Anti-inflammatory soothing composition for repairing red acne marks and its application, cosmetics and skin care products

Abstract

The present invention discloses an anti-inflammatory soothing composition for repairing red acne marks, which comprises the following components in parts by weight: 0.01-1 parts of kava pepper extract, 0.05-1 parts of powdered tetrandra extract, 0.01-2 parts of citrus fruit extract, 0.01-0.5 parts of dipotassium glycyrrhizinate, 0.01-0.5 parts of allantoin, and 0.01-1 parts of hydrolyzed red algae extract. The present invention also provides an application, cosmetics and skin care products of the anti-inflammatory soothing composition. The anti-inflammatory soothing composition for repairing red acne marks of the present invention is mainly composed of kava pepper extract, powdered tetrandra extract, citrus fruit extract, dipotassium glycyrrhizinate, allantoin and hydrolyzed red algae extract, and a soothing composition for repairing red acne marks is prepared, the composition has significant efficacy, and the mutual synergistic effect between the components can effectively inhibit the occurrence of inflammatory acne and relieve acne red acne marks, providing assistance for the recovery of acne skin.

Description

Technical Field

[0001] The invention belongs to the field of skin care products, and in particular relates to an anti-inflammatory soothing composition and application thereof, cosmetics and skin care products. Background Art

[0002] Acne is a common chronic inflammatory skin disease that occurs in the pilosebaceous gland unit. Its skin lesions are widespread, mostly distributed on the forehead, cheeks and mandible, and can also affect the trunk, such as the front chest, back and shoulder blades. The manifestations of acne inflammatory skin lesions are mainly acne, papules and pustules. In severe cases, it can also cause nodules, cysts, scars, intracranial infections and other hazards. Acne is a disfiguring skin disease that often occurs in adolescents and has a great impact on their physical, psychological and social confidence.

[0003] The pathogenesis of acne has not yet been fully elucidated, but inflammation is involved in an important pathological process in the development of acne. The main pathological process of acne is the production of proinflammatory factors at the site of inflammation. Proinflammatory factors have chemotactic effects, causing inflammatory cells to accumulate in the inflammatory lesions and release inflammatory factors, leading to an inflammatory reaction. The red acne marks after acne may be due to the inflammatory reaction of acne causing capillary dilation and endothelial cell damage, forming red acne marks.

[0004] Prostaglandin E2 (PGE2) is the main factor in inducing inflammatory response and promoting vasodilation, while tumor necrosis factor TNF-α can mediate the production of vascular endothelial growth factor VEGF, which is recognized to promote the proliferation of capillary endothelial cells. In addition, interleukin-8 (IL-8) can also lead to increased vascular permeability, further aggravating the formation of red acne marks. In addition, the increase in the proportion of modern people staying up late and the change in the rhythm of life lead to the expression of other inflammatory factors in the skin, making the skin in an inflammatory state, further aggravating the generation and continuation of red acne marks. Therefore, how to regulate the inflammatory state of acne skin, especially when acne has already occurred, quickly and effectively reduce the level of skin inflammation, soothe the skin, and reduce the generation of red acne marks from the source, is an important issue that needs to be solved at present.

[0005] At present, the removal of acne scars is mostly done through traditional whitening methods such as reducing tyrosinase activity, inhibiting melanin production, and accelerating stratum corneum exfoliation. There is no particularly clear solution for red acne scars caused by inflammation. In fact, many acne scar removal products do not distinguish between red acne scars and black acne scars, nor do they provide reasonable solutions based on the mechanism. Summary of the invention

[0006] The technical problem to be solved by the present invention is to overcome the deficiencies and defects mentioned in the above background technology and provide an anti-inflammatory soothing composition for repairing red acne marks and its application, cosmetics and skin care products.

[0007] In order to solve the above technical problems, the technical solution proposed by the present invention is: An anti-inflammatory and soothing composition for repairing red acne marks, comprising the following components in parts by weight: Kava extract 0.01-1 part; 0.05-1 part of Stephania tetrandra extract; Citrus fruit extract 0.01-2 parts; Dipotassium glycyrrhizinate 0.01-0.5 parts; Allantoin 0.01-1 part; Hydrolyzed red algae extract 0.01-1 part.

[0008] The above anti-inflammatory soothing composition preferably comprises the following components in parts by weight: Kava extract 0.1-0.6 parts; 0.1-0.6 parts of Stephania tetrandra extract; Citrus fruit extract 0.1-1.5 parts; Dipotassium glycyrrhizinate 0.1-0.25 parts; Allantoin 0.05-0.25 parts; Hydrolyzed red algae extract 0.05-0.5 parts.

[0009] In the above anti-inflammatory and soothing composition, the weight ratio of the citrus fruit extract to the total weight of the Piper methysticum chuanxiong extract and the Tetrandrakia tetrandra extract is not less than 0.8.

