Dressing composition containing NK cell exosomes for repairing sensitive skin and preparation method thereof

Abstract

The dressing composition containing NK cell exosomes for repairing sensitive skin belongs to the field of cosmetic technology, including NK cell exosomes, ceramide, alpine edelweiss, ektoin, ergothioneine pine mushroom extract, 1,2-pentanediol, glycerol, hydrolyzed sodium hyaluronate, disodium EDTA, water, mannitol, trehalose; the preparation method of the dressing composition containing NK cell exosomes for repairing sensitive skin includes three steps. The finished product obtained by the present invention through a variety of raw materials and corresponding preparation methods is suitable for skin sensitivity problems caused by various reasons. The composition has an innovative multi-dimensional three-dimensional mechanism, from the inside to the outside, fundamentally solving the problems of skin sensitivity and redness; using fresh technology to store, ensure higher activity, fundamentally solve and quickly repair sensitive skin, while supplementing nutrients for the basal cells of the skin, soothing the skin state, and improving the problems of dry and rough skin and pigmentation, which can effectively play a good therapeutic role on sensitive skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a dressing composition containing NK cell exosomes for repairing sensitive skin and a preparation method thereof. Background Art

[0002] Affected by the environment and various pressures, many people's facial skin is in a sub-healthy state or damaged in today's society. Sensitive skin in dermatology refers to skin problems such as varying degrees of discomfort when stimulated by physical, chemical, mental and other factors. Sensitive skin has no specific allergens. The biggest cause is damage to the skin barrier. When slightly stimulated by the outside, it will cause sensitivity and discomfort, and may cause fever, tingling, itching and tightness. In severe cases, it is also accompanied by erythema and desquamation. Sensitive skin is divided into primary and secondary, of which more than 70% of sensitive skin is secondary, most of which are caused by topical acne removal, whitening, anti-allergic drugs, improper skin care (excessive cleaning, exfoliation, whitening and freckle removal), etc., as well as the highly sensitive state associated with skin diseases. Whether it is primary or secondary sensitive skin, skin barrier damage is the main link in the occurrence of sensitive skin.

[0003] Currently, glucocorticoids are mainly used to treat skin allergies. However, long-term use of this method can easily lead to side effects such as skin atrophy, capillary dilation, acne pigmentation abnormalities and photosensitivity. At the same time, allergies are prone to recurrence after hormonal drugs are discontinued, which will have a more serious impact on patients' lives and may even cause serious, difficult-to-treat mental illnesses. Therefore, it is crucial to find a safe and effective way to improve skin allergies. Summary of the invention

[0004] In order to overcome the existing treatment methods for skin allergies, which have the disadvantages as described in the background due to technical limitations, the present invention provides a finished product prepared by using NK cell exosomes, ceramide, alpine edelweiss, ectoine, ergothioneine matsutake extract, etc. as raw materials, combined with corresponding processes, which can repair the skin barrier, maintain the water-oil balance, fundamentally improve the skin condition, improve problems such as dry and rough skin, and effectively play a good therapeutic role on sensitive skin. It contains a dressing composition and a preparation method for repairing sensitive skin containing NK cell exosomes.

