Sample set sampler and drug sensitive test method

Abstract

The invention discloses a sample set sampler and a drug sensitivity test method, and relates to the technical field of drug sensitivity detection. The sample set sampler comprises a collection unit; the collection unit comprises a collector and a sampling needle arranged at the end of the collector, and the sampling needle is connected with the culture unit; a culture unit; the culture unit comprises a culture chamber and an injection chamber, a partition plate is arranged between the culture chamber and the injection chamber, an injection needle is arranged at the bottom of the injection chamber, and the injection needle is connected with the detection unit; a detection unit; the detection unit comprises a plate, a plate cover is arranged above the plate, and a drug sensitivity paper sheet for drug sensitivity detection is arranged in the plate. According to the sample set sampler and the drug sensitive test method, equipment is simple and low in cost, meanwhile, the number of times of suction and transfer of sample liquid is reduced, pollution is reduced, and the size of an inhibition zone can be observed and measured more visually due to the fact that the measuring ring and the scale marks are arranged on the periphery of the drug sensitive paper piece.

Description

Technical Field

[0001] The invention relates to the technical field of drug sensitivity detection, and in particular to a sample collection sampler and a drug sensitivity test method. Background Art

[0002] In the animal husbandry industry, especially with the increasing degree of intensive farming, the economic losses caused by various bacterial diseases to the farming industry have received much attention, so the use of antibiotics to prevent and treat bacterial diseases has become the norm. However, the drug-resistant strains produced by the large-scale use of antibiotics may transfer drug-resistant genes from animals to humans, posing a potential threat to human health. With the improvement of people's awareness of food safety and the increasing attention of the government to food safety, the use of antibiotics in farms has become more and more standardized. The selection of sensitive drugs for pathogens can achieve precise control of animal diseases, reduce the cost of drug treatment, and reduce drug residues in animal food.

[0003] The veterinarian of the farm determines the type of animal disease based on the clinical and pathological autopsy of the animal. For bacterial diseases, sensitive drugs can be screened based on drug sensitivity tests, so as to scientifically use drugs to treat and prevent bacterial diseases. The use of drug sensitivity tests can not only detect the sensitivity of pathogens to various antimicrobial drugs relatively quickly and effectively, and timely understand the drug resistance of pathogenic microorganisms in the farm, but more importantly, it can guide clinical scientific and rational drug use. Existing drug sensitivity tests are often performed using drug sensitivity test kits, but current drug sensitivity kits generally have the disadvantages of being expensive, having outdated and single drug types, and insufficient test operability. Therefore, the present application provides a sample set sampler and drug sensitivity test method with simple equipment and low price. Summary of the invention

[0004] The purpose of the present invention is to provide a sample set sampler and a drug sensitivity test method, which has simple equipment and low price, and reduces the number of times of aspiration and transfer of sample liquid, reduces pollution, and measurement circles and scale lines are arranged around the drug sensitivity paper sheet, so that the size of the inhibition zone can be observed and measured more intuitively.

[0005] To achieve the above object, the present invention provides a sample collection sampler, comprising

[0006] The collecting unit includes a collector and a sampling needle disposed at the end of the collector, and the sampling needle is connected to the culture unit;

[0007] The culture unit comprises a culture chamber and an injection chamber, a partition is provided between the culture chamber and the injection chamber, an injection needle is provided at the bottom of the injection chamber, and the injection needle is connected to the detection unit;

[0008] Detection unit: The detection unit includes a plate, a plate cover is arranged above the plate, and a drug-sensitive paper sheet for drug sensitivity detection is arranged inside the plate.

[0009] Furthermore, the collector is a syringe for collecting samples.

[0010] Furthermore, a rubber stopper is provided above the culture chamber, a sampling needle is inserted into the rubber stopper and connected to the culture chamber, and liquid culture medium is pre-installed inside the culture chamber.

[0011] Furthermore, a filter is provided at the bottom of the partition, the filter is provided on both sides of the partition, a through groove is provided inside the partition, an adjustment rod is provided inside the through groove, a lifting plate is provided at the bottom of the adjustment rod, and the lifting plate is provided between the filter screens on both sides of the partition.

[0012] Furthermore, a piston rod is provided inside the injection chamber, the upper end of the piston rod passes through the top of the injection chamber, and a piston head is provided below the piston rod.

[0013] Furthermore, the bottom of the injection chamber is arranged as a conical mouth, a sealing film is arranged inside the conical mouth for sealing, a fixing plate for fixing the injection needle is arranged outside the conical mouth, a protrusion is arranged inside the fixing plate, a mounting plate is arranged at the upper end of the injection needle, a groove is arranged on the side of the mounting plate, the groove is adapted to the protrusion, the mounting plate is clamped between the conical mouth and the fixing plate, and the injection needle is connected to the conical mouth through the mounting plate.

