Composition, application and skin care product with free radical scavenging effect

Abstract

The present application provides a composition, application and skin care product with the effect of scavenging free radicals. The composition with the effect of scavenging free radicals includes lipopeptides, yeast extract and water; the mass proportion of lipopeptides in the composition is 0.001wt%-99wt%, and the mass proportion of yeast extract in the composition is 0.01wt%-30wt%. Lipopeptides have the effect of scavenging free radicals and can eliminate the active oxygen generated in the body from the root; yeast extract and lipopeptides work synergistically to increase the scavenging rate of lipopeptides on free radicals, promote the regeneration and repair of skin cells, enhance the barrier function of the skin, and improve the resistance of the skin; at the same time, yeast extract can also promote the metabolism of epidermal keratinocytes, making the skin delicate and elastic.

Description

Technical Field

[0001] The present application relates to the technical field of cosmetics, and in particular to a composition, application and skin care product having the effect of scavenging free radicals. Background Art

[0002] The antioxidant capacity of the skin refers to the ability of the skin itself and the antioxidant ingredients obtained through the use of skin care products to jointly resist oxidative stress caused by free radicals. Free radicals are one of the main causes of skin aging, pigmentation, inflammation and other problems.

[0003] For the skin, free radicals will promote the cross-linking reaction of collagen, reduce the production of elastin, accelerate the decomposition of elastic fibers, and directly destroy hyaluronic acid, thereby affecting the moisture retention, tension, elasticity and softness of the dermis, and then causing skin aging and wrinkle formation. In addition, free radicals will attack human cells, causing peroxidation of phospholipids and unsaturated fatty acids on the cell membrane, and further combining with proteins to form lipofuscin. These lipofuscins accumulate in cells, interfere with the normal metabolism of cells, promote cell aging, and result in a dark complexion.

[0004] However, the antioxidant products currently on the market can easily put the skin in a state of oxidative stress, causing skin problems such as skin inflammation, erythema, wrinkles, and acne. Summary of the invention

[0005] Based on this, the present application provides a composition, application and skin care product with the effect of scavenging free radicals, so as to reduce the occurrence of skin oxidative stress while scavenging free radicals.

[0006] One or more embodiments of the present application provide a composition having the effect of scavenging free radicals, comprising lipopeptide, yeast extract and water; the mass proportion of the lipopeptide in the composition is 0.001wt%-99wt%, and the mass proportion of the yeast extract in the composition is 0.01wt%-30wt%.

[0007] In some embodiments, the mass proportion of the lipopeptide in the composition is 10wt%-50wt%; and / or

[0008] The yeast extract accounts for 5wt%-20wt% of the composition.

[0009] In some embodiments, the lipopeptide comprises one or more of surfactin, iturin, fenmucin, lichenin, babylonin, pamiramycin, sporomycin, mycosubtilin, phosphatidylinositol, fusaricide, kurstak, bacillus, polymyxin, octapeptidin and polypeptide bacteriocin.

[0010] In some embodiments, the composition further comprises a first humectant;

[0011] Optionally, the mass proportion of the first moisturizing agent in the composition is 0.001wt%-50wt%, and optionally 0.5wt%-10wt%;

[0012] Optionally, the first moisturizing agent includes one or more of trehalose, hydroxyethyl urea, mannan, sodium hyaluronate, bletilla striata polysaccharide, aloe polysaccharide, dendrobium officinale polysaccharide, glycerol polyoxyethylene ether, glycerol polyether-26 and polyoxyethylene-polyoxypropylene copolymer.

[0013] In some embodiments, the composition further comprises a first emollient;

[0014] Optionally, the mass proportion of the first emollient in the composition is 0.001wt%-50wt%, optionally 1wt%-10wt%;

[0015] Optionally, the first emollient includes one or more of olive oil, shea butter, behenyl myristate, caprylate, oleyl oleate, hexyl laurate, isopropyl palmitate, coconut oil, sunflower oil, corn oil, rapeseed oil, mineral esters, mineral oil, beeswax, lanolin, liquid paraffin, squalene, PEG-6 caprylic / capric triglyceride and dioctylcyclohexane.

[0016] In some embodiments, the composition further comprises a first pH adjuster;

[0017] Optionally, the mass proportion of the first pH adjuster in the composition is 0.001 wt%-20 wt%, and optionally 0.01 wt%-5 wt%;

[0018] Optionally, the first pH adjuster includes one or more of citric acid, malic acid, lactic acid, sodium hydroxide, potassium hydroxide, serine, glutamic acid, disodium hydrogen phosphate and arginine.

[0019] In some embodiments, the composition further comprises a polyol;

[0020] Optionally, the mass proportion of the polyol in the composition is 0.001wt%-30wt%;

[0021] Optionally, the polyol includes one or more of butylene glycol, propylene glycol, sorbitol, glycerol, pentylene glycol, polypropylene glycol and glycerol.

[0022] In some embodiments, the composition further comprises a first emulsifier;

[0023] Optionally, the mass proportion of the first emulsifier in the composition is 0.001wt%-50wt%, optionally 1wt%-10wt%;

[0024] Optionally, the first emulsifier includes one or more of sodium lauryl sulfate, potassium lauryl sulfate, octadecyltrimethylammonium bromide, PEG-100 stearate, steareth-2, steareth-21, glyceryl stearate, steareth-2, polyoxyethylene monostearate and lecithin.

[0025] In some embodiments, the composition further comprises a first preservative;

[0026] Optionally, the mass proportion of the first preservative in the composition is 0.001wt%-10wt%, optionally 0.01wt%-1wt%;

[0027] Optionally, the first preservative includes one or more of phenoxyethanol, p-hydroxyacetophenone, imidazolidinyl urea, diazolidinyl urea, sodium hydroxymethylglycinate, methylparaben, ethylparaben, propylparaben, potassium sorbate, methylisothiazolinone, DMDM ​​hydantoin, sodium benzoate and benzyl alcohol.

