Composition with immunity regulating effect

Abstract

The present invention discloses a composition with the effect of regulating immunity, and the composition is composed of the following components in parts by weight: 1 to 50 parts of Cordyceps militaris extract, 1 to 50 parts of Matsutake extract, 1 to 80 parts of Ganoderma lucidum extract, and 1 to 80 parts of bird's nest extract. Each component in the composition provided by the present invention is a natural extract, has no obvious side effects on the human body, can be metabolized normally by the human body, and each component complements and interacts with each other, can improve the body weight of cyclophosphamide-induced immunosuppressed mice, increase the spleen and thymus index of cyclophosphamide-induced immunosuppressed mice, increase the number of white blood cells and neutrophils in the blood of cyclophosphamide-induced immunosuppressed mice and the proportion of lymphocytes, thereby improving the immunity of immunosuppressed mice.

Description

Technical Field

[0001] The invention belongs to the field of medical and health care products, and in particular relates to a composition with the effect of regulating immunity. Background Art

[0002] Cordyceps militaris, also known as northern winter Cordyceps militaris or northern cordyceps, was first discovered in Jilin Province, China in 1958. Existing studies have shown that Cordyceps militaris has similar physical structure and effects to wild Cordyceps sinensis, but its efficacy is better. Cordyceps militaris contains rich chemical components and has health benefits such as anti-tumor and immune function regulation. Cordycepin (3′-deoxyadenosine, 3′-Deoxyadenosine), also known as cordycepin, is a nucleoside antibiotic with multiple biological activities extracted from the fermentation broth of Cordyceps militaris or Cordyceps sinensis. Modern pharmacological studies have shown that it has a wide range of physiological function regulation effects on the immune system, nervous system, motor system, endocrine system, etc. Foreign researchers used cell culture fluid containing cordycepin to culture human peripheral blood mononuclear cells in vitro and monitored the dynamic changes of their secretion of interleukin IL-10 and IL-2. The results showed that cordycepin can increase the secretion of IL-10 and promote the expression of IL-10mRNA. In addition, cordycepin can inhibit the production of IL-2 induced by phytohemagglutinin and the proliferation of peripheral blood mononuclear cells. Regulatory T cells (Treg) play an important role in inducing immune tolerance and immune regulation. Cordycepin can act on mature dendritic cells, induce the proliferation of Treg, and play an indirect role in the treatment of autoimmune diseases. In the study of the effect of cordycepin on delayed-type hypersensitivity reaction in mice and its immune mechanism, different doses of cordycepin solutions were set up as test groups by intraperitoneal administration, and compared with the negative control group of saline. The results showed that cordycepin can significantly inhibit the occurrence of contact dermatitis in mice, and is positively correlated with the dosage. At the same time, no obvious toxic effect on spleen tissue was observed. In addition, cordycepin can inhibit cell division, promote cell differentiation, change the distribution of material structure on the cell membrane, and promote the transformation of T lymphocytes. It can also improve the phagocytic function of the body's mononuclear macrophage system, activate macrophages to produce cytotoxins to directly kill cancer cells, and cordycepin can also inhibit protein kinase activity, resist the cleavage of sugar groups by nucleotidylase, and regulate humoral immunity.

[0003] Matsutake, known as the king of mushrooms, is a pure natural rare and precious edible and medicinal mushroom. Matsutake is mainly distributed in temperate and cold temperate regions above 3,500 meters above sea level in the three northeastern provinces of my country, Yunnan Province and Tibet Autonomous Region. It is mostly found in pine and oak forests and cannot be cultivated artificially at present. Matsutake has a strong aroma, a smooth and refreshing taste, and is extremely delicious. It not only has high nutritional value, but also has special medicinal value. It is a rare medicinal food tonic. Studies have shown that Matsutake has anti-tumor, anti-fatigue, anti-oxidation and immunity-enhancing effects.

[0004] Ganoderma lucidum is a precious large medicinal fungus. Its fruiting bodies and spores are both medicinal materials. It is a traditional Chinese medicinal material belonging to the kingdom Fungi, phylum Basidiomycetes, class Agaricus, order Polyporaceae, family Ganodermaceae. Pharmacological studies have shown that taking Ganoderma lucidum products can significantly improve the body's disease resistance and enhance immunity. Modern studies have shown that Ganoderma lucidum contains a variety of active substances such as polysaccharides, triterpenes, sterols, trace elements and fatty acids. Ganoderma lucidum has many biologically active functions, including anti-inflammatory, antioxidant, anti-cancer and immunostimulatory effects. Therefore, Ganoderma lucidum has been used to treat various chronic diseases such as liver disease, nephritis, hypertension, arthritis, bronchitis, asthma and cancer. Among them, Ganoderma lucidum polysaccharides and Ganoderma lucidum triterpenes are widely considered to be the main biologically active components in Ganoderma lucidum. Ganoderma lucidum polysaccharides are heteropolysaccharides mainly composed of glucose, galactose, rhamnose, arabinose, mannose and fucose, and mostly exist in the form of proteoglycans. They play an important role in many diseases, especially their characteristics as immunomodulators and anticancer agents. Ganoderma triterpenoids are a type of highly oxidized lanostane. Ganoderma acid can inhibit the release of cellular histamine and also has anti-tumor, antioxidant, lipid-lowering, anti-HIV and antibacterial effects.

