Immersion microscope and objective lens thereof

Abstract

The invention relates to an immersion microscope and an objective lens thereof. The objective lens comprises a first doublet lens, a first biconvex lens, a meniscus lens, a second biconvex lens, a second doublet lens, a third doublet lens and a plano-convex lens which are sequentially arranged from an image plane to an object plane. Wherein the second doublet lens is a variable refractive index group; the numerical aperture of the objective lens is greater than 0.6; the value range of the wavelength of the objective lens is [380 nm, 1550 nm]. According to the invention, the refractive index range of the immersion liquid matched with the immersion microscope can be widened.

Description

Technical Field

[0001] The present application relates to the field of microscope technology, and more specifically, to an immersion microscope and an objective lens thereof. Background Art

[0002] In the field of optical microscopy, immersion microscopy has been widely used as an important high-resolution imaging tool. The resolution of traditional air-medium microscopes is limited by the Abbe diffraction limit. Immersion microscopy breaks through this limitation by replacing the medium between the observed object and the microscope objective with an immersion liquid with a high refractive index, which effectively shortens the wavelength and significantly improves the resolution of the microscope. However, although the objective of the immersion microscope has shown great potential in improving resolution, it still faces a significant challenge in practical applications: the range of refractive index of the immersion liquid is not wide enough.

[0003] Specifically, different experiments may require the use of immersion fluids with different refractive indices to achieve the best imaging results. For example, in biological sample imaging, the refractive index of the sample may vary depending on the cell type, cell state, or treatment method. In addition, with the rapid development of nanotechnology and material science, more and more new materials are being used in scientific research, and the refractive index of these materials may also exceed the adaptability range of the objectives of traditional immersion microscopes.

[0004] Therefore, the objectives of current immersion microscopes have certain limitations in adapting the refractive index of the immersion liquid, which limits their application in a wider range of experimental conditions. To overcome this challenge, it is necessary to develop an immersion microscope with a wider refractive index adaptation range to meet the needs of different experimental conditions and sample types. Summary of the invention

[0005] The present application is provided to solve the above problems existing in the prior art. According to an immersion microscope and an objective lens thereof in an embodiment of the present application, the refractive index range of the immersion liquid adapted to the immersion microscope can be widened.

[0006] In a first aspect, an embodiment of the present application provides an objective lens of an immersion microscope, comprising a first doublet lens, a first biconvex lens, a meniscus lens, a second biconvex lens, a second doublet lens, a third doublet lens, and a plano-convex lens, which are arranged in sequence from an image plane to an object plane;

[0007] Among them, the second double-cemented lens is a variable refractive index group; the numerical aperture of the objective lens is greater than 0.6; the wavelength range of the objective lens is [380nm, 1550nm].

[0008] Optionally, the portion where the plane of the plano-convex lens contacts the mechanical part is coated with fluororubber, and the portion where the side of the plano-convex lens contacts the rubber ring is coated with silicone rubber.

[0009] Optionally, the objective lens further comprises: a rubber ring; the rubber ring is arranged on the edge of the plano-convex lens.

[0010] Optionally, from the image plane to the object plane, the spacing range between two adjacent mirror planes is [5.2mm-11.2mm], [1.73mm-7.73mm], [1.85mm-6.85mm], [4.1mm-11.1mm], [0mm-4.2mm], [3.8mm-9.8mm], [0mm-5.43mm], [3.1mm-9.1mm], [0.6mm-6.6mm], [0mm-4.4mm], [3mm-9mm], [0mm-5.56mm], [0mm-6mm], [1mm-7mm], [0mm-3.52mm], and [3mm-9mm], and the spacing range between the mirror plane of the plano-convex lens close to the object plane and the object plane is [5mm-11mm].

[0011] Optionally, from the image plane to the object plane, the curvature ranges of the mirror surfaces are [-12.65, -6.65], [-9.82, -3.82], [10.57, 16.57], [41.85, 47.85], [-79.552, -73.552], [-19.5, -13.5], [-23.637, -17.637], [2 9.9,35.9], [-51.5,-45.5], [16.58,22.58], [9.1,15.1], [-86.85,-80.85], [11.052,17.052], [-29.431,-23.431], [5.5,11.5], [5.755,11.755], [-3,3].

