Chinese herbal medicine exosome complex, preparation method and application

Abstract

The present invention relates to a Chinese herbal medicine exosome complex, a preparation method and an application, and relates to the technical field of plant exosomes. The Chinese herbal medicine exosome complex comprises Chinese herbal medicine exosomes, wherein the Chinese herbal medicine exosomes comprise rose exosomes, ginseng exosomes and Centella asiatica exosomes, and the mass ratio of the rose exosomes, ginseng exosomes and Centella asiatica exosomes is (1.5-3):(1-3):1. The Chinese herbal medicine exosome complex provided by the present invention innovatively compounds rose exosomes, Centella asiatica exosomes and ginseng exosomes, wherein the herbal formula can not only obtain a safe composite formula preparation, but also the three can play a role of mutual synergy and mutual promotion, and through the compounding of the three in a specific ratio, it achieves unexpected effects in anti-aging effect, whitening effect and anti-inflammatory effect.

Description

Technical Field

[0001] The present invention relates to the technical field of plant exosomes, and specifically to a Chinese herbal medicine exosome complex, a preparation method and an application thereof. Background Art

[0002] At present, most beauty products and cosmetics on the market use chemical synthetic ingredients, which may cause irritation or other side effects to the skin if used for a long time. Chinese herbal medicine is a valuable asset unique to China, with a long history of research. Compared with chemical synthetic ingredients, Chinese herbal medicine is often safer and can be used not only as a drug to treat diseases, but also as a food for health care. However, due to the complex ingredients, the quality control of Chinese herbal medicine is relatively difficult.

[0003] Plant exosomes are nano-scale vesicles secreted by plant cells. They contain a variety of bioactive substances and have the functions of promoting skin cell regeneration, anti-oxidation, anti-inflammation and repairing skin barriers. The quality of exosomes can be controlled by various technologies such as transmission electron microscopy. Therefore, the use of Chinese herbal plant exosomes can not only remove excess impurities and enrich active substances, but also standardize and prepare them on a large scale. How to explore the characteristics of Chinese herbal exosomes and prepare safe and effective beauty, cosmetics or health products is a question worth exploring. Summary of the invention

[0004] The purpose of the present invention is to provide a Chinese herbal exosome complex, a preparation method and an application thereof, aiming to fully explore the characteristics of Chinese herbal exosomes to prepare safe and effective beauty, cosmetic products or health products.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A first aspect of the present invention provides a Chinese herbal exosome complex, comprising Chinese herbal exosomes, wherein the Chinese herbal exosomes include rose exosomes, ginseng exosomes and Centella asiatica exosomes.

[0007] In a preferred embodiment, in the Chinese herbal exosomes, the mass ratio of rose exosomes, ginseng exosomes and Centella asiatica exosomes is (1.5-3):(1-3):1.

[0008] In a preferred embodiment, the Chinese herbal exosome complex further comprises glabridin, and in terms of weight, the Chinese herbal exosomes are 5-15 parts, and the glabridin is 1.5-5 parts.

[0009] In a preferred embodiment, the Chinese herbal exosome complex further comprises hyaluronic acid. In terms of weight, the Chinese herbal exosomes are 5-15 parts, and the hyaluronic acid is 0.03-0.08 parts.

[0010] In a preferred embodiment, the Chinese herbal exosome complex further comprises a small molecule peptide. In terms of weight, the Chinese herbal exosomes account for 5-15 parts, and the small molecule peptide accounts for 0.07-0.3 parts.

[0011] In a preferred embodiment, the small molecule peptide comprises: a combination of one or more of oligopeptide-1, pentapeptide-18, and acetyl hexapeptide-8.

[0012] In a preferred embodiment, the Chinese herbal exosome complex further comprises 7-20 parts of a buffer and preservative system and water, and the Chinese herbal exosomes are 5-15 parts.

[0013] In a preferred embodiment, the buffer and preservative system comprises: a combination of one or more of an amino acid mixture, 1,2-pentanediol, and 1,2-hexanediol.

[0014] The second aspect of the present invention provides a method for preparing the Chinese herbal medicine exosome complex, comprising the following steps:

[0015] S1, heating the components other than the Chinese herbal exosomes and stirring them evenly until they are completely dissolved;

[0016] S2, after cooling, add the Chinese herbal exosomes and stir evenly.

