Method for promoting growth of hermetia illucens and promoting protein synthesis of hermetia illucens bodies by utilizing ultraviolet induction

Abstract

The invention discloses a method for promoting hermetia illucens to grow and promoting hermetia illucens to synthesize protein through ultraviolet induction, and belongs to the field of resource utilization of urban organic solid waste. According to the method, ultraviolet lamps are mainly adopted to irradiate the hermetia illucens before the hermetia illucens are inoculated with the substrate, after the hermetia illucens are fed for 4 days, 8 days and 10 days, utilization of the substrate by hermetia illucens bodies is promoted, more biomass is accumulated, and the utilization value of the hermetia illucens bodies is improved. According to the method disclosed by the invention, the absorption and conversion capability of the hermetia illucens bodies on nutrient substances in the substrate is improved by utilizing ultraviolet induction, so that the growth of the hermetia illucens is further promoted, meanwhile, the uptake capability of the hermetia illucens bodies on the nutrient substances in the substrate is improved, and the reduction performance of the hermetia illucens on organic solid wastes in cities and towns is enhanced. The hermetia illucens bodies are irradiated by the ultraviolet lamp before inoculation and after the hermetia illucens enter the pre-pupa stage after being fed for 10 days, so that protein synthesis of the hermetia illucens bodies can be effectively promoted, and the content of the synthesized protein is up to 34.2%.

Description

Technical Field

[0001] The invention relates to the field of resource utilization of urban organic solid waste, and in particular to a method for promoting the growth of black soldier flies and promoting protein synthesis of insect bodies by utilizing ultraviolet induction. Background Art

[0002] As people's living standards continue to improve, garbage from households, catering, commercial activities and municipal services has led to an increasing amount of urban organic solid waste. Urban organic solid waste has a complex composition, high water content, is easy to rot and stink, and has a large volume and low density. But at the same time, it has a high organic matter content and obvious high-value resource characteristics. Although it can be effectively utilized through anaerobic digestion and aerobic composting to achieve the purpose of resource utilization, this process usually has a long cycle, and the treatment process is prone to produce foul-smelling gases, which poses an environmental risk.

[0003] Compared with traditional technologies such as anaerobic fermentation and aerobic composting, insect fermentation has the advantages of high cost-effectiveness, easy operation, low maintenance requirements, and low environmental impact, and is considered to be an economically viable alternative. Among them, black soldier flies are considered to be one of the most promising species for large-scale production due to their diverse diets, large food intake, and ability to efficiently convert organic waste into high-value products such as protein and oil. Many organic wastes, such as agricultural and forestry wastes, livestock and poultry manure, kitchen waste, and distiller's grains, can be effectively converted by black soldier fly larvae. However, the diversity of the nature of organic waste and the variability of nutritional components will lead to a decrease in the efficiency of black soldier fly larvae in treating organic waste. Although some current methods, such as mixing or pre-treating the substrate, can promote the utilization of organic waste by black soldier fly larvae, these methods require multi-faceted consideration and attempts on the composition, ratio, pre-treatment method, and substrate state of the mixed organic solid waste.

[0004] In addition, insect protein has attracted much attention due to its high-quality nutritional content and highly sustainable production methods. Insect protein is rich in high-quality amino acids and trace elements, and is considered to be one of the important sources for the future food and feed industry. The black soldier fly is an insect of the genus Soldierfly in the family Smilodon in the order Diptera. It has the characteristics of high food conversion rate, short breeding cycle, high content of fat, protein, minerals and vitamins. The protein content of black soldier fly larvae harvested by current breeding technology can reach between 10% and 40% of the insect weight, and there is still much room for improvement.

