Cell therapy devices

Abstract

The present application discloses a cell therapy device, including a main body, a cell therapy mechanism and a cell collection mechanism. A working chamber, a cell outlet pipe and a collection chamber are provided on the main body; the cell therapy mechanism includes a driving member, a rotating shaft and a container assembly, the rotating shaft is rotatably arranged in the working chamber, the driving member is arranged on the main body and is connected to the rotating shaft to drive the rotating shaft to swing in the working chamber, and the container assembly is detachably fixed on the rotating shaft to at least perform cell activation, gene transduction, cell amplification and cell cleaning; the cell collection mechanism is arranged on the main body, one end of the cell outlet pipe is connected to the container assembly, and the other end of the cell outlet pipe is connected to the collection chamber and extends into the collection chamber to connect with the cell collection mechanism. The present application can integrate the processes of cell activation, gene transduction, cell amplification, cell cleaning, cell collection, etc. in the preparation process of CAR T cells on the cell therapy device, with higher automation and cell preparation efficiency.

Description

Technical Field

[0001] The present application relates to the technical field of cell therapy equipment, and in particular, to a cell therapy device. Background Art

[0002] Recent successful clinical results from immunotherapy trials using chimeric antigen receptor (CAR) T cells have brought new hope to patients with previously incurable cancers. As these novel therapies progress from the clinical trial stage to commercial scale-up, challenges associated with cell manufacturing have emerged.

[0003] CAR T (Chimeric Antigen Receptor T-Cell immunotherapy) is a new type of targeted therapy for treating tumors. In recent years, it has achieved good results in clinical tumor treatment through optimization and improvement, especially in the treatment of malignant blood diseases. Therefore, CAR T cell therapy is a very promising new tumor immunotherapy method that is accurate, fast, efficient, and has the potential to cure cancer.

[0004] The CAR T cell therapy process mainly includes the extraction of patient blood samples, separation of T cells, cell activation, gene transduction, cell expansion, cell concentration, cell collection, automated cell processing, etc. At present, most of the devices used for CAR T cell therapy on the market are independent devices that perform the above steps. There is no device that can integrate multiple or all of the above steps on one instrument, and all of them use manual methods to perform cell activation, gene transduction, cell expansion, cell concentration, cell collection, etc., with low automation, high labor intensity and low efficiency in cell preparation. Summary of the invention

[0005] The main purpose of the present application is to provide a cell therapy device that can integrate processes such as cell activation, gene transduction, cell expansion, cell cleaning, and cell collection in the CAR T cell preparation process, with a high degree of automation and high cell preparation efficiency.

[0006] According to one aspect of the present application, a cell therapy device is provided, comprising:

[0007] A main body, wherein the main body is provided with a working chamber, a cell outlet pipe and a collection chamber;

[0008] A cell therapy mechanism, comprising a driving member, a rotating shaft and a container assembly, wherein the rotating shaft is rotatably arranged in the working chamber, the driving member is arranged on the main body and is drivingly connected to the rotating shaft to drive the rotating shaft to swing in the working chamber, and the container assembly is detachably fixed on the rotating shaft to at least perform cell activation, gene transduction, cell amplification and cell washing;

[0009] A cell collection mechanism is arranged on the main body, one end of the cell outlet pipe is connected to the container assembly, and the other end of the cell outlet pipe is communicated with the collection bin and extends into the collection bin to be connected to the cell collection mechanism.

[0010] Furthermore, a fixing seat is fixedly mounted on the rotating shaft, and a receiving cavity is arranged in the fixing seat;

[0011] The container assembly includes a plurality of containers, which are arranged in sequence along the length direction of the rotating shaft, and each of the containers is provided with a connecting channel for communicating with the accommodating cavity, and the connecting channel is provided with a control valve for controlling the opening and closing of the connecting channel.

[0012] Further, the fixing seat includes a first fixing seat, the first fixing seat is provided with a first accommodating cavity, the container assembly includes a first container, a second container, a third container, a fourth container and a fifth container arranged in sequence along the length direction of the rotating shaft, and the first container, the second container, the third container, the fourth container and the fifth container are all provided with the connecting channel communicating with the first accommodating cavity, wherein:

[0013] The first container is used for cell activation, and a magnetic attraction structure is provided on the main body, and the magnetic attraction structure is located above the first container;

[0014] The second container is used to filter the activated cells;

[0015] The third container is used to store the virus solution;

[0016] The fourth container is used to filter the cell solution after gene transduction;

[0017] The fifth container is used to expand the cells after filtration.

[0018] Further, the fixing seat includes a second fixing seat, and the second fixing seat is provided with a second accommodating cavity. The container assembly also includes a sixth container, a seventh container and an eighth container arranged in sequence along the length direction of the rotating shaft, and the sixth container, the seventh container and the eighth container are all located on the side of the fifth container away from the fourth container, and the sixth container, the seventh container and the eighth container are all provided with the connecting channel communicating with the second accommodating cavity, wherein,

[0019] The sixth container is used to store culture medium;

[0020] The seventh container is used to wash the cell solution;

[0021] The eighth container is used to store cleaning liquid.

[0022] Furthermore, the cell therapy device further comprises a connecting pipe, wherein the connecting pipe is used to connect the first accommodating cavity and the second accommodating cavity, and the connecting pipe is embedded in the rotating shaft or arranged outside the rotating shaft.

[0023] Further, one end of the cell outlet pipe is connected to the fifth container, the other end of the cell outlet pipe is connected to the collection chamber and extends into the collection chamber, the cell outlet pipe is provided with an outlet, and the outlet is provided with an outlet switch;

[0024] The cell collection mechanism comprises:

[0025] A collecting rack, the collecting rack being slidably mounted on the collecting bin, and the collecting rack having a first position of sliding into the collecting bin and a second position of sliding out of the collecting bin;

[0026] A collecting bag is detachably mounted on the collecting rack, and when the collecting rack is in the first position, the inlet of the collecting bag is aligned with the liquid outlet.

[0027] Furthermore, the collecting bag includes a bag body and a connecting tube connected to the bag body, the collecting bag is detachably mounted on the collecting rack, the connecting tube protrudes from the collecting rack, and the connecting tube opens the liquid outlet switch when the collecting bin is in the first position so that the liquid in the cell outlet pipe flows into the bag body.

[0028] Furthermore, the collecting bag is detachably mounted on the collecting rack, the connecting tube protrudes from the collecting rack, and the connecting tube opens the liquid outlet switch when the collecting bin is in the first position so that the liquid in the cell liquid outlet pipe flows into the bag body.

[0029] Furthermore, a detection element is provided in the collection bin, the cell therapy device includes a controller, the detection element is electrically connected to the controller, the detection element is used to detect whether the collection bag is placed on the collection rack when the collection rack is in the first position, and the controller controls the liquid outlet switch according to the signal transmitted by the detection element.

[0030] Furthermore, the cell outlet pipe is provided with a plurality of the outlets, the collecting racks are multiple, the multiple collecting racks are arranged side by side in the collecting bin, and the multiple collecting racks are arranged one-to-one with the multiple outlets.

[0031] Furthermore, the driving member is a driving motor, and the driving motor is drivingly connected to the rotating shaft to drive the rotating shaft to rotate within a range of 0° to 270°.

[0032] Compared with the prior art, the technical solution of this application has at least the following technical effects:

[0033] In the present application, a working chamber is provided on the main body, and a rotating shaft is rotatably arranged in the working chamber. When the cells are actually prepared, the container assembly is fixed on the rotating shaft. Since a driving member is provided on the main body, and the driving member is connected to the rotating shaft, the rotating shaft can be driven to swing slowly, so as to drive the container assembly to swing slowly with the rotating shaft. During the swinging process of the container assembly, the solution inside can be effectively mixed. Specifically, the container assembly can at least perform processes such as cell activation, gene transduction, cell amplification, and cell washing, and then the preparation of CAR T cells can be completed. The prepared cell solution can be transported to the collection bin through the cell outlet pipe for collection by the cell collection mechanism. Compared with the method of manually completing the processes of cell activation, gene transduction, cell amplification, cell washing, and cell collection on each independent device in the prior art, the cell therapy device in the present application uses a driving member to drive the rotating shaft to rotate to drive the container assembly to swing slowly with the rotating shaft, and integrate the processes such as cell activation, gene transduction, cell amplification, and cell washing on one instrument, which greatly improves the efficiency of cell preparation, has low labor intensity, and a higher degree of automation. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0035] Figure 1 This is a schematic diagram of the structure of the cell therapy device (without the cover plate) disclosed in the embodiment of the present application;

[0036] Figure 2 for Figure 1The main view of

[0037] Figure 3 An exploded view of a cell therapy device disclosed in an embodiment of the present application;

[0038] Figure 4 It is a schematic diagram of the structure of the first container, the third container, the fifth container, the sixth container, and the eighth container disclosed in the embodiments of the present application;

[0039] Figure 5 It is a schematic diagram of the structures of the second container, the fourth container and the seventh container disclosed in the embodiments of the present application;

[0040] Figure 6 for Figure 1 An exploded view of each cell container in the figure when it is in the first perspective;

[0041] Figure 7 for Figure 1 An exploded view of each cell container in the figure when it is at a second viewing angle;

[0042] Figure 8 for Figure 1 A partial schematic diagram of a collection rack portion of a cell therapy device disclosed in;

[0043] Fig. 9 This is a schematic diagram of the structure of the collection rack and the collection bag disclosed in the embodiment of the present application;

[0044] Fig.10 This is a schematic diagram of the structure of the collection bin disclosed in the embodiment of the present application when it is at a first viewing angle;

[0045] Fig.11 It is a schematic diagram of the structure of the collection bin disclosed in the embodiment of the present application when it is at a second viewing angle;

[0046] Fig.12 for Figure 1 A schematic diagram of the local structure of the cell therapy device disclosed in;

[0047] Fig.13 A partial view of a cell therapy device disclosed in an embodiment of the present application;

[0048] Fig.14 A schematic diagram of the structure of the cell therapy device disclosed in an embodiment of the present application at a certain viewing angle;

[0049] Fig.15 This is a schematic diagram of the structure of the cell therapy device disclosed in the embodiment of the present application when it is in another viewing angle;

[0050] Fig.16 A side view schematic diagram of a cell therapy device disclosed in an embodiment of the present application;

[0051] Fig.17 This is a schematic diagram of the working process of the cell therapy device disclosed in the embodiment of the present application;

[0052] Fig.18 This is a flow chart of a control method of a cell therapy device disclosed in an embodiment of the present application.