[0017] The third aspect of the present invention provides the use of the Chinese herbal exosome complex in beauty, cosmetic products and / or health products.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The Chinese herbal exosome complex provided by the present invention innovatively compounds rose exosomes, Centella asiatica exosomes and ginseng exosomes, wherein the herbal formula can not only obtain a safe composite formula preparation, but also the three can play a synergistic and mutually reinforcing role. Through the compounding of the three in a specific ratio, it can achieve unexpected effects in anti-aging, whitening and anti-inflammatory effects. The specific interaction mechanism will be described in detail below. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A comparative diagram of the indicators of rose exosomes, ginseng exosomes and Centella asiatica exosomes promoting the production of COL1A1;

[0021] Figure 2 A comparison chart showing the indicators of rose exosomes, ginseng exosomes and Centella asiatica exosomes inhibiting the expression of MMP1;

[0022] Figure 3A comparative diagram of the indicators of rose exosomes, ginseng exosomes and Centella asiatica exosomes in inhibiting melanin production;

[0023] Figure 4 This is a graph of the particle concentration and size of rose exosomes;

[0024] Figure 5 This is the morphology of rose exosomes;

[0025] Figure 6 Figure 2 shows the particle concentration and particle size of Centella asiatica exosomes;

[0026] Figure 7 This is the morphology of Centella asiatica exosomes;

[0027] Figure 8 This is a graph of particle concentration and particle size of ginseng exosomes;

[0028] Fig. 9 This is the morphological diagram of ginseng exosomes;

[0029] Fig.10 A comparative diagram of the indicators of promoting the production of COL1A1 by the formulas provided in Example 1 and Comparative Examples 1-3;

[0030] Fig.11 A comparative diagram of the indicators of inhibiting MMP1 expression of the formulas provided in Example 1 and Comparative Examples 1-3;

[0031] Fig.12 A comparative diagram of the indexes of inhibiting melanin production of the formulas provided in Example 1 and Comparative Examples 1-3;

[0032] Fig.13 A comparative diagram of the indicators of inhibiting the expression of inflammatory factors by the formulas provided in Example 1 and Comparative Examples 1-3;

[0033] Fig.14 A comparative diagram of the indicators of promoting the production of COL1A1 by the formulas provided in each embodiment and comparative examples 4-7;

[0034] Fig.15 A comparative diagram of the indexes of inhibiting melanin production of the formulas provided in each embodiment and comparative examples 4-7;

[0035] Fig.16 It is a comparative diagram of the indicators of inhibiting the expression of inflammatory factors by the formulas provided in each embodiment and comparative examples 4-7. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] A first aspect of the present invention provides a Chinese herbal exosome complex, comprising Chinese herbal exosomes, wherein the Chinese herbal exosomes further comprise rose exosomes, ginseng exosomes and Centella asiatica exosomes.

[0038] The applicant will explain the effects of the combination of rose exosomes, ginseng exosomes and Centella asiatica exosomes from three aspects: anti-aging effect, whitening effect and anti-inflammatory effect.

[0039] 1. Anti-aging effect

[0040] Collagen (COL1A) accounts for 70% of the total composition of human dermis. Its reticular structure provides protection and elasticity for the skin. A large amount of water, extracellular matrix and functional cells are distributed between the fibers. It is an important biochemical reaction site for the skin and provides moisture and nutrition for the epidermis. Matrix metalloproteinase (MMP1) can lead to excessive degradation of collagen and elastin in the dermis that support the skin structure, resulting in aging symptoms such as wrinkles and inelasticity. Therefore, promoting the production of COL1A and inhibiting the expression of MMP1 are important indicators for verifying anti-aging efficacy.

[0041] When 3E10 particles / ml of rose exosomes, ginseng exosomes and Centella asiatica exosomes were simultaneously applied to fibroblasts, ginseng exosomes and rose exosomes were able to significantly promote the production of type I collagen (COL1A1) and inhibit the expression of MMP1, and ginseng exosomes had the best effect. The specific results are as follows: Figure 1 and Figure 2 As shown, the NC group was the negative control group.