[0005] Therefore, if some inducing measures can be taken directly on the black soldier fly larvae themselves to promote the growth of black soldier flies and at the same time promote the synthesis of protein in the black soldier fly body, it will help to more directly and simply achieve the purpose of improving the efficiency of organic waste treatment and promoting the synthesis of protein in the body. Summary of the invention

[0006] In view of the above problems, the purpose of the present invention is to provide a method for promoting the growth of black soldier flies and promoting the synthesis of protein in the insect body by utilizing ultraviolet induction. By combining ultraviolet induction in the process of breeding black soldier flies to promote the growth of black soldier flies, the absorption and conversion capacity of nutrients in the substrate is increased, thereby improving the consumption and treatment efficiency of organic waste, and promoting the synthesis of protein, so as to solve the problems of the existing black soldier flies' reduced treatment efficiency of organic waste and the limited ability of the black soldier flies to synthesize protein.

[0007] To achieve the above-mentioned purpose, the present invention provides a method for promoting the growth of black soldier flies and promoting protein synthesis by using ultraviolet induction. The method uses ultraviolet induction to stimulate black soldier flies larvae to improve the feeding behavior and metabolic function of the larvae to promote the biomass accumulation of black soldier flies and protein synthesis, and comprises the following steps:

[0008] (1) placing black soldier fly eggs in a constant temperature and humidity incubator for hatching to obtain black soldier fly larvae;

[0009] (2) raising the hatched black soldier fly larvae in wheat bran with a moisture content of 60% for 8 days;

[0010] (3) inoculating 8-day-old black soldier fly larvae into feed for feeding;

[0011] (4) Before larval inoculation and / or during feeding, a UV light with a power of 8 W, a wavelength of 254 nm, and a UV dose of 46 μW·s / cm 2 The ultraviolet light is used for irradiation treatment;

[0012] (5) After 12 days of rearing, separate the substrate and black soldier flies.

[0013] In one embodiment of the present invention, the incubation environment in step (1) is a constant temperature and humidity incubator with an ambient temperature of 25-30° C. and a relative humidity of 60-70%.

[0014] In one embodiment of the present invention, the moisture content of the feed in step (3) is 70%, the composition ratio is soybean meal: wheat bran: corn flour 1:1:1, and the dry matter weight is 100g.

[0015] In one embodiment of the present invention, the inoculation ratio of the black soldier fly larvae in step (3) is 3 to 4 black soldier fly larvae per 1 to 2 g of dry matter feed.

[0016] In one embodiment of the present invention, the raising in step (3) is carried out in an environment with a temperature of 30° C. and a relative humidity of 70%.

[0017] In one embodiment of the present invention, in step (4), ultraviolet light irradiation is performed before larvae inoculation, and after ultraviolet light irradiation, the black soldier fly larvae are inoculated into feed for breeding.

[0018] In one embodiment of the present invention, in step (4), ultraviolet light irradiation is performed during larval rearing.

[0019] In one embodiment of the present invention, in step (4), the ultraviolet light irradiation is performed after the larvae have been reared for 4 days or 8 days.

[0020] In one embodiment of the present invention, in step (4), ultraviolet light irradiation is performed before larvae inoculation and during feeding.

[0021] In one embodiment of the present invention, in step (4), ultraviolet light irradiation is performed before larvae inoculation, after larvae are reared for 4 days, and after larvae are reared for 8 days.

[0022] In one embodiment of the present invention, in step (4), ultraviolet light irradiation is performed after 10 days of rearing. The black soldier fly larvae have entered the prepupal stage on the 10th day of rearing, and irradiation after 10 days of rearing is actually irradiation treatment of the black soldier flies in the prepupal stage.

[0023] In one embodiment of the present invention, in step (4), after irradiation with ultraviolet light after 10 days of feeding, the larvae in the prepupal stage are placed in new feed and continue to be fed for 2 days.

[0024] In one embodiment of the present invention, the ultraviolet light irradiation time in step (4) is 5 to 30 minutes.

[0025] In one embodiment of the present invention, an 80-mesh sieve is used to separate the black soldier flies and the substrate in step (5).

[0026] In one embodiment of the present invention, after step (5), the harvested black soldier flies and substrate are used for component analysis.

[0027] The invention also discloses a black soldier fly obtained according to the method.