[0028] In some embodiments, the solvent includes deionized water.

[0029] The second aspect of the present application provides a use of the composition with free radical scavenging effect of the first aspect of the present application in the preparation of a skin care product with antioxidant effect.

[0030] The third aspect of the present application provides a skin care product, comprising the composition with free radical scavenging effect of the first aspect of the present application.

[0031] In some embodiments, the composition accounts for 0.1wt%-10wt% by weight of the skin care product, and can be optionally 0.5wt%-5wt%.

[0032] In some embodiments, based on 100 parts by mass of the skin care product, the skin care product comprises:

[0033] 0.1 to 10 parts of the composition, 10 to 20 parts of a second moisturizer, 8 to 15 parts of a second emollient, 0.5 to 3 parts of a second emulsifier, 0.02 to 0.06 parts of a second pH adjuster, 0.3 to 1 part of a second preservative, 0.1 to 0.3 parts of a thickener, 0.01 to 0.05 parts of a chelating agent, 0.05 to 0.2 parts of a fragrance, and 50 to 80 parts of deionized water.

[0034] In some embodiments, the skin care product has at least one of the following features (1)-(7):

[0035] (1) the second humectant comprises one or more of glycerin and butylene glycol; and / or

[0036] (2) the second emollient comprises one or more of isononyl isononanoate, dicaprylyl carbonate and polydimethylsiloxane;

[0037] (3) the second emulsifier includes one or more of arachidyl docosyl alcohol and arachidyl glucoside;

[0038] (4) the second pH adjuster includes sodium hydroxide;

[0039] (5) the second preservative comprises one or more of p-hydroxyacetophenone and caprylhydroxamic acid;

[0040] (6) The thickener includes acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer;

[0041] (7) The chelating agent includes disodium EDTA;

[0042] (8) The pH value of the skin care product is 6.8-7.4.

[0043] In some embodiments, the skin care product includes one or more of lotion, emulsion, cream, gel, essence, mask, gel and spray.

[0044] The above-mentioned composition with the effect of scavenging free radicals includes lipopeptides and yeast extracts. Lipopeptides have the effect of scavenging free radicals and can eliminate the active oxygen generated in the body from the root. Yeast extracts and lipopeptides work synergistically to improve the scavenging rate of lipopeptides on free radicals, promote the regeneration and repair of skin cells, enhance the barrier function of the skin, and improve the resistance of the skin. At the same time, yeast extracts can also promote the metabolism of epidermal keratinocytes, making the skin delicate and elastic. Therefore, the composition with the effect of scavenging free radicals can effectively reduce the occurrence of skin stress and improve the skin condition while effectively scavenging free radicals. DETAILED DESCRIPTION

[0045] For ease of understanding of the application, the application will be described more fully below with reference to the embodiments. The following provides preferred embodiments of the application. However, the application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the application more thorough and comprehensive.

[0046] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0047] In the present application, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0048] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0049] Only some numerical ranges are specifically disclosed herein. However, any lower limit can be combined with any upper limit to form an unspecified range; and any lower limit can be combined with other lower limits to form an unspecified range, and likewise any upper limit can be combined with any other upper limit to form an unspecified range. In addition, each separately disclosed point or single value can itself be combined as a lower limit or upper limit with any other point or single value or with other lower limits or upper limits to form an unspecified range.

[0050] The temperature parameters in this application, unless otherwise specified, allow for both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows the temperature to fluctuate within the accuracy range of instrument control.

[0051] In the description of the invention, “plurality” means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0052] If there is no special description, all embodiments and optional embodiments of the present application can be combined with each other to form a new technical solution. If there is no special description, all technical features and optional technical features of the present application can be combined with each other to form a new technical solution.

[0053] If there is no special explanation, all the steps of the present application can be performed sequentially or randomly, preferably sequentially.

[0054] The antioxidant capacity of the skin refers to the ability of the skin itself and the antioxidant ingredients obtained through the use of skin care products to jointly resist the oxidative stress caused by free radicals. Free radicals are one of the main causes of skin aging, pigmentation, inflammation and other problems. In order to fight these harmful factors, the skin has a defense system composed of endogenous antioxidant enzymes (such as catalase) and exogenous antioxidant substances (such as vitamin E and polyphenolic compounds). Among them, the DPPH free radical scavenging rate test is a simple and effective method for evaluating the antioxidant properties of a substance or product. Substances with high scavenging rates can more effectively neutralize free radicals, thereby helping to maintain the health of the skin and enhance its overall antioxidant level.

[0055] In the related art, antioxidant products can easily put the skin in a state of oxidative stress, resulting in skin problems such as skin inflammation, erythema, wrinkles, and acne.

[0056] Based on the above problems, the present application adopts the compound use of lipopeptides and yeast extracts, which can not only effectively remove free radicals, but also reduce the occurrence of skin stress and improve skin condition.

[0057] One or more embodiments of the present application provide a composition having the effect of scavenging free radicals, comprising lipopeptide, yeast extract and water; the mass proportion of lipopeptide in the composition is 0.001wt%-99wt%, and the mass proportion of yeast extract in the composition is 0.01wt%-30wt%.

[0058] As an example, the mass proportion of lipopeptide in the composition can be but is not limited to 0.001wt%, 0.005wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.5wt%, 1wt%, 5wt%, 10wt%, 15wt%, 20wt%, 25wt%, 30wt%, 35wt%, 40wt%, 45wt%, 50wt%, 55wt%, 60wt%, 65wt%, 70wt%, 75wt%, 80wt%, 85wt%, 90wt%, 95wt%, 99wt% or ranges between any two of the above mass proportions, etc.

