A method for detecting the astaxanthin content in eggs

Abstract

The present invention relates to the technical field of parameter detection, and specifically to a method for detecting the astaxanthin content in eggs. The method includes obtaining first data; equally dividing and grouping a pre-prepared sample egg liquid to obtain a first measurement egg liquid to the k measurement egg liquid; extracting and smearing to obtain a first comparison sample to the k comparison sample; measuring the astaxanthin content of the first measurement egg liquid to the k measurement egg liquid by liquid chromatography, and fitting to obtain a first measured astaxanthin content curve to the k measured astaxanthin content curve; collecting a first sample image group to the k sample image group; extracting, extracting, smearing, and collecting from the egg liquid to be tested to obtain a first group of images to be tested; according to the first group of images to be tested and the first sample image group to the k sample image group, using a color difference comparison algorithm to calculate the astaxanthin content curve of the egg liquid to be tested. The present invention realizes the rapid detection of the astaxanthin content in eggs by using the idea of astaxanthin color difference comparison.

Description

Technical Field

[0001] The present invention relates to the technical field of parameter detection, and specifically to a method for detecting the astaxanthin content in eggs. Background Art

[0002] The detection of astaxanthin content has always been a technical problem in the field of chemical component detection. The existing relatively mature detection techniques mainly focus on detecting the astaxanthin content in cosmetics, and usually the liquid chromatography method is adopted. However, if the liquid chromatography method is applied to the detection of egg astaxanthin, there are still the following problems: First, since the detection process of the liquid chromatography method is relatively cumbersome and the detection environment is also relatively harsh, it is only applicable to the detection of a small number of samples. Since the components of cosmetics are relatively stable, it is a feasible detection method to use the liquid chromatography method to detect a small number of cosmetic samples and then estimate the astaxanthin content in cosmetics. However, for eggs, since eggs are naturally formed and the astaxanthin content in each egg may vary greatly, in order to estimate the astaxanthin content of a batch of eggs, it is necessary to quickly detect the astaxanthin in a large number of samples. This leads to the traditional liquid chromatography detection method being unable to meet the requirements for detecting the astaxanthin content in eggs. Second, since astaxanthin will undergo redox reactions when exposed to air, resulting in a gradual decrease in the astaxanthin content. Therefore, in order to comprehensively reflect the astaxanthin content in eggs, it is necessary to measure the change curve of the astaxanthin content with the exposure time. It is very difficult for the existing liquid chromatography detection method to achieve this requirement.

[0003] Therefore, it is necessary to adopt a new measurement method and use big data algorithms to achieve the rapid detection of astaxanthin in eggs. Summary of the Invention

[0004] (1) Technical Problems to be Solved

[0005] The purpose of the present invention is to provide a method for detecting the astaxanthin content in eggs to achieve the rapid detection of the change curve of the egg astaxanthin content with the exposure time.

[0006] (2) Technical Solutions

[0007] To achieve the above purpose, the present invention provides a method for detecting the astaxanthin content in eggs, and the method includes the following steps:

[0008] S1, obtaining first data; the first data includes a preset measurement period, a preset first interval time, a preset first weight, a preset second weight, and a preset third weight.

[0009] S2, according to the preset number of sample groups M , equally dividing the pre-prepared sample egg liquid into the first group of egg liquid to the M group of egg liquid; according to the number of sample groupsM The calculated mixing quantity k ; The first grouped egg liquid to the M grouped egg liquids are grouped and mixed to obtain the first measured egg liquid to the k measured egg liquids; Egg liquids with a weight of the first weight are respectively extracted from the first measured egg liquid to the k measured egg liquids to obtain the first comparison egg liquid to the k comparison egg liquids; The extraction method is used to extract astaxanthin from the first comparison egg liquid to the k comparison egg liquids to obtain the first comparison solution to the k comparison solutions; The weights of the first comparison solution to the k comparison solutions are all the third weight; The first comparison solution to the k comparison solutions are evenly smeared on the pre-set first white test paper to the k surface of the white test paper to obtain the first comparison sample to the k comparison samples.

[0010] S3. Taking the first interval time as the time interval, within the measurement period, the liquid chromatography method is used to measure the astaxanthin content of the first measured egg liquid to the k measured egg liquids, and the first measured astaxanthin content curve to the k measured astaxanthin content curve is fitted; Taking the first interval time as the time interval, within the measurement period, the images of the first comparison sample to the k comparison samples are collected to obtain the first sample image group to the k sample image group.

