Method for improving economic benefits of artemisia lavandulatum in remediation of soil copper pollution and application of method

Abstract

The invention discloses a method for improving the economic benefit of restoring soil copper pollution by artemisia lavandulatum and application of the method. The method comprises the following steps: S1, selecting artemisia lavandulatum naturally growing in a copper mine copper pollution area; s2, preparation of an arbuscular mycorrhizal fungus inoculant; s3, adopting a cuttage mode to obtain artemisia lavandulatum seedlings; s4, soil treatment, wherein bacterial manure is fully mixed with the to-be-remediated soil according to the mass ratio of 3%-4%; s5, transplanting artemisia lavandulatum seedlings into the soil treated in the step S4 for culture; s6, harvesting and extracting essential oil, namely at the last stage of the growth season of the Artemisia laevigata, harvesting Artemisia laevigata stalks flush with the ground, taking away deadwood and residual leaves, extracting the essential oil from the harvested Artemisia laevigata stalks, preparing biomass fuel from residues after essential oil extraction and the deadwood and residual leaves, and performing centralized treatment on ash after combustion. According to the method, the damage of copper to the Artemisia laevigata is reduced, the Artemisia laevigata extracted essential oil is obtained, and high economic benefits can be obtained while the polluted site is repaired.

Description

Technical Field

[0001] The invention relates to the technical field of heavy metal copper pollution control, and in particular to a method for improving the economic benefit of Artemisia argyi in repairing soil copper pollution and an application thereof. Background Art

[0003] At present, there are three main methods used to repair copper-contaminated soil: physical remediation, chemical remediation and biological remediation. Compared with physical remediation and chemical remediation, biological remediation is more economical, green and environmentally friendly, and is considered to be almost the only reasonable choice for repairing copper-contaminated soil.

[0004] Artemisia argyi, a plant of the Asteraceae family in my country, is widely distributed, has a well-developed root system, grows rapidly, has a large biomass, and is highly resistant. It is distributed in many copper tailings. Therefore, even if it is not a hyperaccumulator, it can play a major role in the phytoremediation of heavy metal contaminated soils. At the same time, Artemisia argyi is an aromatic plant with high economic added value.

[0005] Arbuscular mycorrhizal fungi (AMF) are a type of beneficial endomycorrhizal fungi in the soil that can be used to reduce heavy metal accumulation in crops. However, there are few studies on the combined restoration of copper and heavy metal pollution by inoculating mugwort with arbuscular mycorrhizal fungi, and the effect of AMF inoculation on mugwort to restore copper-contaminated soil is still unknown. Summary of the invention

[0006] The purpose of the present invention is to provide a method for improving the economic benefit of using Artemisia argyi to repair soil copper pollution in soil contaminated by copper from ore washing wastewater and mining waste residues and its application. The method reduces the damage of copper to Artemisia argyi and obtains Artemisia argyi essential oil extraction at the same time. Higher economic benefits can be obtained while repairing the contaminated site, which well solves the problem that Artemisia argyi has not yet been used in the prior art to inoculate arbuscular mycorrhizal fungi to jointly repair copper and heavy metal pollution.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a method for improving the economic benefits of remediating soil copper pollution by Artemisia argyi, comprising the following steps:

[0009] S1. Variety selection: Use wild mugwort that grows naturally in the copper-contaminated area of ​​the copper mine;

[0010] S2. Preparation of arbuscular mycorrhizal fungal inoculant: inoculate arbuscular mycorrhizal fungal species into a propagation matrix, mix thoroughly, place in an independent propagation container, and inoculate with sterilized and cleaned germinated corn seeds, and after culturing for 12 to 16 weeks, remove the above-ground parts of the corn plants, cut the underground roots of the corn plants into pieces, mix thoroughly with the propagation matrix, naturally air-dry, and crush to form an inoculant;

[0011] S3, Artemisia argyi seedling propagation: Artemisia argyi seedlings are obtained by cutting, the Artemisia argyi obtained in step S1 is used as cuttings, the bottom of the cuttings is placed in a disinfectant and soaked for 10 min to 15 min after pruning, the bottom of the cuttings is placed in a rooting solution and soaked for 2 h to 3 h after disinfection, and then taken out to dry, and after drying, the cuttings are inserted into a seedling breeding matrix and maintained for 50 to 60 days;

[0012] S4, soil treatment: evenly spread the bacterial agent obtained in step S2 on the surface of the soil to be repaired, so that the bacterial agent and the soil are fully mixed, and the amount of the bacterial agent added is 3% to 4% of the weight of the soil;

[0013] S5, transplanting and daily maintenance: transplanting the Artemisia argyi seedlings into the soil treated in step S4 for cultivation, and watering and fertilizing regularly;

[0014] S6. Harvesting and extracting essential oils: At the end of the Artemisia argyi growing season, the Artemisia argyi stems are harvested at ground level, and the dead branches and leaves are taken away. The harvested Artemisia argyi stems are used for extracting essential oils by steam distillation. The residues after essential oil extraction are used together with the dead branches and leaves to make biomass fuel, and the ash after combustion is processed centrally.