[0059] The mass percentage of yeast extract in the composition may be, but is not limited to, 0.01 wt%, 0.05 wt%, 0.1 wt%, 0.5 wt%, 1 wt%, 5 wt%, 10 wt%, 15 wt%, 20 wt%, 25 wt%, 30 wt% or a range between any two of the foregoing mass percentages.

[0060] The main components of yeast extract include proteins, amino acids, peptides, nucleotides, vitamins and trace elements. Since yeast extract contains the above components, it can enhance the ability of lipopeptides to scavenge free radicals in the anti-oxidation process. In addition, yeast extract can also assist lipopeptides in neutralizing free radicals and reduce the oxidative damage of free radicals to cells. Furthermore, yeast extract is rich in glutathione and other functional components such as oligopeptides and amino acids. Glutathione helps to synthesize intracellular antioxidants in organisms, so yeast extract also has the effect of promoting the synthesis of intracellular antioxidants.

[0061] Understandably, the composition with free radical scavenging effect provided by the present application includes lipopeptide and yeast extract. Lipopeptide has the effect of scavenging free radicals and can eliminate the active oxygen generated in the body from the root; yeast extract and lipopeptide work synergistically to improve the scavenging rate of lipopeptide on free radicals, and can also promote the regeneration and repair of skin cells, enhance the barrier function of the skin, and improve the resistance of the skin; at the same time, yeast extract can also promote the metabolism of epidermal keratinocytes, making the skin delicate and elastic. Therefore, the composition with free radical scavenging effect can effectively reduce the occurrence of skin stress and improve the skin condition while effectively scavenging free radicals.

[0062] In some optional embodiments, the mass percentage of lipopeptide in the composition is 10wt%-50wt%.

[0063] As a possible implementation manner, the yeast extract accounts for 5wt%-20wt% of the composition.

[0064] In some embodiments, the lipopeptide is a cyclic or linear lipopeptide with a specific structure, having one of the structures of the following formula (I), (II) or (III).

[0065]

[0066] In the above structure, R represents the carbon chain of the fatty acid chain, and A1, A2, A3...Am represent the 1st, 2nd, 3rd...mth amino acids on the peptide chain respectively. The carboxyl group of the fatty acid is connected to the N-terminal of A1 (i.e. the first amino acid of the peptide chain), and at the same time, the C-terminal carboxyl group of Am (i.e. the last amino acid of the peptide chain) is connected to the hydroxyl or amino group of other amino acids on the fatty acid or peptide chain, thus forming a ring structure. When this cyclic lipopeptide is opened, it will become a linear product, that is, a linear lipopeptide.

[0067] In some embodiments, the lipopeptide includes one or more of surfactin, iturin, fenmucin, lichenin, babylonin, pamiramycin, sporomycin, mycosubtiline, phosphatidylinositol, fusaricide, kurstak, bacillus, polymyxin, octapeptidin, and peptidomycin.

[0068] In some optional embodiments, the lipopeptide is produced by bacteria. Further, the lipopeptide is produced by wild-type or genetically engineered bacteria. The bacteria are preferably selected from the following categories: Bacillus, Pseudomonas, Streptomyces and Arthrobacter. Among them, it is particularly preferred to produce the target lipopeptide by culturing Bacillus or Pseudomonas.

[0069] Regarding the selection of genes related to lipopeptide synthesis, the following can be selected: lipopeptide synthesis gene srfA, fenmustine synthesis gene fen, lichenin synthesis gene lic, spore mycin synthesis gene bam, mycobacterium myc, iturin synthesis gene itu, phospholipid synthesis gene pps, fusin, krs, polymyxin pmx, octapeptide mycin oct, transmembrane transport protein gene ycxA, biotin carboxylase gene yngH, spore formation related genes spoIVA / B / C / F, spoVA / B / D / E, and leuABCD / ilvK in the leucine biosynthesis pathway, etc. Preferably, it is produced by fermentation of Bacillus subtilis THY-7 / Pg3-srfA.

[0070] In some optional embodiments, the lipopeptide is produced by fermentation using Bacillus subtilis THY-7 / Pg3-srfA as a host strain.

[0071] In some embodiments, the composition having free radical scavenging efficacy further comprises a first moisturizer.

[0072] In some optional embodiments, the mass proportion of the first moisturizing agent in the composition is 0.001wt%-50wt%; for example, it can be but not limited to 0.001wt%, 0.005wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.5wt%, 1wt%, 5wt%, 10wt%, 15wt%, 20wt%, 25wt%, 30wt%, 35wt%, 40wt%, 45wt%, 50wt% or the range between any two of the above mass proportions, etc.

[0073] In some embodiments, the mass percentage of the first moisturizing agent in the composition is 0.5 wt %-10 wt %.

[0074] As a possible implementation, the first moisturizer includes one or more of trehalose, hydroxyethyl urea, mannan, sodium hyaluronate, bletilla striata polysaccharide, aloe polysaccharide, dendrobium officinale polysaccharide, glycerol polyoxyethylene ether, glycerol polyether-26 and polyoxyethylene-polyoxypropylene copolymer.

[0075] In some embodiments, the free radical scavenging composition further comprises a first emollient.

[0076] In some optional embodiments, the mass proportion of the first emollient in the composition is 0.001wt%-50wt%; for example, it can be but not limited to 0.001wt%, 0.005wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.5wt%, 1wt%, 5wt%, 10wt%, 15wt%, 20wt%, 25wt%, 30wt%, 35wt%, 40wt%, 45wt%, 50wt% or the range between any two of the above mass proportions, etc.

[0077] In some optional embodiments, the mass percentage of the first emollient in the composition is 1 wt %-10 wt %.

