A traditional Chinese medicine active composition, shampoo and preparation method

Abstract

The invention discloses a Chinese medicine active composition, shampoo and preparation method, which relates to the technical field of cosmetics, and comprises the following components: 8-10% of Platycladus orientalis leaves; 1-3% of Polygonum multiflorum; 1-3% of Ginseng; 1-3% of Sophora flavescens; 1-3% of Chuanxiong; and 78-88% of deionized water. The technical scheme of the invention uses Platycladus orientalis leaves, Polygonum multiflorum, Ginseng, Sophora flavescens and Chuanxiong as raw materials, and the raw materials are mutually matched, and the composition prepared by the combination of the raw materials has the function of inhibiting oil production and significantly improving the anti-hair loss efficiency; the Chinese medicine composition can regulate the level of male hormones (such as DHT), reduce its binding with hair follicle receptors, thereby inhibiting hair follicle atrophy; the Chinese medicine composition can also inhibit the synthesis of male hormones, block the generation of DHT, and protect hair follicles from damage; the Chinese medicine composition contains rich antioxidants, protects hair follicles from oxidative damage, and promotes hair growth; the Chinese medicine composition and shampoo can nourish hair roots, improve hair quality, and enhance the elasticity and gloss of hair by providing necessary nutrition and nutrients.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a traditional Chinese medicine active composition, a shampoo and a preparation method thereof. Background Art

[0002] With the accelerated pace of life, increased work pressure and the influence of bad living habits, more and more people are facing the troubles of greasy hair and hair loss. In the pursuit of a healthy life, consumers' requirements for shampoo are no longer limited to the cleaning function, but pay more attention to its conditioning of scalp health and improvement of hair problems.

[0003] Most shampoos on the market contain chemical extracts, which may cause harm to human health. Although Chinese herbal shampoos are advertised to have effects such as preventing hair loss and controlling oil, most products do not contain enough Chinese herbal ingredients and are not very effective.

[0004] The scalp is a key factor in hair growth. Oily scalp and the resulting seborrheic alopecia are common problems. Seborrheic alopecia is characterized by excessive scalp oil, increased dandruff, greasy itching and other symptoms.

[0005] Disadvantages of existing technology

[0006] (1) Deficiencies of existing products: The oil control and anti-hair loss products currently on the market, especially those for external use, are technically difficult and have unsatisfactory effects.

[0007] (2) Disadvantages of Chinese herbal shampoos: Most Chinese herbal shampoos only contain a small amount of Chinese herbal extracts, and the content of effective Chinese herbal ingredients is insufficient, failing to fully exert the anti-hair loss and oil control effects of Chinese herbal medicine.

[0008] (3) Poor anti-dandruff effect: Existing anti-dandruff shampoos have limited effect on oily hair and dandruff problems. Summary of the invention

[0009] The technical solution of the present invention to solve the above technical problems is to provide a traditional Chinese medicine active composition, which comprises the following components in percentage by weight:

[0010]

[0011]

[0012] In order to solve the above technical problems, the present invention provides an oil-control and anti-hair loss shampoo containing a traditional Chinese medicine active composition, comprising phase A, phase B, phase C, phase D and phase F;

[0013] The A phase is divided into an A1 phase and an A2 phase, and the A1 phase includes the following components in percentage by weight:

[0014]

[0015] The A2 phase includes the following components, and the component mass percentages are:

[0016] Citric acid 0.05-0.1%;

[0017] Disodium EDTA 0.05-0.15%;

[0018] The phase B includes the following components, and the component mass percentages are:

[0019] Ammonium laureth sulfate 10-20%;

[0020] Ammonium lauryl sulfate 4-6%;

[0021] The C phase raw material includes the following components, and the component mass percentages are:

[0022] Cetyl alcohol 0.2-0.8%;

[0023] Cocamide MEA 0.5-1.5%;

[0024] Ethylene glycol distearate 1-2%;

[0025] The D phase is divided into a D1 phase and a D2 phase, and the D1 phase includes the following components in percentage by mass:

[0026] Cocamidopropyl Betaine 2-4%;

[0027] Piroctone olamine salt 0.2-0.5%;

[0028] The D2 phase includes the following components, and the component mass percentages are:

[0029] Dimethicone, water, cocamidopropyl betaine, guar hydroxypropyltrimonium chloride, C12-15 pareth-3, phenoxyethanol 1-3%;

[0030] The E phase includes the following components, and the component mass percentages are:

[0031]

[0032]

[0033] The F phase includes the following components, and the component mass percentages are:

[0034] Sodium citrate 0.02-0.08%;

[0035] Sodium chloride 0.2-0.8%;

[0036] In order to solve the above technical problems, the present invention also provides a method for preparing an oil-controlling and anti-hair loss shampoo, which is used to prepare the oil-controlling and anti-hair loss shampoo containing the Chinese medicine active composition as described above, comprising the following steps:

[0037] Step 1: Add the A1 phase water into the main pot, add the remaining ingredients of the A1 phase under homogenization, disperse completely, add the A2 phase ingredients, and stir thoroughly;

[0038] Step 2: Add phase B, heat to 80-85°C with stirring, and stir thoroughly until completely dissolved;

[0039] Step 3: Add phase C and stir until it is completely dissolved, and keep the temperature constant for 20 minutes;

[0040] Step 4: Add the raw materials of phase D1, stir until completely dissolved, and start stirring to cool down;

[0041] Step 5: Cool down to 70-75°C, add D2 phase, stir evenly, and continue to cool down;

[0042] Step 6: Cool down to 45°C, add the raw materials of phase E in sequence, and stir evenly;

[0043] Step 7: Add the raw materials of phase F in sequence, stir and dissolve completely to obtain the oil-control and anti-hair loss shampoo.