[0011] S4. Egg liquids with a weight of the first weight are extracted from the egg liquid to be tested to obtain the first test egg liquid; The extraction method is used to extract astaxanthin from the first test egg liquid to obtain the first test solution; The weight of the first test solution is the third weight; The first test solution is evenly smeared on the surface of the pre-set test white test paper to obtain the first test sample; The images of the first test sample are collected at the first interval time to obtain the first test image group; According to the first test image group and the first sample image group to the k sample image group, the astaxanthin content curve of the test egg liquid is calculated by using the color difference comparison algorithm.

[0012] Further, the method for M calculating the mixing quantity according to the sample grouping quantity k includes:

[0013] According to the sample grouping quantity M , the mixing quantity is calculated by using the mixing calculation formula to obtain the mixing quantity k; The mixing calculation formula is as follows:

[0014] ;

[0015] Among them, represents the floor function symbol.

[0016] Furthermore, the method of grouping and mixing the first group of egg liquid to the M group of egg liquid to obtain the first measured egg liquid to the k measured egg liquid includes:

[0017] Extract astaxanthin from the first group of egg liquid to the group of egg liquid to obtain the first extraction solution to the extraction solution; The weights of the first extraction solution to the extraction solution are all the second weight; The extraction solution to the extraction solution is mixed with the group of egg liquid, Traverse the integers between 2 and to obtain the second measured egg liquid to the k measured egg liquid; Among them and The calculation formula of is:

[0018] ;

[0019] Record the group of egg liquid as the first measured egg liquid.

[0020] Furthermore, with the first time interval as the time interval, the astaxanthin content of the first measured egg liquid to the k measured egg liquid is measured by liquid chromatography within the measurement period, and the first measured astaxanthin content curve to the k measured astaxanthin content curve is fitted. The method includes:

[0021] With the first time interval as the time interval, the astaxanthin content of the first measured egg liquid to the k measured egg liquid is measured by liquid chromatography within the measurement period to obtain the first measured astaxanthin content time series to the k measured astaxanthin content time series; The astaxanthin content of the first measured egg liquid to the k measured egg liquid is in milligrams per kilogram.

[0022] Use the least squares fitting algorithm to respectively fit the first measured astaxanthin content time series to the kFitting the time series of astaxanthin content measurements to obtain the first astaxanthin content measurement curve to the k astaxanthin content measurement curve; defining the independent variable of the first astaxanthin content measurement curve to the k astaxanthin content measurement curve as the astaxanthin exposure time .

[0023] Furthermore, the method for calculating the astaxanthin content curve of the egg liquid to be measured by using the color difference comparison algorithm according to the first group of images to be measured and the first group of sample images to the k group of sample images includes:

[0024] Evenly dividing each frame of the images in the first group of images to be measured to obtain image segments to be measured; where is the preset number of horizontal divisions, is the preset number of vertical divisions, is the sequence number; where The calculation formula of is:

[0025] ;

[0026] Among them, represents the measurement period; represents the first interval time.

[0027] Label image segments to be measured in sequence as ; where is an integer variable with values from 1 to , is an integer variable with values from 1 to , is an integer variable with values from 1 to .

[0028] Evenly divide each frame of the images in the first group of sample images to the k group of sample images to obtain image segments of the sample; label image segments of the sample in sequence as ; where is an integer variable with values from 1 to .

[0029] Use a color difference meter to measure the tristimulus values of image segments to be measured respectively to obtain , , ; Use a color difference meter to measure the tristimulus values of image segments of the sample respectively to obtain , , .

[0030] Match , , with , , to obtain the first similarity , the second similarity , the first matching serial number and the second matching serial number .

[0031] Calculate the astaxanthin content curve of the egg liquid to be measured based on the first similarity, the second similarity, the first matching serial number, the second matching serial number, the first measured astaxanthin content curve to the k measured astaxanthin content curve.

[0032] Furthermore, the method of matching , , with , , to obtain the first similarity , the second similarity , the first matching serial number and the second matching serial number includes:

[0033] Calculate the tristimulus distance sequence according to , , and , , ; the calculation formula of the tristimulus distance sequence is:

[0034] ;

[0035] where represents the th element in the tristimulus distance sequence.

