Cell therapy devices

Abstract

The present application discloses a cell therapy device. The cell therapy device includes a main body and a cell therapy mechanism. The main body is provided with a first working chamber and a second working chamber that are independent of each other, and the first working chamber and the second working chamber have different working temperatures; the cell therapy mechanism includes a rotating shaft, a first container group and a second container group, the rotating shaft is rotatably arranged in the first working chamber and the second working chamber, the first container group is arranged in the first working chamber and is detachably mounted on the rotating shaft to at least perform cell activation, gene transduction, and cell amplification, and the second container group is arranged in the second working chamber and is detachably mounted on the rotating shaft to at least store cleaning fluid and culture medium. The cell therapy device in the present application has two different working chambers, and the two working chambers have different temperatures, which can meet the different temperature requirements of the CAR T cell therapy process.

Description

Technical Field

[0001] The present application relates to the technical field of medical equipment, and in particular, to a cell therapy device. Background Art

[0002] Recent successful clinical results from immunotherapy trials using chimeric antigen receptor (CAR) T cells have brought new hope to patients with previously incurable cancers. As these novel therapies progress from the clinical trial stage to commercial scale-up, challenges associated with cell manufacturing have emerged.

[0003] CAR T (Chimeric Antigen Receptor T-Cell immunotherapy) is a new type of targeted therapy for treating tumors. In recent years, it has achieved good results in clinical tumor treatment through optimization and improvement, especially in the treatment of malignant blood diseases. Therefore, CAR T cell therapy is a very promising new tumor immunotherapy method that is accurate, fast, efficient, and has the potential to cure cancer.

[0004] The CAR T cell therapy process mainly includes the extraction of patient blood samples, separation of T cells, cell activation, gene transduction, cell expansion, cell concentration, cell collection, automated cell processing, etc. At present, most of the devices used for CAR T cell therapy on the market are independent devices that perform the above steps. Some devices integrate two or three of the above steps, but the execution devices of each part are exposed to the air, which makes it difficult to meet the temperature requirements of the CAR T cell therapy process. Summary of the invention

[0005] The main purpose of the present application is to provide a cell therapy device, which has two different working chambers, and the two working chambers have different temperatures, which can meet the different temperature requirements of the CAR T cell therapy process.

[0006] According to one aspect of the present application, a cell therapy device is provided, the cell therapy device comprising:

[0007] A main body, wherein a first working chamber and a second working chamber are provided on the main body and are independent of each other, and the first working chamber and the second working chamber have different working temperatures;

[0008] A cell therapy mechanism, comprising a rotating shaft, a first container group and a second container group, wherein the rotating shaft is rotatably arranged in the first working chamber and the second working chamber, the first container group is arranged in the first working chamber and is detachably mounted on the rotating shaft to at least perform cell activation, gene transduction, and cell amplification, and the second container group is arranged in the second working chamber and is detachably mounted on the rotating shaft to at least store cleaning fluid and culture medium.

[0009] Furthermore, a first heating element, a first temperature detection element and a controller are provided on the main body. The first heating element is electrically connected to the controller for heating the first working chamber. The first temperature detection element is used to detect the temperature of the first working chamber and is connected to the controller. The controller controls the first heating element according to the signal transmitted by the first temperature detection element.

[0010] Furthermore, a second heating element, a second temperature detection element and a controller are provided on the main body. The second heating element is electrically connected to the controller for heating the second working chamber. The controller controls the second heating element according to a signal transmitted by the second temperature detection element.

[0011] Furthermore, the cell therapy mechanism further comprises a first fixing seat, the first fixing seat is located in the first working chamber and fixed on the rotating shaft, and a first accommodating cavity is provided on the first fixing seat;

[0012] The first container group includes a first container, a second container, a third container, a fourth container and a fifth container, the first container, the second container, the third container, the fourth container and the fifth container are arranged in sequence along the length direction of the first fixing seat, and are detachably mounted on the first fixing seat, the first container, the second container, the third container, the fourth container and the fifth container are all provided with a first connecting channel for communicating with the first accommodating cavity, and each of the first connecting channels is provided with a first control valve for controlling the on-off of the first connecting pipeline,

[0013] Among them, the first container is used for cell activation, the second container is used for filtering the activated cells, the third container is used for holding the virus solution for gene transduction of the cells, the fourth container is used for filtering the gene-transduced cells, and the fifth container is used for cell amplification.