[0044] Further, in step 6, the preparation method of the active Chinese medicine composition in phase E is:

[0045] Weigh Platycladus orientalis leaves, Polygonum multiflorum, Ginseng, Sophora flavescens and Ligusticum chuanxiong, and crush them into coarse powder;

[0046] Add water, treat with ultrasound for 30 min, and filter through gauze;

[0047] The mixture was centrifuged at 12000 r / min, filtered through a 300-mesh sieve, and fixed to volume to obtain a traditional Chinese medicine active composition.

[0048] Compared with the prior art, the present invention has the following beneficial effects:

[0049] 1. The present invention uses Platycladus orientalis leaves, Polygonum multiflorum, ginseng, Sophora flavescens, and Ligusticum chuanxiong as raw materials, and the raw materials are combined with each other. The composition prepared by the combination of the raw materials has the function of inhibiting oil production, significantly improving the anti-hair loss efficiency, and improving the use efficiency of the product.

[0050] 2. The Chinese medicine composition of the present invention can regulate the level of male hormones (such as DHT), reduce their binding to hair follicle receptors, and thus inhibit hair follicle atrophy, which is one of the important mechanisms for preventing hair loss. The Chinese medicine composition of the present invention can also inhibit the synthesis of male hormones, block the production of DHT, and protect hair follicles from damage.

[0051] 3. The Chinese medicine composition and shampoo of the present invention contain rich antioxidants, such as vitamins C and E, which can remove free radicals, protect hair follicles from oxidative damage, and promote hair growth; they can also reduce scalp inflammation, improve the hair follicle microenvironment, and are beneficial to hair growth.

[0052] 4. The Chinese medicine composition and shampoo of the present invention have anti-inflammatory effects, can inhibit the release of scalp inflammatory factors, reduce inflammatory reactions, and protect hair follicles; they can also improve scalp microcirculation and promote hair growth.

[0053] 5. The Chinese medicinal composition and shampoo of the present invention can nourish hair roots, improve hair quality, and enhance the elasticity and luster of hair by providing necessary nutrition and nutrients. BRIEF DESCRIPTION OF THE DRAWINGS

[0054] Figure 1 This is a graph showing the hair growth on the back of mice in the efficacy evaluation of the anti-hair loss experiment in Example 2 and Test Example 1 of the present invention;

[0055] Figure 2 The length of new hair on the back of mice was evaluated for the efficacy of the anti-hair loss experiment in Example 2 of the present invention and Test Example 1 (Note: #p<0.05, ##p<0.01, ###p<0.001 compared with the blank group; *p<0.05, **p<0.01, ***p<0.001 compared with the model group);

[0056] Figure 3 The weight of new hair on the back of mice was evaluated for the anti-hair loss experimental efficacy of Example 2 and Test Example 1 of the present invention (Note: #p<0.05, ##p<0.01, ###p<0.001 compared with the blank group; *p<0.05, **p<0.01, ***p<0.001 compared with the model group);

[0057] Figure 4 This is a diagram showing the results of lipid droplet oil red staining for the oil control experiment efficacy evaluation of Example 2 and Test Example 2 of the present invention;

[0058] Figure 5 This is a differential analysis diagram of lipid droplet synthesis in the oil control experiment efficacy evaluation of Example 2 of the present invention and Test Example 2;

[0059] Figure 6 This is a diagram showing the results of lipid droplet oil red staining for the oil control experiment efficacy evaluation of Comparative Example 2 and Test Example 2 of the present invention;

[0060] Figure 7 This is a differential analysis diagram of lipid droplet synthesis in the oil control experiment efficacy evaluation of Comparative Example 2 and Test Example 2 of the present invention. DETAILED DESCRIPTION

[0061] The invention provides a traditional Chinese medicine active composition, a shampoo and a preparation method, aiming to achieve the effects of oil control and hair loss prevention through the active ingredients of the traditional Chinese medicine.

[0062] The following will describe the active Chinese medicinal composition with anti-hair loss effect proposed by the present invention in specific embodiments:

[0063] In the technical solution of this embodiment, a Chinese medicine active composition is provided, wherein the Chinese medicine active composition comprises the following components in the following mass percentages:

[0064]

[0065] An oil-control and anti-hair loss shampoo containing a traditional Chinese medicine active composition, comprising phase A, phase B, phase C, phase D and phase F;

[0066] The A phase is divided into an A1 phase and an A2 phase, and the A1 phase includes the following components in percentage by weight:

[0067]

[0068] The A2 phase includes the following components, and the component mass percentages are:

[0069] Citric acid 0.05-0.1%;

[0070] Disodium EDTA 0.05-0.15%;

[0071] The phase B includes the following components, and the component mass percentages are:

[0072] Ammonium laureth sulfate 10-20%;

[0073] Ammonium lauryl sulfate 4-6%;

[0074] The C phase raw material includes the following components, and the component mass percentages are:

[0075] Cetyl alcohol 0.2-0.8%;

[0076] Cocamide MEA 0.5-1.5%;

[0077] Ethylene glycol distearate 1-2%;

[0078] The D phase is divided into a D1 phase and a D2 phase, and the D1 phase includes the following components in percentage by mass:

[0079] Cocamidopropyl Betaine 2-4%;

[0080] Piroctone olamine salt 0.2-0.5%;

[0081] The D2 phase includes the following components, and the component mass percentages are:

[0082] Dimethicone, water, cocamidopropyl betaine, guar hydroxypropyltrimonium chloride, C12-15 pareth-3, phenoxyethanol 1-3%;