[0042] At the same time, rose exosomes, ginseng exosomes and Centella asiatica exosomes each have unique biological activities, and they show certain interactions in skin repair and anti-aging.

[0043] Specifically, Centella asiatica exosomes can strengthen the epidermal skin barrier, improve the epidermis's defense against the external environment, protect the skin from external stimulation and damage, and provide a more stable skin environment for subsequent repair; on the one hand, ginseng exosomes can increase the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH2Px) in the body, induce the expression of antioxidant enzyme genes, and reduce the damage of lipid peroxidation products (such as malondialdehyde MDA) to epidermal cells. On the other hand, ginseng exosomes can affect the activity of cell cycle regulators and telomerase, and prolong cell life; rose exosomes have a low half-inhibitory concentration (IC50) for ·OH and DPPH free radicals, proving that rose exosomes have strong antioxidant activity. Therefore, Centella asiatica exosomes and rose exosomes help ginseng exosomes to increase the activity of antioxidant enzymes in the epidermis and basal layer, synergistically inhibit cell apoptosis caused by oxidative stress, and jointly promote the proliferation of basal fibroblasts and COL1A production, restoring skin elasticity and firmness.

[0044] 2. Whitening effect

[0045] The most important determinant of skin color comes from melanin in melanosomes. The activity of basal melanocytes (the quantity and quality of melanin produced) determines the color of the skin to the greatest extent.

[0046] When 3E10 particles / ml of rose exosomes, ginseng exosomes and Centella asiatica exosomes were simultaneously applied to B16 cells induced by 0.2μmol / L α-melanocyte stimulating hormone (α-MSH) to produce melanin, rose exosomes and ginseng exosomes were able to significantly inhibit the production of melanin, and rose exosomes had the best effect. The results are as follows: Figure 3 As shown, PC was the positive control group.

[0047] At the same time, rose exosomes, ginseng exosomes and Centella asiatica exosomes showed certain interactions in inhibiting melanin production and promoting melanin decomposition.

[0048] Specifically, Centella asiatica exosomes inhibit the expression of melanin synthase genes by reducing the affinity of transcription factor MITF to DNA, thereby reducing pigmentation; ginseng exosomes reduce melanin content and inhibit tyrosinase activity by regulating the signaling molecules Akt and extracellular signal-regulated kinase for melanin production; rose exosomes can inhibit the expression of NGF and FGF2 genes upstream of the melanin production pathway, reduce the proliferation of melanocytes, and thus reduce pigmentation. NGF activates phosphatidylinositol-3 kinase (PI3K) by binding to its high-affinity receptor TrkA, and PI3K further activates Akt, which inhibits apoptosis and promotes cell survival by phosphorylating a series of downstream target proteins, such as FOXO3a. Therefore, rose exosomes can inhibit Akt by inhibiting the expression of the NGF gene, thereby promoting the whitening effect of ginseng exosomes.

[0049] Moreover, the nutrients in rose exosomes, ginseng exosomes and Centella asiatica exosomes can synergistically promote skin metabolism, accelerate the shedding of aging cells and the generation of new cells, and make the skin look smoother and more delicate.

[0050] 3. Anti-inflammatory effect

[0051] In the event of tissue damage, bacterial infection or sterile inflammation, macrophages (Raw264.7) secrete a series of inflammatory cytokines, including interleukin-6 (IL-6). Excessive production of inflammatory factors can cause harmful effects on tissues and organisms, and is associated with the pathogenesis of various inflammation-related diseases, including skin inflammation, acne and other problems.

[0052] 1ug / ml LPS was used to induce Raw264.7 cells to release the inflammatory factor IL6, and then 3E10 particle / ml of rose exosomes, ginseng exosomes and Centella asiatica exosomes were simultaneously applied to the inflammatory state of Raw264.7 cells. The results showed that rose exosomes and Centella asiatica exosomes could significantly inhibit the expression of inflammatory factor IL6, among which Centella asiatica exosomes had the best effect.

[0053] Rose exosomes, ginseng exosomes and Centella asiatica exosomes contain a large number of active ingredients with anti-inflammatory effects. Centella asiatica exosomes can reduce the inflammatory response signaling pathway by inhibiting the NF-κB and MAPK signaling pathways, and downregulate the expression of inflammatory factors such as TNF-α, IL-1β, and IL-6, thereby exerting an anti-inflammatory effect. Rose exosomes can inhibit apoptosis and inflammation by regulating the PI3K / AKT signaling pathway and the endoplasmic reticulum stress pathway. The MAPK and PI3K / AKT signaling pathways can work synergistically through different mechanisms.

