Preparation method, device, equipment, medium and program product of plasma matrix

Abstract

The present application relates to a method, device, equipment, medium and program product for preparing a plasma matrix, and in particular to the field of plasma matrix preparation technology. The method is applied to a plasma matrix preparation platform, which includes an optical detection probe for real-time detection of the absorbance of the plasma matrix, including: performing preliminary centrifugation on multiple tubes of whole blood samples according to initial preparation parameters to obtain multiple tubes of primary plasma matrix; adjusting the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe; wherein there is a linear relationship between the absorbance and the preparation parameters; centrifuging the primary plasma matrix according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, so as to obtain a solid plasma matrix and a liquid plasma matrix. The embodiment of the present application can ensure the formation of the solid plasma matrix, and obtain two forms of plasma matrix products at the same time after the centrifugation, which shortens the centrifugation time to a certain extent.

Description

Technical Field

[0001] The present application relates to the field of terminal technology, and in particular to a method, device, equipment, medium and program product for preparing a plasma matrix. Background Art

[0002] Plasma matrix usually refers to the liquid components of plasma other than blood cells. It is a complex biological fluid that contains a variety of proteins, electrolytes, nutrients, metabolic wastes and various bioactive molecules, etc. It plays an important role in maintaining the normal physiological functions and internal environment stability of the human body. Plasma matrix can be used to promote postoperative wound healing, relieve postoperative pain, osteoarthritis injection treatment, facial beauty, soft tissue regeneration, bone regeneration, etc.

[0003] Since the plasma matrix was first introduced, it has undergone three major modifications. The fourth-generation plasma matrix, which is currently the most widely used, has shown unique advantages in terms of the quantity and preservation of internal bioactive components. The fourth-generation plasma matrix is ​​currently divided into two types: liquid plasma matrix and solid plasma matrix. During clinical treatment, some patients have a demand for both forms of products. Especially for solid plasma matrix, individual differences among patients often lead to insufficient platelet activation, weak fiber network formation, and even inability to coagulate in clinical practice.

[0004] The traditional solution to this situation is to extend the centrifugation time, but the extension of the centrifugation time will also bring many problems. First, too long centrifugation will promote the coagulation of liquid plasma matrix, resulting in failure to obtain the two products and difficulty in forming plasma matrix bone blocks in the later stage; secondly, extending the centrifugation time will increase the waiting time for patients and doctors; in addition, the preparation of traditional plasma matrix is ​​affected by the dual factors of centrifugal force and time. Only regulating the time will lead to uneven distribution of cells inside the plasma matrix, affecting the final enrichment effect. Summary of the invention

[0005] In order to solve the above technical problems or at least partially solve the above technical problems, the present application provides a method, device, equipment, medium and program product for preparing a plasma matrix, which can analyze the coagulation state of the plasma matrix in real time and prepare effective solid plasma matrix and liquid plasma matrix in the shortest time.

[0006] In order to achieve the above purpose, the technical solutions provided by the embodiments of the present application are as follows:

[0007] In a first aspect, the present application provides a method for preparing a plasma matrix, comprising: applying to a plasma matrix preparation platform, the plasma matrix preparation platform comprising an optical detection probe for real-time detection of the absorbance of the plasma matrix, the method comprising: performing preliminary centrifugation on multiple tubes of whole blood samples according to initial preparation parameters to obtain multiple tubes of primary plasma matrix; adjusting the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe; wherein there is a linear relationship between the absorbance and the preparation parameters; centrifuging the primary plasma matrix according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, so as to obtain a solid plasma matrix and a liquid plasma matrix.

[0008] As an optional implementation of an embodiment of the present application, based on the absorbance of the primary plasma matrix detected by the optical detection probe, before adjusting the preparation parameters, the method also includes: setting the detection wavelength of the optical detection probe to a preset wavelength range, so that the optical detection probe can detect the absorbance of the primary plasma matrix in real time within the preset wavelength range.

[0009] As an optional implementation of the embodiment of the present application, the multiple tubes of whole blood samples include: a first whole blood sample collected using a solid plasma matrix preparation tube, and a second whole blood sample collected using a liquid plasma matrix preparation tube.

[0010] As an optional implementation of the embodiment of the present application, the initial preparation parameters include: centrifugal force and centrifugal time.

[0011] As an optional implementation of the embodiment of the present application, the liquid level of the primary plasma matrix is ​​located within a preset range below the liquid level of the whole blood sample.

