A microbial fermentation combined culture device

Abstract

The present invention discloses a combined microbial fermentation culture device, which relates to the technical field of microbial culture. It includes a chassis, and also includes an annular culture box fixedly installed on the top surface of the chassis. A number of culture chambers are provided in the annular culture box, and culture tanks are placed in the culture chambers. A storage box is rotatably installed on the top surface of the chassis and is surrounded by the annular culture box. A plurality of independent liquid storage chambers connected to each culture tank are provided on the periphery of the storage box, and a mixed liquid storage chamber is provided in the middle of the storage box. A pushing and transporting mechanism is installed in the storage box and is used to send the liquid in the independent liquid storage chambers into the culture tanks and the mixed liquid storage chamber. By providing a mixed liquid storage chamber and a plurality of independent liquid storage chambers in the rotatable storage box, the pushing and transporting mechanism can send the liquid in each independent liquid storage chamber into the mixed liquid storage chamber and different culture tanks, realizing the rapid supply and mixing of different culture media.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial culture, and specifically to a combined microbial fermentation culture device. Background Art

[0002] Microbial fermentation culture refers to the process of using microorganisms to carry out metabolic reactions under suitable conditions to convert raw materials into products required by humans. This process usually occurs in a specific fermentation culture device, which provides a controlled growth environment for microorganisms, including suitable temperature, pH value, nutrients, gas composition, etc. In microbial fermentation culture, microorganisms absorb nutrients and synthesize various useful compounds, such as organic acids, alcohols, antibiotics, enzymes, etc. through specific metabolic pathways. These products have wide application values in the fields of medicine, food, chemical industry, environmental protection, etc.

[0003] The patent publication number of the existing patent application is: CN211645187U, and the publication date is October 9, 2020. The name of this patent is "A Multi-type Microbial Culture Device". This patent includes a cover body support, a sealing rubber sleeve cover, a culture box group, culture boxes, a temperature control bottom layer, a base, a heating side box, and a pressing buckle plate. The culture box group is supported and installed on the base. One side of the culture box group is abutted against the heating side box. A heat preservation partition layer is vertically installed inside the culture box group to divide the culture box group into several culture boxes. The bottom of the culture box is horizontally partitioned with a culture medium bottom layer. The temperature control bottom layer is located below the culture medium bottom layer. The cover body supports are fixedly installed on both sides of the base. The beneficial effects are as follows: The multi-type microbial culture device of the present invention can perform grouped operations on the culture boxes, regulate the environmental differences in each group of boxes, realize the multi-type culture of microorganisms in each culture medium, reduce the occupation of resources, and improve the culture efficiency.

[0004] The above application has deficiencies. Currently, the microbial culture device for medical testing mainly performs by applying culture solution to a culture dish or culture tank placed individually in a culture box. This method is direct and fast, but it can only meet the culture requirements of a single culture dish for microorganisms. Or multiple culture dishes are placed in the culture box for overall culture of the microorganisms in the multiple culture dishes. Although this method meets the requirements of batch culture, the multiple culture dishes are in the same unsealed culture box, which is prone to contamination and affects the culture results. Usually, the liquid needs to be manually added to the culture tank. When multiple liquids need to be mixed, the operation will be more cumbersome and it is easy to introduce contamination. Summary of the Invention

[0005] The purpose of the present invention is to provide a combined microbial fermentation culture device to solve the above deficiencies in the prior art.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A microbial fermentation combined culture device comprises a chassis and an annular culture box, which is fixedly mounted on the top surface of the chassis and is provided with a plurality of culture chambers in which culture tanks are placed; a storage box, which is rotatably mounted on the top surface of the chassis and is surrounded by the annular culture box; a plurality of independent liquid storage chambers connected to the culture tanks are arranged on the periphery of the storage box; a mixing liquid storage chamber is arranged in the middle of the storage box; a push-transport mechanism is installed in the storage box and is used to deliver liquid in the independent liquid storage chambers into the culture tanks and the mixing liquid storage chamber; a connecting port is arranged between the mixing liquid storage chamber and each independent liquid storage chamber; a plugging adjustment member matched with the connecting port is installed in the pushing-transport mechanism, and a one-way discharge pipe is installed between one of the connecting ports and the pushing-transport mechanism.

