A kind of lipopeptide amino acid water-soluble fertilizer and preparation method thereof

Abstract

A lipopeptide amino acid water-soluble fertilizer and a preparation method thereof of the present invention belong to fertilizers. The lipopeptide amino acid water-soluble fertilizer is made of 5 to 12 parts of lipopeptide surfactant stock solution, 10 to 20 parts of chelated medium and trace element mother solution, 15 to 20 parts of composite amino acid solution, 5 to 10 parts of urea solution, 0.1 to 0.5 parts of pollen polysaccharide, and 37.5 to 64.9 parts of water, by weight. The preparation method is to dissolve a total of 15 parts of composite amino acids in 40 parts of water to obtain a composite amino acid solution, dissolve 10 parts of urea in 20 parts of water to obtain a urea solution, and then add lipopeptide surfactant stock solution and pollen polysaccharide after mixing the composite amino acid solution, medium and trace element mother solution, and urea solution, and then add water to make up the volume after mixing evenly, and filter to remove non-water-soluble impurities after stirring until dissolved, so as to obtain lipopeptide amino acid water-soluble fertilizer. The lipopeptide surfactant stock solution added in the present invention can improve the fertilizer absorption efficiency, and the surfactant can be directly absorbed and utilized by plants as nutrients; pollen polysaccharide can improve fertilizer efficiency, so as to achieve the purpose of saving fertilizer and increasing efficiency.

Description

Technical Field

[0001] The invention belongs to fertilizers, and particularly relates to a lipopeptide amino acid water-soluble fertilizer and a preparation method thereof. Background Art

[0002] Foliar fertilization has been widely used as a highly efficient foliar fertilization technology. However, due to the rough structure of the plant surface, most leaves are hydrophobic, resulting in serious losses of the spray solution. When foliar fertilization is carried out in agricultural production, many factors affect the efficiency of fertilizer absorption, such as the degree of stomatal opening, the thickness of the wax layer and cuticle, and the evaporation of fertilizer droplets. Adding surfactants to foliar fertilizers can promote the absorption of nutrients by crops, but traditional surfactants are difficult to degrade by biological methods, forming long-term residues, which seriously affect the environmental ecosystem. Summary of the invention

[0003] The present invention aims to overcome the deficiencies of the prior art and provides a lipopeptide amino acid water-soluble fertilizer and a preparation method thereof.

[0004] The lipopeptide amino acid water-soluble fertilizer of the present invention is prepared from the following components by weight: 5-12 parts of lipopeptide surfactant stock solution, 10-20 parts of chelated trace element mother solution, 15-20 parts of composite amino acid solution, 5-10 parts of urea solution, 0.1-0.5 parts of pollen polysaccharide and 37.5-64.9 parts of water.

[0005] As a further improvement of the present invention, the composite amino acid is any one or more of arginine, leucine, glutamic acid, proline, glycine, alanine, histidine, aspartic acid, lysine, serine, phenylalanine and tyrosine in any proportion.

[0006] As a further improvement of the present invention, the content of the lipopeptide surfactant in the lipopeptide surfactant stock solution is ≥1.5 g / L.

[0007] As a further improvement of the present invention, the chelated trace element mother solution is prepared by the following method; (1) Add 4-7 parts of EDTA-2Na to 63-74 parts of water, stir and dissolve to obtain a chelating solution; (2) Add one or more of magnesium sulfate, magnesium chloride, ferrous sulfate, ammonium molybdate, copper sulfate, zinc sulfate, sodium borate, manganese sulfate, and calcium chloride to the chelating solution, stir and heat to 40-60° C., maintain for 2 hours, and obtain a mother solution of medium and trace elements.

[0008] As a further improvement of the present invention, the added amounts of the magnesium sulfate, magnesium chloride, ferrous sulfate, ammonium molybdate, copper sulfate, zinc sulfate, sodium borate, manganese sulfate and calcium chloride are 2 to 10 parts respectively.