[0053] The above drawings include the following reference numerals:

[0054] 10. Main body; 101. Working chamber; 101a. First working chamber; 101b. Second working chamber; 102. Mounting port; 1021. First mounting port; 1022. Second mounting port; 103. Avoidance slot; 1031. Rotating part; 1032. Support block; 1033. Elastic element; 11. Cover plate; 11A. First cover plate; 11B. Second cover plate; 111. First plate section; 112. Second plate section; 113. Bracket; 1131. First support beam; 11310. Track; 1132. Second support beam; 12. Cell outlet pipe; 121. Outlet; 1211. Outlet switch; 13. Collection chamber; 131. Slide; 132. Limiting block; 141. Photographing element; 142. Mounting bracket; 15. Slide rail; 16. Magnetic structure; 20. Cell therapy mechanism; 21. Rotating shaft; 22, fixing seat; 221, first fixing seat; 222, second fixing seat; 23, container; 230, semicircular body; 2301, first plane; 231, accommodating cavity; 232, opening; 233, connecting channel; 2331, first channel; 2332, second channel; 234, cover body; 235, avoidance groove; 236, filter partition; 237, collection channel; 23a, first container; 23b, second container; 23b1, first cavity; 23b2, second cavity; 23c, third container; 23d, fourth container; 23e, fifth container; 23f, sixth container; 23g, seventh container; 23h, eighth container; 31, cam; 40, collecting rack; 41, avoidance space; 42, positioning hole; 43, handle; 50, collecting bag; 51, bag body; 52, connecting pipe. DETAILED DESCRIPTION

[0055] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0056] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0057] Unless otherwise specifically stated, the relative arrangement, numerical expressions and numerical values ​​of the parts and steps set forth in these embodiments do not limit the scope of the present application. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the accompanying drawings are not drawn according to the actual proportional relationship. The technology, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be considered as a part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as being merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters represent similar items in the following drawings, and therefore, once a certain item is defined in an accompanying drawing, it does not need to be further discussed in subsequent drawings.

[0058] See also Figure 1 to Figure 2 As shown, according to an embodiment of the present application, a cell therapy device is provided, which includes a main body 10, a cell therapy mechanism 20 and a cell collection mechanism.

[0059] Among them, a working chamber 101 is arranged on the main body 10; the cell therapy mechanism 20 includes a driving member (not shown in the figure), a rotating shaft 21 and a container assembly, the rotating shaft 21 is rotatably arranged in the working chamber 101, the driving member is arranged on the main body 10 and is drivingly connected to the rotating shaft 21 to drive the rotating shaft 21 to swing in the working chamber 101, and the container assembly is detachably fixed on the rotating shaft 21 to at least perform cell activation, gene transduction, cell amplification and cell cleaning; the cell collection mechanism is arranged on the main body 10, one end of the cell outlet pipe 12 is connected to the container assembly, and the other end of the cell outlet pipe 12 is communicated with the collection chamber 13 and extends into the collection chamber 13 to be connected with the cell collection mechanism, so that the cell collection mechanism can collect the cell solution.

[0060] In the present application, a working chamber 101 is provided on the main body 10, and a rotating shaft 21 is rotatably provided in the working chamber 101. When actually preparing cells, the container assembly is fixed on the rotating shaft 21. Since a driving member is provided on the main body 10, and the driving member is connected to the rotating shaft 21, the rotating shaft 21 can be driven to swing slowly, so as to drive the container assembly to swing slowly with the rotating shaft 21. During the swinging process of the container assembly, the solution inside it can be effectively mixed. Specifically, the container assembly can at least perform processes such as cell activation, gene transduction, cell amplification, and cell washing, and then the preparation of CAR T cells can be completed. The prepared cell solution can be transported to the collection bin 13 through the cell outlet pipe 12 for collection by the cell collection mechanism. Compared with the prior art method of manually completing processes such as cell activation, gene transduction, cell amplification and cell cleaning on each independent device, the cell therapy device in this embodiment uses a driving member to drive the rotating shaft 21 to rotate so as to drive the container assembly to slowly swing along with the rotating shaft 21, thereby integrating processes such as cell activation, gene transduction, cell amplification and cell cleaning on one instrument, thereby greatly improving the efficiency of cell preparation, reducing manual labor intensity and achieving a higher degree of automation.

[0061] See again Figure 1 As shown, the working chamber 101 in this embodiment includes a first working chamber 101a and a second working chamber 101b that are independent of each other, and the first working chamber 101a and the second working chamber 101b have different working temperatures. The container group includes a first container group and a second container group, the first container group is arranged in the first working chamber 101a to at least perform cell activation, gene transduction, and cell amplification, and the second container group is arranged in the second working chamber 101b to at least store cleaning solution and culture medium.

[0062] In the actual cell culture process, the temperature in the first working chamber 101a is generally maintained at about 37 degrees Celsius, which is close to the temperature of the human body and can ensure the survival rate of the cells. The temperature in the second working chamber 101b is generally maintained at about 4 degrees Celsius, which can ensure that the culture medium, cleaning fluid, etc. will not deteriorate. In this way, when the rotating shaft 21 rotates to drive the first container group and the second container group to swing slowly for cell preparation, the first container group is set in the first working chamber 101a to smoothly perform at least cell activation, gene transduction, and cell expansion, and the second container group is set in the second working chamber 101b to at least store cleaning fluid and culture medium, so as to provide culture medium to cells or clean cells during cell expansion. In this way, the different temperature requirements of the CAR T cell therapy process can be met, the structure is simple, and it is easy to implement.

[0063] Furthermore, the main body 10 is provided with a first heating element (not shown in the figure), a first temperature detection element (not shown in the figure) and a controller (not shown in the figure), the first heating element is electrically connected to the controller for heating the first working chamber 101a, the first temperature detection element is used to detect the temperature of the first working chamber 101a and is connected to the controller, and the controller controls the first heating element according to the signal transmitted by the first temperature detection element. In other words, when the first temperature detection element detects that the temperature of the first working chamber 101a does not meet the requirements for cell activation, gene transduction, and cell amplification, at this time, the controller will control the first heating element to heat the first working chamber 101a, so that the temperature in the first working chamber 101a meets the requirements for cell activation, gene transduction, and cell amplification. No manual detection is required, the degree of automation is high, and the work efficiency is greatly improved.

[0064] Further, the main body 10 is provided with a second heating element (not shown in the figure), a second temperature detection element (not shown in the figure) and a controller (not shown in the figure), the second heating element is electrically connected to the controller for heating the second working chamber 101b, and the controller controls the second heating element according to the signal transmitted by the second temperature detection element. In actual operation, when the second temperature detection element detects that the second working chamber 101b is too low, the second temperature detection element transmits the temperature signal to the controller, and the controller controls the second heating element to generate heat to adjust the temperature in the second working chamber 101b. Optionally, since the work required for the second working chamber 101b is relatively low, in order to keep the temperature of the second working chamber 101b at about 4 degrees Celsius, a heat dissipation device or a refrigeration device can also be provided in the second working chamber 101b in this embodiment, and the heat dissipation device or the refrigeration device is used to dissipate heat or cool the second working chamber 101b, so as to ensure that the temperature in the second working chamber 101b can store the culture medium and the cleaning solution, thereby preventing the culture medium and the cleaning solution from deteriorating.

[0065] Optionally, the first heating element and the second heating element in this embodiment may be structures such as electric heating wires, electric heating plates, electric heating sheets, etc. The first temperature detection element and the second temperature detection element may be, for example, temperature sensors.

[0066] Further, see Figure 3 As shown, a fixing seat 22 is fixedly mounted on the rotating shaft 21, and a receiving cavity (not shown in the figure) is arranged in the fixing seat 22.

[0067] The container assembly includes a plurality of containers 23, which are arranged in sequence along the length direction of the rotating shaft 21, and each container 23 is provided with a connecting channel 233 for communicating with the accommodating cavity, and a control valve (not shown in the figure) for controlling the on-off of the connecting channel 233 is provided on the connecting channel 233. In this way, the on-off of the connecting channel 233 of each container 23 can be controlled by controlling the control valve of each container 23, so that the cells in the container 23 flow into the accommodating cavity, and then flow from the accommodating cavity into other containers 23, so as to perform gene transduction, cell amplification, cell washing and other processes, and thus the preparation of CAR T cells can be completed.

