A traditional Chinese medicine for clearing fat and strengthening hair, and its preparation method and application

Abstract

The invention discloses a Chinese medicine for clearing fat and strengthening hair tincture, and a preparation method and application thereof, and belongs to the technical field of treating androgenic alopecia. The Chinese medicine for clearing fat and strengthening hair tincture is prepared from Chinese medicinal materials including Platycladus orientalis leaf, Herba schizonepetae, Herba Ecliptae, Folium Mori, Herba Salviae Miltiorrhizae, Herba Taraxaci, Radix Angelicae Sinensis, Radix Astragali, Ganoderma Lucidum, Polygonatum sibiricum, and Rhizoma Curcumae. The preparation method of the Chinese medicine for clearing fat and strengthening hair tincture comprises the steps of crushing 11 Chinese medicinal materials and impregnating under the item of tincture. The Chinese medicine for clearing fat and strengthening hair tincture can promote hair follicles to enter the growth phase in advance, accelerate hair growth, increase the number of hair follicles, improve the miniaturization of hair follicles, reduce serum T level, and increase E2 level. The invention solves the problem of limited selection of clinical treatments for androgenic alopecia, reduces treatment costs, is easy to use, and has few side effects. In addition, the Chinese medicine for clearing fat and strengthening hair tincture prepared by the invention provides a new treatment method for clinical treatment of androgenic alopecia.

Description

Technical Field

[0001] The invention relates to the technical field of treating androgenic alopecia diseases, in particular to a traditional Chinese medicine for clearing fat and strengthening hair, and a preparation method and application thereof. Background Art

[0002] Currently, the U.S. Food and Drug Administration recommends minoxidil and finasteride for the treatment of androgenic alopecia, but the side effects of the above two drugs are obvious. Although laser and surgical treatments can be used for androgenic alopecia, they are expensive and have certain requirements on the operator's skills, and are less popular.

[0003] Chinese herbal medicine can improve hair loss symptoms through multiple targets, multiple pathways, and multiple aspects, and can provide patients with androgenic alopecia with a safe, effective, inexpensive, and convenient treatment method.

[0004] Therefore, the present invention solves the problem of limited selection in clinical treatment of androgenic alopecia. Summary of the invention

[0005] The purpose of the present invention is to provide a traditional Chinese medicine for clearing fat and strengthening hair, and a preparation method and application thereof, which solves the problem of limited selection faced by androgenic alopecia, namely, reduces the cost of treating androgenic alopecia, reduces side effects, is convenient to use, has good therapeutic effects, and the like.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A traditional Chinese medicine for clearing fat and strengthening hair,

[0008] The traditional Chinese medicine for clearing fat and strengthening hair tincture is prepared from Platycladus orientalis leaves, Radix Scutellariae, Herba Ecliptae, Folium Mori, Radix Salviae Miltiorrhizae, Herba Taraxaci, Radix Angelicae Sinensis, Radix Astragali, Ganoderma Lucidum, Polygonatum sibiricum and Curcuma Longa.

[0009] Preferably, the raw materials used to prepare the Qingzhi Gufa tincture are as follows:

[0010] 30 parts of Platycladus orientalis leaves; 30 parts of Herba Polygoni Multiflori; 20 parts of Herba Ecliptae; 30 parts of Folium Mori; 15 parts of Salvia miltiorrhizae; 15 parts of Herba Taraxaci; 15 parts of Radix Angelicae Sinensis; 15 parts of Radix Astragali; 15 parts of Ganoderma Lucidum; 15 parts of Polygonatum sibiricum; 15 parts of Curcuma Longa.

[0011] A method for preparing a traditional Chinese medicine for clearing fat and strengthening hair tincture, wherein the specific steps for preparing the traditional Chinese medicine for clearing fat and strengthening hair tincture are as follows:

[0012] S1: Grinding raw materials: weigh Platycladus orientalis leaves, Herba Polygoni Multiflori, Herba Ecliptae, Folium Mori, Radix Salviae Miltiorrhizae, Herba Taraxaci, Radix Angelicae Sinensis, Radix Astragali, Ganoderma Lucidum, Rhizoma Polygonati Sinensis, and Rhizoma Curcumae Longae, and grind the 11 Chinese medicines into Chinese medicine powder;

[0013] S2: Immersion under the tincture item: use ethanol as a solvent to soak the Chinese medicine powder, stir, immerse, filter and obtain supernatant 1; then immerse the filter residue with ethanol of original concentration, stir, filter and obtain supernatant 2; combine the supernatant 1 and the supernatant 2.