[0014] Furthermore, the cell therapy mechanism further comprises a second fixing seat, the second fixing seat is located in the second working chamber and fixed on the rotating shaft, and a second accommodating cavity is provided on the second fixing seat;

[0015] The second container group includes a sixth container, a seventh container and an eighth container, the sixth container, the seventh container and the eighth container are arranged in sequence along the length direction of the second fixing seat and are detachably mounted on the second fixing seat, the sixth container, the seventh container and the eighth container are each provided with a second connecting channel for communicating with the second accommodating cavity, and each of the second connecting channels is provided with a second control valve for controlling the opening and closing of the second connecting channel;

[0016] The sixth container is used to store culture medium, the seventh container is used to wash cells, and the eighth container is used to store washing solution.

[0017] Further, the first accommodating cavity and the second accommodating cavity are communicated with each other through a connecting pipe, and the connecting pipe is arranged inside the rotating shaft or outside the rotating shaft.

[0018] Furthermore, the main body is further provided with a first mounting port and a second mounting port, the first mounting port is communicated with the first working chamber, and the second mounting port is communicated with the second working chamber;

[0019] a first cover plate, wherein the first cover plate is movably mounted on the main body to open or close the first mounting opening;

[0020] A second cover plate is movably mounted on the main body to open or close the second mounting opening.

[0021] Furthermore, a bracket is provided on one side of the first cover plate close to the first working cavity and on one side of the second cover plate close to the second working cavity, and the first cover plate and the second cover plate are both mounted on the main body through the bracket.

[0022] Furthermore, the main body is provided with an avoidance groove, and the avoidance groove extends along the height direction of the main body;

[0023] A slide rail assembly is arranged between the avoidance groove and the bracket, and a rotating part is arranged on the top of the avoidance groove. After the first cover plate or the second cover plate is driven by an external force to move the bracket along the slide rail assembly to the top of the avoidance groove, it can rotate along the rotating part so that the first cover plate or the second cover plate rests on the top of the main body.

[0024] Furthermore, the cell therapy device further comprises a support assembly, and the support assembly is used to support the stent when the stent moves to the top end of the main body.

[0025] Compared with the prior art, the technical solution of this application has at least the following technical effects:

[0026] The cell therapy device of the present application is adopted, because the main body is provided with a first working chamber and a second working chamber that are independent of each other, and the first working chamber and the second working chamber have different working temperatures. The temperature in the first working chamber can ensure the survival rate of the cells; the temperature in the second working chamber can ensure that the culture medium, cleaning fluid, etc. will not deteriorate. In this way, when the rotating shaft rotates to drive the first container group and the second container group to swing slowly for cell preparation, the first container group is arranged in the first working chamber to smoothly carry out at least cell activation, gene transduction, and cell expansion, and the second container group is arranged in the second working chamber to at least store cleaning fluid and culture medium, so as to provide culture medium to cells or clean cells during cell expansion. It can be seen that the present application can meet the different temperature requirements of the CAR T cell therapy process, has a simple structure, and is easy to implement. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0028] Figure 1 This is a schematic structural diagram of the cell therapy device disclosed in the embodiment of the present application at a first viewing angle;

[0029] Figure 2 It is a structural schematic diagram of the cell therapy device disclosed in the embodiment of the present application at a second viewing angle;

[0030] Figure 3 An exploded view of a cell therapy device disclosed in an embodiment of the present application;

[0031] Figure 4 It is a schematic structural diagram of the cell therapy device disclosed in the embodiment of the present application at a third viewing angle;

[0032] Figure 5 A side view schematic diagram of a cell therapy device disclosed in an embodiment of the present application;

[0033] Figure 6 for Figure 1 An exploded view of each container in the first perspective;

[0034] Figure 7 for Figure 1 An exploded view of each container in a second viewing angle;

[0035] Figure 8 It is a schematic diagram of the structures of the second container, the fourth container and the seventh container disclosed in this embodiment;

[0036] Fig. 9This is a schematic diagram of the workflow of the cell therapy device disclosed in the embodiments of the present application.

[0037] The above drawings include the following reference numerals:

[0038] 10. Main body; 101a. First working chamber; 101b. Second working chamber; 1021. First mounting port; 1022. Second mounting port; 103. Avoidance groove; 1031. Rotating part; 1032. Support block; 1033. Elastic element; 11A. First cover plate; 11B. Second cover plate; 111. First plate segment; 112. Second plate segment; 113. Bracket; 1131. First support beam; 11310. Track; 1132. Second support beam; 20. Cell therapy mechanism ; 21, rotating shaft; 221, first fixed seat; 222, second fixed seat; 23a, first container; 23b, second container; 23b1, first cavity; 23b2, second cavity; 23c, third container; 23d, fourth container; 23e, fifth container; 23f, sixth container; 23g, seventh container; 23h, eighth container; 233A, first connecting channel; 233B, second connecting channel; 2331, first channel; 2332, second channel; 236, filter baffle. DETAILED DESCRIPTION

[0039] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0040] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0041] Unless otherwise specifically stated, the relative arrangement, numerical expressions and numerical values ​​of the parts and steps set forth in these embodiments do not limit the scope of the present application. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the accompanying drawings are not drawn according to the actual proportional relationship. The technology, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be considered as a part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as being merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters represent similar items in the following drawings, and therefore, once a certain item is defined in an accompanying drawing, it does not need to be further discussed in subsequent drawings.

