A fully automated DNA purification and amplification device for solid samples

Abstract

The application discloses a kind of solid sample full automation DNA purification and amplification device, including workbench, and sample sleeve plate, centrifugal module, film sealing module, puncture module, heating oscillation module, plate moving module and mechanical arm being set on workbench, sample sleeve plate includes sample plate and receiving plate, receiving plate is set below sample plate and the side edge of two is clamped as a whole, sample plate is equipped with sample hole, receiving plate is equipped with receiving hole, sample hole is set in receiving hole and the side wall of two close to liquid outlet is interference fit.Centrifugal module is used to separate sample plate and receiving plate under the action of centrifugation after lysis;Film sealing module is used to attach adhesive film on the sample hole of sample sleeve plate;Puncture module is used to puncture the adhesive film on the sample sleeve plate.The sample plate and receiving plate of the sample sleeve plate are automatically separated by the centrifugal module, the sample sleeve plate does not leak under the condition of film heating, ensures that lysis system is stable, oscillation is mixed thoroughly, and evidence is fully soaked.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of biological equipment, and particularly relates to a full-automatic DNA purification and amplification device for solid samples. BACKGROUND

[0002] Solid samples (such as solid samples and tissues such as fecal swabs, exfoliated cell sticks, and environmental sampling swabs) in laboratories such as judicature, clinics, dynamic inspection, and disease control need to extract and purify macromolecular target substances such as proteins and nucleic acids, must be lysed to release, collect necessary sample cell and tissue lysis contents, and then can maximize the collection of sample leachate through centrifugation, and then perform subsequent DNA purification and reaction system construction processes.

[0003] At present, the mainstream automatic processing solid sample lysis and recovery product technology is to directly suck the lysis product from a large container, and due to a large amount of leachate residue, some solid carriers themselves absorb a large amount of liquid, which is not suitable for the processing of trace samples. In order to completely obtain all the leachate, centrifugation operation is needed, and manual centrifugation sleeve operation is more common at present. Since the exfoliated cells and tissues of forensic DNA samples are basically dry samples, high-temperature incubation is needed in the lysis process, and sealing is needed to prevent liquid evaporation and pollution, and the centrifugal filter tube containing the sample is embedded into a larger centrifugal tube. After lysis and centrifugation, the centrifugal filter tube containing the sample carrier and the outer tube containing the centrifugal recovery product need to be separated, so that the lysis product can be taken out.

[0004] At present, there is no device to realize automatic sealing, and due to the characteristics of the sample container being through from top to bottom, the top cannot be sealed, the bottom is closed, and the centrifugal liquid recovery is also not supported. Since the sample plate cannot be sealed during lysis (because sealing will cause swelling and liquid leakage), the unsealed lysis sample plate is easy to cause liquid evaporation during heating, which not only affects the sample lysis effect, but also causes corrosion or pollution to the equipment interior, which does not meet the specification requirements (ENFSI:FSR-G-208:6.2.7) of sample processing in the laboratory.

[0005] Therefore, a full-automatic DNA purification and amplification device for solid samples is urgently needed. SUMMARY

[0006] In order to solve the defects in the prior art, the application provides a full-automatic DNA purification and amplification device for solid samples.

[0007] In order to solve the above technical problems, the application provides the following technical solutions:

[0008] The application provides a full-automatic DNA purification and amplification device for solid samples, comprising:

[0009] A workbench, a purification plate site and a trace reagent site are arranged on the workbench; and a centrifugal module is arranged on the workbench:

[0010] A sample sleeve plate comprises a sample plate and a receiving plate, the receiving plate is arranged below the sample plate and the two side edges are clamped together, the sample plate is provided with a sample hole, the receiving plate is provided with a receiving hole, the sample hole is sleeved in the receiving hole, and the side walls close to the liquid outlet of the sample hole and the receiving hole are in interference fit;

[0011] A centrifugal module is used to separate the sample plate and the receiving plate under the action of centrifugation after lysis;

[0012] A film sealing module is used to attach a sealing film on the sample hole of the sample sleeve plate;

[0013] A puncture module is used to pierce the sealing film on the sample sleeve plate;

[0014] A heating and oscillation module is used to heat and oscillate the sample sleeve plate to realize lysis reaction;

[0015] A plate moving module is used to move the sample sleeve plate according to the setting;

[0016] A mechanical arm is used to drive the plate moving module and the puncture module according to the setting.