[0015] As a specific technical solution of the present invention, in step S2, the propagation matrix is ​​obtained by the following steps: sand and soil are mixed in a volume ratio of 1:1 or sand, soil, vermiculite and zeolite are mixed in a volume ratio of 2:1:1:1, and then sterilized under 121°C steam for 90min to 100min, and cooled for use.

[0016] As a specific technical solution of the present invention, in step S2, the breeding container adopts a common container such as a plastic flower pot with a volume of 3L.

[0017] As a specific technical solution of the present invention, in step S2, the arbuscular mycorrhizal fungus is Glomus mosseae, the weight ratio of the fungus species to the propagation matrix is ​​1:3 or 1:4, and the number of spores in the formed bacterial agent is 100-150 / 10g soil.

[0018] As a specific technical solution of the present invention, in step S2, after the first true leaf of the corn seed unfolds (about 8 to 10 days after sowing), water the seed with low-phosphorus Hoagland nutrient solution (specifically, 1 / 4 phosphorus modified Hoagland nutrient solution can be used) once a week for three consecutive weeks; thereafter, water the seed with low-phosphorus Hoagland nutrient solution every day until the 12th to 16th week.

[0019] As a specific technical solution of the present invention, in step S3, branches with full axillary buds and one full bud are selected as cuttings, and the length of the cuttings is 4 to 6 cm; the disinfectant is a 1000-fold solution of 25% carbendazim; the rooting solution is a 100 mg / L ABT aqueous solution; the seedling breeding matrix includes peat soil, perlite and river sand mixed in a volume ratio of 3:1:1; the curing conditions are placed in a black net arch shed with an atmospheric temperature of 25°C to 28°C and a relative air humidity of 90%, and low-phosphorus Hoagland nutrient solution is watered during curing, and the watering amount of the low-phosphorus Hoagland nutrient solution each time is 150 to 250 ml.

[0020] As a specific technical solution of the present invention, in step S5, after the Artemisia argyi seedlings are transplanted, they are placed in an environment with an atmospheric temperature of 25°C to 30°C and a relative air humidity of 55% to 70% for maintenance, 5 plants are transplanted per pot, 3 kg of soil is filled in each pot, and after transplanting, low-phosphorus Hoagland nutrient solution is watered once a week, and the watering amount is 150 to 250 ml per pot.

[0021] As a specific technical solution of the present invention, in step S6, essential oil refining includes the following steps: drying→crushing→immersing→extraction→still stratification of distillate→filtration→washing with anhydrous sodium sulfate→drying.

[0022] Furthermore, the specific essential oil extraction operation is as follows: after the Artemisia argyi stems are naturally air-dried and crushed, the Artemisia argyi stems are soaked in distilled water, cold-soaked and then heated for extraction by steam distillation, and after reflux, the Artemisia argyi essential oil is separated, and a small amount of desiccant anhydrous sodium sulfate is added to obtain an essential oil product; wherein, the mass ratio of Artemisia argyi stems to distilled water is 1:10, the soaking time is 1h, the distillation time is 1.5h, the reflux time is 4h, and the extraction rate of essential oil is 0.2% to 0.25%.

[0023] In a second aspect, the present invention also provides an application of the above-mentioned method for improving the economic benefits of Artemisia argyi in repairing soil copper pollution in repairing soil copper pollution.

[0024] Furthermore, the copper content in the soil is 22.3 mg / kg to 800 mg / kg.

[0025] As a specific technical solution of the present invention, for example, the copper content in the soil is 22.3 mg / kg; or, the copper content in the soil is 200 mg / kg; or, the copper content in the soil is 400 mg / kg; or, the copper content in the soil is 800 mg / kg.

