A fermentation product and its preparation method and application in the preparation of cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and specifically discloses a fermentation product and a preparation method thereof and an application thereof in the preparation of cosmetics, comprising the following steps: crushing gentian, aloe and sophora flavescens to obtain gentian powder, aloe powder and sophora flavescens powder; adding gentian powder, aloe powder, sophora flavescens powder and glycerol to water, stirring evenly, decocting to obtain a decoction; inoculating plant lactobacillus and adolescent bifidobacterium into the decoction, fermenting, sterilizing to obtain a fermentation liquid; applying a macroporous adsorption resin to the fermentation liquid, eluting at a speed of 1.5BV / h, washing 1.5BV with deionized water, washing 2BV with 80wt% ethanol solution, and collecting the ethanol washing liquid to obtain the fermentation product. The fermentation product has an excellent whitening effect.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a fermentation product, a preparation method thereof, and an application thereof in the preparation of cosmetics. Background Art

[0002] As consumers' demand for whitening products grows, a variety of whitening products have emerged on the market. However, existing whitening products generally have problems such as insignificant whitening effects, easy to cause skin allergies or low safety.

[0003] In the existing technology, whitening skin care products mostly use chemical synthetic ingredients, which may have certain side effects. However, biofermentation technology has gradually become a new trend in the research and development of skin care products due to its natural, safe and efficient characteristics.

[0004] In view of this, this application is filed. Summary of the invention

[0005] The invention provides a fermentation product and a preparation method thereof and application of the fermentation product in the preparation of cosmetics, which have excellent whitening effect.

[0006] The present invention solves the technical problem by adopting the following technical solutions: A method for preparing a fermentation product comprises the following steps: Grinding gentian, aloe and sophora flavescens to obtain gentian powder, aloe powder and sophora flavescens powder; Add gentian powder, aloe powder, sophora flavescens powder and glycerin into water, stir evenly, and boil to obtain a decoction; Inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting, and sterilizing to obtain a fermentation liquid; The macroporous adsorption resin on the fermentation liquid was eluted at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0007] As a preferred embodiment of the present invention, the mass ratio of the gentian powder, aloe powder and sophora flavescens powder is 1:(0.5-2):(1-4).

[0008] As a preferred embodiment of the present invention, the mass ratio of the gentian powder, glycerin and water is 1: (6-10): (15-30).

[0009] As a preferred embodiment of the present invention, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis, and water decoction is (0.005~0.02):(0.005~0.02):1.

[0010] As a preferred embodiment of the present invention, the fermentation temperature is 25-35° C., and the fermentation time is 24-72 hours.

[0011] As a preferred embodiment of the present invention, the macroporous adsorption resin is AB-8.

[0012] The present invention also provides a fermentation product, which is prepared by the above-mentioned preparation method.

[0013] The present invention also provides a whitening composition, comprising the following components in parts by mass: 0.1-0.4 parts of hydrolyzed sclerotium gum, 0.2-0.8 parts of 1,2-hexanediol, 0.2-0.8 parts of acetyl glucosamine, 0.2-1 parts of sodium tocopherol phosphate, 0.6-1.2 parts of hydrolyzed chitosan, 0.8-2 parts of squalane, 1-4 parts of isononyl isononanoate, 2-8 parts of 1,3-propylene glycol, 3-6 parts of fermentation product, 2-10 parts of 1,4-butanediol, and 70-85 parts of water; The fermentation product is the fermentation product described above.

[0014] As a preferred embodiment of the present invention, the composition comprises the following components in parts by mass: 0.1 to 0.3 parts of hydrolyzed sclerotium gum, 0.2 to 0.6 parts of 1,2-hexanediol, 0.2 to 0.6 parts of acetyl glucosamine, 0.2 to 0.8 parts of sodium tocopherol phosphate, 0.6 to 1 parts of hydrolyzed chitin, 0.8 to 1.5 parts of squalane, 1 to 3 parts of isononyl isononanoate, 2 to 6 parts of 1,3-propylene glycol, 4 to 6 parts of fermentation product, 4 to 10 parts of 1,4-butanediol and 75 to 85 parts of water.