[0028] The invention also discloses an application of the black soldier fly in the field of treating organic waste.

[0029] Beneficial effects:

[0030] (1) Ultraviolet induction was used to improve the black soldier fly's ability to absorb and transform nutrients in the substrate, thereby promoting the growth of the black soldier fly, resulting in an increase in the black soldier fly's body weight of up to 10.64%.

[0031] (2) Ultraviolet induction was used to improve the black soldier fly's ability to absorb nutrients from the substrate, thereby enhancing its ability to reduce urban organic solid waste.

[0032] (3) The irradiation treatment of black soldier flies by the method of the present invention helps the black soldier flies to convert the ingested nutrients into body proteins, thereby increasing the ability of the black soldier flies to synthesize protein. After 8-day-old black soldier flies were raised in feed for 10 days and then irradiated with ultraviolet light for 30 minutes, the amino acid content in the black soldier flies increased by 21.9% compared with the amino acid content in the black soldier flies that were not irradiated with ultraviolet light, and the protein content was as high as 34.2%. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 A schematic diagram of the process used in the method of promoting the growth of black soldier flies and promoting protein synthesis in the insect body by ultraviolet induction of the present invention;

[0034] Figure 2 It is a conventional nutritional index diagram of black soldier fly larvae in Examples 1 to 8 and Comparative Example 1;

[0035] Figure 3 is the substrate dry matter reduction rate. DETAILED DESCRIPTION

[0036] The following is a detailed description of the specific embodiments of the present invention. It should be understood that the embodiments are intended to better explain the present invention, but are not limited to the present invention. If no specific conditions are specified in the embodiments, the conditions are carried out according to conventional conditions or manufacturer recommendations. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0037] Black soldier fly amino acid test method:

[0038] 1. Weighing: Take the hydrolysis tube and weigh about 100.0 mg of sample, accurate to one decimal place.

[0039] 2. Add acid: add 8mL 6M HCL. (First add 4mL and shake to moisten, then add the rest of the HCL)

[0040] 3. Fill with nitrogen and seal the tube: Fill with nitrogen for 3 minutes, adjust the flow rate to make the solution slightly boiling, and tighten the hydrolysis tube cap.

[0041] 4. Hydrolysis: Place in an oven at 120°C and hydrolyze for 22-24 hours.

[0042] 5. Neutralization: Transfer all the samples in the hydrolysis tube to a stoppered test tube or volumetric flask, add 4.8mL 10M NaOH for neutralization, and dilute to 25mL with distilled water. If it exceeds the scale line, dilute to 50mL and record the dilute volume.

[0043] 6. Filtration: Use double-layer filter paper for filtration

[0044] 7. Centrifugation: Take 1 mL of clarified filtrate into a 1.5 mL centrifuge tube and centrifuge at 10,000 rpm for 10 min.

[0045] 8. Test: Take 400 μL of supernatant into a liquid sample bottle and test using an amino acid analyzer.

[0046] Testing method for nutritional index of black soldier flies:

[0047] (1) Ash content determination method:

[0048] Accurately weigh 2 g of the dried sample, put it into a crucible, and burn it in a muffle furnace at 550° C. to constant weight. The ash content is measured according to the formula: ash content = (initial mass - mass after burning) / initial mass.

[0049] (2) Protein determination method:

[0050] Accurately weigh 0.5 of the dried sample and place it in a dry digestion tube. Add 0.2g of copper sulfate, 3g of potassium sulfate and 10mL of concentrated sulfuric acid, and digest it in a digestion furnace. No sample is added to the blank sample. The digestion temperature is set to 120℃, 30 minutes, 240℃, 40 minutes, 320℃, 40 minutes, 380℃, 30 minutes, and then 420℃, 1.5 hours. Then use a Kjeldahl nitrogen analyzer for distillation; after the sample is distilled, add 2 drops of indicator (2% boric acid: methyl red: bromocresol green = 100: 1 / 6: 5 / 6), titrate the sample with 0.1mol of hydrochloric acid standard solution until it turns red, boil for 2 minutes, cool, and titrate again until it turns red. When the solution color is still red after heating and cooling, the titration endpoint is determined.

