Hydroponic nutrient solution containing growth regulator and hydroponic method for grape cuttage

Abstract

The invention provides a water culture nutrient solution containing a growth regulator and a water culture method for grape cuttage, and belongs to the technical field of plant growth regulators. The hydroponic nutrient solution provided by the invention is prepared from the following components in mass concentration: 1.0 to 1.5 g / L of 1 / 2 Hoagland, 20 to 30 g / L of cane sugar, 0.4 to 0.6 g / L of indoleacetic acid, 2.5 to 3.5 g / L of indolebutyric acid, 0.8 to 1.2 g / L of naphthylacetic acid and 1.8 to 2.2 mg / L of zeatin. Indoleacetic acid, indolebutyric acid, naphthylacetic acid and zeatin in the hydroponic nutrient solution can synergistically promote rooting of grape cuttings, and the rooting rate reaches up to 98%. The preparation method of the hydroponic nutrient solution is simple and convenient, and is suitable for large-scale popularization and application.

Description

Technical Field

[0001] The invention relates to the technical field of plant growth regulators, in particular to a hydroponic nutrient solution containing a growth regulator and a hydroponic method for grape cuttings. Background Art

[0002] Grapes (Vitis vinifera L.) are vine plants belonging to the genus Vitis in the family Vitaceae. They have high nutritional value and are mainly divided into two types: table grapes and wine grapes. From 2012 to 2021, grape production increased from 10.006 million tons to 14.998 million tons, with an average annual compound growth rate of 4.6%. The current grape industry has entered a critical period of deep adjustment to reduce area, stabilize production, improve quality, and strengthen the market. Innovative grape production technology is an important part of achieving the upgrading of the grape industry.

[0003] Grape propagation includes sexual propagation (such as sowing propagation, seed propagation) and asexual propagation (such as cuttings, layering, grafting). Since grapes are cross-pollinated under normal circumstances, their seeds are mostly natural hybrids, which cannot maintain the characteristics of their mother plants, and the consistency of the group is very poor. Therefore, seed propagation is generally only used when breeding new varieties. In the process of propagating grape seedlings, asexual propagation is generally used. Cuttings are cuttings taken from one-year-old branches of the mother plant, which are rooted under suitable conditions and grow into self-rooted seedlings like the mother plant. Cuttings can also use non-lignified branches of the current year (green branches), that is, green branch cuttings. Cuttings are the simplest and most effective asexual propagation method, and are widely used in production areas without phylloxera damage. Traditional cuttings generally use loose, fertile, high-temperature, moist or easily irrigated soil as cutting soil. Dry and rocky soils have poor rooting of cuttings, and seedlings grow slowly and difficultly; soils that have been used to grow oaks, chestnuts, and other trees may cause grape root rot; calcareous soils lack iron ions that can be absorbed by plants, often causing chlorosis in seedlings. Therefore, grape cutting seedlings have high requirements for soil quality and soil microbial conditions.

[0004] Hydroponics is one of the main types of soilless cultivation. Hydroponics is a cultivation method that uses specific facilities to create a relatively closed environment that can store nutrient solution, and keeps the water, fertilizer, air, heat and other conditions required for root growth relatively stable, with all or part of the root system "immersed" in the nutrient solution. Hydroponics isolates seedlings from the interference of soil pests, and its suitable physical and chemical environment is more suitable for plant growth and development. Compared with soil and substrate cultivation, hydroponics absorbs water and fertilizer more directly and efficiently. Hydroponics seedlings are widely used in the field of vegetable cultivation, but there is little research on grape cutting seedlings. Summary of the invention

[0005] The purpose of the invention is to provide a hydroponic nutrient solution containing a growth regulator and a hydroponic method for grape cuttings, so as to fill the gap in the field of grape hydroponic cutting seedling cultivation.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides a hydroponic nutrient solution containing a growth regulator, comprising the following components in mass concentrations: 1.0-1.5 g / L of 1 / 2 Hoagland, 20-30 g / L of sucrose, 0.4-0.6 g / L of indoleacetic acid, 2.5-3.5 g / L of indolebutyric acid, 0.8-1.2 g / L of naphthaleneacetic acid and 1.8-2.2 mg / L of zeatin.

[0008] The invention also provides application of the hydroponic nutrient solution in grape cutting seedling cultivation.