[0054] On the one hand, inhibiting the MAPK and PI3K / AKT signaling pathways can directly reduce the production of inflammatory factors. On the other hand, both the MAPK and PI3K / AKT signaling pathways can regulate the NF-κB signaling pathway and inhibit the activation of NF-κB, thereby reducing the production of inflammatory factors. The PI3K / AKT signaling pathway prevents the activation of NF-κB by increasing the phosphorylation of IκBα, while the MAPK signaling pathway regulates the transcriptional activation of the NF-κB pathway. Ginseng exosomes reduce the production of inflammatory mediators by inhibiting the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), while providing nutrients to promote the repair and proliferation of damaged cells.

[0055] Preferably, in the present invention, the mass ratio of rose exosomes, ginseng exosomes and Centella asiatica exosomes is (1.5-3): (1-3): 1. Within this ratio range, good effects can be achieved in anti-aging, whitening and anti-inflammatory aspects. Due to the interaction between the three, if rose exosomes and ginseng exosomes are added in excess, the effect will not increase with the amount, while excessive addition of Centella asiatica exosomes will not significantly increase the anti-inflammatory and whitening effects, and may cause side effects such as local irritation and itching.

[0056] Preferably, in the present invention, the Chinese herbal exosome complex also includes glabridin, and the Chinese herbal exosomes are 5-15 parts by weight, and the glabridin is 1.5-5 parts. Glabridin is an isoflavone compound, mainly derived from the extraction of the root of Glycyrrhiza glabra L., and has whitening, anti-inflammatory, antioxidant and other activities. Chinese herbal exosomes can improve the bioavailability of glabridin, enhance its whitening and anti-inflammatory effects, and inhibit the synthesis of melanin by targeting melanocytes. Rose exosomes and ginseng exosomes have similar effects, and Chinese herbal exosomes, as a natural nanocarrier, can encapsulate glabridin, improve its penetration and targeting in the skin, thereby enhancing the whitening and anti-inflammatory effects of glabridin. In addition, Chinese herbal exosomes have the characteristic of being easy to penetrate cell membranes, so they have natural advantages as drug delivery carriers, which can improve the stability of glabridin and protect it from rapid decomposition during transportation in the body, thereby prolonging the action time of glabridin in the body. Glabridin has a high safety factor, and the dose of glabridin added to the composite of the present invention has no toxic side effects, and an overdose may cause allergic reactions.

[0057] Preferably, in the present invention, the Chinese herbal exosome complex also includes hyaluronic acid, and the Chinese herbal exosome is 5-15 parts by weight, and the hyaluronic acid is 0.03-0.08 parts. Hyaluronic acid has a moisturizing component that can absorb and lock a large amount of water, providing deep moisturizing for the skin. As a carrier, Chinese herbal exosomes can carry hyaluronic acid molecules to help them penetrate into the deep layers of the skin more effectively, thereby enhancing the moisturizing effect. Hyaluronic acid also has anti-inflammatory properties and can help reduce skin inflammation, while Chinese herbal exosomes also have anti-inflammatory effects. They can work synergistically to reduce skin redness and inflammatory reactions, especially after water light needle treatment, reducing possible side effects. Hyaluronic acid helps repair skin damage, while Chinese herbal exosomes can carry growth factors and other bioactive molecules to promote the proliferation and migration of skin cells and accelerate the wound healing process. In water light needles, the combined use of hyaluronic acid and Chinese herbal exosomes can improve the therapeutic effect, provide more lasting moisturizing and anti-aging effects, and reduce the frequency and dosage of injections. It should be noted that excessive injection of hyaluronic acid may make it difficult for local skin to absorb, resulting in "pancake-like" cheeks, looser skin, swelling, redness, bruising and other symptoms, and may also cause allergic reactions.