[0078] As a possible embodiment, the first emollient includes one or more of olive oil, shea butter, behenyl myristate, caprylate, oleyl oleate, hexyl laurate, isopropyl palmitate, coconut oil, sunflower oil, corn oil, rapeseed oil, mineral esters, mineral oil, beeswax, lanolin, liquid paraffin, squalene, PEG-6 caprylic / capric triglyceride and dioctylcyclohexane.

[0079] In some embodiments, the composition having free radical scavenging efficacy further comprises a first pH adjuster.

[0080] In some optional embodiments, the mass proportion of the first pH adjuster in the composition is 0.001wt%-20wt%; for example, it can be but not limited to 0.001wt%, 0.005wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.5wt%, 1wt%, 2wt%, 4wt%, 6wt%, 8wt%, 10wt%, 12wt%, 14wt%, 16wt%, 18wt%, 20wt% or the range between any two of the above mass proportions, etc.

[0081] In some embodiments, the first pH adjuster accounts for 0.01 wt%-5wt% of the composition.

[0082] As a possible implementation, the first pH adjuster includes one or more of citric acid, malic acid, lactic acid, sodium hydroxide, potassium hydroxide, serine, glutamic acid, disodium hydrogen phosphate and arginine.

[0083] In some embodiments, the composition having free radical scavenging efficacy further comprises a polyol.

[0084] In some optional embodiments, the mass proportion of the polyol in the composition is 0.001wt%-30wt%; for example, it can be but not limited to 0.001wt%, 0.005wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.5wt%, 1wt%, 2wt%, 4wt%, 6wt%, 8wt%, 10wt%, 12wt%, 14wt%, 16wt%, 18wt%, 20wt%, 22wt%, 24wt%, 26wt%, 28wt%, 30wt% or the range between any two of the above mass proportions, etc.

[0085] As a possible implementation, the polyol includes one or more of butylene glycol, propylene glycol, sorbitol, glycerol, pentylene glycol, polypropylene glycol and glycerol.

[0086] In some embodiments, the composition having free radical scavenging efficacy further comprises a first emulsifier.

[0087] In some optional embodiments, the mass proportion of the first emulsifier in the composition is 0.001wt%-50wt%; for example, it can be but not limited to 0.001wt%, 0.005wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.5wt%, 1wt%, 5wt%, 10wt%, 15wt%, 20wt%, 25wt%, 30wt%, 35wt%, 40wt%, 45wt%, 50wt% or the range between any two of the above mass proportions, etc.

[0088] In some embodiments, the mass percentage of the first emulsifier in the composition is 1 wt %-10 wt %.

[0089] As a possible embodiment, the first emulsifier includes one or more of sodium lauryl sulfate, potassium lauryl sulfate, octadecyltrimethylammonium bromide, PEG-100 stearate, steareth-2, steareth-21, glyceryl stearate, steareth-2, polyoxyethylene monostearate and lecithin.

[0090] In some embodiments, the composition having free radical scavenging efficacy further comprises a first preservative.

[0091] In some optional embodiments, the mass proportion of the first preservative in the composition is 0.001wt%-10wt%; for example, it can be but not limited to 0.001wt%, 0.005wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.5wt%, 1wt%, 2wt%, 3wt%, 4wt%, 5wt%, 6wt%, 7wt%, 8wt%, 9wt%, 10wt% or the range between any two of the above mass proportions, etc.

[0092] In some embodiments, the mass percentage of the first preservative in the composition is 0.01 wt %-1 wt %.

[0093] As a possible embodiment, the first preservative includes one or more of phenoxyethanol, p-hydroxyacetophenone, imidazolidinyl urea, diazolidinyl urea, sodium hydroxymethylglycinate, methylparaben, ethylparaben, propylparaben, potassium sorbate, methylisothiazolinone, DMDM ​​hydantoin, sodium benzoate and benzyl alcohol.

[0094] In some embodiments, the solvent having free radical scavenging efficacy comprises deionized water.

[0095] One or more embodiments of the present application provide a use of the above-mentioned composition with free radical scavenging efficacy in preparing a skin care product with antioxidant efficacy.

[0096] One or more embodiments of the present application provide a skin care product, comprising the above-mentioned composition having the effect of scavenging free radicals.

[0097] The skin care product comprises the above-mentioned composition with the function of removing free radicals and has the functions of anti-oxidation and anti-aging.

[0098] In some embodiments, the mass proportion of the composition in the skin care product is 0.1wt%-10wt%; for example, it can be but not limited to 0.1wt%, 0.3wt%, 0.5wt%, 1wt%, 1.5wt%, 2wt%, 2.5wt%, 3wt%, 3.5wt%, 4wt%, 4.5wt%, 5wt%, 5.5wt%, 6wt%, 6.5wt%, 7wt%, 7.5wt%, 8wt%, 8.5wt%, 9wt%, 9.5wt%, 10wt% or the range between any two of the above mass proportions, etc. When the mass proportion of the composition in the skin care product is within the above range, it has excellent antioxidant effect.

[0099] In some optional embodiments, the composition accounts for 0.5wt%-5wt% of the skin care product.

[0100] In some embodiments, based on 100 parts by mass of the skin care product, the skin care product includes: 0.1 to 10 parts of the composition, 10 to 20 parts of the second moisturizer, 8 to 15 parts of the second emollient, 0.5 to 3 parts of the second emulsifier, 0.02 to 0.06 parts of the second pH adjuster, 0.3 to 1 parts of the second preservative, 0.1 to 0.3 parts of the thickener, 0.01 to 0.05 parts of the chelating agent, 0.05 to 0.2 parts of the fragrance and 50 to 80 parts of deionized water.