[0009] The present invention also provides a hydroponic method for grape cuttings, comprising the following steps:

[0010] S1. Select grapevines with good growth and yield performance as mother plants;

[0011] S2. Cut off the lignified branches with a diameter of 8 to 14 mm from the mother plant;

[0012] S3. Cut off branches with diameters less than 3.5 mm and greater than 14 mm, cut into 2-3-node-long segments, and then disinfect;

[0013] S4. Prune the disinfected branch segments, retain 1 to 2 buds, cut the bottom of the cuttings flat, and obtain the cuttings; immerse the lower end of the cuttings in the hydroponic nutrient solution, and after culturing for 25 to 30 days, transplant the grape seedlings.

[0014] Preferably, the grapevine is a non-toxic mature grapevine.

[0015] Preferably, a carbendazim solution is used for disinfection, and the concentration of the carbendazim solution is 0.8 to 1.2 g / L.

[0016] Preferably, the length of the cuttings immersed in the hydroponic nutrient solution is 2.5 to 3.5 cm.

[0017] Preferably, the culture conditions are as follows: relative humidity is 78-82%, the temperature of the hydroponic nutrient solution is 25° C.-30° C., and the light intensity is 1500-2500 Lux.

[0018] Preferably, the cutting seedlings are transplanted when they meet the following conditions: number of roots ≥ 8, average root length ≥ 5 cm.

[0019] Preferably, the variety of the grapevine includes "Sunshine Rose" grapes.

[0020] By adopting the above technical scheme, the present invention has the following beneficial effects: the hydroponic nutrient solution of the present invention comprises the following components in mass concentration: 1.0-1.5 g / L of 1 / 2 Hogland, 20-30 g / L of sucrose, 0.4-0.6 g / L of indoleacetic acid, 2.5-3.5 g / L of indolebutyric acid, 0.8-1.2 g / L of naphthaleneacetic acid and 1.8-2.2 mg / L of zeatin. Indoleacetic acid, indolebutyric acid, naphthaleneacetic acid and zeatin in the hydroponic nutrient solution of the present invention can synergistically promote the rooting of grape cuttings, with a rooting rate of up to 98%. The preparation method of the hydroponic nutrient solution of the present invention is simple and suitable for large-scale promotion and application. DETAILED DESCRIPTION

[0021] The invention provides a hydroponic nutrient solution containing a growth regulator, comprising the following components in mass concentrations: 1.0-1.5 g / L of 1 / 2 Hoagland, 20-30 g / L of sucrose, 0.4-0.6 g / L of indoleacetic acid, 2.5-3.5 g / L of indolebutyric acid, 0.8-1.2 g / L of naphthaleneacetic acid and 1.8-2.2 mg / L of zeatin.

[0022] In the hydroponic nutrient solution of the present invention, the mass concentration of 1 / 2 Hoagland is 1.0 to 1.5 g / L, more preferably 1.2 to 1.3 g / L, and more preferably 1.26 g / L;

[0023] The mass concentration of the sucrose is 20 to 30 g / L, more preferably 23 to 27 g / L, and more preferably 25 g / L;

[0024] The mass concentration of the indoleacetic acid (IAA) is 0.4 to 0.6 g / L, more preferably 0.45 to 0.55 g / L, and more preferably 0.5 g / L;

[0025] The mass concentration of the indolebutyric acid (IBA) is 2.5 to 3.5 g / L, more preferably 2.8 to 3.2 g / L, and more preferably 3 g / L;

[0026] The mass concentration of naphthaleneacetic acid (NAA) is 0.8 to 1.2 g / L, more preferably 0.9 to 1.1 g / L, and more preferably 1 g / L;

[0027] The mass concentration of zeatin (ZT) is 1.8 to 2.2 mg / L, more preferably 1.9 to 2.1 mg / L, and even more preferably 2 mg / L.

[0028] The present invention has no special limitation on the sources of the above materials, and conventional commercially available products well known to those skilled in the art can be used. Indolebutyric acid, naphthylacetic acid and zeatin in the hydroponic nutrient solution are cytokinins, which can promote the differentiation of cells at the base of grape cuttings; indoleacetic acid is an auxin, which can promote rooting and increase the length of adventitious roots. The four plant growth regulators have a synergistic effect on the rooting of grape cuttings; and sucrose provides a stable carbon source for the cuttings.

[0029] The preparation method of the nutrient solution is as follows: after the corresponding mass of indoleacetic acid, indolebutyric acid, naphthylacetic acid and zeatin are completely dissolved in ethanol, 1 / 2 Hoagland and sucrose are added and stirred until completely dissolved, and then the volume is fixed and the pH value is adjusted to between 5.8 and 6.0 to obtain the nutrient solution. The volume concentration of the ethanol is 93-97%, more preferably 94-96%, and more preferably 95%.

