Micromolecular organic carbon nutrient solution as well as preparation method and application thereof

Abstract

The invention discloses a micromolecular organic carbon nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of agricultural planting. The micromolecular organic carbon nutrient solution is prepared from the following raw materials in parts by weight: 60-80 parts of livestock and poultry liquid manure; 10-30 parts of an organic auxiliary material; 5-10 parts of a core component; the core component consists of acid-producing zymophyte, a functional microbial agent, biological enzyme, free amino acid, a seaweed extract, beta-cyclocitral and medium trace elements. The micromolecular organic carbon nutrient solution provided by the invention is combined with a planting mode of ridging, film mulching and drip irrigation to provide organic nutrition for crops, drip irrigation is applied in a seed germination period and a two-leaf one-core period, a set of integral planting solution is formed, and the bottleneck problems of local soil salinization, poor corn seedling emergence, weak seedling vigor and low yield are solved. The technical measure can effectively reduce the pH and EC value of the soil, improve the physical and chemical properties of the soil, significantly increase the emergence rate of the corn, enhance the growth vigor in the seedling stage and increase the corn yield, and has a great application prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a small molecule organic carbon nutrient solution and a preparation method and application thereof. Background Art

[0002] The climate in the Hetao Irrigation Area of ​​Inner Mongolia is dry, with little precipitation and saline-alkali soil. With the increase in irrigation area and the reduction in the amount of water diverted from the Yellow River in recent years, the soil has evaporated strongly, causing salt to accumulate on the surface and serious secondary salinization of the soil. Some soils have been forced to be abandoned due to severe salinization, which seriously restricts the sustainable development of the agricultural economy in the irrigation area. The traditional method of reducing soil salinity is to drain the salt from the soil through flooding, but this method cannot achieve the purpose of improvement for soils with heavy clay and extremely low water conductivity.

[0003] Rational use of salinized soil and prevention of secondary salinization of farmland are important research contents to ensure the sustainable use of land resources in arid and semi-arid areas. The saline-alkali area is dry and water-scarce, and water-saving and efficient improvement of saline-alkali land is one of the main technical difficulties. Drip irrigation is a point source diffusion method for corn drip irrigation under film in saline-alkali land in the Hetao Irrigation Area. It has the characteristics of long time, small flow and high frequency. A desalination zone is formed under the dripper. Salt mainly accumulates at the edge of the moist front, creating good conditions for crop growth and development. The mulch film basically blocks the channel for soil evaporation to the atmosphere and weakens the upward movement of soil salt. While effectively leaching soil salt, mulch drip irrigation inhibits soil surface evaporation and reduces salt upward movement, thereby achieving the goals of water saving, steam reduction and salt control. It can regulate the surface soil temperature, reduce the temperature difference between day and night in the soil layer, improve water use efficiency, reduce ineffective evaporation and heat loss, improve soil water and heat conditions, and effectively inhibit soil salt surface accumulation and prevent secondary salinization while effectively leaching salt. The combination of mulching measures and ridge farming methods can promote crop growth, increase temperature and moisture retention, and improve crop water utilization. Therefore, ridge mulching and drip irrigation technology is a better technical measure for improving and developing saline-alkali land.

[0004] In the field production process, ensuring a high emergence rate is the basis for obtaining high crop yields. There are many factors that affect emergence, including temperature, moisture, salinity, soil structure, crop types, etc. The spring in the irrigation area is dry and windy, the soil temperature is low, and the harm of salinity and alkali causes weak corn seedlings and low yields. Many studies have shown that during the entire growth period of crops, the emergence stage is the most sensitive period to salt. Salt stress, as one of the main abiotic stresses, can affect the normal metabolism of plants by destroying the structure and function of plant cells, destroying the permeability of biological membranes, affecting enzyme activity, reducing photosynthetic rate, etc., resulting in limited crop vegetative growth and reproductive growth, and ultimately leading to reduced crop yield and quality. In the actual application of improving saline-alkali soil, only inorganic components are usually used. The improvement effect is not obvious and the unreasonable application not only causes waste of resources and reduced fertilization effect, but also leads to further soil degradation, compaction and environmental pollution.

[0005] Therefore, how to effectively inhibit soil salinity and improve crop resistance and seedling vigor is an urgent need in the current agricultural technology field.

[0006] At present, there are few reports on the research of small molecule organic carbon nutrient solution comprehensive products for desalination and salt suppression in saline soil and improving the growth and stress resistance of crops in the seedling stage under the comprehensive ridge-forming, film-covering and drip irrigation mode of saline-alkali land. In view of this, the present invention is proposed.

[0007] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0008] In order to solve the technical problems of weak growth, low yield and poor quality of corn planted in saline-alkali land in the prior art, a small molecule organic carbon nutrient solution and a preparation method and application thereof are provided.

[0009] The first aspect of the present invention provides a small molecule organic carbon nutrient solution, the raw materials of which are composed of the following components in parts by weight:

[0010] 60-80 parts of liquid livestock and poultry manure;

[0011] 10-30 parts of organic excipients;

[0012] 5-10 servings of core ingredients;

[0013] The core components are composed of acid-producing fermentation bacteria, functional bacterial agents, biological enzymes, free amino acids, seaweed extracts, β-cyclocitral, and trace elements. The particle size of the solid particles does not exceed 200 meshes.

[0014] Preferably, the weight ratio of each component in the core composition is: acid-producing fermentation bacteria: functional bacterial agent: biological enzyme: free amino acid: seaweed extract: β-cyclocitral: trace elements = 10-20:10-20:10-20:2-10:2-10:2-10:1-5;

[0015] More preferably, the weight ratio of the components in the core ingredients is: acid-producing fermentation bacteria: functional bacterial agent: biological enzyme: free amino acid: seaweed extract: β-cyclocitral: trace elements = 12-18:12-18:12-18:5-8:5-8:5-8:2-4.