[0026] Compared with the prior art, the present invention provides a method for improving the economic benefits of remediating soil copper pollution with Artemisia argyi and its application, which has the following beneficial effects:

[0027] (1) The present invention utilizes Glomus mosseae to form a symbiosis with Artemisia argyi to produce a huge extraradical mycelial system, which combines with Cu in the soil and prevents it from being transported to the roots, thereby reducing the absorption of Cu by the plant roots, alleviating the damage of Cu to the plants, and increasing the dry weight of Artemisia argyi plants. However, Artemisia argyi can still adsorb and reduce the copper content in the soil, thereby having the effect of repairing contaminated soil.

[0028] (2) The boiling point of copper is 2567°C. Therefore, the Artemisia argyi essential oil extracted by heating with steam distillation does not contain copper heavy metals. The Artemisia argyi essential oil is extracted by steam distillation, and the extraction rate of Artemisia argyi essential oil is about 0.23%, which can increase the economic value in the process of copper-contaminated soil remediation. DETAILED DESCRIPTION

[0029] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] In the examples and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, strains, reagents, etc. used are all commercially available unless otherwise specified.

[0031] The present invention is further described in detail below through detailed examples.

[0032] Example 1

[0033] The test site is located in Songshan Lake District, Dongguan City, which has a subtropical monsoon climate, long summer and no winter, sufficient sunshine, abundant rainfall, small temperature difference amplitude, obvious monsoon, annual average temperature above 20℃, annual average sunshine of 1800-2000h, frost-free period, and good site conditions suitable for the growth of wild mugwort.

[0034] This embodiment provides a method for improving the economic benefits of remediating soil copper pollution with Artemisia argyi, comprising the following steps:

[0035] S1. Variety selection: Use wild mugwort (Latin name: Artemisia lavandulaefolia DC) that grows naturally in the copper-contaminated area of ​​copper mine tailings. It is a perennial herb with upright stems.

[0036] S2. Preparation of arbuscular mycorrhizal fungal inoculant: inoculate arbuscular mycorrhizal fungal species (specifically Glomus mosesi) into the propagation matrix, with the weight ratio of the species to the propagation matrix being 1:4, and put into an independent propagation container (a 3L plastic flower pot) after thorough mixing, and inoculate with sterilized and cleaned germinated corn seeds; after the first true leaf of the corn seeds unfolds (approximately 8 to 10 days after sowing), water once a week with low-phosphorus Hoagland nutrient solution (specifically, 1 / 4 phosphorus modified Hoagland nutrient solution can be used) for three consecutive weeks, and then water with low-phosphorus Hoagland nutrient solution every day until the 16th week of cultivation, remove the above-ground part of the corn plant, cut the underground roots of the corn plant into pieces, mix them thoroughly with the propagation matrix, air-dry naturally, and crush to form an inoculant, with the number of spores in the inoculant being 100 to 150 per 10g of soil. The propagation matrix is ​​obtained by the following steps: sand, soil, vermiculite and zeolite are fully mixed in a volume ratio of 2:1:1:1, then sterilized under 121° C. steam for 100 minutes, and then cooled for use.

[0037] S3, Artemisia argyi seedling propagation: Artemisia argyi seedlings are obtained by cutting, the Artemisia argyi obtained in step S1 is used as cuttings, branches with full axillary buds and one full bud are selected as cuttings, and the length of the cuttings is 4-6 cm; after pruning, the bottom end of the cuttings is placed in a disinfectant (1000 times solution of 25% carbendazim) and soaked for 15 minutes, after disinfection, the bottom end of the cuttings is placed in a rooting solution (100 mg / L ABT aqueous solution) and soaked for 2 hours, then taken out and dried, and after drying, the cuttings are inserted into a seedling breeding matrix (peat soil, perlite and river sand mixed in a volume ratio of 3:1:1), placed in a black net arch shed with an atmospheric temperature of 25°C to 28°C and a relative air humidity of 90% for maintenance for 60 days, and watered with low-phosphorus Hogland nutrient solution during maintenance, and the amount of each watering of the low-phosphorus Hogland nutrient solution is 150-250 ml.

[0038] S4, soil treatment: evenly spread the bacterial agent obtained in step S2 on the surface of the soil to be repaired, so that the bacterial agent and the soil are fully mixed, and the amount of the bacterial agent added is 3% of the weight of the soil. In order to facilitate the experiment, the soil to be repaired uses garden soil that has been treated with poison. The initial soil organic matter content in the garden soil is 12.5%, the pH value is 6.63, and the copper content is 22.3 mg / kg. After the garden soil is treated with poison, the copper content is 200 mg / kg.