[0030] The invention also provides application of the hydroponic nutrient solution in grape cutting seedling cultivation.

[0031] The present invention also provides a hydroponic method for grape cuttings, comprising the following steps:

[0032] S1. Select grapevines with good growth and yield performance as mother plants;

[0033] S2. Cut off the lignified branches with a diameter of 8 to 14 mm from the mother plant;

[0034] S3. Cut off branches with diameters less than 3.5 mm and greater than 14 mm, cut into 2-3-node-long segments, and then disinfect;

[0035] S4. Prune the disinfected branch segments, retain 1 to 2 buds, cut the bottom of the branch flat, and obtain cuttings; immerse the lower end of the cuttings in the hydroponic nutrient solution, and after culturing for 25 to 30 days, transplant the grape seedlings.

[0036] The present invention first selects grapevines with good growth and yield performance as mother plants, and the grapevines are non-toxic mature grapevines; then, lignified branches on the mother plants are cut, and the diameter of the lignified branches is 8-14 mm, more preferably 10-12 mm, and more preferably 11 mm; the branches need to be of moderate thickness and have a standard thickness of the variety, and the parts of the branches with diameters less than 3.5 mm and greater than 14 mm are cut off, and the tendrils and side shoots are cut off at the same time, and the branches are cut into 2-3 node-long branch segments, and the length of the branch segments is 37-43 cm, more preferably 39-42 cm, and more preferably 40 cm; the varieties of the grapevines include "Sunshine Rose" grapes.

[0037] In the present invention, the pruned branches are bundled into a bundle, rinsed with running water, and then disinfected with a carbendazim solution; preferably, 50 to 100 branches are bundled into a bundle, more preferably 70 to 80 branches, and more preferably 75 branches; the time for running water rinsing is 25 to 35 minutes, more preferably 27 to 32 minutes, and more preferably 30 minutes. After the branches are rinsed with running water, the grape branches are immersed in a carbendazim solution for disinfection, the mass concentration of the carbendazim solution is 0.8 to 1.2 g / L, more preferably 0.9 to 1.1 g / L, and more preferably 1 g / L, and the time for disinfection with the carbendazim solution is 1.5 to 2.5 hours, and more preferably 2 hours.

[0038] In the present invention, the disinfected branch segments are pruned, 1 to 2 buds are retained, and the bottom of the cuttings is cut flat to obtain the cuttings. The function of this step is to ensure that the base of each cutting is flush and can fully contact the nutrient solution, and secondly, to create new wounds and improve the rooting rate. Then the lower end of the cutting is immersed in the hydroponic nutrient solution, and the length of the cutting immersed in the hydroponic nutrient solution is 2.5 to 3.5 cm, more preferably 2.8 to 3.2 cm, and more preferably 3 cm. The cultivation of the cuttings is preferably carried out on a cutting seedling bed. After culturing for 25 to 30 days, the cutting seedlings are obtained and transplanted; the culturing conditions are as follows: the relative humidity is 78 to 82%, more preferably 79 to 81%, and more preferably 80%; the temperature of the hydroponic nutrient solution is 25°C to 30°C, more preferably 26 to 29°C, and more preferably 27°C; the light intensity is 1500 to 2500 Lux, more preferably 1800 to 2300 Lux, and more preferably 2000 Lux. In the present invention, the cutting seedlings are transplanted when they meet the following conditions: the number of roots is ≥8, and the average root length is ≥5 cm.

[0039] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0040] In the embodiment of the present invention, the hydroponic cutting seedling bed consists of two parts: a seedling bed and a cutting board. Among them, the seedling bed is made of lightweight material and is shaped like a trough. Water storage tanks are provided at both ends, and circular holes are opened at the upper and lower parts of the water storage tanks, which are used as water inlets and outlets. The circular hole at the upper part of the water storage tank is the water inlet, and the circular hole at the lower part of the water storage tank is the water outlet. A plurality of diversion grooves are provided in the middle of the seedling bed, and the water storage tank and the diversion grooves are separated by a baffle with a water permeable opening. The lower part of the baffle water permeable opening is 5 cm high, so that the water level inside the diversion groove is about 3 to 5 cm. Partitions are used to separate the diversion grooves. The cutting board is made of polyethylene foam board, and several slits are opened for cuttings. The slit is opposite to the middle of the diversion groove of the seedling bed. A uniform heating device is provided at the bottom of the hydroponic cutting seedling bed, which can control the water temperature of the nutrient solution to be stable at 25 to 30°C.

