Application of dipeptide compounds in the preparation of soothing, anti-sugar and brightening cosmetics, soothing, anti-sugar and brightening emulsion and preparation method thereof

Abstract

The present invention relates to the field of cosmetics, specifically to the application of dipeptide compounds in the preparation of soothing anti-sugar brightening cosmetics and soothing anti-sugar brightening lotion and preparation method thereof. The soothing anti-sugar brightening lotion provided by the present invention uses one or more dipeptide compounds of Ser-His and Gly-His as soothing anti-sugar brightening raw material components, which can simultaneously solve the problems of skin inflammation, protein glycosylation in the skin and dull skin, with good effects, and will not cause skin contact allergies. Experiments show that both Ser-His and Gly-His have soothing anti-sugar brightening effects. The lotion prepared with Ser-His and Gly-His as soothing anti-sugar brightening raw materials is used continuously morning and evening for nearly one month for volunteers with sensitive skin and dull skin color. All subjects in the entire evaluation process had no adverse reactions, and more than half of the subjects gave a good subjective evaluation on the actual effect of the prepared lotion.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, in particular to application of a dipeptide compound in the preparation of soothing, anti-sugar and brightening cosmetics, a soothing, anti-sugar and brightening emulsion and a preparation method thereof. Background Art

[0002] When the skin is seriously disturbed by the external environment, it will promote skin inflammation, which manifests itself on the skin as seborrheic dermatitis, contact dermatitis, allergic dermatitis, hormone-dependent dermatitis, atopic dermatitis, neurodermatitis, etc. In addition, when disturbed by the outside world, the protein in the skin will be glycosylated, causing the skin to become dull and lusterless. At present, aminoguanidine hydrochloride can solve the problem of protein glycosylation; blue copper peptide can solve the corresponding skin inflammation.

[0003] Aminoguanidine hydrochloride has an anti-glycation effect, but it can easily cause skin contact allergies and therefore cannot be used in the cosmetics field. Peptides are composed of amino acids, have unique biological functions, are complex and varied, and are widely present in the human body. Compared with aminoguanidine hydrochloride, it has the advantages of being safer and more reliable, so it can be used in the cosmetics field. Therefore, it is of great significance and value to find a polypeptide compound that can simultaneously solve skin inflammation, protein glycosylation in the skin, and dull skin and can be used in the cosmetics field. Summary of the invention

[0004] In view of this, the technical problem to be solved by the present invention is to provide the application of a dipeptide compound in the preparation of soothing, anti-sugar and brightening cosmetics and a soothing, anti-sugar and brightening emulsion and a preparation method thereof. The soothing, anti-sugar and brightening emulsion provided by the present invention can simultaneously solve the problems of skin inflammation, protein glycosylation in the skin and dull skin, has good effects, and will not cause skin contact allergies.

[0005] The present invention provides the use of a dipeptide compound in the preparation of soothing, anti-sugar and brightening cosmetics;

[0006] The dipeptide compound is One or more of .

[0007] The dipeptide compound of the present invention is a compound formed by two amino acids through the formation of a peptide bond, has a simple structure and is stable, and is often used in cell culture to provide essential amino acids for cells. It is formed by the formation of peptide bonds between serine and histidine, and its molecular formula is C9H 14 N4O4, peptide sequence is Ser-His; It is formed by glycine and histidine through the formation of peptide bonds, and its molecular formula is C8H 12 N4O3, the peptide sequence is Gly-His.

[0008] Preferably, the dipeptide compound is in a mass ratio of (1-2): (1-2)

[0009]

[0010] More preferably, the dipeptide compound is in a mass ratio of 1:1

[0011] The dosage form of the soothing, anti-sugar and brightening cosmetics of the present invention is emulsion, cream, water, oil, gel or aerosol.

[0012] The dipeptide compounds described in the present invention, namely Ser-His and Gly-His, have not yet been used in cosmetics. The inventors of the present application have creatively discovered that Ser-His and Gly-His, as cosmetic raw materials, have soothing, anti-glycation and brightening effects. Applying them in the field of cosmetics can solve the skin problems of sensitive people, and at the same time improve skin condition and brighten skin tone by anti-glycation.