[0083] The E phase includes the following components, and the component mass percentages are:

[0084]

[0085] The F phase includes the following components, and the component mass percentages are:

[0086] Sodium citrate 0.02-0.08%;

[0087] Sodium chloride 0.2-0.8%;

[0088] A method for preparing an oil-controlling and anti-hair loss shampoo, which is used to prepare the oil-controlling and anti-hair loss shampoo containing the traditional Chinese medicine active composition as described above, comprising the following steps:

[0089] Step 1: Add the A1 phase water into the main pot, add the remaining ingredients of the A1 phase under homogenization, disperse completely, add the A2 phase ingredients, and stir thoroughly;

[0090] Step 2: Add phase B, heat to 80-85°C with stirring, and stir thoroughly until completely dissolved;

[0091] Step 3: Add phase C and stir until it is completely dissolved, and keep the temperature constant for 20 minutes;

[0092] Step 4: Add the raw materials of phase D1, stir until completely dissolved, and start stirring to cool down;

[0093] Step 5: Cool down to 70-75°C, add D2 phase, stir evenly, and continue to cool down;

[0094] Step 6: Cool down to 45°C, add the raw materials of phase E in sequence, and stir evenly;

[0095] Step 7: Add the raw materials of phase F in sequence, stir and dissolve completely to obtain the oil-control and anti-hair loss shampoo.

[0096] Further, in step 6, the preparation method of the active Chinese medicine composition in phase E is:

[0097] Weigh Platycladus orientalis leaves, Polygonum multiflorum, Ginseng, Sophora flavescens and Ligusticum chuanxiong, and crush them into coarse powder;

[0098] Add water, treat with ultrasound for 30 min, and filter through gauze;

[0099] The mixture was centrifuged at 12000 r / min, filtered through a 300-mesh sieve, and fixed to volume to obtain a traditional Chinese medicine active composition.

[0100] Platycladus orientalis is widely used in traditional Chinese medicine to treat hair loss. It is believed to cool the blood and stop bleeding, clear away heat and detoxify, effectively inhibit scalp inflammation, and promote healthy hair follicles. Especially for hair loss problems caused by excessive oil secretion or clogged hair follicles, Platycladus orientalis shampoo can help improve the scalp environment and promote hair growth, thereby reducing hair loss. Platycladus orientalis also has the effect of regulating the secretion of oil on the scalp. It can help control the excessive oil secretion of the scalp, thereby reducing greasiness and dandruff. This is a very useful feature for people who often feel scalp discomfort due to excessive oil secretion or need to wash their hair frequently. As a natural ingredient, Platycladus orientalis is relatively safe to use and is suitable for most people.

[0101] As a traditional Chinese medicine, He Shou Wu has the effect of nourishing and strengthening hair, helping to improve the hair growth environment and promote hair growth. He Shou Wu shampoo contains rich nutrients, which can effectively nourish the scalp and promote the health of hair follicles, thereby reducing hair loss problems. He Shou Wu shampoo has excellent cleaning effects, can effectively remove dandruff, control oil secretion, keep the scalp fresh, and reduce scalp itching. The oil control ingredients can effectively regulate the oil secretion of the scalp, reduce the greasy feeling of the hair, and keep the hair fresh.

[0102] Ginseng contains ingredients such as ginsenosides, which can promote blood circulation in the scalp and increase the nutrients needed by the hair, thereby contributing to the healthy development of hair follicles and hair growth. Experiments have shown that ginsenosides can effectively promote blood circulation in the scalp, increase the nutrients needed by the hair, repair the hair, and strengthen the hair. It is a panacea for preventing hair loss and fixing hair. Ginseng has a powerful bactericidal and anti-inflammatory effect, which can balance the scalp environment and balance the oil secretion. This keeps the scalp clean, so that the new hair can grow and stay, reducing the problem of hair loss caused by excessive oil secretion. The various nourishing ingredients in ginseng can improve the toughness of hair and reduce hair loss and hair breakage. Ginsenosides have been proven through experiments to effectively promote blood circulation, fill in and increase the missing nutrients of the hair, repair damaged hair follicles, strengthen the hair roots and increase the toughness of the hair, and have a good effect on fixing hair and preventing hair loss. Ginseng shampoo uses a silicone-free formula, which is gentler and does not irritate the scalp, and safely activates hair follicles. In addition, natural extracts added to the product, such as Chuanxiong extract, Platycladus orientalis leaf extract and panthenol, can further improve the scalp environment and make the hair healthier and stronger.

[0103] Sophora flavescens has the effects of clearing away dampness and heat, dispelling wind and relieving itching. It can balance oil secretion, dredge and astringe pores, and remove toxins and impurities in the scalp. This is very helpful for preventing and treating seborrheic alopecia, because excessive oil and scalp dirt can clog hair follicles and affect the normal growth of hair. Sophora flavescens also shows anti-inflammatory and anti-androgenic effects, which can effectively improve scalp itching, restore a healthy scalp, and control oil without dryness. This is particularly effective for hair loss problems caused by changes in hormone levels. Through its pharmacological effects, Sophora flavescens can dilate the capillaries in the head, promote blood circulation in the head, and increase the nutrient supply of the hair. This helps to improve the problem of fragile and easily broken hair roots, achieve the perfect experience of strong hair roots, and reproduce thick hair.

