Microbial fermentation combined culture device

Abstract

The invention discloses a microbial fermentation combined culture device, and relates to the technical field of microbial culture, the microbial fermentation combined culture device comprises a chassis and also comprises an annular culture box, the annular culture box is fixedly mounted on the top surface of the chassis, a plurality of culture cavities are formed in the annular culture box, culture tanks and storage boxes are placed in the culture cavities, and the annular culture box is rotatably mounted on the top surface of the chassis; the storage box is arranged in the annular culture box and surrounded by the annular culture box, a plurality of independent liquid storage cavities communicated with the culture tanks are arranged on the periphery of the storage box, a mixed liquid storage cavity and a pushing and transferring mechanism are arranged in the middle of the storage box, and the pushing and transferring mechanism is installed in the storage box and used for conveying liquid in the independent liquid storage cavities into the culture tanks and the mixed liquid storage cavity. The mixed liquid storage cavity and the multiple independent liquid storage cavities are formed in the storage box capable of rotating, the pushing and transferring mechanism can feed liquid in the independent liquid storage cavities into the mixed liquid storage cavity and different culture tanks, and rapid supply and mixing of different culture solutions are achieved.

Description

Technical Field

[0001] The invention relates to the technical field of microbial cultivation, in particular to a microbial fermentation combined cultivation device. Background Art

[0002] Microbial fermentation refers to the process of using microorganisms to carry out metabolic reactions under suitable conditions to convert raw materials into products needed by humans. This process usually occurs in a specific fermentation culture device, which provides a controlled growth environment for microorganisms, including suitable temperature, pH value, nutrients, gas composition and other conditions. In microbial fermentation, microorganisms absorb nutrients and synthesize various useful compounds such as organic acids, alcohols, antibiotics, enzymes, etc. through specific metabolic pathways. These products have wide application value in the fields of medicine, food, chemical industry, environmental protection, etc.

[0003] The patent publication number of the existing patent application is: CN211645187U, and the publication date is October 9, 2020. The name of the patent is "A multi-species microbial cultivation device". The patent includes a cover bracket, a sealing rubber cover, an incubator group, an incubator, a temperature control bottom layer, a base, a heating side box and a press buckle plate. The base supports and installs the incubator group, and one side of the incubator group is connected to the heating side box. The interior of the incubator group is vertically installed with an insulation layer to divide the incubator group into several incubators. The bottom of the incubator is horizontally separated by a culture bottom, and the bottom of the culture bottom is the temperature control bottom layer. The cover bracket is fixedly installed on both sides of the base. The beneficial effect is that the multi-species microbial cultivation device described in the utility model can realize multi-species cultivation of microorganisms in each culture medium by grouping the incubators and adjusting the environmental differences in each group box, thereby reducing resource occupation and improving cultivation efficiency.

[0004] The above application has shortcomings. The current microbial culture device used for medical testing is mainly carried out by applying culture fluid to a single culture dish or culture tank placed in an incubator. This method is direct and fast, but can only meet the culture needs of a single culture dish for microorganisms, or place multiple culture dishes in the incubator and culture the microorganisms in multiple culture dishes as a whole. Although this method meets the requirements of batch culture, multiple culture dishes are in the same unsealed incubator, which is prone to contamination and affects the culture results. It is usually necessary to manually add liquid to the culture tank. When multiple liquids need to be mixed, the operation will be more cumbersome and easy to introduce contamination. Summary of the invention

[0005] The purpose of the present invention is to provide a microbial fermentation combined culture device to solve the deficiencies in the above-mentioned prior art.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A microbial fermentation combined culture device comprises a chassis and an annular culture box, which is fixedly mounted on the top surface of the chassis and is provided with a plurality of culture chambers in which culture tanks are placed; a storage box, which is rotatably mounted on the top surface of the chassis and is surrounded by the annular culture box; a plurality of independent liquid storage chambers connected to the culture tanks are arranged on the periphery of the storage box; a mixing liquid storage chamber is arranged in the middle of the storage box; a push-transport mechanism is installed in the storage box and is used to deliver liquid in the independent liquid storage chambers into the culture tanks and the mixing liquid storage chamber; a connecting port is arranged between the mixing liquid storage chamber and each independent liquid storage chamber; a plugging adjustment member matched with the connecting port is installed in the pushing-transport mechanism, and a one-way discharge pipe is installed between one of the connecting ports and the pushing-transport mechanism.