[0009] A method for preparing a lipopeptide amino acid water-soluble fertilizer of the present invention comprises the following steps: (1) Weighing the composite amino acid according to the mass fraction, dissolving a total of 15 parts of the composite amino acid in 40 parts of water to obtain a composite amino acid solution; (2) Weighing urea by mass, dissolving 10 parts of the urea in 20 parts of water to obtain a urea solution; (3) After mixing the composite amino acid solution, the mother solution of trace elements and the urea solution, the lipopeptide surfactant solution and the pollen polysaccharide are added, the mixture is evenly mixed and then water is added to make up the volume. After stirring until dissolved, the non-water-soluble impurities are filtered out to obtain the lipopeptide amino acid water-soluble fertilizer.

[0010] Lipopeptide surfactants are cyclic lipopeptide compounds synthesized by Bacillus through a unique non-ribosomal peptide pathway. They have the advantages of strong surface activity, strong environmental compatibility, low toxicity and good biodegradability. Studies have shown that the application of lipopeptide surfactants can promote crop growth, mainly because lipopeptide surfactants can easily enter the inner part of the leaves through the leaf surface, and high molecular weight surfactant can be decomposed into small molecular organic substances such as amino acids and fatty acids under the action of phospholipids, which can not only directly provide energy for crop metabolism, but also provide a skeleton for the synthesis of lipids, proteins and other substances.

[0011] Pollen polysaccharide is an active substance extracted from pollen residue using enzymatic hydrolysis, targeted extraction and other processes. It contains polysaccharides, amino acids, peptides, flavonoids, lipids, phenols, vitamins and other nutrients, and has the effects of promoting root growth, strengthening seedlings and increasing yields. Since pollen polysaccharides have poor mobility in the soil, the effectiveness of using them as base fertilizer particles or water-soluble fertilizers for flushing is low. Adding them to water-soluble fertilizers containing amino acids for foliar fertilizer spraying can quickly supplement comprehensive nutrition for crops and promote crop growth.

[0012] The solubility of pollen polysaccharides in amino acid-containing water-soluble fertilizers is a key indicator that determines whether an effective amount of addition can be achieved. Adding lipopeptide concentrate to pollen polysaccharides can improve the solubility of pollen polysaccharides. Combining lipopeptides with pollen polysaccharides as synergistic ingredients for amino acid-containing water-soluble fertilizers can form a new type of high-efficiency amino acid-containing water-soluble fertilizer from two directions: improving fertilizer absorption efficiency and increasing fertilizer effective ingredients.

[0013] Compared with the prior art, the present invention has the following beneficial effects: 1. The lipopeptide amino acid water-soluble fertilizer of the present invention can be absorbed and utilized by crops by adding the lipopeptide surfactant stock solution, which plays the role of reducing the surface tension of the fertilizer, increasing the stomatal opening, increasing the wetting area of ​​the fertilizer on the crop leaves and increasing the permeability of nutrients, thereby expanding the molecular channel and weakening the barrier of molecular entry, deepening the absorption degree of insoluble trace elements such as boron and copper, improving the fertilizer absorption efficiency, saving fertilizer and increasing efficiency; 2. The surfactant added in the present invention is a biosurfactant produced by the metabolism of Bacillus subtilis, which has the characteristics of strong environmental compatibility, low toxicity, and can be directly absorbed and utilized by plants as nutrients; 3. Pollen polysaccharides have the effects of promoting root growth, strengthening seedlings, and improving crop quality. The combination of pollen polysaccharides and lipopeptides can effectively improve the solubility of pollen polysaccharides, increase the dosage of pollen polysaccharides, improve fertilizer efficiency, and achieve the purpose of saving fertilizer and increasing efficiency. DETAILED DESCRIPTION

[0014] Example 1

[0015] The lipopeptide amino acid water-soluble fertilizer of the present invention is prepared from the following raw materials in parts by weight: 10 parts of lipopeptide surfactant stock solution, 18 parts of chelated trace element mother solution, 18 parts of composite amino acid solution, 9 parts of urea solution, 0.3 parts of pollen polysaccharide and 44.7 parts of water.

