Whitening and freckle-removing active composition, preparation method and application of whitening and freckle-removing active composition in cosmetics

Abstract

The invention relates to the technical field of biological medicine, in particular to a whitening and freckle-removing active composition, a preparation method and application of the whitening and freckle-removing active composition in cosmetics, the whitening and freckle-removing active composition comprises rhodiola crenulata root extract, mulberry (MORUS ALBA) extract and hexapeptide-2, the whitening and freckle-removing active composition has a multi-target-point effect, all the components are reasonably matched, and the whitening and freckle-removing active composition can be used for whitening and removing freckles. The obvious synergistic effect can be achieved on the antioxidant effect, the whitening effect and the freckle removing effect.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to a whitening and freckle-removing active composition, a preparation method and application thereof in cosmetics. Background Art

[0002] With the improvement of people's living standards and the pursuit of beauty, it is becoming more and more common for the skin to lose its bright whiteness or even develop spots due to factors such as ultraviolet radiation, genetics, bad living habits, and aging. The market demand for whitening and freckle removal products continues to increase.

[0003] Skin color is mainly determined by the content of melanin in the skin. Skin melanin is a ubiquitous biological pigment. The synthesis, transport and metabolism of melanin in melanocytes are complex biochemical processes involving multiple enzymes. Skin whitening needs to start from melanin synthesis, melanosome transport, metabolism, oxidative stress, ultraviolet stimulation and other environmental stimuli to achieve the desired effect. Skin whitening is mainly carried out from the following three aspects: inhibiting the synthesis of melanin, regulating the melanin metabolic pathway, and promoting melanin metabolism. Many ingredients with whitening and freckle removal effects have been discovered and applied one after another, such as niacinamide, tranexamic acid, and phenethyl resorcinol, which can inhibit the activity of tyrosinase. At the same time, natural plant extracts with antioxidant effects and salicylic acid that promotes keratin renewal are also used to accelerate melanin metabolism.

[0004] As stated in the background technology section of the Chinese invention patent with document number CN118717601A, it has been found through years of research and use that it is difficult for a single whitening active raw material to achieve a good whitening effect, and most whitening active raw materials are extremely unstable while having high whitening activity. Therefore, the development of current whitening products requires the selection of corresponding whitening efficacy raw materials based on multiple whitening target mechanisms, and the reasonable combination of whitening raw materials based on the understanding of their physical and chemical properties and safety, and the verification from the perspective of safety and efficacy, so as to truly develop effective, mild and safe whitening products to truly meet consumers' demand for whitening. Summary of the invention

[0005] In order to solve the above technical problems, the purpose of the present invention is to provide a whitening and freckle-removing active composition, a preparation method and its application in cosmetics. The whitening and freckle-removing active composition has multi-target effects, and the components are reasonably matched, which can play an obvious synergistic role in antioxidant effect and whitening and freckle-removing effect.

[0006] In order to achieve the above technical effects, the present invention adopts the following technical solutions: In a first aspect, the present invention provides a whitening and freckle-removing active composition, which comprises the following raw materials: Rhodiola rosea (RHODIOLA CRENULATA) root extract and MORUS ALBA (MORUS ALBA) extract.

[0007] Preferably, the whitening and anti-freckle active composition further comprises the following raw materials: hexapeptide-2.

[0008] Preferably, the whitening and freckle-removing active composition comprises the following raw materials in parts by weight: 2 to 15 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 1 to 8 parts of MORUS ALBA (MORUS ALBA) extract.

[0009] Furthermore, the whitening and anti-freckle active composition also includes the following raw materials in parts by mass: 4 parts of hexapeptide-2.

[0010] Preferably, in the whitening and freckle-removing active composition provided by the present invention, the MORUS ALBA extract is any one of a MORUS ALBA leaf extract, a MORUS ALBA branch extract or a MORUS ALBA root bark extract.