[0014] Furthermore, the injection needle is configured to be hollow, with a sharp corner at the top of the injection needle, an upper through hole at the side of the sharp corner, a pointed tip at the bottom of the injection needle, a lower through hole at the tip, and the upper through hole is connected to the lower through hole.

[0015] Furthermore, the petri dish and the petri dish cover are made of disposable plastic material.

[0016] Furthermore, the drug-sensitive paper sheet is arranged on the surface of the plastic film, and there are several drug-sensitive paper sheets. Several measuring circles are arranged around each drug-sensitive paper sheet, and scale lines are arranged on the measuring circles.

[0017] The present invention also provides a method for drug sensitivity testing of a sample collection sampler, wherein the method uses the sample collection sampler to perform a test, and comprises the following steps:

[0018] Step 1, install the sampling needle on the end of the collector, use the collection unit to collect samples, after the sample collection is completed, insert the sampling needle into the rubber stopper above the culture chamber, and inject the sample into the culture chamber pre-filled with liquid culture medium;

[0019] Step 2, placing the culture chamber under suitable conditions for culture;

[0020] Step 3: After the culture is completed, pull the adjustment rod upwards, the lifting plate rises, and the sample liquid passes through the filter into the injection chamber, and then press the adjustment rod downwards, the lifting plate descends, and the channel is closed;

[0021] Step 4, the mounting plate is clamped between the conical port and the fixing plate, the injection needle is connected to the conical port through the mounting plate, the sharp corner of the upper end of the injection needle pierces the sealing film, and then the sample liquid is injected into the plate pre-filled with solid culture medium through the piston rod and the piston head, and then coated;

[0022] Step 5, placing the drug-sensitive paper on the surface of the plate so that the drug-sensitive paper is in contact with the solid culture medium, and then placing the plate under appropriate conditions for culture;

[0023] Step 6: After the incubation is completed, observe, measure and record the size of the inhibition zone through the measuring circle and the scale line. The advantages and positive effects of the sample collection sampler and drug sensitivity test method described in the present invention are:

[0024] 1. The sample collection sampler in the present invention is simple in equipment, low in price, and made of transparent material as a whole, which is convenient for observation. At the same time, after the cultivation is completed, no other tools are needed to absorb and transfer the sample liquid, which reduces the number of times the sample liquid is absorbed and transferred, and reduces pollution.

[0025] 2. The sample set sampler in the present invention is provided with measuring circles and scale lines around the drug-sensitive paper sheet, so that the size of the inhibition zone can be observed and measured more intuitively.

[0026] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic diagram of the overall structure of the sample set sampler in an embodiment of the present invention;

[0028] Figure 2 Schematic diagram of the partition structure of the sample collection sampler in an embodiment of the present invention;

[0029] Figure 3 for Figure 1 Enlarged view of point A in the middle;

[0030] Figure 4 Schematic diagram of the injection needle structure of the sample collection sampler in the embodiment of the present invention Figure 1 ;

[0031] Figure 5 Schematic diagram of the injection needle structure of the sample collection sampler in the embodiment of the present invention Figure 2 ;

[0032] Figure 6 Schematic diagram of a detection unit of a sample set sampler in an embodiment of the present invention;

[0033] Figure 7 Schematic diagram of the drug-sensitive paper sheet of the sample set sampler in an embodiment of the present invention.

[0034] Reference numerals

[0035] 1. Collector; 2. Sampling needle; 3. Rubber stopper; 4. Culture chamber; 5. Adjustment rod; 6. Partition; 7. Lifting plate; 8. Piston rod; 9. Piston head; 10. Injection chamber; 11. Conical mouth; 12. Through groove; 13. Filter; 14. Protrusion; 15. Fixing plate; 16. Sharp corner; 17. Upper through hole; 18. Mounting plate; 19. Groove; 20. Injection needle; 21. Lower through hole; 22. Petri dish; 23. Petri dish cover; 24. Drug-sensitive paper; 25. Plastic film; 26. Scale line; 27. Measuring circle. DETAILED DESCRIPTION

[0036] The technical solution of the present invention is further described below through the accompanying drawings and embodiments.

[0037] Unless otherwise defined, the technical terms or scientific terms used in the present invention should be understood by people with ordinary skills in the field to which the present invention belongs. The words "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0038] Example 1

[0039] like Figure 1 As shown, a sample collection sampler includes a collection unit; the collection unit includes a collector 1 and a sampling needle 2 arranged at the end of the collector 1, and the sampling needle 2 is connected to the culture unit. Culture unit; the culture unit includes a culture chamber 4 and an injection chamber 10, a partition 6 is arranged between the culture chamber 4 and the injection chamber 10, an injection needle 20 is arranged at the bottom of the injection chamber 10, and the injection needle 20 is connected to the detection unit. Detection unit; the detection unit includes a plate 22, a plate cover 23 is arranged above the plate 22, and a drug-sensitive paper sheet 24 for drug sensitivity detection is arranged inside the plate 22. The entire sample collection sampler is selected from transparent materials according to needs, which is convenient for observation and reduces costs. The collector 1 is a syringe for collecting samples. The syringe can select an existing syringe according to needs.