[0068] Specifically, the fixing seat 22 includes a first fixing seat 221 and a second fixing seat 222. The first fixing seat 221 is arranged in the first working chamber 101a and fixed on the rotating shaft 21, and a first accommodating cavity is arranged in the first fixing seat 221; the second fixing seat 222 is arranged in the second working chamber 101b and fixed on the rotating shaft 21, and a second accommodating cavity is arranged in the second fixing seat 222. The container assembly includes 8 independent containers, specifically, the 8 containers are arranged in sequence along the length direction of the rotating shaft 21, and are divided into a first container group and a second container group. The first container group includes a first container 23a, a second container 23b, a third container 23c, a fourth container 23d and a fifth container 23e, and the second container group includes a sixth container 23f, a seventh container 23g and an eighth container 23g. Among them, the first container 23a, the second container 23b, the third container 23c, the fourth container 23d and the fifth container 23e are all detachably mounted on the first fixed seat 221, and the first container 23a, the second container 23b, the third container 23c, the fourth container 23d and the fifth container 23e are all provided with a first connecting channel for communicating with the first accommodating cavity, and each first connecting channel is provided with a first control valve for controlling the opening and closing of the corresponding first connecting channel. The sixth container 23f, the seventh container 23g and the eighth container 23g are all detachably mounted on the second fixed seat 22, and the sixth container 23f, the seventh container 23g and the eighth container 23g are all provided with a second connecting channel for communicating with the second accommodating cavity, and each second connecting channel is provided with a second control valve for controlling the opening and closing of the corresponding second connecting channel.

[0069] In this embodiment, the first container 23a is used for cell activation, the second container 23b is used for filtering the activated cells, the third container 23c is used for gene transduction of the cells after filtering through the second container 23b, the fourth container 23d is used for filtering the gene-transduced cells, the fifth container 23e is used for amplifying the gene-transduced cells, the sixth container 23f is used to store culture medium, the seventh container 23g is used to wash and filter the cells that fail the culture, and the eighth container 23g is used to store the washing solution.

[0070] In the present application, a magnetic attraction structure 16 is provided on the main body 10, and the magnetic attraction structure 16 is located above the first container 23a. In actual operation, the magnetic attraction structure 16 can adsorb magnetic beads such as CD3 / CD28 in the first container 23a. Optionally, the magnetic attraction structure 16 can be, for example, a magnet. Figure 1 , Figure 2 , Figure 3 , Figure 6 , Figure 7 as well as Fig.18 As shown, when actually preparing cells, firstly, the first container 23a, the second container 23b, the third container 23c, the fourth container 23d and the fifth container 23e are mounted on the first fixing seat 221, so that the connecting channels 233 on the first container 23a, the second container 23b, the third container 23c, the fourth container 23d and the fifth container 23e are all connected to the first accommodating cavity on the first fixing seat 221. The sixth container 23f, the seventh container 23g and the eighth container 23h are mounted on the second fixing seat 222, so that the connecting channels 233 on the sixth container 23f, the seventh container 23g and the eighth container 23h are connected to the second accommodating cavity on the second fixing seat 222. At this time, the separated T cells are injected into the first container 23a, and the driving member drives the rotating shaft 21 to slowly swing at 0° to 270° to drive each container 23 and the fixing seat 22 to slowly rotate along with the rotating shaft 21.

[0071] After injecting a cell solution containing a culture medium into the first container 23a and activating the cells in the first container 23a, the control valve of the first container 23a is controlled to open the connecting channel 233 of the first container 23a, so that the activated cells can flow into the first accommodating cavity through the first container 23a; then the control valve on the second container 23b is controlled to open the connecting channel 233 on the second container 23b, so that the cell solution in the first accommodating cavity flows into the second container 23b for filtration, and thereafter, the filtered cell solution is released again into the first accommodating cavity on the first fixing seat 221. Next, a control valve on the third container 23c is controlled by a controller or other structure to open the connecting channel 233 on the third container 23c, so that the virus solution in the third container 23c flows to the first containing cavity on the first fixed seat 221 and mixes with the filtered cell solution and culture medium for 24 hours for gene transduction. Thereafter, the fourth container 23d is controlled to open the connecting channel 233 thereon, and the solution after gene transduction is released into the fourth container 23d. After the culture medium is filtered out in the fourth container 23d, it is released into the first containing cavity on the first fixed seat 221, and then released from the first containing cavity into the fifth container 23e for cell amplification.

[0072] The cells need to be expanded in the fifth container 23e for 7-10 days. After a period of cell expansion, once it is detected that the culture medium in the fifth container 23e is insufficient, the control valve in the sixth container 23f is controlled to open the connecting channel 233 in the sixth container 23f. In this way, the culture medium in the sixth container 23f can flow into the first accommodating cavity through the second accommodating cavity and then into the fifth container 23e, ensuring the nutrients for cell culture and thus ensuring cell expansion.

[0073] After the cells have been expanded to a certain extent, the cells that meet the requirements can be collected according to actual needs. For cells that do not meet the requirements, by controlling the control valve of the seventh container 23g to open the connecting channel 233 of the seventh container 23g, the cells that do not meet the requirements in the fifth container 23e can be released into the seventh container 23g. At the same time, the cleaning liquid in the eighth container 23h, for example, sodium chloride solution, flows into the seventh container 23g through the second accommodating cavity. The sodium chloride solution in the eighth container 23h can be used to clean and filter the cells in the seventh container 23g, and then discharged. In the actual design process, the structures of the first container 23a, the second container 23b, the third container 23c, the fourth container 23d, the fifth container 23e, the sixth container 23f, the seventh container 23g and the eighth container 23g are basically the same. Specifically, the first container 23a, the second container 23b, the third container 23c, the fourth container 23d, the fifth container 23e, the sixth container 23f, the seventh container 23g and the eighth container 23g all include a semicircular body 230, a cover 234 and a sealing ring (not shown in the figure). The second container 23a, the fourth container 23a and the seventh container 23g are provided with a filter partition 236, and the filter partition 236 separates the accommodating cavity 231 of the corresponding container 23 into a first cavity 23b1 and a second cavity 23b2. That is to say, the first container 23a, the third container 23c, the fifth container 23e, the sixth container 26f and the eighth container 23h in this embodiment are not provided with a filter partition 236, while the second container 23a, the fourth container 23a and the seventh container 23g are provided with a filter partition 236. By providing the filter partition 236 (such as Figure 5 and Figure 6 As shown), part of the substances in the cells can be filtered to obtain a cell solution that meets the requirements.

[0074] The accommodating chamber 231 is divided into a first cavity 23b1 and a second cavity 23b2 by a filtering partition 236, so that the cells before and after filtration are respectively placed in the first cavity 23b1 and the second cavity 23b2. Among them, the first channel 2331 is connected to the first cavity 23b1, and the second channel 2332 is connected to the second cavity 23b2, and the above-mentioned control valves are arranged in the first channel 2331 and the second channel 2332. In this way, the opening and closing of the first channel 2331 and the second channel 2332 can be controlled respectively, so as to facilitate the release of the cell solution in a suitable actual situation.

[0075] In order to facilitate the transportation of substances, the cell therapy device in this embodiment also includes a connecting pipe (not shown in the figure), which is used to connect the first accommodating cavity and the second accommodating cavity, and the connecting pipe can be embedded in the rotating shaft 21, or can be set outside the rotating shaft 21. In this way, the culture medium in the sixth container 23f can flow from the second accommodating cavity to the first accommodating cavity through the connecting pipe, and then flow from the first accommodating cavity to the fifth container 23e, ensuring the nutrients for cell culture, thereby ensuring the expansion of cells. The connecting pipe can also transport the cells that do not meet the requirements in the sixth container 23f to the seventh container 23g, and use the sodium chloride solution in the eighth container 23h to wash and discharge the cells in the seventh container 23g.

[0076] It can be seen from the above working process that the first container 23a, the second container 23b, the third container 23c, the fourth container 23d, the fifth container 23e, the sixth container 23f, the seventh container 23g and the eighth container 23h are arranged in sequence along the length direction of the rotating shaft 21, and the processes of cell activation, gene transduction, cell amplification, cell cleaning, etc. can be completed in an orderly manner, thereby improving work efficiency.

[0077] Optionally, the driving member in this embodiment is a driving motor. When the driving motor drives the rotating shaft 21 to slowly swing at 0° to 270°, it can drive the solutions in each container to effectively mix and contact, thereby accelerating processes such as cell activation, gene transduction, and cell amplification.

[0078] Specifically, see Figure 4 As shown, each container 23 in this embodiment includes a semicircular body 230, a cover 234 and a sealing ring. The semicircular body 230 is surrounded to form a receiving cavity 231, and an opening 232 communicating with the receiving cavity 231 is provided on the semicircular body 230. The semicircular body 230 is detachably mounted on the fixing seat 22. The cover 234 is detachably covered on the semicircular body 230 to open or close the opening 232. The sealing ring is sleeved on the cover 234 to seal the opening 232.

[0079] When the container 23 is actually used to culture cells, the cover 234 is placed on the semicircular body 230 to close the opening 232 to prevent the cell solution from leaking out of the accommodating chamber 231 during the culture process. The semicircular body 230 is connected to the fixing seat 22. At this time, the cells to be cultured are added to the accommodating chamber 231, and the driving member drives the rotating shaft 21 to slowly swing in the working chamber 101, thereby driving the container 23 and the fixing seat 22 to slowly swing with the rotating shaft 21. In this way, the required cells can be cultured in the cell therapy device. After the culture is completed, the cell solution is collected in the cell therapy device, and then the semicircular body 230 can be removed from the fixing seat 22. In this way, the container 23 can be removed from the fixing seat 22. The removed container 23 does not need to be cleaned and can be directly thrown away, which can achieve the use of disposable consumables, reduce the cell infection rate during the culture process, and has a simple structure and is easy to use.

