A method and device for preparing eggshell membrane extract containing aminoglucose component

Abstract

The invention discloses a method and device for preparing an eggshell membrane extract containing glucosamine. The preparation device includes an outer tank body, an inner tank body, a rotary drive device, an exhaust port, a finished product discharge port, a filter residue discharge pipe, a pressurized air pipe, a raw material feed pipe, a filtrate feed pipe, an atomizing nozzle, an inner air jet, an outer air jet, and an air supply system. The preparation method includes shell membrane rehydration, adding a reducing agent, adjusting pH value, enzymolysis, enzyme inactivation, activated carbon insulation, filtration, low-temperature spray drying, and feeding. The preparation device integrates the eggshell membrane hydrolyzate extraction equipment into one, reduces the space occupied by the equipment, and improves production efficiency; by driving the atomizing nozzle to circulate in the annular cavity, the annular cavity is evenly sprayed; the low-temperature dry air sprayed by the inner air jet and the outer air jet fully contacts the atomized filtrate sprayed by the atomizing nozzle, forming local turbulence, improving the contact effect between the atomized filtrate and the dry gas, and improving the drying efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of egg processing and utilization, in particular to a method and a device for preparing an eggshell membrane extract containing a glucosamine component. Background Art

[0002] The eggshell membrane is a thin film about 65 microns thick between the egg white and the eggshell. Its main components are glycoproteins, accounting for about 90%, including collagen, elastin, keratin, hyaluronic acid sulfate cartilage and glucosamine. Glucosamine has important application value and can be used to treat osteoporosis and arthritis. In addition, it also has certain antibacterial and anti-inflammatory capabilities, which plays a very important role in maintaining human health.

[0003] Protein can only fully exert its efficacy in a dissolved state. The patent with the announcement number CN114085880B discloses a method for extracting eggshell membrane hydrolysate and a suction filtration device for extraction. The eggshell membrane hydrolysate is obtained by rehydrating the eggshell membrane, adding a reducing agent, adjusting the pH value, enzymatic hydrolysis, adding alkaline protease, inactivating the enzyme, adding activated carbon, heat preservation and stirring, suction filtration, and low-temperature spray drying. Adding activated carbon can remove the odor and impurities generated during the reaction, but it is also necessary to filter the reaction system through a suction filtration device to remove the activated carbon and collect the filtrate obtained after filtration.

[0004] The eggshell membrane hydrolyzate extracted by the above-mentioned eggshell membrane hydrolyzate extraction method is the same as the eggshell membrane extract containing glucosamine components in the present application. The above-mentioned eggshell membrane hydrolyzate extraction method requires the use of a variety of equipment, including at least a container with a stirring function, a filtration device and a low-temperature spray drying device. The equipment occupies a large space, the flow of materials requires manual operation, and the degree of automation is low.

[0005] In addition, the spray drying equipment of the prior art, such as the patent with publication number CN116712742B, discloses an industrial open spray multi-stage drying system, in which the spray gun is located directly above the inside of the drying tower, the main air intake is preheated by the fresh air preheater, and then enters the drying tower through the main air intake heat exchanger and the main air intake high efficiency filter in sequence. The fluid sprayed by the spray gun of the above industrial open spray multi-stage drying system has poor fluidity in the drying tower. When the air intake temperature is low, since the spray drying technology is greatly affected by the gas temperature, the air intake temperature of the low-temperature spray drying equipment is low, and the heat transfer coefficient will also become lower, resulting in lower thermal efficiency. Since the nozzle is located in the center, the sprayed atomized material diffuses from the center to the surroundings in the drying tower, which easily leads to a higher material concentration in the central area of ​​the drying tower, relatively sufficient contact with the hot air, and a faster drying speed; while the area close to the tower wall has relatively less material and a slower drying speed, resulting in uneven drying and affecting the drying effect. Summary of the invention

[0006] Based on this, it is necessary to provide a method and device for preparing an eggshell membrane extract containing glucosamine components in order to solve the above technical problems.

[0007] In order to achieve the above-mentioned purpose, on the one hand, the present invention provides a preparation device of eggshell membrane extract containing glucosamine component, comprising an outer tank body, an inner tank body, and a rotating drive device, wherein the top of the outer tank body is provided with an exhaust port, the bottom of the outer tank body is provided with a finished product discharge port, the finished product discharge port is provided with a finished product discharge valve, the inner tank body is fixed in the outer tank body, a filter residue discharge pipe is provided at the bottom of the inner tank body, a filter residue discharge valve is installed on the upper part of the filter residue discharge pipe, a pressurized air pipe is connected to the filter residue discharge pipe and above the filter residue discharge valve, a one-way valve is installed on the pressurized air pipe near the filter residue discharge pipe, the lower end of the filter residue discharge pipe extends out of the outer tank body, an annular cavity is formed between the inner tank body and the outer tank body, a raw material feed pipe is rotatably and sealably installed on the outer tank body, a feed valve is installed on the upper part of the raw material feed pipe, the lower end of the raw material feed pipe extends into the inner tank body and is rotatably and sealably connected to the inner tank body, and a filter is provided in the inner tank body. A liquid feeding pipe, a filtrate feeding pipe and a raw material feeding pipe are fixedly connected, the upper end of the filtrate feeding pipe extends from the upper part of the inner tank body to the raw material feeding pipe and is equipped with an atomizing nozzle, and the rotary driving device drives the raw material feeding pipe to rotate so that the atomizing nozzle rotates in the annular cavity; a stirring blade and a filter cartridge are fixed on the lower end of the filtrate feeding pipe from top to bottom in sequence, and the filter cartridge is connected to the raw material feeding pipe; the inner tank body is provided with an inner interlayer, and a plurality of inner jet nozzles are installed in the inner interlayer, and the output end of the inner jet nozzle is located in the annular cavity, and a plurality of outer jet nozzles are installed on the outer wall of the outer tank body, and the output end of the outer jet nozzle is located in the annular cavity, the booster air pipe, the inner jet nozzle and the outer jet nozzle are all connected with the air supply system, and the angle between the tangent of the intersection point of the axis of the inner jet nozzle and the turnover path of the atomizing nozzle and the axis of the inner jet nozzle is less than 90 degrees, and the angle between the tangent of the intersection point of the axis of the outer jet nozzle and the turnover path of the atomizing nozzle and the axis of the outer jet nozzle is less than 90 degrees.