[0005] The technical solution adopted by the present invention to solve its technical problem is: A dressing composition containing NK cell exosomes for repairing sensitive skin, comprising NK cell exosomes, ceramide, alpine edelweiss, ectoine, ergothioneine agaricus extract, 1,2-pentanediol, glycerol, hydrolyzed sodium hyaluronate, disodium EDTA, water, mannitol, and trehalose; the proportion of NK cell exosomes is 2%-4%, the proportion of ceramide is 4%-6%, the proportion of alpine edelweiss is 3.5%-6%, the proportion of ectoine is 2%-4%, the proportion of ergothioneine agaricus extract is 2%-4%, the proportion of 1,2-pentanediol is 7%-9%, the proportion of glycerol is 2%-4%, the proportion of hydrolyzed sodium hyaluronate is 0.5%-1.5%, the proportion of disodium EDTA is 0.15%-0.4%, the proportion of water is 8%-70%, the proportion of mannitol is 5%-7%, and the proportion of trehalose is 0.5%-1.5%. The preparation method of a NK cell exosome dressing composition for repairing sensitive skin comprises the following steps: S1: adding ceramide, alpine edelweiss, ectoine, ergothioneine matsutake extract, glycerin, hydrolyzed sodium hyaluronate, water, and NK cell exosomes into a beaker in proportion, placing the beaker in a water bath, and then stirring the raw materials in the beaker with an electric stirring device until they are dissolved and transparent to obtain a phase A finished product; S2: mixing mannitol and trehalose in proportion and dispensing them into 5 ml ampoules, and placing the ampoules in a freeze dryer to prepare freeze-dried powder to obtain a phase B finished product; S3: packaging the phase A finished product and the phase B finished product separately. During application, the phase A finished product and the phase B finished product are mixed in a ratio of 10-20:0.5-1.5, and then applied to the affected area to treat sensitive skin.

[0006] Furthermore, the proportion of NK cell exosomes is 3%, the proportion of ceramide is 5%, the proportion of alpine edelweiss is 5%, the proportion of ectoine is 3%, the proportion of ergothioneine matsutake extract is 3%, the proportion of 1,2-pentanediol is 8%, the proportion of glycerol is 3%, the proportion of hydrolyzed sodium hyaluronate is 1%, the proportion of disodium EDTA is 0.3%, the proportion of water is 61.7%, the proportion of mannitol is 6%, and the proportion of trehalose is 1%.

[0007] Furthermore, the temperature of the water bath is 75-85°C, preferably 80°C.

[0008] Furthermore, the stirring rod of the electric stirring device has a rotation speed of 500-600 revolutions per minute, preferably 520 revolutions per minute.

[0009] Compared with the prior art, the present invention has the following beneficial effects: the finished product obtained by the present invention through multiple raw materials and corresponding preparation methods is suitable for skin sensitivity problems caused by various reasons, and the composition has an innovative multi-dimensional three-dimensional mechanism, which fundamentally solves the problems of skin sensitivity and redness from the inside out; it uses fresh technology for storage to ensure higher activity, and while fundamentally solving and quickly repairing sensitive skin, it replenishes nutrients for the basal cells of the skin, soothes the skin condition, and at the same time improves problems such as dry, rough skin and pigmentation, and can effectively play a good therapeutic role on sensitive skin.

[0010] Figure content Figure 1 It is a schematic diagram of the process of preparing the NK cell exosome dressing composition for repairing sensitive skin of the present invention. DETAILED DESCRIPTION

[0011] Figure 1 As shown, the dressing composition containing NK cell exosomes for repairing sensitive skin includes NK cell exosomes, ceramide, alpine edelweiss, ectoine, ergothioneine agaricus extract, 1,2-pentanediol, glycerol, hydrolyzed sodium hyaluronate, disodium EDTA, water, mannitol, and trehalose. Example 1: The ratio of NK cell exosomes is 2.5%, the ratio of ceramide is 4%, the ratio of alpine edelweiss is 6%, the ratio of ectoine is 2.5%, the ratio of ergothioneine agaricus extract is 3%, the ratio of 1,2-pentanediol is 8%, the ratio of glycerol is 2%, the ratio of hydrolyzed sodium hyaluronate is 0.5%, the ratio of disodium EDTA is 0.3%, the ratio of water is 64.2%, the ratio of mannitol is 6%, and the ratio of trehalose is 1%. Example 2: The ratio of NK cell exosomes is 3%, the ratio of ceramide is 5%, the ratio of alpine edelweiss is 5%, the ratio of ectoine is 3%, the ratio of ergothioneine agaric extract is 3%, the ratio of 1,2-pentanediol is 8%, the ratio of glycerol is 3%, the ratio of hydrolyzed sodium hyaluronate is 1%, the ratio of disodium EDTA is 0.3%, the ratio of water is 61.7%, the ratio of mannitol is 6%, and the ratio of trehalose is 1%. Example 3: The ratio of NK cell exosomes is 4%, the ratio of ceramide is 6%, the ratio of alpine edelweiss is 6%, the ratio of ectoine is 2%, the ratio of ergothioneine agaric extract is 4%, the ratio of 1,2-pentanediol is 7%, the ratio of glycerol is 4%, the ratio of hydrolyzed sodium hyaluronate is 1.5%, the ratio of disodium EDTA is 0.4%, the ratio of water is 58.6%, the ratio of mannitol is 5%, and the ratio of trehalose is 1.5%.

