Protein peptide and polysaccharide efficient separation equipment for sea cucumber peptide processing and use method of protein peptide and polysaccharide efficient separation equipment

Abstract

The invention relates to protein peptide and polysaccharide efficient separation equipment for sea cucumber peptide processing and a use method of the protein peptide and polysaccharide efficient separation equipment, and particularly relates to the technical field of bioengineering. The sea cucumbers are subjected to two-time enzymolysis by adopting two-time enzyme adding and two-time different hydrolase adding in cooperation with different enzymolysis conditions, a first ultrafiltration membrane with the molecular weight cut-off of 10 KDa is selected for first-time ultrafiltration separation, permeate liquid is protein peptide mixed liquid, cut-off liquid is polysaccharide mixed liquid, and the protein peptide mixed liquid and the polysaccharide mixed liquid are mixed. The protein peptide mixed solution and the polysaccharide mixed solution correspondingly enter corresponding ultrafiltration assemblies to be subjected to multi-stage ultrafiltration, protein peptide and polysaccharide are further separated and purified respectively, and finally the protein peptide mixed solution and the polysaccharide mixed solution enter corresponding spray drying towers to be subjected to spray drying to obtain corresponding protein peptide powder and polysaccharide powder; separation, purification and drying of protein peptide and polysaccharide can be achieved at the same time, and waste of resources is reduced.

Description

Technical Field

[0001] The present invention relates to the field of bioengineering technology, and in particular to a protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing and a use method thereof. Background Art

[0002] Sea cucumber peptides are a type of bioactive substance extracted from sea cucumbers, which have a variety of physiological functions that are beneficial to the human body. They are usually composed of 2 to 20 amino acids, with a molecular weight below 2000Da, and are rich in glutamic acid, glycine and proline. Sea cucumber peptides are widely used in functional foods, medicines and skin care products due to their good solubility, stability and easy absorption by the human body. The biological activities of sea cucumber peptides include antioxidant, anti-inflammatory, anti-cancer, anti-fatigue, immunomodulatory, anti-bacterial, anti-diabetic, anti-obesity, blood pressure reduction and anti-aging. They can exert their effects through a variety of mechanisms, such as by increasing the activity of antioxidant enzymes, regulating cell signaling pathways, and promoting cell proliferation and differentiation. In the food industry, sea cucumber peptides are valued for their high absorption rate and significant biological functions. They can be used as nutritional supplements to help postoperative patients recover, enhance human immunity, inhibit tumor cell growth and metastasis, and can also be used as anti-fatigue food additives in food manufacturing for athletes and people prone to fatigue.

[0003] At present, most of the extraction of sea cucumber peptides still adopts a one-time enzymatic hydrolysis method, which can only achieve the extraction of protein peptides, but cannot achieve the extraction of sea cucumber polysaccharides at the same time as the extraction of protein peptides, resulting in a waste of resources. Summary of the invention

[0004] (1) Technical solution

[0005] In order to solve the above technical problems, the present invention provides a protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing, comprising a quick freezer, a slicing and grinding mechanism arranged at the discharge end of the quick freezer, an enzymatic hydrolysis mechanism, a separation mechanism, two ultrafiltration components, and two spray drying mechanisms, the discharge end of the slicing and grinding mechanism is connected to the feed end of the enzymatic hydrolysis mechanism through a pipeline, the discharge end of the enzymatic hydrolysis mechanism is connected to the feed end of the separation mechanism through a pipeline, the separation mechanism comprises a separation tank, a first ultrafiltration membrane capable of separating protein peptides from polysaccharides is provided in the separation tank, one side above the first ultrafiltration membrane is connected to the feed end of one of the ultrafiltration components through a retentate discharge pipe, a liquid outlet is provided at the bottom of the separation tank, the liquid outlet is connected to the feed end of another ultrafiltration component through a pipeline, and the discharge ends of the two ultrafiltration components are respectively connected to the feed ends of the two spray drying mechanisms.