[0080] Furthermore, the fixing seat 22 in this embodiment is a semicircular columnar structure, and the semicircular columnar structure and the semicircular main body 230 are arranged to form a columnar structure and fixed to the outer periphery of the rotating shaft 21, which has a simple structure and is stable and reliable. In addition, by making the semicircular columnar structure and the semicircular main body 230 arranged to form a columnar structure, when the rotating shaft 21 swings, the cell solution can be more easily flowed from the container 23 into the accommodating cavity, and then enter the next container 23 from the accommodating cavity to complete the preparation of cells. Of course, in other embodiments of the present application, the container 23 can also be set as a square main body or other special-shaped main body, and the fixing seat 22 is set as a square columnar structure or other special-shaped columnar structure. As long as it is within the concept of the present application, it is within the protection scope of the present application.

[0081] In some embodiments, the fixing seat 22 and the container 23 are detachably connected via a snap assembly, which has a simple structure and is easy to assemble and disassemble.

[0082] Specifically, the buckle assembly in this embodiment includes a locking protrusion 31 and a locking groove (not shown in the figure) that cooperate with each other, and one of the locking groove and the locking protrusion 31 is set on the fixing seat 22, and the other is set on the container 23. In other words, when the locking groove is set on the fixing seat 22, the locking protrusion 31 is set on the container 23; when the locking groove is set on the container 23, the locking protrusion 31 is set on the fixing seat 22, and the structure is simple and easy to implement.

[0083] In a specific embodiment of the present application, the card slot is arranged on the fixed seat 22, and the card slot extends in a direction parallel to the length of the rotating shaft 21, and the card protrusion 31 is an elastic card protrusion, and the elastic card protrusion is detachably connected to the card slot. In this way, when it is necessary to culture the cell solution, the card protrusion 31 is connected to the card slot so that the container 23 is connected to the fixed seat 22; when the culture and collection are completed, the card protrusion 31 is removed from the card slot, and the used container 23 can be thrown away, so that the consumables can be used once, which reduces the cell infection rate during the culture process, and has a simple structure and is easy to use.

[0084] Of course, in other embodiments of the present application, the fixing seat 22 and the container 23 may be detachably connected via a magnetic attraction assembly. The magnetic attraction assembly includes two magnets, which are respectively arranged on the fixing seat 22 and the container 23. The container 23 may be simply and quickly fixed to the fixing seat 22 through the mutual attraction of the two magnets.

[0085] Specifically, the semicircular body 230 includes a first plane 2301, and a relief groove 235 is provided on the first plane 2301. The relief groove 235 extends along the midline of the first plane 2301. There are multiple clamping protrusions 31 and multiple clamping slots. The multiple clamping slots are connected to the multiple clamping protrusions 31 in a one-to-one correspondence, and the multiple clamping protrusions 31 are arranged on both sides of the relief groove 235. It can be understood that the midline of the first plane 2301 described in this embodiment refers to a line extending along the axis direction of the semicircular body 230 and dividing the first plane 2301 into two lines with the same area. By providing the relief groove 235, the rotating shaft 21 is partially embedded in the relief groove 235, so that the clamping protrusions 31 on both sides are connected to the fixing seat 22. In addition, by providing multiple clamping protrusions 31 and clamping slots, the connection stability between the cell container and the fixing seat 22 is greatly improved.

[0086] Furthermore, the protrusion 31 is a long strip protrusion, which extends along the length direction of the avoidance groove 235, and the protrusion 31 is an elastic protrusion. By setting the protrusion 31 as a long strip protrusion, the area of ​​connection between the container 23 and the fixing seat 22 is increased, and the connection stability between the semicircular body 230 and the fixing seat 22 is further improved.

[0087] Further, see Figure 4As shown, in a specific embodiment of the present application, there are two clamping protrusions 31, and the two clamping protrusions 31 are respectively arranged on both sides of the avoidance groove 235. In this way, when the clamping protrusion 31 is connected to the fixing seat 22, relative to the rotating shaft 21, the fixing seat 22 has clamping protrusions 31 connected on both the upper and lower sides, which further improves the connection stability between the cell container and the fixing seat 22. Of course, in other embodiments, the number of clamping protrusions 31 can also be set to three, four or five, or the clamping protrusions 31 can be set on the same side of the avoidance groove 235. The specific number of settings and design positions are determined according to actual usage conditions. As long as they are within the concept of the present application, they are within the protection scope of the present application.

[0088] Furthermore, an annular flange (not shown in the figure) is provided on one side of the cover 234 close to the semicircular body 230, and a sealing ring (not shown in the figure) is sleeved on the annular flange. In this way, the sealing ring can be sleeved on the semicircular body 230 to seal the gap between the semicircular body 230 and the opening 232, thereby strengthening the sealing between the cover 234 and the opening 232.

[0089] Specifically, the semicircular body 230 and the cover 234 are both transparent parts, which makes it easier for users to observe the changes of cells in the cell container.

[0090] Furthermore, at least one collecting channel 237 connected to the containing chamber 231 is provided on the semicircular body 230. That is, the collecting channel 237 may be one, two, or more than two. By providing the collecting channel 237, cells, culture medium, cleaning solution, etc. may be injected into the containing chamber 231 through the collecting channel 237 by a syringe or the like to perform the next operation and / or discharge the cell solution cultured in the containing chamber 231.

[0091] Furthermore, the multiple containers 23 in this embodiment are arranged in sequence along the length direction of the rotating shaft 21, and along the length direction of the rotating shaft 21, the cross-sectional area of ​​each container 23 gradually decreases, and the minimum cross-sectional area of ​​the previous container 23 is greater than the maximum cross-sectional area of ​​the next container 23. This arrangement avoids the situation where the container 23 is installed incorrectly or in the wrong position, and can play an anti-mistake function.

[0092] Combination Figure 8 , Fig. 9 as well as Fig.10 As shown, in order to facilitate the collection of cell solution, a cell outlet pipe 12 and a collection chamber 13 are provided on the main body 10 of the cell therapy device in this embodiment. One end of the cell outlet pipe 12 is connected to the fifth container 23e, and the other end of the cell outlet pipe 12 is connected to the collection chamber 13 and extends into the collection chamber 13. The cell outlet pipe 12 is provided with an outlet port 121, and an outlet switch 1211 is provided at the outlet port 121.

[0093] The cell therapy device also includes: a collection rack 40 and a collection bag 50, wherein the collection rack 40 can be slidably installed on the collection bin 13, and the collection rack 40 has a first position for sliding into the collection bin 13 and a second position for sliding outside the collection bin 13; the collection bag 50 can be detachably installed on the collection rack 40, and when the collection rack 40 is in the first position, the inlet of the collection bag 50 is aligned with the liquid outlet 121.

[0094] In this embodiment, the cell outlet pipe 12 is at least partially arranged in the collection chamber 13, so that the cells in the fifth container 23e can be released from the cell outlet pipe 12 into the collection chamber 13; a liquid outlet switch 1211 is arranged at the liquid outlet 121, and the opening and closing of the liquid outlet 121 can be controlled by the liquid outlet switch 1211. Secondly, the collection bag 50 is detachably mounted on the collection rack 40; the collection rack 40 is slidably mounted on the collection chamber 13, and the collection rack 40 has a first position for sliding into the collection chamber 13 and a second position for sliding out of the collection chamber 13; when the collection rack 40 is in the collection chamber 13, that is, in the first position, it is convenient to collect the cell solution; when the collection rack 40 is outside the collection chamber 13, that is, in the second position, it is convenient to take and put the collection bag 50.

[0095] When actually collecting the cells in the fifth container 23e, firstly, one end of the cell outlet pipe 12 is connected to the fifth container 23e, and the collection bag 50 is installed on the collection rack 40. Secondly, the collection rack 40 is slid so that the collection rack 40 is located in the collection bin 13 (i.e., the first position), and the entrance of the collection bag 50 is aligned with the liquid outlet 121. At this time, by controlling the liquid outlet switch 1211 to open the liquid outlet 121, the cells in the fifth container 23e can flow into the collection bag 50 through the cell outlet pipe 12 to realize the collection of the cell solution, etc. After the collection is completed, the collection rack 40 is slid again so that the collection rack 40 is located outside the collection bin 13 (i.e., the second position), and the collection bag 50 can be removed from the collection rack 40. The structure is simple, it is easier to realize automatic control, and the manual labor intensity is low.

[0096] See again Fig. 9As shown, the collection bag 50 includes a bag body 51 and a connecting tube 52 connected to the bag body 51. The collection bag 50 is detachably mounted on the collection rack 40. The connecting tube 52 protrudes from the collection rack 40. When the collection chamber 13 is in the first position, the connecting tube 52 opens the liquid outlet switch 1211 so that the liquid in the cell liquid outlet pipeline 12 flows into the bag body 51. In actual use, when the collection rack 40 is slid so that the collection rack 40 is located in the collection chamber 13 (i.e., the first position), the protruding portion of the connecting tube 52 can abut against the liquid outlet switch 1211, thereby opening the liquid outlet 121. At this time, the inlet of the collection bag 50 is aligned with the liquid outlet 121, and the cells in the fifth container 23e can flow from the cell liquid outlet pipeline 12 into the bag body 51. The structure is simple and the operation is convenient.