[0051] (3) Fat determination method:

[0052] Weigh 2 g of the dried sample accurately, wrap it in a filter paper box, and place it in a Soxhlet extractor. Extract the dried sample in a 60°C water bath with petroleum ether as solvent for 12 h. Dry the flask containing the fat and weigh it.

[0053] Example 1

[0054] A method for promoting the growth of black soldier flies by ultraviolet induction comprises the following steps:

[0055] (1) Mix soybean meal, wheat bran and corn flour in a ratio of 1:1:1 as a substrate for feeding black soldier fly larvae;

[0056] (2) placing 20 g of black soldier fly eggs in a constant temperature and humidity incubator at 30° C. and 70% relative humidity for incubation;

[0057] (3) raising the hatched black soldier fly larvae in wheat bran with a moisture content of 60% for 8 days;

[0058] (4) Using power 8W, wavelength 254nm, UV dose 46μW·s / cm 2 The larvae after being raised for 8 days in step (3) were irradiated with ultraviolet light for 15 minutes.

[0059] (5) inoculating 200 8-day-old black soldier fly larvae after ultraviolet irradiation into the substrate of step (1) for breeding, the substrate dry matter mass being 100 g, and placing in an environment with a temperature of 30° C. and a relative humidity of 70% for breeding for 12 days;

[0060] (6) After 12 days of feeding, the urban organic solid waste and black soldier flies were separated using an 80-mesh sieve, and all the insect sand and black soldier flies were weighed;

[0061] (7) The black soldier flies were stored at -80°C, and a portion of the substrate was weighed and stored at -80°C. The black soldier flies and the substrate were analyzed for insect body composition and substrate reduction, and the group was named T1.

[0062] Example 2

[0063] A method for promoting the growth of black soldier flies by ultraviolet induction comprises the following steps:

[0064] (1) Mix soybean meal, wheat bran and corn flour in a ratio of 1:1:1 as a substrate for feeding black soldier fly larvae;

[0065] (2) placing 20 g of black soldier fly eggs in a constant temperature and humidity incubator at 30° C. and 70% relative humidity for incubation;

[0066] (3) raising the hatched black soldier fly larvae in wheat bran with a moisture content of 60% for 8 days;

[0067] (4) inoculating 200 8-day-old black soldier fly larvae into the substrate of step (1), with a substrate dry matter mass of 100 g, and placing the substrate in an environment with a temperature of 30° C. and a relative humidity of 70% for 4 days;

[0068] (5) Using power 8W, wavelength 254nm, UV dose 46μW·s / cm 2 The larvae after being raised for 4 days in step (4) were irradiated with ultraviolet light for 15 minutes.

[0069] (6) After 8 days of continuous feeding, the urban organic solid waste and black soldier flies were separated using an 80-mesh sieve, and all the insect sand and black soldier flies were weighed;

[0070] (7) The black soldier flies were stored at -80°C, and part of the substrate was weighed and stored at -80°C. The black soldier flies and the substrate were analyzed for insect body composition and substrate reduction, and the group was named T2.

[0071] Example 3

[0072] A method for promoting the growth of black soldier flies by ultraviolet induction comprises the following steps:

[0073] (1) Mix soybean meal, wheat bran and corn flour in a ratio of 1:1:1 as a substrate for feeding black soldier fly larvae;

[0074] (2) placing 20 g of black soldier fly eggs in a constant temperature and humidity incubator at 30° C. and 70% relative humidity for incubation;

[0075] (3) raising the hatched black soldier fly larvae in wheat bran with a moisture content of 60% for 8 days;

[0076] (4) inoculating 200 8-day-old black soldier fly larvae into the substrate of step (1), with a substrate dry matter mass of 100 g, and raising them in an environment with a temperature of 30° C. and a relative humidity of 70% for 8 days;

[0077] (5) Using power 8W, wavelength 254nm, UV dose 46μW·s / cm 2 The larvae after being raised for 8 days in step (4) were irradiated with ultraviolet light for 15 minutes.