[0058] Preferably, in the present invention, the Chinese herbal medicine exosome complex also includes a small molecule peptide, and the Chinese herbal medicine exosome is 5-15 parts by weight, and the small molecule peptide is 0.07-0.3 parts. The interaction between small molecule peptides and plant exosomes in water light needles is mainly manifested in improving transdermal effect, enhancing stability and biological activity, targeted delivery, anti-inflammatory and immunomodulation, promoting collagen production, and enhancing drug efficacy. Small molecule peptides can quickly penetrate the skin in water light needles due to their small molecules and easy absorption. Chinese herbal medicine exosomes, as a nanocarrier, can carry small molecule peptides, enhance their transdermal effect by membrane fusion, and improve the bioavailability of small molecule peptides. At the same time, Chinese herbal medicine exosomes can protect small molecule peptides from degradation and inactivation in the extracellular environment, and maintain their structural stability and biological activity. In addition, rose exosomes and ginseng exosomes have anti-inflammatory and collagen degradation inhibition effects, and some small molecule peptides also have similar effects. The combination of the two can play a synergistic role. Small molecule peptides have a higher safety factor, but large overdoses may cause allergic reactions and endocrine disorders.

[0059] Preferably, the small molecule peptide includes a combination of one or more of oligopeptide-1, pentapeptide-18, and acetyl hexapeptide-8.

[0060] Preferably, in the present invention, the Chinese herbal exosome complex further comprises 7-20 parts of a buffer and preservative system and 70-80 parts of water, wherein the buffer and preservative system comprises a combination of one or more of an amino acid mixture, 1,2-pentanediol, and 1,2-hexanediol. The overall stability of the complex is enhanced by the buffer and preservative system.

[0061] The second aspect of the present invention provides a method for preparing a Chinese herbal medicine exosome complex, comprising the following steps:

[0062] S1, heating other components except Chinese herbal exosomes, and stirring evenly until all dissolved (when other components are included);

[0063] Specifically, the components other than the Chinese herbal exosomes and the amino acid mixture are heated to 75-90°C, stirred evenly until all dissolved, kept warm for 30 minutes, and then cooled to 60-65°C and added to the amino acid mixture.

[0064] S2, when the temperature is lowered to 45° C., the Chinese herbal exosomes are added and stirred evenly. The appropriate temperature can prevent the inactivation of proteins or small molecules carried by the Chinese herbal exosomes.

[0065] The third aspect of the present invention provides the use of the aforementioned Chinese herbal exosome complex in beauty, cosmetic products and / or health products, such as lyophilized powder, hyaluronic acid injection, cosmetics or health products.

[0066] The present invention is further described in detail below with reference to the embodiments. In the following embodiments and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified.

[0067] In the following examples and comparative examples, Chinese herbal exosomes were extracted using the following method:

[0068] 1. Rose exosomes, using dark red roses rich in active ingredients:

[0069] Remove the calyx and keep the petals, sterilize with ultrasound for 30 minutes, soak in Watsons pure water for 30 minutes, discard the pure water and let the raw materials stand, a little pure water may remain;

[0070] Juicing with a juicer: Raw materials: physiological saline = 300g: 1000ml. After juicing, pour 250ml of physiological saline into the juicer to wash twice (a total of 500ml), and finally get about 1800ml of raw material liquid;

[0071] Coarse filtration: Pour the raw material liquid into the filter bag and squeeze out 1800ml of coarse filtrate, and discard the filter residue;

[0072] Centrifugation: After aliquoting, centrifuge at 12000g for 30 min, take the supernatant and discard the precipitate;

[0073] Filtration: The supernatant was filtered through a 0.45 μm PVDF cup filter to obtain a filtrate, the stock solution was diluted to 5E9-3E10, and the exosomes were purified using the existing Exodus equipment. The concentration and particle size of the exosome particles were detected by NTA. The results are as follows: Figure 4 As shown, TEM technology was used to identify the morphology of exosomes. The results are as follows Figure 5 shown.

