Leakage-proof efficient capillary centrifugal blood preparation method

Abstract

The invention discloses a leakproof efficient capillary centrifugation blood preparation method, and relates to the technical field of capillary centrifugation, the method comprises the following steps: preparing packed red blood cells to be separated and a capillary; accurately sucking the packed red blood cells in the test tube by using a fixed microsyringe; the two ends of the capillary tube are hot-pressed and plugged through a heat sealing machine and then placed into the sleeve; putting the capillary tube into a high-speed centrifugal machine for centrifugal treatment; according to the method, sample sections with the length of 5 mm at the telecentric end and the proximal end of the capillary tube are cut off through scissors, the capillary tube is put into a pre-marked test tube, red blood cells are washed three times, and follow-up experiments are carried out, the capillary tube is not prone to breakage, the capillary tube is easily and arbitrarily cut off, then the two ends of the capillary tube are blocked through a heat sealing machine, liquid leakage is effectively prevented, and the capillary tube is convenient to use. The risk of cross contamination is reduced, and the preparation method ensures the consistency of experimental conditions each time through a standardized operation process, and improves the repeatability of experimental results.

Description

Technical Field

[0001] The invention relates to the technical field of capillary centrifugation, in particular to a leak-proof and efficient capillary centrifugation blood preparation method. Background Art

[0002] Capillary centrifugation technology is widely used in the biomedical field, especially in blood processing; this technology is mainly used to separate blood components, such as red blood cells, white blood cells and plasma; with the continuous development of medical technology, higher requirements are placed on the safety, reliability and ease of operation of centrifugal equipment.

[0003] However, existing capillary centrifugation devices are still insufficient in some aspects, especially when processing sensitive samples such as blood, where preventing sample contamination and loss becomes a key issue.

[0004] At present, the commonly used capillary centrifugation method includes the following steps: first, the experimental sample is prepared, then a dedicated capillary centrifuge is used to centrifuge and separate the sample components for subsequent testing and research. Although this traditional method can achieve blood separation to a certain extent, there are many problems in actual operation:

[0005] (1) Problems with capillary plugging materials:

[0006] Prior art: Currently, the commonly used plugging material is plasticine.

[0007] Disadvantages: If the plasticine is not sealed tightly, it is easy to cause liquid leakage, which will not only cause sample loss, but also may contaminate equipment or the working environment, increasing the risk of cross-contamination. In addition, the use of plasticine is cumbersome and it is difficult to ensure the consistency of each seal.

[0008] (2) Capillary material problem:

[0009] Prior art: Traditional capillaries are mostly made of glass.

[0010] Disadvantages: Although glass capillaries are highly transparent, they are brittle and easy to break. They are also difficult to process into specific shapes to facilitate sample collection. Especially when it is necessary to obtain 5 mm samples from both ends of the capillary, the glass material is difficult to operate, increasing the possibility of operational errors.

[0011] (3) Problems with the liquid suction device:

[0012] Prior art: A rubber-tipped dropper is usually used to absorb liquid.

[0013] Disadvantages: The precision of the rubber-tipped dropper is limited, which is easy to introduce human errors, especially when the amount of suction needs to be precisely controlled. In addition, the efficiency of the rubber-tipped dropper is low and it is not suitable for large-scale sample processing.

[0014] In order to solve the above problems, the present invention provides a leak-proof and efficient capillary centrifugal blood preparation method. Summary of the invention

[0015] In view of the deficiencies of the prior art, the present invention provides a leak-proof and efficient capillary centrifugation blood preparation method, which solves the problems raised by the above-mentioned background technology.

[0016] To achieve the above objectives, the present invention is implemented through the following technical solutions: a leak-proof and efficient capillary centrifugal blood preparation method, the preparation method comprising the following steps:

[0017] Step 1: Sample preparation

[0018] ①Prepare the packed red blood cells to be separated and place them in a test tube for later use;

[0019] ②Prepare capillaries for aspirating packed red blood cells and set aside;

[0020] Step 2: Sample preparation

[0021] ①Insert the prepared capillary into the micro-injector and fix it;

[0022] ②Insert one end of the capillary into the test tube, and use a fixed micro-syringe to accurately absorb the packed red blood cells in the test tube, and absorb a certain amount of packed red blood cells into the capillary;

[0023] Step 3: Blocking treatment

[0024] ① After the packed red blood cells are absorbed into the capillary, separate the capillary from the micro-syringe, and then use a heat sealing machine to heat-press and seal both ends of the capillary to ensure complete sealing;

[0025] ②Then put the hot-pressed capillary into the casing to protect it;

[0026] Step 4: Centrifugation

[0027] Place the cannula with built-in capillary into a dedicated capillary high-speed centrifuge for centrifugal treatment;

[0028] Step 5: Sample post-processing

[0029] After centrifugation is completed, remove the capillary from the sleeve of the centrifuge, use scissors to cut off 5 mm sample segments at both ends of the distal and proximal ends of the capillary, put them into pre-labeled test tubes, wash the red blood cells 3 times, and then proceed with subsequent experiments.