[0042] See also Figures 1 to 8 As shown, according to an embodiment of the present application, a cell therapy device is provided, which includes a main body 10 and a cell therapy mechanism 20.

[0043] Among them, the main body 10 is provided with a first working chamber 101a and a second working chamber 101b which are independent of each other, and the first working chamber 101a and the second working chamber 101b have different working temperatures; the cell therapy mechanism 20 includes a rotating shaft 21, a first container group and a second container group, the rotating shaft 21 is rotatably arranged in the first working chamber 101a and the second working chamber 101b, the first container group is arranged in the first working chamber 101a and is detachably mounted on the rotating shaft 21 for at least cell activation, gene transduction, and cell amplification, and the second container group is arranged in the second working chamber 101b and is detachably mounted on the rotating shaft 21 for at least storing cleaning fluid and culture medium.

[0044] In actual use, the first container group and the second container group can be slowly rotated in the first working chamber 101a and the second working chamber 101b to prepare cells. Taking the CAR T (Chimeric Antigen Receptor T-Cell Immunotherapy) chimeric antigen receptor T cell therapy process as an example, the CAR T cell therapy process mainly includes the processes of extracting patient blood samples, separating T cells, cell activation, gene transduction, cell expansion, cell concentration, cell collection, and automated cell processing. In cell activation, gene transduction, and cell expansion, the temperature is generally maintained at 37 degrees Celsius, which is close to the temperature of the human body and can ensure the survival rate of cell culture; in the process of cell expansion, it is generally necessary to provide cells with culture medium, etc., and the preservation of nutrients such as culture medium generally requires a lower temperature, such as about 4 degrees Celsius, which can ensure that the culture medium will not deteriorate.

[0045] In the present application, since the main body 10 is provided with a first working chamber 101a and a second working chamber 101b which are independent of each other, and the first working chamber 101a and the second working chamber 101b have different working temperatures. The temperature in the first working chamber 101a is generally maintained at about 37 degrees Celsius, which is close to the temperature of the human body and can ensure the survival rate of the cells; the temperature in the second working chamber 101b is generally maintained at about 4 degrees Celsius, which can ensure that the culture medium, cleaning fluid, etc. will not deteriorate. In this way, when the rotating shaft 21 rotates to drive the first container group and the second container group to swing slowly for cell preparation, the first container group is arranged in the first working chamber 101a, and at least cell activation, gene transduction, and cell expansion can be smoothly performed, and the second container group is arranged in the second working chamber 101b, at least for storing cleaning fluid and culture medium, so as to provide culture medium to cells or clean cells during cell expansion. Compared with the device for performing cell therapy exposed to air in the prior art, the present application can meet the different temperature requirements of the CAR T cell therapy process, has a simple structure, and is easy to implement.

[0046] Specifically, the main body 10 is provided with a first heating element (not shown in the figure), a first temperature detection element (not shown in the figure) and a controller (not shown in the figure), the first heating element is electrically connected to the controller for heating the first working chamber 101a, the first temperature detection element is used to detect the temperature of the first working chamber 101a and is connected to the controller, and the controller controls the first heating element according to the signal transmitted by the first temperature detection element. In other words, when the first temperature detection element detects that the temperature of the first working chamber 101a does not meet the requirements for cell activation, gene transduction, and cell amplification, at this time, the controller will control the first heating element to heat the first working chamber 101a, so that the temperature in the first working chamber 101a meets the requirements for cell activation, gene transduction, and cell amplification, without the need for manual detection, with a high degree of automation, and greatly improving work efficiency.

[0047] Further, the main body 10 is provided with a second heating element (not shown in the figure), a second temperature detection element (not shown in the figure) and a controller (not shown in the figure), the second heating element is electrically connected to the controller for heating the second working chamber 101b, and the controller controls the second heating element according to the signal transmitted by the second temperature detection element. In actual operation, when the second temperature detection element detects that the temperature of the second working chamber 101b is too low, the second temperature detection element transmits the temperature signal to the controller, and the controller controls the second heating element to generate heat to adjust the temperature in the second working chamber 101b. Optionally, since the temperature in the second working chamber 101b is relatively low, in order to keep the temperature of the second working chamber 101b at about 4 degrees Celsius, a heat dissipation device or a refrigeration device can also be provided in the second working chamber 101b in this embodiment, and the heat dissipation device or the refrigeration device is used to dissipate heat or cool the second working chamber 101b, so as to ensure that the temperature in the second working chamber 101b can store the culture medium and the cleaning solution, thereby ensuring that the culture medium and the cleaning solution will not deteriorate.

