Microbial culture medium anaerobic control device and method, electronic equipment and storage medium

Abstract

The invention provides a microbial culture medium anaerobic control device and method, electronic equipment and a storage medium, and relates to the technical field of microbial culture, the device comprises a gas storage tank, a deoxidation device and an anaerobic culture device which are connected; the anaerobic culture device comprises: an anaerobic culture bottle having a culture chamber and a first opening; the bottle cap is used for blocking the first opening; the gas washing pipe assembly comprises a gas inlet pipe of which the first end is connected with the deoxidation device and the second end extends into the culture cavity; the porous structure is connected with the second end and is arranged in the culture cavity; the gas washing pipe sleeves the gas inlet pipe, at least one part of the gas washing pipe extends into the culture cavity, and a gas outlet is formed in the side wall of the gas washing pipe; the air outlet pipe is connected with the air outlet. According to the technical scheme, the deoxidizing device and the anaerobic culture device are separately arranged, the deoxidizing device is arranged between the inert gas storage tank and the anaerobic culture device, and before gas enters the anaerobic culture bottle, oxygen in the gas can be removed by a deoxidizing agent, so that the influence of the deoxidizing agent and the oxygen on the growth of microorganisms is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial culture, and in particular to an anaerobic control device, method, electronic equipment and storage medium for a microbial culture medium. Background Art

[0002] For the cultivation of strictly anaerobic microorganisms, it is necessary to eliminate oxygen in the culture system. Even the presence of trace amounts of oxygen will cause irreversible inactivation or even death of the microorganisms in the culture system. Therefore, oxygen control technology is critical for the cultivation of strictly anaerobic microorganisms.

[0003] In the related art, an inert gas is directly introduced into the culture bottle to replace the oxygen in the culture system to create anaerobic conditions. However, there is usually some oxygen in the inert gas, which will inhibit the growth of anaerobic microorganisms. Alternatively, a deoxidizer is directly placed in the culture bottle to consume the oxygen in the culture system to create anaerobic conditions. However, the residual deoxidizer will chemically react with the anaerobic microorganisms, thereby inhibiting the growth of anaerobic microorganisms. Summary of the invention

[0004] In order to solve or improve the technical problem that oxygen or residual deoxidizer may affect the growth of anaerobic microorganisms, an object of the present invention is to provide an anaerobic control device for microbial culture medium.

[0005] Another object of the present invention is to provide a control method.

[0006] Another object of the present invention is to provide an electronic device.

[0007] Another object of the present invention is to provide a readable storage medium.

[0008] To achieve the above-mentioned object, the first aspect of the present invention provides an anaerobic control device for a microbial culture medium, comprising a connected gas storage tank, a deoxygenation device and an anaerobic culture device.

[0009] Among them, the gas storage tank is used to store gas, and the gas is nitrogen, hydrogen or inert gas; the deoxidation device is used to place the deoxidizer.

[0010] The anaerobic culture device comprises an anaerobic culture bottle, a bottle cover and a gas washing pipe assembly.

[0011] The anaerobic culture bottle has a culture cavity and a first opening, and the first opening is connected to the culture cavity. The bottle cap is detachably connected to the anaerobic culture bottle. When the bottle cap and the anaerobic culture bottle are in a connected state, the bottle cap is used to block the first opening.

[0012] The air washing pipe assembly comprises an air inlet pipe, a porous structure, an air washing pipe and an air outlet pipe.

[0013] The air inlet pipe has a first end and a second end. The first end is used to connect the deoxygenation device through an air inlet hose. At least a portion of the air inlet pipe is passed through the bottle cap, and the second end can extend into the culture chamber. The porous structure is connected to the second end, and the porous structure is arranged in the culture chamber. One end of the wash pipe is provided with a blocking structure. The wash pipe is sleeved on the air inlet pipe. At least a portion of the wash pipe is passed through the bottle cap, and the other end of the wash pipe can extend into the culture chamber. An air outlet cavity is formed between the inner tube wall of the wash pipe and the outer tube wall of the air inlet pipe. An air outlet is formed on the side wall of the wash pipe, and the air outlet is connected to the air outlet cavity. One end of the outlet pipe is connected to the air outlet.

[0014] The air intake hose comprises a first pipe section and a second pipe section. One end of the first pipe section is connected to the deoxygenation device, and one end of the second pipe section is connected to the first end.

[0015] The microbial culture medium anaerobic control device also includes a connecting valve, a first control valve, a second control valve and a third control valve.

[0016] The connecting valve is used to connect the first pipe section and the second pipe section. The first control valve is arranged on the first pipe section, and the first control valve is used to control the on-off of the first pipe section. The second control valve is arranged on the second pipe section, and the second control valve is used to control the on-off of the second pipe section. The third control valve is arranged on the air outlet pipe, and the third control valve is used to control the on-off of the air outlet pipe.

[0017] The present invention aims to provide an anaerobic control device for a microbial culture medium. In the first aspect, a deoxidizing device and an anaerobic culture device are separately arranged, so that the oxygen in the culture system can be consumed by a deoxidizer, and the residual deoxidizer can be prevented from chemically reacting with the culture medium or anaerobic microorganisms, which is beneficial to ensuring the growth rate of anaerobic microorganisms and the stability of the experiment; in the second aspect, a gas storage tank and a deoxidizing device are arranged at the same time, and the deoxidizing device is arranged between the gas storage tank and the anaerobic culture device. Before the gas (nitrogen, hydrogen or inert gas) enters the anaerobic culture bottle of the anaerobic culture device, the deoxidizing agent can remove the residual oxygen in the gas, which is beneficial to ensuring that nitrogen, hydrogen or inert gas replaces the oxygen in the culture system to create anaerobic conditions; in the third aspect, the gas washing pipe is sleeved on the air inlet pipe. This design method is beneficial to optimizing the spatial layout and making the overall structure more compact.

[0018] It should be emphasized that the air wash pipe assembly is composed of an air inlet pipe, a porous structure, an air wash pipe and an air outlet pipe. By optimizing the spatial layout of the air wash pipe assembly, it is helpful to make the overall structure more compact.

[0019] In addition, the above technical solution provided by the present invention may also have the following additional technical features: In some technical solutions, the porous structure may include a first shell having a gas-uniform cavity therein, the first shell being connected to the second end, the air inlet pipe being connected to the gas-uniform cavity, and a plurality of gas-uniform holes being connected to the gas-uniform cavity.

[0020] In this technical solution, by limiting the aperture of the uniform air holes, smaller bubbles can be produced, increasing the contact area between the gas and the culture medium, which is beneficial for oxygen to diffuse quickly from the culture medium into the bubbles and be carried out of the culture system; at the same time, the evenly distributed uniform air holes can ensure the uniform dispersion of the gas in the culture medium, avoiding local uneven gas flow that affects the deoxygenation effect or the stability of the microbial growth environment.

[0021] In some technical solutions, optionally, at least a portion of the air intake pipe is passed through the first shell, and the second end is arranged in the air uniforming cavity, and the air intake pipe is sealed and connected to the first shell.

[0022] In this technical solution, by setting the air inlet pipe and the first shell as a sealed connection, it is ensured that the gas passes through the second end of the air inlet pipe, the gas uniformity cavity, the gas uniformity hole and the culture cavity in sequence, which is conducive to full contact between the gas and the culture medium.

[0023] In some technical solutions, optionally, the bottle cap includes a first part and a second part. The second part is an annular structure, and the second part is arranged around the first part. The second part and the first part surround a mounting groove. At least a part of the anaerobic culture bottle is arranged in the mounting groove, the first part is used to block the first opening, and a notch is arranged on the first part, and an end of the gas washing pipe away from the blocking structure can extend into the culture chamber through the notch.