[0008] Preferably, an observation window corresponding to each culture tank is provided on the outer peripheral surface of the annular culture box, and a transparent panel is installed in the observation window.

[0009] Preferably, a telescopic connecting pipe is installed on the top of the independent liquid storage cavity, and a liquid injection nozzle for inserting the telescopic connecting pipe is installed on the top of the culture tank.

[0010] Preferably, a plurality of inner air chambers and a plurality of outer observation chambers connected thereto are distributed in a ring shape above the interior of the storage box, the pushing and transferring mechanism comprises a piston pressure plate movably installed in the inner air chamber and a piston top plate movably installed in the outer observation chamber, a piston support plate located in an independent liquid storage cavity is installed at the bottom of the piston top plate, and an adjusting pressure rod penetrating the top of the storage box is fixedly connected to the top of the piston top plate.

[0011] Preferably, the pushing and transferring mechanism also includes a hollow shaft fixedly inserted in the storage box, a twist cover is fixedly connected to the top of the hollow shaft, the blocking adjustment part includes a plurality of toggle frames vertically slidably connected to the inner side wall of the hollow shaft, a sealing plate is installed at the connecting port at the bottom end of the toggle frame, and a toggle rod is installed on the top surface of the twist cover at the top end of the toggle frame.

[0012] Preferably, an annular mounting seat is fixedly connected to the top of the twist cover, a pressing block is elastically mounted on the inner wall of the annular mounting seat, and the pressing block is in abutment with the end of the lever.

[0013] Preferably, the bottom of the hollow shaft is connected to the mixing liquid storage chamber, and a pumping chamber is provided inside the hollow shaft, a lower valve plate is hinged at the bottom of the pumping chamber, a piston ring is installed in the pumping chamber, an upper valve plate is hinged on the top surface of the piston ring, a pull rod is fixedly connected to the top of the piston ring, the top of the pull rod passes through the hollow shaft and an end cover is installed above the twist cover.

[0014] Preferably, both ends of the one-way discharge pipe are respectively connected to the liquid pumping chamber and one of the independent liquid storage chambers, and a one-way valve is installed on the one-way discharge pipe.

[0015] Preferably, the telescopic connecting pipe fitting includes a sleeve connected to an independent liquid storage cavity, one end of the sleeve is open, and an infusion tube is movably sleeved in the sleeve, a liquid inlet and a liquid outlet are respectively provided on both sides of the bottom of the infusion tube, a cross bar is fixedly connected to the top of the infusion tube, a limit pin is inserted at one end of the cross bar, and a positioning hole for inserting the limit pin is provided on the top surface of the annular culture box.

[0016] Preferably, the top end of the adjusting pressure rod is rotatably connected to a gripping rod, and a positioning rack matching the end of the gripping rod is vertically mounted on the outer wall of the hollow shaft.

[0017] In the above technical solution, a mixing liquid storage chamber and a plurality of independent liquid storage chambers are arranged in a rotatable storage box, so that the pushing and transporting mechanism can deliver the liquid in each independent liquid storage chamber into the mixing liquid storage chamber and different culture tanks, thereby realizing the rapid supply and mixing of different culture fluids. The blocking regulating member controls the opening and closing of the connecting port between the independent liquid storage chamber and the mixing liquid storage chamber, so as to accurately select various culture fluids to be mixed. Moreover, the one-way discharge pipe can prevent the liquid from flowing back, thereby ensuring that the liquid in the mixing liquid storage chamber flows unidirectionally to one of the independent liquid storage chambers, thereby facilitating the subsequent delivery of the mixed culture fluid in the independent liquid storage chamber into the culture tank through the pushing and transporting mechanism.