[0039] S5, transplanting and daily maintenance: transplant the Artemisia argyi seedlings into plastic pots, fill each pot with 3 kg of soil treated in step S4 as planting soil, transplant 5 seedlings of similar size into each pot, set 3 repetitions, each repetition has 5 pots, and 75 seedlings are required. After transplanting the Artemisia argyi seedlings, place them in an environment with an atmospheric temperature of 25°C to 30°C and a relative air humidity of 55% to 70% for maintenance, and water them once a week with low-phosphorus Hoagland nutrient solution, with an irrigation amount of 150 to 250 ml / pot.

[0040] S6. Harvest and extract essential oil: At the end of the Artemisia argyi growing season, harvest Artemisia argyi stems at ground level, and take away the dead branches and leaves. The harvested Artemisia argyi stems are extracted with steam distillation to extract essential oil. The residue after essential oil extraction is used together with the dead branches and leaves to make biomass fuel, and the ash after combustion is centrally processed. Among them, essential oil extraction includes the following steps: drying → crushing → soaking → extraction → static stratification of distillate → filtration → washing with anhydrous sodium sulfate → drying. The specific essential oil extraction operation is: after the Artemisia argyi stems are naturally air-dried and crushed, soak the Artemisia argyi stems in distilled water for 1 hour at a mass ratio of 1:10, and then heat and extract them by steam distillation for 1.5 hours after cold soaking. After 4 hours of reflux, separate the Artemisia argyi essential oil, add a little desiccant anhydrous sodium sulfate, and obtain the essential oil product. The extraction rate of essential oil is 0.23%.

[0041] Example 2

[0042] The difference from Example 1 is that in the soil treatment process of step S4, after the garden soil is treated with poison, the copper content in the soil is 400 mg / kg.

[0043] Example 3

[0044] The difference from Example 1 is that in the soil treatment process of step S4, after the garden soil is treated with poison, the copper content in the soil is 800 mg / kg.

[0045] Comparative Example 1

[0046] The difference from Example 1 is that the bacterial agent obtained in step S2 is not added during the soil treatment process in step S4.

[0047] Comparative Example 2

[0048] The difference from Example 2 is that the bacterial agent obtained in step S2 is not added during the soil treatment process in step S4.

[0049] Comparative Example 3

[0050] The difference from Example 3 is that the bacterial agent obtained in step S2 is not added during the soil treatment process in step S4.

[0051] The Cu content in the roots, stems and leaves of Artemisia argyi was detected by using Examples 1 to 3 and Comparative Examples 1 to 3, and the data are shown in Table 1 below.

[0052] Table 1 Copper content in roots, stems and leaves of Artemisia argyi, dry weight per plant and copper absorption per plant

[0053]

[0054] As shown in Table 1, compared with Comparative Examples 1 to 3, the Cu content in the roots, stems and leaves of Artemisia argyi in Examples 1 to 3 is lower, the dry weight of a single plant is increased, and the copper absorption of a single plant is reduced, indicating that the application of arbuscular mycorrhizal fungi under Cu pollution of 200 mg / kg, 400 mg / kg and 800 mg / kg can reduce the absorption of copper by Artemisia argyi and reduce the damage of copper to Artemisia argyi.

[0055] The above embodiments are merely illustrative of the concept and technical solution of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or change the above embodiments without violating the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by a person with ordinary knowledge in the technical field without departing from the spirit and technical ideas disclosed by the present invention should still be covered by the claims of the present invention.

[0056] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each implementation mode may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A method for improving the economic benefits of remediating soil copper pollution by Artemisia argyi, characterized in that: The steps include: S1. Variety selection: Use wild mugwort that grows naturally in the copper-contaminated area of ​​the copper mine; S2. Preparation of arbuscular mycorrhizal fungal inoculant: inoculate arbuscular mycorrhizal fungal species into a propagation matrix, mix thoroughly, place in an independent propagation container, and inoculate with sterilized and cleaned germinated corn seeds, and after culturing for 12 to 16 weeks, remove the above-ground parts of the corn plants, cut the underground roots of the corn plants into pieces, mix thoroughly with the propagation matrix, naturally air-dry, and crush to form an inoculant; S3, Artemisia argyi seedling propagation: Artemisia argyi seedlings are obtained by cutting, the Artemisia argyi obtained in step S1 is used as cuttings, the bottom of the cuttings is placed in a disinfectant and soaked for 10 min to 15 min after pruning, the bottom of the cuttings is placed in a rooting solution and soaked for 2 h to 3 h after disinfection, and then taken out to dry, and after drying, the cuttings are inserted into a seedling breeding matrix and maintained for 50 to 60 days; S4, soil treatment: evenly spread the bacterial agent obtained in step S2 on the surface of the soil to be repaired, so that the bacterial agent and the soil are fully mixed, and the amount of the bacterial agent added is 3% to 4% of the weight of the soil; S5, transplanting and daily maintenance: transplanting the Artemisia argyi seedlings into the soil treated in step S4 for cultivation, and watering and fertilizing regularly; S6. Harvesting and extracting essential oils: At the end of the Artemisia argyi growing season, the Artemisia argyi stems are harvested at ground level, and the dead branches and leaves are taken away. The harvested Artemisia argyi stems are used for extracting essential oils by steam distillation. The residues after essential oil extraction are used together with the dead branches and leaves to make biomass fuel, and the ash after combustion is processed centrally.