[0016] In the present invention, the core component is used as a regulating substance, and its characteristics are that it can reduce soil pH and EC value, improve the physical and chemical properties of saline-alkali soil, increase soil enzyme activity, activate microbial activity, increase the content of carbon monoxide, water-soluble organic carbon nitrogen, ammonium nitrogen and nitrate nitrogen in the soil, increase soil soluble organic nutrients, and improve the utilization efficiency of soil nutrients by plants, thereby alleviating salt-alkali stress, promoting seed germination, improving crop growth quality, and allowing plants to grow better in saline-alkali soil.

[0017] In some embodiments, the organic auxiliary material includes honey and / or brown sugar.

[0018] In some embodiments, the acid-producing fermentation bacteria include one or a combination of acetic acid bacteria, yeast, and lactic acid bacteria; the present invention does not specifically limit the source of the acid-producing fermentation bacteria, and any conventional commercially available acid-producing fermentation bacteria products in the field can be used. In the present invention, the function of the acid-producing fermentation bacteria is to produce stable organic acids by fermentation, effectively reduce the pH value of the fermentation product to 3.5-4.5, and produce substances such as acetic acid, lactic acid, citric acid and malic acid, acidify and alkalize the soil for repair; the colloidal substances produced during the fermentation process can combine with soil particles to form a relatively stable soil structure, increase the aeration and water retention of the soil, contribute to the growth and development of plant roots, and improve the stress resistance of plants.

[0019] In some embodiments, the functional bacterial agent is a microbial agent with the functions of phosphorus solubilization, potassium solubilization, nitrogen fixation, and rot promotion, including one or more combinations of Bacillus, Streptomyces jingyangensis, mycorrhizal fungi, and brown nitrogen-fixing bacteria, and the Bacillus includes one or more combinations of Bacillus licheniformis, Bacillus megaterium, Bacillus colloidus, Bacillus jelly-like, Bacillus subtilis, and Bacillus thuringiensis; the present invention does not specifically limit the source of the functional bacterial agent, and any conventional commercially available functional bacterial agent products in the field can be used. In the present invention, the function of the functional bacterial agent is to promote the dissolution and release of insoluble nutrients in the soil through nitrogen fixation, phosphorus solubilization, and potassium solubilization, thereby improving the nutritional status of the soil and improving the fertility of the soil. The functional bacteria can effectively decompose the macromolecular structure of organic matter into small molecular structures, so that plants can fully absorb the nutrients of organic matter and improve the nutrient supply capacity of crops. The functional bacteria can produce bioactive substances such as gibberellins, cytokinins, and indoleacetic acid in life activities, which can effectively stimulate, regulate and promote the growth and development of crops, and improve the yield and quality of crops.

[0020] In some embodiments, the biological enzyme includes one or a combination of cellulase, protease, superoxide dismutase, and hydrolase; the present invention does not specifically limit the source of the biological enzyme for synthesizing the biostimulant, and any conventional commercially available biological enzyme product in the art can be used. In the present invention, the role of the biological enzyme is to accelerate the transportation of nutrients in the plant body, catalyze the conversion rate of nutrients in the plant body, and accelerate the metabolism of the plant. Supplementing biological enzymes for plants inhibits and kills harmful bacteria, controls the secretion of toxins, improves plant resistance, and maintains plant health.

[0021] In some embodiments, the free amino acids include one or a combination of glycine, glutamic acid, arginine, leucine, proline, γ-aminobutyric acid, and β-alanine; the present invention does not specifically limit the source of the free amino acids, and any free amino acid products conventionally available on the market in the art can be used. In the present invention, the free amino acids act to enhance root development, increase plant tolerance to osmotic stress, improve plant stress resistance and pollen vitality, and improve crop resistance to salt stress; adjust soil pH, which helps reduce soil salinity; and promote the activity of soil microorganisms, thereby enhancing soil fertility and water retention capacity.

[0022] In some embodiments, the seaweed extract includes one or a combination of seaweed polysaccharides, alginic acid, betaine, sterols, and mannitol; the present invention does not specifically limit the source of the seaweed extract, and any conventional commercially available seaweed extract product in the field can be used. In the present invention, the role of the seaweed extract is to enhance the tolerance of crops to adversity. Under adverse stress, the seaweed extract can increase the soluble sugar content and the activity of the SOD enzyme. The high biological activity is extremely permeable to the barrier of the plant cell membrane. Through its adsorption, conduction, transport, bridging, slow release, activation and other functions, plant cells can absorb more water and nutrients that were originally inaccessible, reduce the damage of free radicals to the cell membrane, induce crops to produce stress defense, increase the activity of multiple enzymes, and increase the content of phenolic substances, so that the plant's ability to resist salt stress is improved, thereby promoting plant growth and alleviating plant abiotic stress.

[0023] In some embodiments, the β-cyclocitral is a naturally occurring β-carotene-derived carotenoid, which is a natural compound that is inexpensive, effective at low concentrations, and can be applied externally. The molecular formula of β-cyclocitral is C 10 H 16 O, with a molecular weight of 152.2334. The present invention does not particularly limit the source of the β-cyclocitral, and any conventional commercially available β-cyclocitral product in the art can be used. β-Cyclocitral has the effect of promoting plant growth and enhancing the adaptability of plants to stress environments, helping plants to regulate osmotic pressure, maintain ion balance, reduce oxidative stress, or enhance the adaptability of plants to saline-alkali environments. It can promote cell division of root meristems and stimulate the branching of lateral roots, help plants absorb water and nutrients more effectively, and enhance the vitality of plants growing under saline soil conditions, which is of great significance for improving the yield and quality of crops under adverse conditions.