[0005] Edible Bird's Nest (EBN), also known as bird's nest, bird's root or bird's vegetable, is a nest made by the swiftlet and other swiftlets of the same genus using saliva and down feathers. It is mainly produced in Southeast Asian countries and the South my country Sea Islands. The consumption of bird's nest has a long history in my country. Since it was introduced to my country, bird's nest has been regarded as a tonic. It is rich in nutrition and can nourish the kidneys and lungs, nourish the spleen and stomach, and regulate immunity.

[0006] The drugs for improving immunity in the prior art have serious toxic side effects and single effects. Therefore, it is of great significance to develop a product that has no obvious side effects on the body and regulates the body's immunity from different angles. Summary of the invention

[0007] The technical problem to be solved by the present invention is to provide a composition with the effect of regulating immunity, which has no obvious side effects on the body and regulates the body's immunity from different angles, in view of the shortcomings of the prior art.

[0008] In order to achieve the above object, the technical solution of the present invention is as follows:

[0009] A composition having the effect of regulating immunity, the composition is composed of the following components in parts by weight:

[0010] 1-50 parts of Cordyceps militaris extract;

[0011] 1-50 parts of Matsutake extract;

[0012] 1-80 parts of Ganoderma lucidum extract;

[0013] 1-80 parts of bird's nest extract.

[0014] Preferably, the composition consists of the following components in parts by weight:

[0015] 5-40 parts of Cordyceps militaris extract;

[0016] 5-40 parts of Matsutake extract;

[0017] 20-60 parts of Ganoderma lucidum extract;

[0018] 20-60 parts of bird's nest extract.

[0019] Preferably, the composition consists of the following components in parts by weight:

[0020] 10-30 parts of Cordyceps militaris extract;

[0021] 10-30 parts of Matsutake extract;

[0022] 30-50 parts of Ganoderma lucidum extract;

[0023] 30-50 parts of bird's nest extract.

[0024] Preferably, the composition consists of the following components in parts by weight:

[0025] 15-25 parts of Cordyceps militaris extract;

[0026] 15-25 parts of Matsutake extract;

[0027] 35-45 parts of Ganoderma lucidum extract;

[0028] 35-45 parts of bird's nest extract.

[0029] Further preferably, the composition consists of the following components in parts by weight:

[0030] 20 parts of Cordyceps militaris extract;

[0031] 20 parts of Matsutake extract;

[0032] 40 parts of Ganoderma lucidum extract;

[0033] 40 servings of bird's nest extract.

[0034] The preparation method of the Cordyceps militaris extract is as follows: evenly mixing Cordyceps militaris fruiting body dry powder with distilled water, extracting by hot reflux, collecting the extract, filtering, collecting the filtrate, concentrating, and freeze-drying to obtain the extract.

[0035] Preferably, the preparation method of the Cordyceps militaris extract is: evenly mix the Cordyceps militaris fruiting body dry powder and distilled water in a mass ratio of 1:10 to 20, place it in a boiling water bath for hot reflux extraction 3 times, each extraction time is 60 to 90 minutes, collect the extract, use a filter membrane (membrane pore size is not more than 0.001 micron) to filter, collect the filtrate, evaporate and concentrate the filtrate to 1 / 4 to 1 / 2 of the original volume; freeze-dry at -40 to -10°C for 60 to 90 minutes to obtain the extract.