[0104] Chuanxiong contains volatile oils, alkaloids, phenolic components, lipids, ferulic acid, and essential trace elements such as zinc, copper, and iron. These ingredients can dilate the capillaries in the head, promote blood circulation in the head, increase the nutritional supply of the hair, prevent the hair from becoming brittle, prevent the hair from drying and falling, and keep the hair oily and shiny. This product does not add silicone oil, so shampooing is burden-free, and the hair is refreshed and moisturized without dryness, making the scalp and hair follicles healthier. Chuanxiong anti-hair loss and oil control shampoo is suitable for people with itchy scalp and dandruff, which increases hair nutrition and makes the hair smooth and shiny. At the same time, it is also suitable for people with hair loss and oily hair, effectively preventing hair loss and making people feel fresh and natural.

[0105] Compared with the prior art, the present invention has the following beneficial effects:

[0106] 1. The present invention uses Platycladus orientalis leaves, Polygonum multiflorum, ginseng, Sophora flavescens, and Ligusticum chuanxiong as raw materials, and the raw materials are combined with each other. The composition prepared by the combination of the raw materials has the function of inhibiting oil production, significantly improving the anti-hair loss efficiency, and improving the use efficiency of the product.

[0107] 2. The Chinese medicine composition of the present invention can regulate the level of male hormones (such as DHT), reduce their binding to hair follicle receptors, and thus inhibit hair follicle atrophy, which is one of the important mechanisms for preventing hair loss. The Chinese medicine composition of the present invention can also inhibit the synthesis of male hormones, block the production of DHT, and protect hair follicles from damage.

[0108] 3. The Chinese medicine composition and shampoo of the present invention contain rich antioxidants, such as vitamins C and E, which can remove free radicals, protect hair follicles from oxidative damage, and promote hair growth; they can also reduce scalp inflammation, improve the hair follicle microenvironment, and are beneficial to hair growth.

[0109] 4. The Chinese medicine composition and shampoo of the present invention have anti-inflammatory effects, can inhibit the release of scalp inflammatory factors, reduce inflammatory reactions, and protect hair follicles; they can also improve scalp microcirculation and promote hair growth.

[0110] 5. The Chinese medicinal composition and shampoo of the present invention can nourish hair roots, improve hair quality, and enhance the elasticity and luster of hair by providing necessary nutrition and nutrients.

[0111] Embodiment 1:

[0112] A traditional Chinese medicine active composition, having the effect of preventing hair loss, comprises the following components (parts by weight):

[0113]

[0114] Its preparation method is:

[0115] Weigh the prescribed amount of Platycladus orientalis leaves, Polygonum multiflorum, Ginseng, Sophora flavescens and Ligusticum chuanxiong and grind them into coarse powder;

[0116] Add the prescribed amount of water, treat with ultrasound (300w, 40KHz) for 30min, and filter with gauze;

[0117] High-speed centrifugation (12000r / min), filtration through a 300-mesh sieve, and constant volume are performed to obtain the anti-hair loss Chinese medicine active composition.

[0118] The anti-hair loss active Chinese medicinal composition is an orange-yellow transparent liquid with a pH value of 5.0-7.0.

[0119] Example 2

[0120] An oil-controlling and anti-hair loss shampoo containing a traditional Chinese medicine active composition consists of phase A, phase B, phase C, phase D and phase F.

[0121] The phase A is divided into phase A1 and phase A2, wherein the raw material of phase A1 includes the following components and the mass percentages of the components are:

[0122]

[0123] The phase A is divided into phase A1 and phase A2, wherein the raw material of phase A2 includes the following components and the mass percentages of the components are:

[0124] Citric acid 0.07%

[0125] Disodium EDTA 0.1%

[0126] The phase B raw material includes the following components and their mass percentages:

[0127] Ammonium Laureth Sulfate 17%

[0128] Ammonium Lauryl Sulfate 4.6%

[0129] The C phase raw material includes the following components and their mass percentages:

[0130] Cetyl alcohol 0.5%

[0131] Cocamide MEA 1%

[0132] Ethylene glycol distearate 1.5%

[0133] The D phase is divided into a D1 phase and a D2 phase, wherein the D1 phase raw material includes the following components and the mass percentages of the components are:

[0134] Cocamidopropyl Betaine 3%

[0135] Piroctone olamine salt 0.3%

[0136] The D phase is divided into a D1 phase and a D2 phase, wherein the D2 phase raw material includes the following components and the mass percentages of the components are:

[0137] Dimethicone, Water, Cocamidopropyl Betaine, Guar Hydroxypropyltrimonium Chloride, C12-15 Pareth-3, Phenoxyethanol 1.8%

[0138] The E phase raw material includes the following components and their mass percentages:

[0139]

[0140] The phase F raw material includes the following components and their mass percentages:

[0141] Sodium citrate 0.05%

[0142] Sodium chloride 0.5%.

[0143] Its preparation method is:

[0144] Step 1: Add the deionized water of phase A1 into the main pot, add the remaining raw materials of phase A1 under homogenization, disperse them completely, add the raw materials of phase A2, and stir well;

[0145] Step 2: Add phase B, heat to 80-85°C with stirring, and stir thoroughly until completely dissolved;

[0146] Step 3: Add phase C and stir until it is completely dissolved, and keep the temperature constant for 20 minutes;

[0147] Step 4: Add the D1 phase raw materials, stir to dissolve completely, and start stirring to cool down

[0148] Step 5: Cool down to 70-75°C, add D2 phase raw materials, stir evenly, and continue to cool down;

[0149] Step 6: Cool down to 45°C, add the raw materials of phase E in sequence, and stir evenly;

[0150] Step 7: Add the ingredients of phase F in sequence, stir until completely dissolved, and obtain the oil-control and anti-hair loss shampoo.

[0151] Embodiment 3:

[0152] An oil-controlling and anti-hair loss shampoo containing a traditional Chinese medicine active composition consists of phase A, phase B, phase C, phase D and phase F.