[0012] Optionally, the numerical aperture of the objective lens is 0.63.

[0013] Optionally, the field of view of the objective lens is 18 mm.

[0014] Optionally, the parfocal distance of the objective lens is 75 mm.

[0015] Optionally, the rear focal plane position of the objective lens is -48.1 mm.

[0016] In a second aspect, an embodiment of the present application provides an immersion microscope, comprising the objective lens described in any of the above embodiments.

[0017] The beneficial effects of the embodiments of the present application are that the objective lens is composed of a first double-cemented lens, a first double convex lens, a meniscus lens, a second double convex lens, a second double-cemented lens, a third double-cemented lens and a plano-convex lens arranged in sequence, and the refractive index range of the liquid adapted by the lens group composed of these lenses can reach 1.3-1.6, compared with the existing refractive index range of 1.52-1.58, which can greatly broaden the application scenarios of immersion microscopes. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In the drawings, which are not necessarily drawn to scale, the same reference numerals may describe similar parts in different views. The same reference numerals with letter suffixes or different letter suffixes may represent different instances of similar parts. The accompanying drawings generally illustrate various embodiments by way of example and not limitation, and together with the specification and claims, are used to illustrate the disclosed embodiments. When appropriate, the same reference numerals are used throughout the drawings to refer to the same or similar parts. Such embodiments are illustrative and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.

[0019] Figure 1 It is a schematic diagram of the structure of an objective lens of an immersion microscope provided in one embodiment of the present application. DETAILED DESCRIPTION

[0020] In order to enable those skilled in the art to better understand the technical solution of the present application, the present application is described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present application are further described in detail below in conjunction with the accompanying drawings and specific embodiments, but are not intended to limit the present application. The terms "first", "second" and "third" used in the present application are only intended to distinguish the corresponding features, do not represent the need for such an order, and do not necessarily represent only the singular form.

[0021] like Figure 1 As shown, an embodiment of the present application provides an objective lens of an immersion microscope, including a first double-cemented lens (including 100 and 200), a first double convex lens 300, a meniscus lens 400, a second double convex lens 500, a second double-cemented lens (including 600 and 700), a third double-cemented lens (including 800 and 900) and a plano-convex lens 1000 arranged in sequence from the image plane to the object plane.

[0022] Among them, the second double-cemented lens is a variable refractive index group; the numerical aperture of the objective lens is greater than 0.6; the wavelength range of the objective lens is [380nm, 1550nm], such as 380nm, 1550nm or 1000nm.

[0023] The refractive index range of the liquid adapted by the lens group composed of these 10 lenses can reach 1.3-1.6. Compared with the existing refractive index range of 1.52-1.58, it can greatly broaden the application scenarios of immersion microscopes, and the objective lens has a lower cost.

[0024] In one embodiment of the present application, from the image plane to the object plane, the spacing ranges between two adjacent mirror planes are [5.2mm-11.2mm], [1.73mm-7.73mm], [1.85mm-6.85mm], [4.1mm-11.1mm], [0mm-4.2mm], [3.8mm-9.8mm], [0mm-5.43mm], [3.1mm-9.1mm], [0.6mm-6.6mm], [0mm-4.4mm], [3mm-9mm], [0mm-5.56mm], [0mm-6mm], [1mm-7mm], [0mm-3.52mm], and [3mm-9mm], and the spacing range between the mirror plane of the plano-convex lens close to the object plane and the object plane is [5mm-11mm].

[0025] For example, the thickness of lens 100 in the first double-cemented lens is [5.2 mm-11.2 mm], so the distance between the first mirror surface and the second mirror surface is [5.2 mm-11.2 mm] of the thickness of lens 100, the thickness of lens 200 is [1.73 mm-7.73 mm], so the distance between the second mirror surface and the third mirror surface is [1.73 mm-7.73 mm], and so on. The embodiment of the present application widens the range of the refractive index of the adapted liquid by adjusting the thickness of the lens and the distance between the lenses.