[0013] Among them, the soothing effect of the present invention is mainly manifested as an anti-inflammatory effect, which can reduce the content of inflammatory factors IL-6 and TNF-ɑ, and solve the problem of facial skin inflammation in sensitive people. The anti-sugar effect of the present invention is mainly manifested as an anti-glycosylation effect. Glycosylation is a non-enzymatic reaction in which the carbonyl group of a reducing sugar reacts with the amino group of a protein macromolecule to form a Schiff base. These Schiff bases are rearranged, leading to the formation of early glycosylation products. Subsequently, dehydration and rearrangement lead to the formation of carbonyl compounds. The cross-linking of these compounds ultimately leads to the production of advanced glycation end products (AGEs), thereby destroying the function of the protein. Therefore, the anti-sugar effect of the present invention can increase the AGEs inhibition rate and solve the problem of protein glycosylation in the skin. The brightening effect of the present invention is mainly manifested as the effect of brightening the skin tone, solving the problem of dull skin.

[0014] The present invention also provides a soothing, anti-sugar and brightening emulsion, which comprises, by parts by mass:

[0015] Water: 75-80 parts;

[0016] Olive oil: 9-11 parts;

[0017] Panthenol: 4-6 parts;

[0018] Olive emulsified wax: 1-3 parts;

[0019] Sodium carboxymethyl cellulose: 0.5-1.5 parts;

[0020] Citric acid: 0.005-0.015 parts;

[0021] 1,4-Butanediol: 1-3 parts;

[0022] Lysozyme: 0.1-0.5 parts;

[0023] Ergothioneine: 0.2-0.8 parts;

[0024] Dipeptide compound: 1.8-5 parts;

[0025] The dipeptide compound is One or more of .

[0026] Preferably, the dipeptide compound is in a mass ratio of (1-2): (1-2)

[0027]

[0028] More preferably, the dipeptide compound is in a mass ratio of 1:1

[0029] In some embodiments of the present invention, the soothing, anti-sugar and brightening lotion comprises: water: 75-80 parts;

[0030] Olive oil: 9-11 parts;

[0031] Panthenol: 4-6 parts;

[0032] Olive emulsified wax: 1-3 parts;

[0033] Sodium carboxymethyl cellulose: 0.5-1.5 parts;

[0034] Citric acid: 0.005-0.015 parts;

[0035] 1,4-Butanediol: 1-3 parts;

[0036] Lysozyme: 0.1-0.5 parts;

[0037] Ergothioneine: 0.2-0.8 parts;

[0038] 0.9~1.1 parts;

[0039] 0.9~1.1 parts.

[0040] In other embodiments of the present invention, the soothing, anti-sugar and brightening lotion comprises: water: 77-78 parts;

[0041] Olive oil: 9.5-10.5 parts;

[0042] Panthenol: 4.5-5.5 parts;

[0043] Olive emulsified wax: 1.5-2.5 parts;

[0044] Sodium carboxymethyl cellulose: 0.8-1.2 parts;

[0045] Citric acid: 0.008-0.012 parts;

[0046] 1,4-Butanediol: 1.5-2.5 parts;

[0047] Lysozyme: 0.2-0.3 parts;

[0048] Ergothioneine: 0.4-0.6 parts;

[0049] 0.9~1.1 parts.

[0050] 0.9~1.1 parts.

[0051] The inventors of the present application further discovered that by using one or more dipeptide compounds of Ser-His and Gly-His as soothing, anti-sugar and brightening raw material components, combined with a specific emulsion matrix formula, Ser-His and Gly-His can more fully exert the soothing, anti-sugar and brightening effects, and the resulting emulsion has a more excellent soothing, anti-sugar and brightening effect. The experiment showed that 10 volunteers with sensitive skin and dull complexion were selected, and after cleaning their faces in the morning and evening, the soothing, anti-sugar and brightening emulsion of the present invention was used on the whole face twice a day for 28 consecutive days. All subjects had no adverse reactions, and more than half of the subjects gave a good subjective evaluation of the product's skin feel, color spots, evenness, acne marks and brightening improvements.

[0052] The present invention also provides a method for preparing the soothing, anti-sugar and brightening lotion described in any of the above technical solutions, comprising the following steps: homogenizing and emulsifying olive oil, olive emulsifying wax, water, panthenol, sodium carboxymethyl cellulose, citric acid, 1,4-butanediol, a dipeptide compound, lysozyme and ergothioneine to obtain the soothing, anti-sugar and brightening lotion.

