Repair polypeptide SC17-2 and application thereof in skin repair

Abstract

The invention belongs to the technical field of polypeptides, and particularly relates to a repair polypeptide SC17-2 and application thereof in skin repair. The repair polypeptide SC17-2 provided by the invention comprises an amino acid sequence as shown in SEQ ID NO: 1. The repairing polypeptide SC17-2 has multiple effects of repairing skin wounds, improving skin antioxidant activity and promoting skin angiogenesis, so that a more excellent skin repairing function is achieved. Meanwhile, the repairing polypeptide SC17-2 has good chemical stability, is high in adaptability to different environmental conditions and is easy to industrially synthesize.

Description

Technical Field

[0001] The present invention belongs to the technical field of polypeptides, and in particular relates to a repair polypeptide SC17-2 and an application thereof in skin repair. Background Art

[0002] The skin is an organ that covers the surface of the body and is in direct contact with the external environment. It has the functions of protection, excretion, regulating body temperature and sensing external stimuli. It can resist the invasion of pathogens on the surface of the skin and plays a very important protective role in human health. Once the skin is damaged, the skin barrier is destroyed, causing the skin to lose its normal protective function. Pathogens on the surface of the skin can easily enter the skin through the damaged skin barrier, thereby further triggering a series of pathological reactions including skin inflammation.

[0003] Skin repair has long been a concern in the field. Skin repair mainly includes skin repair after trauma, improving the skin's antioxidant function and promoting angiogenesis, and there are some targeted repair schemes in the prior art. For skin repair after trauma, there are some substances or methods for skin repair and related functions in the prior art, such as growth factor substances used in some studies. Growth factors bind to receptors on the cell surface and trigger signal transduction pathways in cells, thereby promoting cell proliferation and migration to promote wound healing. However, these growth factors may have problems such as poor stability, high preparation costs and possible immune responses in practical applications. At the same time, the process of growth factor repairing the skin is relatively isolated and not effectively integrated with other functions. For example, a large number of free radicals generated during wound healing are not processed at the same time, which may affect the quality and speed of skin healing. For the antioxidant properties after skin damage, some studies have used natural antioxidants such as vitamin C, vitamin E and its derivatives, or some plant extracts to enhance the antioxidant activity of the skin. Although these antioxidants can neutralize free radicals to a certain extent, they lack a positive promoting effect on other physiological processes in the skin wound repair process, such as extracellular matrix reconstruction. Regarding the promotion of angiogenesis after skin damage, some studies have used substances such as vascular endothelial growth factor (VEGF) to promote angiogenesis, which mainly acts on vascular endothelial cells, stimulating the proliferation and migration of vascular endothelial cells, thereby promoting cardiovascular formation. However, when these substances are used alone to promote angiogenesis, their effects may be affected by changes in the local microenvironment, and the oxidative stress problem in the process of new blood vessel formation is not taken into account, nor is it effectively coordinated and integrated with the cell proliferation process in the skin wound repair process.

[0004] It can be seen that most of the substances with skin repair function in the prior art have a single function, and lack substances that can achieve multifunctional synergistic effects. Summary of the invention

[0005] The purpose of the present invention is to provide a repair polypeptide SC17-2 and its application in skin repair. The repair polypeptide SC17-2 has multiple functions of skin wound repair, improving skin antioxidant activity and promoting skin angiogenesis, thereby achieving a better skin repair function.

[0006] The present invention provides a repair polypeptide SC17-2, wherein the repair polypeptide SC17-2 comprises an amino acid sequence shown in SEQ ID NO:1.

[0007] Preferably, the two cysteines in the amino acid sequence shown in SEQ ID NO: 1 are linked to form a cyclic peptide via a disulfide bond.

[0008] The present invention also provides the use of the repair polypeptide SC17-2 described in the above technical solution in the preparation of skin repair products.

[0009] Preferably, the skin repair product includes a product having one or more functions of repairing skin wounds, improving skin antioxidant activity and promoting skin angiogenesis.