[0011] In a second aspect, the present invention further provides a method for preparing a whitening and anti-freckle active composition, which specifically comprises the following steps: The root extract of Rhodiola rosea (RHODIOLA CRENULATA) was prepared by water extraction or alcohol extraction.

[0012] Preferably, the preparation method comprises the following steps: Mulberry (MORUS ALBA) leaf extract was prepared by fermentation method.

[0013] Further preferably, the fermentation method is: S1: Select fresh, pest-free mulberry leaves, dry them to a moisture content of 60% to 70%, and then cut them; S2: Mix the cut mulberry leaves with fermentation liquid at an inoculation ratio of 3% to 10%, wherein the fermentation liquid is 3% to 10% of the mass of the mulberry leaves, and stir and mix thoroughly so that the microorganisms in the fermentation liquid are evenly attached to the mulberry leaves, and ferment at 30°C to 37°C for 3 to 9 days; S3: After the fermentation is completed, the fermented mulberry leaves are boiled and extracted at a solid-liquid ratio of 1:20-50, the extraction time is 1-4 hours, the extraction is performed 2-3 times, and the extracts from multiple extractions are mixed. After mixing, the filtrate is collected by filtration and freeze-dried to obtain a mulberry (MORUS ALBA) leaf extract.

[0014] More preferably, the fermentation broth contains Candida aeruginosa, Bacillus cereus and Aspergillus oryzae, and the ratio of the number of live bacteria of the three species is 2-3:4-6:1-3.

[0015] In a third aspect, the present invention also provides a use of the aforementioned whitening and anti-freckle active composition in skin care products and cosmetics.

[0016] Compared with the prior art, the present invention has the following beneficial effects: First, the composition provided by the present invention includes Rhodiola rosea root extract, MORUS ALBA leaf extract and hexapeptide-2, wherein the Rhodiola rosea root extract is rich in salidroside, tyrosol and other ingredients, has the ability of anti-oxidation and anti-free radical, can reduce the damage of ultraviolet rays and other factors to skin cells, and inhibit the production of melanin from the root. The flavonoids and phenolic compounds in the mulberry extract also have the effects of anti-oxidation and inhibiting tyrosinase activity, and can reduce melanin synthesis. Hexapeptide-2, as an antagonist of α-melanocyte stimulating hormone (α-MSH) receptor, can block the binding of α-MSH to its receptor, reduce tyrosinase activity, and further inhibit melanin production. The three ingredients work synergistically, exerting whitening and freckle removal effects from multiple targets, and the effect is more significant than that of a single ingredient. Secondly, the Rhodiola rosea root extract and mulberry extract in the composition of the present invention are both derived from natural plants, and the nature of the natural ingredients is relatively mild, while exerting the whitening and freckle removal effects, reducing irritation and damage to the skin. Even for sensitive skin, it has a high tolerance, reducing the probability of adverse reactions such as allergies. Finally, the composition provided by the present invention also uses a specific fermentation method to prepare Morusalba leaf extract, using Candida albicans, Bacillus cereus and Aspergillus oryzae to ferment according to a specific ratio of viable bacteria, which can not only increase the content and activity of the effective ingredients in the extract, but also make the extract more mild, further enhancing the safety of the product. In summary, the whitening and freckle-removing active composition has a broad market application prospect. DETAILED DESCRIPTION

[0017] The following examples are only used to more clearly illustrate the technical solutions of the present invention, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present invention. The specific embodiments listed in the present invention are only examples of the present invention, and the present invention is not limited to the specific embodiments described below. For those skilled in the art, any equivalent modifications and substitutions made to the embodiments described below are also within the scope of the present invention. Therefore, the equalization changes and modifications made without departing from the spirit and scope of the present invention should be included in the scope of the present invention.

[0018] In order to better illustrate the present invention, numerous specific details are provided in the following specific embodiments. It should be understood by those skilled in the art that the present invention can be implemented without certain specific details. In other embodiments, methods, means, equipment and steps well known to those skilled in the art are not described in detail in order to highlight the subject matter of the present invention.