[0053] Specifically, the method comprises the following steps: homogenizing and emulsifying the oil phase material and the emulsified phase material at 70-80°C for 6-8 minutes, and keeping the temperature for 10-15 minutes; then adding a dipeptide compound, lysozyme and ergothioneine thereto at 35-45°C and mixing to obtain the soothing, anti-sugar and brightening lotion; the oil phase material comprises olive oil and olive emulsifying wax; the emulsified phase material comprises water, panthenol, sodium carboxymethyl cellulose, citric acid and 1,4-butylene glycol.

[0054] In certain embodiments of the present invention, olive oil and olive emulsified wax are mixed at 70-80° C. to obtain an oil phase material; water, panthenol, sodium carboxymethyl cellulose, citric acid and 1,4-butylene glycol are mixed at 70-80° C. to obtain an emulsified phase material; the oil phase material is added to the emulsified phase material, homogenized and emulsified at 70-80° C. for 6-8 min, and kept warm for 10-15 min; then a dipeptide compound, lysozyme and ergothioneine are added thereto at 35-45° C. and mixed for 20-50 min to obtain the soothing, anti-sugar and brightening lotion.

[0055] The present invention provides the use of dipeptide compounds in the preparation of soothing, anti-sugar and brightening cosmetics, a soothing, anti-sugar and brightening emulsion and a preparation method thereof. The soothing, anti-sugar and brightening emulsion provided by the present invention uses one or more dipeptide compounds of Ser-His and Gly-His as soothing, anti-sugar and brightening raw material components, which can simultaneously solve the problems of skin inflammation, protein glycosylation in the skin and dull skin, has good effects, and does not cause skin contact allergies. Experiments show that both Ser-His and Gly-His have soothing, anti-sugar and brightening effects. The emulsion prepared with Ser-His and Gly-His as soothing, anti-sugar and brightening raw materials was used continuously morning and evening for nearly a month for volunteers with sensitive skin and dull skin color. First of all, no adverse reactions occurred in all subjects during the entire evaluation process. In addition, more than half of the subjects gave a good subjective evaluation on the actual effect of the prepared emulsion. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 This is a data graph showing the effect of Ser-His on the inflammatory factor IL-6;

[0057] Figure 2 This is a data chart showing the effect of Ser-His on the inflammatory factor TNF-ɑ;

[0058] Figure 3 The data diagram is the inhibitory effect of Ser-His on AGEs;

[0059] Figure 4 This is a data graph showing the effect of Gly-His on the inflammatory factor IL-6;

[0060] Figure 5 This is a data chart showing the effect of Gly-His on the inflammatory factor TNF-ɑ;

[0061] Figure 6 This is a data diagram of the inhibitory effect of Gly-His on AGEs;

[0062] Figure 7 This is a data graph showing the inhibitory effect of His on AGEs. DETAILED DESCRIPTION

[0063] The present invention discloses the use of dipeptide compounds in the preparation of soothing, anti-sugar and brightening cosmetics, a soothing, anti-sugar and brightening emulsion and a preparation method thereof. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It is particularly important to point out that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the method and application of this article without departing from the content, spirit and scope of the present invention to realize and apply the technology of the present invention.

[0064] The basic information of the dipeptide used in the present invention is shown in Table 1:

[0065] Table 1 Basic information of dipeptides

[0066]

[0067] Among them, Ser-His and Gly-His were prepared in-house and synthesized by solid phase method, as follows:

[0068] Synthesis of Ser-His: First, polystyrene resin was used as a solid phase carrier, and His (Fmoc-His(Trt)-OH) modified with a protective group was fixed on the resin, reacted at room temperature for 2 hours, and the Fmoc protective group on His was removed using a 20% pyridine / DMF solution; Fmos-Ser(tBu)-OH was added, condensed under the action of a condensing agent HBTU / HOBt, reacted at room temperature for 2 hours, and the Fmoc protective group on Ser was removed using a 20% pyridine / DMF solution. Finally, trifluoroacetic acid solution was used to remove the tBu side chain protective group of Ser and the resin.