[0041] The 1 / 2 Hoagland effective ingredients in the embodiments of the present invention are preferably as shown in Table 1.

[0042] Table 11 / 2 Hogland nutrient solution formula

[0043] Element name Concentration (mg / L) <![CDATA[K2SO4]]> Potassium sulfate 303.5 <![CDATA[NH4H2PO4]]> Ammonium dihydrogen phosphate 57.5 <![CDATA[MgSO4]]> Magnesium sulfate 246.5 <![CDATA[C 10 H 12 FeN2NaO8]]> Sodium Ferric EDTA 10 <![CDATA[FeSO4]]> Ferrous Sulfate 7.5 <![CDATA[H3BO3]]> Boric acid 1.43 <![CDATA[Na2B4O7·10H2O]]> Borax 2.25 <![CDATA[MnSO4]]> Manganese sulfate 1.07 <![CDATA[CuSO4]]> Copper sulfate 0.03 <![CDATA[ZnSO4]]> Zinc sulfate 0.11 <![CDATA[(NH4)2SO4]]> Ammonium sulfate 0.01

[0044] Example 1

[0045] The hydroponic nutrient solution in this embodiment is composed of the following components in mass concentrations: 1 / 2 Hoagland 1.26 g / L, sucrose 25 g / L, indoleacetic acid 0.5 g / L, indolebutyric acid 3 g / L, naphthylacetic acid 1 g / L and zeatin 2 mg / L.

[0046] Cut off the lignified branches with a diameter of 8 to 14 mm from the mature "Sunshine Rose" grape mother plants with good growth and yield. Cut off the branches with a diameter less than 3.5 mm and greater than 14 mm, and cut off the tendrils and side shoots, and then cut into 2 to 3 sections (about 40 cm); bundle 50 branches into a bundle; rinse with running water for 30 minutes and disinfect with 1g / L carbendazim.

[0047] Keep 1 to 2 buds on the disinfected branch segments, cut the bottom flat, and obtain cuttings. Evenly insert 100 cuttings into the cutting seedling bed, ensure that the base is immersed in the nutrient solution for 3 to 5 cm, and the distance between two adjacent cuttings is 10 cm for cultivation. Maintain the relative humidity of the environment at 80%, the temperature at 25℃ to 30℃, and the light intensity at 1500 to 2500 Lux. Remove diseased plants, rotten plants, and fallen leaves in time during the cultivation process. After 25 to 30 days of cutting, when the number of roots of the cuttings is ≥8 and the average root length is ≥5 cm, transplant them.

[0048] Example 2

[0049] The hydroponic nutrient solution in this embodiment is composed of the following components in mass concentrations: 1 / 2 Hoagland 1.0 g / L, sucrose 20 g / L, indoleacetic acid 0.4 g / L, indolebutyric acid 2.5 g / L, naphthylacetic acid 0.8 g / L and zeatin 1.8 mg / L.

[0050] Cut off the lignified branches with a diameter of 8 to 14 mm from the mature "Sunshine Rose" grape mother plants with good growth and yield. Cut off the branches with a diameter less than 3.5 mm and greater than 14 mm, and cut off the tendrils and side shoots, and then cut into 2 to 3 sections (about 40 cm); bundle 50 branches into a bundle; rinse with running water for 30 minutes and disinfect with 0.8g / L carbendazim.

[0051] Keep 1 to 2 buds on the disinfected branch segments, cut the bottom flat, and obtain cuttings. Evenly insert 100 cuttings into the cutting seedling bed, ensure that the base is immersed in the nutrient solution for 3 to 5 cm, and the distance between two adjacent cuttings is 10 cm for cultivation. Maintain the relative humidity of the environment at 78 to 82%, the temperature at 25℃ to 30℃, and the light intensity at 1500 to 2500 Lux. During the cultivation process, remove diseased plants, rotten plants, and fallen leaves in time. After 25 to 30 days of cutting, when the number of roots of the cuttings is ≥8 and the average root length is ≥5 cm, transplant them.

[0052] Example 3

[0053] The hydroponic nutrient solution in this embodiment is composed of the following components in mass concentrations: 1 / 2 Hoagland 1.5 g / L, sucrose 30 g / L, indoleacetic acid 0.6 g / L, indolebutyric acid 3.5 g / L, naphthylacetic acid 1.2 g / L and zeatin 2.2 mg / L.