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those of ordinary skill in the art. Unless otherwise specified, the units used in this specification are international standard units, and the numerical values ​​and numerical ranges appearing in the present invention should be understood to include inevitable systematic errors in industrial production. Unless otherwise specified, the experimental methods used in the following implementations are all conventional methods; the materials, reagents or instruments used without indicating the manufacturer are all reagents and materials available from commercial channels. Those without indicating specific conditions in the examples are all carried out according to conventional conditions or conditions recommended by the manufacturer. At the same time, the present invention does not limit the source of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are all common commercial products in the field of this technology.

[0020] It should be noted that the raw materials and equipment used in the specific embodiments of the present invention are all known products, which are obtained by purchasing commercial products. Among them, the preparation process of the modern Chinese medicine preparation made from the Chinese medicine composition of the present invention is mature and can be prepared by referring to the conventional methods of the prior art.

[0021] Example 1 This embodiment provides a whitening and freckle-removing active composition, which includes the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. At the same time, the present invention also provides a preparation method thereof, specifically: A1: Preparation of Rhodiola rosea root extract: The root extract of Rhodiola rosea (RHODIOLA CRENULATA) is prepared by water extraction, specifically: first rinse the root of Rhodiola rosea with clean water to remove impurities and dust, and then cut it into small segments, each of which is about 1 to 2 cm long. The cut root segments are placed in a container and extracted according to a solid-liquid ratio of 1:30, the extraction time is 30 min, and the extraction is performed twice in total. After the extraction, the extracts are combined and the residue is removed to obtain the water extract of the root of Rhodiola rosea. The water extract is concentrated and dried under reduced pressure to obtain the root extract of Rhodiola rosea (RHODIOLA CRENULATA).

[0022] A2: Preparation of MULUS ALBA LEAF EXTRACT: S1: Select fresh, pest-free mulberry leaves, dry them to a moisture content of 60% to 70%, and then cut them; S2: Mix the cut mulberry leaves with fermentation liquid, the inoculation ratio is 5%, the fermentation liquid is 3% to 10% of the mass of the mulberry leaves, and stir and mix thoroughly to make the microorganisms in the fermentation liquid evenly adhere to the mulberry leaves, and ferment at 34°C for 5 days. The strain contained in the fermentation liquid is Candida aeruginosa, and the concentration of the fermentation liquid is 1*10 5 CFU / mL.

[0023] S3: After the fermentation is completed, the fermented mulberry leaves are boiled and extracted at a solid-liquid ratio of 1:30 for 2 hours, and the extraction is performed twice. The extracts from the two extractions are mixed, and the filtrate is collected by filtration after mixing and freeze-dried to obtain a mulberry leaf extract.

[0024] Example 2 This embodiment provides a whitening and freckle-removing active composition, which is different from Embodiment 1 in that the preparation method of the root extract of Rhodiola rosea (RHODIOLA CRENULATA) used in the present invention adopts an alcohol extraction method, specifically: With reference to the aforementioned embodiment 1, the difference between this embodiment and embodiment 1 is that: Wash and cut the roots of Rhodiola rosea into small pieces, put them into a container, and add 95% by volume of The ethanol-water solution is used for extraction, and ultrasonic extraction is performed after soaking for 12 hours at a solid-liquid ratio of 1:30. The ultrasonic power is usually 500 W, and the extraction time is 25 minutes. After the extraction, the extract is concentrated and dried to obtain the Rhodiola rosea root extract.

[0025] Example 3 This embodiment provides a whitening and freckle-removing active composition, which includes the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. At the same time, the present invention also provides a preparation method thereof, which specifically includes the steps of: A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared by referring to Example 2; A2: Preparation of MORUS ALBA leaf extract. The difference between the preparation method and that of Example 2 is that the concentration of the fermentation liquid is 1*10 5 CFU / mL, the bacteria contained in the fermentation broth were Candida aeruginosa and Bacillus cereus, and the ratio of the number of live bacteria between the two was 2:4.