[0024] In some embodiments, the trace elements include one or more of Ca, Mg, B, Fe, Mn, Cu, and Zn. The present invention does not particularly limit the source of the trace elements, and any trace element product conventionally available on the market in the field can be used. In the present invention, the role of the trace elements is to improve the balance of inorganic nutrition in the crop body, avoid fertilizer waste, improve the resistance of crops to stress and disease, improve the ability of crops to resist natural disasters and pests and diseases, improve crop quality, and increase crop yield.

[0025] In some embodiments, the nutrient content of the small molecule organic carbon nutrient solution is:

[0026] Organic matter 140g / L, humic acid 20g / L, macroelements 70g / L, trace elements 25g / L, protease activity ≥550u / mL, cellulase activity ≥35U / mL, crude protein ≥20g / 100g, soluble sugar ≥9.5g / L, free amino acids ≥16.5%, indole-3-acetic acid ≥5000ug / kg, gibberellin ≥20ug / kg, β-cyclocitral ≥55umol / L, effective viable count ≥200 million / mL;

[0027] The pH range of the organic carbon nutrient solution is 3.8-4.6;

[0028] Preferably, the major elements include N, P2O5, and K2O.

[0029] The second aspect of the present invention provides a method for preparing a small molecule organic carbon nutrient solution, which is obtained by fermenting livestock and poultry liquid manure as a main body and adding organic auxiliary materials and core components required for aerobic fermentation.

[0030] In some embodiments, comprising:

[0031] First, add the liquid livestock and poultry manure after solid-liquid separation into a fermentation device, such as a plastic tank or a fermentation tank, add half of the core ingredients in proportion, and turn on the aeration device for internal circulation to ensure that the materials are fully mixed and continue for 5-7 days. Use quicklime to control the pH value in the range of 3.5-5.5 to obtain a mixed solution;

[0032] Then, organic auxiliary materials and the other half of the core ingredients are added to the mixed solution, and the fermentation time is 5-7 days. The pH value is controlled at 4.5 to form a small molecule organic carbon nutrient solution.

[0033] The third aspect of the present invention provides an application of the above-mentioned small molecule organic carbon nutrient solution or the small molecule organic carbon nutrient solution prepared by the above-mentioned preparation method in reducing soil pH and EC value;

[0034] And / or, application in crop cultivation in saline-alkali land.

[0035] And / or, application in improving salt tolerance, stress resistance, and yield of crops in saline-alkali land.

[0036] The fourth aspect of the present invention provides a salt-inhibiting, stress-resistant and seedling-raising planting method. Specifically, the present invention provides a water-saving and efficient improvement of saline-alkali land planting measures using ridge-forming, film-covering and drip irrigation technology, including using ridge-forming, film-covering and drip irrigation technology measures for drip irrigation of the above-mentioned small molecule organic carbon nutrient solution or the small molecule organic carbon nutrient solution prepared by the above-mentioned preparation method during the seed germination period and the two-leaf and one-heart period in the plant planting process. This planting mode is the most effective method, which can increase yield, save water, inhibit salt and reduce deep leakage. On the one hand, the drip irrigation method can have a good leaching effect on salt, and on the other hand, it can more easily maintain the soil around the seeds at a high matrix potential for a long time, thereby compensating for the reduced osmotic potential caused by the excessive salt content in the soil and irrigation water, and creating a favorable environment for seed germination. The film-covering treatment has the best water-retaining and evaporation-inhibiting effect, and has a good salt-pressing effect. On the one hand, it can effectively inhibit the evaporation of soil water, and on the other hand, it can weaken the upward movement trend of salt with water, creating a light salt environment suitable for crop growth. Ridge cultivation technology can change the field micro-topography and increase the surface area, which can effectively increase ventilation and light transmission.

[0037] In some embodiments, the amount of the small molecule organic carbon nutrient solution used for drip irrigation during the seed germination period is 2-7 kg / mu, preferably 5 kg / mu; the amount of the small molecule organic carbon nutrient solution used for drip irrigation during the two-leaf and one-heart period is 5-15 kg / mu, preferably 10 kg / mu, and is dripped in with water during use, generally with a water consumption of 20-30 m3 / mu. 3 / mu.

[0038] The comprehensive technical measures of ridge-forming, film-covering, drip irrigation and small-molecule organic carbon nutrient solution provided by the present invention are applied to agricultural production of corn in saline-alkali land, which can effectively inhibit soil salinity, improve crop stress resistance and seedling vigor, and thus achieve high yield and high quality of crops.

[0039] Compared with the prior art, the technical effects achieved by the present invention are as follows:

[0040] (1) The small molecule organic carbon nutrient solution provided by the present invention has the functions of improving soil, increasing fertilizer efficiency and promoting crop growth and development. It is combined with the planting mode of ridge-forming, film-covering and drip irrigation to provide organic nutrition to crops. It is applied by drip irrigation during the seed germination period and the two-leaf and one-heart period to form a set of overall planting solutions to solve the bottleneck problems of local soil salinization, poor corn emergence, weak seedling vigor and low yield. This technical measure can effectively reduce soil pH and EC values, improve soil physical and chemical properties, significantly increase corn emergence rate, enhance seedling growth and increase corn yield, and has great application prospects.