[0036] Search for the two smallest values in the tristimulus distance sequence, denoted as , ; Denote the element numbers corresponding to , in the tristimulus distance sequence as the first matching serial number and the second matching serial number respectively.

[0037] According to , ​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​, the first similarity and the second similarity are calculated using a similarity algorithm.

[0038] Further, the method of calculating the first similarity and the second similarity according to , includes:

[0039] According to , calculate the first similarity and the second similarity; the calculation formulas for the first similarity and the second similarity are:

[0040] .

[0041] Further, the method of calculating the astaxanthin content curve of the egg liquid to be measured according to the first similarity, the second similarity, the first matching serial number, the second matching serial number, the first measured astaxanthin content curve to the k measured astaxanthin content curve includes:

[0042] According to the first similarity, the second similarity, the first matching serial number, the second matching serial number, the first measured astaxanthin content curve to the k measured astaxanthin content curve, use the curve weighting formula to calculate the astaxanthin content curve of the egg liquid to be measured; the curve weighting formula is:

[0043] ;

[0044] Among them, represents the astaxanthin content curve of the egg liquid to be measured, represents the measured astaxanthin content curve, represents the measured astaxanthin content curve.

[0045] (3) Beneficial effects

[0046] Compared with the prior art, the beneficial effects of the present invention are:

[0047] Based on the color characteristics of astaxanthin oxidation-reduction, by collecting the tristimulus value data of the first group of images to be measured and the first group of sample images to the k group of sample images, use the color difference comparison algorithm to calculate the astaxanthin content curve of the egg liquid to be measured. Description of the drawings

[0048] Figure 1 is a flowchart of a method for detecting the astaxanthin content in eggs according to Embodiment 1 of the present invention. Specific implementation manners

[0049] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0050] Before giving examples, it is necessary to elaborate on the application scenario of the concept of the present invention. The present invention is applied to the rapid detection of the curve of the astaxanthin content in eggs changing with the exposure time.

[0051] Example 1: As Figure 1 shown, this embodiment provides a method for detecting the astaxanthin content in eggs, and the method includes the following steps:

[0052] S1, obtaining first data; the first data includes a preset measurement period, a preset first interval time, a preset first weight, a preset second weight, and a preset third weight.

[0053] Exemplarily, the measurement period is 600 seconds, the first interval time is 60 seconds, the first weight is 50 grams, the second weight is 40 grams, and the third weight is 10 grams.

[0054] S2, according to the preset number of sample groups M , equally dividing the pre-prepared sample egg liquid into the first grouped egg liquid to the M grouped egg liquid; calculating the mixing quantity M according to the number of sample groups k ; grouping and mixing the first grouped egg liquid to the M grouped egg liquid to obtain the first measured egg liquid to the k measured egg liquid; respectively extracting egg liquid with a weight of the first weight from the first measured egg liquid to the k measured egg liquid to obtain the first comparison egg liquid to the k comparison egg liquid; using the extraction method to extract astaxanthin from the first comparison egg liquid to the k comparison egg liquid to obtain the first comparison solution to the k comparison solution; the weights of the first comparison solution to the k comparison solution are all the third weight; respectively and evenly applying the first comparison solution to the k comparison solution onto the surface of the pre-set first white test paper to the k white test paper to obtain the first comparison sample to the k comparison sample.

[0055] Exemplarily, the preset number of sample groupsM = 100, the pre-prepared sample egg liquid is equally divided into the first group of egg liquid to the 100th group of egg liquid. The mixing quantity is calculated k = 13. The first group of egg liquid to the 100th group of egg liquid are grouped and mixed to obtain the first measured egg liquid to the 13th measured egg liquid. Egg liquid with a weight of 50 grams is extracted from the first measured egg liquid to the 13th measured egg liquid respectively to obtain the first comparison egg liquid to the 13th comparison egg liquid. The extraction method is used to extract astaxanthin from the first comparison egg liquid to the 13th comparison egg liquid to obtain the first comparison solution to the 13th comparison solution. The weights of the first comparison solution to the 13th comparison solution are all 10 grams. The extraction method is to add methanol-dichloromethane solution and sodium hydroxide-methanol solution to the first comparison egg liquid to the 13th comparison egg liquid in sequence to extract astaxanthin. The weight of the methanol-dichloromethane solution is the fourth weight set in advance. The concentration of the methanol-dichloromethane solution is the fourth concentration set in advance. The weight of the sodium hydroxide-methanol solution is the fifth weight set in advance. The concentration of the sodium hydroxide-methanol solution is the fifth concentration set in advance. The first comparison solution to the 13th comparison solution are evenly smeared on the surfaces of the first white test paper to the 13th white test paper respectively to obtain the first comparison sample to the 13th comparison sample.