[0153] The phase A is divided into phase A1 and phase A2, wherein the raw material of phase A1 includes the following components and the mass percentages of the components are:

[0154]

[0155] Preferably, the phase A is divided into phase A1 and phase A2, wherein the raw material of phase A2 includes the following components and the mass percentages of the components are:

[0156] Citric acid 0.05%

[0157] Disodium EDTA 0.05%

[0158] The phase B raw material includes the following components and their mass percentages:

[0159] Ammonium Laureth Sulfate 10%

[0160] Ammonium Lauryl Sulfate 6%

[0161] The C phase raw material includes the following components and their mass percentages:

[0162] Cetyl alcohol 0.8%

[0163] Cocamide MEA 1.5%

[0164] Ethylene glycol distearate 1%

[0165] The D phase is divided into a D1 phase and a D2 phase, wherein the D1 phase raw material includes the following components and the mass percentages of the components are:

[0166] Cocamidopropyl Betaine 2%

[0167] Piroctone olamine salt 0.2%

[0168] The D phase is divided into a D1 phase and a D2 phase, wherein the D2 phase raw material includes the following components and the mass percentages of the components are:

[0169] Dimethicone, water, cocamidopropyl betaine, guar hydroxypropyltrimonium chloride, C12-15 pareth-3, phenoxyethanol 1%;

[0170] The E phase raw material includes the following components and their mass percentages:

[0171]

[0172] The phase F raw material includes the following components and their mass percentages:

[0173] Sodium citrate 0.02%

[0174] Sodium chloride 0.8%.

[0175] The preparation method of the oil-controlling and anti-hair loss shampoo comprises the following steps:

[0176] Step 1: Add the deionized water of phase A1 into the main pot, add the remaining raw materials of phase A1 under homogenization, disperse them completely, add the raw materials of phase A2, and stir well;

[0177] Step 2: Add phase B, heat to 80-85°C with stirring, and stir thoroughly until completely dissolved;

[0178] Step 3: Add phase C and stir until it is completely dissolved, and keep the temperature constant for 20 minutes;

[0179] Step 4: Add the D1 phase raw materials, stir to dissolve completely, and start stirring to cool down

[0180] Step 5: Cool down to 70-75°C, add D2 phase, stir evenly, and continue to cool down;

[0181] Step 6: Cool down to 45°C, add the raw materials of phase E in sequence, and stir evenly;

[0182] Step 7: Add the raw materials of phase F in sequence, stir and dissolve completely to obtain the oil-control and anti-hair loss shampoo.

[0183] Embodiment 4:

[0184] An oil-controlling and anti-hair loss shampoo containing a traditional Chinese medicine active composition consists of phase A, phase B, phase C, phase D and phase F.

[0185] The phase A is divided into phase A1 and phase A2, wherein the raw material of phase A1 includes the following components and the mass percentages of the components are:

[0186]

[0187] Preferably, the phase A is divided into phase A1 and phase A2, wherein the raw material of phase A2 includes the following components and the mass percentages of the components are:

[0188] Citric acid 0.1%

[0189] Disodium EDTA 0.15%

[0190] The phase B raw material includes the following components and their mass percentages:

[0191] Ammonium Laureth Sulfate 20%

[0192] Ammonium Lauryl Sulfate 3%

[0193] The C phase raw material includes the following components and their mass percentages:

[0194] Cetyl alcohol 0.2%

[0195] Cocamide MEA 0.5%

[0196] Ethylene glycol distearate 2%

[0197] The D phase is divided into a D1 phase and a D2 phase, wherein the D1 phase raw material includes the following components and the mass percentages of the components are:

[0198] Cocamidopropyl Betaine 4%

[0199] Piroctone olamine salt 0.5%

[0200] The D phase is divided into a D1 phase and a D2 phase, wherein the D2 phase raw material includes the following components and the mass percentages of the components are:

[0201] Dimethicone, water, cocamidopropyl betaine, guar hydroxypropyltrimonium chloride, C12-15 pareth-3, phenoxyethanol 3%;

[0202] The E phase raw material includes the following components and their mass percentages:

[0203]

[0204] The phase F raw material includes the following components and their mass percentages:

[0205] Sodium citrate 0.08%

[0206] Sodium chloride 0.2%.

[0207] The preparation method of the oil-controlling and anti-hair loss shampoo comprises the following steps:

[0208] Step S1: Add the water of phase A1 into the main pot, add the remaining raw materials of phase A1 under homogenization, disperse them completely, add the raw materials of phase A2, and stir well;

[0209] Step S2: Add phase B, heat to 80-85°C with stirring, and stir thoroughly until completely dissolved;

[0210] Step S3: Add phase C and stir until it is completely dissolved, and keep the temperature constant for 20 minutes;

[0211] Step S4: Add the D1 phase raw material, stir and dissolve completely, start stirring and cooling

[0212] Step S5: Cool down to 70 - 75 °C, add Phase D2, stir evenly, and continue to cool down.

[0213] Step S6: Cool down to 45 °C, add the raw materials of Phase E in sequence, and stir evenly.

[0214] Step S7: Add the raw materials of Phase F in sequence, stir until completely dissolved, and obtain the oil-control and anti-hair-loss shampoo.

[0215] Control Example 1: Minoxidil Liniment; Control Example 2: Market oil-control product; Control Example 3: Market anti-hair-loss product; Control Example 4: Market anti-dandruff product.