[0041] (2) The present invention changes the soil moisture distribution and salt migration law by ridge-forming and film-covering, and combines drip irrigation technology to accurately control the supply of water and nutrient solution, so as to at least solve the problem of using small molecule organic carbon nutrient solution to improve soil structure and nutrient status to optimize soil environment under saline-alkali land planting conditions, and cooperate with biotechnology and chemical regulation to effectively inhibit soil salinity, improve crop resistance and seedling vigor, and thus achieve high yield and high quality of crops. DETAILED DESCRIPTION

[0042] The technical solution of the present invention is described below by specific embodiments. It should be understood that one or more steps mentioned in the present invention do not exclude the existence of other methods and steps before and after the combination step, or other methods and steps can be inserted between these explicitly mentioned steps. It should also be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. Unless otherwise specified, the numbering of each method step is only for the purpose of identifying each method step, and does not limit the order of arrangement of each method or limit the scope of implementation of the present invention. The change or adjustment of the relative relationship thereof can also be regarded as the scope of implementation of the present invention without substantial changes in the technical content.

[0043] The sources of the raw materials and instruments used in the examples are not particularly limited and can be purchased from the market or prepared according to conventional methods known to those skilled in the art.

[0044] In one embodiment, the raw materials of the small molecule organic carbon nutrient solution are composed of the following components by weight: 70 parts of livestock and poultry liquid manure, 20 parts of organic auxiliary materials, and 10 parts of core ingredients. The auxiliary material is honey, and the weight ratio of acid-producing fermentation bacteria, functional bacterial agents, biological enzymes, free amino acids, seaweed extracts, β-cyclocitral, and trace elements in the core ingredients is:

[0045] Acid-producing fermentation bacteria: functional bacterial agent: biological enzyme: free amino acid: seaweed extract: β-cyclocitral: trace elements = 15:15:15:6:6:6:3.

[0046] The preparation method of the above-mentioned small molecule organic carbon nutrient solution comprises:

[0047] First, 70 parts of livestock and poultry liquid manure after solid-liquid separation were added to the fermentation tank, half of the core components were added in proportion, and the aeration device was turned on for internal circulation to ensure that the materials were fully mixed and continued for 7 days. Quicklime was used to control the pH value to 4.5 to obtain a mixed solution;

[0048] Then, the organic auxiliary material honey and the other half of the core ingredients are added to the mixed solution, the fermentation time is 7 days, and the pH value is controlled to be 4.5 to form the above-mentioned small molecule organic carbon nutrient solution.

[0049] The nutrient content of the small molecule organic carbon nutrient solution is: organic matter 140g / L, humic acid 20g / L, macroelements 70g / L, trace elements 25g / L, protease activity ≥550U / mL, cellulase activity ≥35U / mL, crude protein ≥20g / 100g, soluble sugar ≥9.5g / L, free amino acids ≥16.5%, indole-3-acetic acid ≥5000ug / kg, gibberellin ≥20ug / kg, β-cyclocitral ≥55umol / L, effective viable bacteria count ≥200 million / mL, and pH value is 4.0.

[0050] The small molecule organic carbon nutrient solution involved in Examples 1-6 all adopts the above-mentioned small molecule organic carbon nutrient solution.

[0051] Example 1 Indoor potted plant test

[0052] This embodiment provides a group of indoor potted plant experiments, which are effect verification experiments comparing conventional fertilization by farmers and the small molecule organic carbon nutrient solution provided by the present invention and the screening of the optimal application amount thereof under the same irrigation conditions.

[0053] Test location: Sinochem Agriculture Linyi R&D Center, Linyi City, Shandong Province

[0054] Test variety: Xianyu 1225

[0055] Test time: November 10, 2022 - December 2, 2022

[0056] Experimental design: The potted soil was made from the corn planting field in Wuyuan County, Bayannur City, Inner Mongolia; the chemical properties of the soil were pH 8.86 and EC 955us / cm; it was cultured in an artificial climate room; it was treated before corn sowing and at the two-leaf-one-heart stage by irrigation; the germination rate was counted on the seventh day after sowing; the corn was harvested at the four-leaf-one-heart stage, and the fresh weight of the aboveground part of the corn and the pH and EC values ​​of the potted soil were measured.

[0057] The experiment set up 4 treatments, namely:

[0058] Treatment 1: blank control CK, according to the conventional fertilization method of local farmers, 15-15-15 compound fertilizer was applied as base fertilizer, the application amount was 40kg / mu, and it was evenly mixed with potting soil before sowing. The amount of water for irrigation before sowing and at the two-leaf and one-heart stage was the same as that of the treatment solution;

[0059] Treatment 2: T1, irrigate with low-molecular organic carbon nutrient solution before sowing and at the two-leaf and one-heart stage, with application rates of 2kg / mu and 5kg / mu respectively;

[0060] Treatment 3: T2, irrigate with low-molecular organic carbon nutrient solution before sowing and at the two-leaf and one-heart stage, with application rates of 5kg / mu and 10kg / mu respectively;

[0061] Treatment 4: T3, irrigate with low-molecule organic carbon nutrient solution before sowing and at the two-leaf and one-heart stage, with application rates of 8kg / mu and 15kg / mu respectively.

[0062] Note: The amount of fertilizer applied in the potted plant experiment was calculated by converting 150,000 kg of soil per mu of land into the actual soil mass in each pot.