[0010] In the present invention, kava extract, powdered tuber extract, citrus fruit extract, dipotassium glycyrrhizinate, allantoin and hydrolyzed red algae extract have obvious synergistic effects, which can quickly inhibit the generation of inflammatory acne, and can also relieve post-acne erythema caused by inflammation, promote the disappearance of acne and skin repair. Our further research shows that among the above components, considering that the medicinal mechanisms between the components may be related or mutually exclusive, the dosage between citrus fruit extract, kava extract and powdered tuber extract needs to be reasonably controlled to ensure the synergistic effect of each component. Our test results show that if the amount of citrus fruit extract is too small, the weight ratio of the weight of citrus fruit extract to the total weight of kava extract and powdered tuber extract is less than 0.8, the anti-inflammatory effect has a significant downward trend, and the repair effect of red acne marks will also be weakened.

[0011] In the above anti-inflammatory soothing composition, preferably, the extraction method of the Piper methysticum extract comprises the following steps: crushing Piper methysticum to obtain Piper methysticum powder, adding the powder to a polyol solution, stirring and dispersing the powder evenly, performing high-pressure homogenization, performing ultrasonic treatment, and then performing extraction treatment at room temperature, and obtaining the Piper methysticum extract after solid-liquid separation.

[0012] In the above anti-inflammatory soothing composition, preferably, the polyol solution is one or more of propylene glycol, butylene glycol, hexylene glycol and pentylene glycol, the mass content of the polyol in the polyol solution is 20-80%, and the mass content of gum arabic not exceeding 10% is further added to the polyol solution, and the mass concentration of the kava pepper in the polyol solution is 5-10%.

[0013] In the above anti-inflammatory soothing composition, preferably, the homogenization time during high-pressure homogenization treatment is 3-5 minutes, the homogenization pressure is 200-400 Bar, and the homogenization is repeated multiple times; the ultrasonic time during ultrasonic treatment is 30-60 minutes, and the ultrasonic temperature is 30-40°C; the single extraction time during extraction treatment is 20-24 hours, and the extraction is repeated multiple times.

[0014] The extraction method of the kava pepper extract uses a one-step method to prepare the kava pepper extract, does not require the use of alcohol or organic solvents, and chooses to use polyols, which are more gentle and friendly to the skin. In addition, the extraction process does not involve high-temperature treatment, which avoids the denaturation of the active ingredients due to high temperature and affects the efficacy. In addition, the addition of gum arabic can increase the emulsification properties of the extract, increase the dissolution of the active ingredients, and cooperate with the high-pressure homogenization wall breaking treatment and ultrasonic treatment, which is more conducive to the precipitation of the active ingredients. The organic combination of the above-mentioned multiple process means will improve the extraction efficiency of the active ingredients.

[0015] As a general technical concept, the present invention also provides an application of the above-mentioned anti-inflammatory and soothing composition in skin care products.

[0016] As a general technical concept, the present invention also provides a skin care product, the components of which include the above-mentioned anti-inflammatory and soothing composition.

[0017] As a general technical concept, the present invention also provides an application of the above anti-inflammatory and soothing composition in cosmetics.

[0018] As a general technical concept, the present invention also provides a cosmetic, the components of which include the above-mentioned anti-inflammatory and soothing composition.

[0019] Our research shows that acne is strongly associated with inflammation, and inflammatory factors interleukins (IL-1, IL-6, IL-8) and tumor necrosis factor TNF-α play an important role in the pathogenesis of acne. Therefore, in the process of treating acne, the inhibition of inflammatory factors is an indispensable matter. In addition, the sequelae of acne caused by inflammation are mainly post-acne erythema (PAE), which is typically manifested as red macules and capillary dilation, which is more obvious on light-colored skin. Although its pathogenesis is unknown, previous studies have shown that this may be related to the formation of capillary neoplasia, dilation and collagen tissue during inflammation. Post-acne erythema may slowly improve over time, but the erythema of most patients may last for several years or even longer. Therefore, based on the pathogenesis of inflammatory acne, the formation mechanism of inflammation on post-acne erythema, and the analysis of the mechanism of action of clinical treatment schemes, it can be seen that inhibiting skin inflammation is the key point to alleviate skin acne problems and inhibit the formation of post-acne erythema. Based on this, the present invention uses kava pepper extract, powdered tetrandra extract, citrus fruit extract, dipotassium glycyrrhizinate, allantoin and hydrolyzed red algae extract as main ingredients to prepare a soothing composition for repairing red acne marks. The composition can inhibit the expression of interleukin IL-8 and tumor necrosis factor TNF-α, can effectively reduce the expression of skin inflammatory factors, and reduce the proliferation of skin capillaries. The synergistic effect of the two can not only quickly inhibit the occurrence of inflammatory acne, but also relieve post-acne erythema caused by inflammation, promote the regression of acne and skin repair. The composition has significant efficacy, and the mutual synergistic effect between the components can effectively inhibit the occurrence of inflammatory acne and relieve acne red acne marks, providing assistance for the recovery of acne skin.