[0014] Preferably, in S1, the 11 Chinese medicinal herbs are ground into powder by screening with a 24-mesh sieve to obtain the Chinese medicinal powder.

[0015] Preferably, the specific steps of S2 are as follows:

[0016] F1: First impregnation: add ethanol 1 to the Chinese medicine powder, shake well, soak for a certain time 1, filter, obtain supernatant 1, and store the residue;

[0017] F2: Second immersion: add ethanol 2 to the filter residue, shake it evenly, soak it for a certain time 2, then filter it to obtain supernatant 2; mix supernatant 1 and supernatant 2, let them stand for a certain time 3, filter them, and the obtained liquid is Qingzhi hair-fixing tincture.

[0018] Preferably, in said S2,

[0019] In F1, the mass of the ethanol 1 added is 6 times the mass of the traditional Chinese medicine powder; the shaking step is to shake once every 12 hours; and the soaking time 1 is 7 days.

[0020] Preferably, in said S2,

[0021] In F2, the mass of the ethanol 2 added is 5 times the mass of the traditional Chinese medicine powder; the shaking step is specifically to shake once every 12 hours, and the soaking time 2 is 7 days.

[0022] Preferably, in said S2,

[0023] The standing time 3 is 24 hours.

[0024] Preferably, in said S2,

[0025] The concentration of ethanol 1 used in F1 and the concentration of ethanol 2 used in F2 were both 70%.

[0026] A traditional Chinese medicine for clearing fat and strengthening hair tincture is used in treating androgenic alopecia.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The main medicines in the Qingzhi Gufa tincture of the present invention are two Chinese medicines, Platycladus orientalis leaf and Radix Scutellariae, and the flavonoids contained in the two are the main effective ingredients for promoting hair growth. The present invention can prompt hair follicles to enter the growth phase in advance, accelerate hair growth, increase the number of hair follicles, improve hair follicle miniaturization, reduce serum T levels and increase E2 levels. Therefore, the problem of limited selection of clinical treatment of androgenic alopecia is solved, and a tincture with greater efficacy can be exerted under the condition of low economic cost, and it is easy to use and has little side effects, which can reduce the psychological and economic burden of patients in treating androgenic alopecia, reduce the medical expenses of the disease, and provide a new treatment method for clinical treatment of androgenic alopecia. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 is the liquid chromatogram of quercetin reference substance;

[0030] Figure 2 is the liquid chromatogram of the negative sample;

[0031] Figure 3 is the liquid chromatogram of the test product;

[0032] Figure 4 is the linear range diagram of quercetin;

[0033] Figure 5 The hair growth charts of each group of mice at 1 week, 3 weeks, and 6 weeks;

[0034] Figure 6 HE staining of the back skin of mice in each group (×100). DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. The experimental methods described in the following embodiments are conventional methods unless otherwise specified; the reagents and materials, unless otherwise specified, can be obtained from commercial channels.

[0036] Example

[0037] Preparation of lipid-clearing and hair-strengthening tincture:

[0038] Weigh 30g of Platycladus orientalis leaf, 30g of Radix Angelicae Pubescentis, 20g of Herba Ecliptae, 30g of Folium Mori, 15g of Salvia miltiorrhizae, 15g of Taraxacum mongolicum, 15g of Angelica sinensis, 15g of Astragalus, 15g of Ganoderma lucidum, 15g of Polygonatum sibiricum, and 15g of Curcuma longa, and crush the 11 Chinese medicines in the prescription into coarse powder. According to the immersion method under the tincture item (Tincture 0120 of the Fourth General Rules of the 2020 Edition of the Chinese Pharmacopoeia), use ethanol of appropriate concentration as solvent, seal, stir, immerse for an appropriate time, filter the supernatant, dilute to the specified amount with ethanol of the original concentration, immerse for an appropriate time, combine the filtrate, stir evenly, let stand overnight, filter, and can. The 11 Chinese medicines in the prescription are crushed into coarse powder, which is the Chinese medicine powder obtained after sieving with a 24-mesh sieve.