[0006] Preferably, the slicing and grinding mechanism includes a machine table, a cutting bracket, a sleeve, a cylinder, a piston rod, a cutting blade, a grinding roller, and a discharge pipe. The cutting bracket in an inverted L shape is fixed on the machine table, the sleeve is provided on the bottom surface of the cross arm of the cutting bracket, the cylinder is fixed on the inner wall of the upper end of the sleeve, the piston rod of the cylinder is arranged downward and the lower end passes through the lower end of the sleeve and is connected to the top of the blade mounting frame, the cutting blade is installed in the blade mounting frame, a pair of grinding rollers are installed in the machine table, a slideway inclined toward the grinding roller direction is opened on the machine table, and a discharge port is provided below the grinding roller.

[0007] Preferably, sliding blocks are provided on both sides of the piston rod, and a sliding groove slidably connected to the sliding blocks is provided on the inner wall of the sleeve.

[0008] Preferably, the enzymolysis mechanism includes an enzymolysis tank, a stirring shaft, a stirring motor, an enzyme addition port, and a discharge port. The discharge port is connected to the feed port of the enzymolysis tank through a discharge pipe. The stirring shaft is vertically installed on the inner wall of the top of the enzymolysis tank so as to rotate downward. The stirring shaft is driven by the stirring motor. The discharge port is provided at the bottom of the enzymolysis tank, a first control valve is provided on the discharge port, and the enzyme addition port is provided on the top of the enzymolysis tank.

[0009] Preferably, a liquid inlet is provided on the top of the separation tank, the discharge port is connected to the liquid inlet through a pipeline, and a first delivery pump is provided on the pipeline connecting the discharge port and the liquid inlet.

[0010] Preferably, the ultrafiltration component comprises a plurality of filter cartridges arranged side by side, the plurality of filter cartridges are connected end to end via pipelines, and a second ultrafiltration membrane is arranged in the filter cartridges.

[0011] Preferably, the pore sizes of the plurality of second ultrafiltration membranes in the ultrafiltration component are arranged to decrease in sequence from the feed end to the discharge end of the ultrafiltration component.

[0012] Preferably, the spray drying mechanism includes a spray drying tower, a spray pipe, a cyclone guide plate, and a discharge port. The spray pipe is provided in the spray drying tower, a nozzle is provided on the spray pipe, the discharge end of the ultrafiltration component is connected to the spray pipe through a pipeline, the cyclone guide plates are provided on both sides of the inner wall of the spray drying tower below the spray pipe, the hot air inlet pipe is connected to both sides of the outer wall of the spray drying tower below the cyclone guide plates, a discharge port is provided at the bottom of the spray drying tower, and a third control valve is provided on the discharge port.

[0013] Preferably, a second control valve is provided on the liquid outlet, a second delivery pump is provided on the pipeline connecting the liquid outlet to the feed end of another ultrafiltration component, and a third delivery pump and a fifth control valve are provided on the pipeline connecting the discharge end of the ultrafiltration component to the spray pipe.

[0014] Preferably, the method for using the protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing comprises the following steps:

[0015] (1) Quick freezing: placing fresh sea cucumbers in a quick freezer for quick freezing;

[0016] (2) Slicing: The quick-frozen sea cucumber falls onto the machine, and the cylinder is started to drive the piston rod down and then drive the cutting blade to slice the sea cucumber;

[0017] (3) Grinding: The sliced ​​sea cucumber is pushed into a slide and moves along the slide between a pair of grinding rollers, and a motor driving the grinding rollers is started to drive the grinding rollers to rotate to grind the sliced ​​sea cucumber;

[0018] (4) Enzymolysis: The ground sea cucumber enters the enzymolysis tank through the discharge pipe and 50-70 times of pure water is added, the stirring motor is started to drive the stirring shaft to rotate to achieve full mixing of the sea cucumber powder and water, and enzymes are added twice through the enzyme addition port. The first time, trypsin is added, and the amount of trypsin added is 0.3-5% of the weight of the raw material. The pH is adjusted to 6.5-8.0, the temperature is 45-65°C, and the enzymolysis is carried out for 1-2 hours; the second time, papain is added, and the amount of papain added is 0.4-5% of the weight of the raw material. The pH is adjusted to 4.5-7.0, the temperature is 45-65°C, and the enzymolysis is carried out for 1-2 hours. After the enzymolysis is completed, the enzyme is heated to inactivate the enzyme, and the enzymolysis solution is prepared for stand-alone use;