[0097] In this embodiment, since the collection rack 40 is slidably installed in the collection bin 13, when the collection rack 40 slides into the collection bin 13, the portion of the connecting tube 52 protruding from the collection rack 40 presses against the liquid outlet switch 1211, and the liquid outlet 121 can be opened. At this time, the tube mouth of the connecting tube 52 is located at the liquid outlet 121, and the cell outlet pipe 12 releases the cells to be collected in the container 23 into the collection bag 50. The operation is simple and easy to use.

[0098] That is, in this embodiment, the collecting bag 50 is detachably placed in the avoidance space 41, and the connecting tube 52 is passed through the positioning hole 42 from the avoidance space 41. In actual use, it is only necessary to push the collecting rack 40 so that the collecting rack 40 is installed in the collecting chamber 13, and then drive the connecting tube 52 to open the liquid outlet switch 1211, so that the cells can be collected, which is simple to operate and convenient to collect.

[0099] Furthermore, an avoidance space 41 and a positioning hole 42 are provided on the collection rack 40, and the positioning hole 42 is connected to the avoidance space 41, and the collection bag 50 is detachably placed in the avoidance space 41, and the connecting pipe 52 is detachably passed through the positioning hole 42 from the avoidance space 41 to protrude from the collection rack 40, wherein there are multiple positioning holes 42, and the multiple positioning holes 42 are arranged in sequence at intervals along the sliding direction of the collection rack 40.

[0100] Specifically, the collection rack 40 in this embodiment is a square rack, and there are a plurality of positioning holes 42, and the plurality of positioning holes 42 are sequentially spaced along the first direction of the collection rack 40 (i.e., the sliding direction of the collection rack 40). Specifically, the avoidance space 41 is a square avoidance space, and the plurality of positioning holes 42 are sequentially spaced along the sliding direction of the square avoidance space. Of course, in other embodiments of the present application, the collection rack 40 can also be set as a circular rack, an elliptical rack, or other special-shaped racks, as long as they are other deformation modes under the conception of the present application, they are all within the protection scope of the present application.

[0101] In this embodiment, by providing a plurality of positioning holes 42, the situation that the positioning holes 42 are damaged and cannot be used after long-term use of the collection rack 40 is avoided, and the service life of the collection rack 40 is greatly improved. The structure is simple and easy to implement. In addition, by providing a plurality of positioning holes 42, it is also possible to select according to actual use requirements, and then the connecting pipe 52 of the collection bag 50 can be connected to different positioning holes 42.

[0102] Optionally, in order to stably connect the collection bag 50 to the positioning hole 42, an external thread may be provided on the connecting tube 52, and an internal thread may be provided correspondingly at the positioning hole 42. When connected, the connecting tube 52 is connected to the positioning hole 42 through the matching internal and external threads, and is not easy to fall off. Of course, in other embodiments of the present application, a buckle assembly or other structures such as a limiting protrusion with a limiting function may be provided between the connecting tube 52 and the positioning hole 42. As long as it is other deformation methods under the conception of the present application, it is within the protection scope of the present application.

[0103] Specifically, the diameters of at least some of the plurality of positioning holes 42 are the same or different. It should be noted that the meaning of at least some of the plurality of positioning holes 42 having the same or different diameters is that, in actual design, the diameters of some of the plurality of positioning holes 42 may be different, the diameters of all the positioning holes 42 may be the same, or the diameters of all the positioning holes 42 may be different.

[0104] When the diameters of some of the multiple positioning holes 42 are set to be the same, while the diameters of the other parts are different, it is possible to accommodate collection bags 50 of various capacities; when the diameters of all the positioning holes 42 are set to be the same, it is possible to accommodate multiple collection bags 50 of the same capacity, thereby improving the collection efficiency of the cell collection mechanism; when the diameters of all the positioning holes 42 are set to be completely different, it is possible to accommodate collection bags 50 of various capacities.

[0105] This application is attached Fig. 9 In the embodiment, three positioning holes 42 are provided, and the diameters of the three positioning holes 42 are all different. Of course, four, five or six positioning holes 42 can also be provided, and the specific number and size of the positioning holes 42 are determined according to the actual use situation.

[0106] Specifically, the cell outlet pipe 12 is provided with a plurality of outlets 121 , and there are a plurality of collecting racks 40 , which are arranged side by side in the collecting chamber 13 , and the plurality of collecting racks 40 are arranged in one-to-one correspondence with the plurality of outlets 121 .

[0107] The liquid outlet 121 and the collecting rack 40 are arranged in plurality. When collecting cells, multiple collecting racks 40 can be used for collection at the same time, which greatly improves the collection efficiency. The structure is simple and easy to implement.

[0108] Optionally, the collection racks 40 in the present application can be set to two, three, four or five, etc., which are selected and designed according to actual usage requirements. Figure 8 FIG. 2 shows a case where three collecting racks 40 are provided.

[0109] like Fig.11 As shown, a slide groove 131 is provided on the side wall of the collection bin 13 in this embodiment. In actual use, the collection rack 40 can be slidably installed in the slide groove 131 to switch between the first position and the second position. The structure is simple and easy to use.

[0110] Specifically, both ends of the slide slot 131 are provided with stop blocks 132 for limiting the position of the collection rack 40. By providing the stop blocks 132, it is avoided that the collection rack 40 slides too fast and hits the collection bin 13, or the collection rack 40 falls when sliding out of the collection bin 13 and causes damage to the collection rack 40, thereby greatly improving the use stability of the collection rack 40.

[0111] For easy operation, the collection rack 40 in this embodiment is provided with a handle 43, which is pushed by an external force to slide the collection rack 40 along the collection bin 13. In actual use, by providing the handle 43, when cells need to be collected, it is only necessary to push the handle to slide the collection rack 40 into the collection bin 13. After the collection is completed, the collection rack 40 can be removed from the collection bin 13 by pulling the handle 43. The structure is simple and easy to use.

[0112] For example, the handle 43 in this embodiment is a protrusion or a buckle provided on the side of the collection rack 40. Of course, in other embodiments of the present application, the handle 43 can also be provided as other push-pull rods or other structures that facilitate pushing and pulling the collection rack 40. As long as it is other deformation methods under the conception of the present application, it is within the protection scope of the present application.

[0113] Furthermore, the bag body 51 in this embodiment is a transparent bag. By setting the bag body 51 as a transparent bag, it is convenient to observe the collection capacity of the cell solution. Optionally, the transparent bag is also provided with scale lines (not shown in the figure), so that accurate collection can be performed according to actual needs, and the structure is simple and easy to use.

[0114] Specifically, the bag body 51 in this embodiment is a flexible bag. Setting the bag body 51 as a flexible bag can avoid the bag body from being broken during use to a certain extent, greatly improving the service life of the collection bag 50.

[0115] Furthermore, the connecting tube 52 in this embodiment is a hard tube. In actual use, the connecting tube 52 passes through the positioning hole 42 from the avoidance space 41. When the collecting rack 40 is slid so that the collecting rack 40 is located in the collecting bin 13, the part of the connecting tube 52 that passes through the connecting tube 52 abuts against the liquid outlet switch 1211 to open the liquid outlet 121, so that the cell liquid in the cell liquid outlet pipe 12 can flow into the bag body 51. At this time, by setting the connecting tube 52 as a hard tube, it is convenient to abut against the liquid outlet switch 1211 to open the liquid outlet switch 1211.

[0116] In other embodiments of the present application, the liquid outlet switch 1211 can also be opened in other ways. Specifically, a detection element can be set in the collection chamber 13, and the cell therapy device includes a controller. The detection element is electrically connected to the controller. In actual use, the detection element is used to detect whether a collection bag 50 is placed on the collection rack when the collection rack is in the first position, and the controller controls the liquid outlet switch 1211 according to the signal transmitted by the detection element. Specifically, when the detection element detects that the collection rack 40 is in the first position and the collection bag 50 is placed on the collection rack 40, the controller receives the signal and controls the liquid outlet switch 1211 to open. In this way, the cell liquid in the cell outlet pipe 12 can flow from the liquid outlet 121 into the bag body 51. The structure is simple and the automatic collection of the collection bag 50 is realized. Correspondingly, when the detection element detects that there is no collection rack 40 in the collection chamber 13 or there is no collection bag 50 on the collection rack 40, the controller controls the liquid outlet switch 1211 to close to avoid cell leakage. Optionally, the detection element in this embodiment is a laser sensor or an infrared sensor or a detection camera and the controller is a control circuit board and other structures. As long as it is other deformation methods based on the concept of this application, they are within the protection scope of this application.

[0117] In other embodiments of the present application, the liquid outlet switch 1211 can also be opened by the user at an appropriate time according to his or her own usage needs, rather than being opened by pushing the collection rack 40 in. That is, when the collection rack 40 is slid so that the collection rack 40 is located in the collection bin 13 (i.e., the first position), the liquid outlet switch 1211 needs to be manually controlled to open the liquid outlet 121 on the cell liquid outlet pipe.

[0118] Furthermore, the liquid outlet switch 1211 in the present application can be set as an electric switch or a manual switch.