[0039] The factors of investigation were the amount of ethanol, ethanol concentration and immersion time. Three different levels were selected for each factor. The dry extract yield and quercetin extraction amount were used as the investigation indicators. The orthogonal experimental method was used. The results were as follows: 4 ) Orthogonal table was used to arrange the experiment and determine the appropriate preparation process. See Table 1 for details. The ethanol dosage and immersion time in each level are given as two values, which are in accordance with the tincture immersion method specified in the pharmacopoeia. The immersion is required for 2 times, and the ethanol concentration used is unchanged.

[0040] Table 1 Orthogonal test factor levels

[0041]

[0042] The dry paste yield was calculated using the following formula:

[0043] Dry paste yield = (dry paste weight × total volume of medicinal solution) / (sampling volume × amount of crude drug) × 100%;

[0044] The quercetin content was determined by high performance liquid chromatography, and the calculation formula is as follows:

[0045] The amount of quercetin extracted = the total volume of the drug solution × the concentration of the component in the drug solution.

[0046] Example Results

[0047] (1) Orthogonal test results

[0048] The calculation results of the dry extract yield and quercetin extraction amount of 9 batches of samples in the orthogonal experiment are shown in Tables 2 and 3.

[0049] Table 2 Orthogonal experiment results

[0050]

[0051] Table 3 Results of variance analysis

[0052]

[0053]

[0054] Note: “**” indicates a very significant difference, “*” indicates a significant difference, F 0.05 (2, 2) = 19.00, F 0.01 (2, 2) = 99.00

[0055] From the results of variance analysis of dry cream yield, we can see that: A factor has significant difference, and B and C factors have no significant difference. Among A factors, A1>A2>A3, B factors B3>B2>B1, C factors C1>C3>C2, and A1B3C1 combination is the best. From the results of variance analysis of quercetin extraction, we can see that: B factor has significant difference, A and C factors have no significant difference, A factor A1>A2>A3, B factor B3>B2>B1, C factor C1>C3>C2, and A1B3C1 combination is the best. The above orthogonal results show that the dry cream yield factor A has significant difference, indicating that the amount of ethanol added has a significant effect on it; the quercetin extraction factor B has significant difference, indicating that the ethanol concentration has a significant effect on it. Considering comprehensively, the A1B3C1 combination is the best for this product. The best process is: add 70% ethanol to soak twice, each time for 7 days, shake once every 12 hours, add 6 times the amount of ethanol to soak for 7 days, filter, and reserve the filtrate; add 5 times the amount of ethanol to soak for another 7 days, filter, combine the filtrate, stir, let stand, filter, and get it. This option has not been tested in the above orthogonal test and needs to be verified by orthogonal test.

[0056] (2) Orthogonal Validation

[0057] Weigh 71.67g of the slices according to the prescription ratio, soak twice in 70% ethanol, each time for 7 days, shake once in the morning and evening every day, add 6 times the amount of ethanol for the first time, soak for 7 days, filter, and use the filtrate for later use; add 5 times the amount of ethanol for the second time, soak for another 7 days, filter, combine the filtrates, stir well, let stand overnight, and filter. The results are shown in Table 4.

[0058] Table 4 Orthogonal validation results

[0059]

[0060] The experimental results show that the dry paste yield and quercetin extraction amount of the three batches of verification samples are similar, indicating that the optimal extraction process conditions are feasible and can be used as the alcohol extraction process for the fat-clearing and hair-fixing tincture.

[0061] (3) Methodological results

[0062] ① Exclusiveness

[0063] Figure 1 , Figure 2 , Figure 3The results showed that the negative sample chromatographic peak had no corresponding chromatographic peak at the corresponding position of the reference and test sample chromatographic peaks, indicating that the negative sample had no interference.