[0024] In this technical solution, when the bottle cap and the anaerobic culture bottle are in a connected state, the bottle cap and the anaerobic culture bottle are in a sealed connection. By ensuring the sealing of the bottle cap to the first opening, it is effectively prevented that outside air enters the anaerobic culture bottle and causes contamination.

[0025] In some technical solutions, optionally, the anaerobic culture device further comprises a sealing pressure ring. The sealing pressure ring is arranged in the installation groove, and the sealing pressure ring is arranged between the first opening and the first part.

[0026] In this technical solution, by providing a sealing pressure ring, when the bottle cap and the anaerobic culture bottle are in a connected state, the sealing performance of the bottle cap for the first opening can be further improved, effectively preventing external air from entering the anaerobic culture bottle and causing contamination.

[0027] In some technical solutions, the deoxidation device may include a deoxidation tube, a first end cap and a second end cap. The deoxidation tube is used to place a deoxidizer. The first end cap is provided at one end of the deoxidation tube, and the first end cap is used to connect to one end of the first pipe section; the second end cap is provided at the other end of the deoxidation tube, and the second end cap is used to connect to a gas storage tank.

[0028] In this technical solution, by setting up the deoxygenation device separately (the deoxygenation device and the anaerobic culture device are set separately), on the one hand, it can avoid the chemical reaction between the residual deoxygenator and the culture medium or anaerobic microorganisms, which is beneficial to ensure the growth rate of anaerobic microorganisms and the stability of the experiment; on the other hand, it is convenient for the experimenter to replace the deoxygenator in time.

[0029] In some technical solutions, optionally, the first end cap is detachably connected to the deoxidation tube; and / or the second end cap is detachably connected to the deoxidation tube.

[0030] In this technical solution, this design makes it convenient for the staff to disassemble and assemble the first end cap or the second end cap to open or close the deoxidation tube.

[0031] When the end cap (the first end cap or the second end cap) is separated from the deoxidation tube, the staff can replace the deoxidizer in time to ensure that oxygen in the gas is removed.

[0032] The second aspect of the present invention provides a control method for use in the microbial culture medium anaerobic control device in any of the above technical solutions. The control method comprises the following steps: The first step is to close the first control valve, the second control valve and the third control valve in the microbial culture medium anaerobic control device.

[0033] In the second step, when it is determined that the culture medium is in the anaerobic culture bottle of the anaerobic culture device of the microbial culture medium anaerobic control device, the first control valve and the second control valve are opened to allow the gas in the gas storage tank of the microbial culture medium anaerobic control device to enter the deoxygenation device and the anaerobic culture bottle of the microbial culture medium anaerobic control device in turn.

[0034] In a third step, the third control valve is opened after the first time period.

[0035] The fourth step is to close the first control valve, the second control valve and the third control valve after a second time period, wherein the second time period is greater than the first time period.

[0036] In the technical solution defined in the present invention, the gas in the gas storage tank enters the deoxygenation device and the anaerobic culture device in turn. In this design, on the one hand, the deoxygenation device and the anaerobic culture device are set separately, which can not only consume the oxygen in the culture system through the deoxygenator, but also avoid the residual deoxygenator from chemically reacting with the culture medium or anaerobic microorganisms, which is beneficial to ensure the growth rate of anaerobic microorganisms and the stability of the experiment; on the other hand, the gas storage tank and the deoxygenation device are set at the same time, and the deoxygenation device is set between the gas storage tank and the anaerobic culture device. Before the gas (nitrogen, hydrogen or inert gas) enters the anaerobic culture bottle of the anaerobic culture device, the deoxygenation agent can remove the residual oxygen in the gas, which is beneficial to ensure that nitrogen, hydrogen or inert gas replaces the oxygen in the culture system to create anaerobic conditions.

[0037] The third aspect of the present invention provides an electronic device, the electronic device comprising a memory and a processor. The memory stores a program or instruction that can be run on the processor, and the processor implements the steps of the control method in the above technical solution when executing the program or instruction. The electronic device has the beneficial effects of the above technical solution, which will not be repeated here.

[0038] In a fourth aspect, the present invention provides a readable storage medium, which stores a program or instruction, and when the program or instruction is executed by a processor, the steps of the control method in the above technical solution are implemented. The readable storage medium has the beneficial effects of the above technical solution, which will not be repeated here.

[0039] Additional aspects and advantages of the technical solutions of the present invention will become apparent in the following description or will be understood through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 A schematic structural diagram of an anaerobic control device for a microbial culture medium according to an embodiment of the present invention is shown; Figure 2 Shows Figure 1 A partial enlarged schematic diagram of part A; Figure 3 A schematic structural diagram of an air scrubber pipe assembly according to an embodiment of the present invention is shown; Figure 4 Shows Figure 1 A partial enlarged schematic diagram of part B; Figure 5 Shows Figure 1 A partial enlarged schematic diagram of part C in the middle; Figure 6 A flow chart of a control method according to an embodiment of the present invention is shown; Figure 7 A structural block diagram of an electronic device according to an embodiment of the present invention is shown.

[0041] in, Figures 1 to 7 The corresponding relationship between the reference numerals and the component names is as follows: 100: anaerobic control device for microbial culture medium; 110: gas storage tank; 120: deoxygenation device; 121: deoxygenation tube; 122: first end cap; 123: second end cap; 124: deoxidizer; 130: anaerobic culture device; 131: anaerobic culture bottle; 1311: culture chamber; 1312: first opening; 132: bottle cap; 1321: first part; 1322: second part; 1323: mounting groove; 1324: notch; 133: air inlet pipe; 1331: first end; 1332: second end; 134: porous structure; 1341: first end A shell; 1342: an air-uniform hole; 1343: an air-uniform cavity; 135: an air-washing pipe; 1351: a blocking structure; 1352: an air outlet cavity; 1353: an air outlet; 136: an air outlet pipe; 137: a sealing ring; 140: an air intake hose; 141: a first pipe section; 142: a second pipe section; 150: a connecting valve; 151: a connecting sub-head; 152: a connecting female head; 161: a first control valve; 162: a second control valve; 163: a third control valve; 170: an air-washing pipe assembly; 300: an electronic device; 310: a memory; 320: a processor. DETAILED DESCRIPTION

[0042] In order to more clearly understand the above-mentioned purposes, features and advantages of the embodiments of the present invention, the embodiments of the present invention are further described in detail below in conjunction with the accompanying drawings and specific implementation methods. It should be noted that the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

[0043] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the embodiments of the present invention may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited to the specific embodiments disclosed below.

[0044] Refer to the following Figures 1 to 7 The present invention describes an anaerobic control device, method, electronic device and storage medium for a microbial culture medium according to some embodiments of the present invention.

[0045] It should be noted that Figure 1 , Figure 2 and Figure 4 The arrows in the figure indicate the direction of gas flow.

[0046] In one embodiment according to the present invention, Figure 1As shown, the microbial culture medium anaerobic control device 100 includes a gas storage tank 110, a deoxygenation device 120 and an anaerobic culture device 130. The gas storage tank 110, the deoxygenation device 120 and the anaerobic culture device 130 are connected in sequence. The gas storage tank 110 and the deoxygenation device 120 are both arranged at the front end of the anaerobic culture device 130, and the deoxygenation device 120 is arranged between the gas storage tank 110 and the anaerobic culture device 130.

[0047] The gas storage tank 110 is used to store gas. The gas stored in the gas storage tank 110 includes one or a combination of the following: nitrogen, hydrogen, and inert gas.