[0016] Among them, the preparation method of the lipopeptide surfactant stock solution is as follows: Preparation of lipopeptide surfactant: transfer the preserved strain to the LB plate and culture for 24 hours; activate Bacillus subtilis ACCC01430 and inoculate it in the seed tank, culture it at 30°C for 24 hours, and obtain a seed solution of suitable seed age. 3% of the seed solution is inoculated into the liquid culture medium, fermented and cultured at 30°C for 38h-48h, and the constant culture pH is between 6.8-7.2. If the content of the lipopeptide surfactant is determined to be above 1.5g / L, the culture can be terminated. The fermentation broth is the lipopeptide surfactant stock solution, the lipopeptide surfactant content is ≥1.5g / L, and the surface tension (diluted 5 times) is ≤35mN / m.

[0017] Wherein, the chelated trace element mother solution is prepared by the following method; (1) Add 7 parts of EDTA-2Na to 70 parts of water, and stir to dissolve to obtain a chelating solution; (2) Weigh 8 parts of magnesium sulfate, 3 parts each of ferrous sulfate, copper sulfate and zinc sulfate, 2 parts each of sodium borate, manganese sulfate and calcium chloride, add them to the chelating solution prepared in step (1), stir and heat to 40-60°C, maintain for 2 hours, and obtain a mother solution of medium and trace elements.

[0018] The composite amino acid solution is prepared by dissolving 8 parts of arginine, 2 parts of leucine, 2 parts of glutamic acid and 3 parts of proline in 40% water; The urea solution is prepared by dissolving 10 parts of urea in 20 parts of water.

[0019] The preparation method of a lipopeptide amino acid water-soluble fertilizer of the present invention comprises the following steps: 18 parts of a composite amino acid solution, 18 parts of a medium and trace element mother solution, and 9 parts of a urea solution are mixed, and then 10 parts of a lipopeptide surfactant stock solution and 0.3 parts of pollen polysaccharide are added, the mixture is mixed evenly, and then 44.7 parts of water are added to make up the volume, and the mixture is stirred until dissolved and then filtered to remove water-insoluble impurities, so as to prepare the lipopeptide amino acid water-soluble fertilizer.

[0020] Example 2

[0021] The lipopeptide amino acid water-soluble fertilizer of the present invention is prepared from the following raw materials in parts by weight: 10 parts of lipopeptide surfactant, 16 parts of chelated trace element mother solution, 18 parts of composite amino acid solution, 5 parts of urea solution, 0.3 parts of pollen polysaccharide and 50.7 parts of water.

[0022] The preparation method of the lipopeptide surfactant stock solution is the same as that in Example 1.

[0023] Wherein, the chelated trace element mother solution is prepared by the following method; (1) Add 7 parts of EDTA-2Na to 70 parts of water and stir to dissolve to obtain a chelating solution; (2) Weigh 8 parts of magnesium sulfate, 3 parts each of ferrous sulfate, copper sulfate, zinc sulfate, and ammonium molybdate, and 2 parts each of sodium borate and manganese sulfate, add them to the chelating solution, stir and heat to 40-60°C, maintain for 2 hours, and obtain a mother solution of medium and trace elements.

[0024] The composite amino acid solution is prepared by dissolving 3 parts of glycine, 3 parts of alanine, 4 parts of histidine and 5 parts of aspartic acid in 40% water; The urea solution is prepared by dissolving 10 parts of urea in 20 parts of water.