[0064] Preparation of reference solution: Accurately weigh 10.02 mg of quercetin reference substance (provided by China Food and Drug Inspection Institute, batch number 111538-202007, content is 93.5%, for content determination) and place it in a 50 ml volumetric flask, add appropriate amount of methanol to dissolve by ultrasonic and dilute to the scale, shake well to obtain the mother solution. Accurately take 5 ml of the mother solution and place it in a 20 ml volumetric flask, add methanol to dilute to the scale, shake well to obtain.

[0065] Preparation of test solution: Take 9 portions of test solution under the orthogonal item, filter through a 0.45μm microporous filter membrane, and directly inject the sample to obtain the solution.

[0066] Preparation of negative sample solution: After consulting relevant literature, it was found that Platycladus orientalis leaves, Herba schonoskiae radix, and Morus alba leaves contained quercetin among the 11 herbs in the prescription. Therefore, a negative sample solution lacking Platycladus orientalis leaves, Herba schonoskiae radix, and Herba schonoskiae radix was prepared according to the optimal process scheme.

[0067] ②Linear range

[0068] The standard curve was drawn with the reference substance concentration as the horizontal axis and the peak area as the vertical axis. The regression equation was: Y = 28.156X + 81.53, r = 0.9997. The results showed that quercetin had a good linear relationship in the range of 10.02 to 160.32 μg / ml. The results are shown in Table 5. Figure 4 .

[0069] Table 5 Preparation of linear quercetin reference solution

[0070]

[0071] ③Precision

[0072] The results show that the instrument has good precision. See Table 6 for details.

[0073] Table 6 Precision test results

[0074]

[0075] ④Repeatability

[0076] The results show that this method has good repeatability. See Table 7 for details.

[0077] Table 7 Repeatability test results

[0078]

[0079] ⑤ Solution stability

[0080] The results showed that the test solution had no significant changes within 24 hours and had good stability. See Table 8 for details.

[0081] Table 8 Stability test results

[0082]

[0083] ⑥ Sample recovery test

[0084] Referring to the method and regulations under Item 9101 of Part IV of the 2020 edition of the Chinese Pharmacopoeia, the results show that the sample recovery rate of this product is good. See Table 9 for details.

[0085] Table 9 Sample recovery determination results

[0086]

[0087] This embodiment studies the preparation process of the fat-clearing and hair-fixing tincture as the main content, combines the characteristics of the tincture, establishes scientific evaluation indicators, namely the dry paste yield and the quercetin extraction amount, and adopts the orthogonal test method to optimize the best impregnation method.

[0088] The best screening process is:

[0089] Soak twice in 70% ethanol, 7 days each time. The specific steps are as follows:

[0090] F1: The first soaking of Chinese medicine powder is to add 6 times the amount of ethanol (weight of dry Chinese medicine powder), shake once every 12 hours, shake well, soak for 7 days and filter, the filtrate is reserved, and the filter residue is stored;

[0091] F2: The second soaking is to add 5 times the amount of ethanol (weight of dry Chinese medicine powder) to the filter residue, shake it once every 12 hours, soak it for 7 days, and filter it; add the filtrate obtained in step F1 to the obtained filtrate, stir it evenly, let it stand for 24 hours, and filter it to obtain the product.

[0092] The method has simple preparation and reasonable process, and can produce lipid-clearing and hair-fixing tincture with stable quality, providing a basis for subsequent research.

[0093] Application Examples

[0094] In the embodiment, the best preparation method is screened out with the quercetin extraction amount and the dry paste yield as indicators. The following experiment is carried out by preparing the fat-clearing and hair-fixing tincture using the best preparation process. Then, on this basis, the conditions remain unchanged, and the amount of crude drugs prepared by the best process is halved as a low-concentration fat-clearing and hair-fixing tincture, and the amount of crude drugs prepared by the best process is doubled as a high-concentration fat-clearing and hair-fixing tincture.