[0048] It should be noted that inert gases, also known as rare gases, are group 0 elements on the periodic table. At room temperature and pressure, inert gases are colorless and odorless monatomic gases that are difficult to react chemically. Inert gases include helium (He), neon (Ne), and argon (Ar).

[0049] When the type of gas is one, the gas is any one of nitrogen, hydrogen and an inert gas; when the type of gas is multiple, the gas is any combination of nitrogen, hydrogen and an inert gas.

[0050] The deoxidation device 120 is used to place a deoxidizer 124. The deoxidizer 124 is also called a deoxidizer. The deoxidizer 124 includes one or a combination of the following: sodium hydride and sodium thiocyanate.

[0051] When the deoxidizer 124 is of one kind, the deoxidizer 124 is any one of sodium hydride and sodium thiocyanate; when the deoxidizer 124 is of two kinds, the deoxidizer 124 includes sodium hydride and sodium thiocyanate.

[0052] like Figure 1 As shown, the anaerobic culture device 130 includes an anaerobic culture bottle 131 , a bottle cap 132 and a gas washing pipe assembly 170 .

[0053] The anaerobic culture bottle 131 can be in any shape according to actual needs. The anaerobic culture bottle 131 is made of transparent glass, which is convenient for experimenters to observe the bubbles inside.

[0054] The anaerobic culture bottle 131 has a culture chamber 1311 and a first opening 1312 , and the first opening 1312 is connected to the culture chamber 1311 . An experimenter can add culture medium into the culture chamber 1311 of the anaerobic culture bottle 131 through the first opening 1312 .

[0055] The bottle cap 132 is detachably connected to the anaerobic culture bottle 131. Optionally, the bottle cap 132 is detachably connected to the anaerobic culture bottle 131 by threaded connection. This method makes it convenient for experimenters to disassemble and assemble the bottle cap 132.

[0056] When the bottle cap 132 is connected to the anaerobic culture bottle 131, the bottle cap 132 is used to block the first opening 1312, and the bottle cap 132 is sealed and connected to the anaerobic culture bottle 131. By ensuring the sealing of the bottle cap 132 to the first opening 1312, it is effectively prevented that the outside air enters the anaerobic culture bottle 131 and causes contamination.

[0057] like Figure 1 , Figure 2 and Figure 3 As shown, the air wash pipe assembly 170 includes an air inlet pipe 133 , a porous structure 134 , an air wash pipe 135 and an air outlet pipe 136 .

[0058] The air inlet pipe 133 has a first end 1331 and a second end 1332. The first end 1331 of the air inlet pipe 133 is used to connect to the deoxygenation device 120 through the air inlet hose 140. At least a portion of the air inlet pipe 133 is passed through the bottle cap 132, and the second end 1332 can extend into the culture chamber 1311. In other words, the first end 1331 of the air inlet pipe 133 is connected to the deoxygenation device 120, and the second end 1332 of the air inlet pipe 133 passes through the bottle cap 132 and the first opening 1312, and extends into the culture chamber 1311.

[0059] The porous structure 134 is connected to the second end 1332 and is disposed in the culture chamber 1311. It should be noted that the porous structure 134 is sealedly connected to the second end 1332 and is relatively fixed to the air inlet pipe 133. Optionally, the porous structure 134 is a "cylindrical sand core microporous structure".

[0060] Optionally, the porous structure 134 is cylindrical as a whole and is stably arranged on the bottom wall of the culture chamber 1311. The porous structure 134 has a plurality of uniform air holes 1342 with small pore sizes and uniform distribution. The size of the uniform air holes 1342 determines the speed at which the gas passes and the size of the bubbles, thereby affecting the contact efficiency between the gas and the culture medium. By limiting the pore size of the uniform air holes 1342, smaller bubbles can be produced, increasing the contact area between the gas and the culture medium, which is conducive to the rapid diffusion of oxygen from the culture medium into the bubbles and being taken out of the culture system; at the same time, the uniformly distributed uniform air holes 1342 can ensure the uniform dispersion of the gas in the culture medium, avoiding the local uneven gas flow that affects the deoxygenation effect or the stability of the microbial growth environment.

[0061] In addition, the porous structure 134 is made of sand core material. The sand core material has good chemical stability, will not chemically react with the components in the culture medium, and will not have adverse effects on the growth of microorganisms, thereby ensuring safety and reliability during the microorganism cultivation process.

[0062] It should be noted that, when the porous structure 134 is a cylinder, the radial dimension of the porous structure 134 is larger than the radial dimension of the air inlet pipe 133. In this design, the porous structure 134 and the culture medium can have a larger contact area, and the gas discharged from the uniform gas hole 1342 can form a more uniform bubble flow in the culture medium, which is conducive to full contact between the gas and the culture medium.

[0063] One end of the air washing pipe 135 is provided with a blocking structure 1351. The air washing pipe 135 is sleeved on the air inlet pipe 133. At least a part of the air washing pipe 135 is passed through the bottle cap 132, and the other end of the air washing pipe 135 can extend into the culture chamber 1311.

[0064] Specifically, the washing pipe 135 has a third end and a fourth end that are arranged opposite to each other, wherein the third end of the washing pipe 135 is designed to be closed (with a blocking structure 1351); and the fourth end of the washing pipe 135 is provided with a second opening. The radial dimension of the washing pipe 135 is greater than the radial dimension of the air inlet pipe 133, and the washing pipe 135 is sleeved on the air inlet pipe 133. The fourth end of the washing pipe 135 can pass through the bottle cap 132 and the first opening 1312, and extend into the culture chamber 1311.

[0065] It should be noted that after the culture medium is added into the culture chamber 1311 of the anaerobic culture bottle 131 through the first opening 1312 , the liquid level of the culture medium exceeds the porous structure 134 and the second end 1332 of the air inlet pipe 133 , but does not exceed the second opening of the air wash pipe 135 .

[0066] An air outlet cavity 1352 is formed between the inner tube wall of the air washing pipe 135 and the outer tube wall of the air inlet pipe 133. An air outlet 1353 is formed on the side wall of the air washing pipe 135, and the air outlet 1353 is communicated with the air outlet cavity 1352.

[0067] It should be noted that the air wash pipe 135 is sealed and connected to the air inlet pipe 133 to prevent gas leakage or external air from entering and causing disturbance.

[0068] One end of the air outlet pipe 136 is connected to the air outlet 1353. Optionally, the air outlet pipe 136 is sealedly connected to the air outlet 1353 to prevent gas leakage or external air from entering and causing disturbance.

[0069] In a specific embodiment, the air wash pipe 135 and the air outlet pipe 136 are an integrated structure, which has good mechanical properties, higher connection strength and sealing performance than the post-assembly method, and is conducive to reducing the number of parts and components, thereby improving assembly efficiency.

[0070] In a specific embodiment, the air wash pipe 135 and the air outlet pipe 136 are an integrated structure, which has good mechanical properties, higher connection strength and sealing performance than the post-assembly method, and is conducive to reducing the number of parts and components, thereby improving assembly efficiency.

[0071] The gas in the gas storage tank 110 passes through the deoxygenation device 120 and the anaerobic culture device 130. The specific flow path of the gas in the anaerobic culture device 130 is: the first end 1331 of the air inlet pipe 133, the second end 1332 of the air inlet pipe 133, the porous structure 134, the culture chamber 1311, the second opening, the air outlet chamber 1352, the air outlet 1353 and the air outlet pipe 136.

[0072] Optionally, the other end of the gas outlet pipe 136 (the end away from the gas outlet 1353) is used to connect a gas collection device. By setting up a gas collection device, the gas can be collected to avoid being directly discharged into the atmosphere. In addition, the collected gas can be recycled, which is conducive to reducing the experimental cost.