[0069] Synthesis of Gly-His: First, polystyrene resin was used as a solid phase carrier, and His (Fmoc-His(Trt)-OH) modified with a protective group was fixed on the resin, reacted at room temperature for 2 hours, and the Fmoc protective group on His was removed using a 20% pyridine / DMF solution; Fmos-Gly(tBu)-OH was added, condensed under the action of a condensing agent HBTU / HOBt, reacted at room temperature for 2 hours, and the Fmoc protective group on Gly was removed using a 20% pyridine / DMF solution. Finally, trifluoroacetic acid solution was used to remove the tBu side chain protective group of Gly and the resin.

[0070] Mouse macrophages (RAW264.7) were from Wuhan Pronocell Life Science Co., Ltd.;

[0071] Lipopolysaccharide (LPS) was from Beijing Solebow Technology Co., Ltd.;

[0072] RAW264.7 special culture medium comes from Wuhan Pronocell Life Science Co., Ltd.;

[0073] Mouse tumor necrosis factor α (TNF-α) enzyme-linked immunosorbent assay kit was from Wuhan Elaruite Biotechnology Co., Ltd.;

[0074] Mouse interleukin-6 (IL-6) enzyme-linked immunosorbent assay kit was from Wuhan Elaruite Biotechnology Co., Ltd.;

[0075] DPPH (1,1-diphenyl-2-picrylhydrazyl free radical) reagent was from Beijing Solebow Technology Co., Ltd.;

[0076] BSA (bovine serum albumin) was from Shanghai Bio-Tech Biotechnology Co., Ltd.;

[0077] Glucose was from Shanghai Bio-Tech Biotechnology Co., Ltd.

[0078] The present invention will be further described below in conjunction with embodiments:

[0079] Example 1

[0080] To prove that Ser-His has anti-inflammatory effects and can reduce the content of inflammatory factors IL-6 and TNF-ɑ, the following experiment was designed and conducted. Experimental process: 2 million RAW264.7 cells (P9) were plated in each well of a 6-well plate. After 24 hours, 2 mL of dexamethasone (DEX, 100 μg / mL) or Ser-His (1000 μg / mL) was added. After 1.5 hours, 2 μL of 1000 μg / mL LPS was added except for the Control group. Continue to culture for 24 hours, collect the supernatant, centrifuge, take the supernatant, dilute 200 times for TNF-α experiment and 50 times for IL-6 experiment, and then use mouse tumor necrosis factor α (TNF-α) enzyme-linked immunosorbent assay kit and mouse interleukin 6 (IL-6) enzyme-linked immunosorbent assay kit to detect the concentration of inflammatory factors TNF-α and IL-6, respectively. Within 15 minutes after adding the stop solution, detect its absorbance at 450 nm. The specific groups are shown in Table 2 below. Table 2 is the anti-inflammatory experiment grouping table:

[0081] Table 2

[0082] Group system Control Group Culture medium Negative control group Culture medium + 1 μg / mL LPS Positive control group Culture medium + 1 μg / mL LPS + 100 μg / mL dexamethasone Ser-His group Culture medium + 1 μg / mL LPS + 1000 μg / mL Ser-His

[0083] Experimental results: The experimental results are as follows Figure 1-2 As shown, Figure 1 This is a data chart showing the effect of Ser-His on the inflammatory factor IL-6. Figure 2 The data of the effect of Ser-His on inflammatory factor TNF-ɑ is shown in Figure 2. Figure 1 and Figure 2It is known that lipopolysaccharide (LPS) can promote inflammation, produce a large number of inflammatory factors and promote the expression of related inflammatory proteins. LPS-activated mouse leukemia macrophages (RAW264.7) are often used as a cell inflammation model. Compared with the negative control group, Ser-His can reduce the concentration of IL-6 and TNF-ɑ, and it is statistically significant (P<0.05 is considered statistically significant, "**" represents P<0.01;

[0084] “***” represents P<0.001; “****” represents P<0.0001).

[0085] In order to prove that Ser-His has an anti-glycemic effect and can reduce the level of protein glycosylation, the following experiment was designed and conducted. Experimental principle: Glycosylation is a non-enzymatic reaction in which the carbonyl group of a reducing sugar reacts with the amino group of a protein macromolecule to form a Schiff base. These Schiff bases are rearranged, leading to the formation of early glycosylation products. Subsequently, dehydration and rearrangement lead to the formation of carbonyl compounds. The cross-linking of these compounds ultimately leads to the production of advanced glycation end products (AGEs), which destroy the function of the protein. AGEs will spontaneously fluoresce (Ex: 370nm, Em: 440nm), so the level of glycosylation can be reflected by detecting the fluorescence intensity.