[0010] Preferably, the product comprises cosmetics and / or medicines.

[0011] Preferably, the medicine includes a medicine having one or more functions of treating body surface trauma, treating body surface burns and treating skin ulcers.

[0012] Preferably, the cosmetics include cosmetics having one or more functions of reducing scar formation, accelerating scar repair and anti-oxidation function.

[0013] The present invention also provides a skin repair product, wherein the active ingredient of the skin repair product includes the repair polypeptide SC17-2 described in the above technical solution.

[0014] Preferably, the product comprises cosmetics and / or medicines.

[0015] Preferably, the auxiliary materials in the cosmetics include one or more of sorbitol, butylene glycol, beeswax, mineral fat, tea tree oil, elastin, active peptides, herbal plant extracts, cell active ingredients and vitamins.

[0016] Beneficial effects:

[0017] The present invention provides a repair polypeptide SC17-2, wherein the repair polypeptide SC17-2 comprises an amino acid sequence shown in SEQ ID NO: 1. The repair polypeptide SC17-2 of the present invention has multiple functions of repairing skin wounds, improving skin antioxidant activity and promoting skin angiogenesis, thereby achieving a more excellent skin repair function.

[0018] The length of the amino acid sequence shown in SEQ ID NO:1 of the present invention is 17 amino acids, with 2 cysteines, which can form a cyclic peptide through a disulfide bond, thereby increasing the stability of the polypeptide; compared with the peptide chain length of the existing known skin repair polypeptides, the length of the repair polypeptide SC17-2 is relatively shorter, moderate in length, and low in production cost. At the same time, the proportion of hydrophobic amino acids in the repair polypeptide SC17-2 is 58.82%, which can take into account the characteristics of uniform dispersion in aqueous and lipidic substances. Furthermore, the isoelectric point of the repair polypeptide SC17-2 is 8.64, which can maintain good stability in acidic solvents, is conducive to the simplification of the preparation process, and is convenient for the preparation of more dosage forms of products. It can be seen that based on the above sequence characteristics and physicochemical properties, the repair polypeptide SC17-2 of the present invention has good chemical stability, strong adaptability to different environmental conditions and is easy to industrially synthesize. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0020] Figure 1 The toxicity test results of the repair polypeptide SC17-2 on HaCat cells in Example 2;

[0021] Figure 2 The UV damage repair activity test results of the repair polypeptide SC17-2 in Example 2;

[0022] Figure 3 The results of the cell migration promotion test of the repair polypeptide SC17-2 in Example 2, wherein A and B respectively represent the percentage of cell migration in the wound after adding VEGF and an equal volume of SC17-2 compared with the control group and the representative images in the cell scratch experiment;

[0023] Figure 4 The results of the skin repair activity test of the repair polypeptide SC17-2 in Example 2 in the animal model, wherein A, B and C represent the wound healing area images of the control group, VEGF and SC17-2 mice, respectively, the area change diagram of the representative images of the wound healing area of ​​mice in 11 days, and the change diagram of the wound area of ​​mice as the number of days changes;

[0024] Figure 5The effect of the repair polypeptide SC17-2 in Example 2 on angiogenesis, wherein the left figure shows the recovery of the intersegmental blood vessels in the trunk of zebrafish, wherein the control group was not treated in any way, and the right figure shows the images of the intersegmental blood vessels in the trunk under various treatments;

[0025] Figure 6 This is the effect of the repair polypeptide SC17-2 in Example 2 on the MAPK, TGF-β, and Smad2 / 3 signaling pathways. DETAILED DESCRIPTION

[0026] The present invention provides a repair polypeptide SC17-2, wherein the repair polypeptide SC17-2 comprises an amino acid sequence shown in SEQ ID NO:1.