[0048] Optionally, the first heating element and the second heating element in this embodiment may be structures such as electric heating wires, electric heating plates, electric heating sheets, etc. The first temperature detection element and the second temperature detection element may be, for example, temperature sensors.

[0049] See also Figure 3 As shown, the cell therapy mechanism 20 further includes a first fixing seat 221 , which is located in the first working chamber 101 a and fixed on the rotating shaft 21 , and a first accommodating cavity (not shown in the figure) is provided on the first fixing seat 221 .

[0050] The first container group includes a first container 23a, a second container 23b, a third container 23c, a fourth container 23d and a fifth container 23e. The first container 23a, the second container 23b, the third container 23c, the fourth container 23d and the fifth container 23e are arranged in sequence along the length direction of the first fixing seat 221 and are detachably mounted on the first fixing seat 221. The first container 23a, the second container 23b, the third container 23c, the fourth container 23d and the fifth container 23e are all provided with a first connecting channel 233A for communicating with the first accommodating cavity, and each first connecting channel 233A is provided with a first control valve (not shown in the figure) for controlling the opening and closing of the first connecting channel 233A. Among them, the first container 23a is used for cell activation, the second container 23b is used for filtering the activated cells, the third container 23c is used for holding the virus solution to perform gene transduction on the cells, the fourth container 23d is used for filtering the cells after gene transduction, and the fifth container 23e is used for cell amplification.

[0051] See also Figure 3As shown, the cell therapy mechanism 20 in this embodiment further includes a second fixing seat 222, which is located in the second working chamber 101b and fixed on the rotating shaft 21, and a second accommodating cavity (not shown in the figure) is provided on the second fixing seat 222.

[0052] The second container group includes a sixth container 23f, a seventh container 23g and an eighth container 23h. The sixth container 23f, the seventh container 23g and the eighth container 23h are arranged in sequence along the length direction of the second fixed seat 222 and are detachably mounted on the second fixed seat 222. The sixth container 23f, the seventh container 23g and the eighth container 23h are all provided with a second connecting channel 233B for communicating with the second accommodating cavity, and each second connecting channel 233B is provided with a second control valve (not shown in the figure) for controlling the opening and closing of the second connecting channel 233B. Among them, the sixth container 23f is used to store culture medium, the seventh container 23g is used to wash cells, and the eighth container 23h is used to store washing liquid.

[0053] Combination Figure 1 , Figure 2 , Figure 3 , Figure 6 as well as Figure 7 As shown, when actually preparing cells, firstly, the first container 23a, the second container 23b, the third container 23c, the fourth container 23d and the fifth container 23e are mounted on the first fixing seat 221, so that the first connecting channels 233A on the first container 23a, the second container 23b, the third container 23c, the fourth container 23d and the fifth container 23e are all connected to the first accommodating cavity on the first fixing seat 221. The sixth container 23f, the seventh container 23g and the eighth container 23h are mounted on the second fixing seat 222, so that the second connecting channels 233B on the sixth container 23f, the seventh container 23g and the eighth container 23h are connected to the second accommodating cavity on the second fixing seat 222. At this time, the separated T cells are injected into the first container 23a, and the driving motor is used to drive the rotating shaft 21 to slowly swing from 0° to 270° to drive the first container group, the second container group and the first fixing seat 221 and the second fixing seat 222 to slowly rotate along with the rotating shaft 21.

[0054] Combination Figures 1 to 9As shown, after cell activation in the first container 23a, the first control valve of the first container 23a is controlled to open the first connecting channel 233A of the first container 23a, so that the activated cells can flow into the first accommodating cavity through the first container 23a; then the first control valve on the second container 23b is controlled to open the first connecting channel 233A on the second container 23b, so that the cell solution in the first accommodating cavity flows into the second container 23b for filtration, and then the filtered cell solution is released again into the first accommodating cavity on the first fixing seat 221. Then, the first control valve on the third container 23c is controlled by a controller or other structure to open the first connecting channel 233A on the first container 23a, so that the virus solution in the third container 23c flows into the first accommodating cavity on the first fixing seat 221 and mixes with the filtered cell solution and the culture medium for 24 hours to perform gene transduction. Thereafter, the fourth container 23d is controlled to open the first connecting channel 233A thereon, and the cell solution after gene transduction is released into the fourth container 23d. After the culture medium is filtered out in the fourth container 23d, it is released into the first containing cavity on the first fixing seat 221, and then released from the first containing cavity into the fifth container 23e for cell amplification.