[0012] In a second aspect, the present application provides a device for preparing a plasma matrix, the device comprising:

[0013] An optical detection probe for real-time detection of the absorbance of the plasma matrix;

[0014] A centrifugation module, used for performing preliminary centrifugation on multiple tubes of whole blood samples according to initial preparation parameters to obtain multiple tubes of primary plasma matrix;

[0015] A preparation parameter adjustment module, used for adjusting the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe; wherein there is a linear relationship between the absorbance and the preparation parameters;

[0016] The centrifugation module is also used to centrifuge the primary plasma matrix according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, so as to obtain a solid plasma matrix and a liquid plasma matrix.

[0017] As an optional implementation of the embodiment of the present application, the device also includes: a detection wavelength setting module, which is used to set the detection wavelength of the optical detection probe to be within a preset wavelength range, so that the optical detection probe can detect the absorbance of the primary plasma matrix in real time within the preset wavelength range.

[0018] In a third aspect, the present application provides an electronic device comprising: a processor, a memory, and a computer program stored in the memory and executable on the processor, wherein when the computer program is executed by the processor, the method for preparing a plasma matrix as described in the first aspect or any one of its optional embodiments is implemented.

[0019] In a fourth aspect, the present application provides a computer-readable storage medium, comprising: a computer program is stored on the computer-readable storage medium, and when the computer program is executed by a processor, the method for preparing a plasma matrix as described in the first aspect or any optional embodiment thereof is implemented.

[0020] In a fifth aspect, the present application provides a computer program product, comprising: the computer program product comprises a computer program, and when the computer program is run on a computer, the computer implements the method for preparing a plasma matrix as described in the first aspect or any one of its optional embodiments.

[0021] Compared with the prior art, the technical solution provided by the embodiments of the present application has the following advantages:

[0022] The disclosed embodiments provide a method, device, equipment, medium and program product for preparing a plasma matrix, wherein the method is applied to a plasma matrix preparation platform including an optical detection probe, which is used to detect the absorbance of the plasma matrix in real time. The method first performs preliminary centrifugation on multiple tubes of whole blood samples according to the initial preparation parameters to obtain multiple tubes of primary plasma matrix; then adjust the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe, wherein there is a linear relationship between the absorbance and the preparation parameters; further centrifuge the primary plasma matrix according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, and then obtain a solid plasma matrix and a liquid plasma matrix at the same time. In this way, the embodiment of the present application can ensure the formation of the solid plasma matrix by detecting the absorbance of the plasma matrix in real time and adjusting the preparation parameters according to the linear relationship between the absorbance and the preparation parameters. After the centrifugation, two forms of plasma matrix products are obtained at the same time, which shortens the centrifugation time to a certain extent and improves the preparation efficiency and effect of the plasma matrix. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and, together with the description, serve to explain the principles of the present application.

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0025] Figure 1 A schematic diagram of a process for preparing a plasma matrix provided in an embodiment of the present application;

[0026] Figure 2 This is a comparison chart of the overall preparation time;

[0027] Figure 3 It is a comparison chart of the clotting time of liquid plasma matrix;

[0028] Figure 4 This is a comparison chart of VAS scores of doctor satisfaction;

[0029] Figure 5 A schematic diagram of the structure of a plasma matrix preparation device provided in an embodiment of the present application;

[0030] Figure 6 A schematic diagram of the structure of an electronic device described in an embodiment of the present application. DETAILED DESCRIPTION

[0031] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the technical terms required to be used in the embodiments or the prior art description are briefly introduced below:

[0032] The absorbance of plasma matrix refers to the degree of absorption of light by plasma under the irradiation of light of a specific wavelength. Absorbance is usually represented by the symbol A, and its relationship with transmittance (T) is A=-logT. When light passes through the plasma matrix, various components in the plasma (such as proteins, lipids, metabolites, etc.) will absorb light of a certain wavelength, thereby weakening the intensity of the transmitted light.

[0033] In order to more clearly understand the above-mentioned purposes, features and advantages of the present application, the scheme of the present application will be further described below. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0034] In the following description, many specific details are set forth to facilitate a full understanding of the present application, but the present application may also be implemented in other ways different from those described herein; obviously, the embodiments in the specification are only part of the embodiments of the present application, rather than all of the embodiments.

[0035] In order to solve some or all of the technical problems existing in the related art, the embodiment of the present application provides a method, device, equipment, medium and program product for preparing a plasma matrix, wherein the method is applied to a plasma matrix preparation platform including an optical detection probe, and the optical detection probe is used to detect the absorbance of the plasma matrix in real time. The method first performs preliminary centrifugation on multiple tubes of whole blood samples according to the initial preparation parameters to obtain multiple tubes of primary plasma matrix; then adjust the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe, wherein there is a linear relationship between the absorbance and the preparation parameters; further centrifuge the primary plasma matrix according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, and then obtain solid plasma matrix and liquid plasma matrix at the same time. In this way, the embodiment of the present application can ensure the formation of the solid plasma matrix by detecting the absorbance of the plasma matrix in real time, adjusting the preparation parameters according to the linear relationship between the absorbance and the preparation parameters, and obtaining two forms of plasma matrix products that meet clinical needs at the same time after the centrifugation, shortening the centrifugation time to a certain extent, and improving the preparation efficiency and effect of the plasma matrix.