[0017] Preferably, at least one pair of symmetrical side edges of the sample plate and the receiving plate are clamped together by a mortise and tenon structure.

[0018] Preferably, the side wall close to the liquid outlet of the sample hole is sequentially provided with a first sample transition section, a sample filter screening section, a second sample transition section and a sample liquid outlet section from top to bottom, the side wall close to the liquid outlet of the receiving hole is sequentially provided with a first receiving transition section, a receiving filter screening section, a second receiving transition section and a receiving liquid outlet section from top to bottom, the first receiving transition section, the receiving filter screening section, the second receiving transition section and the receiving liquid outlet section are arranged one by one on the outside of the first sample transition section, the sample filter screening section, the second sample transition section and the sample liquid outlet section, and the sample liquid outlet section and the receiving liquid outlet section are in interference fit.

[0019] The centrifugal module comprises:

[0020] A cabin body, which is a cylindrical structure, is provided with a movable cabin door on the top cover;

[0021] A basket comprises a first basket body and a second basket body, the first basket body and the second basket body are arranged on a horizontal rotor, and the first basket body and the second basket body are provided with a sample sleeve plate positioner and a balancing plate positioner for placing a sample sleeve plate and a balancing plate, respectively;

[0022] A horizontal rotor is arranged on the bottom plate of the cabin body and rotates to drive the hanging basket to rotate around the center thereof;

[0023] A rotary drive motor is in transmission connection with the horizontal rotor and rotates to drive the horizontal rotor to rotate around the center thereof;

[0024] A cabin door drive motor is in transmission connection with the cabin door and is used to open or close the cabin door;

[0025] A photoelectric sensor is arranged on the inner wall of the cabin body and is used to sense the arrival of the first hanging basket body or the second hanging basket body at the entrance and exit position of the cabin body.

[0026] Preferably, the liquid treatment module is used to inject liquid into the balancing plate, and the balancing plate is used to balance the centrifugal module.

[0027] Preferably, the sample adding tip is loaded by the multi-channel pipetting module and is used to transfer the lysis product; and the multi-channel pipetting module is driven by the mechanical arm.

[0028] Preferably, the DNA storage plate and the PCR plate are moved to the film sealing module by the plate moving module according to the setting.

[0029] Preferably, the waste channel is used to load waste.

[0030] Preferably, the cover opening module is used to open and close the test tube cover.

[0031] Preferably, the barcode instrument is used to scan the barcodes on the sample sleeve plate and the sample adding tip and record the consumable information.

[0032] Compared with the prior art, the present application has the following beneficial effects:

[0033] (1) The present application uses the centrifugal module to automatically separate the sample plate and the receiving plate of the sample sleeve plate, and the sample sleeve plate will not leak liquid in the film heating state, ensuring the stability of the lysis system, complete shock mixing and sufficient sample immersion.

[0034] (2) The present application stores the DNA storage plate film sealing at low temperature, reduces the evaporation loss of trace reagents, and ensures the stability of the template system.

[0035] (3) The present application keeps the low-temperature state during the amplification system construction process, ensures the stability of the reaction system, and directly seals the film after the system construction is completed, ensuring the reliability of the reaction system.