[0025] The preparation method of a lipopeptide amino acid water-soluble fertilizer of the present invention comprises the following steps: 18 parts of a composite amino acid solution, 16 parts of a medium and trace element mother solution, and 5 parts of a urea solution are mixed, and then 10 parts of a lipopeptide surfactant stock solution and 0.3 parts of pollen polysaccharide are added, the mixture is mixed evenly, and then 50.7 parts of water are added to make up the volume, and the mixture is stirred until dissolved and then filtered to remove water-insoluble impurities, so as to prepare the lipopeptide amino acid water-soluble fertilizer.

[0026] Example 3

[0027] The lipopeptide amino acid water-soluble fertilizer of the present invention is prepared from the following raw materials in parts by weight: 10 parts of lipopeptide surfactant, 10 parts of chelated trace element mother solution, 18 parts of composite amino acid solution, 9 parts of urea solution, 0.4 parts of pollen polysaccharide and 52.6 parts of water.

[0028] The preparation method of the lipopeptide surfactant stock solution is the same as that in Example 1.

[0029] Wherein, the chelated trace element mother solution is prepared by the following method; (1) Add 7 parts of EDTA-2Na to 70 parts of water and stir to dissolve to obtain a chelating solution.

[0030] (2) Weigh 8 parts of magnesium sulfate, 3 parts each of ferrous sulfate, copper sulfate, zinc sulfate, and ammonium molybdate, and 2 parts each of sodium borate and manganese sulfate, add them to the chelating solution, stir and heat to 40-60°C, maintain for 2 hours, and obtain a mother solution of medium and trace elements.

[0031] The composite amino acid solution is prepared by dissolving 3 parts of lysine, 3 parts of serine, 3 parts of phenylalanine and 6 parts of tyrosine in 40% water; The urea solution is prepared by dissolving 10 parts of urea in 20 parts of water.

[0032] The preparation method of a lipopeptide amino acid water-soluble fertilizer of the present invention is the same as that of Example 1 or Example 2.

[0033] Example 4

[0034] The lipopeptide amino acid water-soluble fertilizer of the present invention is prepared from the following raw materials in parts by weight: 8 parts of lipopeptide surfactant, 15 parts of chelated trace element mother solution, 15 parts of composite amino acid solution, 6 parts of urea solution, 0.4 parts of pollen polysaccharide and 55.6 parts of water.

[0035] The preparation method of the lipopeptide surfactant stock solution is the same as that in Example 1.

[0036] Wherein, the chelated trace element mother solution is prepared by the following method; (1) Add 7 parts of EDTA-2Na to 70 parts of water and stir to dissolve to obtain a chelating solution.

[0037] (2) Weigh 6 parts of magnesium sulfate, 4 parts of ferrous sulfate and zinc sulfate, 2 parts of sodium borate and manganese sulfate, add them to the chelating solution, stir and heat to 40-60°C, maintain for 2 hours, and obtain a mother solution of medium and trace elements.

[0038] The composite amino acid solution is prepared by dissolving 5 parts of glutamic acid, 5 parts of proline and 5 parts of glycine in 40 parts of water; The urea solution is prepared by dissolving 10 parts of urea in 20 parts of water.

[0039] The preparation method of a lipopeptide amino acid water-soluble fertilizer of the present invention is the same as that of Example 1 or Example 2.

[0040] The lipopeptide stock solution in the lipopeptide amino acid water-soluble fertilizer of Example 1 was replaced with water, and the contact angle and wetting area comparison test was performed on the control amino acid water-soluble fertilizer and the lipopeptide amino acid water-soluble fertilizer of Example 1, Example 2, Example 3, and Example 4. The specific experimental method and results are as follows: spray fresh corn leaves with water, let them dry naturally, cut the leaves at the same part and fix them flatly on a glass slide, and use a contact angle meter HS-150A to detect the contact angle between the lipopeptide amino acid water-soluble fertilizer droplets and the control amino acid water-soluble fertilizer droplets and the leaves. Use a microinjector to take 1uL of lipopeptide amino acid water-soluble fertilizer droplets and control amino acid water-soluble fertilizer, respectively, and slowly drip them on the leaves at the same height, and use a microscope to observe the wetting area of ​​the solution on the leaves according to the ruler;

[0041] As can be seen from Table 1, the addition of lipopeptide surfactant in the present invention can reduce the contact angle between the fertilizer droplet and the leaf surface and increase the leaf wetting area.