[0119] When the liquid outlet switch 1211 is set as an electric switch, it can be applied in the above three embodiments to open the liquid outlet 121, and is particularly suitable for the embodiment through the detection element. The controller controls the opening and closing of the electric switch so that the cells can flow from the liquid outlet 121 into the bag body 51. The structure is simple, easy to implement and has a high degree of automation. When the liquid outlet switch 1211 is set as a manual switch, it can be particularly applied in the case where the user chooses to open it at an appropriate time according to his or her own usage needs.

[0120] Combination Fig.12 and Fig.13 As shown, the cell therapy device in this embodiment also includes a status detection mechanism and a display screen (not shown in the figure). The status detection mechanism is arranged in the working chamber 101, and the status detection mechanism includes a shooting element 141; the display screen is installed on the main body 10, and the display screen is electrically connected to the shooting element 141 to display and / or store the picture taken by the shooting element 141.

[0121] When the cell therapy device is working, the shooting element 141 of the state detection mechanism can take real-time pictures of the working state of the cell therapy mechanism 20, and can transmit the pictures taken by the shooting element 141 to the display screen on the main body 10 for display and / or storage, so that the user can observe or retrieve them as needed. Compared with the manual observation method in the prior art, the shooting element 141 is used in this embodiment to take pictures of the state of the cell therapy mechanism 20, so that the user can observe and retrieve them at the appropriate time, with a higher degree of automation and low labor intensity, which can improve the efficiency of cell preparation.

[0122] In order to facilitate observation of the cell preparation process, the state detection mechanism bracket can be installed on the side wall of the working chamber 101 (such as Fig.13 A separate mounting bracket 142 may also be provided to mount the state detection mechanism (as shown in FIG. Fig.14 shown).

[0123] See also Fig.14 As shown, the mounting bracket 142 is fixedly connected to the main body 10 , and the photographing element 141 is disposed on the mounting bracket 142 , so as to facilitate supporting and mounting the photographing element 141 .

[0124] Furthermore, the mounting bracket 142 can be fixed on the main body 10, or can be movably mounted on the main body 10. When the mounting bracket 142 is fixed on the main body 10, the photographing element 141 is fixed on the mounting bracket 142 for photographing; when the mounting bracket 142 is movably mounted on the main body 10, the photographing element 141 can be moved and photographed according to the movement of the mounting bracket 142. As long as it is within the concept of this application, it is within the protection scope of this application.

[0125] Furthermore, in this embodiment, a slide rail 15 is provided on the main body 10, and the slide rail 15 extends along the length direction of the working chamber 101, and the mounting bracket 142 is slidably mounted on the slide rail 15. By providing the slide rail 15, the mounting bracket 142 can be moved on the main body 10, so as to facilitate the movement of the shooting element 141 for all-round shooting.

[0126] Furthermore, the state detection mechanism also includes a driving member (not shown in the figure), which is drivingly connected to the mounting bracket 142 to drive the mounting bracket 142 to slide along the slide rail 15. The structure is simple and automatic control is easy to achieve.

[0127] Furthermore, the photographing element 141 includes a camera. A camera generally has basic functions such as video recording / transmission and static image capture. The present application utilizes the camera's function of taking both pictures and videos for photographing.

[0128] See also Fig.15 and Fig.16 As shown, the main body 10 in this embodiment is further provided with a cover plate 11 and a mounting port 102, the cover plate 11 includes a first cover plate 11A and a second cover plate 11B, the mounting port 102 includes a first mounting port 1021 and a second mounting port 1022, the first mounting port 1021 is connected to the first working chamber 101a, and the second mounting port 1022 is connected to the second working chamber 101b. The first cover plate 11A is movably mounted on the main body 10 to open or close the first mounting port 1021, and the second cover plate 11B is movably mounted on the main body 10 to open or close the second mounting port 1022.

[0129] In this way, during the cell culture process, the first cover plate 11A is moved to close the first mounting port 1021, thereby sealing the first working chamber 101a; the second cover plate 11B is moved to close the second mounting port 1022, thereby sealing the second working chamber 101b, so as to better culture cells and preserve cleaning fluid and culture medium, while preventing external dust from entering the first working chamber 101a or the second working chamber 101b, so that the cell therapy device can stably perform its cell preparation actions.

[0130] Specifically, a bracket 113 is provided on one side of the first cover plate 11A close to the first working chamber 101 a and on one side of the second cover plate 11B close to the second working chamber 101 b , and the first cover plate 11A and the second cover plate 11B are both mounted on the main body 10 through the bracket 113 .

[0131] See also Fig.16As shown, an avoidance groove 103 is provided on the main body 10, and the avoidance groove 103 extends along the height direction of the main body 10; a slide rail assembly is provided between the avoidance groove 103 and the bracket 113, and a rotating part 1031 is provided at the top of the avoidance groove 103. After the cover plate is driven by an external force to move along the slide rail assembly to the top of the avoidance groove 103, it can rotate along the rotating part 1031 so that the cover plate rests on the top of the main body 10.

[0132] When the first mounting port 1021 or the second mounting port 1022 needs to be opened, the user applies an upward force to the first cover plate 11A or the second cover plate 11B. At this time, the bracket 113 can move upward along the slide rail assembly. When the bracket 113 moves to the top of the main body 10 along the slide rail assembly, the first cover plate 11A or the second cover plate 11B is pushed toward the back side of the main body 10 (away from the side of the first mounting port 1021 or the second mounting port 1022). At this time, the bracket 113 can rotate along the rotating portion 1031. When the bracket 113 rotates along the rotating portion 1031 toward the back side of the main body 10 and rests on the top of the main body 10, the first mounting port 1021 or the second mounting port 1022 can be fully opened. When the first installation opening 1021 or the second installation opening 1022 needs to be closed, it is only necessary to apply force to the first cover plate 11A or the second cover plate 11B to make the cover plate 11 rotate forward along the rotating portion 1031. When the bracket 113 on the first cover plate 11A or the second cover plate 11B can move downward along the slide rail assembly, the external force applied to the first cover plate 11A or the second cover plate 11B is released, and the first cover plate 11A or the second cover plate 11B can drive the bracket 113 to move downward along the slide rail assembly under its own gravity to close the first installation opening 1021 or the second installation opening 1022. The structure is simple and easy to operate. Optionally, the rotating portion 1031 in this embodiment is a rotating shaft.

[0133] Optionally, the slide rail assembly in this embodiment includes a track 11310 and a slider (not shown in the figure) that cooperate with each other, one of the track 11310 and the slider is set on the bracket 113, and the other is set in the avoidance groove 103. That is to say, when the track 11310 is set on the bracket 113, the slider is set in the avoidance groove 103; when the track 11310 is set in the avoidance groove 103, the slider is set on the bracket 113. By setting the slide rail assembly as a track 11310 and a slider that cooperate with each other, when the bracket 113 moves in the avoidance groove 103, the slider can also limit the bracket 113 to prevent the bracket 113 from deflecting, and the bracket 113 can move smoothly, with a simple structure and convenient operation.

[0134] Further, the bracket 113 in this embodiment includes a first support beam 1131, which extends in the height direction of the cover plate 11, and a track 11310 is disposed on the first support beam 1131 and extends in the length direction of the first support beam 1131. In a specific embodiment of the present application, the track 11310 is disposed on the first support beam 1131, and the slider is disposed in the avoidance groove 103. When the first support beam 1131 is extended in the height direction of the cover plate 11, it is more convenient for the user to push or pull the bracket 113 to move.

[0135] The first cover plate 11A and the second cover plate 11B both include a first plate segment 111 and a second plate segment 112, and the first plate segment 111 and the second plate segment 112 are arranged to form an L-shaped structure, and the first support beam 1131 is arranged perpendicular to the first plate segment 111. By arranging the first plate segment 111 and the second plate segment 112 to form an L-shaped structure, when the cover plate 11 is in a closed state, the cover plates 11 on both sides enclose the main body 10 to form a rectangular parallelepiped structure, so that the working chamber 101 can be covered. Of course, in other embodiments of the present application, the cover plate 11 can also be set as a flat plate structure that can close the installation port 102. As long as it is other deformation methods based on the concept of the present application, it is within the protection scope of the present application.

[0136] Furthermore, the bracket 113 also includes a second support beam 1132, which is connected between the second plate segment 112 and the first support beam 1131. By setting the second support beam 1132, the connection stability between the main body 10 and the cover plate 11 is further strengthened; at the same time, it can also withstand the gravity of the cover plate 11, avoiding the situation of unstable installation due to only the first support beam 1131 supporting the cover plate 11, greatly improving the service life of the entire cell therapy device, with a simple structure and easy implementation. Optionally, the second support beam 1132 can be set perpendicular to the first support beam 1131 and the second plate segment 112, or can be set obliquely to the first support beam 1131 and the second plate segment 112.

[0137] See also Fig.16As shown, the track 11310 in this embodiment is a waist-shaped groove, that is, a long groove, which is arranged on the first support beam 1131, and the rotating part 1031 includes a rotating shaft, which is arranged at the top of the avoidance groove 103 and penetrates the waist-shaped groove. When the installation port 102 is opened, the cover plate 11 and the bracket 113 are first pulled up. When the bottom end of the long groove moves to the rotating shaft, the cover plate 11 is pushed to the back of the main body 10. At this time, the bracket 113 can be rotated along the rotating shaft for a certain angle and then rest on the main body 10. The structure is simple and easy to use and operate. Optionally, there are multiple brackets 113 in the present application, and the multiple brackets 113 are arranged at intervals along the length direction of the main body 10 to facilitate stable support of the cover plate 11. In a specific embodiment of the present application, there are two brackets 113, wherein the two brackets 113 are respectively installed at the two ends of the cover plate 11, so that the service life of the entire cell therapy device can be greatly improved while strengthening the installation stability between the cover plate 11 and the main body 10.

