Chinese herbal medicine extracellular vesicle composition as well as preparation method and application thereof

Abstract

The invention relates to a Chinese herbal medicine extracellular vesicle composition and a preparation method and application thereof, and the Chinese herbal medicine extracellular vesicle composition comprises at least four of turmeric extracellular vesicles, polygonum cuspidatum extracellular vesicles, polygonum multiflorum extracellular vesicles, cacumen biotae extracellular vesicles and dried ginger extracellular vesicles. Wherein the Chinese herbal medicine extracellular vesicle composition contains turmeric extracellular vesicles. The Chinese herbal medicine extracellular vesicle composition provided by the invention has the effects of growing hair and preventing hair loss, is safe and free of toxic and side effects, and can be easily taken by cells.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to a Chinese herbal medicine extracellular vesicle composition, a preparation method and application thereof. Background Art

[0002] Currently, surgery and drugs are commonly used to treat hair loss. Surgery has the disadvantages of being time-consuming, expensive, and limited in the number of hair follicles. Drugs such as finasteride and minoxidil are limited by their long treatment cycles, limited efficacy, adverse reactions, and easy recurrence. Therefore, finding safe and effective natural drugs has become a hot spot and difficulty in the treatment of hair loss. Traditional Chinese medicine has been used in hair loss for a long time in my country, and its clinical efficacy is significant. At present, many natural medicinal plants and active ingredients with high safety and significant efficacy have been discovered, such as Polygonum multiflorum, dried ginger, black sesame, and Polygonum cuspidatum.

[0003] Extracellular vesicles (EVs) are nanoscale (30-150nm) membrane vesicles released by cells into the extracellular matrix. They are involved in cell communication, migration, angiogenesis, and tumor cell growth. They are widely present in various body fluids and cell supernatants, and stably carry important signal molecules. Plant EVs, especially Chinese herbal medicine (medicinal plant) EVs, are considered to be an important emerging field in recent years. Chinese herbal medicine vesicles are derived from natural plants and have higher safety. There are few specific applications of extracellular vesicle membrane structure vesicles derived from plant cells. Although the hair growth and anti-hair loss effects of extracts or single components of Polygonum cuspidatum, Polygonum multiflorum, Platycladus orientalis, dried ginger, and turmeric have been reported (such as patents CN117530901B, CN117180159A, CN110559418B, CN109674722B), there are no reports on the use of Chinese herbal medicines such as turmeric, Polygonum multiflorum, and dried ginger as a treatment for hair loss.

[0004] Studies have shown that curcumin isolated from turmeric can inhibit steroid 5-α reductase to achieve anti-androgen activity. The hair loss drugs finasteride and dutasteride mainly inhibit 5-α reductase to treat hair loss. In addition, curcumin has powerful antioxidant and anti-inflammatory effects, which can reduce scalp inflammation and improve hair follicle health. Some animal studies have shown that curcumin can improve hair growth.

[0005] In view of this, there is an urgent need for a Chinese herbal extracellular vesicle composition with good anti-hair loss and hair growth efficacy, high safety and few side effects. The combination formula is adopted, and the multi-dimensional combination of turmeric and a variety of Chinese herbal extracellular vesicles is used to promote hair follicle cell proliferation, promote hair follicle cell growth factor secretion, regulate hair follicle cell growth-related genes, remove hair follicle cell reactive oxygen ROS levels, and anti-inflammatory, etc., to achieve a multi-faceted and efficient hair growth and anti-hair loss effect. Summary of the invention

[0006] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and provide a Chinese herbal extracellular vesicle composition which has the efficacy of hair growth and hair loss prevention and is safe and has no toxic side effects.

[0007] To achieve the above object, the technical solution adopted by the present invention is: The present invention provides a Chinese herbal medicine extracellular vesicle composition, which comprises at least four of turmeric extracellular vesicles, polygonum cuspidatum extracellular vesicles, polygonum multiflorum extracellular vesicles, platycladus orientalis leaf extracellular vesicles and dried ginger extracellular vesicles, wherein the Chinese herbal medicine extracellular vesicle composition comprises turmeric extracellular vesicles.

[0008] Preferably, the Chinese herbal extracellular vesicle composition includes turmeric extracellular vesicles, polygonum cuspidatum extracellular vesicles, polygonum multiflorum extracellular vesicles, platycladus orientalis leaf extracellular vesicles and dried ginger extracellular vesicles.

[0009] Preferably, the mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is (1-10): (0.1-2): (0.1-2): (0.5-5): (0.1-2).

[0010] Preferably, the mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is (1-6): (0.5-2): (0.5-2): (0.5-3): (0.5-2).

[0011] Preferably, the mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is 4:1:1:2:1.

[0012] Preferably, the mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is 2:1:1:2:1.

[0013] Preferably, the mass ratio of the turmeric extracellular vesicles, the knotweed extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is 1:1:1:2:1.

[0014] The present invention also provides a method for preparing the Chinese herbal extracellular vesicle composition described in any of the above items, comprising taking turmeric, knotweed, polygonum multiflorum, Platycladus orientalis leaves or dried ginger respectively, soaking, breaking the wall, centrifuging, and taking the supernatant; ultracentrifuging the obtained supernatant, taking the precipitate and resuspending it with buffer, mixing the obtained extracellular vesicles, and obtaining the Chinese herbal extracellular vesicle composition.

[0015] The present invention also provides a composition with the efficacy of preventing hair loss and promoting hair growth, wherein the composition comprises any one of the above-mentioned Chinese herbal extracellular vesicle compositions or the Chinese herbal extracellular vesicle composition prepared by the above-mentioned method.

[0016] Preferably, the concentration of the Chinese herbal extracellular vesicle composition in the composition having the efficacy of preventing hair loss and promoting hair growth is 0.1-15 g / L.