[0012] Figure 1As shown, the preparation method of the NK cell exosome dressing composition for repairing sensitive skin includes the following steps: S1: adding ceramide, alpine edelweiss, ectoine, ergothioneine pine mushroom extract, glycerin, hydrolyzed sodium hyaluronate, water, and NK cell exosomes to a beaker in proportion, placing the beaker in a water bath, and then stirring the raw materials in the beaker by an electric stirring device until they are dissolved and transparent to prepare a phase A finished product; the temperature of the water bath is 75-85°C, preferably 80°C. S2: mixing mannitol and trehalose in proportion and dispensing them into 5ml ampoules, and placing the ampoules in a freeze dryer to prepare freeze-dried powder to obtain a phase B finished product; the stirring rod of the electric stirring device rotates at a speed of 500-600 revolutions per minute, preferably 520 revolutions. S3: Package the finished products of phase A and phase B separately. During application, mix the finished products of phase A and phase B in a ratio of 10-20:0.5-1.5 respectively, and then apply them to the affected area to treat sensitive skin.

[0013] Figure 1As shown, in the present invention, the functions of each raw material are as follows. (1) NK cell exosomes: Cells are factories responsible for the production and transmission of molecules. Exosomes (vesicles) are "messengers" between cells. They have natural targeting and can accurately carry lipids, proteins, mRNA, microRNA and other components into aging and damaged cells, playing a role in regeneration, conditioning and repairing damaged cells. Therefore, exosome skin care technology is considered to be a precise biological skin care technology at the cellular level. It uses the repair and regeneration function of exosomes to penetrate into the skin tissue, activate the skin's self-repair ability at the cellular level, and have an excellent repair effect on the skin barrier. It can repair the skin barrier from the root. It basically helps promote the production and secretion of collagen and elastic fibers, improves sensitive skin, and makes the skin firm and elastic; and the exosome active factors come from cells, can remain active for a long time, will not be quickly metabolized by the human body, have good repair ability and last for a long time; can accelerate cell metabolism, and improve the skin's own repair and regeneration ability; in addition, exosomes are also involved in regulating various metabolisms in the body, inhibiting the production of inflammatory cytokines, which can fundamentally improve inflammation and sensitive skin, safely and effectively enhance skin barrier function and repair barriers, improve skin tolerance, and gradually restore the skin to a healthy state. (2) Ceramide: Ceramide is the main component of lipids in the stratum corneum of the skin. It forms a network structure in the stratum corneum, which helps maintain the moisture of the skin, prevents water loss, and keeps the skin hydrated. The moisturizing effect of ceramide is 16 times that of hyaluronic acid. It can also promote the penetration of other nutrients. Ceramide forms a barrier in the gaps between keratinocytes of the skin by increasing the thickness of the stratum corneum of the epidermis and improving the skin's ability to retain water. It prevents the invasion of harmful external substances and resists external stimuli, thereby enhancing the skin's self-protection ability. At the same time, the signal regulation of ceramide and its metabolites can also regulate the inflammatory response in the skin, reduce the occurrence of skin allergies and inflammation, and thus protect the skin from damage. (3) Alpine edelweiss: Alpine edelweiss is a plant that grows in high altitude areas. In recent years, it has been widely used as an ingredient in skin care products due to its strong antioxidant and repair capabilities. Alpine edelweiss extract can promote the increase in the expression of AQP3 in the skin, increase the moisture content of the skin, enhance the skin's water retention capacity, and make the skin healthier and more moisturized; Alpine edelweiss extract can also effectively inhibit the release of pro-inflammatory factors such as IL-8, IL-6, and TNF-a, thereby reducing skin inflammation and enhancing the skin's barrier function, fundamentally solving the pseudo-problems of sensitive skin and damaged skin; at the same time, alpine edelweiss extract contains flavonoids and flavonoids, which have strong antioxidant effects, can effectively fight free radicals, inhibit the formation and deposition of melanin, and fade spots, thereby brightening the skin tone and making the skin whiter and more translucent.