[0074] 2. Centella asiatica exosomes:

[0075] Remove roots and impurities, leaving fresh stems and leaves, wash with Watsons pure water until the water is clear, ultrasonically sterilize for 30 minutes, and soak in Watsons pure water for 30 minutes;

[0076] Juicing with a juicer: Raw materials: physiological saline = 300g: 1000ml. After juicing, pour 250ml of physiological saline into the juicer to wash twice (a total of 500ml), and finally get about 1500ml of raw material liquid;

[0077] Coarse filtration: Pour the raw material liquid into the filter bag and squeeze out 1500ml of coarse filtrate, and discard the filter residue;

[0078] Centrifugation: After aliquoting, centrifuge at 12000g for 30 min, take the supernatant and discard the precipitate;

[0079] Filtration: The supernatant was filtered through a 0.45 μm PVDF cup filter to obtain a filtrate, the stock solution was diluted to 5E9-3E10, and the exosomes were purified using the existing Exodus equipment. The concentration and particle size of the exosome particles were detected by NTA. The results are as follows: Figure 6 As shown, TEM technology was used to identify the morphology of exosomes. The results are as follows Figure 7 shown.

[0080] 3. Ginseng exosomes, using Northeast ginseng rich in active ingredients:

[0081] The ginseng roots and main body were cut and separated, washed with Watsons pure water until the water was clear, ultrasonically sterilized for 30 minutes, and soaked in Watsons pure water for 30 minutes;

[0082] Juicing with a juicer: Raw materials: physiological saline = 300g: 1000ml. After juicing, pour 250ml of physiological saline into the juicer to wash twice (a total of 500ml), and finally get about 1500ml of raw material liquid;

[0083] Coarse filtration: Pour the raw material liquid into the filter bag and squeeze out 1500ml of coarse filtrate, and discard the filter residue;

[0084] Centrifugation: After aliquoting, centrifuge at 12000g for 30 min, take the supernatant and discard the precipitate;

[0085] Filtration: The supernatant was filtered through a 0.45 μm PVDF cup filter to obtain a filtrate, the stock solution was diluted to 5E9-3E10, and the exosomes were purified using the existing Exodus equipment. The concentration and particle size of the exosome particles were detected by NTA. The results are as follows: Figure 8 As shown, TEM technology was used to identify the morphology of exosomes. The results are as follows Fig. 9 shown.

[0086] The application states and formulations of the Chinese herbal exosome complexes provided in Examples 1 to 3 and Comparative Examples 1 to 7 are shown in Table 1:

[0087] Table 1

[0088]

[0089] 1. Verification of anti-aging effects

[0090] Based on the compound formula of Example 1 and the single formula of Comparative Examples 1-3, the anti-aging effects were verified on the cell model. Fig.10 It is an indicator of COL1A (collagen) production. Fig.11 It is an indicator for inhibiting the expression of MMP1 (matrix metalloproteinase). Fig.10 and Fig.11 It can be seen from the comparison diagram that the combined formula of rose exosomes, ginseng exosomes and Centella asiatica exosomes provided in Example 1 (Product 1) is significantly better than the single formula provided in Comparative Examples 1-3 in terms of COL1A production index, and is significantly better than Comparative Examples 1-3 in terms of MMP1 expression inhibition index.

[0091] 2. Verification of whitening efficacy

[0092] Based on the compound formula of Example 1 and the single formula of Comparative Examples 1-3, the effect of inhibiting melanin production on the cell model was verified. The verification results are as follows: Fig.12 As shown, PC is the positive control group. By comparing the diagrams, it can be seen that the compound formula (Product 1) of rose exosomes, ginseng exosomes and Centella asiatica exosomes provided in Example 1 is significantly better than the single formula provided in Comparative Examples 1-3 in inhibiting melanin production indicators.

[0093] 3. Verification of anti-inflammatory effects

[0094] Based on the compound formula of Example 1 and the single formula of Comparative Examples 1-3, the effect of inhibiting the expression of inflammatory factors on the cell model was verified. The verification results are as follows: Fig.13As shown, it can be seen from the comparative diagram that the compound formula (Product 1) of rose exosomes, ginseng exosomes and Centella asiatica exosomes provided in Example 1 is significantly better than the single formula provided in Comparative Examples 1-3 in inhibiting the expression of inflammatory factors.