[0008] Preferably, an observation window corresponding to each culture tank is provided on the outer peripheral surface of the annular culture box, and a transparent panel is installed in the observation window.

[0009] Preferably, a telescopic connecting pipe is installed on the top of the independent liquid storage cavity, and a liquid injection nozzle for inserting the telescopic connecting pipe is installed on the top of the culture tank.

[0010] Preferably, a plurality of inner air chambers and a plurality of outer observation chambers connected thereto are distributed in a ring shape above the interior of the storage box, the pushing and transferring mechanism comprises a piston pressure plate movably installed in the inner air chamber and a piston top plate movably installed in the outer observation chamber, a piston support plate located in an independent liquid storage cavity is installed at the bottom of the piston top plate, and an adjusting pressure rod penetrating the top of the storage box is fixedly connected to the top of the piston pressure plate.

[0011] Preferably, the pushing and transferring mechanism also includes a hollow shaft fixedly inserted in the storage box, a twist cover is fixedly connected to the top of the hollow shaft, the blocking adjustment part includes a plurality of toggle frames vertically slidably connected to the inner side wall of the hollow shaft, a sealing plate is installed at the connecting port at the bottom end of the toggle frame, and a toggle rod is installed on the top surface of the twist cover at the top end of the toggle frame.

[0012] Preferably, an annular mounting seat is fixedly connected to the top of the twist cover, a pressing block is elastically mounted on the inner wall of the annular mounting seat, and the pressing block is in abutment with the end of the lever.

[0013] Preferably, the bottom of the hollow shaft is connected to the mixing liquid storage chamber, and a pumping chamber is provided inside the hollow shaft, a lower valve plate is hinged at the bottom of the pumping chamber, a piston ring is installed in the pumping chamber, an upper valve plate is hinged on the top surface of the piston ring, a pull rod is fixedly connected to the top of the piston ring, the top of the pull rod passes through the hollow shaft and an end cover is installed above the twist cover.

[0014] Preferably, both ends of the one-way discharge pipe are respectively communicated with the liquid extraction cavity and one of the independent liquid storage cavities, and a one-way valve is installed on the one-way discharge pipe.

[0015] Preferably, the telescopic connecting pipe fitting includes a sleeve communicated with the independent liquid storage cavity. One end of the sleeve is open, and an infusion pipe is movably sleeved in the sleeve. Liquid inlet and liquid discharge ports are respectively formed on both sides of the bottom of the infusion pipe. A cross bar is fixedly connected to the top of the infusion pipe. A limiting pin is inserted at one end of the cross bar, and a positioning hole for inserting the limiting pin is formed on the top surface of the annular culture box.

[0016] Preferably, a grip rod is rotatably connected to the top end of the adjusting pressure rod, and a positioning rack cooperating with the end of the grip rod is vertically installed on the outer wall of the hollow shaft rod.

[0017] In the above technical solution, by arranging a mixing liquid storage cavity and a plurality of independent liquid storage cavities in a rotatable storage box, the pushing and transporting mechanism can send the liquids in each independent liquid storage cavity into the mixing liquid storage cavity and different culture tanks, so as to realize the rapid supply and mixing of different culture media. The plugging and adjusting member controls the opening and closing of the communication port between the independent liquid storage cavity and the mixing liquid storage cavity, precisely selects various culture media to be mixed, and the one-way discharge pipe can prevent liquid backflow, ensuring that the liquid in the mixing liquid storage cavity flows unidirectionally into one of the independent liquid storage cavities, and then it is convenient to continue to send the mixed culture medium in this independent liquid storage cavity into the culture tank through the pushing and transporting mechanism subsequently.

[0018] It should be understood that the foregoing general description and the following detailed description are merely exemplary and explanatory and are not intended to limit the present disclosure.

[0019] This application document provides an overview of various implementations or examples of the technology described in the present disclosure, and is not a full disclosure of the entire scope or all features of the disclosed technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required to be used in the embodiments. Obviously, the drawings in the following description are only some embodiments recorded in the present invention, and those of ordinary skill in the art can also obtain other drawings according to these drawings.