[0095] Animal experiments were conducted to study the effect of the Zhiqing Gufa tincture prepared under the optimal preparation process on hair growth in the androgenic alopecia mouse model. 70 SPF-grade C57BL / 6 mice were randomly divided into 7 groups, namely, a blank group, a model group, an ethanol group, a minoxidil tincture group, and low, medium, and high concentration Zhiqing Gufa tincture groups. Testosterone propionate injection (5 mg / kg / d) was subcutaneously injected for 4 consecutive weeks to establish an androgenic alopecia mouse model. After the model was successfully established, testosterone propionate was continued to be subcutaneously injected in the hair removal area while applying the corresponding drugs. The blank group and the model group were smeared with normal saline, 1 mL / (mouse·time), once a day, for 6 consecutive weeks. The specific dosing conditions of each group of mice are shown in Table 10.

[0096] Table 10 Drug administration of mice in each group

[0097]

[0098] The hair growth of mice was observed with the naked eye, and the time of darkening of the skin color in the depilatory area was recorded. Five new hairs were randomly plucked on the 14th, 21st, 28th, 35th, and 42nd days of drug intervention, and their lengths were measured and the average value was calculated. The morphology of hair follicles in the depilatory area was observed by HE staining, and the number of hair follicles and the ratio of terminal hair to vellus hair were calculated. The levels of testosterone and estradiol in serum were detected by ELISA, and the ratio between the two was calculated.

[0099] Results and Discussion

[0100] (1) Hair growth in mice

[0101] In the first week after the start of drug intervention, it can be observed that the blank group and minoxidil tincture group can see obvious hair growth; hair growth can also be seen in the concentration groups of Qingzhi Gufa tincture, but the overall situation is worse than the blank group and minoxidil tincture group, and there is no hair growth in some areas; scattered sparse hair can be seen in the model group and ethanol group, and there is no hair growth in most areas. In the third week of intervention, the hair of the blank group mice almost completely covered the hair loss area, and the hair color was black and shiny; the minoxidil tincture group and the high-concentration Qingzhi Gufa tincture group had a high hair coverage rate, and the low and medium concentration Qingzhi Gufa tincture groups had a lower hair coverage rate than the high concentration group; the hair growth of the model group and ethanol group mice was still sparse, and there was no hair growth in some areas. At the 6th week of intervention, the mice in the blank group, minoxidil tincture group, and high-concentration Qingzhi Gufa tincture group had almost complete hair coverage, with shiny black hair; the low- and medium-concentration Qingzhi Gufa tincture groups had more vigorous hair growth, but there were still some areas with sparse hair growth; the model group and ethanol group had sparse hair growth overall, with dull gray hair, and no hair growth in some areas. Figure 5 .

[0102] (2) The time it takes for the skin color of the mouse hair loss area to turn dark

[0103] The time of skin color darkening in the hair removal area of ​​mice in each group did not conform to the normal distribution (P<0.05). The Kruskal-Wallis one-way ANOVA test of non-parametric test was used for multiple group comparison. The results showed that there were statistical differences in the time of skin color darkening in the hair removal area of ​​mice in each group (P<0.01). Further pairwise comparisons were performed. After Bonferroni correction, the results showed that compared with the blank group, the time of skin color darkening in the hair removal area of ​​mice in the model group was significantly delayed (P<0.01); compared with the model group, the time of skin color darkening in the high-concentration Qingzhi Gufa Tincture group and the minoxidil tincture group was advanced (P<0.05), and there was no significant difference in the ethanol group, low-concentration Qingzhi Gufa Tincture group and medium-concentration Qingzhi Gufa Tincture group (P>0.05); compared with the minoxidil tincture group, there was no significant difference in the concentration groups of Qingzhi Gufa Tincture (P>0.05). The results are shown in Table 11.

[0104] Table 11 The time of skin color darkening in each group of mice (P25, P75)

[0105]

[0106] Note: Compared with the blank group, **P<0.01; compared with the model group, ## P<0.01

[0107] (3) Length of new hair in mice

[0108] The data of each group were in line with the normality and variance homogeneity test (P>0.05), so a one-way repeated measures analysis of variance was used. The data did not meet the football symmetry (P<0.05). After adjustment by the GG method, the within-subject effect analysis showed that the time point factor F=5002.990, P<0.01, and the interaction between time point and grouping F=9.353, P<0.01, were statistically significant, indicating that the length of new hair of mice was different at different time points, and the different intervention methods in each group had a statistically significant effect on the length of new hair of mice. The between-subject effect analysis showed that F=530.319, P<0.01, were statistically significant, indicating that there were differences in the effect of increasing the length of new hair of mice in each group.