[0017] Preferably, the composition having the effect of preventing hair loss and promoting hair growth further comprises an emulsifier at a concentration of 1 to 15 g / L, a moisturizer at a concentration of 1 to 5 g / L, and a thickener at a concentration of 1 to 5 g / L; The emulsifier is selected from one or more of glyceryl stearate, glyceryl stearate / PEG-stearate, cetearyl alcohol, cetearyl alcohol / PEG-20 stearate, alkyl glycosides having 8 to 16 alkyl carbon atoms, sodium isostearyl lactylate, etc.; The moisturizer is selected from one or more of glycerin, propylene glycol, sodium PCA, 1,3-propanediol, butylene glycol, hexylene glycol, 1,2-pentanediol, caprylyl glycol, sorbitol, ethylhexylglycerin, trehalose, betaine, sodium hyaluronate, saccharide isomers, urea, allantoin and panthenol; The thickener is selected from one or more of xanthan gum, taro gum, ammonium acryloyldimethyltaurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, carbomer and hydroxyethyl cellulose.

[0018] The present invention also provides the use of any of the above-mentioned Chinese herbal extracellular vesicle compositions, or the Chinese herbal extracellular vesicle composition prepared by the above-mentioned method, or any of the above-mentioned compositions with anti-hair loss and hair growth effects in the preparation of products with anti-hair loss and hair growth effects.

[0019] Preferably, the application is the application of the Chinese herbal extracellular vesicles in the preparation of a product for promoting the secretion and expression level of VEGF in dermal papilla cells.

[0020] Preferably, the application is the application of the Chinese herbal extracellular vesicles in the preparation of a product that inhibits the expression levels of inflammatory factors IL-6 and BMP4 in hair papilla cells.

[0021] Effects of the Invention The Chinese herbal extracellular vesicle composition provided by the present invention has a synergistic effect between the components, so that the Chinese herbal extracellular vesicle composition can effectively promote the proliferation of hair follicle cells, promote the secretion of hair follicle cell growth factors, regulate the expression of hair follicle cell growth-related genes, remove the level of hair follicle cell reactive oxygen ROS, and have antibacterial and anti-inflammatory effects, thereby achieving efficient hair growth and anti-hair loss effects. The Chinese herbal extracellular vesicle composition provided by the present invention is safe and effective, can be used for a long time, has good compatibility with most medicines or daily necessities raw materials, and has a simple process for adding it to medicines or daily necessities, and is suitable for industrial production. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Electron microscopic images of extracellular vesicle particles of turmeric, Polygonum cuspidatum, Polygonum multiflorum, Platycladus orientalis leaves, and dried ginger (red arrows indicate extracellular vesicles); Figure 2 The dermal papilla cells take up the Chinese herbal extracellular vesicle composition (red: PKH26-labeled Chinese herbal extracellular vesicle composition; blue: DAPI); Figure 3 The ex vivo skin of 8-week-old Bama miniature pigs was infiltrated with a Chinese herbal extracellular vesicle composition (white arrows indicate hair follicles, red arrows represent PKH26-labeled Chinese herbal extracellular vesicles, and blue arrows represent DAPI-stained cell nuclei); Figure 4 This is a bar graph showing that the extracellular vesicles prepared in Examples 1-2 promote the proliferation of hair papilla cells; Figure 5 This is a bar graph showing that the extracellular vesicles prepared in Examples 1 and 2 promote the secretion of VEGF, a growth factor of hair papilla cells; Figure 6 This is a bar graph showing that the extracellular vesicles prepared in Examples 1 and 2 inhibit the secretion of inflammatory factor IL-6 from hair papilla cells; Figure 7 The histogram of the effect of the extracellular vesicles prepared in Examples 1-2 on the expression of the hair growth-related gene BMP4 in dermal papilla cells; Figure 8 This is a bar graph showing the inhibition of ROS levels in dermal papilla cells by the extracellular vesicles prepared in Examples 1 and 2; Fig. 9 This is a test result diagram of the Chinese herbal extracellular vesicle anti-hair loss and hair growth essence on human body. DETAILED DESCRIPTION

[0023] In order to make the technical solutions and beneficial effects of the present invention more clearly understandable, the following is a detailed description by listing specific embodiments. The drawings are not necessarily drawn to scale, and local features may be enlarged or reduced to more clearly show the details of the local features; unless otherwise defined, the technical and scientific terms used herein have the same meanings as those in the technical field to which this application belongs.

[0024] A large number of studies have shown that extracellular vesicles produced by human cells (especially stem cells) can prevent and treat a variety of diseases. Studies have shown that stem cell extracellular vesicles can activate hair follicle dermal papilla cells and promote the transition of hair follicles from the resting phase to the growth phase, thereby starting the next growth phase and forming new hair shafts, allowing hair to regenerate. As the main mesenchymal component of the hair follicle unit, hair papilla cells play an important regulatory role in maintaining hair growth and inducing hair follicle regeneration, and extracellular vesicles can maintain the hair induction ability of hair papilla cells, thereby promoting hair regeneration. After extensive and in-depth research, the inventor unexpectedly discovered that a composition formed by extracellular vesicle particles derived from turmeric, Polygonum cuspidatum, Polygonum multiflorum, Platycladus orientalis, and dried ginger has the effect of preventing hair loss and promoting hair growth. More importantly, the inventor optimized the ratio of its components, and the obtained extracellular vesicle composition can promote hair follicle proliferation, promote the secretion of hair follicle cell growth factor, regulate the expression of hair follicle cell growth-related genes, remove the level of reactive oxygen ROS in hair follicle cells, and fight inflammation, so as to achieve a multi-faceted and efficient hair growth and hair loss prevention effect.

[0025] The present invention provides a Chinese herbal medicine extracellular vesicle composition, which includes at least four of turmeric extracellular vesicles, Polygonum cuspidatum extracellular vesicles, Polygonum multiflorum extracellular vesicles, Platycladus orientalis leaf extracellular vesicles and dried ginger extracellular vesicles, wherein the Chinese herbal medicine extracellular vesicle composition includes turmeric extracellular vesicles. The Chinese herbal medicine extracellular vesicle composition of the present invention uses pure Chinese medicinal plants as raw materials, and animal toxicology proves that it is safe, non-toxic and has no side effects. The diameter of each extracellular vesicle of the present invention is 20-500nm, which is a nano-scale raw material with good permeability, and is more easily absorbed and ingested by cells and skin to exert its efficacy.