[0095] 4. Component and ratio verification

[0096] Based on the compound formulas provided in Examples 1-3 and Comparative Examples 4-7, their anti-aging efficacy, melanin inhibition effect and inflammatory factor inhibition effect on cell models were verified respectively:

[0097] Anti-aging efficacy verification results such as Fig.14 As shown, it can be seen from the test results that the compound formulas provided in Comparative Examples 4 to Comparative Examples 6 have similar effects on promoting the production of type I collagen (COL1A1) as those of the embodiments. In Comparative Example 4, excessive addition of Centella asiatica exosomes has a relatively lower promoting effect, while excessive addition of ginseng exosomes or rose exosomes does not have an adverse effect on the anti-aging effect. However, outside the mass ratio set by the present invention, the corresponding effect will not increase with the increase of ginseng exosomes or rose exosomes.

[0098] The results of melanin inhibition effect verification are as follows Fig.15 As shown, it can be seen from the test results that the compound formulas provided by Comparative Examples 4 to Comparative Examples 6 have an inhibitory effect on melanin that is similar to that of the embodiments, which indicates that excessive addition of the three types of plant exosomes does not have an adverse effect on the melanin inhibitory effect, but outside the mass ratio set by the present invention, the corresponding effect will not increase with the increase of each plant exosome.

[0099] The results of the inflammatory factor inhibition effect verification are as follows Fig.16 As shown, it can be seen from the test results that the compound formulas provided by Comparative Examples 4 to Comparative Examples 6 have an inhibitory effect on inflammatory factors that is similar to or slightly worse than that of the embodiments, which indicates that excessive addition of the three types of plant exosomes does not have an adverse effect on the inhibitory effect of inflammatory factors, but outside the mass ratio set by the present invention, the corresponding effect will not increase with the increase of each plant exosome.

[0100] In Comparative Example 7, ginseng exosomes were replaced with ginger exosomes, and the anti-aging, whitening and anti-inflammatory effects were not as good as those in Example 1. It can be seen that the active ingredients between rose exosomes, ginseng exosomes and Centella asiatica exosomes can synergize and promote each other, so that they can achieve good effects in anti-aging, whitening and anti-inflammatory.

[0101] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A Chinese herbal medicine exosome complex, characterized in that: It includes Chinese herbal exosomes, and the Chinese herbal exosomes include rose exosomes, ginseng exosomes and Centella asiatica exosomes.

2. The Chinese herbal medicine exosome complex according to claim 1, characterized in that: Among the Chinese herbal exosomes, the mass ratio of rose exosomes, ginseng exosomes and Centella asiatica exosomes is (1.5-3):(1-3):

1.

3. The Chinese herbal medicine exosome complex according to claim 1, characterized in that: The Chinese herbal exosome complex also includes glabridin. In terms of weight, the Chinese herbal exosomes account for 5-15 parts, and the glabridin accounts for 1.5-5 parts.

4. The Chinese herbal medicine exosome complex according to claim 1, characterized in that: The Chinese herbal exosome complex also includes hyaluronic acid. In terms of weight, the Chinese herbal exosomes are 5-15 parts, and the hyaluronic acid is 0.03-0.08 parts.

5. The Chinese herbal medicine exosome complex according to claim 1, characterized in that: The Chinese herbal exosome complex also includes a small molecule peptide. In terms of weight, the Chinese herbal exosomes account for 5-15 parts, and the small molecule peptide accounts for 0.07-0.3 parts.

6. The Chinese herbal medicine exosome complex according to claim 5, characterized in that: The small molecule peptides include: a combination of one or more of oligopeptide-1, pentapeptide-18, and acetyl hexapeptide-8.

7. The Chinese herbal medicine exosome complex according to any one of claims 1 to 6, characterized in that: The Chinese herbal exosome complex further comprises 7-20 parts of a buffer and preservative system and water, and the Chinese herbal exosomes are 5-15 parts.

8. The Chinese herbal medicine exosome complex according to claim 7, characterized in that: The buffer and antiseptic system comprises: a combination of one or more of an amino acid mixture, 1,2-pentanediol, and 1,2-hexanediol.

9. The method for preparing the Chinese herbal medicine exosome complex according to any one of claims 3 to 8, characterized in that: The following steps are involved: S1, heating the other components except the Chinese herbal exosomes and stirring them evenly until they are completely dissolved; S2, after cooling, add the Chinese herbal exosomes and stir evenly.

10. Use of the Chinese herbal exosome complex according to any one of claims 1 to 8 in beauty, cosmetic products and / or health products.

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