[0040] like Figure 1As shown, a rubber stopper 3 is arranged above the culture chamber 4, and a sampling needle 2 is inserted into the rubber stopper 3 and connected to the culture chamber 4, and a liquid culture medium is pre-installed inside the culture chamber 4. The collected sample is passed into the culture chamber 4 through the sampling needle 2.

[0041] like Figure 2 As shown, a filter screen 13 is provided at the bottom of the partition 6, and the filter screen 13 is provided on both sides of the partition 6. A through slot 12 is provided inside the partition 6, and an adjusting rod 5 is provided inside the through slot 12. A lifting plate 7 is provided at the bottom of the adjusting rod 5, and the lifting plate 7 is provided between the filter screens 13 on both sides of the partition 6. When the adjusting rod 5 is pulled upward, the lifting plate 7 rises, and the sample liquid enters the injection chamber 10 from the culture chamber 4. When the adjusting rod 5 is pressed downward, the lifting plate 7 descends, and the channel is closed.

[0042] like Figure 1 and Figure 3 As shown, a piston rod 8 is provided inside the injection chamber 10, and the upper end of the piston rod 8 passes through the top of the injection chamber 10, and a piston head 9 is provided below the piston rod 8. The bottom of the injection chamber 10 is provided with a conical mouth 11, and a sealing film for sealing is provided inside the conical mouth 11. A fixing plate 15 for fixing the injection needle 20 is provided on the outside of the conical mouth 11, and a protrusion 14 is provided on the inner side of the fixing plate 15. A mounting plate 18 is provided at the upper end of the injection needle 20, and a groove 19 is provided on the side of the mounting plate 18, and the groove 19 is adapted to the protrusion 14. The mounting plate 18 is clamped between the conical mouth 11 and the fixing plate 15, and the injection needle 20 is connected to the conical mouth 11 through the mounting plate 18.

[0043] like Figure 4 and Figure 5 As shown, the injection needle 20 is configured to be hollow, with a sharp corner 16 disposed on the top of the injection needle 20, an upper through hole 17 disposed on the side of the sharp corner 16, and a tip disposed at the bottom of the injection needle 20, with a lower through hole 21 disposed at the tip, and the upper through hole 17 is connected to the lower through hole 21. The sharp corner 16 is used to pierce the sealing film at the conical opening 11, so that the sample liquid in the injection chamber 10 flows out through the injection needle 20. The diameter of the bottom of the sharp corner 16 is exactly the same as the diameter at the conical opening 11.

[0044] like Figure 6 As shown, the petri dish 22 and the petri dish cover 23 may be made of disposable plastic material.

[0045] like Figure 7 As shown, the drug-sensitive paper sheet 24 is arranged on the surface of the plastic film 25, and there are several drug-sensitive paper sheets 24, and several measuring circles 27 are arranged around each drug-sensitive paper sheet 24, and scale lines 26 are arranged on the measuring circles 27. The size of the antibacterial zone can be intuitively observed, measured and recorded through the measuring circles 27 and the scale lines 26.

[0046] Example 2

[0047] A method for drug sensitivity testing of a sample collection sampler, using the sample collection sampler to perform a test, comprising the following steps:

[0048] Step 1, install the sampling needle 2 at the end of the collector 1, use the collection unit to collect samples, after the sample collection is completed, insert the sampling needle 2 into the rubber stopper 3 above the culture chamber 4, and inject the sample into the culture chamber 4 pre-filled with liquid culture medium;

[0049] Step 2, placing the culture chamber 4 under suitable conditions for culture;

[0050] Step 3, after the culture is completed, the adjusting rod 5 is pulled upward, the lifting plate 7 rises, and the sample liquid enters the injection chamber 10 through the filter 13, and then the adjusting rod 5 is pressed downward, the lifting plate 7 descends, and the channel is closed;

[0051] Step 4, the mounting plate 18 is clamped between the tapered opening 11 and the fixing plate 15, the injection needle 20 is connected to the tapered opening 11 through the mounting plate 18, the sharp corner 16 at the upper end of the injection needle 20 pierces the sealing film, and then the sample liquid is injected into the plate 22 pre-filled with the solid culture medium through the piston rod 8 and the piston head 9, and the coating is performed;

[0052] Step 5, placing the drug-sensitive paper sheet 24 on the surface of the plate 22, so that the drug-sensitive paper sheet 24 is in contact with the solid culture medium, and then placing the plate 22 under appropriate conditions for culture;

[0053] Step 6: After the incubation is completed, the size of the inhibition zone is observed, measured and recorded through the measuring circle 27 and the scale line 26.