[0019] (5) Separation for the first time: Open the first control valve, the sea cucumber enzymatic hydrolysate enters the separation tank under the action of the first delivery pump, and under ultrafiltration conditions, a first ultrafiltration membrane with a molecular weight cut-off of 10 KDa is selected to perform ultrafiltration separation, the permeate is a protein peptide mixture, and the intercepted liquid is a polysaccharide mixture;

[0020] (6) Second separation: Open the second control valve, and the protein peptide mixture enters one of the ultrafiltration components under the action of the second delivery pump for multi-stage ultrafiltration to separate the protein peptide and impurities; open the fourth control valve, and the polysaccharide mixture enters another ultrafiltration component for multi-stage ultrafiltration to separate the polysaccharide and impurities;

[0021] (7) Spray drying: Open the fifth control valve, and the separated and purified protein peptides and polysaccharides enter the corresponding spray drying towers under the action of the third delivery pump and are sprayed out from the nozzles. Hot air is delivered to the spray drying towers through the hot air inlet pipe to dry the separated and purified protein peptide and polysaccharide solutions to obtain protein peptide powder and polysaccharide powder respectively.

[0022] (2) Beneficial effects

[0023] The present invention provides a protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing and a method for using the same. Compared with the prior art, the present invention has the following beneficial effects:

[0024] (1) The sea cucumber is hydrolyzed twice by adding different hydrolases twice in combination with different hydrolysis conditions, and a first ultrafiltration membrane with a molecular weight cutoff of 10 KDa is selected for the first ultrafiltration separation, wherein the permeate is a protein-peptide mixture, and the intercepted liquid is a polysaccharide mixture. The protein-peptide mixture and the polysaccharide mixture enter corresponding ultrafiltration components for multi-stage ultrafiltration, and the protein-peptide and polysaccharide are further separated and purified respectively, and finally enter corresponding spray drying towers for spray drying to obtain corresponding protein-peptide powder and polysaccharide powder, thereby achieving separation, purification and drying of protein-peptide and polysaccharide at the same time, thereby reducing waste of resources.

[0025] (2) The pore sizes of the multiple second ultrafiltration membranes in the ultrafiltration component are arranged to decrease in sequence from the feed end to the discharge end of the ultrafiltration component. Such an arrangement realizes multi-stage hierarchical ultrafiltration, thereby improving the purity and extraction efficiency of sea cucumber protein peptides and sea cucumber polysaccharides.

[0026] (4) The design of the cyclone guide plate enables the hot air to form an upward cyclone, so that the material and liquid are in full contact and the reaction time is long, thus ensuring the quality of the sea cucumber protein peptide and sea cucumber polysaccharide particles. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a structural schematic diagram of the present invention.

[0028] Figure 2 It is a schematic diagram of the cross-sectional structure of the separation tank of the present invention.

[0029] Figure 3 It is a schematic diagram of the cross-sectional structure of the ultrafiltration component of the present invention.

[0030] Figure 4 It is a schematic diagram of the cross-sectional structure of the spray drying tower of the present invention.

[0031] The accompanying drawings are marked as follows: 1-quick freezer, 2-slice grinding mechanism, 21-machine table, 211-slideway, 22-cutting bracket, 23-sleeve, 24-cylinder, 241-piston rod, 25-cutting blade, 251-blade mounting frame, 26-grinding roller, 27-discharging pipe, 3-enzyme hydrolysis mechanism, 31-enzyme hydrolysis tank, 311-feeding port, 32-stirring shaft, 33-stirring motor, 34-enzyme adding port, 35-discharging port, 351-first control valve, 4-separation mechanism, 41-separation tank, 4 2-first ultrafiltration membrane, 43-liquid inlet, 44-liquid outlet, 45-second control valve, 5-ultrafiltration component, 51-filter cartridge, 52-second ultrafiltration membrane, 6-retentate discharge pipe, 7-spray drying mechanism, 71-spray drying tower, 72-spray pipe, 721-nozzle, 73-cyclone guide plate, 74-discharge port, 75-third control valve, 8-hot air inlet pipe, 9-first delivery pump, 10-second delivery pump, 11-third delivery pump, 12-fourth control valve, 13-fifth control valve. DETAILED DESCRIPTION

[0032] The present invention is further described in conjunction with the accompanying drawings and embodiments.