[0018] It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.

[0019] This application document provides an overview of various implementations or examples of the technology described in the present disclosure, and is not a comprehensive disclosure of the entire scope or all features of the disclosed technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0021] Figure 1 This is a schematic diagram of the overall structure of a microbial fermentation combined culture device of the present invention;

[0022] Figure 2 This is a cross-sectional view of the overall structure of a microbial fermentation combined culture device of the present invention;

[0023] Figure 3 For the present invention Figure 2A magnified view of the structure at A;

[0024] Figure 4 It is a schematic structural diagram of an annular culture box and a bottom plate in a microbial fermentation combined culture device of the present invention;

[0025] Figure 5 It is a structural schematic diagram of a storage box and a pushing and transporting mechanism in a microbial fermentation combined culture device of the present invention;

[0026] Figure 6 It is a partial cross-sectional view of a storage box in a microbial fermentation combined culture device of the present invention;

[0027] Figure 7 It is a structural schematic diagram of a push-transport mechanism and a blocking and regulating member in a microbial fermentation combined culture device of the present invention;

[0028] Figure 8 It is a partial cross-sectional view of a pushing and transporting mechanism in a microbial fermentation combined culture device of the present invention.

[0029] Description of reference numerals:

[0030] 1. chassis; 2. annular culture box; 201. culture chamber; 202. observation window; 203. transparent panel; 204. positioning hole; 3. culture tank; 301. injection nozzle; 4. storage box; 401. independent liquid storage chamber; 402. mixed liquid storage chamber; 403. connecting port; 404. inner air chamber; 405. outer observation chamber; 5. push and transfer mechanism; 501. piston pressure plate; 502. piston top plate; 503. piston support plate; 504. adjusting pressure rod; 505. hollow shaft rod; 506. twist cover; 507. Annular mounting seat; 508, pressure block; 509, pumping chamber; 510, lower valve plate; 511, piston ring; 512, upper valve plate; 513, pull rod; 514, end cover; 515, grip rod; 516, positioning rack; 6, plugging adjustment member; 601, toggle frame; 602, sealing plate; 603, toggle rod; 7, one-way discharge pipe; 701, one-way valve; 8, telescopic connecting pipe fitting; 801, sleeve; 802, infusion tube; 803, liquid inlet; 804, liquid discharge port; 805, cross bar; 806, limit pin. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the embodiments of the present disclosure clearer, the technical solution of the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings of the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, not all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

[0032] See also Figure 1-8 A microbial fermentation combined culture device provided by an embodiment of the present invention comprises a chassis 1, and also comprises an annular culture box 2, which is fixedly mounted on the top surface of the chassis 1, and a plurality of culture chambers 201 are opened in the annular culture box 2, and a culture tank 3 is placed in the culture chamber 201, a storage box 4, which is rotatably mounted on the top surface of the chassis 1, and the storage box 4 is surrounded by the annular culture box 2, and a plurality of independent liquid storage chambers 401 connected to each culture tank 3 are arranged on the periphery of the storage box 4, and a mixing liquid storage chamber 402 is arranged in the middle of the storage box 4, and a pushing and transporting mechanism 5 is installed in the storage box 4, and is used for sending the liquid in the independent liquid storage chamber 401 into the culture tank 3 and the mixing liquid storage chamber 402, and a connecting port 403 is arranged between the mixing liquid storage chamber 402 and each independent liquid storage chamber 401, and a plugging adjustment member 6 adapted to the connecting port 403 is installed in the pushing and transporting mechanism 5, and a one-way discharge pipe 7 is installed between one of the connecting ports 403 and the pushing and transporting mechanism 5.