(4) Ecdoin: Ecdoin comes from halophilic bacteria in extreme environments. Ecdoin can bind to water molecules in the skin tissue, thereby increasing the number of water molecules and hydrophobic interactions, improving protein stability, helping the skin lock in moisture, avoiding water loss, repairing damaged cell barriers, and significantly reducing transepidermal water loss in patients with sensitive skin and atopic skin. Ecdoin can also promote keratin formation and help repair the skin barrier. It also shows good therapeutic effects on patients with mild to moderate atopic dermatitis. Ecdoin can also promote the growth of fibroblasts and the metabolism of epidermal cells, thereby promoting the rapid development of elastic fiber cells to enhance cell self-repair. It has the ability to reactivate aging and damaged cells, accelerate cell renewal and metabolism, increase the fluidity of cell membranes, and improve the adaptability of cells to extreme conditions; Ecdoin can also scavenge intracellular free radicals and inhibit lipid peroxidation, thereby protecting the skin from damage by environmental pollution and ultraviolet radiation. It combines with water molecules to form a stable protective complex, reducing the impact of external pollutants on cells; Ecdoin has a regulating effect on the skin microenvironment, can effectively inhibit the production of inflammatory mediators, reduce inflammatory responses, and achieve the effect of calming sensitive skin; the use of creams containing Ecdoin to treat patients with mild to moderate atopic dermatitis is effective. (5) Ergothioneine Matsutake Extract: Ergothioneine Matsutake Extract is a complex fermentation liquid extracted from Matsutake using a special strain and a special fermentation process. It contains ergothioneine, multiple amino acids and organic acids, trace mineral elements and other active substances. Ergothioneine neutralizes free radicals by sacrificing itself, thereby protecting cells from free radicals and oxygen ions. At the same time, ergothioneine can also protect and activate antioxidant components in the body, such as glutathionease and protease, and is a natural antioxidant. Many literatures have also shown that ergothioneine can participate in the protection of mitochondrial DNA and protect the length of telomerase, thereby achieving the effect of skin care and anti-aging. Ergothioneine also has the ability to inhibit the synthesis of melanin in cells, which helps reduce skin spots and pigmentation, thereby achieving the effect of whitening the skin. 1,2-Pentanediol, glycerin, hydrolyzed sodium hyaluronate, etc. can moisturize, enhance the skin's own defense capabilities, and improve the skin barrier function. Disodium EDTA can stabilize various active ingredients to prevent them from becoming ineffective due to interference from metal ions, thereby ensuring the effectiveness and safety of skin care products. Mannitol has certain antioxidant properties, which can prevent excessive keratinization and inflammatory reactions of the skin, and help reduce skin sensitivity, redness, and swelling caused by external stimuli. Trehalose has excellent cell protection properties and can help repair damaged skin barriers.

[0014] Figure 1 As shown, the testing and evaluation methods in the present invention are as follows: (1) Experimental subjects: Experimental group (the present invention): 30 cases, 15 males and 15 females, aged 16-42 years; Control group (placebo-ordinary repair fluid): 30 cases, 15 males and 15 females, aged 15-45 years old; The gender, age and other data of the two groups were statistically processed and showed no significant difference (P> 0.05), indicating that the two groups were comparable.

[0015] (2) Treatment method: The sensitive skin of the experimental group was wet-applied with the present invention on the whole face, and the sensitive skin of the control group was wet-applied with ordinary repair solution on the whole face. The skin repair time was recorded and the following evaluation was performed, and the occurrence of discomfort was recorded.