[0055] Among them, the cells need to be expanded in the fifth container 23e for 7 days to 10 days. After the cells have expanded for a period of time, once it is detected that the culture medium in the fifth container 23e is insufficient, the second control valve in the sixth container 23f is controlled to open the second connecting channel 233B in the sixth container 23f. In this way, the culture medium in the sixth container 23f can flow into the fifth container 23e through the second accommodating cavity, thereby ensuring the nutrients for cell culture and thus ensuring cell expansion.

[0056] After the cells have been expanded to a certain extent, the cells that meet the requirements can be collected according to actual needs. For cells that do not meet the requirements, the seventh container 23g is controlled to open the second connecting channel 233B of the seventh container 23g, so that the cells that do not meet the requirements in the fifth container 23e can be released into the seventh container 23g. At the same time, the cleaning liquid in the eighth container 23h, for example, the sodium chloride solution, flows into the seventh container 23g through the second accommodating cavity, and the sodium chloride solution in the eighth container 23h can be used to clean and filter the cells in the seventh container 23g and then discharge them.

[0057] Specifically, see Figure 8As shown, the second container 23a, the fourth container 23a and the seventh container 23g are provided with a filter partition 236, and the filter partition 236 divides the internal chamber of the corresponding container into a first cavity 23b1 and a second cavity 23b2. That is to say, the first container 23a, the third container 23c, the fifth container 23e, the sixth container 26f and the eighth container 23h in this embodiment are not provided with a filter partition 236, while the second container 23a, the fourth container 23a and the seventh container 23g are provided with a filter partition 236. By providing the filter partition 236 in the second container 23a, the fourth container 23a and the seventh container 23g, some substances in the cells can be filtered to obtain a cell solution that meets the requirements.

[0058] The internal chamber of the corresponding container is divided into a first cavity 23b1 and a second cavity 23b2 by a filtering partition 236, so that the cells before and after filtration are respectively placed in the first cavity 23b1 and the second cavity 23b2. In order to facilitate the communication of the first cavity 23b1 and the second cavity 23b2 with the accommodating cavity on the fixing seat 22, the first connecting channel 233A on the second container 23a and the fourth container 23a and the second connecting channel 233B on the seventh container 23g include a first channel 2331 and a second channel 2332. Among them, the first channel 2331 is communicated with the first cavity 23b1, and the second channel 2332 is communicated with the second cavity 23b2, and the first channel 2331 and the second channel 2332 are both provided with the above-mentioned second control valve. In this way, the opening and closing of the first channel 2331 and the second channel 2332 can be controlled respectively.

[0059] In order to facilitate the transportation of substances, the first accommodating cavity and the second accommodating cavity in this embodiment are connected by a connecting pipe (not shown in the figure), and the connecting pipe is arranged inside the rotating shaft 21 or outside the rotating shaft 21. In this way, the culture medium in the sixth container 23f can flow from the second accommodating cavity to the first accommodating cavity through the connecting pipe, and then flow from the first accommodating cavity to the fifth container 23e, ensuring the nutrients for cell culture, thereby ensuring the expansion of cells. The connecting pipe can also transport the cells in the fifth container 23e that do not meet the requirements to the seventh container 23g, and the cells in the seventh container 23g can be filtered and washed with the sodium chloride solution in the eighth container 23h and then discharged.

[0060] Further, see Figure 4As shown, the main body 10 is further provided with a first installation port 1021 and a second installation port 1022, wherein the first installation port 1021 is communicated with the first working chamber 101a, and the second installation port 1022 is communicated with the second working chamber 101b. The first cover plate 11A is movably mounted on the main body 10 to open or close the first installation port 1021, and the second cover plate 11B is movably mounted on the main body 10 to open or close the second installation port 1022.

[0061] In this way, during the cell culture process, the first cover plate 11A is moved to close the first mounting port 1021, thereby sealing the first working chamber 101a; the second cover plate 11B is moved to close the second mounting port 1022, thereby sealing the second working chamber 101b, so as to better culture cells and preserve cleaning fluid and culture medium, while preventing external dust from entering the first working chamber 101a or the second working chamber 101b, so that the cell therapy device can stably perform its cell preparation actions.