[0036] (4) The functional module of the mechanical arm can be automatically replaced according to needs, and the multi-combination type sample adding channel not only meets the rapid distribution of large-volume liquid, but also guarantees the accuracy of small-volume liquid treatment, so that the liquid treatment precision is higher and the range is wider, and each functional module can be realized by cooperating with a liquid treatment module, a plate moving module, a puncture module, a cap opening module, a bar code instrument and the like. BRIEF DESCRIPTION OF DRAWINGS

[0037] Figure 1 is the overall structure schematic diagram of a solid sample full-automatic DNA purification and amplification device of the present application;

[0038] Figure 2 is the structure schematic diagram of the state of the sample sleeve plate not separated in the present application;

[0039] Figure 3 is the structure schematic diagram of the mortise and tenon structure in the present application;

[0040] Figure 4 is the structure schematic diagram of the state of the sample sleeve plate after separation in the present application;

[0041] Figure 5 is the structure schematic diagram of the sample hole and the receiving hole after the sample sleeve plate is separated in the present application;

[0042] Figure 6 is the structure schematic diagram of the sample hole and the receiving hole when the sample sleeve plate is not separated in the present application;

[0043] Figure 7 is the structure schematic diagram of the sample hole in the present application;

[0044] Figure 8 is the structure sectional view of the sample hole and the receiving hole when the sample sleeve plate is not separated in the present application;

[0045] Figure 9 is the structure schematic diagram of the magnetic rod sleeve and the reagent plate in the present application;

[0046] Figure 10 is the structure schematic diagram of the centrifugal module in the present application;

[0047] Figure 11 is the structure side view of the centrifugal module in the present application. DETAILED DESCRIPTION

[0048] The preferred embodiments of the present application are described below in conjunction with the accompanying drawings, and it should be understood that the preferred embodiments described herein are only used to illustrate and explain the present application, and are not used to limit the present application.

[0049] In the description of the present application, it needs to be understood that the terms "left", "right" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings of the specification, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application.

[0050] In the description of the present application, it needs to be understood that unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connection" should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integrally connected; it can be directly connected, or indirectly connected through an intermediate medium. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0051] As shown in Figures 1 to 11 The embodiment provides a solid sample full-automatic DNA purification and amplification device, which comprises a workbench and a sample sleeve plate, a centrifugal module 3-2, a film sealing module 3-1, a puncture module 3-3, a heating and oscillation module 3-8, a plate moving module 3-7 and a mechanical arm 3-18 arranged on the workbench. The workbench is provided with a purification plate site 3-4 and a micro-reagent site 3-10.

[0052] As shown in Figures 2 to 8 The sample sleeve plate comprises a sample plate 1-1 and a receiving plate 1-2, the receiving plate 1-2 is arranged below the sample plate 1-1, the front and rear sides of the sample plate 1-1 and the receiving plate 1-2 are respectively connected as a whole through two mortise and tenon structures 1-4, and the left and right sides of the sample plate 1-1 and the receiving plate 1-2 are respectively connected as a whole through two mortise and tenon structures 1-4. The mortise and tenon structure can not only ensure that the sample plate 1-1 and the receiving plate 1-2 are closely combined, but also can realize automatic separation of the sample plate 1-1 and the receiving plate 1-2 under the action of centrifugal force without the aid of other tools.

[0053] As shown in Figure 6 and Figure 7As shown, the sample plate 1-1 is provided with 24 sample holes, the receiving plate 1-2 is provided with 24 receiving holes, and the sample hole 1-6 is sleeved in the receiving hole 1-7. The side wall of the sample hole 1-6 close to the liquid outlet is sequentially provided with a first sample transition section, a sample filter screening section, a second sample transition section and a sample liquid outlet section 1-5 from top to bottom, the side wall of the receiving hole 1-7 close to the liquid outlet is sequentially provided with a first receiving transition section, a receiving filter screening section, a second receiving transition section and a receiving liquid outlet section, the first receiving transition section, the receiving filter screening section, the second receiving transition section and the receiving liquid outlet section are arranged outside the first sample transition section, the sample filter screening section, the second sample transition section and the sample liquid outlet section 1-5 one by one, the sample liquid outlet section 1-5 and the receiving liquid outlet section are interference fit, and the sample filter screening section is filled with a sieve plate 1-3. The diameters of the first sample transition section, the second sample transition section, the sample liquid outlet section 1-5, the first receiving transition section, the second receiving transition section and the receiving liquid outlet section are gradually reduced from top to bottom, the design of the transition section facilitates the fixation of the sieve plate, and the interference fit structure can realize the sealing effect and automatically separate under the action of centrifugal force.