[0021] Figure 1 is a schematic diagram of the overall structure of a microbial fermentation combined culture device of the present invention;

[0022] Figure 2 is a cross-sectional view of the overall structure of a microbial fermentation combined culture device of the present invention;

[0023] Figure 3 In the present invention Figure 2A magnified view of the structure at A;

[0024] Figure 4 It is a schematic structural diagram of an annular culture box and a bottom plate in a microbial fermentation combined culture device of the present invention;

[0025] Figure 5 It is a structural schematic diagram of a storage box and a pushing and transporting mechanism in a microbial fermentation combined culture device of the present invention;

[0026] Figure 6 It is a partial cross-sectional view of a storage box in a microbial fermentation combined culture device of the present invention;

[0027] Figure 7 It is a structural schematic diagram of a push-transport mechanism and a blocking and regulating member in a microbial fermentation combined culture device of the present invention;

[0028] Figure 8 It is a partial cross-sectional view of a pushing and transporting mechanism in a microbial fermentation combined culture device of the present invention.

[0029] Description of reference numerals:

[0030] 1. chassis; 2. annular culture box; 201. culture chamber; 202. observation window; 203. transparent panel; 204. positioning hole; 3. culture tank; 301. injection nozzle; 4. storage box; 401. independent liquid storage chamber; 402. mixed liquid storage chamber; 403. connecting port; 404. inner air chamber; 405. outer observation chamber; 5. push and transfer mechanism; 501. piston pressure plate; 502. piston top plate; 503. piston support plate; 504. adjusting pressure rod; 505. hollow shaft rod; 506. twist cover; 507. Annular mounting seat; 508, pressure block; 509, pumping chamber; 510, lower valve plate; 511, piston ring; 512, upper valve plate; 513, pull rod; 514, end cover; 515, grip rod; 516, positioning rack; 6, plugging adjustment member; 601, toggle frame; 602, sealing plate; 603, toggle rod; 7, one-way discharge pipe; 701, one-way valve; 8, telescopic connecting pipe fitting; 801, sleeve; 802, infusion tube; 803, liquid inlet; 804, liquid discharge port; 805, cross bar; 806, limit pin. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the embodiments of the present disclosure clearer, the technical solution of the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings of the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, not all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

[0032] Please refer to Figure 1-8 , a microbial fermentation combined culture device provided by an embodiment of the present invention includes a chassis 1, and further includes an annular culture box 2, which is fixedly installed on the top surface of the chassis 1, and a plurality of culture chambers 201 are provided in the annular culture box 2. Culture tanks 3 are placed in the culture chambers 201, a storage box 4, which is rotatably installed on the top surface of the chassis 1, and the storage box 4 is surrounded by the annular culture box 2. A plurality of independent liquid storage chambers 401 connected to each culture tank 3 are provided on the periphery of the storage box 4, and a mixed liquid storage chamber 402 is provided in the middle of the storage box 4. A pushing and transporting mechanism 5 is installed in the storage box 4 for sending the liquid in the independent liquid storage chamber 401 into the culture tank 3 and the mixed liquid storage chamber 402. A communication port 403 is provided between the mixed liquid storage chamber 402 and each independent liquid storage chamber 401. A plugging and adjusting member 6 adapted to the communication port 403 is installed in the pushing and transporting mechanism 5. A one-way discharge pipe 7 is installed between one of the communication ports 403 and the pushing and transporting mechanism 5.