[0027] The amino acid sequence shown in SEQ ID NO:1 of the present invention is specifically: SFMPLSIPIMCKMLSKC. As an embodiment, the cysteines at positions 11 and 17 of the amino acids shown in SEQ ID NO:1 form a pair of disulfide bonds to form a cyclic peptide, which can further increase the stability of the repair polypeptide SC17-2. The sequence of the repair polypeptide SC17-2 shown in SEQ ID NO:1 of the present invention is 17 amino acids, which is of moderate length, and the production cost is lower than that of overly long polypeptides.

[0028] The repair polypeptide SC17-2 of the present invention has an isoelectric point of 8.64. Compared with polypeptides with lower isoelectric points, it can maintain good stability in acidic solvents, which is conducive to process simplification during the preparation process and the preparation of more dosage forms of products.

[0029] The proportion of hydrophobic amino acids in the repair polypeptide SC17-2 of the present invention is 58.82%. Compared with polypeptides with higher hydrophobicity, it can be evenly dispersed in aqueous and lipid substances, ensuring the uniformity of the raw materials of the final product.

[0030] As an embodiment, the synthesis method of the repair polypeptide SC17-2 of the present invention is solid phase synthesis or recombinant expression. The present invention does not specifically limit the steps of the solid phase synthesis or recombinant expression, and the steps of conventional solid phase synthesis or recombinant expression in the art can be used; for example, the steps of the recombinant expression can be: constructing an expression plasmid; expressing the expression plasmid through a bacterial or fungal expression system, and purifying the obtained crude polypeptide to obtain the repair polypeptide SC17-2.

[0031] The present invention also provides the use of the repair polypeptide SC17-2 described in the above technical solution in the preparation of skin repair products. As an embodiment, the skin repair product of the present invention includes a product having one or more functions of skin wound repair, improving skin antioxidant activity and promoting skin angiogenesis; as another embodiment, the skin repair product of the present invention includes a product having the functions of skin wound repair, improving skin antioxidant activity and promoting skin angiogenesis. As an embodiment, the product of the present invention includes cosmetics and / or drugs; as another embodiment, the drug of the present invention includes a drug having one or more functions of treating body surface trauma, treating body surface burns and treating skin ulcers; as another embodiment, the cosmetics include cosmetics having one or more functions of reducing scar formation, accelerating scar repair and anti-oxidation function.

[0032] The present invention also provides a skin repair product, wherein the active ingredient of the skin repair product includes the repair polypeptide SC17-2 described in the above technical solution. As an embodiment, the product includes cosmetics and / or drugs. As another embodiment, the auxiliary materials in the cosmetics include one or more of sorbitol, butylene glycol, beeswax, mineral fat, tea tree oil, elastin, active peptides, herbal plant extracts, cell active ingredients and vitamins.

[0033] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0034] Example 1

[0035] The primary structure of the amino acid sequence of the repair polypeptide SC17-2 of the present invention is SFMPLSIPIMCKMLSKC (SEQ ID NO: 1), which is a small cyclic peptide, and the amino acids at positions 11 and 17 in the sequence are connected by a pair of disulfide bonds.

[0036] 2. The preparation method of repair peptide SC17-2 is as follows:

[0037] According to the designed amino acid sequence, a crude polypeptide was synthesized by solid phase synthesis, and the 11th and 17th cysteines were connected to form a pair of disulfide bonds. The connection condition was to react the crude polypeptide in hydrogen peroxide for 15 minutes.

[0038] The crude polypeptide is desalted and purified by HPLC reverse phase column chromatography, and its purity is identified until the purity of the polypeptide is not less than 95%;

[0039] The HPLC purification and identification method is as follows: 0.1 mg of the sample to be tested is dissolved in 1 mL of ultrapure water containing 0.1% trifluoroacetic acid. If there are insoluble impurities, filter with a 0.45 μm filter membrane; mobile phase A is an aqueous solution containing 0.1% trifluoroacetic acid, and mobile phase B is an acetonitrile solution containing 0.1% trifluoroacetic acid. Sample loading begins after the baseline is stable, and the sample loading volume is 50 μL; the chromatographic column is a silica gel alkyl bonded phase C18 column (4.6 mm×300 mm, colloid size 5 μm, pore size 100A), and a binary mobile phase gradient elution system is used to perform gradient elution, that is, within 30 minutes, the content of mobile phase B in the eluent increases linearly from 0% to 80%, the flow rate is 1 mL / min, the detection wavelength is 215 nm, and the measurement is performed at 25°C.