[0008] Preferably, the outer jet heads installed on the outer wall of the outer tank body are arranged in multiple layers, and the outer jet heads of each layer are distributed circumferentially on the outer wall of the outer tank body; the inner jet heads installed in the inner interlayer are arranged in multiple layers, and the inner jet heads of each layer are distributed circumferentially in the inner interlayer.

[0009] Preferably, an air inlet sleeve is fixed on the outer wall of the outer tank body, an outer interlayer is formed between the air inlet sleeve and the outer tank body, and the outer air jet heads are all located in the outer interlayer.

[0010] Preferably, a partition is fixed in the inner interlayer, the partition divides the inner interlayer into an upper interlayer and a lower interlayer, the inner jet head is located in the upper interlayer, and an electric heater is installed in the lower interlayer.

[0011] Preferably, the air supply system includes a dehumidifier, a main air supply pipe, a dry air supply pipe, a gas boosting device, an external air supply pipe and an internal air supply pipe. The main air supply pipe is installed at the output end of the dehumidifier, and the gas boosting device is installed on the main air supply pipe. The output end of the main air supply pipe is connected to the boosting air pipe and the dry air supply pipe. The dry air supply pipe is connected to the external air supply pipe and the internal air supply pipe. The external air supply pipe is connected to the external interlayer, and the internal air supply pipe is connected to the upper interlayer. The dry air supply pipe is installed with a first air heater, a first regulating valve, a first temperature sensor, and a first air pressure sensor; the boosting air pipe is installed with a second air heater, a second regulating valve, a second temperature sensor, and a second air pressure sensor.

[0012] Preferably, a third temperature sensor and a liquid level meter are installed in the inner tank body, a heat dissipation port is provided at the top of the inner tank body, and a heat dissipation valve is installed on the heat dissipation port.

[0013] Preferably, the exhaust port is connected to a cyclone dust collector via an exhaust pipe, an exhaust valve and a fourth temperature sensor are installed on the exhaust pipe, a storage box is connected to the bottom of the cyclone dust collector, a discharge port is provided at the bottom of the storage box, and a discharge valve is installed on the discharge port.

[0014] Preferably, a protective shell is installed on the top of the outer tank body, the rotary drive device is fixedly installed on the protective shell, the output end of the rotary drive device extends into the protective shell and is installed with a driving pulley, the raw material feed pipe is rotatably connected to the protective shell through a bearing, the raw material feed pipe passes through the protective shell, and a driven pulley is installed on the part of the raw material feed pipe located in the protective shell, and the driving pulley and the driven pulley are connected by a transmission belt.

[0015] Preferably, the filter cartridge includes a conical portion and a cylindrical portion, the lower end of the conical portion adjoins the upper end of the cylindrical portion, the diameter of the upper end of the conical portion is larger than the diameter of the cylindrical portion, and the cylindrical portion is located in the filter residue discharge pipe and above the filter residue discharge valve.

[0016] On the other hand, the present invention also provides a method for preparing an eggshell membrane extract containing glucosamine using the above-mentioned device for preparing an eggshell membrane extract containing glucosamine, comprising the following steps: Step 1: rehydrating the eggshell membrane; taking dried eggshell membrane and adding it into the inner tank through the raw material feed pipe according to the weight ratio of eggshell membrane to purified water of 1:20-50; Step 2, adding a reducing agent; adding the reducing agent to the inner tank through the raw material feed pipe in a final concentration of 5-20% (w / v), driving the stirring blade to rotate through the rotary drive device to promote the dissolution of the reducing agent, wherein the reducing agent is ferrous sulfide or sodium sulfite; Step 3: Adjust the pH value of the reaction system in the inner tank to 6.5-7.5; Step 4: Enzyme hydrolysis: adding alkaline protease to the inner tank through the raw material feed pipe at a final concentration of 0.5-3% (w / v), the enzymatic hydrolysis temperature is 45-70°C, and the reaction time is 12-36 hours; Step 5: Inactivate the enzyme; use high temperature to inactivate the enzyme, raise the temperature of the reaction system to 80-100 degrees Celsius, and maintain for 10-20 minutes; at this time, there is no obvious solid flaky shell membrane in the reaction system, and the reaction system is dark yellow; Step 6: Activated carbon insulation; 6.1 Rapidly cool the reaction system to 40-60 degrees Celsius; 6.2 Add activated carbon to the inner tank through the raw material feed pipe at a final concentration of 0.5~5% (w / v); 6.3 The stirring blade is driven by a rotary drive device to fully mix the activated carbon with the reaction system; the activated carbon can remove the odor and impurities generated during the reaction; Step seven, filtering and low-temperature spray drying; the air supply system ventilates the inner tank body through the booster air pipe, so that the internal pressure of the inner tank body is increased, and the filtrate entering the filter cartridge is pressed into the filtrate feeding pipe and the atomizing nozzle, and the atomizing nozzle is driven by the rotary drive device to rotate in the annular cavity, and the atomizing nozzle sprays into the annular cavity; the air supply system supplies air to the inner nozzle and the outer nozzle, and the low-temperature dry air sprayed by the inner nozzle and the outer nozzle contacts the atomized filtrate sprayed by the atomizing nozzle, so that the water evaporates rapidly and is discharged from the exhaust port, thereby obtaining an eggshell membrane extract containing glucosamine components; Step eight, unloading; open the finished product unloading valve to discharge the eggshell membrane extract containing glucosamine components in the outer tank from the finished product discharge port; open the filter residue unloading valve to discharge the filter residue in the inner tank from the filter residue discharge pipe.