[0036] A method for preparing a plasma matrix provided in the embodiments of the present application can be implemented by a preparation device or electronic device of the plasma matrix, and the electronic device includes but is not limited to a personal computer, a laptop computer, a tablet computer, a smart phone, etc. The operating system of the electronic device may include Android (Android), a mobile operating system (iOS) developed by Apple, an operating system (Windows) developed by Microsoft Corporation of the United States, etc., and the embodiments of the present application are not limited to this. The electronic device can be operated alone to implement the present application, or it can be connected to the network and implemented by interactive operations with other computer devices in the network. Among them, the network in which the electronic device is located includes but is not limited to the Internet, a wide area network, a metropolitan area network, a local area network, a virtual private network (Virtual Private Network, VPN) network, etc.

[0037] It should be noted that the protection scope of the method for preparing a plasma matrix described in the embodiment of the present application is not limited to the execution order of the steps listed in this embodiment. All solutions implemented by adding, reducing or replacing steps in the prior art based on the principles of the present application are included in the protection scope of the present application.

[0038] like Figure 1 As shown, Figure 1 The present invention is a flow chart of a method for preparing a plasma matrix according to an embodiment of the present invention. The method can be performed by a plasma matrix preparation device, wherein the device can be implemented by software and / or hardware and can generally be integrated in an electronic device. The device or electronic device runs a plasma matrix preparation platform, and the plasma matrix preparation platform includes an optical detection probe for real-time detection of the absorbance of the plasma matrix.

[0039] The method mainly includes the following steps S101 to S103:

[0040] S101. Perform preliminary centrifugation on multiple tubes of whole blood samples according to initial preparation parameters to obtain multiple tubes of primary plasma matrix.

[0041] The initial preparation parameters include centrifugal force and centrifugal time. The initial centrifugal force can be 1-3000g.

[0042] Exemplarily, the initial preparation parameters include a centrifugal force of 2000g-3000g and a centrifugal time of 10-15 minutes, or a centrifugal force of 1000g-1500g and a centrifugal time of 8-12 minutes, etc. This application is only for exemplary description and does not specifically limit the initial preparation parameters.

[0043] In some embodiments, the liquid level of the primary plasma matrix obtained after the initial centrifugation is lower than the liquid level of the whole blood sample and is located within a preset range below the liquid level of the whole blood sample. The preset range can be 2-57 mm. Exemplarily, the liquid level of the primary plasma matrix is ​​2 mm lower than the liquid level of the whole blood sample.

[0044] The multiple tubes of whole blood samples include: a first whole blood sample collected by a solid plasma matrix preparation tube, and a second whole blood sample collected by a liquid plasma matrix preparation tube. The solid plasma matrix preparation tube can be a glass blood collection tube, and the liquid plasma matrix preparation tube can be a plastic blood collection tube. It is understood that venous blood is collected using a solid plasma matrix preparation tube and a liquid plasma matrix preparation tube, respectively.

[0045] Exemplarily, the multiple tubes of whole blood samples include a first whole blood sample collected by 4 glass blood collection tubes and a second whole blood sample collected by 2 plastic blood collection tubes, each of which contains 10 ml of venous blood.

[0046] In some embodiments, the detection wavelength of the optical detection probe is set to a preset wavelength range so that the optical detection probe can detect the absorbance of the primary plasma matrix in real time within the preset wavelength range. The preset wavelength range can be 490-500nm, or 450-480nm. The step of setting the detection wavelength of the optical detection probe can be performed before or at the same time as step S101, but must be performed before step S102.

[0047] Exemplarily, the detection wavelength range of the optical detection probe is set to 490-500 nm, and when the liquid level of the primary plasma matrix in the solid plasma matrix preparation tube is 2 mm lower than the liquid level of the whole blood sample, the absorbance of the primary plasma matrix is ​​detected; another exemplary embodiment, the detection wavelength range of the optical detection probe is set to 450-480 nm, and when the liquid level of the primary plasma matrix in the liquid plasma matrix preparation tube is 7 mm lower than the liquid level of the whole blood sample, the absorbance of the primary plasma matrix is ​​detected.

[0048] S102. Adjust the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe.