[0033] Specifically, when using the combined culture device for microbial fermentation, firstly, a plurality of culture tanks 3 are placed in the culture chambers 201 of the annular culture box 2, and the required culture fluids are respectively injected into the independent liquid storage chambers 401 of the storage box 4. During the process of microbial fermentation culture, the culture fluid in the independent liquid storage chamber 401 can be delivered to the culture tank 3 by the push-transport mechanism 5. The storage box 4 can rotate independently in the annular culture box 2, and then adjust the relative positions between the independent liquid storage chambers 401 and the culture tanks 3 to provide various nutrients required for the fermentation of the microorganisms. At the same time, the push-transport mechanism 5 can also push the liquid into the mixed liquid storage chamber 402 for mixing. The function of the blocking regulating member 6 is to control The opening and closing of the connecting port 403 can control the liquid exchange between each independent liquid storage chamber 401 and the culture tank 3 and the mixing liquid storage chamber 402 by adjusting the position of the plugging adjustment member 6. As long as the plugging adjustment member 6 is lifted, the corresponding connecting port 403 will be in an open state, and then the pushing and transporting mechanism 5 is used to allow the culture fluid in the independent liquid storage chamber 401 to enter the independent mixing chamber along the opened connecting port 403 to be mixed, thereby achieving precise regulation of the flow of the culture fluid. The function of the one-way discharge pipe 7 is to ensure that the liquid in the mixing liquid storage chamber 402 can only flow in one direction to one of the independent liquid storage chambers 401 specially used for temporarily storing the mixed liquid, to prevent the liquid inside it from flowing back, and to ensure that the liquid in the mixing liquid storage chamber 402 always remains fresh.

[0034] Compared with the prior art, the embodiment of the present invention arranges a mixing liquid storage chamber 402 and multiple independent liquid storage chambers 401 in a rotatable storage box 4, so that the pushing and transporting mechanism 5 can send the liquid in each independent liquid storage chamber 401 into the mixing liquid storage chamber 402 and different culture tanks 3, thereby realizing the rapid supply and mixing of different culture fluids. The blocking adjustment member 6 controls the opening and closing of the connecting port 403 between the independent liquid storage chamber 401 and the mixing liquid storage chamber 402, so as to accurately select various culture fluids to be mixed. Moreover, the one-way discharge pipe 7 can prevent the liquid from flowing back, thereby ensuring that the liquid in the mixing liquid storage chamber 402 flows unidirectionally to one of the independent liquid storage chambers 401, thereby facilitating the subsequent delivery of the mixed culture fluid in the independent liquid storage chamber 401 into the culture tank 3 through the pushing and transporting mechanism 5.

[0035] In a further embodiment of the present invention, observation windows 202 corresponding to each culture tank 3 are provided on the outer peripheral surface of the annular culture box 2, and transparent panels 203 are installed in the observation windows 202. Specifically, through these observation windows 202, the user can conveniently observe the growth of microorganisms in the culture tank 3 without opening the culture box. The transparent panel 203 can protect the environment in the culture tank 3 from external interference without hindering observation. The transparent panel 203 allows light to pass through, and the user can observe the changes in the morphology, quantity and growth state of the microorganisms in the culture tank 3 with the naked eye or with the help of tools such as a microscope. In this way, the user can timely understand the growth of the microorganisms and adjust the culture conditions, such as rotating the storage box 4 to adjust the independent liquid storage chamber 401 connected to the culture tank 3, so as to replace different culture solutions to obtain better culture effects. At the same time, the setting of the observation window 202 also facilitates the simultaneous observation and comparison of multiple culture tanks 3, thereby improving the experimental efficiency and the accuracy of the data.

[0036] In a further embodiment of the present invention, a telescopic connecting pipe 8 is installed on the top of the independent liquid storage chamber 401, and a liquid injection nozzle 301 for inserting the telescopic connecting pipe 8 is installed on the top of the culture tank 3. Specifically, when liquid needs to be added to the culture tank 3, the entire storage box 4 is first rotated to align the independent liquid storage chamber 401 with the corresponding culture chamber 201, and then the telescopic connecting pipe 8 on the independent liquid storage chamber 401 is pulled to insert its tube head into the liquid injection nozzle 301 on the culture tank 3 to achieve the connection between the independent liquid storage chamber 401 and the culture tank 3. The telescopic connecting pipe 8 can adapt to the relative position change between the storage box 4 and the culture tank 3 to ensure that the liquid can be accurately injected into the culture tank 3, and the telescopic connecting pipe 8 can be adjusted and retracted before the storage box 4 is rotated. The setting of the liquid injection nozzle 301 can prevent liquid leakage and facilitate the insertion and removal of the telescopic connecting pipe 8. In this way, the closed addition of liquid is achieved to ensure the closed environmental conditions of the culture device.