[0020] In the present invention, the mechanism of action and target of each component are specifically analyzed as follows: 1. Kava Extract Kava, commonly known as kava, is a perennial shrub of the Piperaceae family that grows wild in South Pacific islands such as Vanuatu, Fiji, Tonga, Papua-New Guinea and the Solomon Islands. Wild or cultivated, the roots and rhizomes are used as medicine, and the fresh leaves can also be used as medicine. This plant has always been a daily necessity for the residents of these island countries, used to relax the body and emotions, restore physical strength and relieve pain. In recent years, it has begun to be accepted by Europeans and Americans and has become one of the best-selling herbs in the United States and Germany.

[0021] The main active ingredients of kava are kavalactones, including capsaicin, dihydrocapsaicin, methoxycapsaicin and demethoxycapsaicin. In addition, certain kavalactones in kava are essential for the soothing effect of the raw material. Kava extract has the triple effect of instant sedation, anti-inflammatory and antibacterial. GABA, as one of the important inhibitory neurotransmitters, can inhibit the transmission of pain signals in the pain modulation pathway. Studies have shown that the active ingredients in kava can regulate neurotransmitters in a bidirectional manner, mediating the sedative effect in the body by affecting the binding of GABA to GABAa receptors. Kava extract acts on nerve endings, blocking the transmission of information by neurons through capsaicin occupying GABAa receptors and inhibiting PGE2 (PGE2 is a known mediator of inflammation that can cause pain), which may inhibit the transmission of sensitivity.

[0022] The extraction method of Kava pepper can be as follows: preferably, the roots, stems and leaves of Kava pepper are washed, sliced ​​and dried, crushed by a medicinal material crusher, and the medicinal material powder of suitable size is screened out through a sieve for standby use. A certain amount of polyol (one or more of propylene glycol, butylene glycol, hexylene glycol and pentanediol) is weighed and dispersed in water, and the content of polyol is 20-80%. In order to further increase the extraction efficiency and increase the stability of the solution, 0-10% gum arabic can be added to the extract. After the extract is prepared, the measured Kava pepper powder is added at a mass fraction of 5-10%, stirred and dispersed evenly, and then treated with high-pressure homogenization, the homogenization time is 3-5min, the homogenization pressure is 200-400Bar, and the material body is homogenized 3 times. After completing the high-pressure homogenization, the material body is placed in an ultrasonic device for ultrasonic treatment, the ultrasonic time is 30-60min, and the ultrasonic temperature is between 30-40°C. After ultrasonic treatment, the mixture was extracted at room temperature for 24 hours, and the kava extract was obtained after solid-liquid separation. In order to improve the utilization rate, all the kava extracts were extracted twice.

[0023] 2. Stephania tetrandra Extract Powdered Stephania tetrandra, also known as Hanfangji and Baimuxiang, is the root of the plant of the Menispermaceae family. It is dug up in autumn, the rough skin is removed, it is dried until half dry, cut into sections or cut longitudinally, and dried. It is mainly used to treat rheumatic arthralgia, especially heat arthralgia. It grows in mountainous hills, grass or the edge of dwarf forests. It is mainly produced in Jiangxi and Hunan, and also in Zhejiang, Anhui, Hubei and other provinces.

[0024] There have been relatively many studies on the chemical components of Stephania tetrandra, among which alkaloids are rich in content and have significant pharmacological activity. They are the active ingredients that are currently studied more. They are mainly dibenzylisoquinoline alkaloids, such as fangchinoline, fangchinoline, cephalothin, berberine, Norfangchinoline, Cycleanorine, etc., and aporphenanthrene alkaloids, such as bicuculline, dehydrobicuculline, dehydroclebanine, and arrhenomyridin. These alkaloids have a wide range of biological activities. For example, fangchinoline and bicuculline have good anti-inflammatory effects. Cephalothin has a good immunomodulatory effect. Fangchinoline and cephalothin can play an anti-neuroinflammatory role by inhibiting the release of IL-6, IL-1β and TNF-α.