[0017] Compared with the prior art, the technical solution has at least one of the following beneficial effects: integrating the eggshell membrane hydrolyzate extraction equipment into one, reducing the space occupied by the equipment, reducing the manpower burden, improving the degree of production automation, and improving production efficiency; By opening the heat dissipation valve, the pressurized air pipe passes unheated dry air into the inner tank body, and after the dry air exchanges heat with the reaction system in the inner tank body, it is discharged from the heat dissipation port to cool down the reaction system; the unheated dry air is passed into the outer interlayer through the external air supply pipe, and then sprayed out from the external spray head, and the internal air supply pipe passes the unheated dry air into the upper interlayer, and then sprayed out from the internal spray head, to dissipate heat to the annular cavity, thereby dissipating heat to the inner tank body, and realizing rapid cooling of the reaction system; The inner tank body is ventilated through the air supply system, so that the internal pressure of the inner tank body is increased, and the filtrate entering the filter cartridge is pressed into the filtrate feeding pipe and the atomizing nozzle. The high-pressure filtrate and high-pressure air enter the atomizing nozzle. The filtrate feeding pipe and the atomizing nozzle are driven by the rotary drive device to rotate in the annular cavity, so that the atomizing nozzle sprays evenly into the annular cavity; at the same time, the air supply system supplies air to the inner and outer nozzles, and the low-temperature dry air sprayed by the inner and outer nozzles fully contacts with the atomized filtrate sprayed by the atomizing nozzle, forming local turbulence, thereby improving the contact effect between the atomized filtrate and the dry gas, improving the drying efficiency, and avoiding the situation where the drying effect is affected by uneven drying. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic structural diagram of a device for preparing an eggshell membrane extract containing glucosamine components according to Example 1; Figure 2 It is a partial schematic diagram of the device for preparing the eggshell membrane extract containing glucosamine component of Example 1; Figure 3 for Figure 2 Sectional view along line AA; In the figure, 1, outer tank body; 2, inner tank body; 3, rotary drive device; 4, exhaust port; 5, finished product discharge port; 6, finished product discharge valve; 7, filter residue discharge pipe; 8, filter residue discharge valve; 9, protective cover; 10, booster air pipe; 11, one-way valve; 12, annular cavity; 13, raw material feed pipe; 14, feed valve; 15, filtrate feed pipe; 16, atomizing nozzle; 17, stirring blade; 18, filter cartridge; 19, inner interlayer; 20, inner jet nozzle; 21, outer jet nozzle; 22, air intake sleeve; 23, outer interlayer; 24, partition; 25, electric heater; 26, dehumidifier; 27, main air supply pipe; 28, dry air supply pipe; 29, gas booster device; 30, external air supply pipe; 31, Inner air supply pipe; 32. first air heater; 33. first regulating valve; 34. first temperature sensor; 35. first air pressure sensor; 36. second air heater; 37. second regulating valve; 38. second temperature sensor; 39. second air pressure sensor; 40. third temperature sensor; 41. liquid level gauge; 42. heat dissipation port; 43. heat dissipation valve; 44. exhaust pipe; 45. cyclone dust collector; 46. exhaust valve; 47. fourth temperature sensor; 48. storage box; 49. discharge port; 50. discharge valve; 51. protective shell; 52. driving pulley; 53. driven pulley; 54. transmission belt; 55. conical cylinder; 56. cylindrical cylinder; 57. pneumatic vibrator. DETAILED DESCRIPTION

[0019] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below. Example 1

[0020] See also Figures 1 to 3 The present application embodiment provides a preparation device for an eggshell membrane extract containing a glucosamine component, comprising an outer tank body 1, an inner tank body 2, and a rotary drive device 3. The outer tank body 1 is provided with an exhaust port 4 at the top, and a finished product discharge port 5 at the bottom of the outer tank body 1. A finished product discharge valve 6 is installed on the finished product discharge port 5. The finished product discharge valve 6 is a manual butterfly valve; An inner tank body 2 is fixed inside the outer tank body 1, a filter residue discharge pipe 7 is provided at the bottom of the inner tank body 2, a filter residue discharge valve 8 is installed on the upper part of the filter residue discharge pipe 7, the filter residue discharge valve 8 uses an electric butterfly valve or a pneumatic butterfly valve, a protective cover 9 is fixed at the bottom of the inner tank body 2, and the filter residue discharge valve 8 is arranged in the protective cover 9, and the protective cover 9 can protect the filter residue discharge valve 8 to prevent the material from contacting it; A pressurized air pipe 10 is connected to the residue discharge pipe 7 and is located above the residue discharge valve 8. A one-way valve 11 is installed on the pressurized air pipe 10 near the residue discharge pipe 7. The one-way valve 11 can prevent the material in the residue discharge pipe 7 from flowing back to the pressurized air pipe 10. The lower end of the residue discharge pipe 7 extends out of the outer tank body 1, and an annular cavity 12 is formed between the inner tank body 2 and the outer tank body 1. A raw material feed pipe 13 is installed on the outer tank body 1 in a rotating seal. The outer tank body 1 is connected to the outer tank body 1 through a mechanical seal. The raw material feed pipe 13 is rotatably sealed and connected to the inner tank body 2. A feed valve 14 is installed on the upper part of the raw material feed pipe 13. The feed valve 14 is a manual butterfly valve. The lower end of the raw material feed pipe 13 extends into the inner tank body 2 and is rotatably sealed and connected to the inner tank body 2. The raw material feed pipe 13 is rotatably sealed and connected to the inner tank body 2 through another mechanical seal. A filtrate feed pipe 15 is provided in the inner tank body 2. The filtrate feed pipe 15 is fixedly connected to the raw material feed pipe 13. The upper end of the filtrate feed pipe 15 extends from the upper part of the inner tank body 2 to the inner tank body 2. The raw material feed pipe 13 is extended and is equipped with an atomizing nozzle 16. The rotary drive device 3 drives the raw material feed pipe 13 to rotate so that the atomizing nozzle 16 rotates in the annular cavity 12; the lower end of the filtrate feeding pipe 15 is fixed with a stirring blade 17 and a filter cartridge 18 from top to bottom, and the filter cartridge 18 is connected to the raw material feed pipe 13; the inner tank body 2 is provided with an inner interlayer 19, and a plurality of inner jet heads 20 are installed in the inner interlayer 19. The output end of the inner jet head 20 is located in the annular cavity 12, and the outer tank body A plurality of outer nozzles 21 are installed on the outer wall, and the output end of the outer nozzle 21 is located in the annular cavity 12. The pressurized air pipe 10, the inner nozzle 20 and the outer nozzle 21 are all connected with the air supply system. The angle between the tangent line of the intersection point between the axis of the inner nozzle 20 and the turnover path of the atomizing nozzle 16 and the axis of the inner nozzle 20 is less than 90 degrees, and the angle between the tangent line of the intersection point between the axis of the outer nozzle 21 and the turnover path of the atomizing nozzle 16 and the axis of the outer nozzle 21 is less than 90 degrees.