[0015] The present invention also provides an application of the whitening composition in the preparation of cosmetics.

[0016] The beneficial effects of the present invention are as follows: (1) The composition of the present invention significantly improves the whitening effect by adding a fermentation product. The fermentation product is of plant origin, mild to the skin, and does not cause allergic reactions. The fermentation product uses gentian, aloe powder, and sophora flavescens as a fermentation base. Gentian can inhibit the expression of TRP-1, TRP-2, and microphthalmia-related transcription factors, thereby inhibiting the production of melanin; aloe powder can effectively inhibit the proliferation of human epidermal melanocytes and has a significant inhibitory effect on intracellular tyrosinase; sophora flavescens can significantly reduce the production of melanin in melanocytes of normal human skin and its transfer to keratinocytes. The three whiten from different whitening mechanisms, thereby exerting a synergistic effect and jointly improving the whitening effect of the fermentation product. DETAILED DESCRIPTION

[0017] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] In the present invention, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0019] In the present invention, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0020] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0021] The reagents or instruments used in the present invention without indicating the manufacturer are all conventional products that can be obtained through commercial purchase.

[0022] The plant lactobacillus of the present invention was purchased from Guangdong Microbiological Culture Collection Center, with the serial number: 1.2865.

[0023] The Bifidobacterium adolescentis of the present invention was purchased from Guangdong Microbiological Culture Collection Center, with the serial number: 1.1262.

[0024] The gentian, aloe powder and sophora flavescens of the present invention were all purchased from Guangzhou Qingping Chinese herbal medicine market.

[0025] The present invention provides a method for preparing a fermentation product, comprising the following steps: Grinding gentian, aloe and sophora flavescens to obtain gentian powder, aloe powder and sophora flavescens powder; Add gentian powder, aloe powder, sophora flavescens powder and glycerin into water, stir evenly, and boil to obtain a decoction; Inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting, and sterilizing to obtain a fermentation liquid; The macroporous adsorption resin on the fermentation liquid was eluted at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0026] The invention obtains a fermentation product with excellent whitening effect by mixed fermentation of gentian, aloe powder and sophora flavescens.

[0027] Among them, gentian, aloe vera and sophora flavescens are all indispensable. When used together as a fermentation base, gentian can inhibit the expression of TRP-1, TRP-2 and microphthalmia-related transcription factors, thereby inhibiting the production of melanin; aloe vera powder can effectively inhibit the proliferation of human epidermal melanocytes and has a significant inhibitory effect on intracellular tyrosinase; sophora flavescens can significantly reduce the production of melanin in melanocytes of normal human skin and its transfer to keratinocytes. The three whiten from different whitening mechanisms, thereby exerting a synergistic effect and jointly improving the whitening effect of the fermentation product.

[0028] Among them, Lactobacillus plantarum and Bifidobacterium adolescentis are introduced into the system together, and the whitening effect is further improved under the fermentation of probiotics.

[0029] Among them, the macroporous adsorption resin on the fermentation liquid is further enriched with active ingredients with whitening effect, further improving the whitening effect.

[0030] In one embodiment, the mass ratio of the gentian powder, aloe powder and sophora flavescens powder is 1:(0.5-2):(1-4).

[0031] In one embodiment, the mass ratio of the gentian powder, glycerin and water is 1: (6-10): (15-30).

[0032] In one embodiment, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis, and water decoction is (0.005~0.02):(0.005~0.02):1.

[0033] In one embodiment, the fermentation temperature is 25-35° C., and the fermentation time is 24-72 hours.

[0034] In one embodiment, the macroporous adsorption resin is AB-8.

[0035] The present invention also provides a fermentation product, which is prepared by the above-mentioned preparation method.

