A scalp care composition and application thereof

Abstract

The present invention belongs to the technical field of daily chemicals, and specifically relates to a scalp care composition and its application. The scalp care composition of the present invention includes the following components by mass percentage: 0.5-6.0% sodium hyaluronate, 0.2-4.0% of Dendrobium officinale hydrolysate, 1.0-3.0% pentanediol, 1.0-3.0% hexanediol and a galactose yeast-like bacteria fermentation product filtrate supplemented to a total mass percentage of 100%. The scalp care composition provided by the present invention has a reasonable proportion of each component and a synergistic effect, can effectively care for the scalp, has anti-inflammatory, moisturizing, and soothing effects, solves the problems of severe scalp oiliness, itchy scalp, and dandruff, and has the beneficial effects of nourishing the scalp, protecting the scalp barrier, and improving the scalp microecology.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemicals, and particularly relates to a scalp care composition and application thereof. Background Art

[0002] With the rapid development of production and economy, consumers pay more and more attention to health, and the high-level demand for hair products is gradually increasing. The thickness of the scalp is very thin, which is thinner than other parts of the body. It is the second thinnest skin in the whole body, second only to the skin around the eyes. The metabolism rate of the scalp is fast, and the keratinization and renewal rate of the scalp epidermis is relatively fast, about twice that of other skin. Hundreds of thousands of keratin cells are shed every day. Therefore, compared with other parts of the body, the scalp is more likely to accumulate old and dead skin cells, which can easily cause scalp itching, inflammation, and damage to the scalp barrier function.

[0003] At present, the scalp care products on the market are mainly wash-off scalp care products. While meeting the daily washing and care requirements, the hair is better conditioned by using mild surfactants, special oils, natural active ingredients and other ingredients, and the scalp is also provided with a certain care effect. However, wash-off scalp care products will remove the protective sebum on the scalp while cleaning the scalp, thereby damaging the barrier function of the scalp. Although oil conditioners are usually added to shampoo to reduce the dryness and tightness of the scalp, the oil conditioners will be washed away when the shampoo is rinsed, and the residence time on the scalp is short, the effect is not good, and the dryness and tightness of the scalp cannot be alleviated. Dry dandruff is prone to occur after long-term use. At present, products for scalp care have appeared on the market. CN111437208A discloses a scalp hair cleaning composition, a scalp care product, and a scalp care method, but the invention also suppresses skin inflammation by removing the sebum on the head, and cannot avoid the problem of long-term use destroying the original protective oil of the scalp. CN115501124A discloses a scalp care composition and a preparation method thereof and a scalp care agent, which soften and exfoliate old dead skin cells to solve dry scalp, dandruff and itchy scalp. However, frequent exfoliation of dead skin cells can also cause dry skin and cannot effectively maintain the scalp barrier.

[0004] Therefore, it is necessary to develop a scalp care product that can effectively protect and repair the scalp barrier, relieve the problems of oily scalp, itching, and dandruff, and have a good scalp care effect. Summary of the invention

[0005] To solve the above problems, the present invention provides a scalp care composition and its application, which can effectively protect and repair the scalp barrier, nourish the scalp, improve the scalp microecology, alleviate the problems of oily scalp, itching and dandruff, and have a good scalp care effect.

[0006] The first aspect of the present invention provides a scalp care composition, which comprises the following ingredients, by mass percentage: 0.5-6.0% sodium hyaluronate, 0.2-4.0% enzymatic hydrolysate of Dendrobium officinale, 1.0-3.0% pentylene glycol, 1.0-3.0% hexylene glycol and a filtrate of a galactosidase-like bacteria fermentation product supplemented to a total mass percentage of 100%.

[0007] The enzymatic hydrolysate of Dendrobium candidum contains polysaccharides, alkaloids and bibenzyls, amino acids and trace elements, and has certain pharmacological effects such as regulating immunity, lowering blood sugar, anti-oxidation, and anti-radiation. The filtrate of the fermentation product of galactosaccharomyces-like bacteria is rich in active ingredients such as free amino acids, minerals, organic acids, inorganic acids, oligosaccharides, pyruvic acid, and α-glucan oligosaccharides. The present invention reasonably proportions the two, better exerts the synergistic effect of the enzymatic hydrolysate of Dendrobium candidum and the filtrate of the fermentation product of galactosaccharomyces-like bacteria, can better regulate the scalp microecology, and enhance the scalp barrier.

[0008] Hyaluronic acid is widely present in the human body and is an important component of the skin, vitreous body, synovial fluid and cartilage tissue. It has unique physical and chemical properties and biological functions, such as good viscoelasticity, moisturizing, lubrication and anti-inflammatory activity.

[0009] Preferably, the molecular weight of the sodium hyaluronate is 600-1000Da. The present invention uses sodium hyaluronate with a very low molecular weight, which can penetrate into the scalp dermis and interact with cell surface receptors to regulate the synthesis of collagen and reduce the generation of inflammatory mediators.

[0010] Preferably, the pentanediol is 1,2-pentanediol.

[0011] Preferably, the hexanediol is 1,2-hexanediol.

[0012] The present invention adopts 1,2-pentanediol and 1,2-hexanediol as a highly effective moisturizing agent, penetration enhancer and antibacterial agent. 1,2-pentanediol and 1,2-hexanediol have excellent hygroscopicity and water retention, can reduce scalp water loss, improve the dry state of scalp, and reduce dandruff generation. The combined use of 1,2-pentanediol and 1,2-hexanediol can also promote the transdermal absorption of Dendrobium officinale hydrolysate and sodium hyaluronate, promote ultra-low molecular weight Dendrobium officinale polysaccharide and hyaluronic acid oligosaccharide to enter the dermis layer of the scalp, improve the interaction between these active substances and dermal cell surface receptors, and enhance the repair effect of the composition. 1,2-pentanediol and 1,2-hexanediol have good inhibitory effects on Gram-positive bacteria, Gram-negative bacteria, yeast and mold, can improve the stability of the scalp care composition and prevent it from deteriorating.

