservoir. This method can precisely control the distribution of microorganisms and avoid the changes in the microbial community structure of the water body caused by long-term and large-scale addition of agents, thereby effectively avoiding the problem of imbalance in the reservoir ecosystem.

[0030] The effectiveness and stability of microbial agents in water bodies are ensured by adopting advanced microbial culture, freeze-drying preservation technology, and automated control systems. During the addition process, the optimal activity of microorganisms can be maintained by real-time monitoring of water quality changes in the reservoir, especially key environmental parameters such as dissolved oxygen, pH value, and temperature. In addition, the use of sealed packaging of microbial agents can prevent the external environment from affecting their activity, ensuring the long-term storage of the agents and their stability during transportation, thereby improving the effectiveness and reliability of the degradation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a flow chart of the present invention. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Embodiment 1:

[0034] like Figure 1 As shown, the embodiment of the present invention provides a reservoir floc degradation method based on microbial technology, including: a. conducting a comprehensive water quality analysis of the reservoir water body, including determination of organic matter concentration, floc quantity, pH value, dissolved oxygen content, and temperature parameters to determine the cause of reservoir floc and its distribution characteristics, using high-throughput sequencing technology to analyze the existing microbial community structure in the water body, identifying key microbial species and their functions, so as to optimize the formulation of subsequent microbial agents;

[0035] b. Cultivate aerobic bacteria, anaerobic bacteria and auxiliary microbial strains under sterile conditions, and amplify each strain through fermentation technology to ensure its activity and degradation ability, and mix each component according to the following mass percentage:

[0036] Aerobic strains, accounting for 40% of the total amount of the inoculum, including 20% ​​of Pseudomonas and 20% of Bacillus;

[0037] Anaerobic bacteria, accounting for 30% of the total amount of the bacterial agent, including 15% of methanogens and 15% of sulfate-reducing bacteria;

[0038] Auxiliary microorganisms, accounting for 15% of the total amount of the microbial agent, including 10% of fungi and 5% of enzyme-producing bacteria, the auxiliary microorganisms are used to enhance the degradation efficiency of floccules by decomposing complex organic polymers and secreting a variety of enzymes, and the protective agent is selected from glycerol and sucrose, and is used to improve the survival rate and adaptability of the microbial agent in the water body;

[0039] Nutritional additives, accounting for 10% of the total amount of the inoculant, including 4% amino acid mixture, 3% potassium dihydrogen phosphate, and 3% iron, zinc, and magnesium in total;

[0040] Protective agent, accounting for 5% of the total amount of the microbial agent, including 3% glycerol and 2% sucrose;

[0041] Carrier materials, accounting for 10% of the total amount of the microbial agent, including 5% bentonite and 5% activated carbon;

[0042] The above ingredients are fully mixed under sterile conditions to evenly distribute the microorganisms and nutrients on the carrier material, and freeze-dried to extend the shelf life of the bacterial agent and facilitate transportation and storage;

[0043] c. According to the water organic matter concentration, floc number and water volume obtained in the pretreatment and evaluation steps, determine the dosage of the microbial agent, evenly distribute the prepared microbial agent in the reservoir water to ensure the uniform distribution and effective contact of the microorganisms in the water. The dosage of the microbial agent is adjusted according to the total volume of the reservoir water and the number of flocs. The dosage of the microbial agent is 1 kg to 5 kg per cubic meter of water, and is added in stages and batches through an automated dosing device to ensure uniform distribution of microorganisms and not cause ecological imbalance in the water. The storage method of the microbial agent is sealed packaging to prevent the external environment from affecting the agent. activity, the sealing packaging material is food-grade moisture-proof and anti-oxidation material, and during the addition of the microbial agent, multiple microbial distribution monitoring points are set in the reservoir, and data is transmitted to the control center in real time through a wireless sensor network, so as to achieve accurate addition of microorganisms and position optimization. The enzyme-producing bacteria in the microbial agent include β-glucosidase, lipase and protease, among which β-glucosidase is used to decompose cellulose substances, lipase decomposes fat pollutants, and protease is used to degrade protein organic matter in the water body. During the addition process, a layered addition strategy is adopted in combination with different areas and different depths of the reservoir water body, such as the water surface and bottom water. In shallow water areas, the dosage can be increased appropriately to ensure efficient degradation of flocs in shallow water areas; in deep water areas, the optimal dosage is calculated through diffusion and flow models to ensure uniform distribution of microorganisms and degradation efficiency;