[0049] The optical detection probe is used to detect the absorbance of the plasma matrix in real time. There is a linear relationship between the preparation parameters and the absorbance of the primary plasma matrix.

[0050] In some embodiments, during the execution of step S102, the absorbance of the primary plasma matrix detected by the optical detection probe is obtained according to a preset accuracy, and then the preparation parameters are adjusted based on the absorbance.

[0051] Exemplarily, during the execution of step S102, when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D1=2mm, the absorbance detected by the optical detection probe is obtained; when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D2=7mm, the absorbance detected by the optical detection probe is obtained; when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D3=12mm, the absorbance detected by the optical detection probe is obtained; when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D4=17mm, the absorbance detected by the optical detection probe is obtained; when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D5=22mm, the absorbance detected by the optical detection probe is obtained; when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D6=27mm, the absorbance detected by the optical detection probe is obtained. The absorbance detected by the optical detection probe is obtained when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D7 = 32 mm; when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D8 = 37 mm, the absorbance detected by the optical detection probe is obtained; when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D9 = 42 mm, the absorbance detected by the optical detection probe is obtained; when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D10 = 47 mm, the absorbance detected by the optical detection probe is obtained; when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D11 = 52 mm, the absorbance detected by the optical detection probe is obtained; when the liquid level of the primary plasma matrix is ​​lower than the liquid level of the whole blood sample D12 = 57 mm, the absorbance detected by the optical detection probe is obtained. The preparation parameters are then adjusted based on these absorbances.

[0052] In some embodiments, when the absorbance of the detected primary plasma matrix no longer decreases significantly within a preset time, the preparation parameters are adjusted. The preset time can be 30 seconds, and the present application does not specifically limit this. Specifically, the average decrease in the absorbance of the primary plasma matrix within the preset time is calculated, and the average decrease is compared to see whether it is less than the preset decrease. If so, it means that the absorbance no longer decreases significantly within the preset time, and the preparation parameters are adjusted at this time.

[0053] Continuing with the previous example, when it is detected that the absorbance of the primary plasma matrix at D1-D12 no longer decreases significantly within 30 seconds, the initial preparation parameters are adjusted. If the centrifugal force of the initial preparation parameters is 1500g, the adjustment value is 450g.

[0054] Exemplarily, when the monitoring process finds that the absorbance detected by the optical detection probe at the position of 2-57 mm no longer decreases significantly for 30 consecutive seconds, the adjustment parameter is set to the low centrifugal force program, and the centrifugal force is adjusted from the initial value of 1500g to 450g.

[0055] S103. Centrifuge the primary plasma matrix according to the adjusted preparation parameters until the absorbance change trend of the plasma matrix tends to be stable, so as to obtain a solid plasma matrix and a liquid plasma matrix.

[0056] Among them, the solid plasma matrix is ​​a plasma matrix in a gel-like state, and the liquid plasma matrix is ​​a plasma matrix that can maintain a liquid state for more than 60 minutes.

[0057] It should be noted that when centrifugation is not completed, the decrease in the absorbance of the plasma matrix is ​​very obvious, and the absorbance at the same position detected at different times within the preset detection wavelength range will differ by several times. In some embodiments, when the absorbance at D1 to D12 detected by the optical detection probe with a detection wavelength within 490-500nm is stabilized at a minimum value, and the absorbance at D1 to D12 detected by the optical detection probe with a detection wavelength within 450-480nm is stabilized at a maximum value, it indicates that the preparation is complete, and a solid plasma matrix and a liquid plasma matrix are obtained at the same time.

[0058] Exemplarily, when the absorbance at D1~D12 detected by the optical detection probe with a detection wavelength within 490-500nm is stabilized at a minimum value of 1.81, and the absorbance at D1~D12 detected by the optical detection probe with a detection wavelength within 450-480nm is stabilized at a maximum value of 2.30, solid plasma matrix and liquid plasma matrix are obtained at the same time.

[0059] As another example, when the absorbance at D1~D12 detected by the optical detection probe with a detection wavelength within 490-500nm is stabilized at a minimum value of 1.95, and the absorbance at D1~D12 detected by the optical detection probe with a detection wavelength within 450-480nm is stabilized at a maximum value of 2.57, solid plasma matrix and liquid plasma matrix are obtained at the same time.

[0060] It should be noted that the above minimum and maximum values ​​are only exemplary and are not specifically limited in this application.