[0042] The lipopeptide stock solution in the lipopeptide amino acid water-soluble fertilizer of Example 1 was replaced with water, and pollen polysaccharide was not added to obtain the "amino acid water-soluble fertilizer without lipopeptide and pollen polysaccharide control", and the lipopeptide amino acid water-soluble fertilizer of Example 1 was not added with pollen polysaccharide to obtain the "amino acid water-soluble fertilizer with only lipopeptide addition", and the lipopeptide stock solution in the lipopeptide amino acid water-soluble fertilizer of Example 1 was replaced with water to obtain the "amino acid water-soluble fertilizer with only pollen polysaccharide addition", and the above three groups of controls were tested for fertilizer efficiency improvement together with the lipopeptide amino acid water-soluble fertilizers of Examples 1, 2, 3, and 4. The experiment adopted the method of potted Chinese cabbage, and the specific experimental method and results were as follows: Weigh an equal amount of flower soil and put it into a flowerpot with a diameter of 15 cm, without applying base fertilizer, and sow 4 Chinese cabbage seeds on the soil surface, cover with thin soil, thin out after germination, and retain the best growing one. The same amount of water was then applied on the same day. Four kinds of amino acid water-soluble fertilizers were sprayed 10 days after seed germination, wherein the lipopeptide amino acid water-soluble fertilizers of Examples 1, 2, 3, and 4 were 0.8 times the amount of the control lipopeptide amino acid water-soluble fertilizer, and the second spraying was performed 15 days apart. The dry weight of the whole plant was detected after 35 days of cultivation;

[0043] As described in Table 2, by comparing the test results of "amino acid water-soluble fertilizer without lipopeptide and pollen polysaccharide control" and "amino acid water-soluble fertilizer with only lipopeptide added", it can be found that adding lipopeptide can improve the fertilizer efficiency of water-soluble fertilizer. By comparing the test results of "amino acid water-soluble fertilizer without lipopeptide and pollen polysaccharide control" and "amino acid water-soluble fertilizer with only pollen polysaccharide added", it can be found that adding pollen polysaccharide has a promoting effect on the growth of pakchoi and increases dry matter accumulation. By comparing the test results of 4 examples with "amino acid water-soluble fertilizer without lipopeptide and pollen polysaccharide control", it can be found that adding pollen polysaccharide and lipopeptide at the same time can effectively improve the fertilizer efficiency of water-soluble fertilizer and promote the growth of pakchoi, and the effect of joint addition is more obvious than that of single addition.

[0044] The lipopeptide stock solution in the lipopeptide amino acid water-soluble fertilizer of Example 1 was replaced with water to obtain a control lipopeptide amino acid water-soluble fertilizer and the lipopeptide amino acid water-soluble fertilizers of Examples 1, 2, and 3 for fertilizer efficiency improvement tests. The test adopted the method of potted Chinese cabbage, and the specific experimental method and results are as follows: Weigh an equal amount of flower soil and put it into a flowerpot with a diameter of 15 cm. No base fertilizer is applied. Four Chinese cabbage seeds are sown on the soil surface, covered with a thin layer of soil, and thinned out after germination, retaining the one with the best growth. Subsequently, watering was carried out in equal amounts on the same day. Four kinds of amino acid water-soluble fertilizers were sprayed 10 days after the seeds germinated, among which the lipopeptide amino acid water-soluble fertilizers of Examples 1, 2, 3, and 4 were 0.8 times the amount of the control lipopeptide amino acid water-soluble fertilizer, and the second spraying was carried out at an interval of 15 days. The dry weight of the whole plant was detected after 35 days of cultivation;

[0045] As can be seen from Table 3, the amino acid water-soluble fertilizer prepared by adding lipopeptide surfactant in the present invention still has a promoting effect on the growth of pakchoi after reducing the fertilizer dosage by 20%, indicating that the lipopeptide amino acid water-soluble fertilizer has the effect of saving fertilizer and increasing efficiency.