[0026] In one embodiment of the present application, from the image plane to the object plane, the spacing between two adjacent mirror surfaces is 8.2mm, 4.73mm, 3.85mm, 7.1mm, 1.2mm, 6.8mm, 2.43mm, 6.1mm, 3.6mm, 1.4mm, 6mm, 2.56mm, 3mm, 4mm, 0.52mm, 6mm, and the spacing between the mirror surface of the plano-convex lens close to the object plane and the object plane is 8mm. The objective lens provided in the embodiment of the present application has a wide refractive index range.

[0027] In one embodiment of the present application, from the image plane to the object plane, the curvature ranges of the mirror surfaces are [-12.65, -6.65], [-9.82, -3.82], [10.57, 16.57], [41.85, 47.85], [-79.552, -73.552], [-19.5, -13.5], [-23.637, -17.637], respectively. ]、[29.9,35.9]、[-51.5,-45.5]、[16.58,22.58]、[9.1,15.1]、[-86.85,-80.85]、[11.052,17.052]、[-29.431,-23.431]、[5.5,11.5]、[5.755,11.755]、[-3,3].

[0028] For example, the curvature range of one mirror surface of lens 100 is [-12.65, -6.65], the curvature range of the mirror surface shared by lens 100 and lens 200 is [-9.82, -3.82], and the curvature range of another mirror surface of lens 200 is [10.57, 16.57].

[0029] In one embodiment of the present application, from the image plane to the object plane, the curvatures of the mirror surfaces are -9.65, -6.82, 13.57, 44.85, -76.552, -16.5, -20.637, 32.9, -48.5, 19.58, 12.1, -83.85, 14.052, -26.431, 8.5, 8.755, 0. The curvature provided in the embodiment of the present application is a preferred case within the curvature range, which enables the objective lens to adapt to a wider range of refractive indices.

[0030] In one embodiment of the present application, the numerical aperture of the objective lens is 0.63.

[0031] In one embodiment of the present application, the field of view of the objective lens is not greater than 18 mm, preferably 18 mm.

[0032] In one embodiment of the present application, the parfocal distance of the objective lens is 75 mm. In actual application scenarios, the parfocal distance can also be adjusted according to actual needs.

[0033] In one embodiment of the present application, the back focal plane position of the objective lens is -48.1 mm. In actual application scenarios, the parfocal distance and the back focal plane position can also be adjusted according to actual needs.

[0034] In one embodiment of the present application, the materials of each lens in the objective lens are shown in Table 1. The selection of these materials can achieve a wider range of refractive indexes more accurately and at a low cost. In actual application scenarios, the materials of each lens can also be replaced with glass or the like.

[0035] Table 1

[0036]

[0037]

[0038] In one embodiment of the present application, the objective lens further comprises a rubber ring; the rubber ring is arranged at the edge of the plano-convex lens. In order to further enhance the water-repellent and corrosion-resistant performance of the objective lens, the present embodiment uses a rubber ring. In practical application scenarios, the rubber ring can also be used in combination with silicone rubber.

[0039] In one embodiment of the present application, the plane of the plano-convex lens is coated with fluororubber on the part in contact with the mechanical part, and the side of the plano-convex lens is coated with silicone rubber on the part in contact with the rubber ring. Considering that some immersion liquids are corrosive and easily damage the microscope structure, causing liquid to penetrate and affect the use of the equipment, for this reason, the present application applies fluororubber on the part in contact with the plane of the plano-convex lens 1000 and the mechanical part, and applies silicone rubber on the part in contact with the rubber ring on the side of the plano-convex lens to enhance its waterproof and corrosion resistance. The immersion liquid includes benzyl benzoate, benzyl alcohol, etc. Fluororubber can also be replaced by polytetrafluoroethylene.

[0040] The embodiment of the present application provides an immersion microscope, comprising the objective lens described in any of the above embodiments. The refractive index range of the liquid adapted for the 10X immersion microscope is 1.3-1.6.

[0041] During use, the compensation ring of the immersion microscope can be adjusted to change the liquid Figure 1 The distance of floating 1 in the middle can be adjusted to adapt the refractive index of the immersion liquid, so that the 10X immersion microscope can adapt to more scenes.