[0036] Most preferably, the preparation method of the Cordyceps militaris extract is: placing the Cordyceps militaris fruiting body in a vacuum drying oven at 50-60° C. for 12-24 hours, then taking it out, crushing the sample, passing it through a 60-mesh sieve, and obtaining a Cordyceps militaris fruiting body dry powder, mixing the Cordyceps militaris fruiting body dry powder with distilled water in a mass ratio of 1:10, placing it in a boiling water bath for hot reflux extraction for 90 minutes, filtering with filter paper, collecting the first extract and the first filter residue, adding distilled water to the first filter residue (the amount of distilled water added is the same as the amount of distilled water added in the first extraction), mixing evenly, and placing it in a boiling water bath for hot reflux extraction. The mixture was extracted for 90 minutes, filtered with filter paper, and the second extract and the second filter residue were collected. Distilled water was added to the second filter residue (the amount of distilled water added was the same as that of the distilled water added in the first extraction), and then mixed evenly. The mixture was placed in a boiling water bath and refluxed for 90 minutes. The mixture was filtered with filter paper, and the third extract was collected. The first, second and third extracts were combined, and the mixture was filtered with a filter membrane (the pore size of the membrane was 0.001 micron). The filtrate was collected, and the filtrate was evaporated and concentrated to 1 / 3 of the original volume, freeze-dried at -40°C for 90 minutes, crushed, and passed through a 100-mesh sieve to obtain the Cordyceps militaris extract.

[0037] The preparation method of the Tricholoma matsutake extract is as follows: evenly mix the dry powder of Tricholoma matsutake fruiting bodies with distilled water, extract in a water bath, collect the extract, filter, collect the filtrate, concentrate, precipitate with anhydrous ethanol, collect the precipitate, and freeze-dry to obtain the extract.

[0038] Preferably, the preparation method of the Tricholoma matsutake extract is: evenly mix the dry powder of Tricholoma matsutake fruiting body and distilled water in a mass ratio of 1:10-20, extract in a water bath at 80-100°C for 3 times, each extraction time is 3-5 hours, collect the extract, filter with a diaphragm vacuum pump (membrane pore size is not more than 0.001 micron), collect the filtrate, evaporate and concentrate the filtrate to 1 / 4-1 / 2 of the original volume to obtain a concentrated solution, add 2-4 times the volume of anhydrous ethanol to the concentrated solution, let it stand at 0-5°C for 12-24 hours, centrifuge, collect the precipitate, and vacuum freeze-dry at -40--10°C for 60-90 minutes to obtain the concentrate.

[0039] Most preferably, the preparation method of the Tricholoma matsutake extract is: mix the dry powder of Tricholoma matsutake fruiting body with distilled water in a mass ratio of 1:15, extract in a water bath at 90°C for 4 hours, filter with filter paper to obtain a first extract and a first residue, add distilled water to the first residue (the amount of distilled water added is the same as the amount of distilled water added in the first extraction), mix evenly, extract in a water bath at 90°C for 4 hours, filter with filter paper to obtain a second extract and a second residue, add distilled water to the second residue (the amount of distilled water added is the same as the amount of distilled water added in the first extraction). The first, second and third extracts were combined, filtered with a diaphragm (membrane pore size of 0.001 micron) and a vacuum pump, and the filtrate was collected. The filtrate was evaporated and concentrated to 1 / 3 of the original volume by a rotary evaporator to obtain a concentrated solution. 3 times the volume of anhydrous ethanol was added to the concentrated solution, and the solution was allowed to stand for precipitation at 4°C for 24 hours, centrifuged at 4500 rpm for 15 minutes, and the precipitate was collected. It was vacuum freeze-dried at -40°C for 90 minutes to obtain the Tricholoma matsutake extract.

[0040] The preparation method of the ganoderma lucidum extract is as follows: evenly mixing the ganoderma lucidum fruiting body dry powder with distilled water, extracting in a water bath, collecting the extract, concentrating it, and freeze-drying it to obtain the extract.

[0041] Preferably, the preparation method of the Ganoderma lucidum extract is: evenly mix the Ganoderma lucidum fruiting body dry powder and distilled water in a mass ratio of 1:20 to 40, extract twice in a water bath at 60 to 90° C., each extraction time being 1 to 3 hours, collect the extract, concentrate the extract under reduced pressure to 1 / 4 to 1 / 3 of the original volume, and freeze-dry at -40 to -10° C. for 60 to 90 minutes to obtain the extract.

[0042] Most preferably, the preparation method of the Ganoderma lucidum extract is: evenly mix the Ganoderma lucidum fruiting body dry powder and distilled water in a mass ratio of 1:30, extract in a water bath at 90°C for 2h, filter with filter paper, collect the first filtrate and the first filter residue, add deionized water to the first filter residue (the amount of distilled water added is the same as the amount of distilled water added in the first extraction), mix evenly, extract in a water bath at 90°C for 2h, filter with filter paper, collect the second filtrate, combine the first filtrate and the second filtrate, concentrate under reduced pressure to 1 / 3 of the original volume, and freeze-dry at -40°C for 90min to obtain the Ganoderma lucidum extract.

[0043] The preparation method of the bird's nest extract is as follows: crush the bird's nest, mix it evenly with distilled water, centrifuge it, collect the supernatant, and freeze-dry it.