[0086] Experimental process: PBS was used to prepare 9000ppm carnosine solution, 9000ppm Ser-His solution, 9000ppm Gly-His solution, 30mg / mL BSA, and 1.5M high glucose solution; the reaction system was prepared: 1mL of 30mg / mL BSA, 1mL of 1.5M glucose solution, 1mL of 9000ppm carnosine solution, Ser-His solution, and Gly-His solution were reacted in a 60℃ water bath for 48 hours; 200μL of the reaction solution was taken in a 96-well black well plate, and the fluorescence intensity of each group was detected in an ELISA reader (Ex: 370nm, Em: 440nm). The groups are shown in Table 3 below:

[0087] Table 3

[0088] Group system Final sample concentration Control Group BSA+Glucose+PBS - Positive control group BSA+Glucose+Carnosine 3000 Ser-His group BSA+Glucose+Ser-His 3000

[0089] Experimental results: The experimental results are as follows Figure 3 As shown, Figure 3 Figure 2 is the data of the inhibitory effect of Ser-His on AGEs. Figure 3 It can be seen that compared with the Control group, the advanced glycation end products (AGEs) in the Ser-His group decreased. This shows that Ser-His can inhibit the glycosylation reaction of proteins. Compared with the Control group, the Ser-His group inhibited the content of AGEs, and it was statistically significant (P<0.05 was considered statistically significant,

[0090] “**” represents P<0.01; “****” represents P<0.0001).

[0091] Example 2

[0092] To prove that Gly-His has anti-inflammatory effects and can reduce the content of inflammatory factors IL-6 and TNF-ɑ, the following experiments were designed and conducted. Experimental process: 2 million RAW264.7 cells (P9) were plated in each well of a 6-well plate, and 2 mL of dexamethasone (DEX, 100 μg / mL) or Gly-His (1000 μg / mL) was added after 24 hours. After 1.5 hours, 2 μL of 1000 μg / mL LPS was added except for the Control group. Continue to culture for 24 hours, collect the supernatant, centrifuge, take the supernatant, dilute 200 times for TNF-α experiment and 50 times for IL-6 experiment, and then use the mouse tumor necrosis factor α (TNF-α) enzyme-linked immunosorbent assay kit and mouse interleukin 6 (IL-6) enzyme-linked immunosorbent assay kit to detect the concentrations of inflammatory factors TNF-α and IL-6, respectively. Add the stop solution within 15 minutes and detect its absorbance at 450 nm. The specific groups are shown in Table 4 below:

[0093] Table 4

[0094]

[0095]

[0096] Experimental results: The experimental results are as follows Figure 4-5 As shown, Figure 4 This is the data chart of the effect of Gly-His on the inflammatory factor IL-6. Figure 5 The data of the effect of Gly-His on inflammatory factor TNF-ɑ is shown in Figure 2. Figure 4 and Figure 5 It is known that lipopolysaccharide (LPS) can promote inflammation, produce a large number of inflammatory factors and promote the expression of related inflammatory proteins. LPS-activated mouse leukemia macrophages (RAW264.7) are often used as a cell inflammation model. Compared with the negative control group, Gly-His can reduce the concentration of IL-6 and TNF-ɑ, and it is statistically significant (P<0.05 is considered statistically significant, "*" represents P<0.05; "**" represents P<0.05).

[0097] represents P<0.01; “****” represents P<0.0001).

[0098] In order to prove that Gly-His has an anti-glycemic effect and can reduce the level of protein glycosylation, the following experiment was designed and conducted. Experimental principle: Glycation is a non-enzymatic reaction in which the carbonyl group of a reducing sugar reacts with the amino group of a protein macromolecule to form a Schiff base. These Schiff bases are rearranged, leading to the formation of early glycosylation products. Subsequently, dehydration and rearrangement lead to the formation of carbonyl compounds. The cross-linking of these compounds ultimately leads to the production of advanced glycation end products (AGEs), which destroy the function of the protein. AGEs will spontaneously fluoresce (Ex: 370nm, Em: 440nm), so the level of glycosylation can be reflected by detecting the fluorescence intensity.

