An anti-aging microemulsion composition, microemulsion, preparation method and application

Abstract

The present application discloses an anti-aging microemulsion composition, microemulsion, preparation method and application. The anti-aging microemulsion composition of the present application, in parts by mass, includes the following components: 8 to 10 parts of oil, 1 to 5 parts of emulsifier, 0.1 to 2 parts of oil phase actives, 0.01 to 5 parts of water phase actives, 1 to 5 parts of co-emulsifiers and 15 to 25 parts of the first solvent; the oil phase actives include dipalmitoyl hydroxyproline; the oil includes tri(ethylhexanoic acid) glyceryl ester and isononanoic acid isononyl ester in a mass ratio of (1 to 7): (1 to 7); the emulsifier is PolyAquol 2W; the water phase actives include β-glucan and sturgeon roe sauce extract in a mass ratio of (0.01 to 0.1): (2 to 5). The anti-aging microemulsion composition of the present application can regulate the functions of adipocytes and extracellular matrix to achieve firming and anti-aging effects.

Description

Technical Field

[0001] The present application belongs to the technical field of cosmetics, and in particular relates to an anti-aging microemulsion composition, a microemulsion, a preparation method and an application. Background Art

[0002] Affected by endogenous aging of the body and external factors such as ultraviolet rays and environmental pollution, the skin will develop wrinkles, looseness, sagging and other aging characteristics over time. During the aging process, the number, size and composition of fat cells will change. The subcutaneous fat layer (subcutaneous fat) infiltrates into the dermis with age. This fat penetration damages the dermis, reduces the elasticity of the skin and induces sagging. In addition to the damage to the structural integrity of the dermis caused by fat infiltration, the infiltrated fat may also have a negative impact on the condition of the dermis through physiological effects within the dermis. Aging causes the subcutaneous fat cells in the female face to become smaller, resulting in facial atrophy. Facial fat cells affect the mechanical properties of the skin and reduce the critical mechanical strain for wrinkle formation. Fat infiltration into the dermis also promotes the formation of transient wrinkles caused by facial expressions, which in turn leads to an increase in static wrinkles. Therefore, regulatory schemes based on adipocytes and the extracellular matrix of the dermis are key goals for anti-aging treatment.

[0003] There are many anti-aging products on the market, which mainly promote the skin's self-repair ability by adding various antioxidants, vitamins, plant extracts, and ingredients that promote collagen synthesis, such as peptides and amino acids, to achieve anti-aging effects. These products can only solve problems in the skin layer and fascia layer, and cannot effectively regulate fat cells and extracellular matrix. If you want to get a full face, you must inject it. Fat filling is not safe, and it is easy to "fill" too much and cause "steaming" phenomenon.

[0004] Although the existing anti-aging lotions can achieve the purpose of anti-aging to a certain extent, there are still some problems. First, the particle size of the existing anti-aging lotions is relatively large, generally at the micron level, which limits its absorbency and bioavailability. Secondly, the existing anti-aging lotions mainly achieve the purpose of anti-aging by adding various ingredients. This method of addition may have safety issues, such as adding too many chemicals may irritate the skin. In addition, the effects of the existing anti-aging lotions in regulating the synthesis of fat cells and the synthesis of extracellular matrix are also relatively limited, which limits the depth and durability of its anti-aging. Therefore, how to prepare an anti-aging lotion with smaller particle size, better absorbency, higher safety and better anti-aging effect is a problem that needs to be solved urgently in this field. Summary of the invention

[0005] Purpose of the invention: In view of the problems and deficiencies in the prior art, the present application provides an anti-aging microemulsion composition, microemulsion, preparation method and application. The anti-aging microemulsion composition of the present application embodiment can regulate the functions of fat cells and extracellular matrix to achieve firming and anti-aging effects.

[0006] Technical solution: The embodiment of the present application provides an anti-aging microemulsion composition, which includes the following components, in parts by mass: 8 to 10 parts of oil, 1 to 5 parts of emulsifier, 0.1 to 2 parts of oil phase active matter, 0.01 to 5 parts of water phase active matter, 1 to 5 parts of co-emulsifier and 15 to 25 parts of a first solvent; the oil phase active matter includes dipalmitoyl hydroxyproline; the oil includes tri(ethylhexanoic acid)glyceryl and isononyl isononanoate in a mass ratio of (1 to 7): (1 to 7); the emulsifier is PolyAquol 2W; the water phase active matter includes β-glucan and sturgeon roe extract in a mass ratio of (0.01 to 0.1): (2 to 5).

[0007] In some embodiments, the co-emulsifier includes pentylene glycol.

[0008] The present invention provides a method for preparing an anti-aging microemulsion composition, comprising the following steps:

[0009] The oil phase active substance is mixed with the oil, stirred to dissolve, and an emulsifier is added. After stirring and dissolving at 80°C to 85°C, an auxiliary emulsifier is added to obtain an oil phase;

[0010] The first solvent is heated to 80° C. to 85° C. as a hot water phase, wherein, based on the total mass of the first solvent, 2% to 5% of the hot water by mass of the first solvent is taken as the first hot water phase, and the remaining hot water phase is taken as the second hot water phase;

[0011] The first hot water phase is added to the oil phase at a first rate to form an oil-in-water emulsion, and in the process of dripping the first hot water phase, stirring is continued at a first stirring rate; wherein the first rate is v 1 , v 1 The value of is 50s / drop to 100s / drop, and the first stirring rate is R 1 , R 1 The value is 150rpm / min~250rpm / min;

[0012] The aqueous phase active substances including β-glucan and sturgeon roe extract are dissolved in the second hot water phase, and after being dissolved, they are added to the water-in-oil emulsion system at a second rate, and stirred at a second stirring rate; wherein the second rate is v 2 , v 2 The value of is 5s / drop to 15s / drop; the second stirring rate is R 2 , R 2The value is 150rpm / min~250rpm / min;

[0013] The mixture is stirred evenly to obtain an anti-aging microemulsion composition.

[0014] The embodiment of the present application also provides an anti-aging microemulsion, comprising the above-mentioned anti-aging microemulsion composition, wherein the mass percentage of the anti-aging microemulsion composition in the anti-aging microemulsion is 20% to 40% based on the total mass of the anti-aging microemulsion.