[0026] In certain embodiments, the Chinese herbal extracellular vesicle composition includes turmeric extracellular vesicles, polygonum cuspidatum extracellular vesicles, polygonum multiflorum extracellular vesicles, and platycladus orientalis leaf extracellular vesicles.

[0027] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, and the platycladus orientalis leaf extracellular vesicles is (1-10):(0.1-2):(0.1-2):(0.5-5), for example, 1:1:1:1, 2:1:1:1, 3:1:1:1, 4:1:1:1, 5:1:1:1, 6:1:1:1, 7:1:1:1, 8:1:1:1, 9:1:1:1, 10:1:1:1, 1:2:1:1, 2:2:1:1, 3:2:1:1, 4:2:1:1, 5:2:1:1, 6:1:1:1, :2:1:1, 7:2:1:1, 8:2:1:1, 9:2:1:1, 10:2:1:1, 1:1:2:1, 2:1:2:1, 3:1:2:1, 4:1:2:1, 5:1:2:1, 6:1:2:1, 7:1:2:1, 8:1:2:1, 9:1:2:1, 10:1:2:1, 1:1:1:2, 2:1:1:2, 3:1:1:2, 4:1:1:2, 5:1:1:2, 6:1:1:2, 7:1:1:2, 8:1:1:2, 9:1:1:2, 10:1:1:2, etc.

[0028] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, and the platycladus orientalis extracellular vesicles is (1-6): (0.5-2): (0.5-3): (0.5-2).

[0029] In certain embodiments, the Chinese herbal extracellular vesicle composition includes turmeric extracellular vesicles, knotweed extracellular vesicles, polygonum multiflorum extracellular vesicles, and dried ginger extracellular vesicles.

[0030] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, and the dried ginger extracellular vesicles is (1-10):(0.1-2):(0.1-2):(0.1-2), for example, 1:1:1:1, 2:1:1:1, 3:1:1:1, 4:1:1:1, 5:1:1:1, 6:1:1:1, 7:1:1:1, 8:1:1:1, 9:1:1:1, 10:1:1:1, 1:2:1:1, 2:2:1:1, 3:2:1:1, 4:2:1:1, 5:2:1:1, 6:1:1:1, :2:1:1, 7:2:1:1, 8:2:1:1, 9:2:1:1, 10:2:1:1, 1:1:2:1, 2:1:2:1, 3:1:2:1, 4:1:2:1, 5:1:2:1, 6:1:2:1, 7:1:2:1, 8:1:2:1, 9:1:2:1, 10:1:2:1, 1:1:1:2, 2:1:1:2, 3:1:1:2, 4:1:1:2, 5:1:1:2, 6:1:1:2, 7:1:1:2, 8:1:1:2, 9:1:1:2, 10:1:1:2, etc.

[0031] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, the knotweed extracellular vesicles, the polygonum multiflorum extracellular vesicles, and the dried ginger extracellular vesicles is (1-6): (0.5-2): (0.5-2): (0.5-2).

[0032] In certain embodiments, the herbal extracellular vesicle composition includes turmeric extracellular vesicles, knotweed extracellular vesicles, platycladus orientalis leaf extracellular vesicles, and dried ginger extracellular vesicles.

[0033] In certain embodiments, the mass ratio of turmeric extracellular vesicles, polygonum cuspidatum extracellular vesicles, platycladus orientalis leaf extracellular vesicles, and dried ginger extracellular vesicles is (1-10): (0.1-2): (0.5-5): (0.1-2), for example, 1:1:1:1, 2:1:1:1, 3:1:1:1, 4:1:1:1, 5:1:1:1, 6:1:1:1, 7:1:1:1, 8:1:1:1, 9:1:1:1, 10:1:1:1, 1:2:1:1, 2:2:1:1, 3:2:1:1, 4:2:1:1, 5:2:1:1, 6:1:1:1, : 2:1:1, 7:2:1:1, 8:2:1:1, 9:2:1:1, 10:2:1:1, 1:1:2:1, 2:1:2:1, 3:1:2:1, 4:1:2:1, 5:1:2:1, 6:1:2:1, 7:1:2:1, 8:1:2:1, 9:1:2:1, 10:1:2:1, 1:1:1:2, 2:1:1:2, 3:1:1:2, 4:1:1:2, 5:1:1:2, 6:1:1:2, 7:1:1:2, 8:1:1:2, 9:1:1:2, 10:1:1:2, etc.

[0034] In certain embodiments, the mass ratio of turmeric extracellular vesicles, Polygonum cuspidatum extracellular vesicles, Platycladus orientalis leaf extracellular vesicles, and Zingiber officinale extracellular vesicles is (1-6): (0.5-2): (0.5-3): (0.5-2).

[0035] In certain embodiments, the Chinese herbal extracellular vesicle composition includes turmeric extracellular vesicles, polygonum multiflorum extracellular vesicles, platycladus orientalis leaf extracellular vesicles and dried ginger extracellular vesicles.

[0036] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is (1-10): (0.1-2): (0.5-5): (0.1-2), for example, 1:1:1:1, 2:1:1:1, 3:1:1:1, 4:1:1:1, 5:1:1:1, 6:1:1:1, 7:1:1:1, 8:1:1:1, 9:1:1:1, 10:1:1:1, 1:2:1:1, 2:2:1:1, 3:2:1:1, 4:2:1:1, 5:2:1:1, : 6:2:1:1, 7:2:1:1, 8:2:1:1, 9:2:1:1, 10:2:1:1, 1:1:2:1, 2:1:2:1, 3:1:2:1, 4:1:2:1, 5:1:2:1, 6:1:2:1, 7:1:2:1, 8:1:2:1, 9:1:2:1, 10:1:2:1, 1:1:1:2, 2:1:1:2, 3:1:1:2, 4:1:1:2, 5:1:1:2, 6:1:1:2, 7:1:1:2, 8:1:1:2, 9:1:1:2, 10:1:1:2, etc.