[0063] The test results are shown in Table 1:

[0064] Table 1: Experimental results of different treatments

[0065] deal with Seedling rate (%) Fresh weight of aboveground parts (g / plant) Soil pH Soil EC (us / cm) CK 65.33 2.08 8.81 945.00 T1 75.00 2.62 8.40 851.67 T2 87.33 3.19 7.86 778.67 T3 70.00 2.41 8.36 836.67

[0066] The experiment showed that compared with the conventional fertilization method of farmers, the application of small molecule organic carbon nutrient solution during the corn seed germination period and the two-leaf and one-heart period can significantly increase the corn germination rate, increase the aboveground biomass, significantly improve the growth of the seedling stage, effectively improve the soil pH, and reduce soil salinity. Among them, the best application amount T2 treatment compared with the control CK treatment: the germination rate increased by 33.68%, the aboveground fresh weight increased by 53.21%, the soil pH decreased by 0.95, and the soil EC decreased by 166.33us / cm.

[0067] Example 2 Indoor Cell Test

[0068] This example provides a set of indoor plot tests, the chemical properties of the soil are pH 8.86, EC 955us / cm; the application amount of T2 treatment in Example 1 is used to conduct drip irrigation and farmers' conventional fertilization methods and planting patterns to compare the effect verification test at the seed germination stage and the two-leaf one-heart stage. The soil in this test plot is non-saline-alkali soil, and this test only verifies the growth-promoting effect of small molecule organic carbon nutrient solution under different planting patterns.

[0069] Test location: Sinochem Agriculture Linyi R&D Center, Linyi City, Shandong Province

[0070] Test variety: Xianyu 1225

[0071] Test time: December 15, 2022 - March 20, 2023

[0072] Experimental Design:

[0073] The experiment set up two treatments:

[0074] Treatment 1: blank control CK, according to the conventional fertilization method of local farmers, 15-15-15 compound fertilizer was applied as base fertilizer, the application rate was 40kg / mu, it was spread on the soil surface before sowing, and watered by flooding after furrowing and sowing;

[0075] Treatment 2: T4, using the planting method of ridge-forming film-covering and drip irrigation, drip irrigation of small molecule organic carbon nutrient solution after sowing and at the two-leaf and one-heart stage, with application rates of 5kg / mu and 10kg / mu respectively;

[0076] Each treatment area is 1 mu.

[0077] CK treatment: CK treatment adopted the planting method of flat ground furrow sowing and flood irrigation;

[0078] T4 treatment: First, ridges were built with a shoulder width of 40 cm, a height of 15 cm, and a spacing of 80 cm between ridges; single-row crops were planted with a sowing spacing of 20 cm; a single drip irrigation belt was arranged on each ridge, and the drip irrigation belt had a drip irrigation distance of 20 cm, and the drip irrigation position was adjacent to the sowing position; each ridge was covered with film, and drip irrigation was carried out after film covering, and the drip irrigation planting mode under film was adopted. Samples were collected at the four-leaf and one-heart stage of corn to measure the fresh weight of the aboveground part of corn; the plot yield was measured at the corn maturity stage.

[0079] Each treatment was repeated three times, and 15 plants were randomly selected from each repetition for determination of relevant indicators.

[0080] The test results are shown in Table 2:

[0081] Table 2: Experimental results of different treatments

[0082] deal with Fresh weight of aboveground parts (g / plant) Yield (kg / mu) CK 2.53 520.26 T4 3.39 580.40

[0083] The experiment showed that compared with farmers' conventional fertilization and planting patterns, the application of small molecule organic carbon nutrient solution during the germination period and the two-leaf-one-heart period of corn seeds can significantly increase the aboveground biomass of seedlings, improve the growth of seedlings and increase yields. Among them, the T4 treatment increased the aboveground fresh weight of seedlings by 33.82% and the yield by 11.56% compared with the control CK treatment.

[0084] Example 3 Field test verification

[0085] This embodiment provides a set of field tests, the chemical properties of the soil are pH 8.86, EC 955us / cm; referring to the T4 treatment in Example 2, drip irrigation was carried out at the corn seed germination stage and the two-leaf one-heart stage to compare the effect verification test with the conventional fertilization method and planting pattern of farmers.

[0086] Test location: Fuxing Village, Fuxing Town, Wuyuan County, Bayannur City, Inner Mongolia

[0087] Test variety: Xianyu 1225

[0088] Test time: June 10, 2023 - September 15, 2023

[0089] Experimental Design:

[0090] The experiment set up two treatments:

[0091] Treatment 1: blank control CK, according to the conventional fertilization method of local farmers, 15-15-15 compound fertilizer was applied as base fertilizer, the application rate was 40kg / mu, it was spread on the soil surface before sowing, and watered by flooding after furrowing and sowing;

[0092] Treatment 2: T5-1, using the planting method of ridge-forming film-covering and drip irrigation, drip irrigation of small molecule organic carbon nutrient solution after sowing and at the two-leaf and one-heart stage, with application rates of 5kg / mu and 10kg / mu respectively;

[0093] Each treatment area is 2 mu.

[0094] Test method: CK treatment adopted the planting method of flat ground furrow sowing and flood irrigation; T5-1 treatment adopted the planting method of ridge covering and drip irrigation: first ridge, ridge shoulder width 40cm, ridge height 15cm, ridge spacing 80cm; plant single row of crops, sowing spacing 20cm; arrange single drip irrigation belt on each ridge, drip irrigation belt dripper spacing 20cm, dripper position adjacent to sowing position; each ridge was covered with film, and drip irrigation was carried out after film covering, using the drip irrigation planting mode under film. Samples were collected at the four-leaf and one-heart stage of corn to measure the fresh weight of the aboveground part of corn; the yield and soil pH and EC values ​​were measured at the corn maturity stage.

[0095] Each treatment was repeated three times, and 15 plants were randomly selected from each repetition for determination of relevant indicators.