[0101] It should be noted that "first moisturizer", "second moisturizer", "first emollient", "second emollient", "first emulsifier", "second emulsifier", "first pH adjuster", "second pH adjuster", "first preservative", "second preservative" and the like are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or quantity, nor can they be understood as implicitly indicating the importance or quantity of the indicated technical features. Moreover, "first", "second" and the like only serve the purpose of non-exhaustive enumeration and description, and it should be understood that they do not constitute a closed limitation on quantity.

[0102] As an example, the mass fraction of the composition with free radical scavenging effect contained in the skin care product can be but is not limited to 0.1 parts, 0.3 parts, 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts or the range between any two of the above mass fractions, etc.

[0103] The mass fraction of the second moisturizer contained in the skin care product may be, but is not limited to, 10 parts, 12 parts, 14 parts, 16 parts, 18 parts, 20 parts or a range between any two of the above mass fractions.

[0104] The mass fraction of the second lotion contained in the skin care product can be but is not limited to 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts or a range between any two of the above mass fractions.

[0105] The mass fraction of the second emulsifier contained in the skin care product can be, but is not limited to, 0.5 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, or a range between any two of the above mass fractions.

[0106] The mass fraction of the second pH adjuster contained in the skin care product may be, but is not limited to, 0.02 parts, 0.03 parts, 0.04 parts, 0.05 parts, 0.06 parts or a range between any two of the above mass fractions.

[0107] The mass fraction of the second preservative contained in the skin care product can be but is not limited to 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part or a range between any two of the above mass fractions.

[0108] The mass fraction of the thickener contained in the skin care product may be, but is not limited to, 0.1 part, 0.15 part, 0.2 part, 0.25 part, 0.3 part or a range between any two of the above mass fractions.

[0109] The mass fraction of the chelating agent contained in the skin care product may be, but is not limited to, 0.01 part, 0.02 part, 0.03 part, 0.04 part, 0.05 part or a range between any two of the above mass fractions.

[0110] The mass fraction of the fragrance contained in the skin care product can be, but is not limited to, 0.05 parts, 0.1 parts, 0.15 parts, 0.2 parts, or a range between any two of the above mass fractions.

[0111] The mass fraction of deionized water contained in the skin care product can be but is not limited to 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts or a range between any two of the above mass fractions.

[0112] In some embodiments, the second humectant includes one or more of glycerin and butylene glycol.

[0113] As a possible implementation, the second emollient includes one or more of isononyl isononanoate, dicaprylyl carbonate and polydimethylsiloxane.

[0114] In some alternative embodiments, the second emulsifier includes one or more of eicosylbehenyl alcohol and eicosyl glucoside.

[0115] In some exemplary embodiments, the second pH adjuster includes sodium hydroxide.

[0116] In some embodiments, the second preservative includes one or more of p-hydroxyacetophenone and caprylhydroxamic acid.

[0117] As a possible implementation, the thickener includes acrylic acid (esters) / C10-30 alkyl acrylate cross-polymer.

[0118] In some embodiments, the chelating agent comprises disodium EDTA.

[0119] In some embodiments, the pH value of the skin care product is 6.8-7.4; for example, it can be but not limited to 6.8, 6.9, 7, 7.1, 7.2, 7.3, 7.4 or a range between any two of the above pH values.

[0120] In some embodiments, the skin care product includes one or more of a lotion, an emulsion, a cream, a gel, an essence, a mask, a gel, and a spray.

[0121] The technical scheme of the present application is described in detail below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present application and are not used to limit the scope of the present application. The experimental methods for which specific conditions are not specified in the following embodiments are preferably referred to the guidance given in the present application, and can also be carried out according to the experimental manual or conventional conditions in the field, or according to the conditions recommended by the manufacturer, or with reference to experimental methods known in the art.

[0122] In the following specific embodiments, the measured parameters of raw material components may have slight deviations within the range of weighing accuracy unless otherwise specified. For temperature and time parameters, acceptable deviations caused by instrument test accuracy or operation accuracy are allowed.

[0123] It should be noted that iturin, fenmucin, lichenin, bacillin, kurstak, babilin, pamiram, spormycin, octapeptidin, mycosubtilin, lecithin, fusarin and polymyxin used in the following examples and comparative examples were all purchased from MedChemExpress. Yeast extract was purchased from Angel Yeast Co., Ltd.

[0124] The preparation method of the surfactin used in the following embodiments and comparative examples includes:

[0125] A strain was selected from a single colony of Bacillus subtilis THY 7 / Pg3 srfA (the characteristics of Bacillus subtilis THY 7 / Pg3 srfA are disclosed in the patent publication number CN109097315A), inoculated into LB liquid culture medium, and cultured at 37°C and 200 rpm for 16 hours to obtain a seed solution. Subsequently, the seed solution was transferred to a shake flask of a fermentation medium at a ratio of 5%, and continued to be cultured at the same temperature and speed. When the culture time reached 6 hours, 1 Mm IPTG was added thereto, and the culture was maintained for two days, and finally a fermentation broth containing surfactin was obtained.

[0126] The composition of the fermentation medium used was: 100 g / L sugars, 50 g / L inorganic nitrogen source, 3 g / L organic nitrogen source, 1 g / L KH2PO4, 0.3 g / L Na2HPO4·12H2O, 0.01 g / L CaCl2, 0.01 g / L MnSO4･H2O, 0.01 g / L FeSO 4･ 7H2O0.01g / L, pH 6.8.