[0013] Preferably, the preparation method of the Dendrobium officinale hydrolysate is as follows:

[0014] S1, puffing, grinding, and extracting the stems of Dendrobium officinale to obtain a water extract of Dendrobium officinale;

[0015] S2, adjusting the pH of the Dendrobium officinale water extract to 5-6, adding a complex enzyme, performing enzymolysis, and filtering, and the obtained filtrate is the Dendrobium officinale enzymolysate.

[0016] Preferably, the puffing conditions in step S1 are as follows: pressure of 0.45-1.0Mpa, temperature of 50-80°C, and duration of 90-150min. The puffing method adopted in the present invention can destroy the cell wall of the stem of Dendrobium officinale, form loose pores inside the stem of Dendrobium officinale, and then degrade and change the structure of macromolecular substances such as polysaccharides, phenolic compounds and proteins constituting its cell wall, thereby facilitating the subsequent full contact of the stem of Dendrobium officinale with the solvent, and facilitating the full leaching of Dendrobium officinale polysaccharides, Dendrobium officinale alkaloids, sterols and other active substances.

[0017] Preferably, in step S1, the Dendrobium officinale stems are expanded and ground into powder to obtain the Dendrobium officinale stem powder, and the mass ratio of the Dendrobium officinale stem powder to water is 1:(40-80).

[0018] Preferably, the conditions for the water extraction in step S1 are: the extraction temperature is 60-80° C., and the extraction time is 60-120 min.

[0019] Preferably, the complex enzyme in step S2 comprises an enzyme produced by anaerobic Bacillus nubigenes and cellulase; the mass ratio of the enzyme produced by anaerobic Bacillus nubigenes and cellulase is (2-5):1.

[0020] The product enzyme and cellulase of the anaerobic bacillus nubihalogenis used in the present invention are more likely to perform fixed-point enzymatic digestion on the Dendrobium officinale polysaccharide, and have a better enzymatic digestion effect. After enzymatic digestion, ultra-low molecular weight Dendrobium officinale polysaccharide or Dendrobium officinale oligosaccharide is obtained, which has a better scalp care effect. In addition, under the action of enzymatic hydrolysis, the high molecular weight protein in the extract is hydrolyzed into small molecular peptides, which is more conducive to the transdermal absorption of the scalp, thereby enhancing the barrier function of the scalp. At the same time, the high molecular weight protein in the extract is also converted into small molecular protein or small molecular peptide under the action of enzymatic hydrolysis, which is more conducive to the transdermal absorption of the skin.

[0021] Preferably, the added amount of the complex enzyme in step S2 is 0.2-0.6% of the mass of the Dendrobium officinale stem powder.

[0022] Preferably, the enzymatic hydrolysis conditions in step S2 are: the enzymatic hydrolysis temperature is 45-55° C., and the enzymatic hydrolysis time is 3-5 h.

[0023] Preferably, the reagent used to adjust the pH in step S2 to 5.0-6.0 includes at least one of 0.5-5.0% hydrochloric acid solution, 0.5-5.0% nitric acid solution, and 0.5-5.0% sulfuric acid solution.

[0024] Preferably, after the enzymatic hydrolysis in step S2 and before the filtration, the complex enzyme is inactivated, the inactivation temperature is 80-95° C., and the inactivation time is 20-40 min.

[0025] Preferably, the filtration in step S2 is performed using a diatomaceous earth filter.

[0026] Preferably, the filtrate of the fermentation product of the galactosaccharomyces-like bacteria is obtained by fermenting with Trichosporon berreyi.

[0027] The filtrate of the fermentation product of galactosaccharomyces is usually obtained by fermenting Trichosporon, but not all Trichosporon have good efficacy. The present invention screens a large number of strains by fermentation, and finally screens Trichosporon beretianus. The filtrate of the fermentation product of galactosaccharomyces obtained by fermentation has good skin care effect. After being combined with Dendrobium officinale hydrolysate, sodium hyaluronate, pentanediol and hexylene glycol, the obtained scalp care composition has a good effect of regulating skin microecology, which is beneficial to the colonization of beneficial bacteria on the scalp, so that the scalp can better resist exogenous stimulation and enhance the scalp barrier.

[0028] Preferably, the preparation method of the filtrate of the galactose yeast-like bacteria fermentation product is:

[0029] The seed liquid of Trichosporon berreyi is added into the fermentation medium and fermented in a micro-aerobic manner to obtain a crude fermentation liquid; the crude fermentation liquid is sterilized and filtered to obtain a filtrate of a fermentation product of galactose yeast-like bacteria.

[0030] Preferably, in terms of mass percentage, the fermentation medium comprises 1-1.8% highland barley flour, 0.1-0.4% glutinous sorghum flour, 0.8-1.4% yeast extract powder, 0.8-1.8% lactose and water to make up to a total mass percentage of 100%.

[0031] Preferably, the bacterial concentration of the Bereichia spore yeast seed solution is 10 7 -10 9 CFU / mL, the inoculation amount of the Trichosporon berreyi seed solution is 0.8-2.5% (v / v).

[0032] Preferably, the temperature of the microaerobic fermentation is 25-30° C., the time of the microaerobic fermentation is 36-60 h, and the amount of sterile air introduced is 0.05-0.2 vvm.

[0033] Preferably, the sterilization temperature is 80-95°C and the sterilization time is 20-40 minutes.

[0034] Preferably, the filtration is filtration using a diatomaceous earth filter.

[0035] Another aspect of the present invention provides use of the scalp care composition in the preparation of daily chemicals.

[0036] The daily chemicals are any preparations conventionally prepared in the field of hair products, and can be formulated into any one including but not limited to solutions, emulsions, suspensions, pastes, gels, creams, essences, powders, soaps, detergents containing surfactants, oils, powder creams, and sprays.

[0037] Preferably, the scalp care composition is added in an amount of 0.1-50% by mass in daily chemicals.