[0033] like Figures 1 to 4 As shown, a protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing described in the present invention comprises a quick freezer 1, a slicing and grinding mechanism 2 arranged at the discharge end of the quick freezer 1, an enzymatic hydrolysis mechanism 3, a separation mechanism 4, two ultrafiltration components 5, and two spray drying mechanisms 7, wherein the discharge end of the slicing and grinding mechanism 2 is connected to the feed end of the enzymatic hydrolysis mechanism 3 through a pipeline, and the discharge end of the enzymatic hydrolysis mechanism 3 is connected to the feed end of the separation mechanism 4 through a pipeline, and the separation mechanism 4 comprises a separation tank 41, in which a first ultrafiltration membrane 42 capable of separating protein peptides from polysaccharides is provided, and one side above the first ultrafiltration membrane 42 is connected to the feed end of one of the ultrafiltration components 5 through a retentate discharge pipe 6, and a liquid outlet 44 is provided at the bottom of the separation tank 41, and the liquid outlet 44 is connected to the feed end of another ultrafiltration component 5 through a pipeline, and the discharge ends of the two ultrafiltration components 5 are respectively connected to the feed ends of the two spray drying mechanisms 7.

[0034] The slicing and grinding mechanism 2 includes a machine table 21, a cutting bracket 22, a sleeve 23, a cylinder 24, a piston rod 241, a cutting blade 25, a grinding roller 26, and a discharge pipe 27. The cutting bracket 22 in an inverted L shape is fixed on the machine table 21, the sleeve 23 is provided on the bottom surface of the cross arm of the cutting bracket 22, the cylinder 24 is fixed on the inner wall of the upper end of the sleeve 23, the piston rod 241 of the cylinder 24 is arranged downward and the lower end passes through the lower end of the sleeve 23 and is connected to the top of the blade mounting frame 251, the cutting blade 25 is installed in the blade mounting frame 251, a pair of the grinding rollers 26 are installed in the machine table 21, a slideway 211 inclined toward the grinding roller 26 is opened on the machine table 21, and a discharge port is provided below the grinding roller 26.

[0035] Slide blocks are provided on both sides of the piston rod 241 , and a sliding groove slidably connected to the slide blocks is provided on the inner wall of the sleeve 23 .

[0036] The enzymolysis mechanism 3 includes an enzymolysis tank 31, a stirring shaft 32, a stirring motor 33, an enzyme addition port 34, and a discharge port 35. The discharge port is connected to the feed port 311 of the enzymolysis tank 31 through a discharge pipe 27. The stirring shaft 32 is vertically and downwardly mounted on the inner wall of the top of the enzymolysis tank 31. The stirring shaft 32 is driven by the stirring motor 33. The discharge port 35 is provided at the bottom of the enzymolysis tank 31, and a first control valve 351 is provided on the discharge port 35. The enzyme addition port 34 is provided on the top of the enzymolysis tank 31.

[0037] A liquid inlet 43 is disposed on the top of the separation tank 41 , and the discharge port 35 is connected to the liquid inlet 43 via a pipeline. A first delivery pump 9 is disposed on the pipeline connecting the discharge port 35 and the liquid inlet 43 .

[0038] The ultrafiltration assembly 5 includes a plurality of filter cartridges 51 arranged side by side. The plurality of filter cartridges 51 are connected end to end via pipelines. A second ultrafiltration membrane 52 is disposed in the filter cartridges 51 .

[0039] The pore sizes of the plurality of second ultrafiltration membranes 52 in the ultrafiltration component 5 are arranged to decrease in sequence from the feed end to the discharge end of the ultrafiltration component 5. Such an arrangement realizes multi-stage ultrafiltration to improve the purity of sea cucumber protein peptides and sea cucumber polysaccharides.