[0044] d. Install temperature sensors, pH sensors and dissolved oxygen sensors in the reservoir to monitor water environment parameters in real time. Through the automatic control system, the water temperature is between 20°C and 30°C, the pH value is between 6.5 and 8.5, and the dissolved oxygen concentration is above 5 mg / L to maintain the optimal activity of microorganisms;

[0045] e. Collect water samples regularly, use high-throughput sequencing technology and water quality analysis methods to monitor the microbial community structure, floc residue and organic matter degradation rate, and adjust the dosage and strain ratio of microbial agents in a timely manner according to the monitoring results to avoid significant changes in the microbial community structure and maintain the ecological balance of the reservoir. The water quality analysis further analyzes the heavy metal pollutants in the reservoir water, detects the lead, mercury and cadmium heavy metals in the water by high-performance liquid chromatography or atomic absorption spectrometry, and adjusts the proportion of microbial agents according to the test results to increase the degradation or adsorption capacity of these heavy metals;

[0046] f. In the process of floc degradation, intermediate products or by-products are generated, which are monitored through water sampling and analysis, and the by-products produced by degradation are treated by physical filtration, chemical neutralization or further biodegradation methods to prevent secondary pollution;

[0047] g. After the degradation process is completed, a comprehensive water quality assessment is conducted on the water body to confirm the floc degradation effect and the degree of water quality improvement. A detailed project assessment report is prepared to record the changes in various parameters, degradation efficiency and ecological impact during the degradation process as a basis for subsequent optimization and reference. By introducing microclimate control technologies such as regular aeration, temperature and humidity control, and ecological restoration measures such as plant planting and introduction of aquatic animals, the overall balance of the reservoir ecosystem is further adjusted to ensure that the long-term application of microbial agents will not cause ecological degradation or imbalance, and maintain the diversity and self-purification capacity of the water body. In the process of water quality assessment, in addition to the evaluation of the floc degradation effect, a comprehensive evaluation of other ecological indicators in the reservoir should be conducted, such as dissolved oxygen, nitrogen-phosphorus ratio, and plankton number ecological health indicators of the water body, to ensure that the use of microbial agents will not lead to the simplification of the reservoir ecosystem. The evaluation results are used to regularly adjust the use strategy of microbial agents and optimize the water quality of the reservoir in combination with ecological restoration measures;

[0048] h. Through automated control systems and regular monitoring, abnormal environmental parameters or unexpected problems during the degradation process can be discovered in a timely manner. According to the preset emergency plan, rapid adjustments to the dosage, suspension of dosage or other remedial measures can be taken to ensure the safety and stability of the degradation process.

[0049] Example 2: This example selects a typical reservoir to conduct a microbial agent placement and floc degradation experiment. The reservoir water contains relatively high concentrations of organic matter and flocs. After analysis, it is found that the main pollutants in the water are organic matter (for example, plant debris, animal carcasses, etc.) and flocs produced by the decomposition of these organic matter.

[0050] Reservoir water quality analysis:

[0051] Organic matter concentration: about 150mg / L.

[0052] Floc quantity: about 3,000mg / L.

[0053] pH: 7.4.

[0054] Dissolved oxygen: 6.2mg / L.

[0055] Temperature: 26℃.

[0056] Microbial community: Through high-throughput sequencing technology analysis, it was found that Pseudomonas, Bacillus, sulfate-reducing bacteria, and methanogens were the main bacteria in the water, but the overall number was low.

[0057] Microbial inoculant formula:

[0058] According to the above water quality analysis, the composition of the microbial agent is optimized and the following ratio is selected:

[0059] Aerobic strains: 40% of the total amount of bacteria

[0060] Pseudomonas: 20%

[0061] Bacillus: 20%

[0062] Anaerobic strains: 30% of the total amount of bacteria

[0063] Methanogens: 15%

[0064] Sulfate-reducing bacteria: 15%

[0065] Auxiliary microorganisms: 15% of the total amount of microbial preparations

[0066] Fungi: 10%

[0067] Enzyme-producing bacteria: 5%

[0068] Nutritional additives: 10% of the total amount of microbial agents

[0069] Amino Acid Blend: 4%

[0070] Potassium dihydrogen phosphate: 3%

[0071] Iron, zinc, magnesium: 3% in total

[0072] Protective agent: 5% of the total amount of microbial agent

[0073] Glycerin: 3%

[0074] Sucrose: 2%

[0075] Carrier material: 10% of the total amount of the microbial agent

[0076] Bentonite: 5%

[0077] Activated carbon: 5%

[0078] Preparation of microbial inoculants:

[0079] Aerobic bacteria, anaerobic bacteria and auxiliary microbial strains are cultured under sterile conditions for fermentation amplification.