[0061] In some embodiments, the primary plasma matrix is ​​centrifuged according to the adjusted preparation parameters, and the absorbance of the plasma matrix is ​​continuously detected during the process. Optionally, a graph is drawn based on the absorbance of the detected plasma matrix to show the change trend of the absorbance. The absorbance at different positions corresponds to different absorbance curves with time as the horizontal coordinate and the vertical coordinate as the vertical coordinate, so as to quantitatively analyze the change trend of the absorbance. Optionally, the variance and / or standard deviation of the absorbance of the plasma matrix are calculated. If the variance approaches a smaller value, it means that the absorbance of the plasma matrix tends to be stable. If the standard deviation approaches 0, it means that the absorbance is increasingly concentrated near a fixed value, and the change trend tends to be stable. Optionally, the average rate of change of the absorbance of the plasma matrix within a preset time interval is calculated, or a preset mathematical function is selected for curve fitting. The preset mathematical function can be an exponential function, and the derivative of the fitting curve is used to determine whether the change trend of the absorbance tends to be stable. When the change trend tends to be stable, the centrifugation can be controlled to end, and at this time, two states of plasma matrix can be obtained at the same time.

[0062] In summary, the embodiment of the present application provides a method for preparing a plasma matrix, which is applied to a plasma matrix preparation platform including an optical detection probe, and the optical detection probe is used to detect the absorbance of the plasma matrix in real time. The method first performs preliminary centrifugation on multiple tubes of whole blood samples according to the initial preparation parameters to obtain multiple tubes of primary plasma matrix; then adjust the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe, wherein there is a linear relationship between the absorbance and the preparation parameters; further centrifuge the primary plasma matrix according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, and then obtain solid plasma matrix and liquid plasma matrix at the same time. In this way, the embodiment of the present application can ensure the formation of the solid plasma matrix by detecting the absorbance of the plasma matrix in real time, and adjusting the preparation parameters according to the linear relationship between the absorbance and the preparation parameters. After the centrifugation, two forms of plasma matrix products that meet clinical needs are obtained at the same time, shortening the centrifugation time to a certain extent, and improving the preparation efficiency and effect of the plasma matrix.

[0063] To further reflect the technical effect of the embodiment of the present application, a comparative test was conducted with the preparation method of the existing plasma matrix, using 4 glass blood collection tubes and 2 plastic blood collection tubes to collect 60mL of human blood, each blood collection tube 10mL. The plasma preparation method of the present application and the existing method were applied respectively. The solid plasma matrix coagulation time and liquid plasma matrix coagulation time of the embodiment of the present application and the existing method (as a comparative example) were recorded, and after the operation, for the preparation scheme of this plasma matrix, the doctor was invited to perform visual simulation scoring. The doctor needed to draw a hash mark representing their satisfaction on the unmarked 10 cm line, with the left side marked as "very dissatisfied" and the right side marked as "very satisfied". The satisfaction score is the distance from the left end of the line (in millimeters). In the comparative test, 6 parallel samples were taken for each data, and the test results were averaged.

[0064] Among them, the solid plasma matrix coagulation time is from the time the whole blood is put into the equipment for operation until the solid plasma matrix is ​​completely coagulated. The liquid plasma matrix coagulation time is from the time the solidified solid plasma matrix is ​​obtained to the time the liquid plasma matrix begins to coagulate. It should be noted that a long coagulation time of the liquid plasma matrix will make the coagulation factor detection more accurate, and it is also convenient for multiple items to be jointly tested, which can extend the storage time and reduce the risk of blood transfusion. The Visual Analogue Scale (VAS) is a tool used to assess the degree of pain or other subjective feelings (such as itching, fatigue, etc.). It mainly uses a straight line with a scale to let the subject subjectively express the intensity of his feelings. Using the VAS scale, the subjective feeling information of the subject can be obtained very intuitively.

[0065] Through the above comparative test, Figure 2 The comparison chart of the overall preparation time is shown in Figure 3 Comparison of clotting times of liquid plasma matrices, Figure 4 The comparison chart of the VAS score of doctor satisfaction shown in the figure shows that the overall preparation time of the present application is significantly shortened, the coagulation time of the liquid plasma matrix is ​​longer, and the VAS score of doctor satisfaction is higher.

[0066] like Figure 5 As shown, Figure 5 A schematic diagram of the structure of a plasma matrix preparation device provided in an embodiment of the present application, the device comprising:

[0067] An optical detection probe 501 is used to detect the absorbance of the plasma matrix in real time;

[0068] A centrifugation module 502 is used to perform preliminary centrifugation on multiple tubes of whole blood samples according to initial preparation parameters to obtain multiple tubes of primary plasma matrix;

[0069] The preparation parameter adjustment module 503 is used to adjust the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe 501; wherein there is a linear relationship between the absorbance and the preparation parameters;

[0070] The centrifugation module 502 is further used to centrifuge the primary plasma matrix according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, so as to obtain a solid plasma matrix and a liquid plasma matrix.