[0138] Of course, in other embodiments of the present application, the brackets 113 can also be set to three or more. As long as they are other deformation methods based on the concept of the present application, they are within the protection scope of the present application.

[0139] Furthermore, the cover plate 11 in this embodiment is a transparent cover plate. By setting the cover plate 11 as a transparent cover plate, when the cells in the container 23 are cultured or processed, it is more convenient for the user or patient to observe the changes of the cells in the cell therapy device.

[0140] Furthermore, the cell therapy device further includes a support assembly, which is used to support the support 113 when the support 113 moves to the top of the main body 10. After the culture is completed, when the container needs to be removed from the cell therapy device and thrown away, the support assembly is provided to effectively prevent the support 113 from sliding down when it moves to the top of the main body 10 and closing the first installation port 1021 or the second installation port 1022.

[0141] Specifically, the support assembly in this embodiment includes a support block 1032 and an elastic element 1033 . The support block 1032 is disposed in the side wall of the avoidance groove 103 , and the elastic element 1033 abuts between the support block 1032 and the side wall of the avoidance groove 103 .

[0142] In actual use, when the first cover plate 11A or the second cover plate 11B drives the bracket 113 to move along the slide rail assembly to the top of the avoidance groove 103 under the action of external force, the bracket 113 stops at the outside of the support block 1032, and the support block 1032 compresses the elastic element 1033; when the first cover plate 11A or the second cover plate 11B moves to the top of the main body 10, the bracket 113 avoids the support block 1032, and the support block 1032 can extend into the avoidance groove 103 under the support of the elastic element 1033, and then support at the bottom of the bracket 113, preventing the cover plate 11 from sliding downward along the slide rail assembly, with a simple structure and easy to implement. Optionally, the support block 1032 in this embodiment is a wedge block, and the elastic element 1033 can be provided with a spring or a spring sheet or other structures.

[0143] See also Fig.17 and Fig.18 As shown, the control method of the cell therapy device includes the following steps:

[0144] Step S1: injecting a cell solution containing a culture medium into the first container 23a, and performing cell activation in the first container 23a.

[0145] Before performing step S1, first install the first container 23a, the second container 23b, the third container 23c, the fourth container 23d, the fifth container 23e, the sixth container 23f, the seventh container 23g and the eighth container 23g mentioned above on the first fixing seat 221 and the second fixing seat 222 respectively.

[0146] After completing the assembly of the first container 23a, the second container 23b, the third container 23c, the fourth container 23d, the fifth container 23e, the sixth container 23f, the seventh container 23g and the eighth container 23g, the preparation process of the CAR T cells can be performed.

[0147] Specifically, when preparing CAR T cells, T cells separated from human blood and culture medium for culturing T cells are first injected into the first container 23a, and at the same time, activation substances such as immunomagnetic beads are added to the first container. Specifically, they can be injected into the first container 23a through an injection port reserved on the first container 23a using a syringe or the like.

[0148] After the injection is completed, the controller is used to control the driving motor provided on the main body 10 to work, so as to drive the rotating shaft 21 to rotate, and then drive the first container 23a to swing back and forth at a first predetermined speed for a first predetermined time, so as to activate the cells uniformly. In this step, the rotation angle of the rotating shaft 21 is 0° to 270°, and the specific swing angle can be adjusted according to the mixing and activation of the cell solution.

[0149] The first predetermined speed in this embodiment can also be adjusted according to the activation of the cells and the mixing of the cells with the culture medium and other substances. Optionally, the first container 23a or the first fixed seat 221 in this embodiment is provided with a sensor for detecting the temperature, cell activation status, etc. in the first container 23a, and the detected signal is transmitted to the controller by the sensor, so that the controller can control the driving motor to adjust the rotation angle and rotation speed of the rotating shaft 21. In view of the different activation conditions of the cells, the first predetermined speed and the rotation angle of the rotating shaft 21 are not limited in this application. The specific values ​​can be set on the controller by using the control program after experimental verification, and will not be specifically introduced in this application.

[0150] Furthermore, in this embodiment, the first predetermined time is 44h to 54h, such as 44h, 46h, 48h, 50h or 54h. It can be selected and set according to the properties of the cells to be activated. Taking the preparation of CAR T cells as an example, the first predetermined time is usually 48h.

[0151] Step S2: Release the cell solution in the first container 23a into the second container 23b for filtration, release the cell solution after filtering out the culture medium into the third container 23c, and inject the virus solution and new culture medium into the third container 23c for cell gene transduction.

[0152] Since the culture medium will change or deteriorate during the cell activation process, after completing the cell activation, it is necessary to filter out the culture medium in the first container 23a. The specific operation is that when the driving motor drives the rotating shaft 21 to work for a first predetermined time, the controller controls the first control valve in the first connecting channel on the first container 23a to open, and releases the cell solution in the first container 23a into the first accommodating cavity of the first fixing seat 221, and at the same time controls the first control valve in the first connecting channel on the second container 23b to open, so that the cell solution in the first accommodating cavity is released into the second container 23b. After the cell solution enters the second container 23b, the culture medium in the cell solution can be filtered out through the filtering effect of the filtering partition 236. After completing the filtration of the culture medium, the controller controls the first control valve in the first connecting channel on the second container 23b to open, and releases the filtered cell container into the first accommodating cavity, and at the same time controls the first control valve in the first connecting channel on the third container 23c to open, and releases the filtered cell solution into the third container 23c.

[0153] At this time, the third container 23c is provided with an injection port, through which the virus solution used for gene transduction of cells and new culture medium can be injected into the third container 23c, so that the culture medium, virus solution and activated cell solution can coexist in the third container 23c for gene transduction.

[0154] It is understandable that, in this step, the virus solution and the culture medium may be pre-loaded into the third container 23c, or may be injected after the cell solution enters the third container 23c.

[0155] After the virus solution, cell solution and new culture medium are mixed, the controller is used to control the driving motor to drive the rotating shaft 21 to rotate, thereby driving the third container 23c to swing at a second predetermined speed for a second predetermined time, so that the culture medium, virus solution and cell solution can be effectively contacted, the speed of gene transduction can be accelerated, and the success rate of gene transduction can be improved.

[0156] In this step, the second predetermined speed can also be adjusted according to the cell gene transduction condition and the mixing condition with the culture medium and other substances. Optionally, the third container 23c or the first fixing seat 221 in this embodiment is provided with a sensor for detecting the temperature in the first container 23c, the cell gene transduction condition, etc., and the sensor transmits the detected signal to the controller, so that the controller can control the driving motor to adjust the rotation speed of the rotating shaft 21. In view of the different gene transduction conditions of different cells, the second predetermined speed is not limited in this application.

[0157] Furthermore, in this embodiment, the second predetermined time is 20 hours to 28 hours, for example, 20 hours, 24 hours, and 28 hours. It can be selected and set according to the properties of the cells to be gene-transduced. For example, for the preparation of CAR T cells, the second predetermined time is usually 24 hours.

[0158] Step S3: releasing the cell solution that has completed gene transduction into the fourth container 23d for filtration, and releasing the cell solution after filtering out the culture medium into the fifth container 23e for cell amplification, and regularly checking whether the amplified cells in the fifth container 23e are qualified.

[0159] In this step, after the controller controls the driving motor to work for the second predetermined time, the controller controls the first control valve in the first connecting channel of the third container 23c to open, and releases the solution in the third container 23c into the first accommodating cavity of the first fixing seat 221, and at the same time controls the first control valve in the first connecting channel of the fourth container 23d to open, so that the solution in the first accommodating cavity enters the fourth container 23d to filter out the changed or deteriorated culture medium. After the filtration is completed, the controller controls the first control valve in the first connecting channel on the fourth container 23d to open, and releases the filtered cell solution into the first accommodating cavity, and at the same time controls the first control valve in the first connecting channel on the fifth container 23e to open, so that the cell solution in the first accommodating cavity enters the fifth container 23e for amplification. At this time, a certain culture medium is contained in the fifth container 23e, which is convenient for culturing the cells after gene transduction so that the cells can be amplified.

[0160] In this step, the controller is used to control the rotation of the rotating shaft 21, thereby driving the fifth container 23e to swing at a third predetermined speed for a third predetermined time. By controlling the swing of the rotating shaft 21, the fifth container 23e can be driven to swing, and the culture medium and cell solution in the fifth container 23e can be effectively mixed, thereby improving the cell proliferation rate and the success rate of cell culture.

[0161] Similarly, the third predetermined speed can also be adjusted according to the cell proliferation conditions and the mixing conditions of the cells with the culture medium and other substances. Optionally, the fifth container 23e or the first fixing seat 221 in this embodiment is provided with a sensor for detecting the temperature, cell proliferation conditions, etc. in the fifth container 23e, and the detected signal is transmitted to the controller by the sensor, so that the controller can control the driving motor to adjust the rotation speed of the shaft 21. In view of the different gene growth conditions of different cells, the third predetermined speed is not limited in this application.

[0162] Optionally, the third predetermined time is 7d to 10d, such as 7d, 8d, 9d or 10d. It can be selected and set according to the properties of the cells to be amplified as needed. Taking the preparation of CAR T cells as an example, the third predetermined time is usually 7d.