[0015] In some embodiments, the anti-aging microemulsion further includes a gel phase, which includes, by weight, 0.01 to 0.5 parts of xanthan gum, 0.1 to 1 parts of ammonium acryloyldimethyltaurate / VP copolymer, 1 to 5 parts of AQUAXYL and 40 to 60 parts of water.

[0016] In some embodiments, the gel phase further comprises 1 to 3 parts of niacinamide and 0.5 to 1.5 parts of asiaticaside, based on weight.

[0017] In some embodiments, the gel phase further comprises 1 to 3 parts by weight of a second solvent, wherein the second solvent comprises glycerol.

[0018] The present application also provides a method for preparing an anti-aging microemulsion, comprising the following steps:

[0019] preparing an anti-aging microemulsion composition;

[0020] preparing a gel phase;

[0021] The gel phase is added to the anti-aging microemulsion composition at a third rate, and the stirring is continued at a third stirring rate, wherein the third rate is v 3 , v 3 The value is 5s / drop to 15s / drop, and the third stirring rate is R 3 , R 3 The value is 600rpm / min~800rpm / min.

[0022] The embodiments of the present application also provide an application of the anti-aging microemulsion composition, or the anti-aging microemulsion composition prepared by the preparation method, or the anti-aging microemulsion, or the anti-aging microemulsion prepared by the preparation method in the preparation of anti-aging products.

[0023] Beneficial effects: The present application provides an anti-aging microemulsion composition, microemulsion, preparation method and application. The anti-aging microemulsion composition of the present application comprises the following components, in parts by mass: 8 to 10 parts of oil, 1 to 5 parts of emulsifier, 0.1 to 2 parts of oil phase actives, 0.01 to 5 parts of water phase actives, 1 to 5 parts of co-emulsifiers and 15 to 25 parts of the first solvent; the oil phase actives include dipalmitoyl hydroxyproline; the oil includes tri(ethylhexanoic acid)glyceryl and isononanoyl isononanoate in a mass ratio of (1 to 7): (1 to 7); the emulsifier is PolyAquol 2W; the water phase actives include β-glucan and sturgeon roe extract in a mass ratio of (0.01 to 0.1): (2 to 5). The anti-aging microemulsion composition of the present application can regulate the functions of adipocytes and extracellular matrix to achieve firming and anti-aging effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0025] Figure 1 The effect of the proportion of the first hot water phase in the anti-aging microemulsion composition prepared in the examples of the present application on the performance of the microemulsion;

[0026] Figure 2 The effect of the addition rate of the first hot water phase on the performance of the microemulsion in the anti-aging microemulsion composition prepared in the examples of the present application;

[0027] Figure 3 The effect of the rate at which the second hot water phase prepared in the examples of the present application is added to the water-in-oil emulsion on the performance of the microemulsion;

[0028] Figure 4 The stirring rate during the addition of the second hot water phase in the embodiment of the present application;

[0029] Figure 5 The following are typical examples of skin ultrasound using the microemulsion of the present application, wherein the left picture is the image before use, and the right picture is the image after use;

[0030] Figure 6 This is an image of a typical case of skin nasolabial folds without using the microemulsion prepared in the examples of the present application;

[0031] Figure 7 This is an image of a typical case of improving skin nasolabial folds using the microemulsion prepared in an embodiment of the present application. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present application. In addition, in the description of the present application, the term "including" means "including but not limited to". The terms first, second, third, etc. are used only as markings, and no numerical requirements or order are imposed. Various embodiments of the present application may exist in the form of a range; it should be understood that the description in the form of a range is only for convenience and simplicity, and should not be understood as a hard limitation on the scope of the present application; therefore, it should be considered that the range description has specifically disclosed all possible sub-ranges and single values ​​within the range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the numbered range, such as 1, 2, 3, 4, 5 and 6, which are applicable regardless of the range. Additionally, whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range.

[0033] Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art in the field described in this application. Although only preferred methods and materials are described in this application, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of this application. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail. Unless otherwise specified, "%" is a percentage based on mass.

[0034] The microemulsions of the prior art have the following problems: (1) Particle size problem: The particle size of the existing anti-aging lotions is relatively large, generally at the micron level, which limits their absorbability and bioavailability. (2) Safety problem: The existing anti-aging lotions mainly achieve the purpose of anti-aging by adding various ingredients. This method of addition may have safety problems. For example, adding too many chemicals may irritate the skin. (3) Anti-aging effect problem: The existing anti-aging lotions are also relatively limited in their effect on regulating the synthesis of fat cells and the synthesis of extracellular matrix, which limits their depth and durability of anti-aging.

[0035] In order to solve the problems of the prior art, the embodiment of the present application provides an anti-aging microemulsion composition, which includes the following components, measured by mass: 8 to 10 parts of oil, 1 to 5 parts of emulsifier, 0.1 to 2 parts of oil phase active matter, 0.01 to 5 parts of water phase active matter, 1 to 5 parts of co-emulsifier and 15 to 25 parts of a first solvent; the oil phase active matter includes dipalmitoyl hydroxyproline; the oil includes tri(ethylhexanoin)glyceryl and isononyl isononanoate in a mass ratio of (1 to 7): (1 to 7); the emulsifier is PolyAquol 2W; the water phase active matter includes β-glucan and sturgeon roe extract in a mass ratio of (0.01 to 0.1): (2 to 5). The anti-aging microemulsion composition of the embodiment of the present application can regulate the synthesis of fat cells and the synthesis of extracellular matrix, including regulating the synthesis of fat cells by adding sturgeon roe extract, and promoting the synthesis of collagen, glycosaminoglycans and elastin in the extracellular matrix by compounding with dipalmitoyl hydroxyproline; in addition, the addition of β-glucan in the implementation of the present application can enhance the resistance of new fat cells and extracellular matrix, thereby enhancing the anti-aging effect.

[0036] In some embodiments, the anti-aging microemulsion composition of the present application is prepared into a microemulsion, and dipalmitoyl hydroxyproline is encapsulated in the microemulsion, which improves the utilization of its bioactive substances, thereby prolonging its residence time in the skin and improving the anti-aging effect.

[0037] In some embodiments, the oil comprises triethylhexanoin and isononyl isononanoate in a mass ratio of 3:5.