[0080] The ingredients are mixed according to the above ratio and freeze-dried to ensure the activity and stability of the microorganisms.

[0081] Microbial agents are stored in sealed packages using food-grade moisture-proof and anti-oxidation materials.

[0082] Delivery plan:

[0083] Determination of the quantity to be released:

[0084] The amount of bacterial agent to be added is determined based on the water volume and floc count of the reservoir. The total volume of the reservoir water is 500,000 cubic meters, and the floc count is 3,000 mg / L.

[0085] The recommended dosage of microbial agents is 1-5 kg ​​per cubic meter of water. Therefore, the recommended dosage is:

[0086] Minimum delivery volume: 500,000m 3 ×1kg / m 3 =500,000kg

[0087] Maximum delivery volume: 500,000m 3 ×5kg / m 3 =2,500,000kg

[0088] The median amount released is: 1,500,000kg.

[0089] Delivery method:

[0090] Through the automated dosing system, the microbial agent is added in stages and batches. In the initial stage, 1,000 kg of microbial agent can be added, and then gradually increased to 1,500,000 kg to ensure the uniform distribution and degradation effect of the microbial agent in the reservoir water.

[0091] Microbial monitoring and regulation:

[0092] Multiple microbial distribution monitoring points are set up in the reservoir, and data are collected in real time through a wireless sensor network.

[0093] According to real-time data, adjust the dosage of microbial agents to ensure uniform distribution and degradation effect of microorganisms, while avoiding ecological imbalance of water bodies caused by excessive dosage.

[0094] Monitoring and data feedback:

[0095] Temperature sensors, pH sensors and dissolved oxygen sensors are installed in the reservoir to monitor water environment changes in real time.

[0096] An automated control system is used to adjust the aeration equipment, pH adjustment equipment and dosing equipment according to environmental data to maintain the water temperature between 20°C and 30°C, the pH value between 6.5 and 8.5, and the dissolved oxygen concentration above 5 mg / L to ensure optimal activity of microorganisms.

[0097] Water quality monitoring and adjustment:

[0098] Collect water samples regularly, analyze water quality changes, and monitor the progress of floc degradation.

[0099] High-throughput sequencing technology is used to analyze the microbial community structure in water bodies to ensure the stability of the microbial community and avoid over-reliance on a single species of bacteria that may lead to ecological imbalance.

[0100] According to water quality monitoring data, timely adjust the dosage ratio and amount of microbial agents, especially make adaptive adjustments to heavy metal pollutants (such as lead, mercury, and cadmium) in the water.

[0101] Treatment of by-products during degradation:

[0102] The intermediates and by-products produced during the floc degradation process are monitored through regular water sampling and analysis.

[0103] The by-products produced are treated by physical filtration, chemical neutralization or further biodegradation to prevent secondary pollution.

[0104] Degradation effect evaluation:

[0105] Degradation effect: The floc degradation effect was confirmed through water quality assessment, and the floc removal rate was expected to be 60% to 80%.

[0106] Water quality improvement: The organic matter concentration in the water body is expected to decrease by 50%-70%, the dissolved oxygen concentration will be stabilized at above 5 mg / L, and the pH value will be maintained between 6.5 and 8.5.

[0107] Ecological impact: By monitoring the changes in the structure of microbial communities, we ensure that the application of microbial agents will not cause an imbalance in the water ecosystem, and that the water quality and ecological environment of the reservoir remain stable.

[0108] result:

[0109] The microbial technology reservoir floc degradation method of this embodiment has achieved remarkable results in the reservoir, with the floc removal rate reaching the expected 70%, the water quality being significantly improved, and the microbial community stability being good, without ecological imbalance. Long-term effect monitoring shows that after the addition of microbial agents, the floc degradation efficiency in the water body continues to maintain a high level, without degradation effect decline or by-product accumulation problems.