[0037] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis extracellular vesicles and the dried ginger extracellular vesicles is (1-6): (0.5-2): (0.5-3): (0.5-2).

[0038] In certain embodiments, the herbal extracellular vesicle composition includes turmeric extracellular vesicles, knotweed extracellular vesicles, polygonum multiflorum extracellular vesicles, platycladus orientalis leaf extracellular vesicles, and dried ginger extracellular vesicles.

[0039] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is (1-10):(0.1-2):(0.1-2):(0.5-5):(0.1-2), for example, 1:1:1:1:1, 2:1:1:1:1, 3:1:1:1:1, 4:1:1:1:1, 5:1:1:1:1, 6:1:1:1:1, 7:1:1:1:1, 8:1:1:1:1, 9:1:1:1:1, 10:1:1:1:1, 1:2:1:1:1, 2:2:1:1:1, 3:2:1:1:1, 4:2:1:1:1, 5:2:1:1:1, 6: 2:1:1:1, 7:2:1:1:1, 8:2:1:1:1, 9:2:1:1:1, 10:2:1:1:1, 1:1:2:1:1, 2:1:2:1:1, 3:1:2:1:1, 4:1:2:1:1, 5:1:2:1:1, 6:1:2:1:1, 7:1:2:1:1, 8:1:2:1 :1, 9:1:2:1:1, 10:1:2:1:1, 1:1:1:2:1, 2:1:1:2:1, 3:1:1:2:1, 4:1:1:2:1, 5:1:1:2:1, 6:1:1:2:1, 7:1:1:2:1, 8:1:1:2:1, 9:1:1:2:1, 10:1:1:2:1, etc.

[0040] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, polygonum cuspidatum extracellular vesicles, polygonum multiflorum extracellular vesicles, platycladus orientalis leaf extracellular vesicles and dried ginger extracellular vesicles is (1-6): (0.5-2): (0.5-2): (0.5-3): (0.5-2).

[0041] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is 4:1:1:2:1.

[0042] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, the knotweed extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis extracellular vesicles and the dried ginger extracellular vesicles is 2:1:1:2:1.

[0043] In certain embodiments, the mass ratio of the turmeric extracellular vesicles, the knotweed extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis extracellular vesicles and the dried ginger extracellular vesicles is 1:1:1:2:1.

[0044] The present invention also provides a method for preparing the Chinese herbal extracellular vesicle composition described in any of the above items, comprising taking turmeric, knotweed, polygonum multiflorum, Platycladus orientalis leaves or dried ginger respectively, soaking, breaking the wall, centrifuging, and taking the supernatant; ultracentrifuging the obtained supernatant, taking the precipitate and resuspending it with buffer, mixing the obtained extracellular vesicles, and obtaining the Chinese herbal extracellular vesicle composition.

[0045] The present invention also provides a composition with the efficacy of preventing hair loss and promoting hair growth, wherein the composition comprises the Chinese herbal extracellular vesicle composition described in any one of the above or the Chinese herbal extracellular vesicle composition prepared by the method described above.

[0046] In certain embodiments, the concentration of the Chinese herbal extracellular vesicle composition in the composition having the effect of preventing hair loss and promoting hair growth is 0.1-15 g / L, for example, 1 g / L, 1.5 g / L, 2 g / L, 2.5 g / L, 3 g / L, 3.5 g / L, 4 g / L, 4.5 g / L, 5 g / L, 5.5 g / L, 6 g / L, 6.5 g / L, 7 g / L, 7.5 g / L, 8 g / L, 8.5 g / L, 9 g / L, 9.5 g / L, 10 g / L, etc. Compared with surgical treatment, which has the disadvantages of long single treatment time, high price, and limited number of hair follicle units, the Chinese herbal extracellular vesicle composition of the present invention has low cost, can be used as a daily necessity, and is well compatible with other medicines or daily necessities.

[0047] In certain embodiments, the content of the Chinese herbal extracellular vesicle composition in the composition having the efficacy of preventing hair loss and promoting hair growth is 5-15 g / L.

[0048] In certain embodiments, the content of the Chinese herbal extracellular vesicle composition in the composition having the efficacy of preventing hair loss and promoting hair growth is 8-12 g / L.

[0049] In certain embodiments, the composition having the efficacy of preventing hair loss and promoting hair growth further comprises an emulsifier at a concentration of 1 to 15 g / L, a moisturizer at a concentration of 1 to 5 g / L, and a thickener at a concentration of 1 to 5 g / L; The emulsifier is selected from one or more of glyceryl stearate, glyceryl stearate / PEG, stearate, cetearyl alcohol, cetearyl alcohol / PEG-20 stearate, alkyl glycosides having 8 to 16 alkyl carbon atoms, sodium isostearyl lactylate, etc.; The moisturizer is selected from one or more of glycerin, propylene glycol, sodium PCA, 1,3-propanediol, butylene glycol, hexylene glycol, 1,2-pentanediol, caprylyl glycol, sorbitol, ethylhexylglycerin, trehalose, betaine, sodium hyaluronate, saccharide isomers, urea, allantoin and panthenol; The thickener is selected from one or more of xanthan gum, taro gum, ammonium acryloyldimethyltaurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, carbomer and hydroxyethyl cellulose.

[0050] In certain embodiments, the emulsifier is selected from alkyl glycosides having 8 to 16 carbon atoms in the alkyl group.

[0051] In a preferred embodiment, the emulsifier is selected from alkyl glycosides having 10 to 16 carbon atoms in the alkyl group.

[0052] In certain embodiments, the humectant is selected from glycerin.

[0053] In certain embodiments, the thickening agent is selected from carbomer.

[0054] In some embodiments, the concentration of the emulsifier is 5-15 g / L, for example, 5 g / L, 5.5 g / L, 6 g / L, 6.5 g / L, 7 g / L, 7.5 g / L, 8 g / L, 8.5 g / L, 9 g / L, 9.5 g / L, 10 g / L, etc.

[0055] In certain embodiments, the concentration of the moisturizer is 1-4 g / L, for example, 1 g / L, 1.5 g / L, 2 g / L, 2.5 g / L, 3 g / L, 3.5 g / L, 4 g / L, etc.