[0046] The lipopeptide amino acid water-soluble fertilizers of Examples 1, 2, 3, and 4 were used in a corn field test together with a common amino acid water-soluble fertilizer on the market (the amino acid water-soluble fertilizer Shi Kefeng was used in this test).

[0047] The experiment was divided into 5 plots, each plot had an area of ​​1.2 mu, and the same variety and amount of base fertilizer were used. Four kinds of water-soluble fertilizers were sprayed at the small trumpet stage and the large trumpet stage of corn, respectively, among which the lipopeptide amino acid water-soluble fertilizer of Examples 1, 2, 3, and 4 was 0.8 times the amount of the control amino acid water-soluble fertilizer, and samples were taken to measure yield after maturity.

[0048] As can be seen from Table 4, the lipopeptide amino acid water-soluble fertilizer of the present invention increases corn yield by increasing the number of corn cobs and 100-grain weight. When the dosage is reduced by 20%, the yield increase is 13.68%-20.06% compared with a common amino acid water-soluble fertilizer on the market used as a control, and the product has obvious fertilizer effect.

Claims

1. A lipopeptide amino acid water-soluble fertilizer, characterized in that: The invention is prepared from the following components by weight: 5-12 parts of lipopeptide surfactant stock solution, 10-20 parts of chelated trace element mother solution, 15-20 parts of composite amino acid solution, 5-10 parts of urea solution, 0.1-0.5 parts of pollen polysaccharide and 37.5-64.9 parts of water.

2. A lipopeptide amino acid water-soluble fertilizer as described in claim 1, characterized in that: The composite amino acid is any one or more of arginine, leucine, glutamic acid, proline, glycine, alanine, histidine, aspartic acid, lysine, serine, phenylalanine and tyrosine in any proportion.

3. A lipopeptide amino acid water-soluble fertilizer as described in claim 1, characterized in that: The content of the lipopeptide surfactant in the lipopeptide surfactant stock solution is ≥1.5 g / L.

4. A lipopeptide amino acid water-soluble fertilizer as claimed in claim 1, characterized in that: The chelated trace element mother solution is prepared by the following method; (1) Add 4-7 parts of EDTA-2Na to 63-74 parts of water, stir and dissolve to obtain a chelating solution; (2) Add one or more of magnesium sulfate, magnesium chloride, ferrous sulfate, ammonium molybdate, copper sulfate, zinc sulfate, sodium borate, manganese sulfate, and calcium chloride to the chelating solution, stir and heat to 40-60° C., maintain for 2 hours, and obtain a mother solution of medium and trace elements.

5. A lipopeptide amino acid water-soluble fertilizer as claimed in claim 4, characterized in that The added amounts of the magnesium sulfate, magnesium chloride, ferrous sulfate, ammonium molybdate, copper sulfate, zinc sulfate, sodium borate, manganese sulfate and calcium chloride are 2 to 10 parts respectively.

6. A method for preparing a lipopeptide amino acid water-soluble fertilizer as described in claim 1, characterized in that: The steps include: (1) Weighing the composite amino acid according to the mass fraction, dissolving a total of 15 parts of the composite amino acid in 40 parts of water to obtain a composite amino acid solution; (2) Weighing urea by mass, dissolving 10 parts of the urea in 20 parts of water to obtain a urea solution; (3) After mixing the composite amino acid solution, the mother solution of trace elements and the urea solution, the lipopeptide surfactant solution and the pollen polysaccharide are added, the mixture is evenly mixed and then water is added to make up the volume. After stirring until dissolved, the non-water-soluble impurities are filtered out to obtain the lipopeptide amino acid water-soluble fertilizer.

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