[0040] The spray drying mechanism 7 includes a spray drying tower 71, a spray pipe 72, a cyclone guide plate 73, and a discharge port 74. The spray drying tower 71 is provided with the spray pipe 72, and the spray pipe 72 is provided with a nozzle 721. The discharge end of the ultrafiltration component 5 is connected to the spray pipe 72 through a pipeline. The cyclone guide plates 73 are provided on both sides of the inner wall of the spray drying tower 71 below the spray pipe 72. The outer wall of the spray drying tower 71 below the cyclone guide plates 73 is connected with a hot air inlet pipe 8. The design of the cyclone guide plate 73 enables the hot air to form an ascending vortex, so that the feed liquid is fully in contact and the reaction time is long, thereby ensuring the quality of the sea cucumber protein peptide and sea cucumber polysaccharide particles. The bottom of the spray drying tower 71 is provided with a discharge port 74, and the discharge port 74 is provided with a third control valve 75.

[0041] A second control valve 45 is provided on the liquid outlet 44, a second delivery pump 10 is provided on the pipeline connecting the liquid outlet 44 and the feed end of another ultrafiltration component 5, and a third delivery pump 11 and a fifth control valve 13 are provided on the pipeline connecting the discharge end of the ultrafiltration component 5 and the spray pipe 72.

[0042] A method for using a protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing, comprising the following steps:

[0043] (1) Quick freezing: placing fresh sea cucumbers in a quick freezer 1 for quick freezing;

[0044] (2) Slicing: The quick-frozen sea cucumber falls onto the machine table 21, and the cylinder 24 is started to drive the piston rod 241 downward, thereby driving the cutting blade 25 to slice the sea cucumber;

[0045] (3) Grinding: The sliced ​​sea cucumber is pushed into the slideway 211 and enters between a pair of grinding rollers 26 along the slideway 211, and a motor driving the grinding rollers 26 is started to drive the grinding rollers 26 to rotate to grind the sliced ​​sea cucumber;

[0046] (4) Enzymolysis: The ground sea cucumber enters the enzymolysis tank 31 through the discharge pipe 27 and 50-70 times of pure water is added, the stirring motor 33 is started to drive the stirring shaft 32 to rotate to achieve full mixing of the sea cucumber powder and the water, and enzymes are added twice through the enzyme addition port 34. The first time, trypsin is added, and the amount of trypsin added is 0.3-5% of the weight of the raw material. The pH is adjusted to 6.5-8.0, the temperature is 45-65°C, and the enzymolysis is carried out for 1-2 hours; the second time, papain is added, and the amount of papain added is 0.4-5% of the weight of the raw material. The pH is adjusted to 4.5-7.0, the temperature is 45-65°C, and the enzymolysis is carried out for 1-2 hours. After the enzymolysis is completed, the enzyme is heated to inactivate the enzyme, and the enzymolysis solution is prepared for standby use;

[0047] (5) First separation: Open the first control valve 351, and the sea cucumber enzymatic hydrolyzate enters the separation tank 41 under the action of the first delivery pump 9. Under ultrafiltration conditions, the first ultrafiltration membrane 42 with a molecular weight cutoff of 10 KDa is selected to perform ultrafiltration separation, and the permeate is a protein peptide mixture, and the intercepted liquid is a polysaccharide mixture;

[0048] (6) Second separation: Open the second control valve 45, and the protein peptide mixture enters one of the ultrafiltration components 5 under the action of the second delivery pump 10 to perform multi-stage ultrafiltration to separate the protein peptide and impurities; open the fourth control valve 12, and the polysaccharide mixture enters another ultrafiltration component 5 to perform multi-stage ultrafiltration to separate the polysaccharide and impurities;

[0049] (7) Spray drying: Open the fifth control valve 13, and the separated and purified protein peptides and polysaccharides enter the corresponding spray drying tower 71 under the action of the third delivery pump 11 and are sprayed out from the nozzle 721. Hot air is delivered to the spray drying tower 71 through the hot air inlet pipe 8 to dry the separated and purified protein peptide and polysaccharide solutions to obtain protein peptide powder and polysaccharide powder respectively.

