Protein, polynucleotide, expression vector, transformant, composition containing plasmalogen and preparation method of plasmalogen

By introducing the acetal phospholipid synthase gene from anaerobic bacteria into aerobic bacteria, the acetal phospholipids are synthesized under aerobic conditions, solving the complex problems of culture under anaerobic conditions in the prior art, simplifying the process and improving production efficiency.

CN119731325APending Publication Date: 2025-03-28FOOD RHEOLOGY RES INST CO LTD +1
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Patent Information

Application Number
CN202380059633.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2022-09-02
Filing Date
2023-08-21
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The prior art is difficult to cultivate bacteria on a large scale under aerobic conditions to synthesize acetal phospholipids, and culture under anaerobic conditions is complex and difficult to maintain.

Method used

The method of synthesizing acetal phospholipids under aerobic conditions is realized by introducing the anaerobic bacterial acetal phospholipid synthase gene from anaerobic bacteria.

Benefits of technology

Successfully biosynthesis of acetal phospholipids from bacteria under aerobic conditions simplifies the culture process and reduces cost and complexity.

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Abstract

A protein comprising an amino acid sequence selected from the group consisting of (a), (b), (c), and (d) described below, and having a plasmalogen synthesis activity in a bacterium expressing the protein, (a) an amino acid sequence as shown in SEQ ID NO.1, (b) an amino acid sequence with more than 80% of sequence identity relative to the amino acid sequence as shown in SEQ ID NO.1, (c) an amino acid sequence as shown in SEQ ID NO.2, and (d) an amino acid sequence with more than 80% of sequence identity relative to the amino acid sequence as shown in SEQ ID NO.2.
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