[0038] In some embodiments, the first solvent includes water.

[0039] In some embodiments, the anti-aging microemulsion composition includes, by mass: 3 parts of triethylhexanoin, 5 parts of isononyl isononanoate, 3 parts of emulsifier PolyAquol 2W, 1 part of oil phase active substance dipalmitoyl hydroxyproline, 0.05 parts of water phase active substance β-glucan and 2 parts of water phase active substance sturgeon roe extract, 3 parts of co-emulsifier pentylene glycol and 20 parts of water.

[0040] In some embodiments, the anti-aging microemulsion composition is prepared by the following method:

[0041] The oil phase active substance is mixed with the oil, stirred to dissolve, and an emulsifier is added. After stirring and dissolving at 80°C to 85°C, an auxiliary emulsifier is added to obtain an oil phase;

[0042] The first solvent is heated to 80° C. to 85° C. as a hot water phase, wherein, based on the total mass of the first solvent, 2% to 5% of the hot water by mass of the first solvent is taken as the first hot water phase, and the remaining hot water phase is taken as the second hot water phase;

[0043] The first hot water phase is added to the oil phase at a first rate to form an oil-in-water emulsion. During the process of dripping the first hot water phase, stirring is continued at a first stirring rate; wherein the first rate is v 1 , v 1 The value of is 50s / drop to 100s / drop, and the first stirring rate is R 1 , R 1 The value is 150rpm / min~250rpm / min;

[0044] The aqueous phase active substances including β-glucan and sturgeon roe extract are dissolved in the second hot water phase, and the aqueous phase mixture obtained after the dissolution is added to the oil-in-water emulsion system at a second rate, and the stirring is continued at a second stirring rate; wherein the second rate is v 2 , v 2 The value of is 5s / drop to 15s / drop; the second stirring rate is R 2 , R 2 The value is 150rpm / min~250rpm / min;

[0045] The mixture is stirred evenly to obtain an anti-aging microemulsion composition.

[0046] In some embodiments, the first rate v 1 The value of can be any value among 50s / drop, 60s / drop, 70s / drop, 80s / drop, 90s / drop, 100s / drop, or a range consisting of any two values.

[0047] In some embodiments, the first stirring rate R 1 The value can be any value among 150rpm / min, 200rpm / min, 250rpm / min, or a range consisting of any two values.

[0048] In some embodiments, the second rate v 2 The value can be any value of 5s / drop, 10s / drop, 15s / drop, or a range consisting of any two values.

[0049] In some embodiments, the second stirring rate R 2 The value can be any value among 150rpm / min, 200rpm / min, 250rpm / min, or a range consisting of any two values.

[0050] In some embodiments, based on the total mass of the first solvent, the mass percentage of the first hot water phase to the first solvent is any value of 2%, 3%, 4%, 5%, or a range consisting of any two values.

[0051] In some embodiments, based on the total mass of the first solvent, the mass percentage of the second hot water phase to the first solvent is 95% to 98%, such as the mass percentage of the second hot water phase to the first solvent is 95%, 96%, 97%, 98%, or any range consisting of any two values.

[0052] In some specific embodiments, the anti-aging microemulsion composition of the present application is prepared by the following method:

[0053] 3 parts by mass of glyceryl tri(ethylhexanoate), 5 parts by mass of isononyl isononanoate, and 1 part by mass of dipalmitoyl hydroxyproline were mixed and stirred to dissolve, 3 parts by mass of emulsifier POLYAQUOL 2W were added, and after stirring and dissolving at 80°C to 85°C, 3 parts by mass of co-emulsifier pentylene glycol were added to obtain an oil phase;

[0054] 20 parts by weight of the first solvent are heated to 80° C. to 85° C. as a hot water phase, wherein, based on the total weight of the first solvent, 2% of the hot water by weight of the first solvent is taken as the first hot water phase, and the remaining hot water phase is taken as the second hot water phase;

[0055] The first hot water phase is added to the oil phase at a first rate of 50 s / drop to form a water-in-oil emulsion. During the process of dropping the first hot water phase, stirring is continued at a first stirring rate of 200 rpm / min;

[0056] The aqueous phase active substance including 0.05 parts by mass of β-glucan and 2 parts by mass of sturgeon roe extract is dissolved in a second hot water phase accounting for 98% by mass of the first solvent, and the aqueous phase mixture obtained after the dissolution is added to the water-in-oil emulsion system at a second rate of 10s / drop, and the stirring is continued at a second stirring rate of 200rpm / min;

[0057] The mixture is stirred evenly to obtain an anti-aging microemulsion composition.

[0058] The present application also provides an anti-aging microemulsion, which includes the above-mentioned anti-aging microemulsion composition. Based on the total mass of the anti-aging microemulsion, the mass percentage of the anti-aging microemulsion composition in the anti-aging microemulsion is 20% to 40%, such as the mass percentage of the anti-aging microemulsion composition in the anti-aging microemulsion is any value of 20%, 25%, 30%, 35%, 40%, or a range consisting of any two values.

[0059] In some embodiments, the anti-aging microemulsion of the present application also includes a gel phase, which includes, by weight, 0.01 to 0.5 parts of xanthan gum, 0.1 to 1 parts of ammonium acryloyldimethyltaurate / VP copolymer, 1 to 5 parts of AQUAXYL and 40 to 60 parts of water.

[0060] For example, in some embodiments, the mass percentage of the anti-aging microemulsion composition in the anti-aging microemulsion is any value of 20%, 25%, 30%, 35%, 40%, or a range consisting of any two values.

[0061] In some embodiments, the anti-aging microemulsion further includes a gel phase, which includes, by weight, 0.01 to 0.5 parts of xanthan gum, 0.1 to 1 parts of ammonium acryloyldimethyltaurate / VP copolymer, 1 to 5 parts of AQUAXYL and 40 to 60 parts of water.

[0062] In some embodiments, the gel phase further comprises 1 to 3 parts of niacinamide and 0.5 to 1.5 parts of asiaticaside, based on weight.

[0063] In some specific embodiments, the gel phase includes, by mass, 0.1 parts of xanthan gum, 0.3 parts of ammonium acryloyldimethyltaurate / VP copolymer, 3 parts of AQUAXYL, 2 parts of niacinamide, 1 part of asiaticaside and 3 parts of glycerin, and water is added to 100 parts by mass.