[0073] In a specific embodiment, the other end of the gas outlet pipe 136 is used to connect to a gas outlet hose, and is connected to a gas collection device through the gas outlet hose. The gas collection device is a balloon, which has low cost and is convenient for recycling.

[0074] The first end 1331 of the air inlet pipe 133 is connected to the deoxygenation device 120 through the air inlet hose 140. The air inlet hose 140 includes a first pipe section 141 and a second pipe section 142. One end of the first pipe section 141 is connected to the deoxygenation device 120, and one end of the second pipe section 142 is connected to the first end 1331. The first pipe section 141 and the second pipe section 142 are detachably connected, which is convenient for staff to disassemble and assemble. When the first pipe section 141 and the second pipe section 142 are in a connected state, the deoxygenation device 120 and the anaerobic culture device 130 are in a connected state; when the first pipe section 141 and the second pipe section 142 are in a disconnected state, the deoxygenation device 120 and the anaerobic culture device 130 are in a disconnected state (not connected).

[0075] like Figure 1 and Figure 5 As shown, the microbial culture medium anaerobic control device 100 further includes a connecting valve 150 , a first control valve 161 , a second control valve 162 and a third control valve 163 .

[0076] The connecting valve 150 is used to connect the first pipe section 141 and the second pipe section 142. The first pipe section 141 and the second pipe section 142 are detachably connected via the connecting valve 150.

[0077] It should be noted that when the first pipe section 141 and the connecting valve 150 are in a connected state, the first pipe section 141 and the connecting valve 150 are sealed connected; when the second pipe section 142 and the connecting valve 150 are in a connected state, the second pipe section 142 and the connecting valve 150 are sealed connected.

[0078] In a specific embodiment, Figure 5 As shown, one end of the connecting valve 150 is a connecting sub-head 151, and the other end of the connecting valve 150 is a connecting female head 152. At least a portion of the connecting sub-head 151 is disposed in the first pipe section 141, and the connecting sub-head 151 and the first pipe section 141 are sealed and connected; at least a portion of the second pipe section 142 is disposed in the connecting female head 152, and the connecting female head 152 and the second pipe section 142 are sealed and connected.

[0079] The first control valve 161 is provided at the first pipe section 141, and the first control valve 161 is used to control the on-off of the first pipe section 141. The second control valve 162 is provided at the second pipe section 142, and the second control valve 162 is used to control the on-off of the second pipe section 142. The third control valve 163 is provided at the air outlet pipe 136, and the third control valve 163 is used to control the on-off of the air outlet pipe 136.

[0080] Optionally, the third control valve 163 is disposed on the air outlet hose, and the third control valve 163 controls the on-off of the air outlet hose and thus controls the on-off of the air outlet pipe 136 .

[0081] It should be noted that "on and off" means connected and disconnected. "Controlled on and off" means switching between the two states of connected and disconnected.

[0082] The steps of using the microbial culture medium anaerobic control device 100 are as follows: The first step is to close each control valve (close the first control valve 161, the second control valve 162 and the third control valve 163), dissolve the culture medium and pour it into the anaerobic culture bottle 131, fix the gas washing pipe 135, place the cylindrical sand core micropore (porous structure 134) at the bottom of the anaerobic culture bottle 131, and tighten the bottle cap 132 to make the anaerobic culture bottle 131 in a closed state.

[0083] The second step is to slowly release the gas (such as nitrogen, etc.) in the gas storage tank 110 and open the control valve (third control valve 163) on the gas outlet hose to ensure the effective release of gas pressure during the culture process and prevent the anaerobic culture bottle 131 from rupturing due to excessive pressure.

[0084] The third step is to observe the exhaust state of the porous structure 134 to ensure that the gas can fully contact the culture medium and be discharged through the exhaust port (i.e., the outlet 1353).

[0085] In the fourth step, after the culture medium is fully washed with gas (such as nitrogen, etc.) to ensure that the oxygen (O2) in the anaerobic culture bottle 131 is effectively discharged, the control valve (third control valve 163) on the gas outlet hose is closed to stop releasing gas. After the culture medium tends to be stable, the valves (first control valve 161 and second control valve 162) at the connection between the anaerobic culture bottle 131 and the deoxygenation device 120 are closed, and the connecting valve 150 is disconnected to isolate air from entering the anaerobic culture bottle 131, thereby ensuring the safe and stable operation of the anaerobic culture device 130.

[0086] Step 5: Transfer the anaerobic culture bottle 131 into a sterile glove box for the next inoculation operation to ensure that the entire culture process is strictly sealed.

[0087] It should be noted that during the culture process, the deoxidizer 124 is observed to change from light to dark in color until it turns completely black, and then the use is stopped and replaced.

[0088] The present invention aims to provide an anaerobic control device 100 for a microbial culture medium. Firstly, a deoxidizer 120 and an anaerobic culture device 130 are separately arranged, so that the oxygen in the culture system can be consumed by a deoxidizer 124, and the residual deoxidizer 124 can be prevented from chemically reacting with the culture medium or anaerobic microorganisms, which is beneficial to ensuring the growth rate of anaerobic microorganisms and the stability of the experiment. Secondly, a gas storage tank 110 and a deoxidizer 120 are arranged at the same time, and the deoxidizer 120 is arranged between the gas storage tank 110 and the anaerobic culture device 130. Before the gas (nitrogen, hydrogen or inert gas) enters the anaerobic culture bottle 131 of the anaerobic culture device 130, the deoxidizer 124 can remove the residual oxygen in the gas, which is beneficial to ensuring that nitrogen, hydrogen or inert gas replaces oxygen in the culture system to create anaerobic conditions. Thirdly, a gas washing pipe 135 is sleeved on the air inlet pipe 133. This design method is beneficial to optimizing the spatial layout and making the overall structure more compact.

[0089] It should be emphasized that the air wash pipe assembly 170 is composed of an air inlet pipe 133, a porous structure 134, an air wash pipe 135 and an air outlet pipe 136. By optimizing the spatial layout of the air wash pipe assembly 170, the overall structure can be made more compact.

[0090] For the cultivation of strictly anaerobic microorganisms, it is necessary to eliminate oxygen in the culture system. Even the presence of trace amounts of oxygen can cause irreversible inactivation or even death of strictly anaerobic microorganisms. Therefore, oxygen control technology is critical for the cultivation of strictly anaerobic microorganisms.

[0091] It should be noted that strict anaerobic microorganisms refer to a type of microorganisms that can only grow and reproduce under anaerobic conditions and are extremely sensitive to oxygen. Strict anaerobic microorganisms lack the enzyme systems necessary for survival in an aerobic environment (such as superoxide dismutase, catalase, etc.), and cannot effectively remove harmful reactive oxygen free radicals (such as superoxide anions, hydrogen peroxide, etc.) produced by oxygen in the cell. These reactive oxygen species will cause oxidative damage to the cell's biological macromolecules (such as proteins, nucleic acids, lipids, etc.), thereby destroying the cell's structural and functional integrity, and ultimately leading to its irreversible inactivation or even death.

[0092] Oxygen control technologies mainly include high-temperature deoxygenation technology, chemical deoxygenation technology, inert gas replacement technology and vacuum extraction technology.

[0093] Regarding high temperature deoxygenation technology: In conventional operation, the culture medium is placed in an anaerobic container (i.e., anaerobic culture bottle 131), the water in the culture medium is evaporated by heating to drive out dissolved oxygen, and the bottle is quickly sealed with a bottle cap 132 to block the entry of oxygen. It should be noted that the culture medium refers to a nutrient matrix prepared by combining different nutrients.