[0025] 3. Citrus fruit extract Green peel is the peel of dried young or immature fruits of the Rutaceae plant citrus and its cultivated varieties. The 2020 edition of the Chinese Pharmacopoeia divides green peel into two different specifications: "individual green peel" and "four-flower green peel". The young fruits that fall naturally in May and June are collected and dried, commonly known as "individual green peel"; the immature fruits picked in July and August are cut into 4 petals on the peel to the base, the pulp is cut off, and after drying, they are called "four-flower green peel". Individual green peel is a widely used, safe and effective drug in clinical practice. It has the effects of soothing the liver, breaking up qi, dispersing knots and eliminating phlegm. It is mainly used to treat chest, rib and stomach pain, hernia, food accumulation, breast swelling, breast nodules, long-term malarial masses and other diseases. Individual green peel mainly contains volatile oils, flavonoids and alkaloids. Among them, the flavonoids are mainly hesperidin, naringin, neohesperidin, etc., which have anti-inflammatory, antibacterial and antiviral effects.

[0026] Hesperidin has anti-inflammatory, antioxidant, and microcirculation-improving effects. In animal models, hesperidin can reduce inflammatory responses and the pain responses caused by it by reducing the levels of inflammatory cytokines and anti-oxidation. At the same time, hesperidin has been shown to have anti-oxidative stress and anti-inflammatory effects in a mouse model of skin damage caused by ultraviolet radiation, mainly by downregulating the inflammatory cytokines secreted by the body, including TNF-α, IL-1β, IL-6, etc.

[0027] 4. Dipotassium glycyrrhizinate Dipotassium glycyrrhizinate is a derivative of glycyrrhizic acid, which is the main active ingredient of licorice. Licorice, also known as sweet root, is a herbaceous plant of the Leguminosae family, a commonly used Chinese medicinal material. Its medicinal parts are the roots and the tuberous stems underneath. According to literature records, licorice was listed as a top-grade product in medical monographs as early as the Eastern Han Dynasty two thousand years ago. It is "sweet in taste, mild in nature, and non-toxic", and can reduce swelling and detoxify. As a derivative of glycyrrhizic acid, dipotassium glycyrrhizinate has anti-inflammatory, anti-allergic, and antibacterial effects. It can effectively remove superoxide ions and hydroxyl free radicals, thereby achieving anti-inflammatory and antibacterial effects. In addition, dipotassium glycyrrhizinate has a good anti-allergic effect, can inhibit the production of histamine and skin redness and itching, and has a significant protective effect on certain allergic shocks.

[0028] 5. Allantoin Allantoin, also known as N-(2,5-dioxo-4-imidazolinyl)urea, has a molecular formula of C4H6N4O3 and is a hydantoin derivative. Allantoin is mainly used in the three fields of medicine, cosmetics and agriculture. Since allantoin is an amphoteric compound that can combine with a variety of substances to form complex salts, it has the effects of light protection, bactericidal preservation, analgesia and antioxidant. It can keep the skin hydrated, moisturized and soft. It is a special additive for cosmetics such as beauty and hairdressing. It is widely used as an additive for freckle cream, acne solution, shampoo, soap, toothpaste, shaving lotion, hair conditioner, astringent, antiperspirant and deodorant lotion. Allantoin can promote tissue growth, cell metabolism and soften stratum corneum protein. Allantoin can promote the absorption and retention of water in skin cells, thereby playing a moisturizing role and improving dry skin. Allantoin can promote the proliferation and differentiation of epidermal cells, help the stratum corneum to renew, and make the skin smoother and more delicate. In addition, allantoin has a certain antibacterial effect, and can promote cell growth and repair, and accelerate wound healing. In this composition, allantoin can not only soothe the skin, replenish moisture and moisturize, but also promote cell growth and repair of acne skin, and promote the renewal of the stratum corneum, providing a promoting effect for repairing red acne marks and improving skin condition.

[0029] 6. Hydrolyzed red algae extract Dendrobium officinale is a red algae that gets its color from an excess of red pigment (phycoerythrin), which expands the light energy absorption spectrum, allowing it to live in the deepest parts of the ocean. As an epiphytic seaweed, Dendrobium officinale is composed of cell filaments and lives with its host. Due to its small size, this seaweed is little known and rarely studied. However, it plays a significant role in helping the host resist external aggressions and providing a protective barrier. With the help of modern technology, hydrolyzed red algae extracts can be obtained by culturing in a photobioreactor, harvesting and separating the seaweed in cellular form, and then through a hydrolysis extraction process.

[0030] It should be emphasized that the above-mentioned groups do not play a role alone in the present invention, but play a synergistic role after the groups are combined. The synergistic effect of the components can effectively inhibit the occurrence of inflammatory acne and relieve acne redness and scars, providing assistance for the recovery of acne skin.