[0062] Specifically, a bracket 113 is provided on one side of the first cover plate 11A close to the first working chamber 101 a and on one side of the second cover plate 11B close to the second working chamber 101 b , and the first cover plate 11A and the second cover plate 11B are both mounted on the main body 10 through the bracket 113 .

[0063] Furthermore, an avoidance groove 103 is provided on the main body 10, and the avoidance groove 103 extends along the height direction of the main body 10; a slide rail assembly is provided between the avoidance groove 103 and the bracket 113, and a rotating part 1031 is provided at the top of the avoidance groove 103. After the first cover plate 11A and / or the second cover plate 11B is driven by an external force to move the bracket 113 along the slide rail assembly to the top of the avoidance groove 103, it can rotate along the rotating part 1031 so that the cover plate rests on the top of the main body 10.

[0064] When the first mounting port 1021 or the second mounting port 1022 needs to be opened, the user applies an upward force to the first cover plate 11A or the second cover plate 11B. At this time, the bracket 113 can move upward along the slide rail assembly. When the bracket 113 moves to the top of the main body 10 along the slide rail assembly, the first cover plate 11A or the second cover plate 11B is pushed toward the back side of the main body 10 (away from the side of the first mounting port 1021 or the second mounting port 1022). At this time, the bracket 113 can rotate along the rotating portion 1031. When the bracket 113 rotates along the rotating portion 1031 toward the back side of the main body 10 and rests on the top of the main body 10, the first mounting port 1021 or the second mounting port can be fully opened.

[0065] When the first installation opening 1021 or the second installation opening 1022 needs to be closed, it is only necessary to apply force to the first cover plate 11A or the second cover plate 11B to make the first cover plate 11A or the second cover plate 11B rotate forward along the rotating portion 1031. When the bracket 113 on the first cover plate 11A or the second cover plate 11B can move downward along the slide rail assembly, the external force applied to the first cover plate 11A or the second cover plate 11B is released. The first cover plate 11A or the second cover plate 11B can drive the bracket 113 to move downward along the slide rail assembly under its own gravity to close the first installation opening 1021 or the second installation opening 1022. The structure is simple and easy to operate. Optionally, the rotating portion 1031 in this embodiment is a rotating shaft.

[0066] Optionally, the slide rail assembly in this embodiment includes a track 11310 and a slider (not shown in the figure) that cooperate with each other, one of the track 11310 and the slider is set on the bracket 113, and the other is set in the avoidance groove 103. That is to say, when the track 11310 is set on the bracket 113, the slider is set in the avoidance groove 103; when the track 11310 is set in the avoidance groove 103, the slider is set on the bracket 113. By setting the slide rail assembly as a track 11310 and a slider that cooperate with each other, when the bracket 113 moves in the avoidance groove 103, the slider can also limit the bracket 113 to prevent the bracket 113 from deflecting, and the bracket 113 can move smoothly, with a simple structure and convenient operation.

[0067] Further, the bracket 113 in this embodiment includes a first support beam 1131, which extends along the height direction of the first cover plate 11A or the second cover plate 11B, and the track 11310 is arranged on the first support beam 1131 and extends along the length direction of the first support beam 1131. In a specific embodiment of the present application, the track 11310 is arranged on the first support beam 1131, and the slider is arranged in the avoidance groove 103. When the first support beam 1131 is extended along the height direction of the first cover plate 11A or the second cover plate 11B, it is more convenient for the user to push or pull the bracket 113 to move.

[0068] Specifically, the first cover plate 11A and the second cover plate 11B both include a first plate segment 111 and a second plate segment 112, and the first plate segment 111 and the second plate segment 112 are enclosed to form an L-shaped structure, and the first support beam 1131 is arranged perpendicular to the first plate segment 111. By enclosing the first plate segment 111 and the second plate segment 112 to form an L-shaped structure, when the first cover plate 11A and the second cover plate 11B are in a closed state, the first cover plate 11A and the second cover plate 11B enclose the main body 10 to form a rectangular parallelepiped structure, so that the first working chamber 101a and the second working chamber 101b can be covered. Of course, in other embodiments of the present application, the first cover plate 11A or the second cover plate 11B can also be provided with a flat plate structure that can close the first mounting port 1021 or the second mounting port 1022. As long as it is other deformation methods under the conception of the present application, it is within the protection scope of the present application.

[0069] Furthermore, the bracket 113 also includes a second support beam 1132, which is connected between the second plate segment 112 and the first support beam 1131. By setting the second support beam 1132, the connection stability between the main body 10 and the first cover plate 11A or the second cover plate 11B is further strengthened; at the same time, it can also withstand the gravity of the first cover plate 11A or the second cover plate 11B, avoiding the situation of unstable installation due to only the first support beam 1131 supporting the first cover plate 11A or the second cover plate 11B, greatly improving the service life of the entire cell therapy device, with a simple structure and easy implementation. Optionally, the second support beam 1132 can be arranged perpendicular to the first support beam 1131 and the second plate segment 112, or can be arranged obliquely to the first support beam 1131 and the second plate segment 112.