[0094] However, during the high-temperature deoxygenation process, the sealing of the bottle cap 132 will be affected to a certain extent and loosen, causing air to enter the culture container (i.e., the anaerobic culture bottle 131), thereby bringing about a risk of contamination; at the same time, the high temperature and high pressure conditions pose a safety hazard, and the anaerobic culture bottle 131 is prone to bursting, which is not conducive to the smooth progress of the culture process; and for some microorganisms that are highly sensitive to temperature, it even affects their growth and metabolic processes.

[0095] In the technical solution defined in the present invention, after the culture medium is loaded into the anaerobic culture bottle 131, it is not necessary to heat the culture medium, and the gas in the gas storage tank 110 enters the deoxygenation device 120 and the anaerobic culture bottle 131 in turn to create anaerobic conditions. In this way, firstly, it will not affect the sealing of the bottle cap 132 to the first opening 1312, and effectively prevent the outside air from entering the anaerobic culture bottle 131 to cause pollution; secondly, since it is not necessary to heat the culture medium, the temperature and pressure in the anaerobic culture bottle 131 are stable, which is conducive to ensuring the smooth progress of the culture process; thirdly, the temperature in the anaerobic culture bottle 131 is stable, so some microorganisms with strong temperature sensitivity can be cultured without affecting their growth and metabolic processes.

[0096] Regarding chemical deoxygenation technology: oxygen in the culture system is consumed by adding a deoxygenator (i.e., deoxidizer 124) to the anaerobic culture bottle 131. Although this technology can effectively deoxygenate, the deoxygenation rate is uncontrollable to a certain extent, and the residual deoxygenator will chemically react with the culture medium or microorganisms, thereby inhibiting the growth of microorganisms and affecting the stability of the experiment.

[0097] In the technical solution defined in the present invention, the deoxygenation device 120 and the anaerobic culture device 130 are separately arranged, which can not only consume the oxygen in the culture system through the deoxidizer 124, but also avoid the residual deoxidizer 124 from chemically reacting with the culture medium or anaerobic microorganisms, which is beneficial to ensure the growth rate of anaerobic microorganisms and the stability of the experiment.

[0098] It should be noted that the deoxidizer 124 includes but is not limited to sodium hydride or sodium thiocyanate.

[0099] About the inert gas replacement technology: By introducing inert gas into the anaerobic culture bottle 131, the oxygen in the culture system is replaced to create anaerobic conditions. This technology has high requirements for gas flow and ratio. Imprecise control will cause uneven gas flow in the culture system and affect the normal growth of microorganisms. At the same time, there is a small amount of O2 (oxygen) in general inert gas, which reduces the purity of the inert gas and affects the deoxygenation efficiency.

[0100] In the technical solution defined in the present invention, a gas storage tank 110 and a deoxygenation device 120 are provided at the same time, and the deoxygenation device 120 is provided between the gas storage tank 110 and the anaerobic culture device 130. Before the gas (nitrogen, hydrogen or inert gas) enters the anaerobic culture bottle 131 of the anaerobic culture device 130, the deoxidizer 124 can remove the residual oxygen in the gas, which is conducive to ensuring that nitrogen, hydrogen or inert gas replaces oxygen in the culture system to create anaerobic conditions. In addition, by accurately controlling the flow rate and ratio of the gas, the gas flow in the culture system is ensured to be uniform, effectively avoiding affecting the normal growth of microorganisms.

[0101] Regarding vacuum pumping technology: Although this technology can effectively eliminate oxygen, it has high requirements on equipment and needs to be equipped with vacuum pumps and gas control facilities, which increases the cost of experiments and equipment maintenance. At the same time, the pumping steps are numerous and complicated, making it difficult to maintain stable gas removal.

[0102] In the technical solution defined in the present invention, vacuum extraction technology is not used. Instead, the gas in the gas storage tank 110 enters the deoxygenation device 120 and the anaerobic culture bottle 131 in sequence. Therefore, there is no need to equip a vacuum pump and related facilities, which is conducive to controlling experimental costs, reducing experimental steps, and reducing experimental difficulty.

[0103] It should be emphasized that in the technical solution of the present invention, the gas storage tank 110 and the deoxygenation device 120 are both arranged at the front end of the anaerobic culture device 130, and the deoxygenation device 120 is arranged between the gas storage tank 110 and the anaerobic culture device 130. In this way, the oxygen in the gas (nitrogen, hydrogen or inert gas) can be effectively removed, and the residual oxygen in the culture system can be replaced. In addition, the deoxygenation device 120 and the anaerobic culture device 130 are arranged separately, that is, the deoxidizer 124 is arranged separately from the culture medium to avoid direct contact, thereby ensuring the normal growth and metabolism of anaerobic microorganisms and the stability of the experiment.

[0104] In some embodiments, optionally, Figure 2 and Figure 3 As shown, the porous structure 134 includes a first shell 1341. A gas-uniform cavity 1343 is provided in the first shell 1341. The first shell 1341 is connected to the second end 1332, and the air inlet pipe 133 is connected to the gas-uniform cavity 1343. A plurality of gas-uniform holes 1342 are provided on the first shell 1341, and the gas-uniform holes 1342 are connected to the gas-uniform cavity 1343.

[0105] It should be noted that the porous structure 134 is sealed and connected to the second end 1332 of the air inlet pipe 133 to ensure that the gas flow path is: the second end 1332 of the air inlet pipe 133, the gas uniforming cavity 1343, the gas uniforming holes 1342 and the culture cavity 1311.

[0106] The porous structure 134 is cylindrical as a whole and is stably arranged on the bottom wall of the culture chamber 1311. The porous structure 134 has a plurality of uniform air holes 1342 with small pore sizes and uniform distribution. The size of the uniform air holes 1342 determines the speed at which the gas passes and the size of the bubbles, thereby affecting the contact efficiency between the gas and the culture medium. By limiting the pore size of the uniform air holes 1342, smaller bubbles can be produced, increasing the contact area between the gas and the culture medium, which is conducive to the rapid diffusion of oxygen from the culture medium into the bubbles and being taken out of the culture system; at the same time, the uniformly distributed uniform air holes 1342 can ensure the uniform dispersion of the gas in the culture medium, avoiding the local uneven gas flow that affects the deoxygenation effect or the stability of the microbial growth environment.

[0107] In addition, the porous structure 134 is made of sand core material. The sand core material has good chemical stability, will not chemically react with the components in the culture medium, and will not have adverse effects on the growth of microorganisms, thereby ensuring safety and reliability during the microorganism cultivation process.

[0108] It should be noted that, when the porous structure 134 is a cylinder, the radial dimension of the porous structure 134 is larger than the radial dimension of the air inlet pipe 133. In this design, the porous structure 134 and the culture medium can have a larger contact area, and the gas discharged from the uniform gas hole 1342 can form a more uniform bubble flow in the culture medium, which is conducive to full contact between the gas and the culture medium.

[0109] In some embodiments, optionally, at least a portion of the air inlet pipe 133 passes through the first shell 1341 , and the second end 1332 is disposed in the air uniforming cavity 1343 , and the air inlet pipe 133 is sealedly connected to the first shell 1341 .

[0110] In other words, the second end 1332 of the air inlet pipe 133 can pass through the shell wall of the first shell 1341 and extend into the culture chamber 1311 so that the lumen of the air inlet pipe 133 is in communication with the gas homogenizing chamber 1343 .