[0037] In a further embodiment of the present invention, a plurality of inner air chambers 404 and a plurality of outer observation chambers 405 connected thereto are distributed in an annular manner above the interior of the storage box 4, and the pushing and transferring mechanism 5 includes a piston pressure plate 501 movably installed in the inner air chamber 404 and a piston top plate 502 movably installed in the outer observation chamber 405, a piston support plate 503 located in the independent liquid storage cavity 401 is installed at the bottom of the piston top plate 502, and an adjusting pressure rod 504 penetrating the top of the storage box 4 is fixedly connected to the top of the piston top plate 502. Specifically, by pressing or pulling the adjusting pressure rod 504, the piston pressure plate 501 can be driven to move up and down in the inner air chamber 404. When the piston pressure plate 501 moves downward, the air in the inner air chamber 404 is compressed, and the air is squeezed into the corresponding outer observation chamber 405 connected to the inner air chamber 404. , thereby forcing the piston top plate 502 to move upward. Since the piston top plate 502 and the piston support plate 503 in the independent liquid storage chamber 401 are connected together, when the piston support plate 503 is lifted from the bottom of the independent liquid storage chamber 401, the liquid in the independent liquid storage chamber 401 will be pushed to the telescopic connecting pipe fitting 8, and then the squeezed nutrient solution is discharged into the culture tank 3 through the telescopic connecting pipe fitting 8 inserted into the injection nozzle 301. On the contrary, the piston pressure plate 501 moves upward, the pressure in the inner air chamber 404 decreases, and the air in the outer observation chamber 405 re-enters the inner air chamber 404, causing the piston top plate 502 to move downward, making it convenient to add culture solution in the independent liquid storage chamber 401. The outer observation chamber 405 can be used to observe the pushing situation of the liquid and the remaining amount, so as to adjust the operation in time, and the operation is convenient to use.

[0038] In a further embodiment of the present invention, the push transfer mechanism 5 also includes a hollow shaft 505 fixedly inserted in the storage box 4, and a twist cover 506 is fixedly connected to the top of the hollow shaft 505. The blocking adjustment member 6 includes a plurality of toggle frames 601 vertically slidably connected to the inner side wall of the hollow shaft 505. A sealing plate 602 is installed at the bottom end of the toggle frame 601 at the connecting port 403, and a toggle rod 603 is installed at the top of the toggle frame 601 on the top surface of the twist cover 506. Specifically, when it is necessary to adjust the blocking adjustment member 6, the toggle rod 603 can be held to lift the corresponding toggle frame 601 upwards, and the hollow shaft 505 limits the rotation of the toggle frame 601, so that the toggle frame 601 can only move in the vertical direction. When moving upward, the sealing plate 602 moves upward accordingly, opening the connecting port 403 that was originally blocked by the sealing plate 602, and the mixed liquid storage chamber 402 and the corresponding independent liquid storage chamber 401 are connected to each other through the connecting port 403, so that when the piston top plate 502 moves upward to drive the piston support plate 503 to be lifted from the bottom of the independent liquid storage chamber 401, the liquid in the independent liquid storage chamber 401 is forced to be pushed through the connecting port 403 and flow into the mixed liquid storage chamber 402. Conversely, when the driving frame 601 moves downward with the sealing plate 602, the connecting port 403 is blocked to prevent the liquid from flowing out. In this way, the blocking and opening operations of each connecting port 403 can be realized, thereby meeting the mixing requirements of different nutrient solutions.