[0031] Compared with the prior art, the advantages of the present invention are: The anti-inflammatory soothing composition for repairing red acne marks of the present invention is mainly composed of kava pepper extract, powdered tetrandra extract, citrus fruit extract, dipotassium glycyrrhizinate, allantoin and hydrolyzed red algae extract, and a soothing composition for repairing red acne marks is prepared. The synergistic effect of the components in the composition can inhibit the expression of interleukin IL-8 and tumor necrosis factor TNF-α, can effectively reduce the expression of skin inflammatory factors, and reduce the proliferation of skin capillaries. The synergistic effect of the two can not only quickly inhibit the occurrence of inflammatory acne, but also relieve post-acne erythema caused by inflammation, promote the regression of acne and skin repair. The composition has significant efficacy, and the mutual synergistic effect between the components can effectively inhibit the occurrence of inflammatory acne and relieve acne red acne marks, providing assistance for the recovery of acne skin.

[0032] The anti-inflammatory and soothing composition for repairing red acne marks of the present invention has simple ingredients, clear action mechanism, and significant efficacy, and can be safely used in cosmetics and skin care products. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0034] Figure 1 This is a graph showing the inhibition rate of the anti-inflammatory and soothing composition on HaCaT cell TNF-α.

[0035] Figure 2 This is a graph showing the inhibition rate of the anti-inflammatory soothing composition on IL-8 in HaCaT cells in the examples.

[0036] Figure 3 This is a graph showing the change rate of the skin a* value after the composition of the embodiment and the comparative example is used on people with acne.

[0037] Figure 4 For some subjects, visia took pictures during the use of samples. DETAILED DESCRIPTION

[0038] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively and meticulously below in conjunction with the accompanying drawings and preferred embodiments of the present invention, but the protection scope of the present invention is not limited to the following specific embodiments.

[0039] Unless otherwise defined, all the professional terms used below have the same meanings as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0040] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0041] Embodiment 1: An anti-inflammatory soothing composition for repairing red acne marks, comprising kava extract, stephania tetrandra extract, citrus fruit extract, dipotassium glycyrrhizinate, allantoin and hydrolyzed red algae extract, and the weight ratio of each component is shown in Table 1 below.

[0042] Table 1: Weight ratio of the composition in the example (unit: g)

[0043] Among the above components, the extraction method of kava pepper can be as follows: kava pepper roots, stems, and leaves are washed, sliced, and dried, crushed with a medicinal material crusher, and the medicinal material powder of suitable size is screened out through a sieve for standby use. A certain amount of butanediol and pentanediol are weighed and dispersed in water, and the content of polyols is 20-80%. In order to further increase the extraction efficiency and increase the stability of the solution, 1% gum arabic can be added to the extract. After the extract is configured, the measured kava pepper root powder is added at a mass fraction of 10%, stirred and dispersed evenly, and then treated with high-pressure homogenization, the homogenization time is 5min, the homogenization pressure is 400Bar, and the material body is homogenized 3 times. After completing the high-pressure homogenization, the material body is placed in an ultrasonic device for ultrasonic treatment, the ultrasonic time is 60min, and the ultrasonic temperature is 40°C. After ultrasonic treatment, it is extracted at room temperature for 24h, and the kava pepper extract is obtained after solid-liquid separation. In order to improve the utilization rate, all kava peppers are extracted twice.

[0044] The above-mentioned Stephania tetrandra extract, citrus fruit extract, dipotassium glycyrrhizinate, allantoin and hydrolyzed red algae extract are purchased products, among which the Stephania tetrandra extract was purchased from Gansu Panzhi Pharmaceutical Co., Ltd. and / or Shanghai Guzidi Industrial Co., Ltd., the citrus fruit extract was purchased from Shanghai Kasyapa Industrial Co., Ltd., dipotassium glycyrrhizinate was purchased from Gansu Panzhi Pharmaceutical Co., Ltd., allantoin was purchased from Guangdong Wanshun Daily Chemical Co., Ltd., and the hydrolyzed red algae extract was purchased from Shanghai Faenkai Industrial Co., Ltd. and / or Guangzhou Baihaobo Co., Ltd.

[0045] When preparing the anti-inflammatory soothing composition, kava extract, stephania tetrandra extract, citrus fruit extract, dipotassium glycyrrhizinate, allantoin and hydrolyzed red algae extract are weighed and dissolved in deionized water. After mixing evenly, the mixture is filtered through a 0.22 μm inorganic membrane to obtain the composition.

[0046] Embodiment 2-6: The anti-inflammatory and soothing compositions in Examples 2-6 are different from those in Example 1 mainly in the different ratios of ingredients, as shown in Table 1 above.

[0047] Comparative Examples 1-6: The anti-inflammatory and soothing compositions in Comparative Examples 1-6 are different from those in Example 1 mainly in terms of ingredients and proportions, as shown in Table 1 above.