[0064] The anti-aging microemulsion of the present application adopts a variety of bioactive substance compounding technologies, including: in order to enhance the overall effect of the anti-aging lotion, the compound use of Centella asiatica that can promote skin health, niacinamide that promotes skin autophagy, and AQUAXYL (xylitol) that promotes skin barrier health and moisture, so that the ingredients have a synergistic effect, thereby further improving the anti-aging effect of the product.

[0065] In some embodiments, the gel phase further includes 1 to 3 parts by weight of a second solvent, and the second solvent includes glycerol.

[0066] In some specific embodiments, the anti-aging microemulsion further includes a preservative. Based on the total mass of the microemulsion, the anti-aging microemulsion includes 0.1% to 1% by mass of the preservative.

[0067] In some specific embodiments, the gel phase includes, by mass, 0.1 parts of xanthan gum, 0.3 parts of ammonium acryloyldimethyltaurate / VP copolymer, 3 parts of AQUAXYL, 2 parts of niacinamide, 1 part of asiaticaside, 3 parts of glycerin, 0.4 parts of preservatives, and 53.15 parts of water.

[0068] The present application avoids adding too many chemicals: by adding the above-mentioned bioactive substances, adding too many chemicals is avoided, thereby improving the safety of the product, and the anti-aging microemulsion of the present application enhances the deep anti-aging effect: by the above-mentioned method of regulating the synthesis of fat cells and the synthesis of extracellular matrix, the anti-aging effect can be enhanced, and the anti-aging depth and durability of the product can be improved.

[0069] In some specific embodiments, the anti-aging microemulsion of the present application is composed of the following components by weight:

[0070] Anti-aging microemulsion composition: 3 parts of triethylhexanoin, 5 parts of isononyl isononanoate, 3 parts of emulsifier PolyAquol2W, 1 part of oil phase active substance dipalmitoyl hydroxyproline, 0.05 parts of water phase active substance β-glucan and 2 parts of water phase active substance sturgeon roe extract, 3 parts of co-emulsifier pentylene glycol and 20 parts of water;

[0071] Gel phase: 0.1 parts xanthan gum, 0.3 parts ammonium acryloyldimethyltaurate / VP copolymer, 3 parts AQUAXYL, 2 parts niacinamide, 1 part asiaticaside, 3 parts glycerin and 0.4 parts preservatives;

[0072] Water: Water was added to 100 parts by mass.

[0073] The present application also provides a method for preparing an anti-aging microemulsion, comprising the following steps:

[0074] preparing an anti-aging microemulsion composition;

[0075] preparing a gel phase;

[0076] The gel phase is added to the anti-aging microemulsion composition at a third rate, and the stirring is continued at a third stirring rate, wherein the third rate is v 3 , v 3 The value is 5s / drop to 15s / drop, and the third stirring rate is R 3 , R 3 The value is 600rpm / min~800rpm / min.

[0077] In some embodiments, the third rate v 3 The value can be any value among 5s / drop, 10s / drop, 15s / drop, or a range consisting of any two values.

[0078] In some embodiments, the third stirring rate of the present application is R 3 The value can be any value among 600rpm / min, 700rpm / min, 800rpm / min, or a range consisting of any two values.

[0079] The volume range of "drop" in this application is 0.03mL to 0.07mL. In the specific embodiment of this application, the volume of each drop is controlled at 0.05mL.

[0080] In some embodiments, the prepared gel phase is added to the anti-aging microemulsion composition system at a speed of 5 s / drop, and the stirring speed is 600 rpm / min. The anti-aging microemulsion is obtained by stirring evenly.

[0081] The present application does not require homogenization or high-pressure homogenization equipment. The particle size of the anti-aging lotion can be controlled below 500 nm by simply adjusting the stirring speed and the formula composition and proportion, which can greatly improve its absorbability and bioavailability.

[0082] The anti-aging microemulsion in the embodiments of the present application has the following effects: (1) The microemulsion prepared in the embodiments of the present application has a smaller particle size and better absorbability: The particle size of the anti-aging emulsion of the present application is less than 500nm, which is smaller than the existing micron-level anti-aging emulsion. Due to the nanometer-level particle size, the anti-aging emulsion of the present application has a larger specific surface area, so that it can be better absorbed by the skin, thereby improving its bioavailability; (2) Higher safety: The anti-aging emulsion of the present application achieves the purpose of anti-aging by regulating the synthesis of fat cells and the synthesis of extracellular matrix, and has stronger targeting and specificity. The present application achieves anti-aging by regulating physiological functions, which not only has better effects, but also has higher safety, and avoids the possible irritation to the skin caused by adding too many chemicals and too many functions; (3) Better anti-aging effect and stronger durability: The anti-aging emulsion of the present application has a better effect in regulating the synthesis of fat cells and the synthesis of extracellular matrix, so it can more deeply combat skin aging. And because the anti-aging lotion of the present application has better absorbability and bioavailability, its anti-aging effect can be more lasting; (4) The preparation process is simple and can be mass-produced: The preparation process of the anti-aging lotion of the present application is simple, does not require homogenization and high-pressure homogenization equipment, only requires stirring, is suitable for large-scale production, greatly reduces production costs, and improves market competitiveness.

[0083] The anti-aging microemulsion in the embodiment of the present application can be used to prepare anti-aging products. The anti-aging products prepared in the present application can be widely used in the field of cosmetics. For example, the anti-aging emulsion of the present application can be used not only for anti-aging of facial skin, but also for skin in other parts, such as neck, hands, etc. In addition, the anti-aging emulsion of the present application can also be used for different types of skin, such as dry skin, oily skin, mixed skin, etc.

[0084] Source of raw materials: Triethylhexanoin and isononyl isononanoate are from Nisshin OilliO Group, Ltd; POLYAQUOL 2W is from Innovacos Biotech (Shanghai) Co., Ltd.; dipalmitoyl hydroxyproline is from LABORATOIRES SETHIC INNOVATIONS; β-glucan is from Sichuan Selicon Biotech Co., Ltd.; sturgeon roe extract is from Dongguan Vichy Technology Co., Ltd.; niacinamide is from DSM Nutritional Products Ltd.; asiatica glycoside is from Shanghai Jiakai Biotech Co., Ltd.; AQUAXYL (xylitol) is from SEPPIC SA.