[0042] Furthermore, although exemplary embodiments have been described herein, the scope thereof includes any and all embodiments based on the present application having equivalent elements, modifications, omissions, combinations (eg, schemes that cross various embodiments), adaptations, or changes.

[0043] The elements in the claims are to be interpreted broadly based on the language employed in the claims and are not limited to the examples described in this specification or during the prosecution of the application, which examples are to be interpreted as non-exclusive. Therefore, this specification and examples are intended to be considered as examples only, with the true scope and spirit being indicated by the claims and the full scope of their equivalents.

[0044] The order of each step in the present application is merely exemplary and not restrictive. Without affecting the implementation of the present application (without destroying the required logical relationship between the steps), the execution order of the steps can be adjusted, and the various embodiments obtained after the adjustment still fall within the scope of the present application.

[0045] The above description is intended to be illustrative rather than restrictive. For example, the above examples (or one or more of them) can be used in combination with each other. For example, those of ordinary skill in the art can use other embodiments when reading the above description. In addition, in the above-mentioned specific embodiments, various features can be grouped together to simplify the application. This should not be interpreted as an intention that a disclosed feature that does not require protection is necessary for any claim. On the contrary, the subject matter of the present invention may be less than all the features of a specific disclosed embodiment. Thus, the claims are incorporated into the specific embodiments as examples or embodiments, wherein each claim is independently used as a separate embodiment, and it is considered that these embodiments can be combined with each other in various combinations or arrangements. The scope of the present invention should be determined with reference to the attached claims and the full scope of equivalent forms granted by these claims.

Claims

1. An objective lens for an immersion microscope, characterized in that: It includes a first double cemented lens, a first double convex lens, a meniscus lens, a second double convex lens, a second double cemented lens, a third double cemented lens and a plano-convex lens which are arranged in sequence from the image plane to the object plane; Among them, the second double-cemented lens is a variable refractive index group; the numerical aperture of the objective lens is greater than 0.6; the wavelength range of the objective lens is [380nm, 1550nm].

2. The objective lens according to claim 1, characterized in that Further including: Rubber ring; The rubber ring is arranged on the edge of the plano-convex lens.

3. The objective lens according to claim 2, characterized in that The contact portion between the plane of the plano-convex lens and the mechanical part is coated with fluororubber, and the contact portion between the side surface of the plano-convex lens and the rubber ring is coated with silicone rubber.

4. The objective lens according to claim 1, characterized in that From the image plane to the object plane, the spacing ranges of two adjacent mirror surfaces are [5.2mm-11.2mm], [1.73mm-7.73mm], [1.85mm-6.85mm], [4.1mm-11.1mm], [0mm-4.2mm], [3.8mm-9.8mm], [0mm-5.43mm], [3.1mm-9.1mm], [0.6mm-6.6mm], [0mm-4.4mm], [3mm-9mm], [0mm-5.56mm], [0mm-6mm], [1mm-7mm], [0mm-3.52mm], and [3mm-9mm] respectively, and the spacing range between the mirror surface of the plano-convex lens close to the object plane and the object plane is [5mm-11mm].

5. The objective lens according to claim 1, characterized in that From the image plane to the object plane, the curvature ranges of each mirror surface are [-12.65, -6.65], [-9.82, -3.82], [10.57, 16.57], [41.85, 47.85], [-79.552, -73.552], [-19.5, -13.5], [-23.637, -17.637], [29. 9,35.9], [-51.5,-45.5], [16.58,22.58], [9.1,15.1], [-86.85,-80.85], [11.052,17.052], [-29.431,-23.431], [5.5,11.5], [5.755,11.755], [-3,3].

6. The objective lens according to claim 1, characterized in that The numerical aperture of the objective lens is 0.

63.

7. The objective lens according to claim 1, characterized in that The field number of the objective lens is 18 mm.

8. The objective lens according to claim 1, characterized in that The parfocal distance of the objective lens is 75 mm.

9. The objective lens according to claim 1, characterized in that The back focal plane position of the objective lens is -48.1 mm.

10. An immersion microscope, characterized in that: The objective lens comprises the objective lens described in any one of claims 1 to 9.

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