[0054] As shown in Figure 10 and Figure 11 As shown, the centrifugal module 3-2 includes a cabin body 5-3, a hanging basket 5-1, a horizontal rotor 5-2, a rotation driving motor, a cabin door driving motor and a photoelectric sensor. The cabin body 5-3 is a cylindrical structure, and the top cover of the cabin body 5-3 is provided with a movable cabin door. The hanging basket 5-1 includes a first hanging basket body and a second hanging basket body, which are arranged on the horizontal rotor 5-2, and are provided with a sample sleeve plate positioner and a balancing plate positioner for placing a sample sleeve plate and a balancing plate, respectively. The horizontal rotor 5-2 is rotationally arranged on the bottom plate of the cabin body and is used to drive the hanging basket 5-1 to rotate around the center thereof. The output shaft of the rotation driving motor is in transmission connection with the horizontal rotor 5-2, and is used to drive the horizontal rotor to rotate around the center thereof. The output shaft of the cabin door driving motor is in transmission connection with the cabin door, and is used to open or close the cabin door. The photoelectric sensor is arranged on the inner wall of the cabin body, and is used to sense the arrival of the first hanging basket body or the second hanging basket body at the inlet and outlet positions of the cabin body. The centrifugal module 3-2 is used to separate the sample plate 1-1 and the receiving plate 1-2 under the action of centrifugal force after lysis. In this embodiment, the rotation driving motor can adopt any existing structure that can drive the horizontal rotor to rotate, and the cabin door driving motor can adopt any existing structure that can open or close the cabin door.

[0055] When centrifugation is needed, the rotation driving motor starts to rotate at low speed, when the first hanging basket body turns to the set position, the photoelectric sensor receives the photoelectric signal, and the rotation driving motor is locked. At this time, the cabin door driving motor drives the cabin door to open, and the sample sleeve plate is placed on the first hanging basket body. When the sample sleeve plate is successfully placed, the cabin door driving motor drives the cabin door to close, and the rotation driving motor rotates again at low speed; when the second hanging basket body moves to the set position, the photoelectric sensor receives the photoelectric signal, and the rotation driving motor is locked. At this time, the cabin door driving motor drives the cabin door to open again, and the balancing plate is put into the second hanging basket body. When the balancing plate is successfully placed, the cabin door driving motor drives the cabin door to close, and the rotation driving motor starts to operate according to the set centrifugation time and speed. When the centrifugation is finished, the cabin door is opened, and the sample plate 1-1 and the receiving plate 1-2 are taken out respectively.

[0056] In this embodiment, it also includes a film sealing module 3-1, a puncture module 3-3, a heating and oscillation module 3-8, a mechanical arm 3-18, a liquid treatment module 3-6, a balancing plate 3-14, a sample adding tip 3-13, a multi-channel pipetting module 3-9, a DNA storage plate 3-12, a PCR plate 3-11, a waste channel 3-17, a cap opening module 3-16 and a barcode instrument 3-15. The film sealing module 3-1 is used to attach a plastic sealing film on the sample holes of the sample sleeve plate. The puncture module 3-3 is used to pierce the plastic sealing film on the sample sleeve plate. The heating and oscillation module 3-8 is used to heat and oscillate the sample sleeve plate to achieve lysis reaction. The plate moving module 3-7 is used to move the sample sleeve plate according to the setting. The mechanical arm 3-18 is used to drive the plate moving module 3-7 and the puncture module 3-3 according to the setting. The liquid treatment module 3-6 is used to inject liquid into the balancing plate 3-14, and the balancing plate 3-14 is used to balance the centrifugation module 3-2. The sample adding tip 3-13 is loaded by the multi-channel pipetting module 3-9 and is used to transfer the lysis product; the multi-channel pipetting module 3-9 is driven by the mechanical arm 3-18. The plate moving module 3-7 moves the DNA storage plate 3-12 and the PCR plate 3-11 to the film sealing module 3-1 according to the setting. The waste channel 3-17 is used to load waste. The cap opening module 3-16 is used to open and close the test tube cap. The barcode instrument 3-15 is used to scan the barcodes on the sample sleeve plate and the sample adding tip 3-13 to record the consumable information.