[0070] See also Figure 5As shown, the track 11310 in this embodiment is a waist-shaped groove, that is, a long groove, which is arranged on the first support beam 1131, and the rotating part 1031 includes a rotating shaft, which is arranged at the top of the avoidance groove 103 and penetrates the waist-shaped groove. When the first mounting port 1021 or the second mounting port 1022 is opened, the first cover plate 11A or the second cover plate 11B and the bracket 113 are first pulled up. When the bottom end of the long rubbing groove moves to the rotating shaft, the first cover plate 11A or the second cover plate 11B is pushed toward the back of the main body 10. At this time, the bracket 113 can be rotated along the rotating shaft by a certain angle and then rest on the main body 10. The structure is simple and easy to use and operate. Optionally, there are multiple brackets 113 in the present application, and the multiple brackets 113 are arranged at intervals along the length direction of the main body 10, so as to stably support the first cover plate 11A or the second cover plate 11B. In a specific embodiment of the present application, there are two brackets 113 on the first cover plate 11A and the second cover plate 11B, and the two brackets 113 are respectively installed at both ends of the first cover plate 11A or the second cover plate 11B. In this way, the service life of the entire cell therapy device can be greatly improved while strengthening the installation stability between the first cover plate 11A and the second cover plate 11B and the main body 10.

[0071] Of course, in other embodiments of the present application, the brackets 113 can also be set to three or more. As long as they are other deformation methods based on the concept of the present application, they are within the protection scope of the present application.

[0072] Furthermore, in this embodiment, the first cover plate 11A and the second cover plate 11B are both transparent cover plates. Setting the first cover plate 11A or the second cover plate 11B as a transparent cover makes it more convenient for users or patients to observe changes in cells in the cell therapy device when culturing or processing cells in each container.

[0073] Furthermore, the cell therapy device further includes a support assembly, which is used to support the support 113 when the support 113 moves to the top of the main body 10. After the cell culture is completed, when each container needs to be removed from the cell therapy device and thrown away, the support assembly is provided to effectively prevent the support 113 from sliding down when it moves to the top of the main body 10 and closing the first installation port 1021 or the second installation port 1022.

[0074] Specifically, see again Figure 5 As shown, the support assembly in this embodiment includes a support block 1032 and an elastic element 1033 . The support block 1032 is disposed in the side wall of the avoidance groove 103 , and the elastic element 1033 is pressed between the support block 1032 and the side wall of the avoidance groove 103 .

[0075] In actual use, when the first cover plate 11A or the second cover plate 11B drives the bracket 113 to move along the slide rail assembly to the top of the avoidance groove 103 under the action of external force, the bracket 113 stops at the outside of the support block 1032, and the support block 1032 compresses the elastic element 1033; when the first cover plate 11A or the second cover plate 11B moves to the top of the main body 10, the bracket 113 avoids the support block 1032, and the support block 1032 can extend into the avoidance groove 103 under the support of the elastic element 1033, and then support at the bottom of the bracket 113, preventing the first cover plate 11A or the second cover plate 11B from sliding downward along the slide rail assembly, and the structure is simple and easy to implement. Optionally, the support block 1032 in this embodiment is a wedge block, and the elastic element 1033 can be provided with a spring or a spring sheet or other structures.

[0076] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figure. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figure. For example, if the device in the accompanying drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below other devices or structures". Thus, the exemplary term "above" can include both "above" and "below". The device can also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.

[0077] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of this application.

[0078] The above are only preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A cell therapy device, characterized in that: include: A main body (10), wherein the main body (10) is provided with a first working chamber (101a) and a second working chamber (101b) that are independent of each other, and the first working chamber (101a) and the second working chamber (101b) have different working temperatures; A cell therapy mechanism (20), the cell therapy mechanism (20) comprising a rotating shaft (21), a first container group and a second container group, the rotating shaft (21) being rotatably arranged in the first working chamber (101a) and the second working chamber (101b), the first container group being arranged in the first working chamber (101a) and being detachably mounted on the rotating shaft (21) to at least perform cell activation, gene transduction, and cell amplification, the second container group being arranged in the second working chamber (101b) and being detachably mounted on the rotating shaft (21) to at least store cleaning fluid and culture medium.