[0078] (6) After 4 days of continuous feeding, the urban organic solid waste and black soldier flies were separated using an 80-mesh sieve, and all the insect sand and black soldier flies were weighed;

[0079] (7) The black soldier flies were stored at -80°C, and part of the substrate was weighed and stored at -80°C. The black soldier flies and the substrate were analyzed for insect body composition and substrate reduction, and the group was named T3.

[0080] Example 4

[0081] A method for promoting the growth of black soldier flies by ultraviolet induction comprises the following steps:

[0082] (1) Mix soybean meal, wheat bran and corn flour in a ratio of 1:1:1 as a substrate for feeding black soldier fly larvae;

[0083] (2) placing 20 g of black soldier fly eggs in a constant temperature and humidity incubator at 30° C. and 70% relative humidity for incubation;

[0084] (3) raising the hatched black soldier fly larvae in wheat bran with a moisture content of 60% for 8 days;

[0085] (4) Using power 8W, wavelength 254nm, UV dose 46μW·s / cm 2 The larvae after being raised for 8 days in step (3) were irradiated with ultraviolet light for 15 minutes.

[0086] (5) inoculating 200 8-day-old black soldier fly larvae after ultraviolet irradiation into the substrate of step (1) for breeding, the substrate dry matter mass being 100 g, and placing in an environment with a temperature of 30° C. and a relative humidity of 70% for breeding for 4 days;

[0087] (6) Using power 8W, wavelength 254nm, UV dose 46μW·s / cm 2 The larvae raised for 4 days in step (5) were irradiated with an ultraviolet lamp for 15 minutes.

[0088] (7) After continuing to raise the fish for 4 days under the conditions of step (5), the fish were irradiated with an ultraviolet lamp for 15 minutes, with an ultraviolet power of 8 W, a wavelength of 254 nm, and an ultraviolet dose of 46 μW·s / cm 2 .

[0089] (8) After 4 days of continuous feeding, the urban organic solid waste and black soldier flies were separated using an 80-mesh sieve, and all the insect sand and black soldier flies were weighed;

[0090] (9) The black soldier flies were stored at -80°C, and a portion of the substrate was weighed and stored at -80°C. The black soldier flies and the substrate were analyzed for insect body composition and substrate reduction, and the group was named T4.

[0091] Example 5

[0092] A method for promoting the growth of black soldier flies by ultraviolet induction comprises the following steps:

[0093] (1) Mix soybean meal, wheat bran and corn flour in a ratio of 1:1:1 as a substrate for feeding black soldier fly larvae;

[0094] (2) placing 20 g of black soldier fly eggs in a constant temperature and humidity incubator at 30° C. and 70% relative humidity for incubation;

[0095] (3) raising the hatched black soldier fly larvae in wheat bran with a moisture content of 60% for 8 days;

[0096] (4) inoculating 200 8-day-old black soldier fly larvae into the substrate of step (1), with a substrate dry matter mass of 100 g, and raising them in an environment with a temperature of 30° C. and a relative humidity of 70% for 10 days;

[0097] (5) Using power 8W, wavelength 254nm, UV dose 46μW·s / cm 2 The larvae raised for 10 days in step (4) were irradiated with an ultraviolet lamp for 5 minutes.

[0098] (6) After the larvae of step (5) are inoculated into clean feed and continued to be raised for 2 days, the urban organic solid waste and black soldier flies are separated using an 80-mesh sieve, and all the insect sand and black soldier flies are weighed;

[0099] (7) The black soldier flies were stored at -80°C, and a portion of the substrate was weighed and stored at -80°C. The black soldier flies and the substrate were analyzed for insect body composition and substrate reduction, and the group was named T5.

[0100] Example 6

[0101] The difference between Example 6 and Example 5 is that the irradiation time in step (5) is different, and the irradiation time in step (5) is 10 minutes. The black soldier flies separated are named T6 group.