[0033] Specifically, when using the microbial fermentation combined culture device, first place a plurality of culture tanks 3 in the respective culture chambers 201 of the annular culture box 2, and inject the required culture solutions into the independent liquid storage chambers 401 of the storage box 4 respectively. During the process of microbial fermentation culture, the culture solution in the independent liquid storage chamber 401 can be sent into the culture tank 3 through the pushing and transporting mechanism 5. The storage box 4 can rotate independently within the annular culture box 2, thereby adjusting the relative positions between the independent liquid storage chambers 401 and the culture tanks 3 to provide various nutrients required for the fermentation of microorganisms. At the same time, the pushing and transporting mechanism 5 can also push the liquid into the mixed liquid storage chamber 402 for mixing. The function of the plugging and adjusting member 6 is to control the opening and closing of the communication port 403. By adjusting the position of the plugging and adjusting member 6, the liquid exchange between each independent liquid storage chamber 401 and the culture tank 3 and the mixed liquid storage chamber 402 can be controlled. As long as the plugging and adjusting member 6 is lifted, the corresponding communication port 403 will be in an open state, and then the pushing and transporting mechanism 5 is used to make the culture solution in the independent liquid storage chamber 401 flow into the independent mixing chamber through the opened communication port 403 for mixing, so as to achieve precise adjustment of the flow of the culture solution. The function of the one-way discharge pipe 7 is to ensure that the liquid in the mixed liquid storage chamber 402 can only flow unidirectionally into one of the independent liquid storage chambers 401 dedicated to temporarily storing the mixed liquid, preventing the internal liquid from flowing back, and ensuring that the liquid in the mixed liquid storage chamber 402 always remains fresh.

[0034] Compared with the prior art, in the embodiment of the present invention, a mixing liquid storage cavity 402 and a plurality of independent liquid storage cavities 401 are arranged in the rotatable storage box 4, so that the pushing and transporting mechanism 5 can send the liquids in the respective independent liquid storage cavities 401 into the mixing liquid storage cavity 402 and different culture tanks 3, realizing the rapid supply and mixing of different culture media. The plugging and adjusting member 6 controls the opening and closing of the communication port 403 between the independent liquid storage cavity 401 and the mixing liquid storage cavity 402, precisely selecting various culture media to be mixed, and the one-way discharge pipe 7 can prevent liquid backflow, ensuring that the liquid in the mixing liquid storage cavity 402 flows unidirectionally into one of the independent liquid storage cavities 401, and then facilitating the subsequent feeding of the mixed culture medium in this independent liquid storage cavity 401 into the culture tank 3 by the pushing and transporting mechanism 5.

[0035] In a further embodiment of the present invention, observation windows 202 corresponding to the respective culture tanks 3 are provided on the outer peripheral surface of the annular culture box 2, and a transparent panel 203 is installed in the observation windows 202. Specifically, through these observation windows 202, the user can conveniently observe the growth of microorganisms in the culture tank 3 without opening the culture box. The transparent panel 203 can protect the environment in the culture tank 3 from external interference while not hindering observation. The transparent panel 203 allows light to pass through, and the user can observe the changes in the morphology, quantity, and growth state of the microorganisms in the culture tank 3 with the naked eye or with the aid of tools such as a microscope. In this way, the user can timely understand the growth of the microorganisms and adjust the culture conditions, such as rotating the storage box 4 to adjust the independent liquid storage cavity 401 connected to the culture tank 3 to replace different culture media for better culture effects. At the same time, the setting of the observation windows 202 also facilitates the simultaneous observation and comparison of multiple culture tanks 3, improving the experimental efficiency and the accuracy of data.

[0036] In a further embodiment of the present invention, a telescopic connecting pipe fitting 8 is installed at the top of the independent liquid storage cavity 401, and a liquid injection nozzle 301 for inserting the telescopic connecting pipe fitting 8 is installed at the top of the culture tank 3. Specifically, when adding liquid to the culture tank 3, first rotate the entire storage box 4 to align the independent liquid storage cavity 401 with the corresponding culture cavity 201, and then pull the telescopic connecting pipe fitting 8 on the independent liquid storage cavity 401 to insert its pipe head part into the liquid injection nozzle 301 on the culture tank 3, realizing the connection between the independent liquid storage cavity 401 and the culture tank 3. The telescopic connecting pipe fitting 8 can adapt to the relative position changes between the storage box 4 and the culture tank 3, ensuring that the liquid can be accurately injected into the culture tank 3 and can also retract the telescopic connecting pipe fitting 8 before rotating the storage box 4. The setting of the liquid injection nozzle 301 can prevent liquid leakage and also facilitates the insertion and extraction of the telescopic connecting pipe fitting 8. In this way, the airtight addition of liquid is realized, ensuring the closed environmental conditions of the culture device.