2. The cell therapy device according to claim 1, characterized in that: The main body (10) is provided with a first heating element, a first temperature detection element and a controller; the first heating element is electrically connected to the controller for heating the first working chamber (101a); the first temperature detection element is used to detect the temperature of the first working chamber (101a) and is connected to the controller; the controller controls the first heating element according to a signal transmitted by the first temperature detection element.

3. The cell therapy device according to claim 1, characterized in that: The main body (10) is provided with a second heating element, a second temperature detection element and a controller; the second heating element is electrically connected to the controller for heating the second working chamber (101b); the controller controls the second heating element according to a signal transmitted by the second temperature detection element.

4. The cell therapy device according to claim 1, characterized in that: The cell therapy mechanism (20) further comprises a first fixing seat (221), wherein the first fixing seat (221) is located in the first working chamber (101a) and is fixed on the rotating shaft (21), and a first accommodating cavity is provided on the first fixing seat (221); The first container group comprises a first container (23a), a second container (23b), a third container (23c), a fourth container (23d) and a fifth container (23e); the first container (23a), the second container (23b), the third container (23c), the fourth container (23d) and the fifth container (23e) are arranged in sequence along the length direction of the first fixing seat (221), and are detachably mounted on the first fixing seat (221); the first container (23a), the second container (23b), the third container (23c), the fourth container (23d) and the fifth container (23e) are each provided with a first connecting channel (233A) for communicating with the first accommodating cavity, and each of the first connecting channels (233A) is provided with a first control valve for controlling the opening and closing of the first connecting channel (233A); The first container (23a) is used for cell activation, the second container (23b) is used for filtering the activated cells, the third container (23c) is used for holding a virus solution for gene transduction of cells, the fourth container (23d) is used for filtering the gene-transduced cells, and the fifth container (23e) is used for cell amplification.

5. The cell therapy device according to claim 4, characterized in that: The cell therapy mechanism (20) further comprises a second fixing seat (222), wherein the second fixing seat (222) is located in the second working chamber (101b) and is fixed on the rotating shaft (21), and a second accommodating cavity is provided on the second fixing seat (222); The second container group comprises a sixth container (23f), a seventh container (23g) and an eighth container (23h); the sixth container (23f), the seventh container (23g) and the eighth container (23h) are arranged in sequence along the length direction of the second fixing seat (222) and are detachably mounted on the second fixing seat (222); the sixth container (23f), the seventh container (23g) and the eighth container (23h) are each provided with a second connecting channel (233B) for communicating with the second accommodating cavity; each second connecting channel (233B) is provided with a second control valve for controlling the opening and closing of the second connecting channel (233B); The sixth container (23f) is used to store culture medium, the seventh container (23g) is used to wash cells, and the eighth container (23h) is used to store washing solution.

6. The cell therapy device according to claim 5, characterized in that: The first accommodating cavity and the second accommodating cavity are connected via a connecting pipe, and the connecting pipe is arranged inside the rotating shaft (21) or outside the rotating shaft (21).

7. The cell therapy device according to claim 1, characterized in that: The main body (10) is further provided with a first installation opening (1021) and a second installation opening (1022), wherein the first installation opening (1021) is communicated with the first working chamber (101a), and the second installation opening (1022) is communicated with the second working chamber (101b); a first cover plate (11A), the first cover plate (11A) being movably mounted on the main body (10) to open or close the first mounting opening (1021); A second cover plate (11B), the second cover plate (11B) is movably mounted on the main body (10) to open or close the second mounting opening (1022).

8. The cell therapy device according to claim 7, characterized in that: A bracket (113) is provided on one side of the first cover plate (11A) close to the first working chamber (101a), and on one side of the second cover plate (11B) close to the second working chamber (101b); the first cover plate (11A) and the second cover plate (11B) are both mounted on the main body (10) via the bracket (113).

9. The cell therapy device according to claim 8, characterized in that: The main body (10) is provided with an avoidance groove (103), and the avoidance groove (103) extends along the height direction of the main body (10); A slide rail assembly is provided between the avoidance groove (103) and the bracket (113); a rotating portion (1031) is provided at the top of the avoidance groove (103); after the first cover plate (11A) or the second cover plate (11B) is driven by an external force to move the bracket (113) along the slide rail assembly to the top of the avoidance groove (103), the first cover plate (11A) or the second cover plate (11B) can rotate along the rotating portion (1031) so that the first cover plate (11A) or the second cover plate (11B) rests on the top of the main body (10).

10. The cell therapy device according to claim 8, characterized in that: The cell therapy device further comprises a support assembly, wherein the support assembly is used to support the support (113) when the support (113) moves to the top end of the main body (10).

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