[0054] The sample collection sampler in the present invention is simple in equipment, low in price, and made of transparent material as a whole, which is convenient for observation. After the cultivation is completed, no other tools are needed to absorb and transfer the sample liquid, which reduces the number of times the sample liquid is absorbed and transferred and reduces pollution.

[0055] Therefore, the present invention adopts the above-mentioned sample set sampler and drug sensitivity test method, which has simple equipment and low price, and at the same time reduces the number of times the sample liquid is absorbed and transferred, reduces pollution, and measurement circles and scale lines are arranged around the drug-sensitive paper sheet, which can more intuitively observe and measure the size of the inhibition zone.

[0056] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A sample collection sampler, characterized in that: include The collecting unit includes a collector and a sampling needle disposed at the end of the collector, and the sampling needle is connected to the culture unit; Training unit; The culture unit includes a culture chamber and an injection chamber, a partition is provided between the culture chamber and the injection chamber, an injection needle is provided at the bottom of the injection chamber, and the injection needle is connected to the detection unit; Detection unit: The detection unit includes a plate, a plate cover is arranged above the plate, and a drug-sensitive paper sheet for drug sensitivity detection is arranged inside the plate.

2. A sample collection sampler according to claim 1, characterized in that: The collector is a syringe used for collecting samples.

3. A sample collection sampler according to claim 1, characterized in that: A rubber plug is arranged above the culture chamber, a sampling needle is inserted into the rubber plug and connected to the culture chamber, and a liquid culture medium is pre-installed in the culture chamber.

4. A sample collection sampler according to claim 1, characterized in that: A filter screen is arranged at the bottom of the partition, the filter screens are arranged on both sides of the partition, a through slot is arranged inside the partition, an adjusting rod is arranged inside the through slot, a lifting plate is arranged at the bottom of the adjusting rod, and the lifting plate is arranged between the filter screens on both sides of the partition.

5. A sample collection sampler according to claim 1, characterized in that: A piston rod is arranged inside the injection chamber, the upper end of the piston rod passes through the top of the injection chamber, and a piston head is arranged below the piston rod.

6. A sample collection sampler according to claim 1, characterized in that: The bottom of the injection chamber is arranged as a conical mouth, a sealing film is arranged inside the conical mouth, a fixing plate for fixing the injection needle is arranged outside the conical mouth, a protrusion is arranged inside the fixing plate, a mounting plate is arranged at the upper end of the injection needle, a groove is arranged on the side of the mounting plate, the groove is matched with the protrusion, the mounting plate is clamped between the conical mouth and the fixing plate, and the injection needle is connected to the conical mouth through the mounting plate.

7. A sample collection sampler according to claim 1, characterized in that: The injection needle is arranged to be hollow, with a sharp corner arranged on the top of the injection needle, an upper through hole arranged on the side of the sharp corner, a pointed tip arranged on the bottom of the injection needle, a lower through hole arranged on the tip, and the upper through hole is connected with the lower through hole.

8. A sample collection sampler according to claim 1, characterized in that: The petri dish and the petri dish cover are made of disposable plastic material.

9. A sample collection sampler according to claim 1, characterized in that: The drug-sensitive paper sheet is arranged on the surface of the plastic film, and a plurality of drug-sensitive paper sheets are arranged. A plurality of measuring circles are arranged around each drug-sensitive paper sheet, and scale lines are arranged on the measuring circles.

10. A method for drug sensitivity testing of a sample collection sampler, characterized in that: The test is carried out using the sample set sampler according to any one of claims 1 to 9, comprising the following steps: Step 1, install the sampling needle on the end of the collector, use the collection unit to collect samples, after the sample collection is completed, insert the sampling needle into the rubber stopper above the culture chamber, and inject the sample into the culture chamber pre-filled with liquid culture medium; Step 2, placing the culture chamber under suitable conditions for culture; Step 3: After the culture is completed, pull the adjustment rod upwards, the lifting plate rises, and the sample liquid passes through the filter into the injection chamber, and then press the adjustment rod downwards, the lifting plate descends, and the channel is closed; Step 4, the mounting plate is clamped between the conical port and the fixing plate, the injection needle is connected to the conical port through the mounting plate, the sharp corner of the upper end of the injection needle pierces the sealing film, and then the sample liquid is injected into the plate pre-filled with solid culture medium through the piston rod and the piston head, and then coated; Step 5, placing the drug-sensitive paper on the surface of the plate so that the drug-sensitive paper is in contact with the solid culture medium, and then placing the plate under appropriate conditions for culture; Step 6: After the incubation, observe, measure and record the size of the inhibition zone through the measuring circle and scale line.

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