[0040] 3. Detection of the physical and chemical properties of repair peptide SC17-2

[0041] 1) The molecular weight of the repair polypeptide SC17-2 was determined to be 1925.95 Da by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF); the detection method was as follows: the purified repair polypeptide SC17-2 was dissolved in deionized water to prepare a 1 μmol / mL solution, 10 μL of the solution was taken and mixed with an equal volume of saturated matrix solution (α-cyano-4-hydroxycinnamic acid was dissolved in 50% acetonitrile solution containing 0.1% trifluoroacetic acid to prepare a saturated solution, centrifuged, and the supernatant was taken) and then measured.

[0042] 2) The isoelectric point of the purified repair polypeptide SC17-2 was determined to be 8.64 by isoelectric focusing electrophoresis. After reducing the disulfide bonds, the amino acid sequence structure of the purified polypeptide was determined by an automatic amino acid sequencer to be Ser-Phe-Met-Pro-Leu-Ser-Ile-Pro-Ile-Met-Cys-Lys-Met-Leu-Ser-Lys-Cys (SFMPLSIPI MCKMLSKC) to confirm the correctness of its amino acid sequence.

[0043] Example 2

[0044] Determination of the skin repair function of the repair polypeptide in Example 1

[0045] (I) Cytotoxicity assay

[0046] The toxicity of the repair polypeptide SC17-2 in Example 1 to human immortalized keratinocytes HaCat was detected by CCK8 method: Human immortalized keratinocytes HaCat were purchased from the Kunming Cell Bank of the Chinese Academy of Sciences. First, the adult immortalized keratinocytes HaCat were cultured in DMEM / F12 containing 15% fetal bovine serum and double antibodies (100 U / mL each of penicillin and streptomycin). After the cells were fully grown, they were digested with 0.25% trypsin, washed twice with the above culture medium, and the cells were resuspended. After the cells were counted, 100 μL of the cell suspension was added to a 96-well cell culture plate to make the number of cells in each well reach 10 5 indivual.

[0047] Eight experimental groups were set up: 20 μL of SC17-2 solution with concentrations of 100.00 μg / mL, 50.00 μg / mL, 25.00 μg / mL, 12.50 μg / mL, 6.25 μg / mL, 3.13 μg / mL, 1.56 μg / mL and 0.78 μg / mL were added to the cell culture plate, the same volume of sterilized ultrapure water was added to the control group (Control), and vascular endothelial cell factor (vegf) was added to the positive control group, and the amount added was 20 ng / mL. The eight experimental groups, the control group and the positive control group were cultured in a 37°C, 5% CO2 incubator for 24 hours. After the culture was completed, 10 μL of CCK8 solution was added to each well of the 96-well cell culture plate, and the culture was continued for 2-4 hours. The light absorption was detected by an enzyme marker, and the wavelength was 450 nm. Then the average of 3 biological replicates in the same treatment group was taken and divided by the average of 3 biological replicates in the control group, and the control group was taken as 100%. The results are as follows Figure 1 As shown in the figure, "*" and "**" indicate significant differences at P < 0.05 and P < 0.01, respectively.

[0048] Depend on Figure 1 It can be concluded that SC17-2, like VEGF, can promote the migration of HaCaT cells.