[0048] The effects of the anti-inflammatory and soothing compositions in Examples 1-6 and Comparative Examples 1-6 were verified as follows: ① Anti-inflammatory effect of the composition on immortalized keratinocytes - TNF-α: 1) Test background Tumor necrosis factor is a type of active cytokine with inflammatory mediating effects. It can be divided into two types, α and β, according to its cell source and molecular structure. TNF-α is produced by activated monocytes / macrophages and can be induced by hemorrhage, hypoxia, ischemia / reperfusion, lipopolysaccharide and complement system. It can lead to increased synthesis of nitric oxide (NO), activate the arachidonic acid pathway, induce the activation of cyclooxygenase and lipoxygenase, lead to the production of thromboxane A2 and prostaglandin E2; induce the production of selectins, PFA and intercellular adhesion molecule (ICAM), thereby mediating the migration of neutrophils to tissues; induce the production of a large number of cytokines such as IL-8, IL-6, IFN-y, IL-10; can activate multiple signal transduction pathways, kinases and transcription factors, activate a large number of cell genes, and produce a wide range of biological effects.

[0049] 2) Test methods 1. Cell seeding: Adjust the density of keratinocyte (HaCaT) suspension to 3×10 4 After that, the cells were inoculated into 96-well plates and cultured in a 37°C, 5% CO2 incubator for 24 h.

[0050] 2. Experimental grouping and sample addition: set up a negative control group, a positive control group and a sample test group.

[0051] 3. Inflammation induction: Centrifuge the P. acnes stock solution at 10,000 g for 10 min, remove the supernatant, wash with sterilized DPBS 2-3 times, centrifuge at 10,000 g for 10 min after each wash, and remove the supernatant. Add 100 μL / well of 0.5 OD P. acnes to the HaCaT cell well plates of the sample test group and the positive control group, and add an equal amount of culture medium to the negative control group.

[0052] 4. Sample addition: dilute the sample solution (i.e., the anti-inflammatory and soothing composition in the above embodiments and comparative examples) with culture medium to 10% and add it to the sample test group well plate. Add equal amounts of culture medium to the negative control group and the positive control group, and continue to culture in a 37°C, 5% CO2 incubator for 24 hours.

[0053] 5. ELISA test: The operation steps are as follows: A. Calculate the required enzyme label strips in advance, take out the kit 30 minutes before the experiment, and restore it to room temperature.

[0054] B. Serial dilution of the standard: dilute the standard with standard & sample diluent in the following ratios: 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 0 pg / mL.

[0055] C. Collect the cell culture supernatant into a sterile centrifuge tube and centrifuge (4°C, 1000×g, 20 min). Take the supernatant and dilute it 20 times as the test sample.

[0056] D. Add 100 μL of standard working solution and test sample to each reaction well. Set up 2 replicate wells for each group and incubate in a 37°C incubator for 90 min.

[0057] E. Discard the liquid, spin dry, add 100 μL of biotin-labeled TNF-α antibody working solution to each reaction well, and incubate in a 37°C incubator for 60 min.

[0058] F. Discard the liquid, spin dry, add 300 μL of washing solution to each reaction well, soak for 1-2 minutes and spin dry. Repeat 4 times.

[0059] G. Add 100 μL of HRP-labeled streptavidin working solution to each reaction well and incubate in a 37°C incubator for 30 min.

[0060] H. Add 300 μL of washing solution to each reaction well, spin dry the washing solution after 30 seconds, and repeat 4 times.

[0061] I. Add 90 μL of color developer to each reaction well and develop the color at 37°C in the dark for about 15 min.

[0062] J. Add 50 μL of stop solution to each reaction well and immediately measure the OD value at a wavelength of 450 nm using an ELISA reader.

[0063] K. Calculate the standard curve regression equation (R) based on the known concentration of the standard and the measured OD value. 2 >0.99), substitute the OD value of the sample well into the calculated concentration of the sample, and then multiply by the dilution factor to obtain the actual TNF-α concentration of the original sample.

[0064] 6. Statistical analysis: Use Excel software to perform statistical analysis of data and draw charts.

[0065] 3) Test results Calculation formula: T%= (T0-Tx) / T0×100%; Where T represents the inhibition rate of TNF-α in HaCaT cells; Tx represents the TNF-α content of the sample test group after the HaCaT cell test; T0 represents the TNF-α content in the positive control group after HaCaT cell test.

[0066] The data are sorted to obtain the following Table 2 and Figure 1 shown.

[0067] Table 2: TNF-α inhibition rate of HaCaT cells

[0068] from Figure 1 It can be seen from Table 2 that the composition provided in the example significantly inhibits the expression of TNF-α in HaCaT cells. By comparing the data between the comparative example and the example, it can be seen that the components of the composition of the present invention have a synergistic effect.