[0057] In this embodiment, the cap opening module can automatically open and close the reagent tube cap, and can repeatedly add liquid and reseal the film on the sample processing plate in cooperation with the puncture module, thereby improving the processing capacity of complex samples.

[0058] In this embodiment, the PCR plate is added, and the sample concentration can be uniformly processed according to the fluorescence quantitative result. The DNA content of the low-concentration sample is improved through pre-expansion technology, and the detection success rate of trace samples is improved.

[0059] According to the comparison experiment data of the high-temperature pyrolysis process with and without sealing film, it is shown that the evaporation amount of liquid in the open sample plate is 3.2 times that in the sample plate with sealing film. The evaporated liquid not only leads to the instability of the pyrolysis system and the poor pyrolysis effect of the sample, but also long-term accumulation of the crystallized liquid in the working chamber, which increases the risk of aerosol pollution and corrosion of equipment parts in the working chamber.

[0060] In addition, according to the test data of the DNA template stored with and without sealing film at room temperature, it is shown that the DNA template without sealing film will evaporate 1-2 microliters per hour in the laboratory environment, which is very unfavorable for the trace sample with a small amount of obtained DNA solution. Therefore, it is a powerful measure to ensure the test result that the extracted template DNA is stored temporarily with sealing film as soon as possible to avoid unnecessary volatilization loss.

[0061] The working principle of the embodiment will be further described below.

[0062] The conventional magnetic / silica bead extraction reagent and consumables are pre-packaged in a 48-well plate (injection molding product) matched with the purifier (8 servings / plate). The pre-filled reagents include lysis solution, binding solution, washing solution and elution solution, magnetic / silica beads, etc. As shown in FIG. 2, before the magnetic bead extraction work starts, the magnetic rod sleeve 2-2 for transferring magnetic beads can be placed in the well of the reagent plate 2-1, and the well can keep the magnetic rod sleeve upright to facilitate the uploading of the magnetic rod sleeve. Figure 9

[0063] Preparation: The operator places the sample to be extracted in the sample sleeve plate, and places the pre-packaged reagent plate 2-1 and magnetic rod sleeve 2-2 on the purification plate site 3-4, and places the sample adding tip 3-13 and trace reagent on the designated position.

[0064] The following are automatic operations: the mechanical arm 3-18 automatically loads the plate transfer module 3-7, grabs the sample sleeve plate from the heating and shaking module 3-8, and scans the barcode with the barcode instrument 3-15, and records and confirms the barcode information of the consumables.

[0065] The mechanical arm 3-18 automatically loads the liquid treatment module 3-6, uploads the sample adding tip 3-13, and respectively takes the lysis solution from the pre-packaged purification plate site 3-4 and the protease PK from the trace reagent to add them into the sample sleeve plate.

[0066] The mechanical arm 3-18 automatically loads the plate transfer module 3-7, transfers the sample sleeve plate to the sealing film module 3-17, automatically seals the film, and then transfers the sample sleeve plate with the sealing film to the heating and shaking module 3-8 to start heating and pyrolysis, during which the sample cells are broken by shaking several times.

[0067] ​After lysis, the sample plate is transferred to the centrifugation module 3-2 by the plate transfer module 3-7, and the balance plate 3-14 is injected with a liquid balance according to the number of samples using the liquid treatment module 3-6. The balance plate is transferred to the centrifugation module 3-2, and the centrifugation is started.