2. The method for improving the economic benefits of remediating soil copper pollution by Artemisia argyi according to claim 1, characterized in that: In step S2, the propagation matrix is ​​obtained by the following steps: sand and soil are mixed in a volume ratio of 1:1 or sand, soil, vermiculite and zeolite are mixed in a volume ratio of 2:1:1:1, and then sterilized under 121°C steam for 90min to 100min, and then cooled for use.

3. The method for improving the economic benefits of remediating soil copper pollution by Artemisia argyi according to claim 1, characterized in that: In step S2, the arbuscular mycorrhizal fungus is Glomus mosseae, the weight ratio of the fungus species to the propagation matrix is ​​1:3 or 1:4, and the number of spores in the formed bacterial agent is 100-150 / 10g soil.

4. The method for improving the economic benefits of remediating soil copper pollution by Artemisia argyi according to claim 1, characterized in that: In step S2, after the first true leaf of the corn seeds unfolds, water the seeds with low-phosphorus Hoagland nutrient solution once a week for three consecutive weeks; thereafter, water the seeds with low-phosphorus Hoagland nutrient solution every day until the 12th to 16th week.

5. The method for improving the economic benefits of remediating soil copper pollution by Artemisia argyi according to claim 1, characterized in that: In step S3, branches with full axillary buds and one full bud are selected as cuttings, and the length of the cuttings is 4-6 cm; the disinfectant is a 1000-fold solution of 25% carbendazim; the rooting solution is a 100 mg / L ABT aqueous solution; the seedling breeding matrix includes peat soil, perlite and river sand mixed in a volume ratio of 3:1:1; the curing conditions are to place the seedlings in a black net arch shed with an atmospheric temperature of 25°C to 28°C and a relative air humidity of 90%, and to water them with low-phosphorus Hoagland nutrient solution during curing, and the watering amount of the low-phosphorus Hoagland nutrient solution each time is 150-250 ml.

6. The method for improving the economic benefits of remediating soil copper pollution by Artemisia argyi according to claim 1, characterized in that: In step S5, after the Artemisia argyi seedlings are transplanted, they are placed in an environment with an atmospheric temperature of 25°C to 30°C and a relative air humidity of 55% to 70% for maintenance. Five plants are transplanted into each pot, and each pot is filled with 3kg of soil. After transplanting, the seedlings are irrigated once a week with low-phosphorus Hoagland nutrient solution, and the irrigation amount is 150 to 250 ml per pot.

7. The method for improving the economic benefits of remediating soil copper pollution by Artemisia argyi according to claim 1, characterized in that: In step S6, the essential oil refining includes the following steps: drying→crushing→immersing→extraction→still stratification of distillate→filtration→washing with anhydrous sodium sulfate→drying.

8. The method for improving the economic benefits of remediating soil copper pollution by Artemisia argyi according to claim 1, characterized in that: The specific essential oil extraction operation is as follows: after the Artemisia argyi stems are naturally air-dried and crushed, the Artemisia argyi stems are soaked in distilled water, cold-soaked and then heated for extraction by steam distillation, after reflux, the Artemisia argyi essential oil is separated, and anhydrous sodium sulfate, a desiccant, is added to obtain an essential oil product; wherein, the mass ratio of Artemisia argyi stems to distilled water is 1:10, the soaking time is 1 hour, the distillation time is 1.5 hours, the reflux time is 4 hours, and the extraction rate of essential oil is 0.2% to 0.25%.

9. Use of the method for improving the economic benefits of remediating soil copper pollution by Artemisia argyi as claimed in any one of claims 1 to 8 in remediating soil copper pollution.

10. The use according to claim 9, characterized in that: The copper content in the soil is 22.3 mg / kg to 800 mg / kg.

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