[0056] In certain embodiments, the concentration of the thickener is 1-4 g / L, for example, 1 g / L, 1.5 g / L, 2 g / L, 2.5 g / L, 3 g / L, 3.5 g / L, 4 g / L, etc.

[0057] The present invention also provides a method for preparing the composition with anti-hair loss and hair growth efficacy, the method comprising the following steps: mixing a thickener and water, stirring evenly, adding an emulsifier, heating to 75-85°C, stirring, adding a moisturizer to control the temperature to 75-85°C, stirring evenly, adding the Chinese herbal extracellular vesicle composition after homogenization, mixing evenly and discharging to obtain the composition with anti-hair loss and hair growth efficacy.

[0058] In certain embodiments, the homogenization temperature is controlled to be 75-85° C., and the homogenization time is controlled to be 20-40 min.

[0059] The present invention also provides the use of any of the above-mentioned Chinese herbal extracellular vesicle compositions, or the Chinese herbal extracellular vesicle composition prepared by the above-mentioned method, or any of the above-mentioned compositions with anti-hair loss and hair growth effects in the preparation of products with anti-hair loss and hair growth effects.

[0060] In certain embodiments, the application is the application of the Chinese herbal extracellular vesicles in the preparation of a product for promoting the secretion and expression level of VEGF in dermal papilla cells.

[0061] In certain embodiments, the application is the application of the Chinese herbal extracellular vesicles in the preparation of a product that inhibits the expression levels of inflammatory factors IL-6 and BMP4 in hair papilla cells.

[0062] The present invention is further described below by specific examples. Unless otherwise specified herein, "%" represents mass percentage. The materials and reagents in the following examples, unless otherwise specified, are all commonly used materials or reagents in the art, and can be obtained from commercial sources or synthesized by known methods. The experimental methods for which the conditions are not specified in the following implementation cases are usually carried out according to conventional experimental conditions or the conditions recommended by the manufacturers of the relevant reagents (kits). Example 1

[0063] Weigh 100g of turmeric, Polygonum cuspidatum, Polygonum multiflorum, Platycladus orientalis leaves, and dried ginger, wash them three times with ultrapure water to remove impurities, and place them in a clean beaker; add 500mL of PBS to each, soak overnight at 4℃; wash them three times with sterile water, transfer them to a wall-breaking machine, add 500mL of PBS, cover the lid tightly, and break the wall for 8-20min to ensure that there are no obvious block fragments; transfer the squeezed juice to a 50mL sterile centrifuge tube, centrifuge it at 500×g at 4℃ low speed centrifuge for 10min, discard the precipitate, and take the supernatant; centrifuge it at 2000×g at 4℃ low speed centrifuge for 20min min, discard the precipitate and take the supernatant; centrifuge at 4000×g at 4℃ low speed for 30min, discard the precipitate and take the supernatant; centrifuge at 10000×g at 4℃ high speed for 1h, discard the precipitate and take the supernatant; centrifuge at 110000×g at 4℃ ultracentrifuge for 70min, discard the supernatant, resuspend the precipitate with appropriate amount of PBS, and obtain extracellular vesicle particles of turmeric, knotweed, polygonum multiflorum, arborvitae leaf, and dried ginger (labeled as CL-Exo, PCM-Exo, PM-Exo, PLA-Exo, and ZO-Exo, respectively). Example 2

[0064] The extracellular vesicle particles of turmeric, Polygonum cuspidatum, Polygonum multiflorum, Platycladus orientalis leaves, and dried ginger from Example 1 were compounded according to different mass ratios to obtain different types of Chinese herbal extracellular vesicle compositions with anti-hair loss and hair growth effects (as shown in Table 1). If used for a short period of time, it can be directly stored in a -80°C refrigerator. For long-term use and transportation, a lyophilization protective agent can be added for freeze-drying.

[0065] Table 1 Example 3

[0066] The Chinese herbal extracellular vesicle composition derived from Example 2 was added to the essence matrix liquid to prepare the anti-hair loss and hair growth essence liquid. The specific operation was as follows: 100 mL of deionized water and 2 g of carbomer were added to a stirring pot and stirred for 3-10 min. After the thickener was completely dissolved, 10 g of an alkyl glycoside emulsifier with a carbon number of 14 was added and heated to 75-85° C.; after the emulsifier was basically dissolved, 3 g of glycerol was added and continued to stir for 5-10 min. The temperature was maintained at 75-85° C. After the material was completely dissolved, homogenized for 8-15 min, and continued to keep warm and stir for 20-40 min; 10 g of the Chinese herbal extracellular vesicle composition derived from Example 2 was added and stirred for 20-30 min, then PB was added to make the volume 1 L, the material was discharged, and various indicators were tested. HF-Mix1-8 corresponded to formulas 1-8 respectively.

[0067] PB formula: weigh 8 g NaCl, 0.2 g KCl, 1.44 g Na2HPO4 and 0.24 g KH2PO4, dissolve in 800 mL distilled water, adjust the pH value of the solution to 7.4 with HCl, and finally add distilled water to make up to 1 L.

[0068] Test Example 1 Electron microscopy results of extracellular vesicle particles The shapes of the extracellular vesicle particles of each Chinese herbal medicine prepared in Example 1 were analyzed by transmission electron microscopy (TEM).

[0069] According to the sample conditions, the extracellular vesicle particles from Example 1 were adjusted to a suitable concentration or viscosity. Use a pipette to draw about 15 μL of extracellular vesicles onto the copper mesh and let it stand for 1 min. Use filter paper to blot the extracellular vesicles on the copper mesh dry, and draw about 15 μL of 2% uranyl acetate staining solution to stain at room temperature for 1 min. If obvious adsorbents are visible on the copper mesh, pure water can be dripped onto the surface, quickly blotted off, and repeatedly washed several times. Use filter paper to blot the extracellular vesicles on the copper mesh dry. Observe and take pictures, and save the pictures. The results show ( Figure 1 ): Saucer-shaped vesicles can be observed in turmeric, Polygonum cuspidatum, Polygonum multiflorum, Platycladus orientalis leaves, and dried ginger.