[0085] Example 1: Preparation of anti-aging microemulsion

[0086] Step (1): Preparation of anti-aging microemulsion composition

[0087] 1) Dipalmitoylhydroxyproline is mixed with oil component 1 and oil component 2, stirred to dissolve, an emulsifier is added, and after stirring to dissolve at 80° C. to 85° C., pentanediol is added to obtain an oil phase;

[0088] 1) Heat purified water to 80°C-85°C to form a hot water phase for later use;

[0089] 2) Take 0.4 parts by mass of hot water as the first hot water phase (2% of the total amount of hot water) and add it to the oil phase at a rate of 50 seconds per drop to form a water-in-oil emulsion. Stir continuously during the process of adding the hot water of phase B at a stirring speed of 200 rpm / min;

[0090] 3) dissolving the aqueous phase active substances in phase C including β-glucan and sturgeon roe extract in 19.6 parts by mass of hot water (second hot water phase), and after dissolution, adding them into the water-in-oil emulsion system at a rate of 10 s / drop, and stirring at a speed of 200 rpm / min;

[0091] 4) stirring evenly to obtain an anti-aging microemulsion composition.

[0092] Table 1 Anti-aging microemulsion composition components of microemulsion

[0093]

[0094] Step (2): Preparation of gel phase

[0095] In the above technical solution, the preparation method of the microemulsion gel phase is:

[0096] 1) Mix xanthan gum, ammonium acryloyldimethyltaurate / VP copolymer and glycerol, add pure water and stir to swell to a uniform solution;

[0097] 2) Add nicotinamide, asiatica glycoside, AQUAXYL and preservatives to the above system to obtain a gel phase.

[0098] Table 2 Gel phase components of microemulsion

[0099]

[0100] Step (3): Preparation of microemulsion

[0101] The prepared gel phase was added into the anti-aging microemulsion composition system at a speed of 5 s / drop, and the stirring speed was 600 rpm / min. The anti-aging microemulsion was obtained by stirring evenly.

[0102] The particle size of the microemulsion was measured using a Zetasizer Nano series particle size analyzer at 25°C. Deionized water was used as a dispersant and the microemulsion was diluted 1000 times for measurement. The results showed that the average particle size of the microemulsion was 410.7nm and the polydispersity index PDI was 0.165. Both the dispersion and particle size met the expected requirements.

[0103] Example 2: Screening of oil phase

[0104] Since dipalmitoyl hydroxyproline (DPHP) is an oil-soluble active ingredient, it needs to be pre-dissolved in oils and fats for use. Due to the influence of polarity, its solubility in different oils and fats is different, and the encapsulation amount is positively correlated with product performance. Therefore, screening a suitable oil composition is crucial for the encapsulation of dipalmitoyl hydroxyproline. According to the principle of like dissolves like, due to the electrical interaction between polar molecules, solutes composed of polar molecules are easily soluble in solvents composed of polar molecules, and are difficult to dissolve in solvents composed of non-polar molecules; solutes composed of non-polar molecules are easily soluble in solvents composed of non-polar molecules, and are difficult to dissolve in solvents composed of polar molecules. In order to explore the best oils and fats and proportions, the inventor team of the applicant screened non-polar oil squalane, medium-polar oil tri(ethylhexanoic acid) glyceryl ester, and high-polar oil isononanoic acid isononyl ester for encapsulation treatment of dipalmitoyl hydroxyproline, and judged and screened the appropriate matching form and matching ratio by analyzing the state and stability of the microemulsion composition. The composition and test results of the oil phase are shown in Table 3. From the results in Table 3, it can be seen that the dipalmitoyl hydroxyproline (Proline) in the components of the embodiments of the present application is a derivative of hydroxyproline, and hydroxyproline is a polar amino acid containing a hydroxyl group (-OH) in its molecular structure. Therefore, dipalmitoyl hydroxyproline as its derivative has a certain polarity, and its polarity is between tri(ethylhexanoin)glyceryl and isononyl isononanoate. The inventor team of the applicant obtained the optimal oil phase combination including dipalmitoyl hydroxyproline by screening suitable oil types and proportions.

[0105] Table 3 Effect of different oil phase compositions on emulsion properties

[0106]

[0107] After screening and comparison, an implementation scheme with better encapsulation efficiency was obtained in the examples of the present application, in which tri(ethylhexanoic acid)glyceryl and isononyl isononanoate in a mass ratio of 3:5 were selected as the oil phase to encapsulate dipalmitoyl hydroxyproline.

[0108] Example 3: Screening of emulsifiers

[0109] Different emulsifiers have different molecular characteristics. They stabilize emulsions by reducing interfacial tension between phases, increasing steric hindrance and electrostatic repulsion between droplets. The mass fraction of the emulsifier directly affects the stability of the emulsion. In addition, the amount of emulsifier added also affects the particle size and clarity index of the emulsion. However, excessive emulsifier addition can cause skin irritation. Therefore, choosing the right emulsifier and controlling the appropriate amount are the key to improving the stability and safety of the emulsion.

[0110] Different emulsifiers have different emulsifying abilities for oils and fats. PolyAquol 2W is a nonionic emulsifier with self-emulsifying properties, which can form a stable emulsion without the need for co-emulsifiers or other auxiliary factors. POLYAQUOL LW is an ideal raw material for preparing small particle size emulsions, so the effects of these two emulsifiers were tested in terms of stability and appearance.

[0111] Compared with the components in Table 1, the composition and proportion of the emulsifier are partially adjusted in the examples of the present application, as shown in Table 3, and the remaining components remain consistent with Table 1.

[0112] Table 4 Effect of emulsifier type and dosage on emulsion properties

[0113]

[0114] By selecting the type and amount of the emulsifier, 3 parts by mass of POLYAQUOL 2W emulsifier was added to the system to obtain a microemulsion with good encapsulation efficiency.