[0109] Intra-group comparison: The results of multiple comparison mean values ​​showed that the length of new hair gradually increased with the extension of intervention time. The blank group, model group, ethanol group, minoxidil tincture group, and high-concentration clearing and hair-fixing tincture group had statistically significant differences in the length of new hair at each time point (P<0.01). There was no significant statistical difference between the low-concentration and medium-concentration clearing and hair-fixing tincture groups between the 14th and 21st days of intervention (P>0.05), and there were statistical differences between the other time points (P<0.01).

[0110] Comparison among groups: Compared with the blank group, the length of new hair in the model group was significantly shortened at each time point (P<0.01). Compared with the model group, there was no significant difference in the ethanol group (P>0.05), and there was no significant difference in the low-concentration Qingzhi Gufa Tincture group at 14 days and 21 days of intervention (P>0.05), while the length of new hair in the medium-concentration and high-concentration groups increased significantly at each time point (P<0.01). Compared with the minoxidil tincture group, there was no significant difference in hair length in the medium-concentration group at 14 days of intervention and in the high-concentration group at 14 days, 21 days, and 42 days of intervention (P>0.05). At the other time points, the hair length of each concentration group of Qingzhi Gufa Tincture was shorter than that of the minoxidil tincture group (P<0.05). There was no significant difference among the concentration groups of Qingzhi Gufa Tincture at 14 days of intervention (P>0.05). At 21 days and 28 days of intervention, there was still no significant difference between the low and medium concentration groups (P>0.05), but the hair length of the high concentration group was significantly longer than that of the low and medium concentration groups (P<0.01). At 35 days and 42 days of intervention, there were significant differences in hair length among the three groups (P<0.01), and the hair length from long to short was high, medium, and low concentration groups. The results are shown in Table 12.

[0111] Table 12 Comparison of new hair length in each group of mice at different time points (mm)

[0112]

[0113] Note: There is no statistically significant difference between the two groups with the same letter.

[0114] (4) HE staining of mouse back skin (100x)

[0115] In the blank group, a large number of hair follicles were observed, with active hair follicle proliferation, high hair follicle density, and dark hair shaft pigmentation; in the model group and ethanol group, hair follicles were sparse, hair follicle density was significantly reduced, hair shaft pigmentation was light or absent, and hair follicle miniaturization was serious; compared with the model group, the minoxidil tincture group, high-concentration and medium-concentration Qingzhi Gufa tincture group had higher hair follicle density than before, obvious hair shaft melanin formation, and improved hair follicle miniaturization, while the overall improvement of hair follicles in the low-concentration Qingzhi Gufa tincture group was worse than that in the high-concentration and medium-concentration groups. Figure 6 .

[0116] (5) Number of hair follicles and ratio of terminal hair to vellus hair in each group of mice

[0117] All data were normal and homogeneous in variance. According to the results of one-way analysis of variance, P < 0.01, it cannot be assumed that the overall means among the groups were the same, and the LSD test was used for pairwise comparisons. The results showed that compared with the blank group, the number of hair follicles in the model group was significantly reduced (P<0.01), and the ratio of terminal hair to vellus hair was significantly reduced (P<0.01); compared with the model group, there were no significant differences in the number of hair follicles and the ratio of terminal hair to vellus hair in the ethanol group and the low-concentration Qingzhi Gufa Tincture group (P>0.05), but the number of hair follicles in the minoxidil tincture group, the medium-concentration Qingzhi Gufa Tincture group and the high-concentration Qingzhi Gufa Tincture group increased significantly (P<0.01), and the ratio of terminal hair to vellus hair increased significantly (P<0.01); compared with the minoxidil tincture group, the number of hair follicles in each concentration group of Qingzhi Gufa Tincture was significantly reduced (P<0.01), and the ratio of terminal hair to vellus hair was significantly reduced (P<0.01); there were no significant differences in the number of hair follicles and the ratio of terminal hair to vellus hair between the medium-concentration Qingzhi Gufa Tincture group and the high-concentration group (P>0.05), but both groups were significantly greater than the low-concentration group (P<0.01). The results are shown in Table 13.