[0096] The test results are shown in Table 3:

[0097] Table 3: Experimental results of different treatments

[0098]

[0099] The experiment showed that compared with the conventional fertilization and planting patterns of farmers, the application of small molecule organic carbon nutrient solution during the corn seed germination period and the two-leaf one-heart period can significantly increase the corn germination rate, increase the aboveground biomass, significantly improve the growth of the seedling stage, effectively improve the soil pH, and reduce soil salinity. Compared with the control CK treatment, the T5-1 treatment increased the germination rate by 26.98%, the aboveground fresh weight by 29.62%, the yield by 13.15%, the soil pH by 0.93, and the soil EC by 160.00us / cm.

[0100] Example 4 Field test verification

[0101] This embodiment provides a set of field tests, the chemical properties of the soil are pH 8.9, EC 1145us / cm; referring to the T4 treatment in Example 2, drip irrigation was carried out at the corn seed germination stage and the two-leaf one-heart stage to compare the effect verification test with the conventional fertilization method and planting pattern of farmers.

[0102] Test location: Minli Village, Xingongzhong Town, Wuyuan County, Bayannur City, Inner Mongolia

[0103] Test variety: Xianyu 1225

[0104] Test time: June 5, 2023 - September 10, 2023

[0105] Experimental Design:

[0106] The experiment set up two treatments:

[0107] Treatment 1: blank control CK, according to the conventional fertilization method of local farmers, 15-15-15 compound fertilizer was applied as base fertilizer, the application rate was 40kg / mu, it was spread on the soil surface before sowing, and watered by flooding after furrowing and sowing;

[0108] Treatment 2: T5-2, using the planting method of ridge-forming film-covering and drip irrigation, drip irrigation of small molecule organic carbon nutrient solution after sowing and at the two-leaf and one-heart stage, with application rates of 5kg / mu and 10kg / mu respectively;

[0109] Each treatment area is 2 mu.

[0110] Test method: CK treatment adopted the planting method of flat ground furrow sowing and flood irrigation; T5-2 treatment adopted the planting method of ridge covering film drip irrigation: first ridge, ridge shoulder width 40cm, ridge height 15cm, ridge spacing 80cm; plant single row of crops, sowing spacing 20cm; arrange single drip irrigation belt on each ridge, drip irrigation belt dripper spacing 20cm, dripper position adjacent to sowing position; each ridge was covered with film, and drip irrigation was carried out after film covering, using the drip irrigation planting mode under film. Samples were collected at the four-leaf and one-heart stage of corn to measure the fresh weight of the aboveground part of corn; the yield and soil pH and EC values ​​were measured at the corn maturity stage.

[0111] Each treatment was repeated three times, and 15 plants were randomly selected from each repetition for determination of relevant indicators.

[0112] The test results are shown in Table 4:

[0113] Table 4: Experimental results of different treatments

[0114]

[0115]

[0116] The experiment showed that compared with the conventional fertilization and planting patterns of farmers, the application of small molecule organic carbon nutrient solution during the corn seed germination period and the two-leaf one-heart period can significantly increase the corn germination rate, increase the aboveground biomass, significantly improve the growth of the seedlings, effectively improve the soil pH, and reduce soil salinity. Compared with the control CK treatment, the T5-2 treatment increased the germination rate by 29.70%, the aboveground fresh weight by 31.62%, the yield by 14.28%, the soil pH by 1.02, and the soil EC by 154.33us / cm.

[0117] Example 5 Field test verification

[0118] This embodiment provides a set of field tests, the chemical properties of the soil are pH 9.0 and EC 1225 us / cm; referring to the T4 treatment in Example 2, drip irrigation was carried out at the corn seed germination stage and the two-leaf one-heart stage to compare the effect verification test with the conventional fertilization method and planting pattern of farmers.

[0119] Test location: Heping Village, Taohai Town, Wuyuan County, Bayannur City, Inner Mongolia

[0120] Test variety: Xianyu 1225

[0121] Test time: June 15, 2023 - September 15, 2023

[0122] Experimental Design:

[0123] The experiment set up two treatments:

[0124] Treatment 1: blank control CK, according to the conventional fertilization method of local farmers, 15-15-15 compound fertilizer was applied as base fertilizer, the application rate was 40kg / mu, it was spread on the soil surface before sowing, and watered by flooding after furrowing and sowing;

[0125] Treatment 2: T5-3, using the planting method of ridge-forming film-covering and drip irrigation, drip irrigation of small molecule organic carbon nutrient solution after sowing and at the two-leaf and one-heart stage, with application rates of 5kg / mu and 10kg / mu respectively;

[0126] Each treatment area is 2 mu.

[0127] Test method: CK treatment adopted the planting method of flat ground furrow sowing and flood irrigation; T5-3 treatment adopted the planting method of ridge film covering and drip irrigation: first ridge, ridge shoulder width 40cm, ridge height 15cm, ridge spacing 80cm; plant single row of crops, sowing spacing 20cm; arrange single drip irrigation belt on each ridge, drip irrigation belt dripper spacing 20cm, dripper position adjacent to sowing position; each ridge was covered with film, and drip irrigation was carried out after film covering, using the drip irrigation planting mode under film. Samples were collected at the four-leaf and one-heart stage of corn to measure the fresh weight of the aboveground part of corn; the yield and soil pH and EC values ​​were measured at the corn maturity stage.

[0128] Each treatment was repeated three times, and 15 plants were randomly selected from each repetition for determination of relevant indicators.