[0127] The following method was used to determine the content of surfactin: First, 1 mL of sample was taken from the fermentation broth and centrifuged at 12,000 rpm for 1 minute. Then 100 μL was taken from the supernatant and mixed evenly with 1,900 μL of deionized water. After filtering through a 0.22 μm filter membrane, high performance liquid chromatography (HPLC) was used for analysis. In the HPLC analysis, the mobile phase consisted of methanol and water in a ratio of 85 / 15, and the flow rate was set to 1 mL / min. The chromatographic column used was a C18 ODS reverse phase column, the column temperature was maintained at 40 degrees Celsius, a UV detector was used, and detection was performed at a wavelength of 205 nm.

[0128] The test showed that the content of surfactin in the fermentation broth was 30%. After the surfactin was successfully extracted from the fermentation broth, it was converted into a powder form by freeze drying technology. It was then purified to finally obtain highly purified (purity reached 99.99wt%) surfactin for later use.

[0129] 1. Preparation of a composition having free radical scavenging effect

[0130] 1. Formula composition

[0131] The formulation compositions of Examples 1-30 and Comparative Examples 1-3 are shown in Table 1.

[0132] Table 1

[0133]

[0134] In Table 1, each "portion" represents 100 g.

[0135] 2. Preparation process

[0136] The lipopeptide, yeast extract and deionized water are mixed to prepare a composition.

[0137] 2. Performance test of the composition

[0138] 1. DPPH free radical scavenging ability determination

[0139] (1) Experimental principle: DPPH free radical scavenging experiment is a commonly used method for determining antioxidant capacity. DPPH is a stable free radical in organic solvents. Its alcohol solution is purple and needs to be stored at low temperature and away from light. In the presence of free radical scavengers, the single electron of DPPH is captured, making its color lighter and the absorbance at the wavelength of maximum light absorption decreases. The decrease in absorbance level indicates an increase in antioxidant activity, thereby evaluating the antioxidant capacity of the test sample. This antioxidant capacity is expressed by inhibition rate. The greater the inhibition rate, the stronger the antioxidant capacity.

[0140] (2) Experimental method: The compositions of Examples 1-30 and Comparative Examples 1-3 were respectively prepared into sample solutions with a mass percentage concentration of 0.4 wt% using water for determination. Take 0.5 mL of the sample solution, add 2 mL of a 60 μmol / L DPPH solution and mix well, let stand for 30 min, adjust the zero point with the original solvent, and measure the absorbance at 515 nm as Ai; in the same way, 0.5 mL of solvent + 2 mL of DPPH solution were mixed and measured to have an absorbance of Ac; 0.1 mL of the sample solution + 2 mL of solvent were mixed and measured to have an absorbance of Aj. The free radical scavenging rate was calculated according to the formula: scavenging rate (%) = [1-(Ai-Aj) / Ac] X100%.

[0141] The test results of the above embodiments and comparative examples are shown in Table 2.

[0142] Table 2

[0143]

[0144] From the results of Examples 1-30 in Table 2, it can be seen that the composition of the present application has the effect of scavenging DPPH free radicals.

[0145] From the comparison of the results of Examples 16-21 and Comparative Examples 1 and 3, it can be seen that compared with adding only lipopeptide to the composition, or compared with using other substances instead of yeast extract, adding lipopeptide and yeast extract to the composition at the same time, the DPPH free radical scavenging effect is significantly improved. In Comparative Example 2, only yeast extract is used without adding lipopeptide, and it does not have a DPPH free radical scavenging effect. Yeast extract mainly plays the role of improving the free radical scavenging rate of lipopeptide, promoting lipopeptide to neutralize the generated oxides, reducing their damage to cells, and maintaining the normal function of cells.

[0146] The results of Examples 1-15 show that, at the same ratio, different types of lipopeptides all have scavenging effects on DPPH free radicals. The results of Examples 16-27 show that when the mass proportion of lipopeptide in the composition is 10wt%-50wt% and the mass proportion of yeast extract in the composition is 5wt%-20wt%, the composition has a better DPPH free radical scavenging rate.

[0147] 2. ABTS free radical scavenging ability assay

[0148] (1) Experimental principle: ABTS reacts with oxidants (such as potassium persulfate K2S2O8) to generate stable blue-green cationic free radicals ABTS+·. This free radical has a maximum absorption wavelength at 734nm (or 405nm, but 734nm is more commonly used). When antioxidants are added to the ABTS+· solution, the antioxidant components will react with ABTS+·, causing the reaction system to fade, that is, the absorbance at 734nm decreases. This change in absorbance is proportional to the degree of free radical scavenging, so the ABTS free radical scavenging ability of antioxidants can be evaluated by measuring the change in absorbance.

[0149] Experimental method: a. Use water to prepare the compositions of Examples 1-30 and Comparative Examples 1-3 into sample solutions with a mass percentage concentration of 0.4wt% for measurement. Add 100μL of standard / sample solution to a 96-well plate, add 100μL of sample solvent to a negative control, and then add 100μL of ABTS solution. b. Add 100μL of standard / sample solution to a 96-well plate, add 100μL of sample solvent to a negative control, and then add 100μL of 95% ethanol (i.e. ABTS is replaced by the same volume of 95% ethanol) . Make 3 parallels for each sample.

[0150] The test results of the above embodiments and comparative examples are shown in Table 3.

[0151] Table 3

[0152]

[0153] From the results of Examples 1-30 in Table 3, it can be seen that the composition of the present application has the effect of scavenging ABTS free radicals.

[0154] From the comparison of the results of Examples 15-21 and Comparative Examples 1 and 3, it can be seen that compared with adding only lipopeptide to the composition, or compared with using other substances instead of yeast extract, adding lipopeptide and yeast extract to the composition at the same time, the ABTS free radical scavenging effect is significantly improved. In Comparative Example 2, only yeast extract is used without adding lipopeptide, and it does not have the effect of scavenging ABTS free radicals. Yeast extract mainly plays the role of improving the free radical scavenging rate of lipopeptide, promoting lipopeptide to neutralize the generated oxides, reducing their damage to cells, and maintaining the normal function of cells.