[0038] Preferably, the daily chemical product of the present invention is selected from any one of scalp care essence, scalp care water, scalp nourishing cream, scalp massage cream, shampoo and conditioner.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] (1) The scalp care composition of the present invention rationally mixes Dendrobium officinale hydrolysate, galactosyl yeast fermentation product filtrate, sodium hyaluronate, pentylene glycol and hexylene glycol, and the obtained composition has the effects of anti-inflammatory, moisturizing, soothing and protecting scalp microecology. The composition of the present invention utilizes the synergistic effect of Dendrobium officinale hydrolysate and galactosyl yeast fermentation product filtrate to better maintain the stability of scalp microecology and enhance the scalp barrier function, and the synergy of sodium hyaluronate, pentylene glycol and hexylene glycol can better resist exogenous stimulation and further promote the health of the scalp and hair growth.

[0041] (2) The 1,2-pentanediol and 1,2-hexanediol used in the present invention can promote the efficient penetration of nutrients in the scalp care composition, and promote the stability of the composition, making it less likely to deteriorate, and better exerting its scalp care effect.

[0042] (3) The scalp care composition of the present invention uses the product enzyme of anaerobic Bacillus nubihalogenis and cellulase to enzymatically digest the active ingredients in Dendrobium candidum, thereby obtaining ultra-low molecular weight Dendrobium candidum polysaccharides or Dendrobium candidum oligosaccharides, which penetrate into the dermis of the skin and have better scalp care effects. The low molecular weight Dendrobium candidum polysaccharides and sodium hyaluronate work together to achieve a synergistic effect, which can effectively relieve scalp inflammation. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 This is the cell migration status of the negative control group at 0h;

[0044] Figure 2Cell migration at 24 h for negative control;

[0045] Figure 3 This is the cell migration status of the experimental group in Example 3 at 0h;

[0046] Figure 4 This is the cell migration status of the experimental group in Example 3 at 24 hours. DETAILED DESCRIPTION

[0047] The present invention is further described below in conjunction with the examples. It should be noted that the following examples are only used to explain the present invention, but not to limit the present invention. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples, if not otherwise specified, can be obtained through commercial channels. Unless otherwise specified, the percentages mentioned in the present invention are all mass percentages.

[0048] The sodium hyaluronate of different molecular weights used in the present invention is all derived from Guangzhou Juwei Microbiology Technology Co., Ltd.; the product enzyme, cellulase and pectinase of Bacillus nubiquitus are derived from Dongguan Juwei New Material Technology Co., Ltd.; the beretian yeast is beretian yeast (AS2.1193), which is derived from Beijing Biobowei Biotechnology Co., Ltd.; the asahish yeast is asahish yeast (bio-85150), which is derived from Beijing Biobowei Biotechnology Co., Ltd.; the spore-coated spore yeast is spore-coated spore yeast (CCTCC M 20221857), which is derived from Dongguan Juwei New Material Technology Co., Ltd.; the brewer's yeast is brewer's yeast (BNCC336054), which is derived from Beijing Beina Chuanglian Biotechnology Research Institute; the beretian yeast seed liquid, the asahish yeast seed liquid, the spore-coated spore yeast seed liquid, and the brewer's yeast seed liquid are prepared by the methods commonly used in the prior art.

[0049] Examples 1-6

[0050] A scalp care composition comprises galactosyl yeast-like bacteria fermentation product filtrate, sodium hyaluronate, Dendrobium officinale enzymatic hydrolysate, pentanediol and hexylene glycol, and the specific preparation steps are as follows.

[0051] 1. Prepare the enzymatic hydrolysate of Dendrobium officinale, the specific steps are as follows:

[0052] S1. Setting the pressure of the extruder to 0.6 MPa and the temperature to 65° C., adding the stem strips of Dendrobium officinale to the extruder and extruding for 120 min, cooling to room temperature, and grinding to obtain Dendrobium officinale stem powder; mixing 1 part of Dendrobium officinale stem powder with 60 parts of water by mass, heating to 70° C., and extracting for 90 min to obtain an extract;

[0053] S2. The pH of the extract was adjusted to 5.5 with a 2.5% hydrochloric acid solution, and a composite enzyme (the mass ratio of the product enzyme of anaerobic Bacillus nubiquitus and cellulase was 3:1) with a mass of 0.4% of the stem powder of Dendrobium officinale was added, and enzymolysis was performed at 50° C. for 4 hours to obtain an enzymolyzate; the enzymolyzate was inactivated by keeping warm at 85° C. for 30 minutes, and filtered through a diatomaceous earth filter. The filtrate obtained was the enzymolyzate of Dendrobium officinale.

[0054] 2. Preparation of galactosyl yeast fermentation product filtrate, the specific steps are as follows:

[0055] Prepare fermentation medium: Mix 1.4% highland barley flour, 0.25% glutinous sorghum flour, 1.1% yeast extract powder, 1.3% lactose and 95.95% water by mass percentage, and sterilize by high pressure. 8 The CFU / mL Beresheet yeast seed liquid was added into the fermentation culture at 1.3% (v / v), and the culture was fermented in a microaerobic manner for 48 hours under the condition of sterile air ventilation volume of 0.12vvm to obtain a crude fermentation liquid; the crude fermentation liquid was then inactivated by keeping warm at 85°C for 30 minutes, and the filtrate obtained after filtering through a diatomaceous earth filter was the filtrate of the fermentation product of the galactosidase-like bacteria.

[0056] 3. The filtrate of the fermentation product of galactosyl yeast, sodium hyaluronate, enzymatic hydrolysate of Dendrobium officinale, pentanediol and hexylene glycol were mixed to prepare a scalp care composition. The content of each component in mass percentage is shown in Table 1.

[0057] Table 1 Content of each component in Examples 1-6

[0058]

[0059] Example 7

[0060] A scalp care composition, the specific preparation steps are as follows.