[0163] In step S3, after a period of cell expansion, the culture medium in the fifth container 23e is insufficient to support cell expansion. When it is detected that the culture medium in the fifth container 23e is lower than a predetermined amount, the controller can be used to release the culture medium in the sixth container 23f into the fifth container 23e.

[0164] Specifically, when the sensor and other structures detect that the culture medium in the fifth container 23e is lower than a predetermined amount (the predetermined amount is related to the volume of the fifth container and the initial amount of the cell solution, and is not specifically limited in the present application), the controller is used to control the second control valve in the second connecting channel on the sixth container 23f to open, and at the same time, the first control valve in the first connecting channel on the fifth container 23e is controlled to open. Since the first accommodating cavity and the second accommodating cavity are connected, at this time, the culture medium in the sixth container 23f can pass through the second accommodating cavity on the second fixed seat 222 and the first accommodating cavity on the first fixed seat 221 in sequence and enter the fifth container 23e. The structure is simple and easy to implement.

[0165] In this embodiment, the fifth container 23e is provided with a sampling port, through which the cell solution in the fifth container 23e can be sampled and tested regularly. When the cells in the fifth container 23e are found to be qualified, they can be collected. When the cells in the fifth container 23e are always unqualified, they can be processed and discharged. The regular testing here can be, for example, sampling once a day for testing.

[0166] Step S4: using a collection bag to collect the qualified cell solution in the fifth container.

[0167] This step is used to collect the cell solution after the test is qualified. When it is necessary to collect cells, the collection bag 50 is placed on the collection rack 40, and the collection rack 40 is pushed into the collection bin 13. At this time, the hard pipe joint at the top of the collection bag 50 can open the switch at the end of the cell outlet pipe 12, and the cell solution in the fifth container 23e can flow from the cell outlet pipe into the collection bag 50 to achieve collection.

[0168] Step S5: releasing the unqualified cell solution in the fifth container 23e into the seventh container 23g, and releasing the cleaning solution in the eighth container 23h into the seventh container 23g to clean and filter the cell solution in the seventh container 23g.

[0169] This step is used to clean and filter the cell solution that fails the test. That is to say, when the cell solution is amplified in the fifth container 23e for a period of time, multiple sampling tests are unqualified (i.e., the sampling is unqualified after the third predetermined time of cultivation), the controller is used to control the first control valve in the first connecting channel on the fifth container 23e to open, and the second control valve in the second connecting channel on the seventh container 23g is controlled to open. Since the first accommodating cavity and the second accommodating cavity are connected, at this time, the cell solution in the fifth container 23e can pass through the first accommodating cavity and the second accommodating cavity in sequence and enter the seventh container 23g. After that, the controller is used to control the second control valve in the second connecting channel on the eighth container 23h and the seventh container 23g to open at the same time, so that the cleaning solution (sodium chloride solution) in the eighth container 23h enters the seventh container 23g to clean and filter the cell solution, and the cell solution after cleaning and filtration can be discharged from the discharge port reserved on the seventh container 23g.

[0170] According to the above description, the technical solution of the present application has at least the following technical effects:

[0171] The control method of the cell therapy device of the present application can perform highly automated and highly integrated control of processes such as cell activation, cell gene transduction, cell expansion, cell collection, cell cleaning and discharge, greatly reducing the intensity of manual labor, shortening the cell preparation process, and improving the efficiency and yield of cell preparation.

[0172] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figure. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figure. For example, if the device in the accompanying drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below other devices or structures". Thus, the exemplary term "above" can include both "above" and "below". The device can also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.

[0173] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of this application.

[0174] The above are only preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A cell therapy device, characterized in that: include: A main body (10), wherein the main body (10) is provided with a working chamber (101), a cell outlet pipe (12) and a collection chamber (13); A cell therapy mechanism (20), the cell therapy mechanism (20) comprising a driving member, a rotating shaft (21) and a container assembly, the rotating shaft (21) being rotatably arranged in the working chamber (101), the driving member being arranged on the main body (10) and drivingly connected to the rotating shaft (21) so as to drive the rotating shaft (21) to swing in the working chamber (101), and the container assembly being detachably fixed on the rotating shaft (21) so as to at least perform cell activation, gene transduction, cell amplification and cell washing; A cell collection mechanism is arranged on the main body (10), one end of the cell outlet pipe (12) is connected to the container assembly, and the other end of the cell outlet pipe (12) is connected to the collection chamber (13) and extends into the collection chamber (13) to be connected to the cell collection mechanism.

2. The cell therapy device according to claim 1, characterized in that: A fixing seat (22) is fixedly mounted on the rotating shaft (21), and a receiving cavity is provided in the fixing seat (22); The container assembly comprises a plurality of containers (23), wherein the plurality of containers (23) are arranged in sequence along the length direction of the rotating shaft (21), and each of the containers (23) is provided with a connecting channel (233) for communicating with the accommodating cavity, and the connecting channel (233) is provided with a control valve for controlling the opening and closing of the connecting channel (233).

3. The cell therapy device according to claim 2, characterized in that: The fixing seat (22) comprises a first fixing seat (221), a first accommodating cavity is arranged on the first fixing seat (221), the container assembly comprises a first container (23a), a second container (23b), a third container (23c), a fourth container (23d) and a fifth container (23e) arranged in sequence along the length direction of the rotating shaft (21), the first container (23a), the second container (23b), the third container (23c), the fourth container (23d) and the fifth container (23e) are all provided with the connecting channel (233) communicating with the first accommodating cavity, wherein: The first container (23a) is used for cell activation, and a magnetic attraction structure (16) is provided on the main body (10), and the magnetic attraction structure (16) is located above the first container (23a); The second container (23b) is used to filter the activated cells; The third container (23c) is used to store the virus solution; The fourth container (23d) is used to filter the cell solution after gene transduction; The fifth container (23e) is used to amplify the cells after filtration.

4. The cell therapy device according to claim 3, characterized in that: The fixing seat (22) comprises a second fixing seat (222), and a second accommodating cavity is arranged on the second fixing seat (222). The container assembly also comprises a sixth container (23f), a seventh container (23g) and an eighth container (23h) which are arranged in sequence along the length direction of the rotating shaft (21). The sixth container (23f), the seventh container (23g) and the eighth container (23h) are all located on a side of the fifth container (23e) away from the fourth container (23d). The sixth container (23f), the seventh container (23g) and the eighth container (23h) are all provided with the connecting channel (233) which is communicated with the second accommodating cavity, wherein: The sixth container (23f) is used to store culture medium; The seventh container (23 g) is used to wash the cell solution; The eighth container (23h) is used to store cleaning liquid.

5. The cell therapy device according to claim 4, characterized in that: The cell therapy device further comprises a connecting pipe, the connecting pipe being used to connect the first accommodating cavity and the second accommodating cavity, and the connecting pipe being embedded in the rotating shaft (21) or arranged outside the rotating shaft (21).

6. The cell therapy device according to claim 3, characterized in that: One end of the cell liquid outlet pipe (12) is connected to the fifth container (23e), and the other end of the cell liquid outlet pipe (12) is connected to the collection chamber (13) and extends into the collection chamber (13). The cell liquid outlet pipe (12) is provided with a liquid outlet (121), and a liquid outlet switch (1211) is provided at the liquid outlet (121); The cell collection mechanism comprises: A collecting rack (40), the collecting rack (40) being slidably mounted on the collecting bin (13), and the collecting rack (40) having a first position for sliding into the collecting bin (13) and a second position for sliding out of the collecting bin (13); A collecting bag (50), wherein the collecting bag (50) is detachably mounted on the collecting rack (40); when the collecting rack (40) is in the first position, the inlet of the collecting bag (50) is aligned with the liquid outlet (121).

7. The cell therapy device according to claim 6, characterized in that: The collecting bag (50) comprises a bag body (51) and a connecting tube (52) connected to the bag body (51); the collecting bag (50) is detachably mounted on the collecting rack (40); the connecting tube (52) protrudes from the collecting rack (40); and when the collecting bin (13) is in the first position, the connecting tube (52) opens the liquid outlet switch (1211) to allow the liquid in the cell liquid outlet pipe (12) to flow into the bag body (51).

8. The cell therapy device according to claim 7, characterized in that: The collecting bag (50) is detachably mounted on the collecting rack (40), the connecting tube (52) protrudes from the collecting rack (40), and the connecting tube (52) opens the liquid outlet switch (1211) when the collecting bin (13) is in the first position so that the liquid in the cell liquid outlet pipe (12) flows into the bag body (51).

9. The cell therapy device according to claim 6, characterized in that: A detection element is arranged in the collection bin (13), and the cell therapy device comprises a controller. The detection element is electrically connected to the controller. The detection element is used to detect whether the collection bag (50) is placed on the collection rack when the collection rack is in the first position. The controller controls the liquid outlet switch (1211) according to the signal transmitted by the detection element.

10. The cell therapy device according to claim 6, characterized in that: The cell outlet pipe (12) is provided with a plurality of the liquid outlets (121), and the collecting racks (40) are multiple, and the multiple collecting racks (40) are arranged side by side in the collecting bin (13), and the multiple collecting racks (40) are arranged in a one-to-one correspondence with the multiple liquid outlets (121).

11. The cell therapy device according to any one of claims 1 to 10, characterized in that: The driving member is a driving motor, and the driving motor is drivingly connected to the rotating shaft (21) to drive the rotating shaft (21) to rotate within a range of 0° to 270°.

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