[0044] Preferably, the preparation method of the bird's nest extract is: shear and crush the bird's nest and mix it evenly with distilled water in a mass ratio of 1:10 to 20, place it in a centrifuge at 5000 to 10000 r / min, centrifuge for 5 to 10 minutes at 0 to 4°C, collect the supernatant, and freeze-dry the supernatant at -40 to -10°C for 60 to 90 minutes to obtain the bird's nest extract.

[0045] Most preferably, the preparation method of the bird's nest extract is: shear and crush the bird's nest and mix it evenly with distilled water in a mass ratio of 1:15, place it in a high-speed refrigerated centrifuge at 7500r / min, centrifuge for 10min at 4°C, collect the supernatant, and freeze-dry it at -40°C for 90min to obtain the bird's nest extract.

[0046] The use of the composition with the effect of regulating immunity in the preparation of a product with the effect of regulating immunity is also within the protection scope of the present invention; the product is a health product, a nutritional food or a medicine.

[0047] The Cordyceps militaris extract of the present invention can effectively promote the secretion of nitric oxide (NO), nitric oxide synthase (NOS), inducible NOS (iNOS) and cytokine TNF-α by intraperitoneal macrophages of mice under appropriate concentration conditions, thereby enhancing the function of macrophages; studies have found that the Cordyceps militaris extract can significantly enhance the immune adhesion regulatory function of red blood cells to their own lymphocytes in cyclophosphamide-induced immunodeficiency model mice, and further research results have found that the extract can have a certain protective effect on the damage of humoral immune function and changes in genetic material of mice caused by γ-ray irradiation. Other studies have found that the Cordyceps militaris extract can regulate the levels of two cytokines, interferon-γ (IFN-γ) and interleukin IL-4, improve the immune imbalance of lymphocyte Th1 / Th2 movement, promote the restoration of a balanced state, and have an immune improvement effect.

[0048] Matsutake extract can enhance the activity of immune cells such as lymphocytes and phagocytes, and by enhancing the function of the body's immune system, it can promote immune cells to secrete related cytokines such as interferon to enhance immunity. Studies have shown that Matsutake extract has an immunomodulatory effect on aging mice injected with D-galactose. At high doses, it can significantly improve the organ index of mice and can independently activate spleen lymphocytes, coordinate ConA to promote spleen cell proliferation, and promote mice to secrete cytokines such as TNF-α and interleukin IL-6 to regulate immune function.

[0049] Ganoderma lucidum extract has significant immunoenhancing and immune restoring effects, can promote the immune ability of immunodeficient model mice, and antagonize the immunosuppressive state of mice caused by glucocorticoids. Studies have shown that Ganoderma lucidum extract can promote the cellular and humoral immune functions of mice in a dose-dependent manner, and also has a promoting effect on the non-specific immune function of mice. Ganoderma lucidum extract can also inhibit the body's cellular and humoral immunity, which is also the immune basis for its use in the treatment of autoimmune diseases and allergic reaction-related diseases. Studies have found that Ganoderma lucidum extract has an inhibitory effect on hyperimmunity, can inhibit delayed skin allergic reactions mediated by cellular immunity in mice, and can also inhibit the proliferation reaction of spleen cells and antibody production levels in allergic reaction mice.

[0050] Studies have shown that bird's nest extract has a certain effect on improving the immune function of mice with lung yin deficiency. Bird's nest extract can significantly promote the secretion of IgE, IgA, IgM and IgG3 antibodies, increase the phagocytic percentage and phagocytic index of macrophages in immunocompromised mice, promote the proliferation of spleen lymphocytes, and significantly enhance the immune function of mice.

[0051] The main active components in the preparation of the present invention are composed of Cordyceps militaris extract, Matsutake extract, Ganoderma lucidum extract and bird's nest extract. These four extracts complement each other and interact with each other, and have the effect of synergistically regulating the body's immunity. They can effectively improve the body weight of cyclophosphamide-induced immunosuppressed mice, reduce the spleen and thymus index of cyclophosphamide-induced immunosuppressed mice, and increase the number of white blood cells and neutrophils and the proportion of lymphocytes in the blood of cyclophosphamide-induced immunosuppressed mice, thereby improving the immunity of immunosuppressed mice. Compared with the prior art, the efficacy of the preparation of the present invention in enhancing immunity is significantly better.