[0061] 1. Prepare the enzymatic hydrolysate of Dendrobium officinale, the specific steps are as follows:

[0062] S1. Setting the pressure of the extruder to 0.45 MPa and the temperature to 80° C., adding the stem strips of Dendrobium officinale to the extruder and extruding for 90 min, cooling to room temperature, and grinding to obtain Dendrobium officinale stem powder; mixing 1 part of Dendrobium officinale stem powder with 80 parts of water by mass, heating to 60° C., and extracting for 120 min to obtain an extract;

[0063] S2. The pH of the extract was adjusted to 5.0 with a 5% nitric acid solution, and a composite enzyme (the mass ratio of the product enzyme of anaerobic Bacillus nubiquitus and cellulase was 2:1) with a mass of 0.6% of the Dendrobium officinale stem powder was added, and enzymolysis was performed at 55° C. for 3 h to obtain an enzymolyzate; the enzymolyzate was inactivated by keeping warm at 95° C. for 20 min, and filtered through a diatomaceous earth filter. The filtrate obtained was the Dendrobium officinale enzymolyzate.

[0064] 2. Preparation of galactose yeast fermentation product filtrate, the specific method is as follows:

[0065] Prepare fermentation medium: Mix 1% highland barley flour, 0.1% glutinous sorghum flour, 1.4% yeast extract powder, 0.8% lactose and 96.7% water by mass percentage, and sterilize by high pressure. 9 The CFU / mL Beresheet yeast seed liquid was added to the fermentation culture at 0.8% (v / v), and microaerobic fermentation was carried out for 36 hours under a sterile air ventilation volume of 0.2vvm to obtain a crude fermentation liquid; then the crude fermentation liquid was inactivated by keeping warm at 80°C for 40 minutes, and the filtrate obtained after filtering through a diatomaceous earth filter was the filtrate of the galactosidase-like bacteria fermentation product.

[0066] 3. By mass percentage, 89.23% of galactosyl yeast fermentation product filtrate, 4.52% of sodium hyaluronate with a molecular weight of 950±50Da, 3.25% of Dendrobium officinale enzymatic hydrolysate, 1.2% of pentanediol and 1.8% of hexanediol were mixed evenly to obtain a product.

[0067] Example 8

[0068] A scalp care composition, the specific preparation steps are as follows.

[0069] 1. Prepare the enzymatic hydrolysate of Dendrobium officinale, the specific steps are as follows:

[0070] S1. Setting the pressure of the extruder to 1.0 MPa and the temperature to 50° C., adding the stem strips of Dendrobium officinale to the extruder and extruding for 150 min, cooling to room temperature, and grinding to obtain Dendrobium officinale stem powder; mixing 1 part of Dendrobium officinale stem powder with 40 parts of water by mass, heating to 80° C., and extracting for 60 min to obtain an extract;

[0071] S2. The pH of the extract was adjusted to 6.0 with 0.5% nitric acid solution, and a composite enzyme (the mass ratio of the product enzyme of anaerobic Bacillus nubifolia and cellulase was 5:1) with the mass of 0.2% of Dendrobium officinale stem powder was added, and enzymolysis was performed at 45° C. for 5 hours to obtain an enzymolyzate; the enzymolyzate was inactivated by keeping warm at 80° C. for 40 minutes, and the filtrate was filtered through a diatomaceous earth filter to obtain the Dendrobium officinale enzymolyzate.

[0072] 2. Preparation of galactosyl yeast fermentation product filtrate, the specific steps are as follows:

[0073] Prepare fermentation medium: Mix 1.8% highland barley flour, 0.4% glutinous sorghum flour, 0.8% yeast extract powder, 1.8% lactose and 95.2% water by mass percentage, and sterilize by high pressure. 7 The CFU / mL Beresheet yeast seed liquid is added to the fermentation culture at 2.5% (v / v), and the culture is fermented in a microaerobic manner for 60 hours under a sterile air ventilation volume of 0.05 vvm to obtain a crude fermentation liquid; the crude fermentation liquid is then inactivated by keeping warm at 95°C for 20 minutes, and the filtrate obtained after filtering through a diatomaceous earth filter is a filtrate of a galactosidase-like bacteria fermentation product.

[0074] 3. Evenly mix 92.99% of galactosyl yeast fermentation product filtrate, 2.88% of sodium hyaluronate with a molecular weight of 750±50Da, 1.13% of Dendrobium officinale enzymatic hydrolysate, 1.7% of pentanediol and 1.3% of hexanediol to obtain the product.

[0075] Comparative Examples 1-5

[0076] A scalp care composition comprises any four of galactosyl yeast fermentation product filtrate, sodium hyaluronate, Dendrobium officinale enzymatic hydrolysate, pentanediol and hexylene glycol, and the content of each component is as shown in the following table in terms of mass percentage.

[0077] The preparation methods of the enzymatic hydrolysate of Dendrobium candidum and the preparation methods of the filtrate of the fermentation product of Galacto-saccharomyces-like bacteria in Comparative Examples 1-5 are consistent with those in Examples 1-6. The difference between Comparative Examples 1-5 and Example 3 is that Comparative Examples 1-5 lack one of the components in Example 3, and the content of the other components is increased in a corresponding proportion.

[0078] Table 2 Comparative Examples 1-5 Component Contents

[0079]

[0080] Comparative Example 6

[0081] A scalp care composition is basically the same as Example 3, the only difference being that the filtrate of the fermentation product of galactosyl yeast-like bacteria is replaced with deionized water of the same mass fraction.

[0082] Comparative Example 7

[0083] A scalp care composition is basically the same as Example 3, the only difference being that the sodium hyaluronate with a molecular weight of 800±50Da is replaced by sodium hyaluronate with a molecular weight of 5000±100Da.

[0084] Comparative Example 8

[0085] A scalp care composition is basically the same as Example 3, the only difference being that in the preparation method of the enzymatic hydrolysate of Dendrobium officinale, no puffing is performed in step S1. The preparation method of the enzymatic hydrolysate of Dendrobium officinale in this comparative example is as follows:

[0086] S1. Grinding the stem strips of Dendrobium officinale to obtain Dendrobium officinale stem powder; mixing 1 part of Dendrobium officinale stem powder with 60 parts of water by mass, heating to 70° C., and extracting for 90 minutes to obtain an extract;

[0087] S2. The pH of the extract was adjusted to 5.5 with a 2.5% hydrochloric acid solution, and a composite enzyme (the mass ratio of the product enzyme of anaerobic Bacillus nubiquitus and cellulase was 3:1) with a mass of 0.4% of the stem powder of Dendrobium officinale was added, and enzymolysis was performed at 50° C. for 4 hours to obtain an enzymolyzate; the enzymolyzate was inactivated by keeping warm at 85° C. for 30 minutes, and filtered through a diatomaceous earth filter. The filtrate obtained was the enzymolyzate of Dendrobium officinale.