[0016] (3) Evaluation criteria: Evaluation is based on dry and peeling skin, facial redness and swelling, itching and pain.

[0017] Recovery: Symptoms and signs disappear by more than 95%.

[0018] Markedly effective: Clinical symptoms were alleviated by 80%-94%.

[0019] Improvement: Clinical symptoms are relieved by 50%-79%.

[0020] Ineffective: No improvement in clinical manifestations.

[0021] (4) Results: The results are shown in the following table.

[0022]

[0023] The difference analysis between the experimental group and the control group (P < 0.05) showed a significant difference, which was statistically significant. The effective rate of the experimental group was significantly higher than that of the control group, indicating that the experimental group could quickly repair sensitive skin, and the experimental group did not experience any discomfort, indicating that the invention had a good repair effect.

[0024] Figure 1 As shown, through the above technical scheme, the present invention combines NK cell exosomes, ceramide, alpine edelweiss, ectoine, ergothioneine matsutake extract, etc., which is suitable for skin sensitivity problems caused by various reasons. While fundamentally solving and quickly repairing sensitive skin, it replenishes nutrients for basal cells, soothes skin condition, and improves problems such as dry and rough skin and pigmentation.

[0025] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0026] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A dressing composition containing NK cell exosomes for repairing sensitive skin, characterized in that: Including NK cell exosomes, ceramide, alpine edelweiss, ectoine, ergothioneine agaric extract, 1,2-pentanediol, glycerol, hydrolyzed sodium hyaluronate, disodium EDTA, water, mannitol, and trehalose; the proportion of NK cell exosomes is 2%-4%, the proportion of ceramide is 4%-6%, the proportion of alpine edelweiss is 3.5%-6%, the proportion of ectoine is 2%-4%, the proportion of ergothioneine agaric extract is 2%-4%, the proportion of 1,2-pentanediol is 7%-9%, the proportion of glycerol is 2%-4%, the proportion of hydrolyzed sodium hyaluronate is 0.5%-1.5%, the proportion of disodium EDTA is 0.15%-0.4%, the proportion of water is 8%-70%, the proportion of mannitol is 5%-7%, and the proportion of trehalose is 0.5%-1.5% The preparation method of a NK cell exosome dressing composition for repairing sensitive skin comprises the following steps: S1: adding ceramide, alpine edelweiss, ectoine, ergothioneine matsutake extract, glycerin, hydrolyzed sodium hyaluronate, water, and NK cell exosomes into a beaker in proportion, placing the beaker in a water bath, and then stirring the raw materials in the beaker with an electric stirring device until they are dissolved and transparent to obtain a phase A finished product; S2: mixing mannitol and trehalose in proportion and dispensing them into 5 ml ampoules, and placing the ampoules in a freeze dryer to prepare freeze-dried powder to obtain a phase B finished product; S3: packaging the phase A finished product and the phase B finished product separately. During application, the phase A finished product and the phase B finished product are mixed in a ratio of 10-20:0.5-1.5, and then applied to the affected area to treat sensitive skin.

2. The dressing composition containing NK cell exosomes for repairing sensitive skin according to claim 1, characterized in that: The proportion of NK cell exosomes is 3%, the proportion of ceramide is 5%, the proportion of alpine edelweiss is 5%, the proportion of ectoine is 3%, the proportion of ergothioneine matsutake extract is 3%, the proportion of 1,2-pentanediol is 8%, the proportion of glycerin is 3%, the proportion of hydrolyzed sodium hyaluronate is 1%, the proportion of disodium EDTA is 0.3%, the proportion of water is 61.7%, the proportion of mannitol is 6%, and the proportion of trehalose is 1%.

3. The method for preparing the NK cell exosome dressing composition for repairing sensitive skin according to claim 1, characterized in that: The temperature of the water bath is 75-85°C, preferably 80°C.

4. The method for preparing the NK cell exosome dressing composition for repairing sensitive skin according to claim 1, characterized in that: The stirring rod of the electric stirring device rotates at a speed of 500-600 revolutions per minute, preferably 520 revolutions per minute.

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