[0039] In a further embodiment of the present invention, an annular mounting seat 507 is fixedly connected to the top of the twist cover 506, and a pressure block 508 is elastically mounted on the inner wall of the annular mounting seat 507. A top spring is connected between one end of the pressure block and the annular mounting seat 507, and the other end of the pressure block 508 abuts and cooperates with the end of the lever 603. Specifically, since the pressure block 508 is elastically mounted on the inner wall of the annular mounting seat 507 and abuts and cooperates with the end of the lever 603, when the toggle frame 601 is not lifted, the pressure block 508 and the end of the lever 603 remain in contact and apply a certain pressure to keep the entire blocking adjustment member 6 stable. When the toggle frame 601 is moved up to lift the lever 603, the pressure block will automatically translate to the bottom of the lever 603 under the action of the elastic force. At this time, even if the lever 603 is released, the toggle frame 601 connected to the lever 603 will not drop, so that the corresponding connecting port 403 is always kept in an open state, making the mixing operation more stable and fast.

[0040] In a further embodiment of the present invention, the bottom of the hollow shaft 505 is connected to the mixing liquid storage chamber 402, and a pumping chamber 509 is provided inside the hollow shaft 505, and a lower valve plate 510 is hinged at the bottom of the pumping chamber 509, a piston ring 511 is installed in the pumping chamber 509, and an upper valve plate 512 is hinged on the top surface of the piston ring 511, and a pull rod 513 is fixedly connected to the top of the piston ring 511, and the top of the pull rod 513 passes through the hollow shaft 505 and an end cover 514 is installed above the twist cover 506. Specifically, when the nutrient solutions are injected into the mixing liquid storage chamber 402 for mixing, it is necessary to extract the liquid in the mixing liquid storage chamber 402, first, the end cover 514 is pulled upward, and the pull rod 513 drives the piston ring 511 to move upward, so that the mixed liquid in the mixing liquid storage chamber 402 passes through the hollow shaft 505. The bottom is sucked into the liquid extraction chamber 509, and the upper valve plate 512 is always in a closed state at this time, while the lower valve plate 510 is opened due to the impact of the water flow, and then the piston ring 511 is squeezed downward by the pull rod 513. Since the lower valve is closed again, the upper valve on the piston ring 511 is forced to open, and the mixed liquid can only gradually flow to the top of the piston ring 511. After the upper valve is closed, the pull rod 513 is pulled again to lift the piston ring 511, and the piston ring 511 is used to allow the mixed liquid in the liquid extraction chamber 509 to enter one of the independent liquid storage chambers 401 specially used for storing the mixed liquid through the one-way discharge pipe 7. By repeatedly pulling the end cover 514, the continuous extraction of the liquid in the mixed liquid storage chamber 402 can be achieved. This design has a simple structure and is easy to operate, and can effectively extract liquid for subsequent processing or use.

[0041] In a further embodiment of the present invention, both ends of the one-way discharge pipe 7 are respectively connected to the pumping chamber 509 and one of the independent liquid storage chambers 401, and a one-way valve 701 is installed on the one-way discharge pipe 7. Specifically, when the piston ring 511 moves upward, the pressure in the pumping chamber 509 decreases, and the one-way valve 701 opens, so that the liquid in the mixed liquid storage chamber 402 can enter the independent liquid storage chamber 401 through the one-way discharge pipe 7. Due to the one-way conduction characteristics of the one-way valve 701, the liquid can only flow from the pumping chamber 509 to the independent liquid storage chamber 401, and cannot flow in the opposite direction. This ensures that the liquid flows in a predetermined direction and realizes one-way discharge of the liquid. When the piston ring 511 moves downward, the pressure in the pumping chamber 509 increases, and the one-way valve 701 closes to prevent the liquid from flowing back. In this way, the directional transportation and distribution of the liquid can be achieved to meet the needs of the microbial fermentation combined culture device.