[0068] Under the action of centrifugal force, the combined mortise and tenon structure 1-4 of the receiving plate 1-2 at the lower part and the sample plate 1-1 at the upper part of the sample plate will automatically disengage, and the bottom interference fit will naturally disappear. The lysate in the sample plate 1-1 flows into the receiving plate under the action of centrifugal force.

[0069] After centrifugation is completed, the centrifugation module opens the hatch, and the plate transfer module 3-7 first removes the sample plate, then removes the receiving plate, and places them back on the heating and shaking module 3-8 which has stopped heating.

[0070] The robotic arm 3-18 automatically loads the multi-channel pipetting module 3-9, loads the sample loading tip 3-13, and transfers the sample lysate recovered by centrifugation to the binding reagent hole site of the purification plate site 3-4, and discards the disposable pipette tip in the waste channel 3-21.

[0071] The magnetic rod 3-5 of the purification module loads the magnetic rod sleeve 2-2, and the magnetic beads are adsorbed and transferred to the binding hole site for rotational mixing, and the sample DNA is bound to the magnetic beads. The magnetic rod 3-5 is inserted into the magnetic rod sleeve 2-2, and after the magnetic beads are sucked, the magnetic rod sleeve 2-2 is sequentially transferred to the washing liquid 1, 2, and 3 hole sites for washing. After washing three times, the magnetic rod sleeve 2-2 transfers the magnetic beads to the elution liquid hole site, at which time the elution liquid corresponding to the hole site at the bottom of the elution hole site is heated to heat the elution liquid for DNA elution. After the DNA is eluted, the magnetic rod sleeve 2-2 adsorbs and transfers the magnetic beads to the first column of empty lysis liquid hole sites, and the magnetic beads that have completed the extraction task are left in the first column of hole sites. The purification instrument tray carrier is automatically removed, and the DNA extraction and purification steps are completed.

[0072] The multi-channel pipetting module 3-9 loads the sample loading tip 3-13, and transfers the DNA elution liquid from the purification plate site to the DNA storage plate 3-12. After transfer, the sample loading tip is discarded into the waste channel 3-21.

[0073] The liquid treatment module 3-6 sucks the prepared amplification reagent at the micro-reagent site 3-10, and distributes it to the PCR plate 3-11 of the reaction system construction, and sucks a certain amount of template from the DNA storage plate 3-12 and adds it to the reaction system solution of the PCR plate 3-11.

[0074] The plate transfer module 3-7 grabs the DNA storage plate 3-12 and the PCR plate 3-11 respectively for film sealing and preservation.

[0075] After the device completes the above work, the experimental report and sample information location file are output. When other samples are directly sealed and lysed by the recycling plate, the puncture module 3-3 can puncture the puncturable sealing film, and then the sample is sucked by the sample adding needle for processing. The trace reagent can be automatically opened by the cover opening module 3-16, and the test tube cover is automatically unscrewed. After the liquid is sucked, the cover is automatically screwed, so that the reagent is stable. The functional module can automatically change according to the needs of the method process.