[0030] As a further technical solution of the present invention, the capillary preparation conditions in step 1 are:

[0031] ①Use medical grade plastic materials to prepare capillaries through one-piece injection molding through the cooperation of injection molding machine and mold;

[0032] ② One end of the capillary is in a conical trumpet-shaped state, and the whole is an integrated transparent structure, and its size matches and is compatible with the micro-syringe, while the other end of the capillary is open.

[0033] As a further technical solution of the present invention, the medical grade plastic material used in the capillary is polypropylene or polyethylene.

[0034] As a further technical solution of the present invention, the sleeve is a hard plastic tube made of a relatively hard material.

[0035] As a further technical solution of the present invention, the capillary centrifugation conditions in step 4 are: centrifugation at 10000 rpm / 10 min for 3-10 min.

[0036] As a further technical solution of the present invention, the heat sealing machine adopts the heat sealing machine equipment commonly used in blood stations, which can quickly heat-seal and block the two ends of the capillary to prevent any liquid leakage.

[0037] The present invention provides a leak-proof and efficient capillary centrifugal blood preparation method. Compared with the prior art, it has the following beneficial effects:

[0038] 1. A leak-proof and efficient capillary centrifugal blood preparation method, which uses medical-grade plastic capillaries, which are not only highly transparent, but also resistant to chemical corrosion, have good mechanical strength, are not easy to break, and are easy to cut the capillaries arbitrarily, thereby improving the safety and reliability of sample processing. Secondly, one end of the capillary is designed to be trumpet-shaped and the other end is open, which not only ensures matching and compatibility with micro-syringes, facilitates the transfer of samples into the capillary, but also improves the accuracy of sampling.

[0039] 2. A leak-proof and efficient capillary centrifugal blood preparation method. The plastic capillaries are more durable than glass capillaries, which reduces the frequency of replacement due to breakage, thereby reducing the cost of long-term use. Secondly, a heat sealing machine is used to seal both ends of the capillaries, which effectively prevents liquid leakage, improves sealing performance, reduces the risk of cross contamination, and ensures the accuracy of experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 A flow chart of a leak-proof and efficient capillary centrifugal blood preparation method;

[0041] Figure 2 The figure is a schematic diagram of the assembly of a capillary and a micro-syringe in a leak-proof and efficient capillary centrifugal blood preparation method. DETAILED DESCRIPTION

[0042] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0043] See also Figure 1 As shown, the present invention provides a technical solution for a leak-proof and efficient capillary centrifugal blood preparation method: a leak-proof and efficient capillary centrifugal blood preparation method, the preparation method comprising the following steps:

[0044] Step 1: Sample preparation

[0045] ①Prepare the packed red blood cells to be separated and place them in a test tube for later use;

[0046] ②Prepare capillaries for aspirating packed red blood cells and set aside;

[0047] Among them, the capillary preparation conditions are:

[0048] ①Use medical grade plastic materials to prepare capillaries through one-piece injection molding through the cooperation of injection molding machine and mold;

[0049] ② One end of the capillary is in a conical trumpet state, and the whole is an integrated transparent structure. Its size matches and is compatible with the micro-syringe, and the other end of the capillary is open. When in use, it is convenient to use the micro-syringe to accurately absorb the packed red blood cells in the test tube into the capillary, which not only facilitates the transfer of the sample to the capillary, but also improves the accuracy of sampling (such as Figure 2 As shown, a in the figure is the capillary).

[0050] Furthermore, the medical-grade plastic material used for the capillary is polypropylene or polyethylene, which not only has good transparency, but also has high chemical resistance and mechanical strength, is not easy to break, and is convenient for subsequent arbitrary cutting of the capillary, thereby improving the safety and reliability of sample processing.