[0021] The outer spray heads 21 installed on the outer wall of the outer tank body 1 are arranged in multiple layers, and the outer spray heads 21 of each layer are circumferentially distributed on the outer wall of the outer tank body 1; the inner spray heads 20 installed in the inner interlayer 19 are arranged in multiple layers, and the inner spray heads 20 of each layer are circumferentially distributed in the inner interlayer 19. The inner spray heads 20 and the drying gas sprayed from the inner spray heads 20 will impact the atomizing nozzle 16 to spray the atomized filtrate, forming local turbulence, improving the contact effect between the atomized filtrate and the drying gas, and improving the drying efficiency.

[0022] In order to facilitate the external jet heads 21 to connect to the air supply system, an air inlet sleeve 22 is fixed on the outer wall of the outer tank body 1, and an outer interlayer 23 is formed between the air inlet sleeve 22 and the outer tank body 1, and the external jet heads 21 are all located in the outer interlayer 23. The air supply system supplies air to the outer interlayer 23, thereby supplying air to each external jet head 21.

[0023] In order to facilitate the inner jet head 20 to be connected to the air supply system and to facilitate heating and heat preservation of the inner tank body 2, a partition 24 is fixed in the inner interlayer 19, and the partition 24 divides the inner interlayer 19 into an upper interlayer and a lower interlayer. The inner jet head 20 is located in the upper interlayer, and an electric heater 25 is installed in the lower interlayer. By providing the partition 24, the electric heater 25 can be prevented from contacting the air transported by the air supply system, and the electric heater 25 can be prevented from heating the air transported by the air supply system and affecting the air supply temperature.

[0024] The air supply system includes a dehumidifier 26, a main air supply pipe 27, a dry air supply pipe 28, a gas booster 29, an external air supply pipe 30 and an internal air supply pipe 31. The main air supply pipe 27 is installed at the output end of the dehumidifier 26, and the gas booster 29 is installed on the main air supply pipe 27. The output end of the main air supply pipe 27 is connected to the boosting air pipe 10 and the dry air supply pipe 28. The dry air supply pipe 28 is connected to the external air supply pipe 30 and the internal air supply pipe 31. The external air supply pipe 30 is connected to the outer interlayer 23, and the internal air supply pipe 31 is connected to the upper interlayer of the inner interlayer 19. The dry air supply pipe 28 is installed with a first air heater 32, a first regulating valve 33, a first temperature sensor 34, and a first air pressure sensor 35; the boosting air pipe 10 is installed with a second air heater 36, a second regulating valve 37, a second temperature sensor 38, and a second air pressure sensor 39. The dehumidifier 26 is a conventional rotary dehumidifier 26, which is mainly composed of a dehumidification rotor, a fan, a condenser, an evaporator and other components, and can continuously supply dry air to the main air supply pipe 27. The gas booster 29 uses a high-pressure fan to increase the pressure of the dry air supplied by the main air supply pipe 27.

[0025] The dehumidifier 26 transports dry air to the main air supply pipe 27, the main air supply pipe 27 passes the dry air into the boost air pipe 10 and the dry air supply pipe 28, the dry air supply pipe 28 passes the dry air into the external air supply pipe 30 and the internal air supply pipe 31, the external air supply pipe 30 passes the dry air into the external interlayer 23, and then is sprayed out by the external nozzle 21, the internal air supply pipe 31 passes the dry air into the upper interlayer, and then is sprayed out by the internal nozzle 20; the boost air pipe 10 passes the dry air into the inner tank body 2.

[0026] The first air heater 32 can heat the air delivered by the drying air supply pipe 28, thereby controlling the temperature of the air sprayed from the outer nozzle 21 and the inner nozzle 20, and controlling the air inlet temperature of the drying air supply pipe 28 to be 40-90°C. The second air heater 36 can heat the air delivered by the booster air pipe 10 to prevent the air temperature entering the inner tank body 2 from being too low, which causes the temperature of the filtrate in the inner tank body 2 to decrease, and ensure that the temperature of the filtrate sprayed from the atomizing nozzle 16 is not too low, thereby improving the spray drying efficiency. The first temperature sensor 34 and the second temperature sensor 38 respectively detect the temperature of the air delivered in the drying air supply pipe 28 and the booster air pipe 10.