[0036] The present invention also provides a whitening composition, comprising the following components in parts by mass: 0.1-0.4 parts of hydrolyzed sclerotium gum, 0.2-0.8 parts of 1,2-hexanediol, 0.2-0.8 parts of acetyl glucosamine, 0.2-1 parts of sodium tocopherol phosphate, 0.6-1.2 parts of hydrolyzed chitosan, 0.8-2 parts of squalane, 1-4 parts of isononyl isononanoate, 2-8 parts of 1,3-propylene glycol, 3-6 parts of fermentation product, 2-10 parts of 1,4-butanediol, and 70-85 parts of water; The fermentation product is the fermentation product described above.

[0037] In one embodiment, the invention comprises the following components in parts by mass: 0.1 to 0.3 parts of hydrolyzed sclerotium gum, 0.2 to 0.6 parts of 1,2-hexanediol, 0.2 to 0.6 parts of acetyl glucosamine, 0.2 to 0.8 parts of sodium tocopherol phosphate, 0.6 to 1 parts of hydrolyzed chitin, 0.8 to 1.5 parts of squalane, 1 to 3 parts of isononyl isononanoate, 2 to 6 parts of 1,3-propylene glycol, 4 to 6 parts of fermentation product, 4 to 10 parts of 1,4-butanediol, and 75 to 85 parts of water.

[0038] In one embodiment, the composition further comprises an auxiliary material acceptable in the cosmetic field.

[0039] In one embodiment, the auxiliary material includes at least one of a solvent, a thickener, a moisturizer, an emollient, an emulsifier, a preservative, an antioxidant, a pH adjuster, a penetration enhancer, a liposome, a chelating agent, a surfactant, a flavor, and a pigment.

[0040] It should be noted that those skilled in the art can select the type of auxiliary materials according to actual needs, as long as the corresponding effects are achieved.

[0041] Exemplarily, the composition may include a humectant, and suitable humectants include, but are not limited to, at least one of dipropylene glycol, polyethylene glycol, xylitol, diglycerol, maltitol, maltose, D-mannitol, pentylene glycol, dipropylene glycol, polypropylene glycol, ethylene glycol, neopentyl glycol, erythritol, sorbitol, mannitol, xylitol, glucose, lactose, mannose, maltose, fructose, inositol, sorbitol, sorbitan, trehalose, and starch hydrolyzate.

[0042] Exemplarily, the composition may include a thickener, and suitable thickeners include, but are not limited to, at least one of carbomer, hydroxyethyl cellulose, xanthan gum, sodium polyacrylate, acrylic acid (ester) cross-linked polymer, and xanthan gum.

[0043] Exemplarily, the composition may include an emollient, suitable emollients include, but are not limited to, caprylic / capric triglyceride, isohexadecane, squalane, polydimethylsiloxane, cyclopentasiloxane, diisostearyl malate, dicetearyl dimer dimer linoleate, hexyl laurate, polyglyceryl 2-diisostearate, hydrogenated polyisobutene, pentaerythritol, tetraisostearate, caprylic / capric triglyceride, octyldodecanol, dicaprylyl carbonate, macadamia oil, shea butter, jojoba oil, lanolin, white oil, almond oil, olive oil, jojoba oil, castor oil, C12-15 alkyl benzoate, isononyl isononanoate, dioctyl adipate, octyl stearate, hexyl laurate, coco-caprylate, and caprate.

[0044] Exemplarily, the composition may include an emulsifier, suitable emulsifiers include, but are not limited to, at least one of polyglyceryl-3 diisostearate, polyglyceryl-10 stearate, polyglyceryl-10 laurate, polyglyceryl-10 myristate, polyglyceryl-5 trioleate, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, PEG-30 soy sterol, PPG-13-decyltetradecyl alcohol polyether-24, nonylphenol polyether-12, PEG-35 castor oil / PEG-50 hydrogenated castor oil / nonylphenol polyether-12 / propylene glycol, steareth-2, steareth-21, and hydrogenated lecithin.

[0045] Exemplarily, the composition may include a surfactant, and suitable surfactants include, but are not limited to, lauryl glucoside, sodium cocoyl glutamate, sodium lauryl sulfate, sodium laureth sulfate, sodium dodecylbenzene sulfonate, cocamidopropyl betaine, sodium lauryl glucose formate, and combinations thereof.