[0052] Beneficial effects:

[0053] 1. The main active ingredients in the preparation of the present invention are composed of Cordyceps militaris extract, Matsutake extract, Ganoderma lucidum extract and Bird's Nest extract. These four extracts complement each other and interact with each other, and have a synergistic effect of regulating immunity. They can effectively improve the body weight of cyclophosphamide-induced immunosuppressed mice, reduce the spleen and thymus index of cyclophosphamide-induced immunosuppressed mice, and increase the number of white blood cells and neutrophils in the blood of cyclophosphamide-induced immunosuppressed mice, thereby improving the immunity of immunosuppressed mice. Compared with the prior art, the efficacy of the preparation of the present invention in enhancing immunity is significantly better.

[0054] 2. Compared with traditional products with immune-regulating effects, the main active ingredients in the preparation of the present invention are all natural extracts, have no obvious side effects on the human body, can be metabolized normally by the human body, and can effectively solve the problem of large toxic and side effects of immune-regulating drugs in the prior art. DETAILED DESCRIPTION

[0055] The present invention is further described below based on the following examples. It should be understood that the following embodiments are only used to illustrate the present invention, but not to limit the present invention.

[0056] Specific techniques or conditions not specified in the examples were carried out according to the techniques or conditions described in the literature in the field or according to the product instructions. The reagents or instruments used without indicating the manufacturer were all conventional products that can be purchased through regular channels.

[0057] The preparation methods of the Cordyceps militaris extract, Tricholoma matsutake extract, Ganoderma lucidum extract and scutellaria baicalensis acid extract described in the following examples are as follows:

[0058] The preparation method of the Cordyceps militaris extract is as follows: placing the Cordyceps militaris fruiting body in a vacuum drying oven at 60°C for 24 hours, then taking it out, crushing the sample, passing it through a 60-mesh sieve, obtaining Cordyceps militaris fruiting body dry powder, weighing 500g of Cordyceps militaris fruiting body dry powder in a round-bottom flask, adding 10 times the mass of distilled water to mix evenly, placing it in a boiling water bath for hot reflux extraction for 90 minutes, filtering with filter paper, collecting the first extract and the first filter residue, adding distilled water (5L) to the first filter residue and mixing evenly, placing it in a boiling water bath The mixture was extracted under moderate heat and refluxed for 90 min, filtered with filter paper, the second extract and the second filter residue were collected, distilled water (5 L) was added to the second filter residue and mixed evenly, and the mixture was placed in a boiling water bath and refluxed for 90 min, filtered, the third extract was collected, the first, second and third extracts were combined, and the mixture was filtered through a filter membrane (with a membrane pore size of 0.001 μm), the filtrate was collected, evaporated and concentrated to one third of the original volume, freeze-dried at -40°C for 90 min, crushed, and passed through a 100-mesh sieve to obtain a Cordyceps militaris extract.

[0059] The preparation method of the Tricholoma matsutake extract is as follows: 500 g of Tricholoma matsutake fruiting body dry powder is evenly mixed with 7.5 L of distilled water, extracted at 90° C. for 4 hours, filtered with filter paper to obtain a first extract and a first residue, 7.5 L of distilled water is added to the first residue, extracted at 90° C. for 4 hours, filtered with filter paper to obtain a second extract and a second residue, 7.5 L of distilled water is added to the second residue, extracted at 90° C. for 4 hours, filtered with filter paper to obtain a third extract and a third residue, the first, second and third extracts are combined, filtered with a diaphragm (membrane pore size of 0.001 micron) and a vacuum pump, the filtrate is collected, the filtrate is evaporated and concentrated to 1 / 3 of the original volume with a rotary evaporator to obtain a concentrated solution, 3 times the volume of anhydrous ethanol is added to the concentrated solution, the solution is allowed to stand at 4° C. for 24 hours, centrifuged at 4500 rpm for 15 minutes, the precipitate is collected, and vacuum freeze-dried at -40° C. for 90 minutes to obtain the Tricholoma matsutake extract.

[0060] The preparation method of the Ganoderma lucidum extract is as follows: 500 g of Ganoderma lucidum fruiting body dry powder is evenly mixed with 15 L of deionized water, extracted in a water bath at 90° C. for 2 hours, filtered with filter paper, a first filtrate and a first filter residue are collected, 15 L of deionized water is added to the first filter residue, extracted in a water bath at 90° C. for 2 hours, filtered with filter paper, a second filtrate is collected, the first filtrate and the second filtrate are mixed, concentrated under reduced pressure to one third of the original volume, and freeze-dried at -40° C. for 90 minutes to obtain the Ganoderma lucidum extract.

[0061] The preparation method of the bird's nest extract is as follows: 500g of the bird's nest is sheared and crushed, mixed with 7.5L of distilled water, centrifuged in a high-speed refrigerated centrifuge at 7500r / min and 4°C for 10min, the supernatant is collected, and freeze-dried at -40°C for 90min to obtain the bird's nest extract.