[0049] (II) Effect of promoting the repair of oxidative stress damage

[0050] HaCaT cells were cultured at 1×10 4Cells were inoculated in 24-well plates at 100 μg / well and incubated for 24 hours to ensure the uniformity and number of cells in each well. Before ultraviolet irradiation, the cells were washed with phosphate buffered saline (PBS) and covered with 300 mL of PBS. The blank control group was wrapped in tin foil to eliminate the effects of UV irradiation and then irradiated with 5 J / cm. Immediately after the irradiation, PBS was replaced with culture medium and repair peptide SC17-2 was added at a final concentration of 20 and 5 μg / mL, respectively, and incubated for 24 hours. After that, 10 μL of CCK-8 was added to each well and incubated for 2 hours. The absorbance of each well was measured at a wavelength of 450 nm, and then the average of 3 biological replicates in the same treatment group was taken and divided by the average of 3 biological replicates in the control group, with the control group as 100%.

[0051] The results are as follows Figure 2 As shown, "**" and "***" indicate significant differences of P < 0.01 and P < 0.001, respectively. It can be seen that the repair polypeptide SC17-2 has significant cell ultraviolet damage repair activity at a concentration of 20 μg / mL. From the above experiment, it can be seen that the repair polypeptide SC17-2 can repair the cell activity of HaCaT cells after UV irradiation, thereby achieving the effect of reducing ultraviolet damage.

[0052] (III) Repair peptide SC17-2 promotes cell migration activity.

[0053] The cell scratch test was used to measure the HaCat cells in the logarithmic growth phase. The cells were digested with 0.25% trypsin and made into a cell suspension in a culture medium containing 10% fetal bovine serum. 6 Cells were inoculated into 6-well plates at a density of 100 cells / well and cultured in a 37°C, 5% CO2 incubator until the cells completely filled the culture wells. The culture medium was aspirated and washed once with PBS. Use a sterile pipette tip to draw a line from top to bottom in the center of each test well perpendicular to the culture well. To ensure the same width, try to apply force evenly. Carefully wash away the culture medium and blow it with PBS several times to wash away the cell clusters produced by the scratches and make the scratch edges neat. After carefully aspirating PBS, add fresh serum-free culture medium and samples of appropriate concentrations, and take pictures under a microscope. Continue to culture in a cell culture incubator. During the culture process, observe every 6 hours and take pictures to record. To ensure that the same field of view is selected for each photo, mark the bottom of each culture well with a marker before inoculating cells. The obtained image data was analyzed and processed with Image Pro Plus 6.0. After evenly selecting 30 points on each side of the scratch edge, the midline represents the scratch edge, measure the scratch spacing, and calculate the scratch repair rate using the following formula: Scratch repair rate = (0h scratch width - 16h scratch width) / 0h scratch width. The experiment was repeated three times.

[0054] The cell scratch method was used to detect the effect of repair peptide SC17-2 at 20μg / mL and 5μg / mL concentrations on the migration activity of HaCat cells. Figure 3 As shown in the figure, "**" indicates significant difference P < 0.01. It can be seen that after 16 hours of SC17-2 action, there is a significant difference compared with the control group, indicating that SC17-2 has the effect of promoting keratinocyte migration.

[0055] (IV) Experiment on the wound healing effect of repair peptide SC17-2 on mouse skin injury model

[0056] Kunming mice aged 6-8 weeks (about 20g) were randomly selected and anesthetized with 1% sodium pentobarbital solution intraperitoneally (100μL / 20g), and the hair on their backs was cut as clean as possible with scissors. After disinfection with alcohol, a wound with a diameter of 8mm was made on the back with a hole puncher. Normal saline was used as the blank control group; mouse VEGF-A (mVEGF-A, 50μg / mL) was used as the positive control; repair polypeptide SC17-2 (20μg / mL) was used as the experimental group; VEGF-A and repair polypeptide SC17-2 were both prepared with normal saline solution. The drug was administered once a day, 20μL each time, and the reagents in the experimental group and the control group were added to the surface of the wound. The changes in the wound were photographed with a camera every two days until the medicated wound was completely scab-free and healed. When taking pictures, a ruler was used to compare the wound as a ruler. The results are as follows: Figure 4 As shown, in Figure 4 VEGF in the middle represents the positive control.