[0056] S3, taking the first interval time as the time interval, using high performance liquid chromatography to measure the astaxanthin content of the first measured egg liquid to the k measured egg liquid during the measurement period, and fitting to obtain the first measured astaxanthin content curve to the k measured astaxanthin content curve; taking the first interval time as the time interval, collecting the images of the first comparison sample to the k comparison sample during the measurement period to obtain the first sample image group to the k sample image group.

[0057] Exemplarily, taking 60 seconds as the time interval, using high performance liquid chromatography to measure the astaxanthin content of the first measured egg liquid to the 13th measured egg liquid, and fitting to obtain the first measured astaxanthin content curve to the 13th measured astaxanthin content curve. The measurement time interval is 600 seconds. Astaxanthin will undergo a redox reaction when exposed to air, resulting in a gradual decrease in the astaxanthin content with the increase of exposure time. The first measured astaxanthin content curve to the 13th measured astaxanthin content curve respectively reflect the change relationship of the astaxanthin content in the first measured egg liquid to the 13th measured egg liquid with the exposure time. Through the camera installed directly above the first comparison sample to the 13th comparison sample, the images of the first comparison sample to the 13th comparison sample are collected at 60-second intervals to obtain the first sample image group to the 13th sample image group. The collection time interval is 600 seconds.

[0058] S4, extract egg liquid with a weight of the first weight from the egg liquid to be tested to obtain the first egg liquid to be tested; use an extraction method to extract astaxanthin from the first egg liquid to be tested to obtain the first solution to be tested; the weight of the first solution to be tested is the third weight; evenly apply the first solution to be tested on the surface of a pre-set test white test paper to obtain the first test sample; collect images of the first test sample at intervals of the first time interval to obtain the first group of test images; according to the first group of test images and the first group of sample images to the k group of sample images, use a color difference comparison algorithm to calculate the astaxanthin content curve of the egg liquid to be tested.

[0059] Exemplarily, extract 50 grams of egg liquid from the egg liquid to be tested to obtain the first egg liquid to be tested. Use an extraction method to extract astaxanthin from the first egg liquid to be tested to obtain 10 grams of the first solution to be tested. The extraction method is to add a methanol-dichloromethane solution and a sodium hydroxide-methanol solution to the first egg liquid to be tested in sequence to extract astaxanthin. The weight of the methanol-dichloromethane solution is the pre-set fourth weight. The concentration of the methanol-dichloromethane solution is the pre-set fourth concentration. The weight of the sodium hydroxide-methanol solution is the pre-set fifth weight. The concentration of the sodium hydroxide-methanol solution is the pre-set fifth concentration. Evenly apply the first solution to be tested on the surface of the test white test paper to obtain the first test sample. The sizes of the test white test paper, the first white test paper to the 13th white test paper are all the same. Collect images of the first test sample at intervals of 60 seconds through a camera installed directly above the first test sample to obtain the first group of test images. The acquisition time interval is 600 seconds. According to the first group of test images and the first group of sample images to the 13th group of sample images, use a color difference comparison algorithm to calculate the astaxanthin content curve of the egg liquid to be tested.

[0060] Further, the method for M calculating the mixing quantity obtained according to the number of sample groups k includes:

[0061] According to the number of sample groups M , use a mixing calculation formula to calculate the mixing quantity k ; the mixing calculation formula is:

[0062] ;

[0063] wherein, represents the floor function symbol.

[0064] Exemplarily, since M = 100, calculate to obtain .

[0065] Further, the method of obtaining the first measured egg liquid to the M measured egg liquid by grouping and mixing the first grouped egg liquid to the k grouped egg liquid includes:

[0066] Extracting astaxanthin from the first grouped egg liquid to the grouped egg liquid to obtain the first extraction solution to the extraction solution; the weights of the first extraction solution to the extraction solution are all the second weight; adding the extraction solution to the extraction solution to the grouped egg liquid, iterating through the integers between 2 and to obtain the second measured egg liquid to the k measured egg liquid; where and The calculation formula of is:

[0067] ;

[0068] Denote the grouped egg liquid as the first measured egg liquid.