[0051] Step 2: Sample preparation

[0052] ①Insert the prepared capillary into the micro-injector and fix it;

[0053] ②Insert one end of the capillary into the test tube, and use a fixed micro-syringe to accurately absorb the packed red blood cells in the test tube, and absorb a certain amount of packed red blood cells into the capillary; the use of a micro-syringe can accurately control the volume of the absorbed liquid, greatly improving the accuracy of sampling.

[0054] Step 3: Blocking treatment

[0055] ① After the packed red blood cells are absorbed into the capillary, separate the capillary from the micro-syringe, and then use a heat sealer to heat-press and seal both ends of the capillary to ensure complete sealing, effectively prevent liquid leakage, and improve sealing performance. In addition, good sealing performance reduces the possibility of liquid leakage, reduces the risk of cross contamination, and ensures the accuracy of experimental results;

[0056] Among them, the heat sealing machine adopts the heat sealing machine equipment commonly used in blood stations, which can quickly heat-seal the two ends of the capillary tube to prevent any liquid leakage;

[0057] ②Then put the hot-pressed capillary into the casing to protect it;

[0058] Furthermore, the casing is made of a hard plastic tube made of a relatively hard material;

[0059] Step 4: Centrifugation

[0060] Place the cannula with built-in capillary into a dedicated capillary high-speed centrifuge and centrifuge at 10,000 rpm / 10 min for 3-10 min. After centrifugation, the capillary is not damaged or leaking.

[0061] Step 5: Sample post-processing

[0062] After centrifugation is completed, remove the capillary from the sleeve of the centrifuge, use scissors to cut off 5 mm long sample segments at both ends of the distal and proximal ends of the capillary, put them into pre-labeled test tubes, wash the red blood cells three times, and perform Rh (D) antigen testing on the blood samples at the distal and proximal ends, and obtain ideal results.

[0063] The method designed in the present invention ensures the consistency of experimental conditions each time through standardized operating procedures, improves the repeatability of experimental results, and is particularly suitable for large-scale blood sample processing needs of blood stations and blood transfusion departments of clinical hospitals, improves work efficiency, and meets the high-throughput requirements in clinical diagnosis and research.

[0064] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0065] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A leak-proof and efficient capillary centrifugal blood preparation method, characterized in that: The preparation method comprises the following steps: Step 1: Sample preparation ①Prepare the packed red blood cells to be separated and place them in a test tube for later use; ②Prepare capillaries for aspirating packed red blood cells and set aside; Step 2: Sample preparation ①Insert the prepared capillary into the micro-injector and fix it; ②Insert one end of the capillary into the test tube, and use a fixed micro-syringe to accurately absorb the packed red blood cells in the test tube, and absorb a certain amount of packed red blood cells into the capillary; Step 3: Blocking treatment ① After the packed red blood cells are absorbed into the capillary, separate the capillary from the micro-syringe, and then use a heat sealing machine to heat-press and seal both ends of the capillary to ensure complete sealing; ②Then put the hot-pressed capillary into the casing to protect it; Step 4: Centrifugation Place the cannula with built-in capillary into a dedicated capillary high-speed centrifuge for centrifugal treatment; Step 5: Sample post-processing After centrifugation is completed, remove the capillary from the sleeve of the centrifuge, use scissors to cut off 5 mm sample segments at both ends of the distal and proximal ends of the capillary, put them into pre-labeled test tubes, wash the red blood cells 3 times, and then proceed with subsequent experiments.

2. The leak-proof and efficient capillary centrifugation blood preparation method according to claim 1, characterized in that: The capillary preparation conditions in step 1 are: ①Use medical grade plastic materials to prepare capillaries through one-piece injection molding through the cooperation of injection molding machine and mold; ② One end of the capillary is in a conical trumpet-shaped state, and the whole is an integrated transparent structure, and its size matches and is compatible with the micro-syringe, while the other end of the capillary is open.

3. The leak-proof and efficient capillary centrifugation blood preparation method according to claim 2, characterized in that: The medical grade plastic material used for the capillary is polypropylene or polyethylene.

4. The leak-proof and efficient capillary centrifugation blood preparation method according to claim 1, characterized in that: The sleeve is a hard plastic tube made of a relatively hard material.

5. The leak-proof and efficient capillary centrifugation blood preparation method according to claim 1, characterized in that: The capillary centrifugation condition in step 4 is: centrifugation at 10000 rpm / 10 min for 3-10 min.

6. The leak-proof and efficient capillary centrifugation blood preparation method according to claim 1, characterized in that: The heat sealer adopts the heat sealer equipment commonly used in blood stations, which can quickly heat-seal and block the two ends of the capillary tube to prevent any liquid leakage.

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