[0088] Comparative Example 9

[0089] A scalp care composition is basically the same as Example 3, the only difference being that in the preparation method of the enzymatic hydrolysate of Dendrobium officinale, the compound enzyme in step S2 is a mixture of pectinase and cellulase in a mass ratio of 3:1. The preparation method of the enzymatic hydrolysate of Dendrobium officinale in this comparative example is as follows:

[0090] S1. Setting the pressure of the extruder to 0.6 MPa and the temperature to 65° C., adding the stem strips of Dendrobium officinale into the extruder for extrusion, cooling to room temperature, and grinding to obtain Dendrobium officinale stem powder; mixing 1 part of Dendrobium officinale stem powder with 60 parts of water by mass, heating to 70° C., and extracting for 90 min to obtain an extract;

[0091] S2. The pH of the extract was adjusted to 5.5 with a 2.5% hydrochloric acid solution, and a composite enzyme (the mass ratio of pectinase and cellulase was 3:1) with a mass of 0.4% of Dendrobium officinale stem powder was added, and enzymolysis was performed at 50° C. for 4 h to obtain an enzymolysis product; the enzymolysis product was inactivated by keeping warm at 85° C. for 30 min, and filtered through a diatomaceous earth filter to obtain a filtrate that was a Dendrobium officinale extract.

[0092] Comparative Example 10

[0093] A scalp care composition is basically the same as Example 3, the only difference being that in the preparation method of the Dendrobium officinale extract, step S2 does not perform enzymatic hydrolysis. The preparation method of the Dendrobium officinale extract of this comparative example is:

[0094] S1. Setting the pressure of the extruder to 0.6 MPa and the temperature to 65° C., adding the stem strips of Dendrobium officinale into the extruder for extrusion, cooling to room temperature, and grinding to obtain Dendrobium officinale stem powder; mixing 1 part of Dendrobium officinale stem powder with 60 parts of water by mass, heating to 70° C., and extracting for 6 hours to obtain an extract;

[0095] S2. The extract is kept at 85° C. for 30 min to be inactivated, and the filtrate obtained after filtering through a diatomaceous earth filter is the Dendrobium officinale extract.

[0096] Comparative Example 11

[0097] A scalp care composition is substantially the same as that of Example 3, except that the filtrate of the fermentation product of Galactomyces spp. is replaced by the filtrate of the fermentation product of Galactomyces spp. fermented by Trichosporon asahii.

[0098] The preparation method of the galactose yeast-like bacteria fermentation product filtrate of this comparative example is:

[0099] Prepare fermentation medium: Mix 1.4% highland barley flour, 0.25% glutinous sorghum flour, 1.1% yeast extract powder, 1.3% lactose and 95.95% water by mass percentage, and sterilize by high pressure. 8 The CFU / mL Asahis spore seed liquid was added to the fermentation culture at 1.3% (v / v), and the culture was fermented in a microaerobic manner for 48 hours under a sterile air ventilation volume of 0.12 vvm to obtain a crude fermentation liquid; the crude fermentation liquid was then inactivated by keeping warm at 85°C for 30 minutes, and the filtrate obtained after filtering through a diatomaceous earth filter was the filtrate of the galactosidase-like bacteria fermentation product.

[0100] Comparative Example 12

[0101] A scalp care composition is basically the same as Example 3, the only difference being that the filtrate of the fermentation product of Galactomyces cerevisiae is replaced by the filtrate of the fermentation product of Galactomyces cerevisiae fermented by Capsella cingulata.

[0102] The preparation method of the galactose yeast-like bacteria fermentation product filtrate of this comparative example is:

[0103] Prepare fermentation medium: Mix 1.4% highland barley flour, 0.25% glutinous sorghum flour, 1.1% yeast extract powder, 1.3% lactose and 95.95% water by mass percentage, and sterilize by high pressure. 8The CFU / mL of Saccharomyces cerevisiae seed liquid was added to the fermentation culture at 1.3% (v / v), and microaerobic fermentation was carried out for 48 hours under sterile air ventilation volume of 0.12vvm to obtain a crude fermentation liquid; then the crude fermentation liquid was inactivated by keeping warm at 85°C for 30 minutes, and the filtrate obtained after filtering through a diatomaceous earth filter was the filtrate of the fermentation product of the galactosidase-like bacteria.

[0104] Comparative Example 13

[0105] A scalp care composition is substantially the same as that of Example 3, except that the filtrate of the fermentation product of galactosaccharomyces-like bacteria is replaced with the filtrate of the fermentation product of galactosaccharomyces-like bacteria fermented by Saccharomyces cerevisiae.

[0106] The preparation method of the galactose yeast-like bacteria fermentation product filtrate of this comparative example is:

[0107] Prepare fermentation medium: Mix 1.4% highland barley flour, 0.25% glutinous sorghum flour, 1.1% yeast extract powder, 1.3% lactose and 95.95% water by mass percentage, and sterilize by high pressure. 8 CFU / mL of brewer's yeast seed liquid is added to the fermentation culture at 1.3% (v / v), and microaerobic fermentation is carried out for 48 hours under sterile air ventilation volume of 0.12vvm to obtain a crude fermentation liquid; then the crude fermentation liquid is inactivated by keeping warm at 85°C for 30 minutes, and the filtrate obtained after filtering through a diatomaceous earth filter is the filtrate of the galactosidase-like bacteria fermentation product.

[0108] Comparative Example 14

[0109] A scalp care composition is substantially the same as Example 3, except that pentanediol and hexylene glycol are replaced with 1,2-butanediol.