[0069] Exemplarily, extract astaxanthin from the first grouped egg liquid to the 87th grouped egg liquid to obtain the first extraction solution to the 87th extraction solution. The weights of the first extraction solution to the 87th extraction solution are all 40 grams. When , calculate to obtain ,, so mix the first extraction solution with the 89th grouped egg liquid to obtain the second measured egg liquid. When , calculate to obtain ,, so mix the second extraction solution and the third extraction solution with the 90th grouped egg liquid to obtain the third measured egg liquid. And so on, obtain the second measured egg liquid to the 13th measured egg liquid. In addition, directly denote the 88th grouped egg liquid as the first measured egg liquid.

[0070] Further, at intervals of the first interval time, measure the astaxanthin content of the first measured egg liquid to the k measured egg liquid within the measurement period by liquid chromatography, and fit to obtain the first measured astaxanthin content curve to the k measured astaxanthin content curve, the method includes:

[0071] At intervals of the first interval time, measure the astaxanthin content of the first measured egg liquid to the kMeasure the astaxanthin content of the egg liquid to obtain the first measured astaxanthin content time series to the k Measured astaxanthin content time series; the first measured egg liquid to the k The unit for measuring the astaxanthin content of the egg liquid is milligrams per kilogram.

[0072] Use the least squares fitting algorithm to fit the first measured astaxanthin content time series to the k Measured astaxanthin content time series, and obtain the first measured astaxanthin content curve to the k Measured astaxanthin content curve; define the independent variable of the first measured astaxanthin content curve to the k Measured astaxanthin content curve as the astaxanthin exposure time .

[0073] Exemplarily, at 60 - second time intervals, use liquid chromatography to measure the astaxanthin content of the first measured egg liquid to the 13th measured egg liquid within 600 seconds, and obtain the first measured astaxanthin content time series to the 13th measured astaxanthin content time series; the unit for the astaxanthin content of the first measured egg liquid to the 13th measured egg liquid is milligrams per kilogram. Use the least squares fitting algorithm to fit the first measured astaxanthin content time series to the 13th measured astaxanthin content time series respectively, and obtain the first measured astaxanthin content curve to the 13th measured astaxanthin content curve. Define the independent variable of the first measured astaxanthin content curve to the 13th measured astaxanthin content curve as the astaxanthin exposure time . The value range of is from 0 to 600 seconds.

[0074] Furthermore, the method for calculating the astaxanthin content curve of the to - be - measured egg liquid according to the first group of to - be - measured images and the first group of sample images to the k Group of sample images includes:

[0075] Evenly divide each frame of image in the first group of to - be - measured images to obtain To - be - measured image segments; where Is the preset horizontal segmentation quantity, Is the preset vertical segmentation quantity, Is the sequence number; where The calculation formula of is:

[0076] ;

[0077] Among them, Represents the measurement period; Represents the first interval time.

[0078] Exemplarily, calculate to obtain Pre-set , , so each frame image in the first group of images to be measured is evenly divided to obtain image segments to be measured.

[0079] Label the image segments to be measured in sequence as ; where is an integer variable with a value ranging from 1 to , is an integer variable with a value ranging from 1 to , is an integer variable with a value ranging from 1 to .

[0080] Evenly divide each frame image in the first group of sample images to the k th group of sample images to obtain image segments; label the image segments in sequence as ; where is an integer variable with a value ranging from 1 to .

[0081] Exemplarily, evenly divide each frame image in the first group of sample images to the 13th group of sample images to obtain image segments.

[0082] Use a color difference meter to measure the tristimulus values of the image segments to be measured respectively to obtain , , ; use a color difference meter to measure the tristimulus values of the image segments respectively to obtain , , .

[0083] Match , , with , , to obtain the first similarity , the second similarity , the first matching serial number and the second matching serial number .

[0084] According to the first similarity, the second similarity, the first matching serial number, the second matching serial number, the first measured astaxanthin content curve to the kThe astaxanthin content curve of the egg liquid to be measured is calculated from the measured astaxanthin content curve.

[0085] Further, the step of 、 、 is matched with 、 、 to obtain the first similarity 、the second similarity 、the first matching serial number and the second matching serial number includes:

[0086] According to 、 、 is matched with 、 、 to calculate a trichromatic distance sequence; the calculation formula of the trichromatic distance sequence is:

[0087] ;

[0088] where represents the th element in the trichromatic distance sequence.