[0099] Experimental process: PBS was used to prepare 9000ppm carnosine solution, 9000ppm carnosine solution, 9000ppm Gly-His solution, 30mg / mL BSA, and 1.5M high glucose solution; the reaction system was prepared: 1mL of 30mg / mL BSA, 1mL of 1.5M glucose solution, 1mL of 9000ppm carnosine solution, and Gly-His solution were reacted in a 60℃ water bath for 48 hours; 200μL of the reaction solution was taken in a 96-well black well plate, and the fluorescence intensity of each group was detected in an ELISA reader (Ex: 370nm, Em: 440nm). The groups are shown in Table 5 below:

[0100] Table 5

[0101] Group system Final sample concentration Control Group BSA+Glucose+PBS - Positive control group BSA+Glucose+Carnosine 3000 Gly-His group BSA+Glucose+Gly-His 3000

[0102] Experimental results: Figure 6 As shown, Figure 6 The data of the inhibitory effect of Gly-His on AGEs is shown in Figure 2. Figure 6 It can be seen that compared with the Control group, the advanced glycation end products (AGEs) in the Gly-His group decreased. This shows that Gly-His can inhibit the glycosylation reaction of proteins. Compared with the Control group, the Gly-His group inhibited the content of AGEs, and it was statistically significant (P<0.05 was considered statistically significant, "**"

[0103] represents P < 0.01).

[0104] Comparative Example 1

[0105] His has an anti-sugar effect and can reduce the level of protein glycosylation. Experimental principle: Glycosylation is a non-enzymatic reaction in which the carbonyl group of a reducing sugar reacts with the amino group of a protein macromolecule to form a Schiff base. These Schiff bases rearrange, leading to the formation of early glycosylation products. Subsequently, dehydration and rearrangement lead to the formation of carbonyl compounds. The cross-linking of these compounds ultimately leads to the production of advanced glycation end products (AGEs), which destroy the function of the protein. AGEs will spontaneously fluoresce (Ex: 370nm, Em: 440nm), so the level of glycosylation can be reflected by detecting the fluorescence intensity.

[0106] Experimental process: PBS was used to prepare 9000ppm carnosine solution, 9000ppm His solution, 30mg / mL BSA, and 1.5M high glucose solution; the reaction system was prepared: 1mL of 30mg / mL BSA, 1mL of 1.5M glucose solution, 1mL of 9000ppm carnosine solution, and His solution was reacted in a 60℃ water bath for 48 hours; 200μL of the reaction solution was taken in a 96-well black well plate, and the fluorescence intensity of each group was detected in an ELISA reader (Ex: 370nm, Em: 440nm). The groups are shown in Table 6 below:

[0107] Table 6

[0108]

[0109]

[0110] Experimental results: The experimental results are as follows Figure 7 As shown, Figure 7 The data of His inhibitory effect on AGEs are shown in Figure 2. Figure 7 It can be seen that compared with the Control group, the advanced glycation end products (AGEs) in the His group decreased. This shows that His can inhibit the glycosylation reaction of proteins. Compared with the Control group, the His group inhibited the content of AGEs, and it was statistically significant (P<0.05 is considered statistically significant, "**" represents P<0.01; "****" represents P<0.0001).

[0111] Example 3

[0112] Take each component according to the amount in Table 7, including the following steps:

[0113] (1) Add olive oil and olive emulsified wax into the oil phase pot, stir and heat to 75°C until they are completely dissolved;

[0114] (2) Add 30 parts of water, panthenol, sodium carboxymethyl cellulose, citric acid, and 1,4-butylene glycol into an emulsifying pot, stir and heat to 75°C until completely dissolved;

[0115] (3) The material in the oil phase pot is pumped into the emulsification pot, homogenized and emulsified for 8 minutes, kept warm for 10 minutes and then cooled;

[0116] (4) When the temperature drops to 40°C, add the sterilized Ser-His / Gly-His / lysozyme / ergothioneine to the emulsifier and stir for 30 minutes; stir for 30 minutes in the emulsifier;

[0117] (5) After the sampling inspection is passed, the material is discharged.