[0046] Exemplarily, the composition may include a pH adjuster. Suitable pH adjusters include, but are not limited to, arginine, triethanolamine, citric acid, sodium citrate, and combinations thereof.

[0047] Exemplarily, the composition may include a colorant, and suitable colorants include, but are not limited to, white, black, yellow, blue, green, pink, red, orange, purple, indigo, brown, and the like, and combinations thereof.

[0048] The present invention also provides an application of the whitening composition in the preparation of cosmetics.

[0049] In one embodiment, the cosmetic is in the form of an aqueous solution, emulsion, cream, gel, mask or essence.

[0050] The present invention is further described below with reference to specific embodiments.

[0051] Example 1 A whitening composition comprises the following components in parts by mass: 0.2 parts of hydrolyzed sclerotium gum, 0.5 parts of 1,2-hexanediol, 0.5 parts of acetyl glucosamine, 0.5 parts of sodium tocopherol phosphate, 0.8 parts of hydrolyzed chitin, 1 part of squalane, 2 parts of isononyl isononanoate, 4 parts of 1,3-propylene glycol, 5 parts of fermentation product, 6 parts of 1,4-butanediol, and 79.5 parts of water.

[0052] The method for preparing the fermentation product comprises the following steps: (1) Grinding gentian, aloe vera and sophora flavescens into 100 meshes respectively to obtain gentian powder, aloe vera powder and sophora flavescens powder respectively; (2) adding 1 part by weight of gentian powder, 1 part by weight of aloe powder, 2 parts by weight of sophora flavescens powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting at 30° C. for 48 h, and sterilizing to obtain a fermentation liquid; Wherein, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis and water decoction is 0.01:0.01:1.

[0053] (4) The fermentation liquid was eluted with AB-8 macroporous adsorption resin at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0054] Example 2 A whitening composition comprises the following components in parts by mass: 0.2 parts of hydrolyzed sclerotium gum, 0.2 parts of 1,2-hexanediol, 0.8 parts of acetyl glucosamine, 0.2 parts of sodium tocopherol phosphate, 1.2 parts of hydrolyzed chitin, 0.8 parts of squalane, 4 parts of isononyl isononanoate, 2 parts of 1,3-propylene glycol, 3.5 parts of fermentation product, 10 parts of 1,4-butanediol, and 77.1 parts of water.

[0055] The method for preparing the fermentation product comprises the following steps: (1) Grinding gentian, aloe vera and sophora flavescens into 100 meshes respectively to obtain gentian powder, aloe vera powder and sophora flavescens powder respectively; (2) adding 1 part by weight of gentian powder, 2 parts by weight of aloe powder, 1 part by weight of sophora flavescens powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting at 30° C. for 48 h, and sterilizing to obtain a fermentation liquid; Wherein, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis and water decoction is 0.015:0.015:1.

[0056] (4) The fermentation liquid was eluted with AB-8 macroporous adsorption resin at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0057] Example 3 A whitening composition comprises the following components in parts by mass: 0.2 parts of hydrolyzed sclerotium gum, 0.8 parts of 1,2-hexanediol, 0.2 parts of acetyl glucosamine, 1 part of sodium tocopherol phosphate, 0.6 parts of hydrolyzed chitosan, 2 parts of squalane, 1 part of isononyl isononanoate, 8 parts of 1,3-propylene glycol, 4 parts of fermentation product, 5 parts of 1,4-butanediol and 77.2 parts of water.

[0058] The method for preparing the fermentation product comprises the following steps: (1) Grinding gentian, aloe vera and sophora flavescens into 100 meshes respectively to obtain gentian powder, aloe vera powder and sophora flavescens powder respectively; (2) adding 1 part by weight of gentian powder, 1 part by weight of aloe powder, 3 parts by weight of sophora flavescens powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting at 30° C. for 48 h, and sterilizing to obtain a fermentation liquid; Wherein, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis and water decoction is 0.02:0.005:1.