[0027] The first regulating valve 33 can adjust the air pressure of the drying air supply pipe 28, and the second regulating valve 37 can adjust the air pressure of the boosting air pipe 10. The first regulating valve 33 cooperates with the second regulating valve 37 to make the air pressure in the boosting air pipe 10 greater than the air pressure in the drying air supply pipe 28, so as to ensure that the boosting air pipe 10 can keep the inner tank body 2 at a relatively high air pressure, and ensure that the filtrate in the inner tank body 2 can be pressed out to the atomizing nozzle 16 through the filtrate feeding pipe 15. The first air pressure sensor 35 and the second air pressure sensor 39 detect the air pressure in the drying air supply pipe 28 and the boosting air pipe 10 respectively.

[0028] In order to facilitate the detection of the temperature and liquid level of the reaction system in the inner tank body 2, a third temperature sensor 40 and a liquid level gauge 41 are installed in the inner tank body 2. The third temperature sensor 40 detects the temperature of the reaction system in the inner tank body 2, and the liquid level gauge 41 detects the liquid level of the reaction system in the inner tank body 2. In order to facilitate the heat dissipation of the inner tank body 2, a heat dissipation port 42 is provided at the top of the inner tank body 2, and a heat dissipation valve 43 is installed on the heat dissipation port 42. The heat dissipation valve 43 uses a solenoid valve or an electric valve. After the heat dissipation valve 43 is opened, the heat in the inner tank body 2 can be discharged from the heat dissipation port 42.

[0029] In order to facilitate the collection of small-particle eggshell membrane extract carried in the gas discharged from the exhaust port 4, the exhaust port 4 is connected to a cyclone dust collector 45 through an exhaust pipe 44, and an exhaust valve 46 and a fourth temperature sensor 47 are installed on the exhaust pipe 44. The fourth temperature sensor 47 can detect the exhaust temperature; the bottom of the cyclone dust collector 45 is connected to a storage box 48, and a discharge port 49 is provided at the bottom of the storage box 48. A discharge valve 50 is installed on the discharge port 49. The exhaust valve 46 can be a manual valve, or an electric valve or a pneumatic valve. The discharge valve 50 can be a manual valve, or an electric valve or a pneumatic valve. The gas discharged from the exhaust port 4 enters the cyclone dust collector 45 through the exhaust pipe 44, so that the particle-containing airflow rotates, and the particles are separated from the airflow by centrifugal force and captured on the wall of the device, and then the particles fall into the storage box 48 by gravity. The discharge valve 50 is opened, and the small-particle eggshell membrane extract collected in the storage box 48 can be discharged from the discharge port 49. The exhaust end at the top of the cyclone dust collector 45 can also be connected to a bag dust collector to further collect the small-particle eggshell membrane extract that has not entered the storage box 48.

[0030] In order to facilitate the rotation drive device 3 to drive the raw material feed pipe 13 to rotate, a protective shell 51 is installed on the top of the outer tank body 1, the rotation drive device 3 is fixedly installed on the protective shell 51, the output end of the rotation drive device 3 extends into the protective shell 51 and is installed with a driving pulley 52, the raw material feed pipe 13 is rotatably connected with the protective shell 51 through a bearing, the raw material feed pipe 13 passes through the protective shell 51, and a driven pulley 53 is installed on the part of the raw material feed pipe 13 located in the protective shell 51, and the driving pulley 52 and the driven pulley 53 are connected by a transmission belt 54. By setting the bearing, the protective shell 51 bears the gravity of the raw material feed pipe 13, so that the raw material feed pipe 13 maintains a stable rotation connection with the inner tank body 2. The rotation drive device 3 uses a variable frequency motor, the rotation drive device 3 drives the driving pulley 52 to rotate, and the driving pulley 52 drives the driven pulley 53 and the raw material feed pipe 13 to rotate through the transmission belt 54.

[0031] In order to facilitate the filtrate to enter the filter cartridge 18, the filter cartridge 18 is provided with a conical cylinder portion 55 and a cylindrical portion 56. The lower end of the conical cylinder portion 55 borders the upper end of the cylindrical portion 56. The upper end diameter of the conical cylinder portion 55 is larger than the diameter of the cylindrical portion 56. The cylindrical portion 56 is located in the filter residue discharge pipe 7 and above the filter residue discharge valve 8. The conical cylinder portion 55 is provided to facilitate the entry of the filtrate and reduce the adhesion of activated carbon particles; by providing the cylindrical portion 56, it is easy to insert into the filter residue discharge pipe 7, fully press out the filtrate at the low point liquid level, and reduce the filtrate residue.

[0032] In some embodiments, based on the above-mentioned arrangement orientation of the inner nozzle 20 and the outer nozzle 21, the inner nozzle 20 and the outer nozzle 21 can also be arranged slightly tilted upward, and the output ends of the inner nozzle 20 and the outer nozzle 21 are tilted upward, and the angle is 0 to 10 degrees. The ejected gas can push the atomized filtrate upward, slow down the falling speed of the atomized filtrate, increase the residence time of the atomized filtrate in the air, and improve the drying effect.

[0033] In some embodiments, in order to facilitate the eggshell membrane extract particles to be concentrated at the finished product outlet 5, as shown in FIG. Figure 1 and Figure 2 As shown, a pneumatic vibrator 57 may also be installed at the bottom of the outer tank body 1. When the pneumatic vibrator 57 is working, it vibrates the outer tank body 1 to promote the eggshell membrane extract particles on the inner wall of the outer tank body 1 to fall to the finished product discharge port 5. Example 2