[0111] By setting the air inlet pipe 133 and the first shell 1341 as a sealed connection, it is ensured that the gas passes through the second end 1332 of the air inlet pipe 133, the gas uniformity cavity 1343, the gas uniformity hole 1342 and the culture cavity 1311 in sequence, which is conducive to sufficient contact between the gas and the culture medium.

[0112] In some embodiments, optionally, Figure 2 As shown, the bottle cap 132 includes a first portion 1321 and a second portion 1322. The second portion 1322 is an annular structure, and the second portion 1322 is wound around the first portion 1321.

[0113] Optionally, the first part 1321 and the second part 1322 are an integrated structure, that is, the bottle cap 132 is an integral structure, which has good mechanical properties, higher connection strength and sealing performance than the post-assembly method, and is conducive to reducing the number of parts and improving assembly efficiency.

[0114] The second portion 1322 and the first portion 1321 define a mounting groove 1323. At least a portion of the anaerobic culture bottle 131 is disposed in the mounting groove 1323 to achieve a detachable connection between the bottle cap 132 and the anaerobic culture bottle 131. When the bottle cap 132 and the anaerobic culture bottle 131 are in a connected state, the first portion 1321 can abut against the first opening 1312 of the anaerobic culture bottle 131 to block the first opening 1312.

[0115] When the bottle cap 132 is connected to the anaerobic culture bottle 131, the bottle cap 132 is sealed to the anaerobic culture bottle 131. By ensuring the sealing of the bottle cap 132 to the first opening 1312, it is effectively prevented that the outside air enters the anaerobic culture bottle 131 and causes contamination.

[0116] like Figure 2As shown, the first part 1321 is provided with a notch 1324, and one end of the gas washing pipe 135 away from the blocking structure 1351 can extend into the culture chamber 1311 through the notch 1324. The notch 1324 is used for the gas washing pipe 135 to pass through, and the gas washing pipe 135 is sealed and connected to the inner wall of the notch 1324.

[0117] In a specific embodiment, the groove wall of the installation groove 1323 is provided with an internal thread, and the first opening 1312 of the anaerobic culture bottle 131 is provided with an external thread. The bottle cap 132 and the anaerobic culture bottle 131 are threadedly connected by the internal thread and the external thread.

[0118] In some embodiments, optionally, Figure 2 As shown, the anaerobic culture device 130 further includes a sealing pressure ring 137 . The sealing pressure ring 137 is disposed in the mounting groove 1323 , and the sealing pressure ring 137 is disposed between the first opening 1312 and the first portion 1321 .

[0119] By providing the sealing pressure ring 137 , when the bottle cap 132 and the anaerobic culture bottle 131 are in a connected state, the sealing performance of the bottle cap 132 with respect to the first opening 1312 can be further improved, thereby effectively preventing external air from entering the anaerobic culture bottle 131 and causing contamination.

[0120] In some embodiments, optionally, Figure 4 As shown, the deoxidation device 120 includes a deoxidation tube 121, a first end cap 122 and a second end cap 123. The deoxidation tube 121 is used to place a deoxidizer 124. The first end cap 122 is provided at one end of the deoxidation tube 121, and the first end cap 122 is used to connect one end of the first pipe section 141; the second end cap 123 is provided at the other end of the deoxidation tube 121, and the second end cap 123 is used to connect the gas storage tank 110.

[0121] By setting up the deoxygenation device 120 separately (the deoxygenation device 120 and the anaerobic culture device 130 are set separately), on the one hand, it is possible to avoid chemical reactions between the residual deoxidizer 124 and the culture medium or anaerobic microorganisms, which is beneficial to ensure the growth rate of anaerobic microorganisms and the stability of the experiment; on the other hand, it is convenient for the experimenter to replace the deoxidizer 124 in time.

[0122] Stop using the deoxidizer 124 and replace it after observing the color of the deoxidizer 124 change from light to dark until it turns completely black.

[0123] In some embodiments, the first end cap 122 is optionally detachably connected to the deoxidation tube 121. This design makes it convenient for the staff to disassemble and assemble the first end cap 122 to open or close the deoxidation tube 121.

[0124] When the first end cap 122 and the deoxidation tube 121 are in a connected state, the first end cap 122 and the deoxidation tube 121 are sealed and connected, and at this time, the first tube section 141 is in communication with the cavity of the deoxidation tube 121 .

[0125] When the first end cap 122 and the deoxidation tube 121 are in a separated state, the staff can replace the deoxidizer 124 in time to ensure that oxygen in the gas is removed.

[0126] In some embodiments, the second end cap 123 is optionally detachably connected to the deoxidation tube 121. This design makes it convenient for the staff to disassemble and assemble the second end cap 123 to open or close the deoxidation tube 121.

[0127] When the second end cap 123 and the deoxidation tube 121 are in a connected state, the second end cap 123 and the deoxidation tube 121 are sealed and connected, and the gas in the gas storage tank 110 can enter the cavity of the deoxidation tube 121 .

[0128] When the second end cap 123 and the deoxidation tube 121 are in a separated state, the staff can replace the deoxidizer 124 in time to ensure that oxygen in the gas is removed.

[0129] In some embodiments, optionally, the microbial culture medium anaerobic control device 100 includes a gas storage tank 110, a deoxygenation device 120, and an anaerobic culture device 130 that are sequentially connected.

[0130] Taking nitrogen as an example, the deoxygenator 120 is disposed at the front end of the anaerobic culture device 130 to remove the oxygen content in the nitrogen and replace the residual oxygen in the culture system. The deoxygenator 124 and the culture medium are placed separately to avoid direct contact. In addition, the deoxygenator 120 uses a detachable sealing cover (the first end cap 122 or the second end cap 123), which is convenient for timely replacement of the deoxygenator 124, and is conducive to improving the ease of operation.

[0131] The wash pipe 135 is sleeved on the air inlet pipe 133, and the second end 1332 of the air inlet pipe 133 is provided with a cylindrical sand core micropore (porous structure 134). The cylindrical sand core micropore is placed at the bottom of the anaerobic culture bottle 131 and is configured to exhaust from bottom to top to increase the full contact between nitrogen and the culture medium and ensure that oxygen is quickly discharged. The top of the wash pipe 135 adopts an open exhaust port to ensure the balance of air pressure inside and outside the anaerobic culture bottle 131. The gas flow rate is measured according to the aeration amplitude of the micropore (uniform air hole 1342) to ensure the uniformity of gas in the anaerobic culture bottle 131.

[0132] The deoxygenation device 120 and the anaerobic culture device 130 are detachably connected via the air intake hose 140 and the connecting valve 150, ensuring that the anaerobic culture bottle 131 can move freely when the anaerobic environment is reached. At the same time, the multi-end control valve setting (specifically including the first control valve 161, the second control valve 162 and the third control valve 163) in the microbial culture medium anaerobic control device 100 can enable the anaerobic culture bottle 131 to maintain an anaerobic state for a long time.

[0133] The microbial culture medium anaerobic control device 100 provided by the present invention is intended to improve the deoxygenation efficiency and maintain the anaerobic state. By improving the airtightness, ease of operation and cost effectiveness of the device, it has the following technical advantages (taking the gas stored in the gas storage tank 110 as nitrogen as an example): (1) The deoxygenation device 120 is disposed at the front end of the anaerobic culture device 130 to remove the oxygen content in the nitrogen, thereby accelerating the gas replacement efficiency in the device and reducing the nitrogen consumption.

[0134] (2) The deoxidizer 124 is placed separately from the culture medium, which helps to reduce the risk of contamination of the culture medium by the chemical reaction of the deoxidizer 124.

[0135] (3) The deoxidation device 120 is independently arranged, which is convenient for regular replacement of the deoxidizer 124, thereby improving the utilization efficiency of the device and reducing maintenance risks.