[0110] Test Example 1: Soothing Performance Test

[0111] In this study, LPS-induced mouse macrophage RAW264.7 was used as an in vitro inflammatory cell model to measure the relative expression of inflammatory factor (TNF-ɑ) mRNA and the amount of nitric oxide (NO) to evaluate whether the test substance has a soothing effect.

[0112] Test sample: The scalp care compositions of Examples 1-8 and Comparative Examples 1-14 were added to DMEM culture medium and diluted to a solution with a mass concentration of 2% of the scalp care composition.

[0113] Test steps: Take RAW264.7 cells in logarithmic phase and inoculate them into culture flasks. Collect and count the RAW264.7 cells grown to 90%. Dilute the cell suspension with DMEM medium (hereinafter referred to as medium) according to the cell number of 2.0×10 5Each well was inoculated into a 6-well plate and placed at 37°C with 5% CO 2 The cells were cultured in an incubator for 24 h to allow the cells to spread normally. The original culture medium in the well plate was then discarded, 2 mL of fresh culture medium was added to the negative control group, 1 mL of culture medium and 1 mL of 5 μg / mL LPS solution was added to the model control group, and 1 mL of 5 μg / mL LPS solution and 1 mL of test sample solution were added to the experimental group. The well plate was placed at 37°C and 5% CO 2 The cells were cultured in an incubator for 6 h for induction. The original culture medium in the wells was then discarded, 2 mL of fresh culture medium was added to each well, and the cells were placed at 37°C and 5% CO 2 Culture in an incubator for 24 h.

[0114] Relative expression of TNF-ɑ mRNA: After collecting cells, RNA was extracted and reverse transcribed into cDNA. Fluorescence quantitative PCR was used to determine the expression of TNF-ɑ. The expression of TNF-ɑ mRNA in the negative control group was set as 1.00, and the relative expression of TNF-ɑ mRNA in other groups of cells was calculated.

[0115] Relative content of NO: The cells in each group were crushed and the supernatant was collected. The NO content in the supernatant was tested according to the instruction manual of the nitric oxide detection kit. The NO content of the model control group was taken as 100%, and the relative content of NO in the cells of other groups was calculated.

[0116] The test results are shown in the following table:

[0117] Table 3 Soothing performance test results

[0118]

[0119]

[0120] From the above results, it can be seen that compared with the negative control group, the expression of TNF-ɑmRNA in the macrophages of the model control group increased relatively after being stimulated by LPS, that is, the inflammatory factor TNF-ɑ produced by the cells increased to 2.41 times; the NO content produced by the cells also increased from 6.63% to 100%.

[0121] Using the cells of Examples 1-8, the TNF-ɑ mRNA expression level was reduced to 1.76 to 1.95 times that of the negative control group, and the NO content was relatively reduced by 71.51 to 79.45%, indicating that the scalp care composition of the embodiments of the present invention has a good effect of reducing inflammation and soothing.

[0122] The TNF-ɑ mRNA expression of the cells using comparative examples 1-5 was 2.26 to 2.69 times that of the negative control group, and the NO content was only reduced to 83.45 to 99.62%, indicating that the lack of any component in the scalp care composition will lead to a poor soothing effect. In particular, comparative example 1 lacks the filtrate of the fermentation product of galactosyl yeast-like bacteria, and the scalp care composition formed after the corresponding proportion of other components is increased, pentanediol and butanediol are increased too much, and the polyols in the system are too much, resulting in increased cytotoxicity of the scalp care composition, and the final product shows a worse soothing effect. For comparative example 2, the soothing effect of the Dendrobium officinale hydrolysate prepared by the preparation method of the present invention is greatly reduced in the absence of the filtrate of the fermentation product of galactosyl yeast-like bacteria, indicating that the two have a synergistic effect.

[0123] The soothing effect of Examples 1-8 is also better than that of Comparative Examples 6 and 11-13. The expression of TNF-ɑmRNA in cells of Comparative Examples 6 and 11-13 is reduced to 2.08 to 2.35 times that of the negative control group, and the NO content is only reduced to 83.35 to 95.24%. It is shown that whether the galactose yeast-like bacteria fermentation product filtrate is added or not and the fermentation strain of the galactose yeast-like bacteria fermentation product filtrate in the present invention have an effect on the soothing effect of the final composition. The effect of the galactose yeast-like bacteria fermentation product filtrate obtained by fermenting the Beleissporum yeast used in the present invention is better than that of other strains of the same type.

[0124] The soothing effects of Examples 1-8 are better than those of Comparative Example 7, indicating that the 600-1000 Da sodium hyaluronate used in the present invention is more conducive to the soothing effect of the composition compared with sodium hyaluronate with a larger molecular weight.

[0125] The soothing effects of Examples 1-8 are better than those of Comparative Examples 8-10, indicating that the preparation process of the enzymatic hydrolysate of Dendrobium officinale of the present invention has a beneficial effect on the soothing effect of the final composition.

[0126] The soothing effects of Examples 1-8 are better than those of Comparative Examples 11-13, indicating that the fermentation product filtrate of Trichosporon berberis used in the present invention has a better soothing effect on the scalp.

[0127] The soothing effect of Examples 1-8 is better than that of Comparative Example 14, indicating that the 1,2-pentanediol and 1,2-hexanediol used in the present invention can synergistically enhance the effect with other ingredients to promote the soothing effect.

[0128] Test Example 2: Repair Performance Test

[0129] Cell migration refers to the movement of cells after receiving migration signals or being stimulated by certain substances. It is manifested as the extension of pseudopodia of the cell head, the establishment of new adhesions, and the contraction of the cell tail in time and space. The migration ability of cells is also an important indicator in skin (scalp) repair. The effect of samples on the migration ability of fibroblasts is tested through in vitro experiments to evaluate whether the samples have the effect of promoting skin (scalp) repair.

[0130] Test sample: the scalp care composition of Examples 1-8 and Comparative Examples 1-14, diluted with DMEM culture medium to a solution with a mass concentration of 2%.