[0216] Test Example 1:

[0217] 1.1 Reagents and Drugs

[0218] Example 1 is an anti-hair-loss shampoo of traditional Chinese medicine composition, a hair growth-promoting product developed by the research group of Professor Liu Qiang of Southern Medical University; Testosterone (batch number: RH289029) was purchased from Ron Reagents; Deionized water was自制 by the Traditional Chinese Medicine Preparation Laboratory of Southern Medical University. Control Example 1 Minoxidil Liniment (batch number: 24051912) was purchased from Sichuan Meidakang Pharmaceutical Co., Ltd.

[0219] 1.2 Experimental Instruments

[0220] One-hundred-thousandth analytical balance (sartorius, MSX); Vernier caliper.

[0221] 1.3 Experimental Animals

[0222] C57BL / 6 mice (SPF grade), male, 5 - 6 weeks old, body weight 18 - 22 g, provided by the Experimental Animal Center of Southern Medical University, license number: SCXK (Yue) 2021 - 0041.

[0223] 2 Methods

[0224] 2.1 Establishment and Treatment of AGA Model

[0225] Subcutaneously inject testosterone solution under the dorsal skin of C57BL / 6 mice to induce the AGA model. Subcutaneously inject 0.1 mL of 5 mg / mL testosterone solution into the dorsal skin of each mouse. After 28 days of injection, shave the hair on the back skin with an area of 2 cm × 3 cm, and let the shaved skin recover for 24 h. The mice were randomly divided into 3 groups (n = 6 in each group): (1) Model group: AGA mice were not treated with any formula; (2) The oil-control and anti-hair-loss shampoo group containing the traditional Chinese medicine active composition of this application (Sample 2 group); (3) Minoxidil group (MDX). In addition, 6 healthy mice were set as negative mice.

[0226] 2.2 Inspection of the quality of new hair:

[0227] The days of skin darkening and hair growth in C57BL / 6 male mice in different drug administration groups were observed and recorded every day. The skin of the depilatory area on the back of C57BL / 6 male mice was photographed on days 0, 7, and 14. After 15 days of administration, 15 new hairs were randomly plucked from three different parts of the depilatory area on the back of each C57BL / 6 male mouse, and their lengths were measured with a vernier caliper to calculate the average value; and the hair regrown in the depilatory area on the back was collected and weighed with an analytical balance to calculate the average value. After shaving the hair, the back skin tissue was taken from the depilatory area on the back parallel to the spine, the tissue was fixed in paraformaldehyde, and the H&E staining was followed by microscopic examination and photography to analyze the changes in dermal thickness and the number of hair follicles.

[0228] 2.3 Statistical analysis

[0229] The data were analyzed using SPSS24 statistical software. The experimental data were expressed as mean ± standard deviation (`x±s). One-way analysis of variance was used for comparison among the groups. P < 0.05 indicated a statistically significant difference, and "ns" indicated no statistical difference.

[0230] 3 Results and Discussion

[0231] 3.1 Hair growth in mice

[0232] Hair growth in mice includes the resting phase, the catagen phase, and the growth phase, which is called the hair growth cycle. The color of the hairless skin continues to deepen as the hair follicles transition from the resting phase to the growth phase. The hair follicles of C57BL / 6 mice contain melanocytes, which can synthesize a large amount of melanin during the growth phase, and the skin is black; melanin synthesis decreases during the catagen phase, and the skin is gray; no melanin is synthesized during the resting phase, and the skin is pink. The hair follicles of C57BL / 6 mice at 6-8 weeks old enter the resting phase, so giving testosterone during this period can easily establish an androgenic alopecia model.

[0233] Depend on Figure 1It can be seen that after the modeling, the back skin of the mice was grayish and dull, and the hair growth was in a dormant state. After stimulation with comparative example 1 and anti-hair loss and hair-fixing shampoo, hair growth can enter the growth phase. On the 9th day of administration, the hair on the back of the mice in each medication group did not grow significantly, but the skin color gradually deepened. After 13 days of administration, hair growth tended to be obvious. Only a small part of the model group mice grew hair, while the hair of the mice in the medication group grew to varying degrees, indicating that comparative example 1, anti-hair loss and hair-fixing shampoo can significantly shorten the transition time from the dormant phase to the growth phase of the hair follicles and the time from entering the growth phase to the hair growing out of the skin surface. After 15d, only a small amount of hair grew in the model group mice, and most of the back area was still exposed, the skin was bright white, and the hair growth was in the dormant phase. The hair on the back of the mice in each medication group basically covered the entire back hair loss area, and the color was gray and black and shiny.

[0234] 3.2 Determination of hair length and hair quality

[0235] On the 15th day, all mice grew new hair to varying degrees, and the effect of each formula on hair recovery was evaluated by the length and quality of the new hair in each treatment group. Figure 2 , Figure 3 As shown, compared with the blank group, the hair length and hair quality of the model group were significantly lower than those of the blank group (p<0.05), indicating that the AGA model was successfully established. Compared with the model group, the hair length and hair quality of the minoxidil lotion and anti-hair loss and hair solidifying shampoo were significantly higher than those of the model group after application (p<0.05). The results show that comparative example 1 and anti-hair loss and hair solidifying shampoo have a significant effect on promoting hair growth.

[0236] In summary, there is no significant difference in the effects of the oil-control and anti-hair loss shampoo containing the Chinese medicine active composition and the comparative example 1 (minoxidil lotion) on the hair growth of C57BL / 6 mice, and the effects are equivalent.

[0237] Test Example 2:

[0238] The evaluation was conducted based on human immortalized sebaceous gland cells SZ95. An in vitro oil secretion model was established by stimulating with hormone substances. After drug administration, the oil control efficacy of sample group Example 2 (oil-control and anti-hair loss shampoo containing a traditional Chinese medicine active composition) and comparative example 2 (an oil-control product on the market) was evaluated by evaluating the lipid droplet synthesis trend.