[0062] The mice used in the following examples are male Balb / c mice.

[0063] Example 1 Preparation of Formulation 1

[0064] Weigh 20 mg of Cordyceps militaris extract, 20 mg of Matsutake extract, 40 mg of Ganoderma lucidum extract and 40 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir well to dissolve, thereby obtaining Preparation 1.

[0065] Example 2 Preparation of Formulation 2

[0066] Weigh 10 mg of Cordyceps militaris extract, 20 mg of Matsutake extract, 40 mg of Ganoderma lucidum extract and 40 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation 2.

[0067] Example 3 Preparation of Formulation 3

[0068] Weigh 20 mg of Cordyceps militaris extract, 10 mg of Matsutake extract, 40 mg of Ganoderma lucidum extract and 40 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Composition 3.

[0069] Example 4 Preparation of Formulation 4

[0070] Weigh 20 mg of Cordyceps militaris extract, 20 mg of Matsutake extract, 20 mg of Ganoderma lucidum extract and 40 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation 4.

[0071] Example 5 Preparation of Formulation 5

[0072] Weigh 20 mg of Cordyceps militaris extract, 20 mg of Matsutake extract, 40 mg of Ganoderma lucidum extract and 20 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation 5.

[0073] Comparative Example 1 Preparation of Preparation A

[0074] Weigh 20 mg of Cordyceps militaris extract, dissolve it in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation A.

[0075] Comparative Example 2 Preparation of Formulation B

[0076] Weigh 20 mg of Tricholoma matsutake extract, dissolve it in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation B.

[0077] Comparative Example 3 Preparation of Formulation C

[0078] Weigh 40 mg of Ganoderma lucidum extract, dissolve it in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation C.

[0079] Comparative Example 4 Preparation of Formulation D

[0080] Weigh 40 mg of bird's nest extract, dissolve it in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation D.

[0081] Comparative Example 5 Preparation of Formulation E

[0082] Weigh 20 mg of Cordyceps militaris extract and 20 mg of Matsutake extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation E.

[0083] Comparative Example 6 Preparation of Formulation F

[0084] Weigh 20 mg of Cordyceps militaris extract and 40 mg of Ganoderma lucidum extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation F.

[0085] Comparative Example 7 Preparation of Formulation G

[0086] Weigh 20 mg of Cordyceps militaris extract and 40 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve to obtain Preparation G.

[0087] Comparative Example 8 Preparation of Formulation H

[0088] Weigh 20 mg of Tricholoma matsutake extract and 40 mg of Ganoderma lucidum extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation H.

[0089] Comparative Example 9 Preparation of Formulation I

[0090] Weigh 20 mg of Matsutake extract and 40 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve to obtain Preparation I.

[0091] Comparative Example 10 Preparation of Formulation J

[0092] Weigh 20 mg of Ganoderma lucidum extract and 40 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation J.

[0093] Comparative Example 11 Preparation of Formulation K

[0094] Weigh 20 mg of Cordyceps militaris extract, 20 mg of Matsutake extract and 40 mg of Ganoderma lucidum extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation K.

[0095] Comparative Example 12 Preparation of Formulation L

[0096] Weigh 20 mg of Cordyceps militaris extract, 40 mg of Ganoderma lucidum extract and 40 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation L.

[0097] Comparative Example 13 Preparation of Formulation M

[0098] Weigh 40 mg of Ganoderma lucidum extract, 20 mg of Matsutake extract and 40 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve, thereby obtaining Preparation M.

[0099] Comparative Example 14 Preparation of Formulation N

[0100] Weigh 20 mg of Cordyceps militaris extract, 20 mg of Matsutake extract and 40 mg of bird's nest extract, dissolve them together in 10 mL of sterile water, and stir thoroughly to dissolve to obtain Preparation N.

[0101] Example 6 Effects of different preparations on mouse body weight, organ indexes and peripheral blood routine

[0102] (1) Experimental methods

[0103] a) Experimental animal grouping and drug administration

[0104] The mice used in the experiment were 6-8 week old male Balb / c mice, weighing (18±2) g, purchased from Nanjing Qinglongshan Animal Breeding Farm and raised in the Animal Center of Nanjing University of Technology. Before the experiment, the mice were placed in a sterile environment, with free access to food and water at 22-26°C and 45-55% humidity, and a 12 / 12 hour light / dark cycle. They were adaptively raised for 1 week to adapt to the environment before the experiment. The mice were randomly divided into 21 groups, 10 in each group, and the specific groupings were as follows: Group 1 was a blank control group, Group 2 was a pathological model group, Groups 3 to 7 were Example groups 1 to 5, and Groups 8 to 21 were Comparative Examples 1 to 14. The treatment method for each group of mice is as follows:

[0105] Blank control group (Contorl): 10 healthy mice, for the 1st to 11th day of the experiment, each mouse was intragastrically administered with 60 mg / kg normal saline every day; on the 12th, 13th and 14th day, normal saline was intraperitoneally injected at a dose of 60 mg / kg.