[0115] Example 4: Effect of preparation process on microemulsion properties

[0116] In the anti-aging microemulsion composition in the embodiment of the present application, the added water includes a first hot water phase and a second hot water phase. The first hot water phase is used to prepare an oil-in-water emulsion. After the water phase active substance is dissolved in the second hot water phase, it is mixed with the oil-in-water emulsion. In this process, the proportion of the hot water phase and the feeding rate have a significant effect on the particle size of the emulsion. The final screening result is: based on the total mass of water added to the anti-aging microemulsion composition, the mass percentage of the first hot water phase is 2% (0.4 parts by mass), and it is slowly added to the oil phase at a rate of 50s / drop. The screening process and results are as follows:

[0117] Ⅰ: 2% hot water ratio and feeding speed screening:

[0118] Based on the dosage in Table 1, while fixing other factors, the proportion of the first hot water phase slowly added in the 20 parts by mass of the hot water phase was adjusted to 2% and 5% of the total hot water phase, respectively. The hot water proportion screening was carried out using "particle size, skin feel, and stability of the emulsion" as the screening criteria.

[0119] Based on the dosage in Table 1, keep other factors fixed, change the feeding speed of 2% hot water in 20 parts by mass of hot water phase to 40s / drop and 50s / drop respectively, and use "particle size, skin feel and stability of the emulsion" as the screening criteria to conduct hot water ratio screening.

[0120] The results are as follows Figure 1 As shown, Figure 1 As shown in Figure A, when the first hot water phase accounts for 2% of the total water added to the anti-aging composition, a microemulsion with small particle size and uniformity can be obtained. Figure 1As shown in Figure B, when the first hot water phase accounts for 5% of the total water added to the anti-aging composition, the control difficulty increases due to the large amount of hot water added, resulting in the uniformity of the microemulsion system being affected; Figure 2 As shown in Figure A, when the first hot water phase feed rate is 40s / drop, the microemulsion system has poor dispersibility; Figure 2 As shown in Figure B, when the first hot water phase accounts for 2% of the total water volume and the feeding rate is 50s / drop, subsequent tests are carried out.

[0121] II: Screening of feeding rate of the second hot water phase / water phase active substance mixture:

[0122] The process adopted by the anti-aging microemulsion composition is as follows: the aqueous phase active substance (β-glucan + sturgeon roe extract) is dissolved in the second hot water phase and added to the oil-in-water emulsion at a rate of 10s / drop. In this process, the initially formed oil-in-water microemulsion undergoes a phase transition process and gradually becomes an oil-in-water microemulsion. In this process, the feeding rate is very important and directly affects the size and stability of the emulsion particles.

[0123] Fixing other factors, the feeding speeds of 1s / drop and 10s / drop were tested respectively, and the "particle size, skin feel and stability of the emulsion" were used as screening criteria to determine the addition method of phase C.

[0124] The results are as follows Figure 3 As shown in Figure A, when the droplet addition speed is fast (1s / drop), the uniformity of the microemulsion particles formed is poor. Figure 3 As shown in Figure B, slow dripping (10s / drop) helps to improve the uniformity of microemulsion particles, and the particle size meets the requirement of <500nm, so 10s / drop was selected for subsequent experiments.

[0125] Example 5: Effect of gel phase stabilizer ratio and feed rate on microemulsion performance

[0126] Effects of the ratio of gel phase stabilizer (xanthan gum / ammonium acryloyldimethyltaurate / VP copolymer) and feeding rate on the stability of carrier microemulsion

[0127] In order to improve the stability of the active ingredient, it is necessary to select a carrier with a certain hardness and viscosity. Since the microemulsion particle size is small, there is a certain transdermal absorption efficiency. In order to further improve the safety of the system, a gel phase composed of macromolecular non-transdermal ingredients is selected as the carrier of the microemulsion composition to achieve effective absorption of the active ingredient while reducing irritation and improving stability.

[0128] Xanthan gum is a commonly used stabilizer for preparing gel products. It is mild and non-irritating to the skin, can improve the pick-up properties of the product, and make the product convenient for consumers to use. The molecular weight of xanthan gum is usually between 2 million and 50 million Daltons, and there is no risk of transdermal absorption. Ammonium acryloyldimethyltaurate / VP copolymer is a high molecular polymer that can provide high viscosity. In addition, the stabilizer has a soft skin feel. When it comes into contact with the skin, it is affected by the charge and is very easy to transfer water molecules, reducing the sense of heaviness and reducing the burden on the skin. These two stabilizers were selected as target raw materials, and the addition amount and feeding rate of these two gel phase stabilizers were explored through stability and appearance.

[0129] Compared with Example 1, only the gel phase ratio and the feeding process were adjusted, and the emulsifier and the active ingredient remained the same.

[0130] Table 5 Effect of gel phase ratio on microemulsion properties

[0131]

[0132] The above experiments revealed that the optimal conditions for using the gel phase stabilizer are xanthan gum: ammonium acryloyldimethyltaurate / VP copolymer = 1:3 (mass ratio), which is the combination of research content 1.

[0133] Since large molecular weight, high shearing speed or long-term excessive shearing will lead to decreased stability of the macromolecular gel stabilizer, thus affecting product stability, it is necessary to minimize the stirring shearing speed while ensuring that the microemulsion composition is fully dispersed.

[0134] By fixing other factors and changing the stirring speed when feeding the gel phase, the stirring speed parameters were explored using "particle size, stability, and ability to fully disperse the emulsion" as screening criteria. Figure 4 As shown, Figure 4 Figure A in the middle shows that when the stirring speed is 400 rpm / min, the particle sizes of the microemulsion composition are different and the uniformity is poor. Figure 4 In Figures B and C, the particle size is uniform when the stirring speed is 600 rpm / min and 700 rpm / min, respectively. Therefore, in the preparation process of the microemulsion of the present application, the stirring speed selected during the feeding process of the gel phase is 600 rpm / min to 700 rpm / min.

[0135] Example 6: In vitro efficacy test of the regulatory effect of the anti-aging microemulsion composition on adipocytes and fibroblasts

[0136] (1) Evaluation of the proliferation-promoting effect of the microemulsion composition on preadipocytes 3T3-L1 by MTT method: 3T3-L1 cells were inoculated and then treated with microemulsion compositions of different schemes for 48 hours. After removing the culture medium, 100 μL MTT (1.0 g / L) solution was added and placed in a 37°C, 5% CO 2 After culturing in the environment for 4 hours, the solution was discarded, 150 μL DMSO was added, and the mixture was placed at 37°C for 10 minutes. The absorbance A value of each well was measured at a wavelength of 570 nm.

