Low-temperature preservation and ultralow-temperature preservation method for sperms of siniperca chuatsi subjected to feed domestication

Abstract

The invention provides a feed domesticated siniperca chuatsi sperm low-temperature preservation and ultralow-temperature preservation method, which comprises the following steps: firstly, providing a siniperca chuatsi sperm low-temperature preservation diluent which comprises Hanks liquid, sodium chloride, soya bean lecithin, vitamin E, curcumin, melatonin and SOD (superoxide dismutase); and adding an anti-freezing protective agent into the low-temperature preservation diluent to obtain the ultralow-temperature preservation anti-freezing liquid for the siniperca chuatsi sperms. The siniperca chuatsi semen and the low-temperature preservation diluent are uniformly mixed, so that low-temperature preservation can be realized; the siniperca chuatsi semen is uniformly mixed with the cryopreservation anti-freezing liquid, so that cryopreservation can be realized. The siniperca chuatsi sperm low-temperature preservation diluent is preferably selected, low-temperature preservation of the siniperca chuatsi sperms can be achieved, ultralow-temperature preservation can be conducted after an anti-freezing protective agent is added, the persistence of the preservation time of the siniperca chuatsi sperms can be guaranteed, the protection effect on the sperms is good, and the activation rate and activity of the unfrozen sperms are high.