[0106] Pathological model group (Model): 10 healthy mice were gavaged with normal saline at a dose of 60 mg / kg per mouse every day from day 1 to 11 of the experiment; cyclophosphamide was intraperitoneally injected at a dose of 60 mg / kg on days 12, 13, and 14 to create a cyclophosphamide-induced immunosuppressed mouse model.

[0107] Groups 1 to 5 of Examples: The feeding environment and modeling method were the same as those of the pathological model group. There were 10 cyclophosphamide-induced immunosuppressed mice in each group. On days 1 to 14 of the experiment, each mouse was given the drug by gavage at a dose of 100 mg / kg per day. The drugs given to the mice in groups 1 to 5 of Examples were preparations 1 to 5 prepared in Examples 1 to 5, respectively.

[0108] Comparative Examples 1 to 14 groups: The feeding environment and modeling method were the same as those of the pathological model group, with 10 cyclophosphamide-induced immunosuppressed mice in each group. On days 1 to 14 of the experiment, each mouse was given the drug by gavage at a dose of 100 mg / kg per day. The drugs given to the mice in Comparative Examples 1 to 14 groups were preparations A to N prepared in Comparative Examples 1 to 14, respectively.

[0109] b) Weight changes

[0110] The weight of each mouse in each group was weighed and recorded before the experiment, and the weight of each mouse in each group was weighed and recorded after the experiment. The change in mouse weight = mouse weight after the experiment - mouse weight before the experiment.

[0111] c) Organ index

[0112] The thymus is a central immune organ and the site of differentiation and maturation of immune cells. The spleen is a peripheral immune organ where mature immune cells colonize and respond to immune responses. Changes in the thymus and spleen indexes can reflect the strength of the body's innate immune function. After the experiment, the mice were killed, the thymus and spleen were removed, washed with saline, dried with filter paper, and weighed to calculate the thymus index and spleen index.

[0113] Spleen index = spleen weight (mg) / body weight (g);

[0114] Thymus index = thymus weight (mg) / body weight (g).

[0115] d) Routine peripheral blood tests

[0116] The blood routine data can directly reflect the effect of the extract on cells in the peripheral blood of immunocompromised mice. After the experiment, the mice were anesthetized with 5% chloral hydrate (0.05 g / mL), and whole blood was collected in anticoagulant tubes through the superior and inferior vena cava using a blood collection needle. The number of white blood cells (WBC), neutrophils (NEUT) and lymphocytes (LYMPH) in the blood of mice were detected using an automatic cell analyzer.

[0117] (2) Effects of different preparations on mouse body weight and organ indexes

[0118] The results of the weight, spleen index and thymus index of mice in each experimental group are shown in Table 1. The results show that compared with the blank control group, the weight, spleen index and thymus index of mice in the pathological model group were significantly reduced, indicating that the immunosuppressive mouse model was successfully established; compared with the pathological model group, the weight, spleen index and thymus index of all drug administration groups were increased, among which the weight and organ index of the embodiment 1 group were significantly increased, and its effect was the best. The effect of enhancing the immunity of immunosuppressed mice in the embodiment 1 group was the best, followed by the embodiment 5 group, the embodiment 3 group, the embodiment 4 group and the embodiment 2 group; and the effects of the embodiments 1 to 5 groups were much better than those of the comparative examples 1 to 14 groups, especially the embodiment 1 group, indicating that the Cordyceps militaris extract, the pine mushroom extract, the ganoderma lucidum extract and the bird's nest extract in the composition of the present invention played a synergistic effect, and synergistically improved the weight, spleen index and thymus index of immunosuppressed mice, thereby improving the immunity of immunosuppressed mice.

[0119] Table 1 Body weight, spleen index and thymus index of mice in each experimental group

[0120]

[0121]

[0122] Note: 1. The data in the table are average values; 2. *** indicates a significance level of p<0.001 compared with the pathological model group, ** indicates a significance level of p<0.01 compared with the pathological model group, and * indicates a significance level of p<0.05 compared with the pathological model group.