[0102] Example 7

[0103] The difference between Example 7 and Example 5 is that the irradiation time in step (5) is different, and the irradiation time in step (5) is 20 minutes. The black soldier flies separated are named T7 group.

[0104] Example 8

[0105] The difference between Example 8 and Example 5 is that the irradiation time in step (5) is different, and the irradiation time in step (5) is 30 minutes. The separated black soldier flies are named T8 group.

[0106] Comparative Example 1

[0107] (1) Mix soybean meal, wheat bran and corn flour in a ratio of 1:1:1 as a substrate for feeding black soldier fly larvae;

[0108] (2) placing 20 g of black soldier fly eggs in a constant temperature and humidity incubator at 30° C. and 70% relative humidity for incubation;

[0109] (3) raising the hatched black soldier fly larvae in wheat bran with a moisture content of 60% for 8 days;

[0110] (4) 200 8-day-old black soldier fly larvae were inoculated into a substrate of soybean meal: wheat bran: corn flour mixed at a ratio of 1:1:1, with a substrate dry matter mass of 100 g, and placed in an environment with a temperature of 30°C and a relative humidity of 70% for 12 days;

[0111] (5) After 12 days of feeding, the urban organic solid waste and black soldier flies were separated using an 80-mesh sieve, and all the insect sand and black soldier flies were weighed;

[0112] (6) The black soldier flies were stored at -80°C, and part of the substrate was weighed and stored at -80°C. The black soldier flies and the substrate were analyzed for insect body composition and substrate reduction, and the group was named CK.

[0113] The growth and development of the black soldier flies of Examples 1 to 5 and Comparative Example 1 were tested, and all data were tested three times and the average value was taken. The test results are shown in Table 1.

[0114] Table 1 Growth and development of black soldier flies in different groups

[0115]

[0116] From Table 1 we can see that:

[0117] (1) In Examples 1, 2 and 3, after the black soldier flies were exposed to ultraviolet light, the average body weight of the black soldier flies increased. Compared with the control group CK, the average body weight of the black soldier flies in the T1, T2 and T3 groups increased by 3.90%, 6.85% and 10.24%, respectively. The results showed that ultraviolet light exposure before inoculation, 4 days after inoculation and 8 days after inoculation promoted the weight of the black soldier flies, and ultraviolet light exposure 8 days after inoculation had the most obvious effect on the growth of the black soldier flies.

[0118] (2) Except for Example 4, there is no significant difference in the survival rate between Examples 1 to 3 and Comparative Example 1. It can be seen that appropriate ultraviolet irradiation has no obvious effect on the survival rate of black soldier flies, but frequent ultraviolet irradiation will affect the survival rate of black soldier flies. For example, in Example 4, ultraviolet irradiation was used before inoculation, 4 days after inoculation, and 8 days after inoculation. This practice greatly reduced the survival rate of black soldier flies.

[0119] (3) The feed conversion ratio and bioconversion rate can reflect the utilization of feed by larvae. The lower the feed conversion ratio and the higher the bioconversion rate, the better the bioconversion performance of black soldier flies for feed. Compared with comparative example 1 (CK), the feed conversion ratios in examples 2 (T2) and 3 (T3) were reduced by 3.38% and 6.36%, respectively, and the bioconversion rates were increased by 4.23% and 8.53%, respectively.

[0120] (4) The solid contents of Example 1 (T1), 2 (T2), and 3 (T3) were 0.75%, 0.74%, and 1.80% higher than that of Comparative Example 1 (CK), respectively, indicating that UV induction is beneficial to the accumulation of insect biomass of black soldier flies.

[0121] (5) Since the larvae in Examples 5 (T5), 6 (T6), 7 (T7), and 8 (T8) were irradiated after they entered the prepupal stage, the various indicators did not change significantly compared with those in Comparative Example 1 (CK).