[0054] Cut off the lignified branches with a diameter of 8 to 14 mm from the mature "Sunshine Rose" grape mother plants with good growth and yield. Cut off the branches with a diameter less than 3.5 mm and greater than 14 mm, and cut off the tendrils and side shoots, and then cut into 2 to 3 sections (about 40 cm); bundle 50 branches into a bundle; rinse with running water for 30 minutes and disinfect with 1.2 g / L carbendazim.

[0055] Keep 1 to 2 buds on the disinfected branch segments, cut the bottom flat, and obtain cuttings. Evenly insert 100 cuttings into the cutting seedling bed, ensure that the base is immersed in the nutrient solution for 3 to 5 cm, and the distance between two adjacent cuttings is 10 cm for cultivation. Maintain the relative humidity of the environment at 78 to 82%, the temperature at 25℃ to 30℃, and the light intensity at 1500 to 2500 Lux. During the cultivation process, remove diseased plants, rotten plants, and fallen leaves in time. After 25 to 30 days of cutting, when the number of roots of the cuttings is ≥8 and the average root length is ≥5 cm, transplant them.

[0056] Comparative Example 1

[0057] Different from Example 1, the hydroponic nutrient solution of Comparative Example 1 consists of the following components in mass concentrations: 1 / 2 Hoagland 1.26 g / L, sucrose 25 g / L, indoleacetic acid 0.5 g / L, and the other steps are the same as in Example 1.

[0058] Comparative Example 2

[0059] Different from Example 1, the hydroponic nutrient solution of Comparative Example 2 consists of the following components in mass concentrations: 1 / 2 Hoagland 1.26 g / L, sucrose 25 g / L, indolebutyric acid 3 g / L, and the other steps are the same as in Example 1.

[0060] Comparative Example 3

[0061] Different from Example 1, the hydroponic nutrient solution of Comparative Example 3 consists of the following components in mass concentrations: 1 / 2 Hoagland 1.26 g / L, sucrose 25 g / L, and naphthaleneacetic acid 1 g / L, and the other steps are the same as those in Example 1.

[0062] Comparative Example 4

[0063] Different from Example 1, the hydroponic nutrient solution of Comparative Example 4 consists of the following components in mass concentrations: 1 / 2 Hoagland 1.26 g / L, sucrose 25 g / L, zeatin 2 mg / L, and the other steps are the same as Example 1.

[0064] Comparative Example 5

[0065] The difference from Example 1 is that the concentration of indoleacetic acid in the hydroponic nutrient solution of Comparative Example 5 is 0.1 g / L, and the other steps are the same as those of Example 1.

[0066] Comparative Example 6

[0067] The difference from Example 1 is that the concentration of indoleacetic acid in the hydroponic nutrient solution of Comparative Example 6 is 1 g / L, and the other steps are the same as those of Example 1.

[0068] Comparative Example 7

[0069] Different from Example 1, the concentration of indolebutyric acid in the hydroponic nutrient solution of Comparative Example 7 is 1.5 g / L, and the other steps are the same as those of Example 1.

[0070] Comparative Example 8

[0071] Different from Example 1, the concentration of indolebutyric acid in the hydroponic nutrient solution of Comparative Example 7 is 4.5 g / L, and the other steps are the same as those of Example 1.

[0072] Comparative Example 9

[0073] Different from Example 1, the concentration of naphthylacetic acid in the hydroponic nutrient solution of Comparative Example 9 is 0.5 g / L, and the other steps are the same as those of Example 1.

[0074] Comparative Example 10

[0075] Different from Example 1, the concentration of naphthylacetic acid in the hydroponic nutrient solution of Comparative Example 10 is 1.5 g / L, and the other steps are the same as those of Example 1.

[0076] Comparative Example 11

[0077] The difference from Example 1 is that the concentration of zeatin in the hydroponic nutrient solution of Comparative Example 11 is 1 mg / L, and the other steps are the same as those of Example 1.

[0078] Comparative Example 12

[0079] Different from Example 1, the concentration of zeatin in the hydroponic nutrient solution of Comparative Example 12 is 3 mg / L, and the other steps are the same as those of Example 1.

[0080] Blank group: Different from Example 1, indoleacetic acid, indolebutyric acid, naphthylacetic acid and zeatin were not added to the blank group, and other steps were the same as those in Example 1.

[0081] After the cuttings were cultured for 21 days, the rooting rate, average number of roots and average root length of the cuttings of Examples 1 to 3, Comparative Examples 1 to 12 and the blank group were statistically analyzed. The results are shown in Table 2.