[0129] The test results are shown in Table 5:

[0130] Table 5: Experimental results of different treatments

[0131]

[0132] The experiment showed that compared with the conventional fertilization and planting patterns of farmers, the application of small molecule organic carbon nutrient solution during the corn seed germination period and the two-leaf one-heart period can significantly increase the corn germination rate, increase the aboveground biomass, significantly improve the growth of the seedling stage, effectively improve the soil pH, and reduce soil salinity. Compared with the control CK treatment, the T5-3 treatment increased the germination rate by 25.42%, the aboveground fresh weight by 26.29%, the yield by 10.10%, the soil pH by 0.97, and the soil EC by 178.33us / cm.

[0133] Example 6 Field Test Verification

[0134] This embodiment provides a set of field tests, the chemical properties of the soil are pH 9.0 and EC 880us / cm; referring to the T4 treatment in Example 2, drip irrigation was carried out at the corn seed germination stage and the two-leaf one-heart stage to compare the effect verification test with the conventional fertilization method and planting pattern of farmers.

[0135] Test location: Xianjin Village, Taohai Town, Wuyuan County, Bayannur City, Inner Mongolia

[0136] Test variety: Xianyu 1225

[0137] Test time: June 18, 2023 - September 20, 2023

[0138] Experimental Design:

[0139] The experiment set up two treatments:

[0140] Treatment 1: blank control CK, according to the conventional fertilization method of local farmers, 15-15-15 compound fertilizer was applied as base fertilizer, the application rate was 40kg / mu, it was spread on the soil surface before sowing, and watered by flooding after furrowing and sowing;

[0141] Treatment 2: T5-4, using the planting method of ridge-forming film-covering and drip irrigation, drip irrigation of small molecule organic carbon nutrient solution after sowing and at the two-leaf and one-heart stage, with application rates of 5kg / mu and 10kg / mu respectively;

[0142] Each treatment area is 2 mu.

[0143] Test method: CK treatment adopted the planting method of flat ground furrow sowing and flood irrigation; T5-4 treatment adopted the planting method of ridge covering film drip irrigation: first ridge, ridge shoulder width 40cm, ridge height 15cm, ridge spacing 80cm; plant single row of crops, sowing spacing 20cm; arrange single drip irrigation belt on each ridge, drip irrigation belt dripper spacing 20cm, dripper position adjacent to sowing position; each ridge was covered with film, and drip irrigation was carried out after film covering, using the drip irrigation planting mode under film. Samples were collected at the four-leaf and one-heart stage of corn to measure the fresh weight of the aboveground part of corn; the yield and soil pH and EC values ​​were measured at the corn maturity stage.

[0144] Each treatment was repeated three times, and 15 plants were randomly selected from each repetition for determination of relevant indicators.

[0145] The test results are shown in Table 6:

[0146] Table 6: Experimental results of different treatments

[0147]

[0148] The experiment showed that compared with the conventional fertilization and planting patterns of farmers, the application of small molecule organic carbon nutrient solution during the corn seed germination period and the two-leaf one-heart period can significantly increase the corn germination rate, increase the aboveground biomass, significantly improve the growth of the seedling stage, effectively improve the soil pH, and reduce soil salinity. Compared with the control CK treatment, the T5-4 treatment increased the germination rate by 27.18%, the aboveground fresh weight by 30.38%, the yield by 11.89%, the soil pH by 0.95, and the soil EC by 164.33us / cm.

[0149] The test results of different treatments and controls of Examples 1-6 are shown in Table 7:

[0150] Table 7: Experimental results of different treatments

[0151]

[0152] As can be seen from the data in the above table, it can be seen from Example 1 and Example 2 that the small molecule organic carbon nutrient solution provided by the present invention is not only suitable for saline-alkali land, but also has good fertilizer efficiency in non-saline-alkali land, can significantly improve the emergence rate and seedling growth of corn, effectively improve the pH of the soil, reduce the EC (electrical conductivity) value, significantly increase corn yield, and the application amount is small, which greatly reduces the work intensity of the application process. It can be seen from Example 2-Example 5 that the small molecule organic carbon nutrient solution provided by the present invention is combined with ridge film covering drip irrigation, and the planting mode applied during the corn seed germination period and the two-leaf one-heart period is suitable for a variety of soil conditions and planting environments, which can not only significantly improve the emergence rate of corn, increase the biomass of the aboveground part, and significantly improve the growth of the seedling stage, but also effectively improve the pH of the soil, reduce soil salinity, and optimize the soil environment on the premise of increasing yield, making a favorable contribution to expanding the cultivated area of ​​farmland.

[0153] Example 7

[0154] The raw materials of the small molecule organic carbon nutrient solution are composed of the following components by weight: 80 parts of livestock and poultry liquid manure, 15 parts of organic auxiliary materials, and 5 parts of core ingredients. The auxiliary material is brown sugar, and the weight ratio of acid-producing fermentation bacteria, functional bacterial agents, biological enzymes, free amino acids, seaweed extracts, β-cyclocitral, and trace elements in the core ingredients is 10:10:10:2:2:2:1. The nutrient content of the small molecule organic carbon nutrient solution is: organic matter 140g / L, humic acid 20g / L, macroelements 70g / L, trace elements 25g / L, protease activity ≥550u / mL, cellulase activity ≥35U / mL, crude protein ≥20g / 100g, soluble sugar ≥9.5g / L, free amino acids ≥16.5%, indole-3-acetic acid ≥5000ug / kg, gibberellin ≥20ug / kg, β-cyclocitral ≥55umol / L, effective viable bacteria count ≥200 million / mL, and pH value is 3.8.