[0042] In a further embodiment of the present invention, the telescopic connecting pipe 8 includes a sleeve 801 connected to the independent liquid storage chamber 401, one end of the sleeve 801 is open, and an infusion tube 802 is movably sleeved in the sleeve 801, and a liquid inlet 803 and a liquid outlet 804 are respectively provided on both sides of the bottom of the infusion tube 802, and a cross bar 805 is fixedly connected to the top of the infusion tube 802, and a liquid inlet tube is installed between the cross bar 805 and the infusion tube 802, and a limiting pin 806 is inserted at one end of the cross bar 805, and a positioning hole 204 for inserting the limiting pin 806 is provided on the top surface of the annular culture box 2. Specifically, when it is necessary to add liquid to the culture tank 3 in the annular culture box 2, the storage box 4 is first rotated to adjust the position of the independent liquid storage chamber 401, and then the cross bar 805 is pulled toward the direction of the culture tank 3, and the limiting pin 806 on the cross bar 805 is inserted into the positioning hole 204 on the top surface of the annular culture box 2, so that the infusion tube 802 extends out of the sleeve 801 and After the position is fixed, the head part of the infusion tube 802 has been inserted into the injection nozzle 301 on the culture tank 3. At this time, the liquid in the independent liquid storage chamber 401 can enter the infusion tube 802 through the liquid inlet 803 of the infusion tube 802 when the piston support plate 503 is squeezed upward, and is discharged from the discharge port 804, and then enters the corresponding culture tank 3 in the annular culture box 2 through the injection nozzle 301. When the liquid is added, the cross bar 805 is pushed to completely insert the infusion tube 802 into the sleeve 801, so that the liquid inlet 803 leaves the injection nozzle 301, thereby stopping the addition of liquid and ensuring the sealing of the independent liquid storage chamber 401. In this way, different nutrient solutions can be easily added to each culture tank 3 in the annular culture box 2, while avoiding liquid leakage. In addition, the liquid inlet tube installed between the cross bar 805 and the infusion tube 802 can also facilitate the addition of nutrient solution to the independent liquid storage chamber 401 at any time.

[0043] In a further embodiment of the present invention, the top of the adjusting pressure rod 504 is rotatably connected to a grip rod 515, and a positioning rack 516 that cooperates with the end of the grip rod 515 is vertically installed on the outer wall of the hollow shaft 505. Specifically, the adjusting pressure rod 504 is used to facilitate the lifting and lowering of the piston pressure plate 501. When the piston pressure plate 501 descends, the nutrient solution in the independent liquid storage chamber 401 can enter the culture tank 3 and the mixed liquid storage chamber 402. Since it is often necessary to control the discharge of nutrient solutions in multiple independent liquid storage chambers 401, the positioning rack 516 can provide stable support and positioning. After the piston pressure plate 501 moves down to the corresponding height, the grip rod 515 is rotated to allow the grip rod 515 to be inserted into the positioning rack 516. This can ensure that the grip rod 515 will not shake or deviate during the process, thereby improving the accuracy and reliability of the adjustment.

[0044] The above description is only by way of illustration of certain exemplary embodiments of the present invention. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A microbial fermentation combined culture device, comprising a base plate (1), characterized in that: Also includes: An annular culture box (2) is fixedly mounted on the top surface of the base plate (1), and a plurality of culture chambers (201) are provided in the annular culture box (2), wherein culture tanks (3) are placed in the culture chambers (201); A storage box (4) is rotatably mounted on the top surface of the chassis (1), and the storage box (4) is surrounded by the annular culture box (2). The periphery of the storage box (4) is provided with a plurality of independent liquid storage chambers (401) connected to the culture tanks (3), and the middle of the storage box (4) is provided with a mixing liquid storage chamber (402); A push-transport mechanism (5) is installed in the storage box (4) and is used to deliver the liquid in the independent liquid storage chamber (401) into the culture tank (3) and the mixed liquid storage chamber (402). A connecting port (403) is provided between the mixed liquid storage chamber (402) and each independent liquid storage chamber (401). A blocking adjustment member (6) adapted to the connecting port (403) is installed in the push-transport mechanism (5), and a one-way discharge pipe (7) is installed between one of the connecting ports (403) and the push-transport mechanism (5).