[0069] ②Inhibitory effect of the combination on IL-8 1) Test principle: Interleukin-8 (IL-8) is a potent chemotactic and proinflammatory cytokine produced in the skin by a variety of cells in response to inflammatory stimuli. The actions of IL-8 are mediated by binding to two types of specific high-affinity receptors that contain seven transmembrane domains typical of guanine nucleotide-binding protein (G protein)-coupled receptors. In the skin, a broad spectrum of cells including neutrophils, T lymphocytes, mast cells, dermal macrophages, endothelial cells, and keratinocytes possess binding sites for IL-8. Because the actions of IL-8 can be modulated at the receptor level, pharmacological manipulation of the IL-8 receptor may prove to be an important target for the treatment of skin diseases in which IL-8 levels are elevated.

[0070] 2) Testing process: 1. Cell seeding: Adjust the density of keratinocyte (HaCaT) suspension to 3×10 4 After that, the cells were inoculated into 96-well plates and cultured in a 37°C, 5% CO2 incubator for 24 h.

[0071] 2. Experimental grouping and sample addition: set up a negative control group, a positive control group and a sample test group.

[0072] 3. Inflammation induction: Centrifuge the P. acnes stock solution at 10,000 g for 10 min, remove the supernatant, wash with sterilized DPBS 2-3 times, centrifuge at 10,000 g for 10 min after each wash, and remove the supernatant. Add 100 μL / well of 0.5 OD P. acnes to the sample test group and the positive control group HaCaT cell well plate, and add an equal amount of culture medium to the negative control group.

[0073] 4. Sample addition: dilute the sample solution (i.e., the anti-inflammatory soothing composition in the above embodiments and comparative examples) with culture medium to the test concentration and add it to the sample test group well plate. Add equal amounts of culture medium to the negative control group and the positive control group, and continue to culture in a 37°C, 5% CO2 incubator for 24 hours.

[0074] 5. ELISA test: The operation steps are as follows: A. Calculate the required enzyme label strips in advance, take out the kit 30 minutes before the experiment, and restore it to room temperature.

[0075] B. Serial dilution of standard: Use standard & sample diluent to dilute the standard in the following steps: 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 0 pg / mL.

[0076] C. Collect the cell culture supernatant into a sterile centrifuge tube and centrifuge (4°C, 1000×g, 20 min). Take the supernatant and dilute it 20 times as the test sample.

[0077] D. Add 100 μL of standard working solution and test sample to each reaction well. Set up 2 replicate wells for each group and incubate in a 37°C incubator for 90 min.

[0078] E. Discard the liquid, spin dry, add 100 μL of biotin-labeled interleukin-8 antibody working solution to each reaction well, and incubate in a 37°C incubator for 60 min.

[0079] F. Discard the liquid, spin dry, add 300 μL of washing solution to each reaction well, soak for 1-2 minutes and spin dry, repeat 4 times.

[0080] G. Add 100 μL of HRP-labeled streptavidin working solution to each reaction well and incubate in a 37°C incubator for 30 min.

[0081] H. Add 300 μL of washing solution to each reaction well, spin dry the washing solution after 30 seconds, and repeat 4 times.

[0082] I. Add 90 μL of color developer to each reaction well and develop the color at 37°C in the dark for about 15 min.

[0083] J. Add 50 μL of stop solution to each reaction well and immediately measure the OD value at a wavelength of 450 nm using an ELISA reader.

[0084] K. Calculate the standard curve regression equation (R) based on the known concentration of the standard and the measured OD value. 2 >0.99), substitute the OD value of the sample well into the calculated concentration of the sample, and then multiply it by the dilution factor to get the actual IL-8 concentration of the original sample.

[0085] 6. Statistical analysis: Use Excel software to perform statistical analysis of data and draw charts.

[0086] 3) Test results: Calculation formula: C%= (C0-Cx) / C0×100%; Where C represents the inhibition rate of IL-8 in HaCaT cells; Cx represents the average IL-8 concentration in the sample test group after the HaCaT cell assay; C0 represents the average IL-8 concentration in the positive control group after HaCaT cell assay.

[0087] The data are sorted to obtain the following Table 3 and Figure 2 shown.

[0088] Table 3: IL-8 inhibition rate of HaCaT cells

[0089] from Figure 2 It can be seen from Table 3 that the composition provided in the example significantly inhibits the expression of IL-8 in HaCaT cells. By comparing the data between the comparative example and the example, it can be seen that the components of the composition of the present invention have a synergistic effect.

[0090] ③The effect of the combination on red acne marks 1) Test background: Example 4 and Comparative Example 3 were selected as test samples for human skin testing. Five adult female volunteers aged 18 to 40 with acne scars were selected. They used the test samples for 28 consecutive days under normal conditions, and the samples of Example 4 and Comparative Example 3 were randomly assigned to the left and right faces. The acne scars selected by the dermatologist were tested by the MINOLTA skin color difference test probe ChromameterTM CR400. The test time points were before using the sample, 3 days after using the sample, 7 days, 14 days, and 28 days after using the sample. The smaller the measured value, the less red the skin color.