[0071] As an optional implementation of the embodiment of the present application, the device also includes: a detection wavelength setting module, which is used to set the detection wavelength of the optical detection probe 501 to be within a preset wavelength range, so that the optical detection probe 501 can detect the absorbance of the primary plasma matrix in real time within the preset wavelength range.

[0072] As an optional implementation of the embodiment of the present application, the multiple tubes of whole blood samples include: a first whole blood sample collected using a solid plasma matrix preparation tube, and a second whole blood sample collected using a liquid plasma matrix preparation tube.

[0073] As an optional implementation of the embodiment of the present application, the initial preparation parameters include: centrifugal force and centrifugal time.

[0074] As an optional implementation of the embodiment of the present application, the liquid level of the primary plasma matrix is ​​located within a preset range below the liquid level of the whole blood sample.

[0075] For the specific definition of the preparation device of the plasma matrix, please refer to the definition of the preparation method of the plasma matrix above, which will not be repeated here. Each module in the preparation device of the plasma matrix can be implemented in whole or in part by software, hardware and a combination thereof. The above modules can be embedded in or independent of the processor in the computer device in the form of hardware, or can be stored in the memory of the computer device in the form of software, so that the processor can call and execute the operations corresponding to the above modules.

[0076] In one embodiment, the present application provides an electronic device, which may be a terminal, and its internal structure diagram may be as follows: Figure 6As shown. The electronic device includes a processor, a memory, a communication interface, a display screen and an input device connected by a system bus. Among them, the processor of the electronic device is used to provide computing and control capabilities. The memory of the electronic device includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system and a computer program. The internal memory provides an environment for the operation of the operating system and the computer program in the non-volatile storage medium. The communication interface of the electronic device is used to communicate with an external terminal in a wired or wireless manner, and the wireless manner can be implemented through WIFI, an operator network, near field communication (NFC) or other technologies. When the computer program is executed by the processor, a jam detection method is implemented. The display screen of the electronic device can be a liquid crystal display screen or an electronic ink display screen, and the input device of the electronic device can be a touch layer covered on the display screen, or a button, a trackball or a touchpad provided on the housing of the electronic device, or an external keyboard, touchpad or mouse, etc.

[0077] Those skilled in the art will understand that Figure 6 The structure shown in the figure is merely a block diagram of a partial structure related to the scheme of the present application, and does not constitute a limitation on the electronic device to which the scheme of the present application is applied. The specific electronic device may include more or fewer components than shown in the figure, or combine certain components, or have a different arrangement of components.

[0078] In one embodiment, the plasma matrix preparation device provided in the present application can be implemented in the form of a computer program, and the computer program can be used in Figure 6 The memory of the electronic device may store various program modules constituting the plasma matrix preparation device, for example, Figure 5 The optical detection probe 501, the centrifugation module 502 and the preparation parameter adjustment module 503 are shown. The computer program composed of various program modules enables the processor to execute the steps of the method for preparing the plasma matrix of each embodiment of the present application described in this specification.

[0079] For example, Figure 6 The electronic device shown can be Figure 5The optical detection probe 501 in the plasma matrix preparation device shown performs real-time detection of the absorbance of the plasma matrix; the electronic device can use the centrifugation module 502 to perform preliminary centrifugation on multiple tubes of whole blood samples according to the initial preparation parameters to obtain multiple tubes of primary plasma matrix; the electronic device can use the preparation parameter adjustment module 503 to adjust the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe 501; wherein there is a linear relationship between the absorbance and the preparation parameters; the electronic device can use the centrifugation module 502 to centrifuge the primary plasma matrix according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, so as to obtain a solid plasma matrix and a liquid plasma matrix.

[0080] In one embodiment, the present application provides an electronic device, including a memory and a processor, wherein the memory stores a computer program, and when the processor executes the computer program, the following steps are implemented:

[0081] According to the initial preparation parameters, multiple tubes of whole blood samples are preliminarily centrifuged to obtain multiple tubes of primary plasma matrices; the preparation parameters are adjusted according to the absorbance of the primary plasma matrix detected by the optical detection probe; wherein there is a linear relationship between the absorbance and the preparation parameters; the primary plasma matrix is ​​centrifuged according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, so as to obtain a solid plasma matrix and a liquid plasma matrix.

[0082] In one embodiment, the processor also implements the following steps when executing the computer program: before adjusting the preparation parameters based on the absorbance of the primary plasma matrix detected by the optical detection probe, the method also includes: setting the detection wavelength of the optical detection probe to a preset wavelength range, so that the optical detection probe detects the absorbance of the primary plasma matrix in real time within the preset wavelength range.