[0122] The amino acids of the black soldier fly bodies of Examples 1 to 8 and Comparative Example 1 were measured, and the results are shown in Table 2:

[0123] Table 2 Amino acid content of black soldier flies in different groups

[0124] CK T1 T2 T3 T4 T5 T6 T7 T8 Threonine 1.54 1.93 1.74 1.84 1.91 1.57 1.56 1.64 2.00 Histidine 1.52 1.62 1.40 1.62 1.72 1.54 1.54 1.60 1.86 Valine 2.96 3.40 3.12 3.32 3.31 3.13 3.01 3.15 3.75 Methionine 0.58 0.81 0.73 0.81 0.85 0.65 0.61 0.67 0.75 Phenylalanine 2.09 2.26 2.05 2.22 2.27 2.17 2.09 2.24 2.66 Isoleucine 1.79 2.43 2.23 2.34 2.38 1.82 1.78 1.91 2.32 Leucine 2.95 3.22 2.96 3.16 3.21 3.00 2.97 3.13 3.52 Lysine 2.53 2.71 2.39 2.63 2.92 2.57 2.54 2.72 2.94 Essential amino acids 15.96 18.37 16.61 17.95 18.57 16.45 16.10 17.06 19.78 Aspartic acid 4.11 4.83 4.58 4.66 4.88 4.27 4.13 4.41 5.53 Glutamate 4.67 5.68 5.43 5.24 5.61 4.87 4.71 4.90 5.25 Serine 1.60 1.79 1.64 1.68 1.73 1.63 1.61 1.58 2.13 Proline 1.92 3.33 2.22 2.35 2.39 1.87 1.85 1.88 2.12 Glycine 2.53 2.58 2.19 2.42 2.39 2.55 2.54 2.58 2.97 Arginine 1.89 2.24 2.13 2.37 2.42 1.83 1.89 1.99 2.27 Alanine 2.78 3.19 3.10 3.17 3.23 2.82 2.79 2.76 3.19 Tyrosine 2.89 3.06 2.95 3.35 3.31 2.91 2.97 3.00 3.52 Cysteine 0.04 0.01 0.01 0.06 0.08 0.05 0.04 0.07 0.06 Sum of non-essential amino acids 22.44 26.71 24.24 25.31 26.03 22.79 22.54 23.16 27.04

[0125] From Table 2 we can see that:

[0126] (1) After the black soldier fly was exposed to ultraviolet light, the contents of essential and non-essential amino acids in the insect body increased to varying degrees, indicating that ultraviolet induction at any stage can promote the synthesis of histidine in the insect body.

[0127] (2) After the black soldier fly bodies were subjected to ultraviolet irradiation, the essential amino acid and non-essential amino acid contents of the bodies in all examples were increased, indicating that ultraviolet induction treatment was beneficial for the black soldier fly to synthesize more amino acids, and the increase in the essential amino acid content enabled the bodies to obtain higher utilization value.

[0128] By measuring the conventional nutritional indicators such as protein, fat and ash in the dry weight of black soldier flies prepupae in each group, the changes in the nutritional composition of black soldier flies in different groups were determined. The results are as follows: Figure 2 As shown. Figure 2 It can be seen that:

[0129] The protein content of black soldier flies in Examples 1 (T1), 2 (T2), 3 (T3), 4 (T4), and 8 (T8) was higher than that in the comparative example 1 (CK), and the protein content of black soldier flies in Examples 5 (T5), 6 (T6), and 7 (T7) was lower than that in the comparative example 1 (CK). The results show that ultraviolet induction before and during larval rearing is beneficial to the synthesis of black soldier fly protein, and that a longer period of ultraviolet induction is required to promote protein synthesis after the larvae enter the prepupae stage, but the final synthesized protein content is higher.

[0130] After UV induction, the fat content of the insects in all examples decreased compared with the control example 1 (CK). The results showed that UV induction inhibited the synthesis of insect body fat.

[0131] By measuring the remaining substrate, the changes in the substrate reduction rate of different groups were determined. The substrate here refers to the feed with a ratio of 1:1:1 of soybean meal, wheat bran and corn flour used in the larval feeding stage. The results are as follows: Figure 3 shown.