[0026] Example 4

[0027] This embodiment provides a whitening and freckle-removing active composition, which includes the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. At the same time, the present invention also provides a preparation method thereof, which specifically includes the steps of: A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared by referring to Example 2; A2: Preparation of MORUS ALBA leaf extract. The difference between the preparation method and that of Example 2 is that the concentration of the fermentation liquid is 1*10 5 CFU / mL, the bacteria contained in the fermentation broth were Candida albicans and Aspergillus niger, and the ratio of the number of live bacteria between the two was 2:1.

[0028] Example 5 This embodiment provides a whitening and freckle-removing active composition, which includes the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. At the same time, the present invention also provides a preparation method thereof, which specifically includes the steps of: A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared by referring to Example 2; A2: Preparation of MORUS ALBA leaf extract. The difference between the preparation method and that of Example 2 is that the concentration of the fermentation liquid is 1*10 5 CFU / mL, the bacteria contained in the fermentation broth were Candida albicans and Aspergillus oryzae, and the ratio of the number of live bacteria between the two was 2:1.

[0029] Example 6 This embodiment provides a whitening and freckle-removing active composition, which includes the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. At the same time, the present invention also provides a preparation method thereof, which specifically includes the steps of: A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared by referring to Example 2; A2: Preparation of MORUS ALBA leaf extract. The difference between the preparation method and that of Example 2 is that the concentration of the fermentation liquid is 1*10 5 CFU / mL, the bacterial species contained in the fermentation broth were Candida aeruginosa, Bacillus cereus and Aspergillus oryzae, and the ratio of the number of live bacteria among the three was 2:4:1.

[0030] Example 7 This embodiment provides a whitening and freckle-removing active composition, which includes the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. At the same time, the present invention also provides a preparation method thereof, which specifically includes the steps of: A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared by referring to Example 2; A2: Preparation of MORUS ALBA leaf extract. The difference between the preparation method and that of Example 2 is that the concentration of the fermentation liquid is 1*10 5 CFU / mL, the bacterial species contained in the fermentation broth were Candida aeruginosa, Bacillus cereus and Aspergillus niger, and the ratio of the number of live bacteria among the three was 2:4:1.

[0031] Example 8 This embodiment provides a whitening and freckle-removing active composition, which includes the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA branch extract. At the same time, the present invention also provides a preparation method thereof, which specifically includes the steps of: A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared by referring to Example 2; A2: Prepare mulberry (MORUS ALBA) branch extract. The preparation method thereof is similar to that of Example 2, but the difference from Example 1 is that the raw material used in this example is mulberry branch.

[0032] Test Example 1 Based on Examples 1 to 8, the present invention conducted the following tests on the whitening and anti-freckle active compositions prepared in the aforementioned Examples (recorded as Composition 1 to Composition 8, respectively): 1.1 DPPH clearance rate DPPH is a stable free radical. When an antioxidant is present, it can react with DPPH to change the color of DPPH from dark purple to light yellow. The scavenging rate of DPPH free radicals can be calculated by measuring the change in absorbance. Therefore, this example tests compositions 1 to 8 with reference to the DPPH scavenging rate test method in GB / T 39100-2020. The specific method will not be repeated here. Each group of experiments was repeated three times. The experimental results are shown in Table 1.

[0033] 1.2 Inhibitory activity against tyrosinase Tyrosinase is a key enzyme in the biosynthesis of melanin in the skin. Tyrosinase can catalyze the conversion of dopa into dopaquinone in a phosphoric acid solution at pH 6.8, and the absorbance can be measured at 475 nm on a spectrophotometer. Raw materials with tyrosinase activity inhibition can reduce the conversion of dopa into dopaquinone, thereby reducing the absorbance value. According to the change in absorbance value, it can be used to evaluate the inhibitory effect of cosmetic raw materials on tyrosinase activity. Therefore, this example tests the inhibitory activity of compositions 1 to 8 on tyrosinase with reference to "T / SHRH 015-2018", and the experimental results are shown in Table 1.