[0155] The results of Examples 1-15 show that, at the same ratio, different types of lipopeptides have scavenging effects on ABTS free radicals. The results of Examples 16-27 show that when the mass proportion of lipopeptide in the composition is 10wt%-50wt% and the mass proportion of yeast extract in the composition is 5wt%-20wt%, the composition has a better ABTS free radical scavenging rate.

[0156] It can be seen from the results in Table 2 and Table 3 that the composition of the present application has a strong scavenging effect on both DPPH free radicals and ABTS free radicals, that is, it has an excellent antioxidant effect.

[0157] 3. Preparation of skin care lotion

[0158] It should be noted that each “portion” in the following application examples and comparative application examples refers to 100 g.

[0159] Application Example 1

[0160] 1. Formula composition

[0161] The aqueous phase includes: 10 parts of glycerin, 5 parts of butylene glycol, 0.05 parts of disodium EDTA, 0.2 parts of p-hydroxyacetophenone and 72.56 parts of deionized water; the oil phase includes: 6 parts of isononyl isononanoate, 0.5 parts of the composition in Example 19, 3 parts of dicaprylyl carbonate, 1 part of polydimethylsiloxane and 1 part of a mixture of arachidyl behenyl alcohol and arachidyl glucoside; the additive phase includes: 0.15 parts of acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, 0.04 parts of sodium hydroxide, 0.4 parts of capryl hydroxamic acid and 0.1 parts of rose essence. Among them, the mixture of arachidyl behenyl alcohol and arachidyl glucoside is purchased from Seppic, and the trade name is MONTANOV202.

[0162] 2. Preparation process

[0163] Step 1, in an oil phase pretreatment tank, put the oil phase material into a kettle and heat it to 92°C, while starting the stirrer until it is completely melted to obtain an oil phase mixture;

[0164] Step 2: In the water phase pretreatment tank, accurately weigh the water phase material and put it into the kettle and heat it to 92° C., while starting the agitator until it is completely dissolved to obtain a water phase mixture;

[0165] Step 3, the oil phase mixture filtered through the pipeline is sucked into the emulsification tank preheated to 90°C, and then the water phase mixture is sucked, and the scraper stirring, rapid stirring and homogenizer are started in sequence, and the mixture is stirred for 10 minutes at a temperature preservation temperature, and then the cooling device is started to start cooling, and the temperature in the tank is reduced to 80°C;

[0166] Step 4. When the temperature drops below 80°C, take the material for pH value testing. When the paste temperature drops to 55°C, add the components in the added phase, stir well, and then continue to cool to 35°C. Add sodium hydroxide to adjust the pH value to obtain an antioxidant skin care lotion.

[0167] Application Example 2

[0168] The preparation methods of Application Example 2 and Application Example 1 are similar, except that in Application Example 2, 0.5 parts of the composition in Example 1 are used to replace the composition in Example 19, and the rest are the same.

[0169] Application Example 3

[0170] The preparation methods of Application Example 3 and Application Example 1 are similar, except that in Application Example 3, 0.5 parts of the composition in Example 28 are used to replace the composition in Example 19, and the rest are the same.

[0171] Application Example 4

[0172] The preparation methods of Application Example 4 are similar to those of Application Example 1, except that 0.1 parts of the composition in Example 19 is used in Application Example 4.

[0173] Application Example 5

[0174] The preparation methods of Application Example 5 are similar to those of Application Example 1, except that 5 portions of the composition in Example 19 are used in Application Example 5.

[0175] Application Example 6

[0176] The preparation methods of Application Example 6 are similar to those of Application Example 1, except that 10 portions of the composition in Example 19 are used in Application Example 6.

[0177] Application Comparative Example 1

[0178] The preparation methods of Application Comparative Example 1 and Application Example 1 are similar, except that: in Application Comparative Example 1, 0.5 parts of the composition in Comparative Example 1 are used to replace the composition in Example 19, and the rest are the same.

[0179] Application Comparative Example 2

[0180] The preparation methods of Application Example 2 are similar to those of Application Example 1, except that in Application Example 2, 0.5 parts of the composition in Application Example 2 are used to replace the composition in Example 19, and the rest are the same.

[0181] Application Comparative Example 3

[0182] The preparation methods of Application Example 3 and Application Example 1 are similar, except that: in Application Example 3, 0.5 parts of the composition in Application Example 3 are used to replace the composition in Example 19, and the rest are the same.

[0183] Application Comparative Example 4

[0184] The preparation methods of Application Comparative Example 4 and Application Example 1 are similar, except that 0.5 parts of deionized water are used to replace the composition in Example 19 in Application Comparative Example 4, and the rest are the same.

[0185] Application Comparative Example 5

[0186] The preparation methods of Application Comparative Example 5 and Application Example 1 are similar, except that 0.5 parts of tocopherol acetate is used to replace the composition in Example 19 in Application Comparative Example 5, and the rest are the same.

[0187] 4. Skin care lotion performance determination

[0188] 1. DPPH free radical scavenging rate test

[0189] The experimental principle and experimental method are the same as those described in "II. Performance test of the composition". The test results of application examples 1-6 and comparative application examples 1-5 are shown in Table 4.

[0190] Table 4

[0191]

[0192] 2. ABTS free radical scavenging rate test

[0193] The experimental principle and experimental method are the same as those described in "II. Performance test of the composition". The test results of application examples 1-6 and comparative application examples 1-5 are shown in Table 5.

[0194] Table 5

[0195]

[0196] It can be seen from the results in Table 4 and Table 5, and from the comparison of the results of Application Examples 1-6 and Application Comparative Examples 1-5, it can be seen that the skin care lotion provided in the present application has excellent DPPH free radical scavenging efficacy and ABST free radical scavenging efficacy, that is, it has excellent antioxidant properties.