[0132] from Figure 3 It can be seen that:

[0133] The substrate dry matter reduction rate in Examples 1 (T1), 2 (T2), and 3 (T3) was higher than that in Comparative Example 1 (CK), and the substrate dry matter reduction rate in Example 4 (T4) was lower than that in Comparative Example 1 (CK). The results show that UV induction before inoculation, 4 days after inoculation, and 8 days after inoculation is conducive to the black soldier flies to utilize more substrates, and UV induction in all three stages will have an adverse effect on the black soldier flies to utilize the substrate. In Examples 5 (T5), 6 (T6), 7 (T7), and 8 (T8), since UV induction was performed after the larvae entered the prepupae stage after 10 days of feeding, the substrate dry matter reduction rate did not change significantly compared with the Comparative Example 1 (CK).

[0134] In summary, the present invention respectively measured the weight difference, survival rate, feed conversion ratio, bioconversion rate, black soldier fly nutritional index, and substrate reduction rate of black soldier flies. The analysis results showed that appropriate ultraviolet induction of black soldier flies can promote the growth of black soldier flies.

[0135] The embodiments provided above are not intended to limit the scope of the present invention, and the steps described are not intended to limit the execution order thereof. Those skilled in the art may make obvious improvements to the present invention in combination with existing common knowledge, which also fall within the scope of protection defined by the claims of the present invention.

Claims

1. A method for promoting the growth of black soldier flies and promoting protein synthesis in the insect body by ultraviolet induction, characterized in that: The method utilizes ultraviolet induction to stimulate black soldier flies to improve the feeding behavior and metabolic function of larvae to promote the biomass accumulation of black soldier flies, and comprises the following steps: (1) placing black soldier fly eggs in a constant temperature and humidity incubator for hatching to obtain black soldier fly larvae; (2) raising the hatched black soldier fly larvae in wheat bran with a moisture content of 60% for 8 days; (3) inoculating 8-day-old black soldier fly larvae into feed for feeding; (4) Before larval inoculation and / or during feeding, a UV light with a power of 8 W, a wavelength of 254 nm, and a UV dose of 46 μW·s / cm 2 The ultraviolet light is used for irradiation treatment; (5) After 12 days of rearing, the substrate and the black soldier flies were separated and the black soldier flies were stored at -80°C.

2. The method according to claim 1, characterized in that The incubation environment in step (1) is a constant temperature and humidity incubator with an ambient temperature of 25-30° C. and a relative humidity of 60-70%.

3. The method according to claim 1, characterized in that The moisture content of the feed in step (3) is 70%, the composition ratio is soybean meal: wheat bran: corn flour 1:1:1, and the dry matter weight is 100g.

4. The method according to claim 1, characterized in that: In step (3), the inoculation ratio of the black soldier fly larvae is 3 to 4 black soldier fly larvae per 1 to 2 g of dry matter feed, and the feeding is carried out in an environment with a temperature of 30° C. and a relative humidity of 70%.

5. The method according to claim 1, characterized in that In step (4), ultraviolet light irradiation is performed before larvae inoculation and / or during larvae rearing.

6. The method according to claim 1, characterized in that In step (4), the ultraviolet light irradiation is carried out after the larvae have been reared for 4 or 8 days.

7. The method according to claim 1, characterized in that In step (4), the ultraviolet light irradiation is carried out after the larvae have been reared for 10 days and have entered the prepupal stage. After the ultraviolet light irradiation, the larvae are inoculated into clean feed and reared for another 2 days.

8. The method according to claim 1, characterized in that The ultraviolet light irradiation time in step (4) is 5 to 30 minutes.

9. The method according to claim 1, characterized in that: In step (5), an 80-mesh sieve is used to separate the black soldier flies and the substrate.

10. Use of the black soldier fly obtained according to the method according to any one of claims 1 to 9 in treating organic waste.

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