[0034] 1.3 Effect on melanin content in B16-F10 cells B16-F10 cells were inoculated into culture dishes or culture flasks, cultured in DMEM medium containing 10% fetal bovine serum in an incubator at 37°C and 5% CO2, and used in experiments when the cells grew to the logarithmic growth phase.

[0035] The experimental groups used Composition 1 to Composition 8, and a blank control group was set up at the same time. The treatment solution of Composition 1 to Composition 8 was added to each experimental group. After incubation for 24 hours, the culture medium was discarded and the cells were rinsed with PBS 2 to 3 times. Cell lysis solution was added and incubated at room temperature for 2 hours to fully lyse the cells and release melanin. The cell lysate was transferred to a centrifuge tube and centrifuged to take the supernatant. The absorbance of the supernatant was measured at a wavelength of 475nm by spectrophotometry, and then the relative change of melanin content in each experimental group relative to the blank control group was calculated. The experimental results are shown in Table 1.

[0036] Table 1 Test results of compositions 1 to 8 DPPH removal rate (%) Tyrosinase inhibitory activity (%) Melanin relative content (%) Example 1 56.41±2.11 35.34±1.21 -21.07 Example 2 61.02±1.97 44.08±0.96 -34.12 Example 3 61.29±2.33 56.31±3.04 -33.43 Example 4 63.82±3.54 53.55±2.01 -41.02 Example 5 61.09±2.08 58.24±3.31 -32.19 Example 6 91.61±2.90 89.37±1.13 -79.31 Example 7 75.69±5.01 71.34±2.92 -49.23 Example 8 48.36±3.23 39.11±2.09 -18.30 The above experimental results show that different preparation methods of Rhodiola rosea root extract and MORUS ALBA leaf extract have a great influence on the whitening and anti-freckle effect of the composition. At the same time, Example 8 also shows that the effect of mulberry branch extract under the formula of the present invention is worse than that of mulberry leaf extract. The experimental results of each group of Examples 3 to 7 also show that the type of fermentation strain used when fermenting mulberry leaves to prepare MORUS ALBA leaf extract has a great influence on the antioxidant effect and whitening effect of the composition. Based on the above experimental results, the best combination of fermentation strains is determined to be Example 6.

[0037] Example 9 In this example, based on the above-mentioned Example 6, the inventors further studied the mass ratio of the root extract of Rhodiola rosea (RHODIOLACRENULATA) and the leaf extract of MORUS ALBA. The evaluation standard was based on the above-mentioned Test Example 1. The experimental results are shown in Table 2: Table 2 Comparison of composition, oxidation effect and whitening effect of control 1 to control 4 Rhodiola rosea root extract Mulberry leaf extract mass percentage DPPH removal rate (%) Tyrosinase inhibitory activity (%) Melanin relative content (%) Comparison 1 2 8 86.05±1.28 85.35±2.68 -60.12 Comparison 2 4 6 89.37±1.97 87.19±1.02 -70.49 Comparison 3 6 4 93.20±2.31 92.15±0.91 -89.92 Comparison 4 8 2 88.31±2.69 90.35±1.72 -83.4 The above experimental results show that the composition provided by the present invention is a combination of Rhodiola rosea (RHODIOLA CRENULATA) root extract and MORUS ALBA (MORUS ALBA) leaf extract, and the mass ratio of the two also has a great influence on the overall antioxidant effect and whitening effect of the composition. When the mass ratio of the two is 6:4, the antioxidant effect and whitening effect of the composition can reach the best.

[0038] Example 10 Hexapeptide-2, also known as whitening peptide, is a small molecule peptide composed of 6 amino acids. It is obtained through artificial synthesis, has a compact molecular structure, and the amino acid sequence contains glycine, glutamic acid, proline, etc. As an antagonist of the α-melanocyte stimulating hormone (α-MSH) receptor, hexapeptide-2 can competitively bind to the receptors on melanocytes, blocking the binding of α-MSH to its receptor, thereby inhibiting melanin synthesis. It can also reduce the activity of related enzymes (such as tyrosinase) in the process of melanin synthesis and reduce the accumulation of melanin in the skin.