[0197] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0198] The above-mentioned embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the attached claims.

Claims

1. A composition having the effect of scavenging free radicals, characterized in that: The composition comprises lipopeptide, yeast extract and water; the mass proportion of the lipopeptide in the composition is 0.001wt%-99wt%, and the mass proportion of the yeast extract in the composition is 0.01wt%-30wt%.

2. The composition according to claim 1, characterized in that The mass proportion of the lipopeptide in the composition is 10wt%-50wt%; and / or The yeast extract accounts for 5wt%-20wt% of the composition.

3. The composition according to claim 1, characterized in that The lipopeptide comprises one or more of surfactin, iturin, fenmucin, lichenin, babylonin, pamiramycin, sporemycin, mycobacterium, phospholipid inhibitor, fusaricide, kurstak, bacillus, polymyxin, octapeptide and polypeptide.

4. The composition according to any one of claims 1 to 3, characterized in that The composition has at least one of the following characteristics (1)-(6): (1) The composition further comprises a first moisturizer; Optionally, the mass proportion of the first moisturizing agent in the composition is 0.001wt%-50wt%, and optionally 0.5wt%-10wt%; Optionally, the first moisturizing agent includes one or more of trehalose, hydroxyethyl urea, mannan, sodium hyaluronate, bletilla striata polysaccharide, aloe polysaccharide, dendrobium officinale polysaccharide, glycerol polyoxyethylene ether, glycerol polyether-26 and polyoxyethylene-polyoxypropylene copolymer; (2) The composition further comprises a first emollient; Optionally, the mass proportion of the first emollient in the composition is 0.001wt%-50wt%, optionally 1wt%-10wt%; Optionally, the first emollient comprises one or more of olive oil, shea butter, behenyl myristate, caprylate, oleyl oleate, hexyl laurate, isopropyl palmitate, coconut oil, sunflower oil, corn oil, rapeseed oil, mineral esters, mineral oil, beeswax, lanolin, liquid paraffin, squalene, PEG-6 caprylic / capric triglyceride and dioctylcyclohexane; (3) The composition further comprises a first pH adjusting agent; Optionally, the mass proportion of the first pH adjuster in the composition is 0.001 wt%-20 wt%, and optionally 0.01 wt%-5 wt%; Optionally, the first pH adjuster includes one or more of citric acid, malic acid, lactic acid, sodium hydroxide, potassium hydroxide, serine, glutamic acid, disodium hydrogen phosphate and arginine; (4) The composition further comprises a polyol; Optionally, the mass proportion of the polyol in the composition is 0.001wt%-30wt%; Optionally, the polyol includes one or more of butylene glycol, propylene glycol, sorbitol, glycerol, pentylene glycol, polypropylene glycol and glycerol; (5) The composition further comprises a first emulsifier; Optionally, the mass proportion of the first emulsifier in the composition is 0.001wt%-50wt%, optionally 1wt%-10wt%; Optionally, the first emulsifier includes one or more of sodium lauryl sulfate, potassium lauryl sulfate, octadecyl trimethyl ammonium bromide, PEG-100 stearate, steareth-2, steareth-21, glyceryl stearate, steareth-2, polyoxyethylene monostearate and lecithin; (6) The composition further comprises a first preservative; Optionally, the mass proportion of the first preservative in the composition is 0.001wt%-10wt%, optionally 0.01wt%-1wt%; Optionally, the first preservative includes one or more of phenoxyethanol, p-hydroxyacetophenone, imidazolidinyl urea, diazolidinyl urea, sodium hydroxymethylglycinate, methylparaben, ethylparaben, propylparaben, potassium sorbate, methylisothiazolinone, DMDM ​​hydantoin, sodium benzoate and benzyl alcohol.

5. Use of the composition having free radical scavenging effect as claimed in any one of claims 1 to 4 in the preparation of a skin care product having antioxidant effect.

6. A skin care product, characterized in that: The invention comprises a composition having free radical scavenging effect as claimed in any one of claims 1 to 4.

7. The skin care product according to claim 6, characterized in that The mass proportion of the composition in the skin care product is 0.1wt%-10wt%, and can be optionally 0.5wt%-5wt%.

8. The skin care product according to any one of claims 6 to 7, characterized in that: Taking the mass parts of the skin care product as 100 parts, the skin care product comprises: 0.1 to 10 parts of the composition, 10 to 20 parts of a second moisturizer, 8 to 15 parts of a second emollient, 0.5 to 3 parts of a second emulsifier, 0.02 to 0.06 parts of a second pH adjuster, 0.3 to 1 part of a second preservative, 0.1 to 0.3 parts of a thickener, 0.01 to 0.05 parts of a chelating agent, 0.05 to 0.2 parts of a fragrance, and 50 to 80 parts of deionized water.

9. The skin care product according to claim 8, characterized in that The skin care product has at least one of the following characteristics (1)-(7): (1) the second humectant comprises one or more of glycerin and butylene glycol; and / or (2) the second emollient comprises one or more of isononyl isononanoate, dicaprylyl carbonate and polydimethylsiloxane; (3) the second emulsifier includes one or more of arachidyl docosyl alcohol and arachidyl glucoside; (4) the second pH adjuster includes sodium hydroxide; (5) the second preservative comprises one or more of p-hydroxyacetophenone and caprylhydroxamic acid; (6) The thickener includes acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer; (7) The chelating agent includes disodium EDTA; (8) The pH value of the skin care product is 6.8-7.

4.

10. The skin care product according to claim 6, characterized in that: The skin care products include one or more of lotions, emulsions, creams, gels, essences, facial masks, gels and sprays.

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