[0239] 1. Main reagents and equipment:

[0240] 1.1 Reagents: MEM culture medium, phosphate buffer, 5α-DHT, linoleic acid, Oil Red O solution;

[0241] 1.2 Instruments: CO2 incubator, clean bench, inverted microscope, centrifuge; 2 Detection methods

[0242] 2.1 Cell inoculation: Dilute the cells to the inoculation density with cell culture medium and inoculate them into 96-well plates, with 200 μL added to each well. After inoculation, place the plates in a CO2 incubator and culture for 24 h ± 2 h.

[0243] 2.2 Dosing treatment: After the cells in the 96-well plate reach an appropriate degree of confluence, the active Chinese medicine composition is prepared according to Example 1. The culture medium in the 96-well plate is discarded, and the culture medium containing the test substance is added to the test substance wells; normal cell culture medium is added to the blank control wells, and the amount of liquid added to each well is 200 μL. After the administration is completed, the 96-well plate is placed in a CO2 incubator and cultured for 24 h±2 h.

[0244] 2.3. Staining: discard the old solution of the 96-well plate, wash the plate twice with buffer solution, add 100 μL of fixative solution to each well to fix the cells; discard the fixative solution, add oil red solution to stain at 37°C for 30 minutes; then discard the oil red solution, add appropriate amount of ultrapure water to wash for 5 minutes; repeat 2-3 times.

[0245] 2.4. Photo observation: Take photos under a microscope and record the staining results, and use image analysis software to calculate the staining area percentage "% Area".

[0246] 2.5. Result analysis: SPSS analysis software was used, and independent sample t-test was used for data statistical analysis between the groups. p<0.05 indicated statistical difference, represented by "*"; p>0.05 indicated no statistical difference.

[0247] 3 Results and conclusions

[0248] Lipid droplet synthesis was detected according to the experimental protocol, and the results of Oil Red staining were as follows Figure 4 As shown, differential analysis of lipid droplet synthesis is as follows Figure 5 The test results show that compared with the blank group, the amount of lipid droplet synthesis in the negative control group increased significantly, indicating that the inducer stimulation in this experiment was effective; when the sample group was administered at a concentration of 0.25 mg / mL, compared with the negative control group, the amount of lipid droplet synthesis in the tested sample group decreased, with a statistically significant difference (p < 0.05), indicating that the Chinese medicine composition anti-hair loss shampoo in Example 2 has the ability to inhibit lipid droplet synthesis at the tested concentration, which can be used as one of the evidences supporting the claim that the Chinese medicine composition anti-hair loss shampoo of the present invention has the effect of controlling oil.

[0249] Lipid droplet synthesis was detected according to the experimental protocol, and the results of Oil Red staining were as follows Figure 6 As shown, differential analysis of lipid droplet synthesis is as follows Figure 7The test results show that compared with the blank group, the amount of lipid droplet synthesis in the negative control group increased significantly, indicating that the inducer stimulation in this experiment was effective; when the sample group was administered at a concentration of 0.25 mg / mL, compared with the negative control group, the amount of lipid droplet synthesis in the tested sample group decreased, with a statistically significant difference (p < 0.05), indicating that Comparative Example 2 has the ability to inhibit lipid droplet synthesis at this tested concentration, which can be used as one of the evidences to support the claim of oil control efficacy.

[0250] In summary, there is no significant difference between the anti-hair loss and hair solidifying shampoo and comparative example 2 in the lipid droplet oil red staining results and lipid droplet synthesis difference analysis, and the shampoo has oil control efficacy and has equivalent effects.

[0251] Test Example 3 In order to test the anti-hair loss effect of the anti-hair loss shampoo proposed in the present invention, an anti-hair loss effect test was conducted in comparison with market products. The test method is:

[0252] Test samples: Comparative Example 2 (oil control product on the market), Comparative Example 3 (anti-hair loss product on the market), Example 2, Example 3, Example 4;

[0253] Through the shampoo counting method: 50 volunteers with oily hair were randomly divided into 10 people / group, and a suitable container was found to collect the fallen hair when washing hair normally. The average number of hairs that fell each time before using the test shampoo was recorded for three consecutive times. Then, the anti-hair loss shampoo was used for 3 weeks, and the number of hairs that fell each time was recorded. The data before and after were compared to see if there was a significant reduction.

[0254] Test results:

[0255]

[0256] Data analysis was performed using Prism 9 statistical software. Paired T test or rank sum test was used to analyze the difference between the baseline and follow-up values ​​of each parameter. "ns" was used to indicate no statistical difference, and "*", "**", "***", and "****" were used to indicate P<0.05, P<0.01, P<0.001, and P<0.0001, respectively.

[0257] Compared with before using the product, the amount of hair loss in comparative example 2 was reduced by 6.67%, and there was no significant difference in hair loss count compared with before using the product (ns) (P>0.05); compared with before using the product, the amount of hair loss in comparative example 3 was reduced by 44.9%, and there was a significant difference in hair loss count compared with before using the product (***) (p<0.001); compared with before using the product, the amount of hair loss in embodiment 2, embodiment 3 and embodiment 4 was reduced by 85.4%, 80.4% and 60.2% respectively, and there was a significant difference in hair loss count compared with before using the product (****) (p<0.0001);

[0258] It can be seen from the above experimental data that the anti-hair loss shampoo of this solution has a significant anti-hair loss effect, and the anti-hair loss shampoo in Example 2 has the best test result after continuous use for 3 weeks.