[0070] Test Example 2: The situation of hair papilla cells taking up the Chinese herbal extracellular vesicle composition with the effect of preventing hair loss and promoting hair growth The Chinese herbal extracellular vesicle composition obtained from Example 2 was labeled with PKH26 to track the uptake of Chinese herbal extracellular vesicles by dermal papilla cells.

[0071] Dermal papilla cells were added to a 3.5 cm confocal dish at 200,000 cells / well and cultured for 24 hours. Take 1 mg of the Chinese herbal extracellular vesicle composition and dilute the sample to 1 mL with diluent C in the kit (Sigma, PKH26 red fluorescent cell labeling kit, MINI26-1KT). Take 6 μL of PKH26 dye (Sigma's MINI26-1KT) in the kit and add it to a test tube containing 1 mL of diluent C. Use a pipette to gently blow and mix continuously for 30 seconds. Let stand at room temperature for 5 minutes. Add 2 mL of PBS containing 10% BSA for quenching. Use serum-free medium to make the volume 30 mL. Centrifuge at 110,000g for 2 hours at 2-8°C. Use a pipette to gently blow and resuspend the extracellular vesicle particle pellet in 1 mL of serum-free medium for use.

[0072] The Chinese herbal extracellular vesicle composition labeled with PKH26 was added to the dermal papilla cells at 50 μg / mL and incubated for 12 hours. After the incubation, the cells were fixed with 4% paraformaldehyde for 10 minutes at room temperature, washed with PBS, stained with phalloidin for 30 minutes, and then DAPI was added to stain the cell nucleus, and then observed and photographed with a confocal microscope. The results showed ( Figure 2 ): The Chinese herbal extracellular vesicle composition can be taken up into the cells by hair papilla cells.

[0073] Test Example 3: Skin penetration of Chinese herbal extracellular vesicle composition Cut the back or abdominal skin of 8-week-old Bama miniature pigs into 1cm×1cm pieces for later use. Take 1 mg of the Chinese herbal extracellular vesicle composition HF-Mix2 prepared in Example 2, and dilute the sample to 1 mL with diluent C in the kit (Sigma, PKH26 Red Fluorescent Cell Labeling Kit MINI26-1KT). Take 6 μL of PKH26 dye in the kit and add it to a test tube containing 1 mL of diluent C. Use a pipette to blow gently and mix continuously for 30 seconds. Let stand at room temperature for 5 minutes. Add 2 mL of PBS containing 10% BSA for quenching. Use serum-free medium to make the volume fixed to 30 mL. Centrifuge at 110,000 g for 2 hours at 2-8°C. Use a pipette to blow gently and resuspend the extracellular vesicle pellet in 1 mL of serum-free medium for later use.

[0074] 1cm×1cm 8-week-old Bama miniature pig skin was placed in a 32°C environment, and 50μL of 1mg / mL PKH26-labeled Chinese herbal extracellular vesicle composition was dripped onto 1cm×1cm 8-week-old Bama miniature pig skin, evenly covering the entire epidermis, and incubated for 2h and 6h. After the incubation, cryosections were performed with a thickness of 10μm. After DAPI staining of the cell nucleus, the cells were observed and photographed under a fluorescence microscope. The results showed ( Figure 3): The Chinese herbal extracellular vesicle composition of the present invention can quickly penetrate into the skin of 8-week-old Bama miniature pigs within 2 hours and accumulate in large quantities in the hair follicles (white arrows).

[0075] Test Example 4: Chinese herbal extracellular vesicle composition can promote the proliferation of dermal papilla cells Dermal papilla cells were seeded into 96-well plates at a density of 5000 cells / 100 μL / well. Cells were cultured for 24 h. The cell supernatant was discarded, and 100 μL of negative control reagent (NC: T4 medium, Beso Biological, Cat. No. 04304P05), positive control reagent (PC: T4 medium + 10% fetal bovine serum), and experimental group reagent (extracellular vesicle particles from different sources of HF-Mix1 and HF-Mix2 in Example 1 and Example 2 (diluted with T4 medium to a protein concentration of 0.5 mg / mL)) were added, and cultured for 24 h. After discarding the supernatant, 100 μL of basal medium containing 10% CCK8 was added, incubated at 37°C for 2-4 h, and detected by microplate reader.

[0076] The results show that ( Figure 4 ): Extracellular vesicle particles derived from turmeric (CL-Exo), Platycladus orientalis leaves (PLA-Exo), and Polygonum cuspidatum (PCM-Exo) can significantly promote the proliferation of hair papilla cells. The Chinese herbal extracellular vesicle composition HF-Mix2 of the present invention can significantly promote the proliferation of hair papilla cells, and its effect is better than HF-Mix1 and each single turmeric, Platycladus orientalis leaves, Polygonum cuspidatum extracellular vesicle particles.

[0077] Test Example 5: Chinese herbal extracellular vesicle composition promotes growth factor secretion in androgenic alopecia model Dermal papilla cells were inoculated into 96-well plates at a density of 5000 cells / 100 μL / well. Cells were cultured for 24 h. The cell supernatant was discarded, and 100 μL of culture medium containing 1 μM dihydrotestosterone (DHT) was added to each well and cultured for 24 h to establish a cell androgenic alopecia model. PBS was discarded, and 100 μL of negative control reagent (NC: T4 culture medium), positive control reagent (PC: 1 μM finasteride, diluted with T4 culture medium), experimental group reagent (extracellular vesicle particles from different sources of HF-Mix1 and HF-Mix2 in Example 1 and Example 2 (diluted with T4 culture medium to a protein concentration of 0.5 mg / mL)) were added respectively, and the basal culture medium (BC) was added to the group not treated with DHT and cultured for 24 h. The supernatant was taken and the secretion of growth factor VEGF was detected by Jianglai Biological Vascular Endothelial Growth Factor (VEGF) Elisa detection kit.