[0155] Example 8

[0156] The raw materials of the small molecule organic carbon nutrient solution are composed of the following components by weight: 60 parts of livestock and poultry liquid manure, 30 parts of organic auxiliary materials, and 10 parts of core ingredients. The auxiliary material is honey, and the weight ratio of acid-producing fermentation bacteria, functional bacterial agents, biological enzymes, free amino acids, seaweed extracts, β-cyclocitral, and trace elements in the core ingredients is 20:20:20:10:10:10:5. The nutrient content of the small molecule organic carbon nutrient solution is: organic matter 140g / L, humic acid 20g / L, macroelements 70g / L, trace elements 25g / L, protease activity ≥550u / mL, cellulase activity ≥35U / mL, crude protein ≥20g / 100g, soluble sugar ≥9.5g / L, free amino acids ≥16.5%, indole-3-acetic acid ≥5000ug / kg, gibberellin ≥20ug / kg, β-cyclocitral ≥55umol / L, effective viable bacteria count ≥200 million / mL, and pH value is 4.6.

[0157] The small molecule organic carbon nutrient solution in Examples 7 and 8 was combined with the ridging film covering drip irrigation technology for corn planting, and the effect was consistent with that of Examples 1-6, which will not be repeated here.

[0158] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A small molecule organic carbon nutrient solution, characterized in that: Its raw materials are composed of the following components by weight: 60-80 parts of liquid livestock and poultry manure; 10-30 parts of organic excipients; 5-10 servings of core ingredients; The core components are composed of acid-producing fermentation bacteria, functional bacterial agents, biological enzymes, free amino acids, seaweed extracts, beta-cyclocitral, and trace elements.

2. The small molecule organic carbon nutrient solution according to claim 1, characterized in that: The organic auxiliary materials include honey and / or brown sugar; The acid-producing fermentation bacteria include one or a combination of acetobacillus, yeast, and lactic acid bacteria; And / or, the functional bacterial agent is a microbial agent with the functions of solubilizing phosphorus, solubilizing potassium, fixing nitrogen and promoting decay, including one or more combinations of Bacillus, Streptomyces jingyangensis, mycorrhizal fungi and brown nitrogen-fixing bacteria, and the Bacillus includes one or more combinations of Bacillus licheniformis, Bacillus megaterium, Bacillus colloids, Bacillus gelatinous, Bacillus subtilis and Bacillus thuringiensis; And / or, the biological enzyme includes one or a combination of cellulase, protease, superoxide dismutase, and hydrolase; And / or, the free amino acids include one or a combination of glycine, glutamic acid, arginine, leucine, proline, γ-aminobutyric acid, and β-alanine; And / or, the seaweed extract comprises one or a combination of seaweed polysaccharide, alginic acid, betaine, sterol, and mannitol; And / or, the trace elements include one or a combination of Ca, Mg, B, Fe, Mn, Cu, and Zn.

3. The small molecule organic carbon nutrient solution according to claim 2, characterized in that: The nutrient content in the small molecule organic carbon nutrient solution is: Organic matter 140g / L, humic acid 20g / L, macroelements 70g / L, trace elements 25g / L, protease activity ≥550U / mL, cellulase activity ≥35U / mL, crude protein ≥20g / 100g, soluble sugar ≥9.5g / L, free amino acids ≥16.5%, indole-3-acetic acid ≥5000ug / kg, gibberellin ≥20ug / kg, β-cyclocitral ≥55umol / L, effective viable count ≥200 million / mL; The pH range of the organic carbon nutrient solution is 3.8-4.6; Preferably, the major elements include N, P2O5, and K2O.

4. A method for preparing a small molecule organic carbon nutrient solution, characterized in that: It is obtained by fermenting livestock and poultry liquid manure as the main body, adding organic auxiliary materials and core ingredients required for aerobic fermentation.

5. The preparation method according to claim 4, characterized in that: include: First, add the liquid livestock and poultry manure after solid-liquid separation into a fermentation device, add half of the core components in proportion, and turn on the aeration device for internal circulation to ensure that the materials are fully mixed and continue for 5-7 days, and control the pH value in the range of 3.5-5.5 to obtain a mixed liquid; Then, organic auxiliary materials and the other half of the core ingredients are added to the mixed solution, fermented for 5-7 days, and the pH value is controlled to 4.5 to form a small molecule organic carbon nutrient solution.

6. Use of the small molecule organic carbon nutrient solution according to any one of claims 1 to 3 or the small molecule organic carbon nutrient solution prepared by the preparation method according to claim 4 or 5 in reducing soil pH and EC value.

7. Use of the small molecule organic carbon nutrient solution according to any one of claims 1 to 3 or the small molecule organic carbon nutrient solution prepared by the preparation method according to claim 4 or 5 in the cultivation of crops in saline-alkali land.

8. Use of the small molecule organic carbon nutrient solution according to any one of claims 1 to 3 or the small molecule organic carbon nutrient solution prepared by the preparation method according to claim 4 or 5 in saline-alkali land to improve the salt tolerance, stress resistance, and yield of crops.

9. A method for planting salt-inhibiting, stress-resistant and seedling-raising plants, characterized in that: The invention comprises adopting ridge-forming film-covering drip irrigation technology to drip-irrigate the small molecule organic carbon nutrient solution described in any one of claims 1 to 3 or the small molecule organic carbon nutrient solution prepared by the preparation method described in claim 4 or 5 during the seed germination period and the two-leaf one-heart period in the plant planting process.

10. The planting method according to claim 9, characterized in that: The amount of the small molecule organic carbon nutrient solution used for drip irrigation during the seed germination period is 2-7kg / mu, preferably 5kg / mu; the amount of the small molecule organic carbon nutrient solution used for drip irrigation during the two-leaf and one-heart period is 5-15kg / mu, preferably 10kg / mu. When used, it is dripped in with water. Generally, the amount of water used per mu is 20-30m 3 / mu.

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