[0050] The above-described embodiments only express the preferred embodiments of the present invention, and the description thereof is relatively specific and detailed, but the present invention is not limited to these embodiments. It should be noted that it is obvious to those skilled in the art. Without departing from the purpose of the present invention, any improvement made shall fall within the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A highly efficient separation device for protein peptides and polysaccharides used in sea cucumber peptide processing, characterized in that: The invention comprises a quick freezer (1), a slicing and grinding mechanism (2) arranged at the discharge end of the quick freezer (1), an enzymatic hydrolysis mechanism (3), a separation mechanism (4), two ultrafiltration components (5), and two spray drying mechanisms (7), wherein the discharge end of the slicing and grinding mechanism (2) is connected to the feed end of the enzymatic hydrolysis mechanism (3) via a pipeline, the discharge end of the enzymatic hydrolysis mechanism (3) is connected to the feed end of the separation mechanism (4) via a pipeline, and the separation mechanism (4) comprises a separation tank (41), and the separation tank (41) ) is provided with a first ultrafiltration membrane (42) capable of separating protein peptides from polysaccharides, the upper side of the first ultrafiltration membrane (42) is connected to the feed end of one of the ultrafiltration components (5) through a retentate discharge pipe (6), the bottom of the separation tank (41) is provided with a liquid outlet (44), the liquid outlet (44) is connected to the feed end of another ultrafiltration component (5) through a pipeline, and the discharge ends of the two ultrafiltration components (5) are respectively connected to the feed ends of the two spray drying mechanisms (7).

2. The protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing according to claim 1, characterized in that: The slicing and grinding mechanism (2) comprises a machine table (21), a cutting support (22), a sleeve (23), a cylinder (24), a piston rod (241), a cutting blade (25), a grinding roller (26), and a discharge pipe (27); the machine table (21) is fixed with the cutting support (22) in an inverted L shape; the sleeve (23) is provided on the bottom surface of the cross arm of the cutting support (22); the cylinder (24) is fixed on the inner wall of the upper end of the sleeve (23) The piston rod (241) of the cylinder (24) is arranged downward and the lower end thereof passes through the lower end of the sleeve (23) and is connected to the top of the blade mounting frame (251); the cutting blade (25) is mounted in the blade mounting frame (251); a pair of grinding rollers (26) are mounted in the machine platform (21); a slideway (211) inclined toward the grinding roller (26) is provided on the machine platform (21); and a material discharge port is provided below the grinding roller (26).

3. The protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing according to claim 2, characterized in that: Slide blocks are provided on both sides of the piston rod (241), and a sliding groove slidably connected to the slide blocks is provided on the inner wall of the sleeve (23).

4. The protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing according to claim 2, characterized in that: The enzymolysis mechanism (3) comprises an enzymolysis tank (31), a stirring shaft (32), a stirring motor (33), an enzyme adding port (34), and a discharge port (35); the discharge port is connected to a feed port (311) of the enzymolysis tank (31) via a discharge pipe (27); the stirring shaft (32) is vertically and downwardly mounted on the inner wall of the top of the enzymolysis tank (31); the stirring shaft (32) is driven by the stirring motor (33); the discharge port (35) is provided at the bottom of the enzymolysis tank (31); a first control valve (351) is provided on the discharge port (35); and the enzyme adding port (34) is provided on the top of the enzymolysis tank (31).

5. The protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing according to claim 4, characterized in that: A liquid inlet (43) is provided on the top of the separation tank (41), and the discharge port (35) is connected to the liquid inlet (43) via a pipeline. A first delivery pump (9) is provided on the pipeline connecting the discharge port (35) and the liquid inlet (43).

6. The protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing according to claim 1, characterized in that: The ultrafiltration component (5) comprises a plurality of filter cartridges (51) arranged side by side, the plurality of filter cartridges (51) being connected end to end in an interlaced manner via pipelines, and a second ultrafiltration membrane (52) being arranged in the filter cartridges (51).

7. The protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing according to claim 6, characterized in that: The pore sizes of the plurality of second ultrafiltration membranes (52) in the ultrafiltration component (5) are arranged to decrease in sequence from the feed end to the discharge end of the ultrafiltration component (5).