2. A microbial fermentation combined culture device according to claim 1, characterized in that: An observation window (202) corresponding to each culture tank (3) is provided on the outer peripheral surface of the annular culture box (2), and a transparent panel (203) is installed in the observation window (202).

3. The microbial fermentation combined culture device according to claim 1, characterized in that: A telescopic connecting pipe (8) is installed on the top of the independent liquid storage chamber (401), and a liquid injection nozzle (301) for inserting the telescopic connecting pipe (8) is installed on the top of the culture tank (3).

4. The microbial fermentation combined culture device according to claim 1, characterized in that: A plurality of inner air chambers (404) and a plurality of outer observation chambers (405) connected thereto are distributed in an annular manner on the upper interior of the storage box (4); the pushing and transferring mechanism (5) comprises a piston pressure plate (501) movably mounted in the inner air chamber (404) and a piston top plate (502) movably mounted in the outer observation chamber (405); a piston support plate (503) located in an independent liquid storage cavity (401) is mounted at the bottom of the piston top plate (502); and an adjusting pressure rod (504) penetrating the top of the storage box (4) is fixedly connected to the top of the piston top plate (502).

5. A microbial fermentation combined culture device according to claim 4, characterized in that: The pushing and transferring mechanism (5) further comprises a hollow shaft (505) fixedly inserted in the storage box (4), the top of the hollow shaft (505) being fixedly connected with a twist cover (506), the blocking and adjusting member (6) comprising a plurality of toggle frames (601) vertically slidably connected with the inner side wall of the hollow shaft (505), the bottom end of the toggle frame (601) being provided with a sealing plate (602) at the connecting port (403), and the top end of the toggle frame (601) being provided with a toggle rod (603) at the top surface of the twist cover (506).

6. The microbial fermentation combined culture device according to claim 5, characterized in that: The top of the twist cover (506) is fixedly connected with an annular mounting seat (507), and the inner wall of the annular mounting seat (507) is elastically mounted with a pressing block (508), and the pressing block (508) is in abutment with the end of the shifting rod (603).

7. The microbial fermentation combined culture device according to claim 5, characterized in that: The bottom of the hollow shaft (505) is connected to the mixing liquid storage chamber (402), and a pumping chamber (509) is provided inside the hollow shaft (505). A lower valve plate (510) is hinged at the bottom of the pumping chamber (509), a piston ring (511) is installed in the pumping chamber (509), and an upper valve plate (512) is hinged on the top surface of the piston ring (511). A pull rod (513) is fixedly connected to the top of the piston ring (511), and the top of the pull rod (513) passes through the hollow shaft (505) and an end cover (514) is installed above the twist cover (506).

8. The microbial fermentation combined culture device according to claim 7, characterized in that: The two ends of the one-way discharge pipe (7) are respectively connected to the liquid extraction chamber (509) and one of the independent liquid storage chambers (401), and a one-way valve (701) is installed on the one-way discharge pipe (7).

9. The microbial fermentation combined culture device according to claim 3, characterized in that: The telescopic connecting pipe (8) comprises a sleeve (801) connected to the independent liquid storage chamber (401), one end of the sleeve (801) is open, and a liquid infusion tube (802) is movably sleeved in the sleeve (801), and a liquid inlet (803) and a liquid outlet (804) are respectively provided on both sides of the bottom of the liquid infusion tube (802), and a cross bar (805) is fixedly connected to the top of the liquid infusion tube (802), and a limit pin (806) is inserted into one end of the cross bar (805), and a positioning hole (204) for inserting the limit pin (806) is provided on the top surface of the annular culture box (2).

10. The microbial fermentation combined culture device according to claim 5, characterized in that: The top end of the regulating pressure rod (504) is rotatably connected to a gripping rod (515), and a positioning rack (516) matching the end of the gripping rod (515) is vertically mounted on the outer wall of the hollow shaft rod (505).

Images