[0039] Therefore, based on the above Example 6, this example further explores the combination of the composition and hexapeptide-2: Please refer to Example 6. The raw materials (in parts by mass) used in each composition of the composition provided in this example are as follows: Control 5: 4 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared in the same manner as in Example 6; 4 parts of MORUS ALBA leaf extract, prepared in the same manner as in Example 6; 2 parts of Hexapeptide-2; Control 6: 8 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared by the same method as in Example 6; 8 parts of MORUS ALBA leaf extract, prepared by the same method as in Example 6; Control 7: 4 parts of hexapeptide-2; The above composition was tested for its antioxidant activity and whitening and freckle removal effect. The experimental results are shown in Table 3: Table 3 This example provides the test results of the antioxidant activity and whitening and anti-freckle effect of each composition DPPH removal rate (%) Tyrosinase inhibitory activity (%) Melanin relative content (%) Comparison 5 96.31±1.64 98.31±2.61 -94.19 Comparison 6 90.12±2.30 88.23±3.09 -76.20 Comparison 7 78.01±2.43 95.03±3.21 -89.62 The experiment shows that the addition of hexapeptide-2 can produce a good synergistic effect with the composition provided in the present application (Rhodiola rosea (RHODIOLACRENULATA) root extract and MORUS ALBA (MORUS ALBA) leaf extract).

[0040] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the preferred embodiments, a person skilled in the art should understand that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention. The techniques, shapes, and structural parts not described in detail in the present invention are all well-known technologies.

Claims

1. A whitening and anti-freckle active composition, characterized in that: It includes the following raw materials: Rhodiola rosea root extract, mulberry extract and hexapeptide-2.

2. A whitening and anti-freckle active composition according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 2 to 15 parts of Rhodiola rosea root extract, 1 to 8 parts of MORUS ALBA extract and 1 to 4 parts of hexapeptide-2.

3. A whitening and anti-freckle active composition according to claim 1, characterized in that: The mulberry (MORUS ALBA) extract is any one of a mulberry (MORUS ALBA) leaf extract, a mulberry (MORUS ALBA) branch extract or a mulberry (MORUS ALBA) root bark extract.

4. A method for preparing a whitening and anti-freckle active composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: The root extract of Rhodiola rosea (RHODIOLA CRENULATA) was prepared by water extraction or alcohol extraction; Mulberry (MORUS ALBA) leaf extract was prepared by fermentation method; Wherein, the fermentation method is: S1: Select fresh, pest-free mulberry leaves, dry them to a moisture content of 60% to 70%, and then cut them; S2: Mix the cut mulberry leaves with fermentation liquid at an inoculation ratio of 3% to 10%, wherein the fermentation liquid is 3% to 10% of the mass of the mulberry leaves, and stir and mix thoroughly so that the microorganisms in the fermentation liquid are evenly attached to the mulberry leaves, and ferment at 30°C to 37°C for 3 to 9 days; S3: After the fermentation is completed, the fermented mulberry leaves are decocted and extracted at a solid-liquid ratio of 1:20-50, the extraction time is 1-4 hours, the extraction is performed 2-3 times, and the extracts from multiple extractions are mixed. After mixing, the filtrate is collected by filtering and freeze-drying to obtain a mulberry (MORUS ALBA) leaf extract.

5. The method for preparing a whitening and anti-freckle active composition according to claim 4, characterized in that: The fermented bacterial liquid contains Candida albicans, Bacillus cereus and Aspergillus oryzae, and the ratio of the number of live bacteria of the three bacterial species is 2-3:4-6:1-3.

6. Use of the whitening and anti-freckle active composition according to any one of claims 1 to 3 in skin care products and cosmetics.