[0118] Table 7

[0119]

[0120]

[0121] 10 volunteers with sensitive skin and dull complexion were selected. After cleansing their faces in the morning and evening, the soothing, anti-sugar and brightening essence in Example 3 was applied to their entire faces 2 times / d for 28 consecutive days.

[0122] Subjective evaluation of the subjects: After 28 days of use, the subjects were evaluated subjectively, including the improvement of five aspects: skin feel, brightening, evenness, spots, and acne marks. The evaluation criteria are divided into three levels: good, general, and poor. In addition, during the follow-up visit for product safety evaluation, the subjects were evaluated for adverse reactions and asked whether they had symptoms such as erythema and tingling on their faces. If the above symptoms occurred, a patch test was performed to evaluate the correlation with the test product.

[0123] Subjective evaluation of the subjects After 28 days of use, the subjects conducted a subjective evaluation, which included improvements in five aspects: skin feel, brightening, evenness, pigmentation, and acne scars. See Table 8 for details.

[0124] Table 8

[0125] project good(%) generally(%) Poor (%) Product skin feel 50 40 10 Pigmentation 60 30 10 uniform 70 30 0 Acne scars 60 30 10 Brighten 80 20 0

[0126] Product Safety Evaluation During the entire research process, no adverse reactions occurred in the subjects, proving that the soothing, anti-sugar and brightening essence has a high level of safety.

[0127] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. Application of dipeptide compounds in the preparation of soothing, anti-sugar and brightening cosmetics; The dipeptide compound is One or more of .

2. The use according to claim 1, characterized in that: The dipeptide compound is a compound having a mass ratio of (1-2): (1-2) 3. The use according to claim 1, characterized in that: The dipeptide compound is a 1:1 mass ratio 4. The use according to claim 1, 2 or 3, characterized in that: The dosage form of the soothing, anti-sugar and brightening cosmetic is emulsion, cream, water, oil, gel or aerosol.

5. Soothing anti-sugar brightening lotion, characterized by: In parts by mass, including: Water: 75-80 parts; Olive oil: 9-11 parts; Panthenol: 4-6 parts; Olive emulsified wax: 1-3 parts; Sodium carboxymethyl cellulose: 0.5-1.5 parts; Citric acid: 0.005-0.015 parts; 1,4-Butanediol: 1-3 parts; Lysozyme: 0.1-0.5 parts; Ergothioneine: 0.2-0.8 parts; Dipeptide compound: 1.8-5 parts; The dipeptide compound is One or more of .

6. The soothing, anti-sugar and brightening lotion according to claim 5, characterized in that: The dipeptide compound is a compound having a mass ratio of (1-2): (1-2) 7. The soothing, anti-sugar and brightening lotion according to claim 5, characterized in that: The dipeptide compound is a 1:1 mass ratio 8. The soothing, anti-sugar and brightening lotion according to any one of claims 5 to 7, characterized in that: include: Water: 75-80 parts; Olive oil: 9-11 parts; Panthenol: 4-6 parts; Olive emulsified wax: 1-3 parts; Sodium carboxymethyl cellulose: 0.5-1.5 parts; Citric acid: 0.005-0.015 parts; 1,4-Butanediol: 1-3 parts; Lysozyme: 0.1-0.5 parts; Ergothioneine: 0.2-0.8 parts; 0.9~1.1 parts; 0.9~1.1 parts.

9. The soothing, anti-sugar and brightening lotion according to claim 8, characterized in that: include: Water: 77-78 parts; Olive oil: 9.5-10.5 parts; Panthenol: 4.5-5.5 parts; Olive emulsified wax: 1.5-2.5 parts; Sodium carboxymethyl cellulose: 0.8-1.2 parts; Citric acid: 0.008-0.012 parts; 1,4-Butanediol: 1.5-2.5 parts; Lysozyme: 0.2-0.3 parts; Ergothioneine: 0.4-0.6 parts; 0.9~1.1 parts; 0.9~1.1 parts.

10. The method for preparing the soothing, anti-sugar and brightening lotion according to any one of claims 5 to 9, characterized in that: The following steps are involved: Olive oil, olive emulsifying wax, water, panthenol, sodium carboxymethyl cellulose, citric acid, 1,4-butylene glycol, dipeptide compound, lysozyme and ergothioneine are homogenized and emulsified to obtain the soothing, anti-sugar and brightening lotion.

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