[0059] (4) The fermentation liquid was eluted with AB-8 macroporous adsorption resin at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0060] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not contain fermentation products, and the other aspects are the same.

[0061] A whitening composition comprises the following components in parts by mass: 0.2 parts of hydrolyzed sclerotium gum, 0.5 parts of 1,2-hexanediol, 0.5 parts of acetyl glucosamine, 0.5 parts of sodium tocopherol phosphate, 0.8 parts of hydrolyzed chitosan, 1 part of squalane, 2 parts of isononyl isononanoate, 4 parts of 1,3-propylene glycol, 6 parts of 1,4-butylene glycol, and 84.5 parts of water.

[0062] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that no gentian powder is added in the preparation method of the fermentation product of Comparative Example 2 (the total amount of gentian powder, aloe powder and sophora flavescens powder remains unchanged), and the others are the same.

[0063] The method for preparing the fermentation product comprises the following steps: (1) Grinding aloe vera and sophora flavescens into 100 meshes respectively to obtain aloe vera powder and sophora flavescens powder respectively; (2) adding 1.5 parts by weight of aloe vera powder, 2.5 parts by weight of Sophora flavescens powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting at 30° C. for 48 h, and sterilizing to obtain a fermentation liquid; Wherein, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis and water decoction is 0.01:0.01:1.

[0064] (4) The fermentation liquid was eluted with AB-8 macroporous adsorption resin at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0065] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that no aloe powder is added in the preparation method of the fermentation product of Comparative Example 3 (the total amount of gentian powder, aloe powder and sophora flavescens powder remains unchanged), and the others are the same.

[0066] The method for preparing the fermentation product comprises the following steps: (1) Grind gentian and sophora flavescens into 100 meshes to obtain gentian powder and sophora flavescens powder respectively; (2) adding 1.5 parts by weight of gentian powder, 2.5 parts by weight of sophora flavescens powder, and 6 parts by weight of glycerol to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting at 30° C. for 48 h, and sterilizing to obtain a fermentation liquid; Wherein, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis and water decoction is 0.01:0.01:1.

[0067] (4) The fermentation liquid was eluted with AB-8 macroporous adsorption resin at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0068] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that in the preparation method of the fermentation product of Comparative Example 4, no Sophora flavescens powder is added (the total amount of gentian powder, aloe powder, and Sophora flavescens powder remains unchanged), and the others are the same.

[0069] The method for preparing the fermentation product comprises the following steps: (1) Grinding gentian and aloe vera into 100 meshes to obtain gentian powder and aloe vera powder respectively; (2) adding 2 parts by weight of gentian powder, 2 parts by weight of aloe powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting at 30° C. for 48 h, sterilizing, and obtaining a fermentation liquid; Wherein, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis and water decoction is 0.01:0.01:1.

[0070] (4) The fermentation liquid was eluted with AB-8 macroporous adsorption resin at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0071] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that only Lactobacillus plantarum was inoculated in Comparative Example 5 (the total inoculation amount of fungi remained unchanged), and the other contents were the same.

[0072] The method for preparing the fermentation product comprises the following steps: (1) Grinding gentian, aloe vera and sophora flavescens into 100 meshes respectively to obtain gentian powder, aloe vera powder and sophora flavescens powder respectively; (2) adding 1 part by weight of gentian powder, 1 part by weight of aloe powder, 2 parts by weight of sophora flavescens powder, and 6 parts by weight of glycerol to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) inoculating Lactobacillus plantarum into the water decoction, fermenting at 30° C. for 48 h, sterilizing, and obtaining a fermentation liquid; Wherein, the mass ratio of the plant lactobacillus and the water decoction is 0.02:1.

[0073] (4) The fermentation liquid was eluted with AB-8 macroporous adsorption resin at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0074] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 is not treated with a macroporous adsorption resin, and the other aspects are the same.