[0037] In a further embodiment of the present invention, a plurality of inner air chambers 404 are annularly distributed above the interior of the storage box 4, and a plurality of outer observation chambers 405 communicated therewith. The pushing and transporting mechanism 5 includes a piston pressing plate 501 movably installed in the inner air chamber 404 and a piston top plate 502 movably installed in the outer observation chamber 405. A piston support plate 503 located in the independent liquid storage chamber 401 is installed at the bottom of the piston top plate 502. The top of the piston pressing plate 501 is fixedly connected to an adjusting pressure rod 504 penetrating the top of the storage box 4. Specifically, by pressing or pulling the adjusting pressure rod 504, the piston pressing plate 501 can be driven to move up and down in the inner air chamber 404. When the piston pressing plate 501 moves downward, the air in the inner air chamber 404 is compressed and the air is squeezed into the corresponding outer observation chamber 405 connected to the inner air chamber 404, thereby forcing the piston top plate 502 to move upward. Since the piston top plate 502 and the piston support plate 503 in the independent liquid storage chamber 401 are connected together, when the piston support plate 503 is lifted from the bottom of the independent liquid storage chamber 401, the liquid in the independent liquid storage chamber 401 will be pushed to the telescopic connecting pipe fitting 8, and then the squeezed nutrient solution is discharged into the culture tank 3 through the telescopic connecting pipe fitting 8 inserted into the liquid injection nozzle 301. On the contrary, when the piston pressing plate 501 moves upward, the pressure in the inner air chamber 404 decreases, and the air in the outer observation chamber 405 re-enters the inner air chamber 404, causing the piston top plate 502 to move downward, which is convenient for adding the culture solution to the independent liquid storage chamber 401. The outer observation chamber 405 can be used to observe the liquid pushing situation and the remaining amount, so as to adjust the operation in time, and the use operation is convenient.

[0038] In a further embodiment of the present invention, the push transfer mechanism 5 also includes a hollow shaft 505 fixedly inserted in the storage box 4, and a twist cover 506 is fixedly connected to the top of the hollow shaft 505. The blocking adjustment member 6 includes a plurality of toggle frames 601 vertically slidably connected to the inner side wall of the hollow shaft 505. A sealing plate 602 is installed at the bottom end of the toggle frame 601 at the connecting port 403, and a toggle rod 603 is installed at the top of the toggle frame 601 on the top surface of the twist cover 506. Specifically, when it is necessary to adjust the blocking adjustment member 6, the toggle rod 603 can be held to lift the corresponding toggle frame 601 upwards, and the hollow shaft 505 limits the rotation of the toggle frame 601, so that the toggle frame 601 can only move in the vertical direction. When moving upward, the sealing plate 602 moves upward accordingly, opening the connecting port 403 that was originally blocked by the sealing plate 602, and the mixing liquid storage chamber 402 and the corresponding independent liquid storage chamber 401 are connected to each other through the connecting port 403, so that when the piston top plate 502 moves upward to drive the piston support plate 503 to be lifted from the bottom of the independent liquid storage chamber 401, the liquid in the independent liquid storage chamber 401 is forced to be pushed through the connecting port 403 and flow into the mixing liquid storage chamber 402. Conversely, when the driving frame 601 moves downward with the sealing plate 602, the connecting port 403 is blocked to prevent the liquid from flowing out. In this way, the blocking and opening operations of each connecting port 403 can be realized, thereby meeting the mixing requirements of different nutrient solutions.

[0039] In a further embodiment of the present invention, an annular mounting seat 507 is fixedly connected to the top of the twist cover 506, and a pressure block 508 is elastically mounted on the inner wall of the annular mounting seat 507. A top spring is connected between one end of the pressure block and the annular mounting seat 507, and the other end of the pressure block 508 abuts and cooperates with the end of the lever 603. Specifically, since the pressure block 508 is elastically mounted on the inner wall of the annular mounting seat 507 and abuts and cooperates with the end of the lever 603, when the toggle frame 601 is not lifted, the pressure block 508 and the end of the lever 603 remain in contact and apply a certain pressure to keep the entire blocking adjustment member 6 stable. When the toggle frame 601 is moved up to lift the lever 603, the pressure block will automatically translate to the bottom of the lever 603 under the action of the elastic force. At this time, even if the lever 603 is released, the toggle frame 601 connected to the lever 603 will not drop, so that the corresponding connecting port 403 is always kept in an open state, making the mixing operation more stable and fast.