[0057] Depend on Figure 4 It can be concluded that in the 11-day wound observation, the repair peptide SC17-2 at a concentration of 20μg / ml has a significantly better effect on wound healing than the mouse VEGF-A at a concentration of 50μg / ml, which shows that it has significantly improved the wound repair ability. Therefore, the repair peptide SC17-2 can be used to prepare drugs for treating body surface trauma, burns, skin ulcers, reducing scar formation, and accelerating scar repair.

[0058] (V) Effects of skin repair peptides on angiogenesis in zebrafish

[0059] First, select healthy zebrafish embryos that have developed to 24 hours after fertilization, place them in clean water, and culture them to 24hpf at 28.5℃, with 14 hours of light and 10 hours of darkness alternating. Treat 24hpf embryos with 300ng / mL of VEGFR tyrosine kinase inhibitor II (VEGFRtyrosine kinase inhibitorII, VRI) for 3 hours. The treatment is carried out in a temperature-controlled and stable environment to ensure that VRI is evenly distributed. After treatment, the embryos are carefully transferred to a 24-well plate, with 12 per well. After incubation for 24 hours, slowly add the repair peptide SC17-2 to the 24-well plate in a gradient of 5μg / mL, 10μg / mL, and 20μg / mL. After adding the sample, shake it gently to mix, and pay attention to the strength. Then use a Leica fluorescence microscope to observe the morphological changes of the embryos, and adjust the microscope parameters to clearly present the vascular structure. The results are as follows Figure 5 As shown ( Figure 5 The scale bar in the middle right figure is 500 μm). The results showed that the repair peptide SC17-2 at a concentration of 20 μg / mL could significantly promote the formation of zebrafish blood vessels compared with the control.

[0060] (VI) Effect of repair peptide SC17-2 on MAPKs signaling pathway

[0061] HaCat cells were cultured in DMEM / F12 medium containing 10% fetal bovine serum until the logarithmic growth phase and, after trypsin digestion, were plated at a cell density of 1×10 6 / mL was inoculated into a 6-well cell culture plate. Cultured in a 37°C, 5% CO2 incubator for 4-6 hours. After the cells were completely attached, they were washed 2-3 times with PBS and replaced with serum-free medium for starvation treatment for 16 hours. 20μL of samples of different concentrations (10μg / mL or 20μg / mL) were added to each well, 20μL of sterile Milli-Q water was added to the blank control, and 20μL of VEGF was added as a positive control group. The samples were acted for 16 hours.

[0062] Aspirate the culture medium and wash the cells twice with 1 mL of pre-cooled PBS. Add 250 μL of RIPA cell lysis buffer [50 mM Tris-HCl (pH 7.4), 1% Nonidet P-40, 0.25% sodium deoxycholate, 150 mM NaCl, 1 mM EDTA, 1 mM PMSF, 1 mM NaF, 1 mM Na3VO4, 1 μg / mL of aprotinin, leupeptin and pepstatin] to each well of cells, lyse on ice for 30 min, centrifuge at 4°C, 12,000g for 20 min, carefully aspirate the supernatant, and dispense it into new eppendorf tubes, aspirate 2 μL to determine the protein concentration by Bradford method, and dispense the rest into certain volumes and quickly freeze at -20°C.