[0034] Using the device for preparing an eggshell membrane extract containing glucosamine of Example 1, this example provides a method for preparing an eggshell membrane extract containing glucosamine, comprising the following steps: Step 1, rehydrating the eggshell membrane; taking 1000 g of dried eggshell membrane, adding it into the inner tank body 2 through the raw material feed pipe 13 at a weight ratio of 1:30 between the eggshell membrane and purified water; Step 2, adding a reducing agent; adding a reducing agent to the inner tank body 2 through the raw material feed pipe 13 in a final concentration of 10% (w / v), starting the rotary drive device 3, and driving the raw material feed pipe 13, the filtrate feed pipe 15, and the stirring blade 17 to rotate to promote the dissolution of the reducing agent, wherein the reducing agent is ferrous sulfide or sodium sulfite; Step 3: Add a suitable pH adjustment liquid into the inner tank 2 through the raw material feed pipe 13 to adjust the pH value of the reaction system in the inner tank 2 to 7; Step 4, enzymolysis: alkaline protease is added to the inner tank 2 through the raw material feed pipe 13 at a final concentration of 1.5% (w / v), the enzymolysis temperature is 55° C., and the reaction time is 25 hours, which can provide a high degree of guarantee for the stability of product quality; Step 5, inactivate the enzyme; start the electric heater 25, the electric heater 25 heats the reaction system in the inner tank 2, and adopts a high temperature inactivation method to raise the temperature of the reaction system to 90 degrees Celsius and maintain it for 15 minutes; at this time, the reaction system has no obvious solid flaky shell film, and the reaction system is dark yellow; Step 6: Activated carbon insulation; 6.1, the reaction system is rapidly cooled to 48 degrees Celsius; specifically, the dehumidifier 26 delivers dry air to the main air supply pipe 27, and the main air supply pipe 27 passes the dry air into the booster air pipe 10 and the dry air supply pipe 28; on the one hand, the booster air pipe 10 passes the dry air into the inner tank body 2, and the heat dissipation valve 43 is opened. After the dry air exchanges heat with the reaction system in the inner tank body 2, it is discharged from the heat dissipation port 42 to cool the reaction system; on the other hand, the outer air supply pipe 30 passes the dry air into the outer interlayer 23, and then sprays it out from the outer spray head 21, and the inner air supply pipe 31 passes the dry air into the upper interlayer, and then sprays it out from the inner spray head 20; the annular cavity 12 is cooled, and the gas is finally discharged from the exhaust port 4; 6.2, adding activated carbon to the inner tank 2 through the raw material feed pipe 13 in a final concentration of 4% (w / v); 6.3, drive the stirring blade 17 to rotate by the rotary drive device 3, so that the activated carbon and the reaction system are fully mixed, and stir for 30 minutes under the heat preservation condition; the activated carbon can remove the odor and impurities generated during the reaction; Step seven, filtration and low-temperature spray drying; the air supply system ventilates the inner tank body 2 through the booster air pipe 10, so that the internal pressure of the inner tank body 2 increases, and the filtrate entering the filter cartridge 18 is pressed into the filtrate feeding pipe 15 and the atomizing nozzle 16, and the atomizing nozzle 16 enters the high-pressure filtrate and high-pressure air, and the filtrate feeding pipe 15 and the atomizing nozzle 16 are driven by the rotary drive device 3 to rotate in the annular cavity 12, so that the atomizing nozzle 16 sprays uniformly into the annular cavity 12; the filtrate feeding pipe 15 drives the stirring blade 17 and the filter cartridge 18 to rotate at the same time to prevent the activated carbon from clogging the filter holes of the filter cartridge 18; at the same time, the air supply system supplies air to the inner nozzle 20 and the outer nozzle 21, and the low-temperature dry air sprayed from the inner nozzle 20 and the outer nozzle 21 is fully in contact with the atomized filtrate sprayed from the atomizing nozzle 16, forming local turbulence, thereby improving the contact effect between the atomized filtrate and the drying gas, improving the drying efficiency, and making the water evaporate rapidly The gas discharged from the exhaust port 4 enters the cyclone dust collector 45 through the exhaust pipe 44, and the water vapor is discharged from the top of the cyclone dust collector 45, and the eggshell membrane extract particles fall into the storage box 48; during this period, the first air heater 32 and the first air heater 32 are powered on; the first air heater 32 heats the air transported by the drying air supply pipe 28, thereby controlling the temperature of the air sprayed from the outer nozzle 21 and the inner nozzle 20, and controlling the air inlet temperature of the drying air supply pipe 28 to be 55°C; the second air heater 36 heats the air transported by the boost air pipe 10, and controls the air inlet temperature of the boost air pipe 10 to be 45°C, so as to avoid the air temperature entering the inner tank body 2 being too low, resulting in the temperature of the filtrate in the inner tank body 2 being reduced, and ensuring that the temperature of the filtrate sprayed from the atomizing nozzle 16 is not too low, thereby improving the spray drying efficiency; Step 8, unloading: open the finished product unloading valve 6, so that the eggshell membrane extract containing glucosamine in the outer tank 1 is discharged from the finished product discharge port 5; open the filter residue unloading valve 8, so that the filter residue in the inner tank 2 is discharged from the filter residue discharge pipe 7. Low-temperature spray drying can effectively reduce the influence of high temperature of traditional spray drying on the active ingredients in eggshell membranes; the eggshell membrane hydrolyzate is light yellow in color and has high solubility. Example 3

[0035] The present application is substantially the same as Example 2, except that in step 2, a reducing agent is added to the inner tank 2 through the raw material feed pipe 13 at a final concentration of 5% (w / v); In step 3, the pH value of the reaction system in the inner tank 2 is adjusted to 6.5; In step 4, alkaline protease is added to the inner tank 2 through the raw material feed pipe 13 at a final concentration of 0.5% (w / v), the enzymatic hydrolysis temperature is 60° C., and the reaction time is 25 hours; In step 5, the temperature of the reaction system was raised to 90 degrees Celsius and maintained for 15 minutes; In step six, 6.1, the reaction system is rapidly cooled to 50 degrees Celsius; 6.2, activated carbon is added to the inner tank 2 through the raw material feed pipe 13 in a final concentration of 1% (w / v); In step seven, the air inlet temperature of the drying air supply pipe 28 is controlled to be 55°C, the air inlet temperature of the boost air pipe 10 is controlled to be 40°C, and the air outlet temperature of the exhaust port 4 is controlled to be 35°C. Example 4

[0036] The present application is substantially the same as Example 2, except that in step 2, a reducing agent is added to the inner tank 2 through the raw material feed pipe 13 in a final concentration of 7% (w / v); In step 3, the pH value of the reaction system in the inner tank 2 is adjusted to 6.8; In step 4, alkaline protease is added to the inner tank 2 through the raw material feed pipe 13 at a final concentration of 1.5% (w / v), the enzymatic hydrolysis temperature is 45° C., and the reaction time is 22 hours; In step 5, the temperature of the reaction system was raised to 80 degrees Celsius and maintained for 15 minutes; In step six, 6.1, the reaction system is rapidly cooled to 50 degrees Celsius; 6.2, activated carbon is added to the inner tank 2 through the raw material feed pipe 13 in a final concentration of 1% (w / v); In step seven, the air inlet temperature of the drying air supply pipe 28 is controlled to be 60°C, and the air inlet temperature of the boost air pipe 10 is controlled to be 40°C.