[0040] In a further embodiment of the present invention, the bottom of the hollow shaft rod 505 is communicated with the mixing liquid storage cavity 402, and a liquid extraction cavity 509 is provided inside the hollow shaft rod 505. A lower valve plate 510 is hinged at the bottom of the liquid extraction cavity 509, a piston ring 511 is installed in the liquid extraction cavity 509, an upper valve plate 512 is hinged on the top surface of the piston ring 511, and a pull rod 513 is fixedly connected to the top of the piston ring 511. The top of the pull rod 513 penetrates through the hollow shaft rod 505 and an end cover 514 is installed above the torsion cover 506. Specifically, after each nutrient solution is injected into the mixing liquid storage cavity 402 for mixing, it is necessary to extract the liquid in the mixing liquid storage cavity 402. First, pull the end cover 514 upward, and the pull rod 513 drives the piston ring 511 to move upward, sucking the mixed liquid in the mixing liquid storage cavity 402 into the liquid extraction cavity 509 through the bottom of the hollow shaft rod 505. At this time, the upper valve plate 512 is always in a closed state, while the lower valve plate 510 is opened due to the impact of the water flow. Then, the piston ring 511 is squeezed downward through the pull rod 513. Since the lower valve closes again, the upper valve on the piston ring 511 is forced to open, and the mixed liquid can only gradually flow above the piston ring 511. When the upper valve closes, the pull rod 513 is pulled again to lift the piston ring 511, and the piston ring 511 is used to make the mixed liquid in the liquid extraction cavity 509 enter one of the independent liquid storage cavities 401 specifically used for storing the mixed liquid through the one-way discharge pipe 7. By repeatedly pulling the end cover 514, continuous extraction of the liquid in the mixing liquid storage cavity 402 can be achieved. This design has a simple structure and is easy to operate, and can effectively extract the liquid and perform subsequent treatment or use.

[0041] In a further embodiment of the present invention, both ends of the one-way discharge pipe 7 are respectively communicated with the liquid extraction cavity 509 and one of the independent liquid storage cavities 401, and a one-way valve 701 is installed on the one-way discharge pipe 7. Specifically, when the piston ring 511 moves upward, the pressure in the liquid extraction cavity 509 decreases, and the one-way valve 701 opens, enabling the liquid in the mixing liquid storage cavity 402 to enter the independent liquid storage cavity 401 through the one-way discharge pipe 7. Due to the one-way conduction characteristic of the one-way valve 701, the liquid can only flow from the liquid extraction cavity 509 to the independent liquid storage cavity 401 and cannot flow in the reverse direction. In this way, the liquid can be ensured to flow in a predetermined direction, realizing the one-way discharge of the liquid. When the piston ring 511 moves downward, the pressure in the liquid extraction cavity 509 increases, and the one-way valve 701 closes to prevent the liquid from flowing back. In this way, the directional transportation and distribution of the liquid can be realized to meet the requirements of the microbial fermentation combined culture device.