[0089] Exemplarily, it is calculated from to .

[0090] Search for the two smallest values in the trichromatic distance sequence, and denote them as 、 respectively; the element numbers corresponding to 、 in the trichromatic distance sequence are denoted as the first matching serial number and the second matching serial number respectively.

[0091] Exemplarily, to the two smallest values are and respectively, so the first matching serial number is 2 and the second matching serial number is 3.

[0092] According to 、 , the first similarity and the second similarity are calculated using a similarity algorithm.

[0093] Further, the method of calculating the first similarity and the second similarity according to 、 using a similarity algorithm includes:

[0094] According to , calculate the first similarity and the second similarity; the calculation formulas for the first similarity and the second similarity are as follows:

[0095] .

[0096] Furthermore, the method for calculating the astaxanthin content curve of the egg liquid to be measured according to the first similarity, the second similarity, the first matching serial number, the second matching serial number, the first measured astaxanthin content curve to the k measured astaxanthin content curve includes:

[0097] According to the first similarity, the second similarity, the first matching serial number, the second matching serial number, the first measured astaxanthin content curve to the k measured astaxanthin content curve, use the curve weighting formula to calculate the astaxanthin content curve of the egg liquid to be measured; the curve weighting formula is:

[0098] ;

[0099] wherein, represents the astaxanthin content curve of the egg liquid to be measured, represents the measured astaxanthin content curve, represents the measured astaxanthin content curve.

[0100] Finally, it should be noted that: Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for detecting the astaxanthin content in eggs, characterized in that, The method includes the following steps: S1. Obtain first data; the first data includes a preset measurement period, a preset first interval time, a preset first weight, a preset second weight, and a preset third weight; S2. According to the preset number of sample groups M , equally divide the pre-prepared sample egg liquid into the first-group egg liquid to the M -group egg liquid; Calculate the mixing quantity M according to the preset number of sample groups k ; Mix the first-group egg liquid to the M -group egg liquid in groups to obtain the first measured egg liquid to the k measured egg liquid; Extract egg liquid with a weight of the first weight from the first measured egg liquid to the k measured egg liquid respectively to obtain the first comparison egg liquid to the k comparison egg liquid; Extract astaxanthin from the first comparison egg liquid to the k comparison egg liquid by an extraction method to obtain the first comparison solution to the k comparison solution; The weights of the first comparison solution to the k comparison solution are all the third weight; Coat the first comparison solution to the k comparison solution evenly on the surface of the pre-set first white test paper to the k white test paper respectively to obtain the first comparison sample to the k comparison sample; S3, at the first interval time as the time interval, within the measurement period, measure the first measured egg liquid to the k astaxanthin content of the measured egg liquid, and fit to obtain the first measured astaxanthin content curve to the k astaxanthin content curve; at the first interval time as the time interval, within the measurement period, collect the first comparison sample to the k comparison sample image, and obtain the first sample image group to the k sample image group; S4, extract egg liquid with a weight of the first weight from the egg liquid to be tested to obtain the first egg liquid to be tested; use an extraction method to extract astaxanthin from the first egg liquid to be tested to obtain the first solution to be tested; the weight of the first solution to be tested is the third weight; evenly apply the first solution to be tested on the surface of a pre-set test white test paper to obtain the first sample to be tested; collect images of the first sample to be tested at intervals of the first time interval to obtain the first group of images to be tested; according to the first group of images to be tested and the first group of sample images to the k group of sample images, use a color difference comparison algorithm to calculate the astaxanthin content curve of the egg liquid to be tested.

2. The method for detecting the astaxanthin content in eggs according to claim 1, wherein The said according to the number of sample groups M Calculating the mixing quantity k The method includes: According to the number of sample groups M , a mixed quantity is calculated by using a mixed calculation formula k ; the mixed calculation formula is as follows: ； Among them, represents the floor symbol.

3. The method for detecting the astaxanthin content in eggs according to claim 2, characterized in that, The method of mixing the first group of egg liquids to the M group of egg liquids to obtain the first measured egg liquid to the k method for measuring egg liquid includes: Extract the first group of egg liquid to the astaxanthin in the group of egg liquid to obtain the first extraction solution to the extraction solution; the weights of the first extraction solution to the extraction solution are all the second weight; combine the extraction solution to the extraction solution with the group of egg liquid, iterate through the integers between 2 and to obtain the second measured egg liquid to the k measured egg liquid; where and The calculation formula of is: ； Designate the grouped egg liquid as the first measured egg liquid.