[0259] Test Example 4: In order to test the anti-dandruff and antipruritic effect of the anti-hair loss shampoo proposed in the present invention, an antibacterial effect test was conducted in comparison with market products, as follows;

[0260] Test samples: Comparative Example 2 (oil control product on the market), Comparative Example 4 (anti-dandruff product on the market), Example 2, Example 3, Example 4:

[0261] Test method: Take 20uL of liquid sample, drip it on the filter paper, dry it and set it aside. Use a sterile cotton swab to dip into a 5.0×10 5 CFU / mL-5.0×10 6 CFU / mL Malassezia furfur suspension was evenly applied on the surface of the appropriate culture medium plate for 3 times. One infected plate was placed on each experiment, and each plate was placed with 4 experimental samples and 1 negative control sample, for a total of 5 plates. Cover the plate, incubate at (36±1)℃ for 72h, observe the results, and measure the diameter of the inhibition ring (including the patch) with a vernier caliper and record it.

[0262] Judgment standard: Negative control samples should not produce antibacterial rings. If the antibacterial diameter of the test sample is >7mm, it is judged to have antibacterial effect, and if the antibacterial diameter is ≤7mm, it is judged to have no antibacterial effect. If there is antibacterial effect in 3 repeated tests (a total of 12 samples), it is judged to be qualified. (The antidandruff effect of the product is characterized by detecting the inhibition rate of Malassezia strains after product treatment through microbial methods).

[0263] Test results:

[0264]

[0265]

[0266] Refer to the enterprise standard "Microbial Antibacterial Evaluation Value In Vitro Inhibition of Bacteria and Fungi Test Operation Instructions (Drug Sensitivity Test)" for the determination criteria of the inhibition zone results: the diameter of the inhibition zone > 20mm is extremely sensitive, expressed as ++++; 15-20mm is highly sensitive, expressed as +++; 10-15mm is moderately sensitive, expressed as ++; <10mm is low sensitivity, expressed as +; 0mm is no antibacterial effect, expressed as -

[0267] In the antidandruff effect of comparative example 2, the antibacterial effect showed low sensitivity; in the antidandruff effect of comparative example 4, the antibacterial effect showed moderate sensitivity; in the antidandruff effect of example 2, the antibacterial effect showed extreme sensitivity; in the antidandruff effect of examples 3 and 4, the antibacterial effect showed high sensitivity

[0268] It can be seen from the above experimental data that the anti-hair loss shampoo of this solution has a significant anti-dandruff effect, while the anti-hair loss shampoo in Example 2 has the best effect in removing dandruff and relieving itching.

[0269] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A Chinese medicine active composition, characterized in that: The Chinese medicine active composition comprises the following components, the mass percentages thereof are:

2. An oil-control and anti-hair loss shampoo containing a Chinese medicine active composition, characterized in that: Including phase A, phase B, phase C, phase D and phase F; The A phase is divided into an A1 phase and an A2 phase, and the A1 phase includes the following components in percentage by weight: The A2 phase includes the following components, and the component mass percentages are: Citric acid 0.05-0.1%; Disodium EDTA 0.05-0.15%; The phase B includes the following components, and the component mass percentages are: Ammonium laureth sulfate 10-20%; Ammonium lauryl sulfate 4-6%; The C phase raw material includes the following components, and the component mass percentages are: Cetyl alcohol 0.2-0.8%; Cocamide MEA 0.5-1.5%; Ethylene glycol distearate 1-2%; The D phase is divided into a D1 phase and a D2 phase, and the D1 phase includes the following components in percentage by mass: Cocamidopropyl Betaine 2-4%; Piroctone olamine salt 0.2-0.5%; The D2 phase includes the following components, and the component mass percentages are: Dimethicone, water, cocamidopropyl betaine, guar hydroxypropyltrimonium chloride, C12-15 pareth-3, phenoxyethanol 1-3%; The E phase includes the following components, and the component mass percentages are: The F phase includes the following components, and the component mass percentages are: Sodium citrate 0.02-0.08%; Sodium chloride 0.2-0.8%.

3. A method for preparing an oil-controlling and anti-hair loss shampoo, characterized in that: The method for preparing the oil-control and anti-hair loss shampoo containing the Chinese medicine active composition as claimed in claim 2 comprises the following steps: Step 1: Add the A1 phase water into the main pot, add the remaining ingredients of the A1 phase under homogenization, disperse completely, add the A2 phase ingredients, and stir thoroughly; Step 2: Add phase B, heat to 80-85°C with stirring, and stir thoroughly until completely dissolved; Step 3: Add phase C and stir until it is completely dissolved, and keep the temperature constant for 20 minutes; Step 4: Add the raw materials of phase D1, stir until completely dissolved, and start stirring to cool down; Step 5: Cool down to 70-75°C, add D2 phase, stir evenly, and continue to cool down; Step 6: Cool down to 45°C, add the raw materials of phase E in sequence, and stir evenly; Step 7: Add the raw materials of phase F in sequence, stir and dissolve completely to obtain the oil-control and anti-hair loss shampoo.

4. The method for preparing the oil-controlling and anti-hair loss shampoo according to claim 3, characterized in that: In step 6, the preparation method of the active Chinese medicine composition in phase E is: Weigh Platycladus orientalis leaves, Polygonum multiflorum, Ginseng, Sophora flavescens and Ligusticum chuanxiong, and crush them into coarse powder; Add water, treat with ultrasound for 30 min, and filter through gauze; The mixture was centrifuged at 12000 r / min, filtered through a 300-mesh sieve, and fixed to volume to obtain a traditional Chinese medicine active composition.

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