[0063] After mixing the cell lysate with 6×SDS sample buffer equal to 40 μg of protein, heat at 95-100°C for 5 min, cool and add to each lane. Constant voltage electrophoresis: 90V for stacking gel and 120V for separation gel. After electrophoresis, transfer to PVDF membrane by wet transfer. After transfer, wash with TBS for 5 min, and block with 5 mL blocking solution (1×TBS, 0.1% Tween-20, 5% skim milk powder) at room temperature for 1 h. Wash the membrane three times with TBST, 5 min each time. Then react with pre-diluted primary antibody (CST 4370TPhospho-p44 / 42MAPK(Erk1 / 2)(Thr202 / Tyr204)(D13.14.4E)XP, CST 4695T p44 / 42MAPK(Erk1 / 2)(137F5) purchased from Cell Signaling, USA) and place on a rolling shaker at 4°C overnight. Wash three times with TBST, 5 min each time. Then react with secondary antibody (Goat Anti-Rabbit IgG (H+L) HRP, purchased from Affinity Biosciences, OH. USA) pre-diluted with blocking buffer and shake gently for 1 hour. Wash three times with TBST, 5 min each time. Prepare ECL reaction substrate according to the manufacturer's instructions and expose to light in a dark room for color development. The results are shown in Figure 6 shown.

[0064] Figure 6 The results in the results showed that different concentrations of repair peptide SC17-2 activated the Smad2 / 3 pathway in a concentration-dependent manner. The increase in the p-MAPK / MAPK ratio in HaCat cells treated with repair peptide SC17-2 may indicate that the MAPK pathway is activated, and the activated MAPK pathway may promote cell activities during wound healing. TGF-β plays a key role in granulation tissue formation and scar formation by promoting fibroblast proliferation and collagen synthesis, thereby promoting wound filling and scar tissue formation. Smad protein is a downstream molecule of the TGF-β signaling pathway. The increase in Smad protein phosphorylation levels in HaCat cells after treatment with repair peptide SC17-2 and VEGF may reflect the increase in TGF-β signaling. The increase in TGF-β protein expression may be related to the fibrosis stage of wound healing.

[0065] From the above results, it can be concluded that the repair peptide SC17-2 can promote cell migration, recruitment of macrophages and fibroblasts, and angiogenesis, and act through the MAPK signaling pathway. Animal models show that the SC17-2 peptide has good wound repair function and has no hemolytic activity and cytotoxicity.

[0066] The repair polypeptide SC17-2 of the present invention is composed of 17 amino acids, is a cyclic peptide with a pair of disulfide bonds, and has a mature synthesis process, low synthesis cost, and high in vivo stability. Therefore, the repair polypeptide SC17-2 can effectively promote skin repair and be used in the preparation of drugs for treating body surface trauma, burns, skin ulcers, reducing scar formation, accelerating scar repair, and other fields of skin repair and regeneration beauty skin care products.

[0067] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A repair polypeptide SC17-2, characterized in that: The repair polypeptide SC17-2 includes the amino acid sequence shown in SEQ ID NO:

1.

2. The repair polypeptide SC17-2 according to claim 1, characterized in that The two cysteines in the amino acid sequence shown in SEQ ID NO: 1 are linked together by a disulfide bond to form a cyclic peptide.

3. Use of the repair polypeptide SC17-2 according to claim 1 or 2 in the preparation of skin repair products.

4. The use according to claim 3, characterized in that: The skin repair product includes a product having one or more functions of skin wound repair, improving skin antioxidant activity and promoting skin angiogenesis.

5. The use according to claim 3, characterized in that: The products include cosmetics and / or medicines.

6. The use according to claim 5, characterized in that: The medicine includes medicines having one or more functions of treating body surface trauma, treating body surface burns and treating skin ulcers.

7. The use according to claim 5, characterized in that: The cosmetics include cosmetics having one or more functions of reducing scar generation, accelerating scar repair and anti-oxidation.

8. A skin repair product, characterized in that: The active ingredient of the skin repair product includes the repair polypeptide SC17-2 according to claim 1 or 2.

9. The skin repair product according to claim 8, characterized in that The products include cosmetics and / or medicines.

10. The skin repair product according to claim 9, characterized in that The auxiliary materials in the cosmetics include one or more of sorbitol, butylene glycol, beeswax, mineral fat, tea tree oil, elastin, active peptides, herbal plant extracts, cell active ingredients and vitamins.

Images