[0037] The yields of the eggshell membrane hydrolysates obtained in Examples 2 to 4 were measured, and it was found that the eggshell membrane hydrolysate obtained in Example 2 was the best. Compared with Examples 2 to 4, under the parameter conditions set in Example 1, the eggshell membrane hydrolysate was most suitable for extraction. The amino acid content and molecular weight distribution of the eggshell membrane hydrolysate obtained in Example 2 were detected as follows: Amino acid content: Asp, Thr, Ser, Glu, Gly, Ala, Val, Cys, Ile, Leu, Tyr, Phe, Lys, His, Arg, Pro, Met are 2.03, 7.27, 4.79, 4.21, 10.99, 4.99, 2.41, 5.93, 7.73, 2.99, 4.01, 1.81, 1.71, 3.11, 3.37, 6.31, 7.11, 2.95 respectively; unit: g / 100g; Molecular weight distribution: A=6.48%, B=7.62%, C=15.39%, D=38.5%, E=32.01%. A molecular weight is greater than 2000, B molecular weight is 1000 to 2000, C molecular weight is greater than 500 to 1000, D molecular weight is greater than 189 to 500, and E molecular weight is less than 189.

[0038] It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present invention, and these modifications and improvements all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

[0039] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0040] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0041] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

Claims

1. A device for preparing an eggshell membrane extract containing glucosamine, characterized in that: The invention comprises an outer tank body (1), an inner tank body (2), and a rotary drive device (3); the outer tank body (1) is provided with an exhaust port (4) at the top, the outer tank body (1) is provided with a finished product discharge port (5) at the bottom, a finished product discharge valve (6) is installed on the finished product discharge port (5), the inner tank body (2) is fixed inside the outer tank body (1), a filter residue discharge pipe (7) is provided at the bottom of the inner tank body (2), a filter residue discharge valve (8) is installed on the top of the filter residue discharge pipe (7), a pressurized air pipe (10) is connected to the filter residue discharge pipe (7) and located above the filter residue discharge valve (8), and the pressurized air pipe (10) is close to the filter residue discharge pipe (7). A one-way valve (11) is installed near the filter residue discharge pipe (7). The lower end of the filter residue discharge pipe (7) extends out of the outer tank body (1). An annular cavity (12) is formed between the inner tank body (2) and the outer tank body (1). A raw material feed pipe (13) is rotatably and sealably installed on the outer tank body (1). A feed valve (14) is installed on the upper part of the raw material feed pipe (13). The lower end of the raw material feed pipe (13) extends into the inner tank body (2) and is rotatably and sealably connected to the inner tank body (2). A filtrate feed pipe (15) is provided in the inner tank body (2). The filtrate feed pipe (15) is fixedly connected to the raw material feed pipe (13). The upper end of the filtrate feeding pipe (15) extends from the upper part of the inner tank body (2) to form a raw material feeding pipe (13) and is equipped with an atomizing nozzle (16). The rotary drive device (3) drives the raw material feeding pipe (13) to rotate so that the atomizing nozzle (16) rotates in the annular cavity (12). The lower end of the filtrate feeding pipe (15) is fixed with a stirring blade (17) and a filter cartridge (18) in sequence from top to bottom. The filter cartridge (18) is connected to the raw material feeding pipe (13). The inner tank body (2) is provided with an inner interlayer (19). A plurality of inner spray nozzles (20) are installed in the inner interlayer (19). The inner spray nozzles (20) deliver The outlet end is located in the annular cavity (12), a plurality of external nozzles (21) are installed on the outer wall of the outer tank body (1), the output end of the external nozzle (21) is located in the annular cavity (12), the booster air pipe (10), the internal nozzle (20) and the external nozzle (21) are all connected to the air supply system, the angle between the tangent of the axis of the internal nozzle (20) and the axis of the atomizing nozzle (16) is less than 90 degrees, and the angle between the tangent of the axis of the external nozzle (21) and the axis of the atomizing nozzle (16) is less than 90 degrees.

2. The device for preparing the eggshell membrane extract containing glucosamine according to claim 1, characterized in that: The outer jet heads (21) installed on the outer wall of the outer tank body (1) are arranged in multiple layers, and the outer jet heads (21) of each layer are distributed circumferentially on the outer wall of the outer tank body (1); the inner jet heads (20) installed in the inner interlayer (19) are arranged in multiple layers, and the inner jet heads (20) of each layer are distributed circumferentially in the inner interlayer (19).

3. The device for preparing the eggshell membrane extract containing glucosamine according to claim 2, characterized in that: An air intake sleeve (22) is fixed on the outer wall of the outer tank body (1), an outer interlayer (23) is formed between the air intake sleeve (22) and the outer tank body (1), and the outer jet heads (21) are all located in the outer interlayer (23).

4. The device for preparing the eggshell membrane extract containing glucosamine according to claim 3, characterized in that: A partition (24) is fixed in the inner interlayer (19), and the partition (24) divides the inner interlayer (19) into an upper interlayer and a lower interlayer. The inner jet head (20) is located in the upper interlayer, and an electric heater (25) is installed in the lower interlayer.