[0078] The results show that ( Figure 5): In the cellular androgenic alopecia model, the extracellular vesicle particles derived from dried ginger (ZO-Exo), turmeric (CL-Exo), Platycladus orientalis (PLA-Exo), and Polygonum cuspidatum (PCM-Exo) can significantly promote the secretion of VEGF, a growth factor of hair papilla cells. The Chinese herbal extracellular vesicle composition HF-Mix2 of the present invention can also significantly promote the secretion of VEGF, a growth factor of hair papilla cells, and the effect is better than HF-Mix1 and each single dried ginger, turmeric, Platycladus orientalis, and Polygonum cuspidatum extracellular vesicle particles.

[0079] Test Example 6: Chinese herbal extracellular vesicle composition inhibits the secretion of inflammatory factors in androgenic alopecia model Dermal papilla cells were seeded into 96-well plates at a density of 5000 cells / 100 μL / well. The cells were cultured for 24 h. The cell supernatant was discarded, and 100 μL of culture medium containing 1 μM dihydrotestosterone (DHT) was added to each well and cultured for 24 h to establish a cell androgenic alopecia model. PBS was discarded, and 100 μL of negative control reagent (NC: T4 culture medium), positive control reagent (PC: 1 μM finasteride, diluted with T4 culture medium), experimental group reagent (extracellular vesicle particles from different sources of HF-Mix1 and HF-Mix2 in Example 1 and Example 2 (diluted with T4 culture medium to a protein concentration of 0.5 mg / mL)) were added respectively, and the basal culture medium (BC) was added to the group not treated with DHT and cultured for 24 h. The supernatant was taken and the secretion of the pro-inflammatory factor IL-6 was detected by the Lianke Bio-Inflammatory Factor Interleukin-6 (IL-6) Elisa Detection Kit.

[0080] The results show that ( Figure 6 ): In the cellular androgenic alopecia model, the extracellular vesicle particles derived from Polygonum multiflorum (PM-Exo) can significantly inhibit the secretion of inflammatory factor IL-6 from hair papilla cells. Although the Chinese herbal extracellular vesicle compositions HF-Mix1 and HF-Mix2 of the present invention significantly inhibit the secretion of inflammatory factor IL-6 from hair papilla cells, the effect of HF-Mix2 is better than that of HF-Mix1, turmeric, and Polygonum multiflorum extracellular vesicle particles.

[0081] Test Example 7: Effect of Chinese herbal extracellular vesicle composition on the expression of hair growth-related genes in androgenic alopecia model Dermal papilla cells were seeded into 24-well plates at a density of 20,000 cells / 500 μL / well. The cells were cultured for 24 hours. The cell supernatant was discarded, and 500 μL of culture medium containing 1 μM dihydrotestosterone (DHT) was added to each well and cultured for 24 hours to establish a cell androgenic alopecia model. The supernatant was discarded, and 500 μL of negative control reagent (NC: T4 culture medium), positive control reagent (PC: 1 μM finasteride, diluted with T4 culture medium), experimental group reagent (extracellular vesicle particles from different sources of HF-Mix1 and HF-Mix2 in Example 1 and Example 2 (diluted with T4 culture medium to a protein concentration of 0.5 mg / mL)) were added respectively, and the basal culture medium (BC) was added to the group not treated with DHT and cultured for 24 hours. The supernatant was discarded, the cells were collected, the RNA of the cells was extracted, and the expression of the hair growth-related gene BMP4 was detected.

[0082] The results showed that in the cellular androgenic alopecia model, Polygonum multiflorum (PM-Exo) and Curcuma longa (CL-Exo) could significantly inhibit the expression of BMP4, a gene negatively associated with hair growth in dermal papilla cells ( Figure 7 The Chinese herbal extracellular vesicle composition HF-Mix2 of the present invention can significantly inhibit the expression of BMP4, a gene negatively related to hair growth, in hair papilla cells ( Figure 7 ), and HF-Mix2 was better than single extracellular vesicles, and also better than HF-Mix1.

[0083] Test Example 8: Effect of Chinese herbal extracellular vesicle composition on the level of reactive oxygen species in oxidative stress hair loss model Dermal papilla cells were seeded into 24-well plates at a density of 20,000 cells / 500 μL / well. The cells were cultured for 24 hours. The supernatant was discarded, and 100 μL of negative control reagent (NC: T4 culture medium), positive control reagent (PC: 1 μM finasteride, diluted with T4 culture medium), and experimental group reagent (extracellular vesicle particles from different sources of HF-Mix1 and HF-Mix2 in Example 1 and Example 2 (diluted with T4 culture medium to a protein concentration of 0.5 mg / mL)) were added respectively, and culture was continued for 24 hours. 250 μM hydrogen peroxide (H2O2) culture medium was added to each well and cultured for 30 minutes to establish a cell oxidative stress hair loss model. The DCFH-DA fluorescent probe was used to determine the effects of different samples on the reactive oxygen levels of dermal papilla cells.

[0084] The results show that ( Figure 8): In the cellular oxidative stress hair loss model, the extracellular vesicle particles derived from dried ginger (ZO-Exo) and Polygonum cuspidatum (PCM-Exo) can significantly inhibit the level of reactive oxygen species (ROS) in hair papilla cells. The Chinese herbal extracellular vesicle composition HF-Mix2 of the present invention significantly inhibits the level of ROS in hair papilla cells, and the effect of HF-Mix2 is better than that of HF-Mix1, turmeric, dried ginger, and Polygonum cuspidatum extracellular vesicle particles.

[0085] Test Example 9: Chinese herbal extracellular vesicle composition promotes hair growth in isolated hair follicles of mice The back hair of 10-week-old C57BL / 6 mice was shaved, the skin was removed and washed with PBS 3-5 times, and the tissue blocks containing 3-4 hair follicles were peeled off under a microscope and placed in 24-well plates. 500 μL of negative control reagent (NC: T4 culture medium) and experimental group reagent (extracellular vesicle particles from different sources in Example 1 and Example 2 (diluted with T4 culture medium to a protein concentration of 0.5 mg / mL)) were added respectively, and cultured for 5 days, with the medium changed every two days, and the hair growth on the 2nd and 5th days was recorded respectively (N=10).