[0091] 2) Test process: Two weeks before the test, the subjects stopped using cosmetics or topical drugs; the night before the test, the subjects did not apply any skin care products on their faces after washing their faces at home; on the morning of the test, the subjects did not apply any skin care products on their faces after washing their faces with clean water at home; the qualified subjects filled out the informed consent form; the included subjects waited for 30 minutes in an environment with a temperature of 21±1℃ and a relative humidity of 50±10%RH. The experimenter technician used the skin color difference test probe ChromameterTM CR400 to detect the skin color (red-green) value a* value, and used the facial image analyzer VISIA7 to collect images of the subjects. The subjects were revisited on the 3rd, 7th, 14th and 28th days. Excel software was used for data statistical analysis and drawing charts.

[0092] 3) Test results: Calculation formula: A%=(At-A0) / A0×100%; A: skin color (red-green) value a* value change rate; At: a* value of skin color after using the sample; A0: a* value of skin color before using the sample.

[0093] The test results are as follows Figure 3 , Figure 4 As shown. Figure 3 , Figure 4 It can be seen that both Example 4 and Comparative Example 3 are effective in inhibiting red acne marks. By comparing the data between Example 4 and Comparative Example 3, it can be seen that the components of the composition of the present invention have a synergistic effect.

[0094] It can be seen from the above test results that the anti-inflammatory and soothing compositions in Examples 1-6 have significant effects, which are significantly better than the compositions in Comparative Examples 1-6, and can be widely used in skin care products and cosmetics. When used, they can be added to skin care products and cosmetics in the form of aqueous solutions. Other components of skin care products and cosmetics can be existing conventional components, which are not limited in the present invention.

Claims

1. An anti-inflammatory soothing composition for repairing red acne marks, characterized in that: In parts by weight, it includes the following components: Kava extract 0.01-1 part; 0.05-1 part of Stephania tetrandra extract; Citrus fruit extract 0.01-2 parts; Dipotassium glycyrrhizinate 0.01-0.5 parts; Allantoin 0.01-0.5 parts; Hydrolyzed red algae extract 0.01-1 part.

2. The anti-inflammatory soothing composition according to claim 1, characterized in that: In parts by weight, it includes the following components: Kava extract 0.1-0.6 parts; 0.1-0.6 parts of Stephania tetrandra extract; Citrus fruit extract 0.1-1.5 parts; Dipotassium glycyrrhizinate 0.1-0.25 parts; Allantoin 0.05-0.25 parts; Hydrolyzed red algae extract 0.05-0.5 parts.

3. The anti-inflammatory soothing composition according to claim 2, characterized in that: The weight ratio of the citrus fruit extract to the total weight of the Kava Piper melongena extract and the Tetrandrakia tetrandra extract is not less than 0.

8.

4. The anti-inflammatory soothing composition according to any one of claims 1 to 3, characterized in that: The extraction method of the Piper methysticum extract comprises the following steps: crushing Piper methysticum to obtain Piper methysticum powder, adding the powder to a polyol solution, stirring and dispersing the powder evenly, performing high-pressure homogenization, performing ultrasonic treatment, performing extraction at room temperature, and obtaining the Piper methysticum extract after solid-liquid separation.

5. The anti-inflammatory soothing composition according to claim 4, characterized in that: The polyol solution is one or more of propylene glycol, butylene glycol, hexylene glycol and pentylene glycol, the mass content of the polyol in the polyol solution is 20-80%, and the mass content of gum arabic not exceeding 10% is further added to the polyol solution, and the mass concentration of the kava pepper in the polyol solution is 5-10%.

6. The anti-inflammatory soothing composition according to claim 4, characterized in that: The homogenization time during high-pressure homogenization treatment is 3-5 minutes, the homogenization pressure is 200-400 Bar, and the homogenization is performed multiple times; the ultrasonic time during ultrasonic treatment is 30-60 minutes, and the ultrasonic temperature is 30-40°C; the single extraction time during extraction treatment is 20-24 hours, and the extraction is performed multiple times.

7. Use of the anti-inflammatory soothing composition according to any one of claims 1 to 6 in skin care products.

8. A skin care product, characterized in that: Its components include the anti-inflammatory and soothing composition described in any one of claims 1 to 6.

9. Use of the anti-inflammatory soothing composition according to any one of claims 1 to 6 in cosmetics.

10. A cosmetic, characterized in that: Its components include the anti-inflammatory and soothing composition described in any one of claims 1 to 6.

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