[0082] Rooting rate = number of rooted cuttings / total number of cuttings × 100%; average rooting number = total number of roots of rooted cuttings / number of rooted cuttings. The statistics of rooting rate and average rooting number are both calculated by professional and technical personnel.

[0083] Table 2 Rooting of cuttings

[0084] Group Rooting rate (%) Average number of roots Average root length (cm) Example 1 98 7.38 5.86 Example 2 98 7.19 5.25 Example 3 97 7.16 5.21 Comparative Example 1 25 3.08 3.37 Comparative Example 2 28 3.21 3.28 Comparative Example 3 21 2.85 3.32 Comparative Example 4 12 2.50 2.71 Comparative Example 5 73 5.21 4.23 Comparative Example 6 78 6.00 4.59 Comparative Example 7 76 5.53 4.38 Comparative Example 8 77 5.36 4.03 Comparative Example 9 83 5.48 4.49 Comparative Example 10 80 6.28 4.16 Comparative Example 11 88 5.80 4.26 Comparative Example 12 87 5.97 4.43 Blank Group 52 1.85 1.50

[0085] As shown in Table 2, the rooting rate, average root number and average root length of the grape cuttings in Examples 1 to 3 are significantly better than those in Comparative Examples 1 to 12 and the blank group, indicating that the hydroponic nutrient solution of the present invention can effectively promote the rooting of grape cuttings. As shown in Comparative Examples 1 to 4, the promoting effect of indoleacetic acid, indolebutyric acid, naphthylacetic acid and zeatin on the rooting of grape cuttings is greater than the sum of the promoting effects of indoleacetic acid, indolebutyric acid, naphthylacetic acid and zeatin on the rooting of grape cuttings alone, indicating that indoleacetic acid, indolebutyric acid, naphthylacetic acid and zeatin in the hydroponic nutrient solution can synergistically promote the rooting of grape cuttings.

[0086] From the above examples, it can be seen that the present invention provides a hydroponic nutrient solution containing a growth regulator and a hydroponic method for grape cuttings. Indoleacetic acid, indolebutyric acid, naphthylacetic acid and zeatin in the hydroponic nutrient solution of the present invention can synergistically promote the rooting of grape cuttings, with a rooting rate of up to 98%.

[0087] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A hydroponic nutrient solution containing a growth regulator, characterized in that: The invention comprises the following components in mass concentrations: 1.0-1.5 g / L of 1 / 2 Hoagland, 20-30 g / L of sucrose, 0.4-0.6 g / L of indoleacetic acid, 2.5-3.5 g / L of indolebutyric acid, 0.8-1.2 g / L of naphthylacetic acid and 1.8-2.2 mg / L of zeatin.

2. Application of the hydroponic nutrient solution according to claim 1 in grape cutting seedling cultivation.

3. A hydroponic method for grape cuttings, characterized in that: The following steps are involved: S1. Select grapevines with good growth and yield performance as mother plants; S2. Cut off the lignified branches with a diameter of 8 to 14 mm from the mother plant; S3. Cut off branches with diameters less than 3.5 mm and greater than 14 mm, cut into 2-3-node-long segments, and then disinfect; S4. Prune the disinfected branch segments, retain 1 to 2 buds, cut the bottom of the cuttings flat, and obtain cuttings; immerse the lower end of the cuttings in the hydroponic nutrient solution of claim 1, and after culturing for 25 to 30 days, transplant the grape seedlings.

4. The hydroponic method according to claim 3, characterized in that: The grapevine is a non-toxic mature grapevine.

5. The hydroponic method according to claim 4, characterized in that: A carbendazim solution is used for disinfection, wherein the concentration of the carbendazim solution is 0.8 to 1.2 g / L.

6. The hydroponic method according to claim 5, characterized in that: The length of the cuttings immersed in the hydroponic nutrient solution is 2.5 to 3.5 cm.

7. The hydroponic method according to claim 6, characterized in that: The culture conditions are as follows: relative humidity is 78-82%, the temperature of the hydroponic nutrient solution is 25° C.-30° C., and the light intensity is 1500-2500 Lux.

8. The hydroponic method according to claim 7, characterized in that: Transplantation is carried out when the cutting seedlings meet the following conditions: number of roots ≥ 8, average root length ≥ 5 cm.

9. The hydroponic method according to claim 3, characterized in that: The varieties of the grape vines include "Sunny Rose" grapes.