[0083] In one embodiment, when the processor executes the computer program, the following steps are further implemented: the multiple tubes of whole blood samples include: a first whole blood sample collected using a solid plasma matrix preparation tube, and a second whole blood sample collected using a liquid plasma matrix preparation tube.

[0084] In one embodiment, when the processor executes the computer program, the following steps are also implemented: the initial preparation parameters include: centrifugal force and centrifugal time.

[0085] In one embodiment, when the processor executes the computer program, the following steps are further implemented: the liquid level of the primary plasma matrix is ​​located within a preset range below the liquid level of the whole blood sample.

[0086] When the processor in the electronic device provided by the present application executes the computer program, firstly, the multi-tube whole blood samples are preliminarily centrifuged according to the initial preparation parameters to obtain multi-tube primary plasma matrices; then, according to the absorbance of the primary plasma matrix detected by the optical detection probe, the preparation parameters are adjusted, wherein there is a linear relationship between the absorbance and the preparation parameters; further, the primary plasma matrix is ​​centrifuged according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, and a solid plasma matrix and a liquid plasma matrix are obtained at the same time. In this way, the embodiment of the present application can ensure the formation of the solid plasma matrix by detecting the absorbance of the plasma matrix in real time and adjusting the preparation parameters according to the linear relationship between the absorbance and the preparation parameters. After the centrifugation, two forms of plasma matrix products that meet clinical needs are obtained at the same time, which shortens the centrifugation time to a certain extent and improves the preparation efficiency and effect of the plasma matrix.

[0087] In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored, and when the computer program is executed by the computer program, the following steps are implemented:

[0088] According to the initial preparation parameters, multiple tubes of whole blood samples are preliminarily centrifuged to obtain multiple tubes of primary plasma matrices; the preparation parameters are adjusted according to the absorbance of the primary plasma matrix detected by the optical detection probe; wherein there is a linear relationship between the absorbance and the preparation parameters; the primary plasma matrix is ​​centrifuged according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, so as to obtain a solid plasma matrix and a liquid plasma matrix.

[0089] In one embodiment, the computer program further implements the following steps when executing the computer program: before adjusting the preparation parameters based on the absorbance of the primary plasma matrix detected by the optical detection probe, the method also includes: setting the detection wavelength of the optical detection probe to a preset wavelength range, so that the optical detection probe detects the absorbance of the primary plasma matrix in real time within the preset wavelength range.

[0090] In one embodiment, when the computer program is executed, the computer program further implements the following steps: the multiple tubes of whole blood samples include: a first whole blood sample collected using a solid plasma matrix preparation tube, and a second whole blood sample collected using a liquid plasma matrix preparation tube.

[0091] In one embodiment, the computer program further implements the following steps when executing the computer program: the initial preparation parameters include: centrifugal force and centrifugal time.

[0092] In one embodiment, the computer program further implements the following steps when executing the computer program: the liquid level of the primary plasma matrix is ​​located within a preset range below the liquid level of the whole blood sample.

[0093] When the computer program in the computer-readable storage medium provided by the present application executes the computer program, first, the multiple tubes of whole blood samples are preliminarily centrifuged according to the initial preparation parameters to obtain multiple tubes of primary plasma matrix; then, according to the absorbance of the primary plasma matrix detected by the optical detection probe, the preparation parameters are adjusted, wherein there is a linear relationship between the absorbance and the preparation parameters; further, the primary plasma matrix is ​​centrifuged according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, and a solid plasma matrix and a liquid plasma matrix are obtained at the same time. In this way, the embodiment of the present application can ensure the formation of the solid plasma matrix by detecting the absorbance of the plasma matrix in real time and adjusting the preparation parameters according to the linear relationship between the absorbance and the preparation parameters. After the centrifugation, two forms of plasma matrix products that meet clinical needs are obtained at the same time, which shortens the centrifugation time to a certain extent and improves the preparation efficiency and effect of the plasma matrix.

[0094] Those skilled in the art will appreciate that the embodiments of the present application may be provided as methods, systems, or computer program products. Therefore, the present application may adopt the form of a complete hardware embodiment, a complete software embodiment, or an embodiment combining software and hardware. Moreover, the present application may adopt the form of a computer program product implemented on one or more computer-usable storage media that include computer-usable program code.