[0131] Test steps: Take fibroblasts (L929 cells) in logarithmic phase and inoculate them into culture flasks. Collect and count L929 cells that have grown to 90%. Dilute the cell suspension with DMEM medium (hereinafter referred to as medium) according to the cell number of 1.0×10 4 Each well was inoculated in a 48-well plate and placed at 37°C with 5% CO 2 Culture in an incubator for 24 h to allow the cells to spread normally.

[0132] Take out the culture plate, discard the original culture medium in the well, draw a cross line with the tip of the pipette, add 1 mL of culture medium to the negative control group, add 1 mL of test sample solution to the sample group, and place at 37°C and 5% CO 2 The cells were cultured in an incubator for 24 hours. After the culture was completed, the cells were photographed using an electron microscope. The cell migration at the scratch of the negative control group and each test sample group was photographed using an electron microscope, and the cell migration rate was calculated. The results are shown in the following table:

[0133] Table 4 Repair performance test results

[0134] Group Cell migration rate (%) Group Cell migration rate (%) Negative control group 8.46 Comparative Example 4 36.53 Example 1 41.45 Comparative Example 5 35.37 Example 2 42.25 Comparative Example 6 28.95 Example 3 46.74 Comparative Example 7 34.62 Example 4 44.19 Comparative Example 8 35.26 Example 5 40.56 Comparative Example 9 35.86 Example 6 44.93 Comparative Example 10 31.13 Example 7 38.04 Comparative Example 11 30.13 Example 8 41.31 Comparative Example 12 33.68 Comparative Example 1 3.57 Comparative Example 13 27.35 Comparative Example 2 34.53 Comparative Example 14 37.04 Comparative Example 3 30.45 / /

[0135] From the above results, it can be seen that the cell migration rate of the negative control group is 8.46%, and the cell migration rate of the experimental group using Examples 1-8 is 40.56%-46.74%. Compared with the negative control group, the migration rate of the cells using Examples 1-8 of the present invention is increased. This shows that the scalp care composition of the present invention can better promote cell migration, that is, the composition of the present invention has a better repair effect.

[0136] It can be seen from the fact that the repairing effect of Examples 1-8 is better than that of Comparative Examples 1-5 that, in the scalp care composition, if any of the components of the filtrate of the fermentation product of galactosyl yeast, sodium hyaluronate, the enzymatic hydrolysate of Dendrobium officinale, pentanediol and butylene glycol are missing, the repairing effect of the composition will be poor.

[0137] The cell migration rate of Comparative Example 1 is lower than that of the negative control group. This is because too much pentanediol and butanediol are added to the scalp care composition of Comparative Example 1, and too much polyol in the system increases the cytotoxicity of the scalp care composition and stagnates cell migration.

[0138] The repairing effects of Examples 1-8 are better than those of Comparative Examples 6 and 11-13, indicating that the galactosaccharomyces cerevisiae fermentation product filtrate and the fermentation strains of the galactosaccharomyces cerevisiae fermentation product filtrate used in the present invention have a better influence on the repairing effect of the final composition.

[0139] The repairing effects of Examples 1-8 are better than those of Comparative Example 7, indicating that the preferred molecular weight of hyaluronic acid of the present invention can better promote the repairing effect of the final product.

[0140] The repairing effect of Examples 1-8 is better than that of Comparative Examples 8-10, indicating that the preparation process of the enzymatic hydrolysis of Dendrobium officinale has a great influence on the repairing effect of the final composition, and the enzymatic hydrolyzate of Dendrobium officinale obtained by the preparation method adopted in the present invention has a better effect of promoting repairing.

[0141] Test Example 3: Scalp Microecology Regulation Performance Test

[0142] There are many kinds of microorganisms distributed on the human scalp, mainly including Staphylococcus sp., Propionibacterium sp. and Malassezia, and the density of microbial cells is about 10 3 -10 5 CFU / mm 2 . Staphylococcus epidermidis is the main component of the normal symbiotic flora of healthy people's skin, which is beneficial to the balance of scalp microecology. It not only resists potential pathogenic microorganisms, but also inhibits the overgrowth of existing conditional pathogens. Propionibacterium acnes lives in hair follicles and sebaceous glands and is one of the causes of acne. Malassezia furfur is an important factor in inducing or aggravating diseases such as seborrheic dermatitis, hair loss, pityriasis capitis, folliculitis, pruritus, and tinea capitis.

[0143] This test explores the performance of the test samples in regulating the scalp microecology by detecting the inhibitory effect of the test samples on Staphylococcus, Propionibacterium and Malassezia furfur. According to 2.1.8.3 Minimum inhibitory concentration test (agar dilution method) in the 2022 edition of the "Technical Specifications for Disinfection" of the Ministry of Health, the samples prepared in Examples 1-8 of the present invention and Comparative Examples 1-14 were tested for minimum inhibitory concentration.

[0144] Test strains: Staphylococcus epidermidis ATCC 12228; Malassezia furfur ATCC 44344, both from Guangdong Institute of Microbiology. The test results are as follows:

[0145] Table 5 Regulating scalp microecological performance test results

[0146]

[0147]

[0148] The test results show that the scalp care composition of the embodiment of the present invention has an antibacterial concentration of >100,000 mg / kg against Staphylococcus epidermidis, and exhibits a certain inhibitory effect on Propionibacterium acnes and Malassezia furfur, indicating that the scalp care composition of the embodiment of the present invention inhibits the colonization of normal skin flora, inhibits the reproduction of harmful flora, and has a good effect of regulating the microecology.

[0149] Test Example 4: Human body repair performance test

[0150] TEWL refers to the amount of water lost per unit area per unit time, and the unit is: g / hm 2 TEWL is a very important parameter for evaluating the function of the skin's moisture barrier and has been widely recognized internationally. The more intact the skin's moisture barrier is, the less likely the skin's moisture is to be lost, and the lower the TEWL value for skin moisture loss is. During the development of hair products, by testing the TEWL value for skin moisture loss, it is possible to evaluate whether the skin's protective function has been damaged and whether the product has the function of repairing the scalp barrier.