8. The protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing according to claim 1, characterized in that: The spray drying mechanism (7) comprises a spray drying tower (71), a spray pipe (72), a cyclone guide plate (73), and a discharge port (74). The spray drying tower (71) is provided with the spray pipe (72), the spray pipe (72) is provided with a nozzle (721), the discharge end of the ultrafiltration component (5) is connected to the spray pipe (72) through a pipeline, the cyclone guide plates (73) are provided on both sides of the inner wall of the spray drying tower (71) below the spray pipe (72), the hot air inlet pipe (8) is connected to both sides of the outer wall of the spray drying tower (71) below the cyclone guide plate (73), the discharge port (74) is provided at the bottom of the spray drying tower (71), and the discharge port (74) is provided with a third control valve (75).

9. The protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing according to claim 8, characterized in that: A second control valve (45) is provided on the liquid outlet (44), a second delivery pump (10) is provided on a pipeline connecting the liquid outlet (44) and the feed end of another ultrafiltration component (5), and a third delivery pump (11) and a fifth control valve (13) are provided on a pipeline connecting the discharge end of the ultrafiltration component (5) and the spray pipe (72).

10. A method for using a protein peptide and polysaccharide efficient separation device for sea cucumber peptide processing according to any one of claims 1 to 9, characterized in that: The following steps are involved: (1) Quick freezing: placing fresh sea cucumbers in a quick freezer (1) for quick freezing; (2) Slicing: The quick-frozen sea cucumber falls onto the machine table (21), and the cylinder (24) is started to drive the piston rod (241) to descend, thereby driving the cutting blade (25) to slice the sea cucumber; (3) Grinding: The sliced ​​sea cucumber is pushed into the slideway (211) and enters between a pair of grinding rollers (26) along the slideway (211), and a motor driving the grinding rollers (26) is started to drive the grinding rollers (26) to rotate to grind the sliced ​​sea cucumber; (4) Enzymolysis: The ground sea cucumber enters the enzymolysis tank (31) through the discharge pipe (27) and 50-70 times of pure water is added, the stirring motor (33) is started to drive the stirring shaft (32) to rotate to achieve full mixing of the sea cucumber powder and water, and enzymes are added twice through the enzyme addition port (34). The first time, trypsin is added, and the amount of trypsin added is 0.3-5% of the weight of the raw material. The pH is adjusted to 6.5-8.0, the temperature is 45-65°C, and the enzymolysis is carried out for 1-2 hours; the second time, papain is added, and the amount of papain added is 0.4-5% of the weight of the raw material. The pH is adjusted to 4.5-7.0, the temperature is 45-65°C, and the enzymolysis is carried out for 1-2 hours. After the enzymolysis is completed, the enzyme is heated to inactivate the enzyme, and the enzymolysis solution is prepared for standby use; (5) First separation: Open the first control valve (351), and the sea cucumber hydrolyzate enters the separation tank (41) under the action of the first delivery pump (9). Under ultrafiltration conditions, a first ultrafiltration membrane (42) with a molecular weight cutoff of 10 KDa is selected for ultrafiltration separation, and the permeate is a protein-peptide mixture, and the intercepted liquid is a polysaccharide mixture; (6) Second separation: Open the second control valve (45), and the protein peptide mixture enters one of the ultrafiltration components (5) under the action of the second delivery pump (10) for multi-stage ultrafiltration to separate the protein peptide and impurities; The fourth control valve (12) is opened, and the polysaccharide mixed solution enters another ultrafiltration component (5) for multi-stage ultrafiltration to separate the polysaccharide and impurities; (7) Spray drying: The fifth control valve (13) is opened, and the separated and purified protein peptides and polysaccharides enter the corresponding spray drying tower (71) under the action of the third delivery pump (11) and are sprayed out from the nozzle (721). Hot air is delivered to the spray drying tower (71) through the hot air inlet pipe (8) to dry the separated and purified protein peptide and polysaccharide solutions to obtain protein peptide powder and polysaccharide powder respectively.

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