[0137] (2) The MTT method was used to evaluate the proliferation-promoting effect of the microemulsion composition on mouse fibroblasts: L929 mouse fibroblasts with good growth status were inoculated into 96-well culture plates and cultured overnight. Then the culture medium was discarded, and samples of different concentrations were added. The cell control group was added with serum-free DMEM culture medium and cultured for 48 h to 72 h. The culture medium was discarded, 100 μL MTT (1.0 g / L) solution was added, and the cells were placed in a 37°C, 5% CO 2 After culturing in the environment for 4 hours, the solution was discarded, 150 μl DMSO was added, and the mixture was placed at 37°C for 10 minutes. The absorbance A value of each well was measured at a wavelength of 490 nm.

[0138] Calculation results:

[0139]

[0140] Wherein: V(%)—cell viability, %; A sample—absorbance of the system containing the test sample; A blank control—absorbance of the empty plate; A cell control—absorbance of the system without the test sample.

[0141] Compared with the example of research content 1, only the addition amount (parts by mass) of the three active ingredients was adjusted, and the other components remained unchanged.

[0142]

[0143] 3T3-L1 preadipocytes are a type of precursor cells that have the ability to proliferate and differentiate into mature adipocytes, and can differentiate into adipocytes to exert their functions. Sturgeon caviar is rich in nutrients, including nucleic acids, amino acids, polyunsaturated fatty acids, multiple vitamins, minerals, trace elements and other nutrients required for life. Sturgeon caviar extract can promote the differentiation of adipocytes, promote the secretion of adiponectin, maintain the youthful state of adipocytes, and then promote the synthesis of collagen and hyaluronic acid to inhibit aging. Dipalmitoyl hydroxyproline (DPHP) is an anti-wrinkle and skin-tightening cosmetic raw material synthesized from the natural product palmitic acid and the natural amino acid hydroxyproline. After entering the skin, DPHP releases hydroxyproline under the action of lipase, providing essential amino acid components for the growth of new adipocytes and promoting adipocytes to maintain a vigorous and young state. β-glucan is used as a cell function protector, which can be recognized by receptors on the membrane of immunocompetent cells and activate immunocompetent cells, helping new adipocytes to resist various exogenous mechanical and chemical stimuli, scavenge free radicals, fight against ultraviolet damage, and promote immune protection. The combined use of the three ingredients synergistically promotes regeneration and strong protection, thereby promoting fat cells to maintain a good growth state.

[0144] At the same time, the combined use of these three active ingredients can also promote the proliferation of fibroblasts. Fibroblasts can promote the synthesis of collagen and elastin, which are key components for maintaining the health of the extracellular matrix of the dermis.

[0145] In the above experiments, the combined use of dipalmitoyl hydroxyproline, β-glucan, and sturgeon roe extract had the best promoting effect on pre-adipocytes and fibroblasts, indicating that the three can synergistically promote adipocyte differentiation, promote fibroblast regeneration, and maintain the rejuvenated state of adipocytes and the extracellular matrix of the dermis. This verifies the effectiveness of the combined use of dipalmitoyl hydroxyproline, β-glucan, and sturgeon roe extract in maintaining the youthful state of the extracellular matrix and the regeneration of adipocytes.

[0146] Example 7: Human efficacy test of the anti-aging microemulsion in improving skin wrinkles, sagging and sagging

[0147] According to Example 1, an anti-aging microemulsion was prepared, and 20 subjects aged between 25 and 60 were screened. The subjects were required to have obvious fine lines, expression lines, wrinkles, sagging, and sagging on their faces. The products were used on time according to the test requirements during the entire research test period, and other functional products such as anti-wrinkle, firming, brightening, and oil control were not used. Preparations that affect the test were prohibited from being used on the test site. During the test, the subjects were prohibited from taking oral preparations that affect the test. The subjects were mainly indoor activities to avoid long-term exposure to light. The product was used twice a day, morning and evening, as required, for a test period of 28 days, and relevant indicators were measured before and after the test using VISIA-CR and DermaLab skin detectors.

[0148] 1. Test steps

[0149] (1) After receiving the samples, the subjects underwent the initial test and were reminded to use a soybean-sized amount of lotion on their faces in the morning and evening instead of lotion. Facial skin tests were performed in the first, second, third, and fourth weeks.

[0150] (2) In each round, VISIA-CR was used to collect wrinkle improvement images from the front, left side, and right side of the subject's face.

[0151] (3) In each round, the Dermalab skin tester was used to collect data on the water content, transepidermal water loss (TEWL), and elasticity (E value) of the subjects. Each point was tested 3 times and the average value was taken.

[0152] 2. The test results are as follows

[0153]

[0154]

[0155] Changes in the water content and TEWL value of the skin's stratum corneum characterize the health of the skin barrier. As we age, the skin becomes thinner, and the stratum corneum is prone to water loss, which leads to damage to the barrier function, skin sensitivity, and accelerated aging. Therefore, maintaining a healthy barrier function is also an important way to fight aging. AQUAXYL is a xylitol derivative that can improve skin desquamation and dryness and promote skin health. When the skin ages, its elasticity decreases and it becomes loose and sagging. Niacinamide can increase the level of autophagy in skin cells, keep the skin in a vigorous new state, and reduce aging and sensitivity problems caused by zombie cells. At the same time, Centella asiatica can promote blood circulation, help the skin increase nutrient supply, and help new collagen maintain a healthy state. These ingredients help the anti-aging microemulsion to exert its effects in terms of maintaining the growth environment of new cells, activating cell vitality, promoting nutrient supply, enhancing barrier function, and moisturizing.

[0156] Analysis of the test results of the subjects showed that the current microemulsion products can effectively moisturize, improve skin elasticity, reduce TEWL value, and improve the skin's barrier function.