[0136] (4) The second end 1332 of the air inlet pipe 133 is provided with a porous structure 134, which disperses the gas through a plurality of gas-uniform holes 1342, so that the nitrogen gas is fully in contact with the culture medium, thereby quickly removing oxygen.

[0137] (5) The top of the air washing pipe 135 is designed with an open exhaust port so that the anaerobic culture device 130 maintains a normal internal air pressure, which helps to reduce the risk of the anaerobic culture bottle 131 rupturing due to a large ventilation pressure.

[0138] (6) The deoxygenation device 120 and the anaerobic culture device 130 are designed to be detachable, which is conducive to the free movement of the anaerobic culture bottle 131 when the anaerobic state is reached, so as to carry out the next inoculation and culture operation.

[0139] (7) By providing the first control valve 161 , the second control valve 162 , and the third control valve 163 , it is possible to ensure that the anaerobic culture bottle 131 maintains an anaerobic state for a short period of time during the experiment.

[0140] (8) The overall design of the microbial culture medium anaerobic control device 100 is simple and easy to assemble and disassemble. It can be directly placed on a laboratory bench for use without the need for auxiliary equipment such as a clean bench, a vacuum pumping device, and an anaerobic glove box.

[0141] (9) The microbial culture medium anaerobic control device 100 has low energy consumption and does not require the intervention of additional auxiliary energy such as thermal energy and electrical energy.

[0142] (10) The microbial culture medium anaerobic control device 100 has high safety. The stability of the internal pressure is ensured by the multi-end control valve (specifically, the first control valve 161, the second control valve 162 and the third control valve 163) and the open design of the anaerobic culture bottle 131.

[0143] In some embodiments, optionally, the air intake pipe 133 is connected to a first hose (the air intake hose 140 ), and a first pipe section 141 of the air intake hose 140 is provided with a first control valve 161 ; and a second pipe section 142 of the air intake hose 140 is provided with a second control valve 162 .

[0144] One end of the air inlet hose 140 away from the anaerobic culture bottle 131 is sealed and connected to the deoxygenation device 120. The air outlet 1353 is connected to the air outlet hose. A third control valve 163 is provided on the air outlet hose.

[0145] In the embodiment of the present invention, the bottle cap 132 adopts an open design, that is, the bottle cap 132 is provided with a mounting groove 1323. The bottle cap 132 is detachably connected to the anaerobic culture bottle 131, and the bottle cap 132 is used to block the first opening 1312. A sealing pressure ring 137 is provided between the anaerobic culture bottle 131 and the bottle cap 132 to improve the sealing performance between the anaerobic culture bottle 131 and the bottle cap 132.

[0146] A through hole is provided on the sealing pressing ring 137. The outer profile of the gas washing pipe 135 passes through the through holes of the bottle cap 132 and the sealing pressing ring 137 and extends into the interior of the anaerobic culture bottle 131.

[0147] In the embodiment of the present invention, the sealing pressure ring 137 is annular and is disposed corresponding to the anaerobic culture bottle 131 and the bottle cap 132 .

[0148] In the embodiment of the present invention, the air inlet pipe 133 and the air outlet 1353 are both provided with threaded connectors at the ends away from the anaerobic culture bottle 131. The air inlet pipe 133 is sealedly connected to the air inlet hose 140 through the threaded connector; the air outlet 1353 is sealedly connected to the air outlet hose through the threaded connector.

[0149] In an embodiment of the present invention, the threaded connector includes a rubber plug and a connection thread cover. The connection thread cover is connected to the air inlet pipe 133.

[0150] It should be noted that the threaded connector and the connecting threaded cover are both made of PBT (Polybutyleneterephthalate).

[0151] In the embodiment of the present invention, the air inlet hose 140 , the air outlet hose and the sealing ring 137 are all made of silicone.

[0152] In the embodiment of the present invention, the deoxidizer 120 is arranged longitudinally, with the lower end being the air inlet end and the upper end being the air outlet end. The deoxidizer 124 is installed inside the deoxidizer 120, and can be replaced regularly by closing the sealing cover (the first end cap 122 or the second end cap 123).

[0153] In the embodiment of the present invention, a first control valve 161, a second control valve 162 and a connecting valve 150 are provided between the deoxygenation device 120 and the anaerobic culture device 130. By closing the control valves and disconnecting the female connector 152 and the female connector 151 of the connecting valve 150, the deoxygenation device 120 and the anaerobic culture device 130 can be separated.

[0154] In one embodiment according to the present invention, the control method is used for the microbial culture medium anaerobic control device 100 in any of the above embodiments.

[0155] like Figure 6 As shown, the steps of the control method include: S202, close the first control valve, the second control valve and the third control valve in the microbial culture medium anaerobic control device.

[0156] This step is the preparation work. Close all control valves (close the first control valve, the second control valve and the third control valve), dissolve the culture medium and pour it into the anaerobic culture bottle, fix the gas washing pipe, place the cylindrical sand core micropore (porous structure) at the bottom of the anaerobic culture bottle, and tighten the bottle cap to make the anaerobic culture bottle in a closed state.

[0157] S204, when it is determined that the culture medium is in the anaerobic culture bottle of the anaerobic culture device of the microbial culture medium anaerobic control device, open the first control valve and the second control valve to allow the gas in the gas storage tank of the microbial culture medium anaerobic control device to enter the deoxygenation device and the anaerobic culture bottle of the microbial culture medium anaerobic control device in turn.

[0158] The purpose of this step is to release the gas (such as nitrogen, etc.) in the gas storage tank so that the gas in the gas storage tank can replace the air in the anaerobic control device for the microbial culture medium.

[0159] S206, opening the third control valve after the first time period.

[0160] Slowly release the gas (such as nitrogen, etc.) in the gas storage tank and open the control valve (the third control valve) on the gas outlet hose to ensure the effective release of gas pressure during the culture process and prevent the anaerobic culture bottle from rupturing due to excessive pressure.

[0161] S208, closing the first control valve, the second control valve and the third control valve after a second time period; wherein the second time period is greater than the first time period.

[0162] After the culture medium is fully washed with gas (such as nitrogen, etc.) to ensure that the oxygen (O2) in the anaerobic culture bottle is effectively discharged, close the control valve (the third control valve) on the gas outlet hose to stop releasing gas. After the culture medium becomes stable, close the valves (the first control valve and the second control valve) connecting the anaerobic culture bottle and the deoxygenation device, and disconnect the connecting valve to isolate air from entering the anaerobic culture bottle to ensure the safe and stable operation of the anaerobic culture device.

[0163] Transfer the anaerobic culture bottle into a sterile glove box for the next inoculation operation to ensure that the entire culture process is strictly sealed.

[0164] In the technical solution defined in the present invention, the gas in the gas storage tank enters the deoxygenation device and the anaerobic culture device in turn. In this design, on the one hand, the deoxygenation device and the anaerobic culture device are set separately, which can not only consume the oxygen in the culture system through the deoxygenator, but also avoid the residual deoxygenator from chemically reacting with the culture medium or anaerobic microorganisms, which is beneficial to ensure the growth rate of anaerobic microorganisms and the stability of the experiment; on the other hand, the gas storage tank and the deoxygenation device are set at the same time, and the deoxygenation device is set between the gas storage tank and the anaerobic culture device. Before the gas (nitrogen, hydrogen or inert gas) enters the anaerobic culture bottle of the anaerobic culture device, the deoxygenation agent can remove the residual oxygen in the gas, which is beneficial to ensure that nitrogen, hydrogen or inert gas replaces the oxygen in the culture system to create anaerobic conditions.