[0118] Table 13 The number of hair follicles and the ratio of terminal hair to vellus hair in each group of mice

[0119]

[0120] Note: Compared with the blank group, **P<0.01; compared with the model group, ## P<0.01; compared with the minoxidil tincture group, ▲▲ P<0.01

[0121] (6) Testosterone (T), estradiol (E2) levels and T / E2 ratio in mouse serum

[0122] The hormone data of mice in each group were in accordance with normal distribution and homogeneity of variance. According to the results of one-way analysis of variance, P<0.01 could not be considered that the overall means of the groups were the same. LSD test was used for pairwise comparison. The results showed that compared with the blank group, the T level in the serum of mice in the model group was significantly increased (P<0.01), the E2 level was significantly decreased (P<0.01), and the T / E2 ratio was significantly increased (P<0.01); compared with the model group, there were no significant differences in T, E2 and T / E2 ratio in the serum of mice in the ethanol group (P>0.05), while there were significant differences in the minoxidil tincture group (P<0.01). The T level of each concentration group of Qingzhi Gufa tincture was reduced (P<0.05), and the T / E2 ratio was significantly reduced (P<0.01). The E2 level in the medium and high concentration groups was significantly increased (P<0.01), and the low concentration group was significantly increased (P<0.01). There was no significant difference in E2 level between the two groups (P>0.05); compared with minoxidil tincture, there was no significant difference in T, E2 and T / E2 ratio between the high-concentration Qingzhi Gufa tincture group (P>0.05), but the T level in the medium and low concentration groups was significantly increased (P<0.01), the E2 level was significantly decreased (P<0.01), and the T / E2 ratio was significantly increased (P<0.01); there were differences in T, E2 and T / E2 ratio between the two concentration groups of Qingzhi Gufa tincture (P<0.05), the T level and T / E2 ratio were low, medium and high concentration groups from large to small, and the E2 level was high, medium and low concentration groups from large to small. The results are shown in Table 14.

[0123] Table 14 Serum T, E2 levels and T / E2 ratios of mice in each group

[0124]

[0125] Note: Compared with the blank group, **P<0.01; compared with the model group, # P<0.05, ## P<0.01; compared with the minoxidil tincture group, ▲▲ P<0.01

[0126] The above experiments show that the Qingzhi Gufa tincture can promote hair follicles to enter the growth phase in advance, accelerate hair growth, increase the number of hair follicles, improve hair follicle miniaturization, reduce serum T levels and increase E2 levels, which may improve androgenic alopecia (AGA) by regulating hormone levels. The Qingzhi Gufa tincture of the present invention was used, and no adverse reactions were observed in the hair loss area of ​​mice after medication during the experiment, so the Qingzhi Gufa tincture has almost no side effects.

[0127] The fat-clearing and hair-fixing tincture of the present invention is extracted by soaking 11 Chinese medicines such as Platycladus orientalis, Herba Scutellariae, Herba Ecliptae, and Salvia miltiorrhizae in an ethanol solution. Tincture is an important component of Chinese medicine dosage forms. Ethanol is not only conducive to the precipitation of effective ingredients of Chinese medicine, but also can dissolve sebum to a certain extent to improve the greasy scalp condition. Therefore, Chinese medicine tincture is often used in clinical treatment of androgenic alopecia. The monarch medicine in the fat-clearing and hair-fixing tincture is Platycladus orientalis and Herba Scutellariae. Modern research confirms that the flavonoids contained in the two are the main effective ingredients for promoting hair growth, and the determination of quercetin content is a more commonly used determination index for flavonoid extraction technology and quality standards. In addition, the fat-clearing and hair-fixing tincture also contains multiple ingredients such as tanshinone, volatile oil, echinocystic acid, butein, and clover, which may play a role in promoting androgenic alopecia by regulating hormone levels, inhibiting SRD5A activity, anti-inflammatory and antibacterial, improving blood circulation, etc.