[0076] Finally, it should be noted that the above only describes the preferred embodiments of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent replacements to some technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A fully automated DNA purification and amplification device for solid samples, characterized in that: include: A workbench, wherein the workbench is provided with purification plate positions (3-4) and trace reagent positions (3-10); And set on the workbench: The sample sleeve comprises a sample plate (1-1) and a receiving plate (1-2), wherein the receiving plate (1-2) is arranged below the sample plate (1-1) and the sides of the two are snap-fitted into one, the sample plate (1-1) is provided with a sample hole (1-6), the receiving plate (1-2) is provided with a receiving hole (1-7), the sample hole (1-6) is sleeved in the receiving hole (1-7), and the side walls of the two near the liquid outlet are interference fit; the side walls of the sample hole (1-6) near the liquid outlet are provided with a first sample transition section, a sample filtering section, a second sample transition section, a sample filtering section, a second sample transition section, a sample filtering section, a second sample transition section, a sample filtering section, a second sample transition section, a second sample transition section, a sample filtering section, a second sample transition section, a second sample transition section, a second sample transition section, a sample filtering section, a second sample transition section, a second sample transition section, a first sample transition section, a sample filtering section, a second sample transition ... The side wall of the receiving hole (1-7) near the liquid outlet is provided with a first receiving transition section, a receiving filtering and screening section, a second receiving transition section and a receiving liquid outlet section in sequence from top to bottom. The first receiving transition section, the receiving filtering and screening section, the second receiving transition section and the receiving liquid outlet section are arranged one by one on the outside of the first sample transition section, the sample filtering and screening section, the second sample transition section and the sample liquid outlet section (1-5). The sample liquid outlet section (1-5) and the receiving liquid outlet section are interference fit. The sample filtering and screening section is filled with a sieve plate (1-3); a centrifugal module (3-2), used for separating the sample plate (1-1) and the receiving plate (1-2) by centrifugation after lysis; A sealing module (3-1) is used for attaching a sealing film to the sample wells of the sample plate; The puncture module (3-3) is used to puncture the sealing film on the sample plate; Heating and shaking module (3-8), used to heat and shake the sample plate to achieve lysis reaction; The plate moving module (3-7) is used to move the sample plate according to the setting; The robotic arm (3-18) is used to drive the plate moving module (3-7) and the puncture module (3-3) according to settings.

2. The fully automated DNA purification and amplification device for solid samples according to claim 1, characterized in that: At least one set of two symmetrical side edges of the sample plate (1-1) and the receiving plate (1-2) are connected as one through a mortise and tenon structure (1-4).

3. The fully automated DNA purification and amplification device for solid samples according to claim 1, characterized in that: The centrifugal module (3-2) includes: A cabin (5-3), the cabin (5-3) is a cylindrical structure, and a movable cabin door is provided on the top cover of the cabin (5-3); A hanging basket (5-1), the hanging basket (5-1) comprising a first hanging basket body and a second hanging basket body, the first hanging basket body and the second hanging basket body being arranged on a horizontal rotor (5-2), the first hanging basket body and the second hanging basket body being provided with a sample plate positioner and a matching plate positioner, respectively used for placing the sample plate and the matching plate; a horizontal rotor (5-2), the horizontal rotor (5-2) being rotatably arranged on the bottom plate of the cabin body and being used to drive the hanging basket (5-1) to rotate around its center; a rotary drive motor, wherein the output shaft of the rotary drive motor is in transmission connection with the horizontal rotor (5-2) and is used to drive the horizontal rotor to rotate around its center; A door drive motor, the output shaft of which is in transmission connection with the door for opening or closing the door; The photoelectric sensor is arranged on the inner wall of the cabin and is used to sense that the first hanging basket body or the second hanging basket body has reached the inlet or outlet position of the cabin.

4. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: It also includes a liquid processing module (3-6) and a balancing plate (3-14), wherein the liquid processing module (3-6) is used to inject liquid into the balancing plate (3-14), and the balancing plate (3-14) is used to balance the centrifugal module (3-2).

5. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: It also includes a sample adding tip (3-13) and a multi-channel pipetting module (3-9), wherein the sample adding tip (3-13) is loaded by the multi-channel pipetting module (3-9) and is used to transfer the lysate; the multi-channel pipetting module (3-9) is driven by a robotic arm (3-18).

6. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: It also includes a DNA preservation plate (3-12) and a PCR plate (3-11), and the plate moving module (3-7) moves the DNA preservation plate (3-12) and the PCR plate (3-11) to the sealing module (3-1) according to settings.

7. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: Also included is a waste channel (3-17), wherein the waste channel (3-17) is used for loading waste.

8. The fully automated DNA purification and amplification device for solid samples according to claim 1, characterized in that: It also includes a cover opening module (3-16), which is used to open and close the test tube cover.

9. The fully automated solid sample DNA purification and amplification device according to claim 1, characterized in that: It also includes a barcode reader (3-15), which is used to scan the barcodes on the sample plate and the sample tip (3-13) to record the consumables information.

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