[0042] In a further embodiment of the present invention, the telescopic connecting pipe fitting 8 includes a sleeve 801 communicating with the independent liquid storage cavity 401. One end of the sleeve 801 is open, and an infusion tube 802 is movably sleeved inside the sleeve 801. Liquid inlet ports 803 and liquid discharge ports 804 are respectively formed on both sides of the bottom of the infusion tube 802. A cross bar 805 is fixedly connected to the top of the infusion tube 802. A liquid inlet pipe is installed between the cross bar 805 and the infusion tube 802. A limit pin 806 is inserted at one end of the cross bar 805. A positioning hole 204 for inserting the limit pin 806 is formed on the top surface of the annular culture box 2. Specifically, when it is necessary to add liquid to the culture tank 3 in the annular culture box 2, first rotate the storage box 4 to adjust the position of the independent liquid storage cavity 401, and then pull the cross bar 805 in the direction of the culture tank 3, and insert the limit pin 806 on the cross bar 805 into the positioning hole 204 on the top surface of the annular culture box 2, so that the infusion tube 802 extends out of the sleeve 801 and is fixed in position. At this time, the tube head part of the infusion tube 802 has also been inserted into the liquid injection nozzle 301 on the culture tank 3. At this time, when the liquid in the independent liquid storage cavity 401 is squeezed upward by the piston support plate 503, it can enter the infusion tube 802 through the liquid inlet port 803 of the infusion tube 802, and be discharged from the liquid discharge port 804, and then enter the corresponding culture tank 3 in the annular culture box 2 through the liquid injection nozzle 301. After adding the liquid, push the cross bar 805 to completely insert the infusion tube 802 into the sleeve 801, so that the liquid inlet port 803 leaves the liquid injection nozzle 301, thereby stopping the addition of the liquid and ensuring the seal of the independent liquid storage cavity 401. In this way, different nutrient solutions can be conveniently added to each culture tank 3 in the annular culture box 2, while avoiding liquid leakage. Moreover, the liquid inlet pipe installed between the cross bar 805 and the infusion tube 802 can also facilitate the addition of nutrient solution into the independent liquid storage cavity 401 at any time.

[0043] In a further embodiment of the present invention, a grip rod 515 is rotatably connected to the top end of the adjusting lever 504. A positioning rack 516 that cooperates with the end of the grip rod 515 is vertically installed on the outer wall of the hollow shaft rod 505. Specifically, the adjusting lever 504 is used to facilitate the lifting and lowering of the piston pressing plate 501. When the piston pressing plate 501 descends, the nutrient solution in the independent liquid storage cavity 401 can enter the culture tank 3 and the mixed liquid storage cavity 402. Since it is often necessary to control the discharge of the nutrient solution in multiple independent liquid storage cavities 401, the positioning rack 516 can provide stable support and positioning. After the piston pressing plate 501 moves down to the corresponding height, rotate the grip rod 515 to insert the grip rod 515 into the positioning rack 516, which can ensure that the grip rod 515 will not shake or shift during the process, thereby improving the accuracy and reliability of the adjustment.

[0044] Only some exemplary embodiments of the present invention have been described by way of illustration. Undoubtedly, for those of ordinary skill in the art, the described embodiments can be modified in various different ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and description are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A microbial fermentation combined culture device, comprising a chassis (1), characterized in that, Further comprising: A circular culture box (2) fixedly installed on the top surface of the chassis (1), and a plurality of culture chambers (201) are provided in the circular culture box (2), and culture tanks (3) are placed in the culture chambers (201); A storage box (4) rotatably installed on the top surface of the chassis (1), and the storage box (4) is surrounded by the circular culture box (2). A plurality of independent liquid storage chambers (401) connected to each culture tank (3) are provided on the periphery of the storage box (4), and a mixed liquid storage chamber (402) is provided in the middle of the storage box (4); A telescopic connecting pipe fitting (8) is installed at the top of the independent liquid storage chamber (401), and a liquid injection nozzle (301) for inserting the telescopic connecting pipe fitting (8) is installed at the top of the culture tank (3). Pull the telescopic connecting pipe fitting (8) on the independent liquid storage chamber (401) to insert the pipe head part into the liquid injection nozzle (301) on the culture tank (3) to realize the connection between the independent liquid storage chamber (401) and the culture tank (3); A pushing and transporting mechanism (5) installed in the storage box (4) for sending the liquid in the independent liquid storage chamber (401) into the culture tank (3) and the mixed liquid storage chamber (402). A communication port (403) is provided between the mixed liquid storage chamber (402) and each independent liquid storage chamber (401). A plugging and adjusting part (6) adapted to the communication port (403) is installed in the pushing and transporting mechanism (5), and the plugging and adjusting part (6) controls the opening and closing of the communication port (403) between the independent liquid storage chamber (401) and the mixed liquid storage chamber (402). A one-way discharge pipe (7) is installed between one of the communication ports (403) and the pushing and transporting mechanism (5), and the piston support plate (503) lifts from the bottom of the independent liquid storage chamber (401) to push the liquid therein; The pushing and transporting mechanism (5) further includes a hollow shaft rod (505) fixedly inserted into the storage box (4). The bottom of the hollow shaft rod (505) is communicated with the mixed liquid storage chamber (402), and a liquid pumping chamber (509) is provided inside the hollow shaft rod (505). Both ends of the one-way discharge pipe (7) are respectively communicated with the liquid pumping chamber (509) and one of the independent liquid storage chambers (401).