[0128] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A Chinese medicine for clearing fat and strengthening hair, characterized in that: The traditional Chinese medicine for clearing fat and strengthening hair tincture is prepared from Platycladus orientalis leaves, Radix Scutellariae, Herba Ecliptae, Folium Mori, Radix Salviae Miltiorrhizae, Herba Taraxaci, Radix Angelicae Sinensis, Radix Astragali, Ganoderma Lucidum, Polygonatum sibiricum and Curcuma Longa.

2. A Chinese medicine for clearing fat and strengthening hair according to claim 1, characterized in that: The raw materials used to prepare the Qingzhi Gufa tincture are as follows: 30 parts of Platycladus orientalis leaves; 30 parts of Radix Scutellariae; 20 parts of Herba Ecliptae; 30 parts of Folium Mori; 15 parts of Radix Salviae Miltiorrhizae; 15 parts of Herba Taraxaci; 15 parts of Radix Angelicae Sinensis; 15 parts of Radix Astragali; 15 servings of Lingzhi; 15 parts of Polygonatum sibiricum; 15 parts of turmeric.

3. The method for preparing the fat-clearing and hair-fixing tincture Chinese medicine according to claim 1, characterized in that: The specific steps of preparing the Chinese medicine for clearing fat and strengthening hair tincture are as follows: S1: Grinding raw materials: weigh Platycladus orientalis leaves, Herba Polygoni Multiflori, Herba Ecliptae, Folium Mori, Radix Salviae Miltiorrhizae, Herba Taraxaci, Radix Angelicae Sinensis, Radix Astragali, Ganoderma Lucidum, Rhizoma Polygonati Sinensis, and Rhizoma Curcumae Longae, and grind the 11 Chinese medicines into Chinese medicine powder; S2: Immersion under the tincture item: use ethanol as a solvent to soak the Chinese medicine powder, stir, immerse, filter and obtain supernatant 1; then immerse the filter residue with ethanol of original concentration, stir, filter and obtain supernatant 2; combine the supernatant 1 and the supernatant 2.

4. The method for preparing the fat-clearing and hair-fixing tincture according to claim 3, characterized in that: In S1, the 11 Chinese medicinal herbs are ground into powders which are obtained by sieving them using a 24-mesh sieve.

5. The method for preparing the fat-clearing and hair-fixing tincture according to claim 3, characterized in that: The specific steps of S2 are as follows: F1: First impregnation: add ethanol 1 to the Chinese medicine powder, shake well, soak for a certain time 1, filter, obtain supernatant 1, and store the residue; F2: Second immersion: add ethanol 2 to the filter residue, shake it evenly, soak it for a certain time 2, then filter it to obtain supernatant 2; mix supernatant 1 and supernatant 2, let them stand for a certain time 3, filter them, and the obtained liquid is Qingzhi hair-fixing tincture.

6. The method for preparing the fat-clearing and hair-fixing tincture according to claim 5, characterized in that: In said S2, In F1, the mass of the ethanol 1 added is 6 times the mass of the traditional Chinese medicine powder; the shaking step is to shake once every 12 hours; and the soaking time 1 is 7 days.

7. The method for preparing the fat-clearing and hair-fixing tincture according to claim 5, characterized in that: In said S2, In F2, the mass of the ethanol 2 added is 5 times the mass of the traditional Chinese medicine powder; the shaking step is specifically to shake once every 12 hours, and the soaking time 2 is 7 days.

8. The method for preparing the fat-clearing and hair-fixing tincture according to claim 5, characterized in that: In said S2, The standing time 3 is 24 hours.

9. The method for preparing the fat-clearing and hair-fixing tincture according to claim 5, characterized in that: In said S2, The concentration of ethanol 1 used in F1 and the concentration of ethanol 2 used in F2 were both 70%.

10. The fat-clearing and hair-fixing tincture Chinese medicine as claimed in claim 1 or the fat-clearing and hair-fixing tincture Chinese medicine as prepared by the preparation method of the fat-clearing and hair-fixing tincture Chinese medicine as claimed in claim 3, characterized in that: The traditional Chinese medicine of Qingzhi Gufa tincture is used in treating androgenic alopecia.

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