[0151] Test samples: base serum, serum containing scalp care composition. The formulas of base serum and serum containing scalp care composition are as follows:

[0152] Table 6 Essence composition and content

[0153]

[0154] The preparation method of the essence containing the scalp care composition is as follows:

[0155] (1) Add the materials of component A into the main pot, mix well, and keep warm at 80°C for 10 minutes;

[0156] (2) Cool the main pot to 60°C, add component B into the main pot and mix well;

[0157] (3) Cool the main pot to 50°C, add the materials of component C into the main pot and mix evenly;

[0158] (4) Cool the main pot to 50° C., add the ingredients of component D into the main pot, mix well, and obtain 22 sets of conditioning essences.

[0159] The formula and preparation method of the matrix essence are basically the same as those of the essence containing the scalp care composition, the only difference being that the scalp care composition in the above formula C component is replaced with deionized water of the same mass.

[0160] Test steps: 115 subjects with damaged scalp barrier and TEWL value of 20g / hm were selected 2 The above volunteers were randomly divided into 23 groups, and each group used a set of essence. Before using the test samples, the TEWL value of the scalp was measured in the head test area. The volunteers used the test samples according to the groups, once a day. After two weeks of use, the TEWL value of the scalp was measured again in the head test area. The change rate was calculated based on the TEWL values ​​before and after use, and the test results were averaged. The test results are shown in the following table.

[0161] Average: Average Where: x n represents the individual parameter detection value, and n represents the number of valid data.

[0162] Difference: in, It indicates the average value of the parameters detected at the nth time point after use. It represents the average value of the parameters tested before use.

[0163] Rate of change: Indicates the degree of change in the mean relative to the initial value.

[0164] Table 7 Human body repair performance test results

[0165] Group TEWL change rate (%) Group TEWL change rate (%) Matrix Essence 7.26 Comparative Example 4 0.6 Example 1 -7.8 Comparative Example 5 -0.8 Example 2 -9.8 Comparative Example 6 -1.5 Example 3 -10.5 Comparative Example 7 -2.5 Example 4 -8.6 Comparative Example 8 -2.6 Example 5 -6.2 Comparative Example 9 -1.8 Example 6 -6.7 Comparative Example 10 -3.2 Example 7 -5.2 Comparative Example 11 -1.6 Example 8 -4.6 Comparative Example 12 -2.4 Comparative Example 1 8.0 Comparative Example 13 -3.5 Comparative Example 2 -1.3 Comparative Example 14 -3.0 Comparative Example 3 -0.4 / /

[0166] Compared with the matrix essence, after using the essence containing Examples 1-8 of the present invention, the TEWL value of the scalp is reduced and the scalp barrier is strengthened. This is because the scalp care composition of the embodiments of the present invention can relieve the production of inflammatory factors by scalp inflammatory cells, regulate the scalp microecology, promote cell migration, and promote barrier repair, so that when the final product is used in the human body, the human scalp barrier is repaired and water loss is reduced.

Claims

1. A scalp care composition, characterized in that: The invention comprises the following components by mass percentage: 0.5-6.0% sodium hyaluronate, 0.2-4.0% enzymatic hydrolysate of Dendrobium officinale, 1.0-3.0% pentanediol, 1.0-3.0% hexanediol and galactose yeast-like bacteria fermentation product filtrate supplemented to a total mass percentage of 100%.

2. The scalp care composition according to claim 1, characterized in that The preparation method of the Dendrobium officinale hydrolysate is as follows: S1, puffing, grinding, and extracting the stems of Dendrobium officinale to obtain a water extract of Dendrobium officinale; S2, adjusting the pH of the Dendrobium officinale water extract to 5-6, adding a complex enzyme, performing enzymolysis, and filtering, and the obtained filtrate is the Dendrobium officinale enzymolysate.

3. The scalp care composition according to claim 2, characterized in that In step S2, the complex enzyme is composed of the product enzyme of anaerobic Bacillus nubivorus and cellulase in a mass ratio of 2-5:

1.

4. The scalp care composition according to claim 2, characterized in that The puffing conditions in step S1 are as follows: pressure of 0.45-1.0 MPa, temperature of 50-80° C., and duration of 90-150 min.

5. The scalp care composition according to claim 2, characterized in that Step S1: the Dendrobium officinale stem strips are puffed and ground into powder to obtain Dendrobium officinale stem powder, wherein the mass ratio of the Dendrobium officinale stem powder to water is 1:(40-80); In step S2, the amount of the complex enzyme added is 0.2-0.6% of the mass of the Dendrobium officinale stem powder; The temperature of the enzymolysis in step S2 is 45-55° C., and the duration of the enzymolysis is 3-5 h.

6. The scalp care composition according to claim 1, characterized in that The preparation method of the galactose yeast-like bacteria fermentation product filtrate is as follows: The seed liquid of Trichosporon berreyi is added to the culture medium, and the crude fermentation liquid is obtained by microaerobic fermentation. The crude fermentation liquid is sterilized and filtered to obtain the filtrate of the fermentation product of the galactose yeast-like bacteria.

7. The scalp care composition according to claim 6, characterized in that The temperature of the microaerobic fermentation is 25-30° C., the time of the microaerobic fermentation is 36-60 hours, and sterile air is continuously introduced during the microaerobic fermentation, and the amount of sterile air introduced is 0.05-0.2 vvm.

8. The scalp care composition according to claim 6, characterized in that The bacterial concentration of the Berejikal yeast seed solution is 10 7 -10 9 CFU / mL, the inoculation amount of the Beleucosporium yeast seed solution is 0.8-2.5% (v / v); The formula of the culture medium is as follows: in terms of mass percentage, it comprises 1-1.8% highland barley flour, 0.1-0.4% glutinous sorghum flour, 0.8-1.4% yeast extract powder, 0.8-1.8% lactose and water supplemented to a total mass percentage of 100%.

9. The scalp care composition according to claim 1, characterized in that The molecular weight of the sodium hyaluronate is 600-1000Da, the pentanediol is 1,2-pentanediol, and the hexanediol is 1,2-hexanediol.

10. Use of the scalp care composition according to any one of claims 1 to 9 in the preparation of daily chemicals.

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