[0075] The method for preparing the fermentation product comprises the following steps: (1) Grinding gentian, aloe vera and sophora flavescens into 100 meshes respectively to obtain gentian powder, aloe vera powder and sophora flavescens powder respectively; (2) adding 1 part by weight of gentian powder, 1 part by weight of aloe powder, 2 parts by weight of sophora flavescens powder, and 6 parts by weight of glycerol to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting at 30° C. for 48 h, sterilizing, and obtaining a fermentation product; Wherein, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis and water decoction is 0.01:0.01:1.

[0076] Comparative Example 7 The difference between Comparative Example 7 and Example 1 is that in the preparation method of the fermentation product of Comparative Example 7, an equal amount of Morus alba bark is used to replace Sophora flavescens, and the rest are the same.

[0077] The method for preparing the fermentation product comprises the following steps: (1) Grinding gentian, aloe vera and mulberry bark into 100 meshes respectively to obtain gentian powder, aloe vera powder and mulberry bark powder respectively; (2) adding 1 part by weight of gentian powder, 1 part by weight of aloe powder, 2 parts by weight of mulberry bark powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting at 30° C. for 48 h, sterilizing, and obtaining a fermentation liquid; Wherein, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis and water decoction is 0.01:0.01:1.

[0078] (4) The fermentation liquid was eluted with AB-8 macroporous adsorption resin at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0079] Comparative Example 8 The difference between Comparative Example 8 and Example 1 is that in the preparation method of the fermentation product of Comparative Example 8, an equal amount of coix seed is used to replace gentian, and the other parts are the same.

[0080] The method for preparing the fermentation product comprises the following steps: (1) Grinding coix seed, aloe vera and sophora flavescens into 100 meshes respectively to obtain coix seed powder, aloe vera powder and sophora flavescens powder respectively; (2) adding 1 part by weight of coix seed powder, 1 part by weight of aloe powder, 2 parts by weight of sophora flavescens powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting at 30° C. for 48 h, sterilizing, and obtaining a fermentation liquid; Wherein, the mass ratio of Lactobacillus plantarum, Bifidobacterium adolescentis and water decoction is 0.01:0.01:1.

[0081] (4) The fermentation liquid was eluted with AB-8 macroporous adsorption resin at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

[0082] Test Case 1. Whitening efficacy test (conventional experiment): 110 volunteers were selected and randomly divided into 11 groups of 10 people in each group. The volunteers in each group tried the compositions described in Examples 1 to 3 and Comparative Examples 1 to 8 respectively.

[0083] The criteria for inclusion of volunteers are: aged 20 to 30 years old, female; those who have not used hormone drugs and immunosuppressants in the past month and can cooperate well with the experimenters; those who can read and understand all the contents of the informed consent form and voluntarily sign the informed consent form; and those who agree not to use any cosmetics, drugs, and health products that may affect the results during the trial.

[0084] Exclusion criteria: People with highly allergic constitution; people with a history of allergic reaction to cosmetics; women who are pregnant, breastfeeding or planning to become pregnant during the test period; people with severe heart, liver, and kidney damage and severe immunodeficiency; people with mental illness, severe endocrine diseases, and oral contraceptives; people who have participated in drug clinical trials or other trials within 30 days, or have systemically used drugs that may affect the test results within the past week; people who have taken oral or external beauty products that may affect the test results within 2 weeks; people who are considered by the researchers to be unsuitable for participating in this test.

[0085] Suspension and exit criteria: Failure to apply the test sample according to the test requirements or failure to make follow-up records as required during the trial; serious illness or serious adverse reactions during use.

[0086] 2. Test method: Volunteers applied the composition to their entire face after cleansing in the morning and evening every day for 28 consecutive days.

[0087] On the 0th, 7th and 28th days of the trial, the melanin index (MI) of the volunteers was tested using the skin red-melanin tester produced by the German company Courage+Khazaka. The average value of three tests was taken each time, and the melanin change rate [(MIn-MI0) / (MI0)] was calculated. The average value of the melanin change rate of each group of volunteers was taken. The test results are shown in Table 1.

[0088] Table 1

[0089] It can be seen from Table 1 that the composition of the present invention has an excellent whitening effect.