5. The device for preparing the eggshell membrane extract containing glucosamine according to claim 4, characterized in that: The gas supply system comprises a dehumidifier (26), a main gas supply pipe (27), a dry gas supply pipe (28), a gas booster (29), an external gas supply pipe (30) and an internal gas supply pipe (31). The main gas supply pipe (27) is installed at the output end of the dehumidifier (26). The main gas supply pipe (27) is installed with a gas booster (29). The output end of the main gas supply pipe (27) is connected to the booster pipe (10) and the dry gas supply pipe (28). The dry gas supply pipe (28) is connected to the external gas supply pipe (30). 0) and an inner air supply pipe (31), the outer air supply pipe (30) is in communication with the outer interlayer (23), the inner air supply pipe (31) is in communication with the upper interlayer, a first air heater (32), a first regulating valve (33), a first temperature sensor (34), and a first air pressure sensor (35) are installed on the drying air supply pipe (28); and a second air heater (36), a second regulating valve (37), a second temperature sensor (38), and a second air pressure sensor (39) are installed on the boost air pipe (10).

6. The device for preparing the eggshell membrane extract containing glucosamine according to claim 1, characterized in that: A third temperature sensor (40) and a liquid level meter (41) are installed in the inner tank body (2). A heat dissipation port (42) is provided at the top of the inner tank body (2). A heat dissipation valve (43) is installed on the heat dissipation port (42).

7. The device for preparing an eggshell membrane extract containing glucosamine according to claim 1, characterized in that: The exhaust port (4) is connected to a cyclone dust collector (45) via an exhaust pipe (44), an exhaust valve (46) and a fourth temperature sensor (47) are installed on the exhaust pipe (44), a material storage box (48) is connected to the bottom of the cyclone dust collector (45), a material discharge port (49) is provided at the bottom of the material storage box (48), and a material discharge valve (50) is installed on the material discharge port (49).

8. The device for preparing an eggshell membrane extract containing glucosamine according to claim 1, characterized in that: A protective shell (51) is installed at the top of the outer tank body (1), the rotary drive device (3) is fixedly installed on the protective shell (51), the output end of the rotary drive device (3) extends into the protective shell (51) and is installed with a driving pulley (52), the raw material feed pipe (13) is rotatably connected to the protective shell (51) via a bearing, the raw material feed pipe (13) passes through the protective shell (51), a driven pulley (53) is installed on the portion of the raw material feed pipe (13) located inside the protective shell (51), and the driving pulley (52) and the driven pulley (53) are connected to each other via a transmission belt (54).

9. The device for preparing an eggshell membrane extract containing glucosamine according to claim 1, characterized in that: The filter cartridge (18) comprises a conical cylinder portion (55) and a cylindrical portion (56); the lower end of the conical cylinder portion (55) is adjacent to the upper end of the cylindrical portion (56); the diameter of the upper end of the conical cylinder portion (55) is greater than the diameter of the cylindrical portion (56); and the cylindrical portion (56) is located inside the filter residue discharge pipe (7) and above the filter residue discharge valve (8).

10. A method for preparing an eggshell membrane extract containing glucosamine using the device for preparing an eggshell membrane extract containing glucosamine according to any one of claims 1 to 9, characterized in that: The steps include: Step 1: rehydrating the eggshell membrane; taking dried eggshell membrane and adding it into the inner tank (2) through the raw material feed pipe (13) at a weight ratio of 1:20 to 50 between eggshell membrane and purified water; Step 2: Adding a reducing agent; adding the reducing agent to the inner tank body (2) through the raw material feed pipe (13) in a final concentration of 5 to 20% (w / v), and driving the stirring blade (17) to rotate through the rotary drive device (3) to promote the dissolution of the reducing agent, wherein the reducing agent is ferrous sulfide or sodium sulfite; Step 3: Adjust the pH value of the reaction system in the inner tank (2) to 6.5-7.5; Step 4: enzymolysis: adding alkaline protease to the inner tank (2) through the raw material feed pipe (13) at a final concentration of 0.5-3% (w / v), the enzymolysis temperature is 45-70° C., and the reaction time is 12-36 hours, which can provide a high degree of guarantee for the stability of product quality; Step 5: Inactivate the enzyme by heating the reaction system to 80-100 degrees Celsius and maintaining the temperature for 10-20 minutes. Step 6: Activated carbon insulation; 6.1 Rapidly cool the reaction system to 40-60 degrees Celsius; 6.2 Adding activated carbon to the inner tank (2) through the raw material feed pipe (13) in a final concentration of 0.5-5% (w / v); 6.3 The stirring blade (17) is driven to rotate by the rotary drive device (3) so that the activated carbon and the reaction system are fully mixed; Step seven, filtering and low-temperature spray drying; the air supply system ventilates the inner tank body (2) through the booster air pipe (10), so that the internal pressure of the inner tank body (2) increases, and the filtrate entering the filter cartridge (18) is pressed into the filtrate feeding pipe (15) and the atomizing nozzle (16), and the atomizing nozzle (16) is driven by the rotary drive device (3) to rotate in the annular cavity (12), and the atomizing nozzle (16) sprays into the annular cavity (12); the air supply system supplies air to the inner spray nozzle (20) and the outer spray nozzle (21), and the low-temperature dry air sprayed from the inner spray nozzle (20) and the outer spray nozzle (21) contacts the atomized filtrate sprayed from the atomizing nozzle (16), so that the water evaporates rapidly and is discharged from the exhaust port (4), thereby obtaining an eggshell membrane extract containing glucosamine components; Step eight, unloading; opening the finished product unloading valve (6) to discharge the eggshell membrane extract containing glucosamine in the outer tank body (1) from the finished product discharge port (5); opening the filter residue unloading valve (8) to discharge the filter residue in the inner tank body (2) from the filter residue discharge pipe (7).

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