[0123] (2) Effects of different preparations on peripheral blood routine in mice

[0124] The results of peripheral blood routine tests of mice in each experimental group are shown in Table 2. The results show that compared with the blank control group, the number of white blood cells (WBC), lymphocyte (LYMPH) ratio, and neutrophil (NEUT) in the peripheral blood of mice in the pathological model group are significantly reduced, indicating that the immunosuppressive mouse model is successfully modeled. Compared with the pathological model group, the number of white blood cells, lymphocyte ratio, and neutrophil number in the blood routine tests of immunosuppressive mice can be significantly increased in the embodiment 1 group, followed by the embodiment 5 group, the embodiment 3 group, the embodiment 4 group, and the embodiment 2 group; and the effect of the embodiment 1 to 5 groups is much better than that of the comparative example 1 to 14 groups, especially the embodiment 1 group, indicating that the Cordyceps militaris extract, the pine mushroom extract, the ganoderma lucidum extract, and the bird's nest extract in the composition of the present invention play a synergistic effect, and the number of white blood cells, lymphocytes, and neutrophils in the blood of immunosuppressive mice is synergistically increased, thereby improving the immunity of immunosuppressive mice.

[0125] Table 2 Peripheral blood routine data of mice in each experimental group

[0126]

[0127]

[0128] Note: 1. The data in the table are average values; 2. *** indicates a significance level of p<0.001 compared with the pathological model group, ** indicates a significance level of p<0.01 compared with the pathological model group, and * indicates a significance level of p<0.05 compared with the pathological model group.

[0129] The present invention provides a concept and method for a composition having the effect of regulating immunity. There are many methods and approaches to realize the technical solution. The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention. All components not specified in this embodiment can be realized by existing technologies.

Claims

1. A composition having the effect of regulating immunity, characterized in that: The composition is composed of the following components in parts by weight: 1-50 parts of Cordyceps militaris extract; 1-50 parts of Matsutake extract; 1-80 parts of Ganoderma lucidum extract; 1-80 parts of bird's nest extract.

2. The composition having the effect of regulating immunity according to claim 1, characterized in that: The composition is composed of the following components in parts by weight: 5-40 parts of Cordyceps militaris extract; 5-40 parts of Matsutake extract; 20-60 parts of Ganoderma lucidum extract; 20-60 parts of bird's nest extract.

3. The composition having the effect of regulating immunity according to claim 1, characterized in that: The composition is composed of the following components in parts by weight: 10-30 parts of Cordyceps militaris extract; 10-30 parts of Matsutake extract; 30-50 parts of Ganoderma lucidum extract; 30-50 parts of bird's nest extract.

4. The composition having the effect of regulating immunity according to claim 1, characterized in that: The composition is composed of the following components in parts by weight: 15-25 parts of Cordyceps militaris extract; 15-25 parts of Matsutake extract; 35-45 parts of Ganoderma lucidum extract; 35-45 parts of bird's nest extract.

5. The composition having the effect of regulating immunity according to claim 1, characterized in that: The composition is composed of the following components in parts by weight: 20 parts of Cordyceps militaris extract; 20 parts of Matsutake extract; 40 parts of Ganoderma lucidum extract; 40 servings of bird's nest extract.

6. The composition having the effect of regulating immunity according to any one of claims 1 to 5, characterized in that: The preparation method of the Cordyceps militaris extract comprises the following steps: evenly mixing Cordyceps militaris fruiting body dry powder with distilled water, performing heat reflux extraction, collecting the extract, filtering, collecting the filtrate, concentrating, and freeze-drying to obtain the extract.

7. The composition having the effect of regulating immunity according to any one of claims 1 to 5, characterized in that: The preparation method of the Tricholoma matsutake extract comprises the following steps: uniformly mixing dry powder of Tricholoma matsutake fruiting bodies with distilled water, extracting in a water bath, collecting the extract, filtering, collecting the filtrate, concentrating, using anhydrous ethanol for alcohol precipitation, collecting the precipitate, and freeze-drying to obtain the extract.

8. The composition having the effect of regulating immunity according to any one of claims 1 to 5, characterized in that: The preparation method of the ganoderma lucidum extract comprises the following steps: evenly mixing the ganoderma lucidum fruiting body dry powder with distilled water, extracting in a water bath, collecting the extract, concentrating it, and freeze-drying it to obtain the extract.

9. The composition having the effect of regulating immunity according to any one of claims 1 to 5, characterized in that: The preparation method of the bird's nest extract is as follows: crushing the bird's nest and mixing it evenly with distilled water, centrifuging, collecting the supernatant, and freeze-drying to obtain the bird's nest extract.

10. Use of the composition having the effect of regulating immunity according to any one of claims 1 to 5 in the preparation of a product having the effect of regulating immunity.

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