[0157] like Figure 5As shown in the figure, it can be clearly seen from the ultrasound image that before the subjects used the product, the junction between the dermis and subcutaneous tissue was very sparse, the collagen density of the extracellular matrix of the dermis was low, and there were a large number of cavities. The boundary between the dermis and the subcutaneous fat layer was discontinuous and fluctuated greatly, and the black fat layer was obviously infiltrated, indicating that the skin was obviously loose and lacked elasticity. After using the product, the collagen density of the dermis increased, the firmness of the extracellular matrix increased, the cavities were significantly improved, the boundary between the dermis and the subcutaneous fat layer was clear and continuous, with less fluctuation, and the penetration of the black fat layer decreased, indicating that the subjects' skin firmness and fullness improved after the test. This shows that the current microemulsion can improve the structure of the junction between the fat layer and the dermis, and reduce the infiltration of fat cells into the dermis to aggravate aging.

[0158] In VISIA 3D imaging, different colors represent different shades of skin features, and blue represents the sunken parts of the skin. Before use, the color area of ​​the test subject's nasolabial folds was larger and darker. After use, the blue area decreased, indicating that the skin depression in this area was reduced, indicating that the depth of the nasolabial folds decreased and the skin became smoother. Figure 6 and Figure 7 As shown, the subject had obvious nasolabial folds before the test, but after the test, the nasolabial folds became lighter, indicating that the product has firming and anti-wrinkle effects.

[0159] The present application prepares a nano-scale anti-aging microemulsion with a particle size of less than 500nm, which has good biocompatibility and absorbency, improves the application effect of the microemulsion in the delivery of active substances, and improves its absorbency and bioavailability. The anti-aging emulsion of the present application enhances the anti-aging effect by regulating the synthesis of fat cells and the synthesis of extracellular matrix, avoids adding too many chemicals, and thus improves the safety of the product, which will provide new ideas and methods for the application of nanotechnology in anti-aging research. In the field of cosmetic technology, the anti-aging emulsion of the present application has the characteristics of small particle size, good absorbency and high safety, which will greatly improve the application effect of cosmetics in anti-aging and meet the needs of consumers for efficient, safe and natural anti-aging products. And the anti-aging microemulsion of the present application does not need to be obtained by high-pressure homogenization equipment, only stirring is required, and the production process is simple and feasible, with high application prospects.

[0160] The above is a detailed introduction to an anti-aging microemulsion composition, microemulsion, preparation method and application provided in the examples of the present application. Specific examples are used herein to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method of the present application and its core idea. At the same time, for those skilled in the art, according to the idea of ​​the present application, there will be changes in the specific implementation methods and application scopes. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. An anti-aging microemulsion composition, characterized in that: The invention comprises the following components in parts by mass: 8 to 10 parts of oil, 1 to 5 parts of emulsifier, 0.1 to 2 parts of oil phase active substance, 0.01 to 5 parts of water phase active substance, 1 to 5 parts of co-emulsifier and 15 to 25 parts of first solvent; the oil phase active substance comprises dipalmitoyl hydroxyproline; the oil comprises triethylhexanoin and isononyl isononanoate in a mass ratio of (1 to 7): (1 to 7); the emulsifier is PolyAquol2W; the water phase active substance comprises beta-glucan and sturgeon roe extract in a mass ratio of (0.01 to 0.1): (2 to 5).

2. The anti-aging microemulsion composition according to claim 1, characterized in that: The co-emulsifier includes pentylene glycol.

3. A method for preparing the anti-aging microemulsion composition according to claim 1, characterized in that: The following steps are involved: The oil phase active substance is mixed with the oil, stirred to dissolve, and an emulsifier is added. After stirring and dissolving at 80°C to 85°C, an auxiliary emulsifier is added to obtain an oil phase; The first solvent is heated to 80° C. to 85° C. as a hot water phase, wherein, based on the total mass of the first solvent, 2% to 5% of the hot water by mass of the first solvent is taken as the first hot water phase, and the remaining hot water phase is taken as the second hot water phase; The first hot water phase is added to the oil phase at a first rate to form a water-in-oil emulsion, and in the process of dripping the first hot water phase, stirring is continued at a first stirring rate; wherein the first rate is v1, the value of v1 is 50s / drop to 100s / drop, and the first stirring rate is R1, the value of R1 is 150rpm / min to 250rpm / min; The aqueous phase active substances including β-glucan and sturgeon roe extract are dissolved in the second hot water phase, and after being dissolved, they are added to the water-in-oil emulsion system at a second rate, and are continuously stirred at a second stirring rate; wherein the second rate is v2, and the value of v2 is 5s / drop to 15s / drop; the second stirring rate is R2, and the value of R2 is 150rpm / min to 250rpm / min; The mixture is stirred evenly to obtain an anti-aging microemulsion composition.

4. An anti-aging microemulsion, characterized in that: The anti-aging microemulsion composition according to claim 1 is included, wherein the mass percentage of the anti-aging microemulsion composition to the anti-aging microemulsion is 20% to 40% based on the total mass of the anti-aging microemulsion.

5. The anti-aging microemulsion according to claim 4, characterized in that: The anti-aging microemulsion also includes a gel phase, which includes, by weight, 0.01 to 0.5 parts of xanthan gum, 0.1 to 1 parts of ammonium acryloyldimethyltaurate / VP copolymer, 1 to 5 parts of AQUAXYL and 40 to 60 parts of water.

6. The anti-aging microemulsion according to claim 4, characterized in that: In parts by mass, the gel phase further comprises 1 to 3 parts of niacinamide and 0.5 to 1.5 parts of asiaticaside.

7. The anti-aging microemulsion according to claim 4, characterized in that: The gel phase further comprises 1 to 3 parts by mass of a second solvent, and the second solvent comprises glycerol.

8. A method for preparing the anti-aging microemulsion according to claim 4, characterized in that: The following steps are involved: preparing an anti-aging microemulsion composition; preparing a gel phase; The gel phase is added to the anti-aging microemulsion composition at a third rate, and stirring is continued at a third stirring rate, wherein the third rate is v3, v3 is 5s / drop to 15s / drop, and the third stirring rate is R3, R3 is 600rpm / min to 800rpm / min.

9. Use of the anti-aging microemulsion composition according to any one of claims 1 to 2, or the anti-aging microemulsion composition prepared by the preparation method according to claim 3, or the anti-aging microemulsion according to any one of claims 4 to 7, or the anti-aging microemulsion prepared by the preparation method according to claim 8 in the preparation of anti-aging products.

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