[0090] Comparing Examples 1 to 3, it can be seen that Example 1 is the best embodiment of the present invention and has the best whitening effect.

[0091] By comparing Example 1 with Comparative Example 1, it can be seen that the fermentation product of the present invention has a very significant improvement in the whitening effect.

[0092] By comparing Example 1 with Comparative Examples 2-4, 7 and 8, it can be seen that the gentian, aloe powder and sophora flavescens described in the present invention are all indispensable in the preparation of the fermentation product. The combination of the three has an excellent synergistic effect. When any one of them is missing, the whitening effect will be significantly reduced.

[0093] By comparing Example 1 with Comparative Example 5, it can be seen that the present invention requires the simultaneous addition of Lactobacillus plantarum and Bifidobacterium adolescentis, and the fermentation product of the two has a better whitening effect.

[0094] By comparing Example 1 with Comparative Example 6, it can be seen that the present invention further improves the whitening effect by performing macroporous adsorption resin treatment.

[0095] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing a fermentation product, characterized in that: The following steps are involved: Grinding gentian, aloe and sophora flavescens to obtain gentian powder, aloe powder and sophora flavescens powder; Add gentian powder, aloe powder, sophora flavescens powder and glycerin into water, stir evenly, and boil to obtain a decoction; Inoculating Lactobacillus plantarum and Bifidobacterium adolescentis into the water decoction, fermenting, and sterilizing to obtain a fermentation liquid; The macroporous adsorption resin on the fermentation liquid was eluted at a rate of 1.5 BV / h, 1.5 BV was washed with deionized water, 2 BV was washed with 80 wt% ethanol solution, and the ethanol washing liquid was collected, which was the fermentation product.

2. The method for preparing a fermentation product according to claim 1, characterized in that: The mass ratio of the gentian powder, aloe powder and sophora flavescens powder is 1:(0.5-2):(1-4).

3. The method for preparing a fermentation product according to claim 1, characterized in that: The mass ratio of the gentian powder, glycerin and water is 1: (6-10): (15-30).

4. The method for preparing a fermentation product according to claim 1, characterized in that: The mass ratio of the plant lactobacillus, the adolescent bifidobacterium and the water decoction is (0.005-0.02): (0.005-0.02):

1.

5. The method for preparing a fermentation product according to claim 1, characterized in that: The fermentation temperature is 25-35° C., and the fermentation time is 24-72 hours.

6. The method for preparing a fermentation product according to claim 1, characterized in that: The macroporous adsorption resin is AB-8.

7. A fermentation product, characterized in that The method is prepared by any one of claims 1 to 6.

8. A whitening composition, characterized in that: The invention comprises the following components in parts by weight: 0.1-0.4 parts of hydrolyzed sclerotium gum, 0.2-0.8 parts of 1,2-hexanediol, 0.2-0.8 parts of acetyl glucosamine, 0.2-1 parts of sodium tocopherol phosphate, 0.6-1.2 parts of hydrolyzed chitosan, 0.8-2 parts of squalane, 1-4 parts of isononyl isononanoate, 2-8 parts of 1,3-propylene glycol, 3-6 parts of fermentation product, 2-10 parts of 1,4-butylene glycol, and 70-85 parts of water; The fermentation product is the fermentation product according to claim 7.

9. The whitening composition according to claim 1, characterized in that The invention comprises the following components in parts by mass: 0.1-0.3 parts of hydrolyzed sclerotium gum, 0.2-0.6 parts of 1,2-hexanediol, 0.2-0.6 parts of acetyl glucosamine, 0.2-0.8 parts of sodium tocopherol phosphate, 0.6-1 parts of hydrolyzed chitosan, 0.8-1.5 parts of squalane, 1-3 parts of isononyl isononanoate, 2-6 parts of 1,3-propylene glycol, 4-6 parts of fermentation product, 4-10 parts of 1,4-butylene glycol and 75-85 parts of water.

10. Use of the whitening composition according to any one of claims 8 to 9 in the preparation of cosmetics.

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