4. The method for detecting the astaxanthin content in eggs according to claim 3, wherein Taking the first interval time as the time interval, within the measurement period, the liquid chromatography method is used to measure the first measured egg liquid to the k astaxanthin content of the measured egg liquid, and fitting to obtain the first measured astaxanthin content curve to the k The method for measuring the astaxanthin content curve includes: Taking the first interval time as the time interval, within the measurement period, the liquid chromatography method is used to measure the first measured egg liquid to the k astaxanthin content of the measured egg liquid, obtaining the first measured astaxanthin content time series to the k measured astaxanthin content time series; the first measured egg liquid to the k unit for measuring the astaxanthin content of the egg liquid is milligrams per kilogram; Using the least squares fitting algorithm, respectively fit the first measured astaxanthin content time series to the k measured astaxanthin content time series to obtain the first measured astaxanthin content curve to the k measured astaxanthin content curve; Define the independent variables of the first measured astaxanthin content curve to the k measured astaxanthin content curve as the astaxanthin exposure time .

5. The method for detecting the astaxanthin content in eggs according to claim 4, wherein, The method for calculating the astaxanthin content curve of the egg liquid to be measured according to the first image group to be measured and the first to k the first sample image group by using the color difference comparison algorithm includes: Uniformly divide each frame image in the first group of images to be measured to obtain image segments to be measured; where is the preset horizontal segmentation quantity, is the preset vertical segmentation quantity, is the sequence number; where The calculation formula of is: ； Among them, represents the measurement period; represents the first interval time; Label image segments to be measured in sequence as ; where is an integer variable with values ranging from 1 to , is an integer variable with values ranging from 1 to , is an integer variable with values ranging from 1 to ; Divide each frame image in the first sample image group to the k sample image group evenly to obtain sample image segments; Mark the sample image segments in sequence as ; where is an integer variable taking values from 1 to Measure the tristimulus values of image segments to be measured respectively using a color difference meter, obtaining , , ; Measure the tristimulus values of sample image segments respectively using a color difference meter, obtaining , , ; Match , , with , , to obtain the first similarity , the second similarity , the first matching serial number and the second matching serial number ;​​​​​​​​​​​​​​​​​​​​ Based on the first similarity, the second similarity, the first matching serial number, the second matching serial number, the first measured astaxanthin content curve to the k Measured astaxanthin content curve, the astaxanthin content curve of the egg liquid to be measured is calculated.

6. The method for detecting the astaxanthin content in eggs according to claim 5, characterized in that The method of matching , , with , , to obtain the first similarity , the second similarity , the first matching serial number and the second matching serial number comprises: According to , , and , , a trichromatic distance sequence is calculated; the calculation formula of the trichromatic distance sequence is: ； Among them represents the th element in the tristimulus distance sequence; Search for the two smallest values in the tristimulus distance sequence, denoted as , ; Denote the element numbers corresponding to , in the tristimulus distance sequence as the first matching serial number and the second matching serial number respectively; According to and , the first similarity and the second similarity are calculated using a similarity algorithm.

7. The method for detecting the astaxanthin content in eggs according to claim 6, wherein, The method according to , , for calculating the first similarity and the second similarity by using a similarity algorithm, includes: According to and calculate the first similarity and the second similarity; the calculation formulas for the first similarity and the second similarity are as follows: 。 8. The method for detecting the astaxanthin content in eggs according to claim 7, characterized in that, The method for calculating the astaxanthin content curve of the egg liquid to be measured according to the first similarity, the second similarity, the first matching serial number, the second matching serial number, the first measured astaxanthin content curve to the k nth measured astaxanthin content curve includes: According to the first similarity, the second similarity, the first matching serial number, the second matching serial number, the first measured astaxanthin content curve to the k measured astaxanthin content curve, the astaxanthin content curve of the egg liquid to be measured is calculated by using a curve weighting formula; the curve weighting formula is: ； Among them, represents the astaxanthin content curve of the egg liquid to be measured, represents the astaxanthin content measurement curve, represents the astaxanthin content measurement curve.

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