[0165] It should be noted that, because the control method is used for the anaerobic control device for microbial culture medium in any of the above embodiments, the control method has the beneficial effects of any of the above embodiments and will not be described in detail here.

[0166] In one embodiment according to the present invention, Figure 7 As shown, the electronic device 300 includes a memory 310 and a processor 320. The memory 310 stores a program or instruction that can be run on the processor 320, and the processor 320 implements the steps of the control method in the above embodiment when executing the program or instruction. The electronic device 300 has the beneficial effects of the above embodiment, which will not be repeated here.

[0167] In one embodiment of the present invention, the readable storage medium stores a program or instruction, and when the program or instruction is executed by the processor, the steps of the control method in the above embodiment are implemented. The readable storage medium has the beneficial effects of the above embodiment, which will not be repeated here.

[0168] In the present invention, the terms "first", "second", and "third" are used for descriptive purposes only and should not be understood as indicating or implying relative importance; the term "plurality" refers to two or more, unless otherwise clearly defined. The terms "installed", "connected", "connected", "fixed", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; "connected" can be a direct connection or an indirect connection through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0169] In the description of the present invention, it is necessary to understand that the directions or positional relationships indicated by terms such as “upper”, “lower”, “left”, “right”, “front” and “back” are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or unit referred to must have a specific direction, be constructed and operated in a specific orientation, and therefore, cannot be understood as a limitation on the present invention.

[0170] In the description of this specification, the description of the terms "one embodiment", "some embodiments", "specific embodiments", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0171] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A microbial culture medium anaerobic control device, characterized in that: It comprises a connected gas storage tank (110), a deoxygenation device (120) and an anaerobic culture device (130), wherein the gas storage tank (110) is used to store gas, which is nitrogen, hydrogen or an inert gas, and the deoxygenation device (120) is used to place a deoxidizer (124); The anaerobic culture device (130) comprises: An anaerobic culture bottle (131) having a culture chamber (1311) and a first opening (1312), wherein the first opening (1312) is in communication with the culture chamber (1311); a bottle cap (132) detachably connected to the anaerobic culture bottle (131); when the bottle cap (132) and the anaerobic culture bottle (131) are in a connected state, the bottle cap (132) is used to block the first opening (1312); The air scrubber assembly (170) includes: an air inlet pipe (133), the air inlet pipe (133) having a first end (1331) and a second end (1332), the first end (1331) being used to connect to the deoxygenation device (120) via an air inlet hose (140), at least a portion of the air inlet pipe (133) being passed through the bottle cap (132), and the second end (1332) being able to extend into the culture chamber (1311); A porous structure (134) connected to the second end (1332), and the porous structure (134) is disposed in the culture chamber (1311); a gas washing pipe (135), wherein one end of the gas washing pipe (135) is provided with a blocking structure (1351), the gas washing pipe (135) is sleeved on the gas inlet pipe (133), at least a portion of the gas washing pipe (135) is passed through the bottle cap (132), and the other end of the gas washing pipe (135) can extend into the culture chamber (1311), an air outlet cavity (1352) is formed between the inner tube wall of the gas washing pipe (135) and the outer tube wall of the gas inlet pipe (133), and a gas outlet (1353) is formed on the side wall of the gas washing pipe (135), and the gas outlet (1353) is communicated with the gas outlet cavity (1352); An air outlet pipe (136), one end of the air outlet pipe (136) being connected to the air outlet (1353); The air intake hose (140) comprises a first pipe section (141) and a second pipe section (142); one end of the first pipe section (141) is connected to the deoxygenation device (120), and one end of the second pipe section (142) is connected to the first end (1331); The microbial culture medium anaerobic control device also includes: a connecting valve (150), the connecting valve (150) being used to connect the first pipe section (141) and the second pipe section (142); a first control valve (161), provided on the first pipe section (141), the first control valve (161) being used to control the on-off of the first pipe section (141); a second control valve (162), provided on the second pipe section (142), the second control valve (162) being used to control the on-off of the second pipe section (142); A third control valve (163) is provided on the air outlet pipe (136), and the third control valve (163) is used to control the on-off of the air outlet pipe (136).

2. The anaerobic control device for microbial culture medium according to claim 1, characterized in that: The porous structure (134) comprises: A first shell (1341), wherein an air-uniform cavity (1343) is provided in the first shell (1341), the first shell (1341) is connected to the second end (1332), and the air inlet pipe (133) is in communication with the air-uniform cavity (1343), and a plurality of air-uniform holes (1342) are provided on the first shell (1341), and the air-uniform holes (1342) are in communication with the air-uniform cavity (1343).

3. The anaerobic control device for microbial culture medium according to claim 2, characterized in that: At least a portion of the air intake pipe (133) is disposed in the first shell (1341), and the second end (1332) is disposed in the air homogenizing cavity (1343); the air intake pipe (133) is sealedly connected to the first shell (1341).

4. The anaerobic control device for microbial culture medium according to any one of claims 1 to 3, characterized in that: The bottle cap (132) comprises: Part I (1321); A second part (1322), the second part (1322) is an annular structure, and the second part (1322) is arranged around the first part (1321), and the second part (1322) and the first part (1321) surround a mounting groove (1323); At least a portion of the anaerobic culture bottle (131) is disposed in the mounting groove (1323); the first portion (1321) is used to block the first opening (1312); a notch (1324) is provided on the first portion (1321); and an end of the gas washing pipe (135) away from the blocking structure (1351) can extend into the culture chamber (1311) through the notch (1324).

5. The anaerobic control device for microbial culture medium according to claim 4, characterized in that: The anaerobic culture device (130) further includes: A sealing pressure ring (137) is disposed in the mounting groove (1323); the sealing pressure ring (137) is disposed between the first opening (1312) and the first portion (1321).

6. The microbial culture medium anaerobic control device according to any one of claims 1 to 3, characterized in that: The deoxidation device (120) comprises: a deoxidation tube (121), wherein the deoxidation agent (124) is placed in the deoxidation tube (121); A first end cap (122) is provided at one end of the deoxidation tube (121), the first end cap (122) being used to connect to one end of the first tube section (141); A second end cap (123) is provided at the other end of the deoxidation tube (121), and the second end cap (123) is used to connect to the gas storage tank (110).

7. The anaerobic control device for microbial culture medium according to claim 6, characterized in that: The first end cap (122) is detachably connected to the deoxidation tube (121); and / or The second end cap (123) is detachably connected to the deoxidation tube (121).

8. A control method, characterized in that: The anaerobic control device for a microbial culture medium according to any one of claims 1 to 7, wherein the control method comprises: Closing the first control valve, the second control valve and the third control valve in the microbial culture medium anaerobic control device; When it is determined that the culture medium is in the anaerobic culture bottle of the anaerobic culture device of the microbial culture medium anaerobic control device, the first control valve and the second control valve are opened to allow the gas in the gas storage tank of the microbial culture medium anaerobic control device to enter the deoxygenation device of the microbial culture medium anaerobic control device and the anaerobic culture bottle in sequence; opening the third control valve after a first period of time; The first control valve, the second control valve and the third control valve are closed after a second time period; wherein the second time period is greater than the first time period.

9. An electronic device, characterized in that: include: A memory (310) and a processor (320), wherein the memory (310) stores a program or instruction that can be run on the processor (320), and the processor (320) implements the steps of the control method according to claim 8 when executing the program or the instruction.

10. A readable storage medium, characterized in that: The readable storage medium stores a program or an instruction, and when the program or the instruction is executed by a processor, the steps of the control method according to claim 8 are implemented.

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