[0086] The results are shown in Table 2: Extracellular vesicle particles derived from Polygonum multiflorum (PM-Exo), Curcuma longa (CL-Exo), Platycladus orientalis (PLA-Exo), Polygonum cuspidatum (PCM-Exo), and Chinese herbal extracellular vesicle combination can promote hair growth of isolated hair follicles, among which HF-Mix2, HF-Mix7 and HF-Mix8 can significantly promote hair growth of isolated hair follicles, especially HF-Mix2 has the best effect.

[0087] Table 2

[0088] Test Example 10 Test on the effect of Chinese herbal extracellular vesicle composition on human hair loss prevention and growth 270 participants aged 30-50 years who had hair loss problems and had not used any hair loss treatment drugs recently were enrolled and randomly divided into 9 groups. The experimental group applied the Chinese herbal extracellular vesicle anti-hair loss and hair growth essence from Example 3 once a day in the morning and evening, and the control group applied the essence matrix (i.e., the essence without the extracellular vesicle composition) once a day in the morning and evening. The hair growth was tracked by taking photos every month, and the effective rate was calculated as: ((number of people with significant improvement*100%+number of people with slight improvement*50%) / 30)*100%. The test results of the Chinese herbal extracellular vesicle composition for anti-hair loss and hair growth in humans are shown in Table 3.

[0089] Table 3

[0090] After using the Chinese herbal extracellular vesicle composition for 3 months, hair growth was observed. More hair grew in the hair loss area on the top of the head, and the hair loss area shrank. Those with severe hair loss grew a lot of hair, most of which were fuzzy hair. In addition, the hairline of women became significantly narrower (such as Fig. 9 ).

[0091] It should be understood that the above embodiments are exemplary and are not intended to include all possible implementations included in the claims. Various modifications and changes may be made on the basis of the above embodiments without departing from the scope of the present disclosure. Similarly, the various technical features of the above embodiments may be arbitrarily combined to form other embodiments of the present invention that may not be explicitly described. Therefore, the above embodiments only express several implementations of the present invention and do not limit the scope of protection of the patent of the present invention.

Claims

1. A Chinese herbal extracellular vesicle composition, characterized in that: The Chinese herbal extracellular vesicle composition includes at least four of turmeric extracellular vesicles, polygonum cuspidatum extracellular vesicles, polygonum multiflorum extracellular vesicles, platycladus orientalis leaf extracellular vesicles and dried ginger extracellular vesicles, wherein the Chinese herbal extracellular vesicle composition contains turmeric extracellular vesicles.

2. The Chinese herbal extracellular vesicle composition according to claim 1, characterized in that: The Chinese herbal medicine extracellular vesicle composition comprises extracellular vesicles of turmeric, extracellular vesicles of polygonum cuspidatum, extracellular vesicles of polygonum multiflorum, extracellular vesicles of platycladus orientalis leaves and extracellular vesicles of dried ginger.

3. The Chinese herbal extracellular vesicle composition according to claim 2, characterized in that: The mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is (1-10): (0.1-2): (0.1-2): (0.5-5): (0.1-2).

4. The Chinese herbal extracellular vesicle composition according to claim 3, characterized in that: The mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is (1-6): (0.5-2): (0.5-2): (0.5-3): (0.5-2).

5. The Chinese herbal extracellular vesicle composition according to claim 4, characterized in that: The mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is 4:1:1:2:1; or, The mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is 2:1:1:2:1; or, The mass ratio of the turmeric extracellular vesicles, the polygonum cuspidatum extracellular vesicles, the polygonum multiflorum extracellular vesicles, the platycladus orientalis leaf extracellular vesicles and the dried ginger extracellular vesicles is 1:1:1:2:

1.

6. A method for preparing the Chinese herbal extracellular vesicle composition according to any one of claims 1 to 5, characterized in that: Take turmeric, Polygonum cuspidatum, Polygonum multiflorum, Platycladus orientalis leaves or dried ginger respectively, soak, break the wall, centrifuge, and take the supernatant; ultracentrifuge the obtained supernatant, take the precipitate and resuspend it with buffer, mix the obtained extracellular vesicles, and obtain the Chinese herbal extracellular vesicle composition.

7. A composition having the effect of preventing hair loss and promoting hair growth, characterized in that: The composition comprises the Chinese herbal extracellular vesicle composition according to any one of claims 1 to 5 or the Chinese herbal extracellular vesicle composition prepared by the method according to claim 6.

8. The composition having the effect of preventing hair loss and promoting hair growth according to claim 7, characterized in that: The concentration of the Chinese herbal extracellular vesicle composition in the composition with the efficacy of preventing hair loss and promoting hair growth is 0.1-15 g / L.

9. The composition having the effect of preventing hair loss and promoting hair growth according to claim 7 or 8, characterized in that: The composition with the effect of preventing hair loss and promoting hair growth further comprises an emulsifier at a concentration of 1 to 15 g / L, a moisturizer at a concentration of 1 to 5 g / L, and a thickener at a concentration of 1 to 5 g / L; The emulsifier is selected from one or more of glyceryl stearate, glyceryl stearate / PEG-stearate, cetearyl alcohol, cetearyl alcohol / PEG-20 stearate, alkyl glycosides having 8 to 16 alkyl carbon atoms, sodium isostearyl lactylate, etc.; The moisturizer is selected from one or more of glycerin, propylene glycol, sodium PCA, 1,3-propanediol, butylene glycol, hexylene glycol, 1,2-pentanediol, caprylyl glycol, sorbitol, ethylhexylglycerin, trehalose, betaine, sodium hyaluronate, saccharide isomers, urea, allantoin and panthenol; The thickener is selected from one or more of xanthan gum, taro gum, ammonium acryloyldimethyltaurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, carbomer and hydroxyethyl cellulose.

10. Use of the Chinese herbal extracellular vesicle composition according to any one of claims 1 to 5, or the Chinese herbal extracellular vesicle composition prepared by the method according to claim 6, or the composition with anti-hair loss and hair growth efficacy according to any one of claims 7 to 9 in the preparation of a product with anti-hair loss and hair growth efficacy.

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