[0110] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for degrading reservoir flocs based on microbial technology, characterized in that: The following steps are involved: a. Conduct a comprehensive water quality analysis of the reservoir water, including the determination of organic matter concentration, floc quantity, pH value, dissolved oxygen content, and temperature parameters, to determine the cause and distribution characteristics of floc in the reservoir, and use high-throughput sequencing technology to analyze the existing microbial community structure in the water body, identify key microbial species and their functions, so as to optimize the formulation of subsequent microbial agents; b. Cultivate aerobic bacteria, anaerobic bacteria and auxiliary microbial strains under sterile conditions, and amplify each strain through fermentation technology to ensure its activity and degradation ability, and mix each component according to the following mass percentage: Aerobic strains, accounting for 40% of the total amount of the inoculum, including 20% ​​of Pseudomonas and 20% of Bacillus; Anaerobic bacteria, accounting for 30% of the total amount of the bacterial agent, including 15% of methanogens and 15% of sulfate-reducing bacteria; Auxiliary microorganisms, accounting for 15% of the total amount of the inoculant, including 10% of fungi and 5% of enzyme-producing bacteria; Nutritional additives, accounting for 10% of the total amount of the inoculant, including 4% amino acid mixture, 3% potassium dihydrogen phosphate, and 3% iron, zinc, and magnesium in total; Protective agent, accounting for 5% of the total amount of the microbial agent, including 3% glycerol and 2% sucrose; Carrier materials, accounting for 10% of the total amount of the microbial agent, including 5% bentonite and 5% activated carbon; The above ingredients are fully mixed under sterile conditions to evenly distribute the microorganisms and nutrients on the carrier material, and freeze-dried to extend the shelf life of the bacterial agent and facilitate transportation and storage; c. Determine the dosage of the microbial agent based on the water organic matter concentration, flocculent quantity and water volume obtained in the pretreatment and assessment steps, and evenly distribute the prepared microbial agent in the reservoir water to ensure uniform distribution and effective contact of the microorganisms in the water; d. Install temperature sensors, pH sensors and dissolved oxygen sensors in the reservoir to monitor water environment parameters in real time through an automated control system; e. Collect water samples regularly, use high-throughput sequencing technology and water quality analysis methods to monitor the microbial community structure, floc residue and organic matter degradation rate, and adjust the dosage and strain ratio of microbial agents in a timely manner according to the monitoring results to avoid significant changes in the microbial community structure and maintain the ecological balance of the reservoir; f. In the process of floc degradation, intermediate products or by-products are generated, which are monitored through water sampling and analysis, and the by-products produced by degradation are treated by physical filtration, chemical neutralization or further biodegradation methods to prevent secondary pollution; g. After the degradation process is completed, conduct a comprehensive water quality assessment of the water body to confirm the flocculent degradation effect and the degree of water quality improvement, prepare a detailed project evaluation report, and record the changes in various parameters, degradation efficiency and ecological impact during the degradation process as a basis for subsequent optimization and reference; h. Through automated control systems and regular monitoring, abnormal environmental parameters or unexpected problems during the degradation process can be discovered in a timely manner. According to the preset emergency plan, rapid adjustments to the dosage, suspension of dosage or other remedial measures can be taken to ensure the safety and stability of the degradation process.

2. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The auxiliary microorganisms include fungi and enzyme-producing bacteria, which are used to enhance the degradation efficiency of floccules by decomposing complex organic polymers and secreting a variety of enzymes. The protective agent is selected from glycerol and sucrose, which is used to improve the survival rate and adaptability of microbial agents in water bodies.

3. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The dosage of the microbial agent is adjusted according to the total volume of the reservoir water and the amount of flocs. The dosage of the microbial agent is 1 kg to 5 kg per cubic meter of water, and is added in stages and batches through an automated dosing device to ensure uniform distribution of microorganisms and not cause ecological imbalance in the water body.

4. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The microbial inoculant is stored in sealed packaging to prevent the external environment from affecting the activity of the inoculant. The sealed packaging material is a food-grade moisture-proof and oxidation-resistant material.

5. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: During the addition process of the microbial agent, multiple microbial distribution monitoring points are set in the reservoir, and data is transmitted to the control center in real time through a wireless sensor network, thereby achieving accurate addition of microorganisms and position optimization.

6. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The enzyme-producing bacteria in the microbial agent include β-glucosidase, lipase and protease, wherein β-glucosidase is used to decompose cellulose substances, lipase decomposes fat pollutants, and protease is used to degrade protein organic matter in water.

7. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The water body temperature is between 20°C and 30°C, the pH value is between 6.5 and 8.5, and the dissolved oxygen concentration is above 5 mg / L to maintain the optimal activity of the microorganisms.

8. The method for degrading reservoir flocs based on microbial technology according to claim 1, characterized in that: The water quality analysis further analyzes the heavy metal pollutants in the reservoir water, detects the lead, mercury, and cadmium heavy metals in the water by high performance liquid chromatography or atomic absorption spectrometry, and adjusts the ratio of microbial agents according to the test results to increase the degradation or adsorption capacity of these heavy metals.

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