2. The microbial fermentation combined culture device according to claim 1, wherein, Observation windows (202) corresponding to each culture tank (3) are provided on the outer peripheral surface of the circular culture box (2), and transparent panels (203) are installed in the observation windows (202).

3. The microbial fermentation combined culture device according to claim 1, characterized in that, A plurality of inner air chambers (404) and a plurality of outer observation chambers (405) connected thereto are distributed in an annular manner on the upper part of the storage box (4); the pushing and transferring mechanism (5) comprises a piston pressure plate (501) movably mounted in the inner air chamber (404) and a piston top plate (502) movably mounted in the outer observation chamber (405); a piston support plate (503) located in an independent liquid storage cavity (401) is mounted at the bottom of the piston top plate (502); an adjusting pressure rod (504) penetrating the top of the storage box (4) is fixedly connected to the top of the piston pressure plate (501); by pressing or pulling the adjusting pressure rod (504), the piston pressure plate (501) is driven to move up and down in the inner air chamber (404); when the piston pressure plate (501) moves downward, the air in the inner air chamber (404) is compressed and squeezed into the corresponding outer observation chamber (405) connected to the inner air chamber (404), thereby forcing the piston top plate (502) to move upward.

4. A microbial fermentation combined culture device according to claim 1, characterized in that, The top of the hollow shaft (505) is fixedly connected to a twist cover (506), and the blocking adjustment member (6) comprises a plurality of toggle frames (601) vertically slidably connected to the inner wall of the hollow shaft (505), a sealing plate (602) is installed at the bottom end of the toggle frame (601) at the connecting port (403), and a toggle rod (603) is installed at the top surface of the twist cover (506) of the top end of the toggle frame (601).

5. The microbial fermentation combined culture device according to claim 4, characterized in that, The top of the twist cover (506) is fixedly connected to an annular mounting seat (507), and an abutment block (508) is elastically mounted on the inner wall of the annular mounting seat (507), and the abutment block (508) is abutted and matched with the end of the shifting rod (603).

6. The microbial fermentation combined culture device according to claim 1, characterized in that, A lower valve plate (510) is hingedly connected to the bottom of the pumping chamber (509), a piston ring (511) is installed in the pumping chamber (509), an upper valve plate (512) is hingedly connected to the top surface of the piston ring (511), a pull rod (513) is fixedly connected to the top of the piston ring (511), the top of the pull rod (513) passes through the hollow shaft (505) and an end cover (514) is installed above the twist cover (506).

7. A microbial fermentation combined culture device according to claim 1, characterized in that, A one-way valve (701) is installed on the one-way discharge pipe (7).

8. A microbial fermentation combined culture device according to claim 1, characterized in that, The telescopic connecting pipe (8) comprises a sleeve (801) connected to the independent liquid storage chamber (401), one end of the sleeve (801) is open, and a liquid infusion tube (802) is movably sleeved in the sleeve (801), a liquid inlet (803) and a liquid outlet (804) are respectively provided on both sides of the bottom of the liquid infusion tube (802), a cross bar (805) is fixedly connected to the top of the liquid infusion tube (802), a limit pin (806) is inserted at one end of the cross bar (805), and a positioning hole (204) for inserting the limit pin (806) is provided on the top surface of the annular culture box (2).

9. The microbial fermentation combined culture device according to claim 3, wherein, The top end of the regulating pressure rod (504) is rotatably connected to a gripping rod (515), and a positioning rack (516) matching the end of the gripping rod (515) is vertically mounted on the outer wall of the hollow shaft rod (505).

Images