[0095] In several embodiments provided by the present application, it should be understood that the disclosed devices and methods can also be implemented in other ways. The device embodiments described above are merely schematic. For example, the flowcharts and block diagrams in the accompanying drawings show the possible architecture, functions and operations of the devices, methods and computer program products according to multiple embodiments of the present application. In this regard, each box in the flowchart or block diagram can represent a module, a program segment or a part of a code, and the module, a program segment or a part of a code contains one or more executable instructions for implementing the specified logical function. It should also be noted that in some alternative implementations, the functions marked in the box can also occur in a different order from the order marked in the accompanying drawings. For example, two consecutive boxes can actually be executed substantially in parallel, and they can sometimes be executed in the opposite order, depending on the functions involved. It should also be noted that each box in the block diagram and / or flowchart, and the combination of boxes in the block diagram and / or flowchart can be implemented with a dedicated hardware-based system that performs a specified function or action, or can be implemented with a combination of dedicated hardware and computer instructions.

[0096] In the present application, the processor may be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. A general-purpose processor may be a microprocessor or the processor may also be any conventional processor, etc.

[0097] In this application, memory may include non-permanent memory in a computer-readable medium, random access memory (RAM) and / or non-volatile memory, such as read-only memory (ROM) or flash RAM. Memory is an example of a computer-readable medium.

[0098] In this application, computer-readable media includes permanent and non-permanent, removable and non-removable storage media. Storage media can be implemented by any method or technology to store information, and the information can be computer-readable instructions, data structures, modules of programs or other data. Examples of computer storage media include, but are not limited to, phase change memory (PRAM), static random access memory (SRAM), dynamic random access memory (DRAM), other types of random access memory (RAM), read-only memory (ROM), electrically erasable programmable read-only memory (EEPROM), flash memory or other memory technology, read-only compact disk read-only memory (CD-ROM), digital versatile disk (DVD) or other optical storage, magnetic cassettes, disk storage or other magnetic storage devices or any other non-transmission media that can be used to store information that can be accessed by a computing device. According to the definition in this article, computer-readable media does not include temporary computer-readable media (transitory media), such as modulated data signals and carrier waves.

[0099] It should be noted that, in this article, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0100] The above is only a specific implementation of the present application, so that those skilled in the art can understand or implement the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to these embodiments herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for preparing a plasma matrix, characterized in that: Applied to a plasma matrix preparation platform, the plasma matrix preparation platform includes an optical detection probe for real-time detection of the absorbance of the plasma matrix, the method comprising: Multiple tubes of whole blood samples were initially centrifuged according to initial preparation parameters to obtain multiple tubes of primary plasma matrix; Adjusting the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe; wherein there is a linear relationship between the absorbance and the preparation parameters; The primary plasma matrix is ​​centrifuged according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, so as to obtain a solid plasma matrix and a liquid plasma matrix.

2. The method according to claim 1, characterized in that Before adjusting the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe, the method further comprises: The detection wavelength of the optical detection probe is set within a preset wavelength range so that the optical detection probe detects the absorbance of the primary plasma matrix in real time within the preset wavelength range.

3. The method according to claim 1, characterized in that The multiple tubes of whole blood samples include: a first whole blood sample collected using a solid plasma matrix preparation tube, and a second whole blood sample collected using a liquid plasma matrix preparation tube.

4. The method according to claim 1, characterized in that: The initial preparation parameters include: centrifugal force and centrifugal time.

5. The method according to claim 1, characterized in that: The liquid level of the primary plasma matrix is ​​within a preset range below the liquid level of the whole blood sample.

6. A plasma matrix preparation device, characterized in that: include: An optical detection probe for real-time detection of the absorbance of the plasma matrix; A centrifugation module, used for performing preliminary centrifugation on multiple tubes of whole blood samples according to initial preparation parameters to obtain multiple tubes of primary plasma matrix; A preparation parameter adjustment module, used to adjust the preparation parameters according to the absorbance of the primary plasma matrix detected by the optical detection probe; wherein there is a linear relationship between the absorbance and the preparation parameters; The centrifugation module is also used to centrifuge the primary plasma matrix according to the adjusted preparation parameters until the change trend of the absorbance of the plasma matrix tends to be stable, so as to obtain a solid plasma matrix and a liquid plasma matrix.

7. The device according to claim 6, characterized in that The device also includes: The detection wavelength setting module is used to set the detection wavelength of the optical detection probe to be within a preset wavelength range, so that the optical detection probe can detect the absorbance of the primary plasma matrix in real time within the preset wavelength range.

8. An electronic device, characterized in that: include: A processor, a memory, and a computer program stored in the memory and executable on the processor, wherein when the computer program is executed by the processor, the method for preparing a plasma matrix according to any one of claims 1 to 5 is implemented.

9. A computer-readable storage medium, characterized in that: include: The computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the method for preparing a plasma matrix according to any one of claims 1 to 5 is implemented.

10. A computer program product, characterized in that include: The computer program product comprises a computer program, and when the computer program is run on a computer, the computer is caused to implement the method for preparing the plasma matrix according to any one of claims 1 to 5.

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