An inoculation device for efficiently inoculating microbial bacterial liquid in a large-sized container

Abstract

The present invention discloses an inoculation device for efficiently inoculating microbial liquid in a large-sized container. The present invention relates to the technical field of efficiently inoculating microbial liquid in a large-sized container. It includes a fixing and rotating assembly arranged on a workbench for fixing and driving the large-sized container to rotate around its own axis, and a servo motor fixed on the bottom surface of the workbench. The output shaft of the servo motor penetrates the workbench upward, and a main shaft is connected to the extending end. A strip-shaped plate is fixedly arranged at the top of the main shaft. An automatic cover removal assembly for removing the plastic cover of the large-sized container is arranged at the left end of the strip-shaped plate. The automatic cover removal assembly is located directly above the fixing and rotating assembly. An automatic dispersion assembly for dispersing the microbial liquid in a flower shape in the container body of the large-sized container is arranged at the right end of the strip-shaped plate. The beneficial effects of the present invention are: the plastic cover is tightly closed, and the inoculation efficiency of the microbial liquid is greatly improved.

Description

Technical Field

[0001] The present invention relates to the technical field of efficiently inoculating microbial liquid in a large-sized container, and particularly to an inoculation device for efficiently inoculating microbial liquid in a large-sized container. Background Art

[0002] Microbial inoculation is to disperse a certain amount of microbial liquid in the culture medium in a culture dish. After cultivation, more independent microbial colonies are obtained. Since the microbial colonies are visible to the naked eye, it is convenient for staff to extract valuable components from the microbial colonies. Since the diameter of the culture dish is generally less than 15 cm and it is impossible to inoculate a large volume of microbial liquid, therefore, the staff uses a large-sized container as shown in Figures 1 to 4 to inoculate the microbial liquid. The large-sized container includes a container body 1 and a plastic cover 2 covering the top port of the container body 1. The bottom of the container body 1 is closed, and the diameter of the container body 1 is 25 cm.

[0003] The method for the staff to inoculate the microbial liquid in the large-sized container:

[0004] S1. The staff takes out a large-sized container and supports the container body 1 of the large-sized container on a table; then the staff detaches the plastic cover 2 from the container body 1;

[0005] S2. The staff pours a certain amount of culture medium into the container body 1;

[0006] S3. The staff takes out an inoculator, uses the inoculator to extract a certain amount of microbial liquid from a storage tank; then contacts the liquid outlet port of the inoculator with the liquid surface of the culture medium; the staff squeezes the airbag of the inoculator, and the microbial liquid in the inoculator flows out from the liquid outlet port of the inoculator. Then the staff moves the inoculator in a flower shape. When the inoculator is moved back to the initial position, the first batch of microbial liquid can be dispersed in the culture medium in the container body 1 in a flower shape;

[0007] S4. The staff repeats the operation of step S3 multiple times to disperse multiple batches of microbial liquid in the culture medium in the container body 1 in a flower shape;

[0008] S5. The staff re-covers the plastic cover 2 on the top port of the container body 1 to seal the culture medium and the microbial liquid, and finally realizes inoculating the microbial liquid in the large-sized container;

[0009] S6. The staff repeats the operations of steps S1 - S5 multiple times to inoculate the microbial liquid in multiple large-sized containers.

[0010] However, although this method can inoculate the microbial liquid in a large-sized container, in actual operation, the following technical defects are still reflected:

[0011] I. Among them, in step S1, when the staff uses their left and right hands to respectively pull up the bottom surface of the side of the plastic cover 2, the pulling directions are as Figure 2 shown by the solid arrows. Since the top of the plastic cover 2 is still tightly attached to the top surface of the container body 1, the top of the plastic cover 2 blocks the upward movement of the side of the plastic cover 2, resulting in the side of the plastic cover 2 deforming outward. As a result, in step S5, after the staff re-covers the plastic cover 2 on the top port of the container body 1, the plastic cover 2 cannot seal the top port of the container body 1, that is, there is a gap between the side of the plastic cover 2 and the cylindrical surface of the container body 1. Therefore, there is a technical defect that the plastic cover 2 is not tightly covered.

[0012] In addition, not only does it require manual removal of the plastic cover 2 from the container body 1, but it also requires manual re-covering of the plastic cover 2 on the container body 1; and the entire operation is completed manually, thus prolonging the inoculation time of the microbial liquid and reducing the inoculation efficiency of the microbial liquid.

[0013] II. In steps S3 - S4, the staff needs to first use an inoculator to extract a certain amount of microbial liquid from the storage tank, and then manually draw the inoculator in a flower shape to disperse the microbial liquid in a flower shape in the culture solution of the container body 1; and the entire operation is completed manually, and there are many operation procedures, which undoubtedly takes a long time to disperse multiple batches of microbial liquid in a flower shape in the culture solution of the container body 1, thereby further reducing the inoculation efficiency of the microbial liquid.

[0014] Therefore, there is an urgent need for an inoculation device that can tightly cover the plastic cover and greatly improve the inoculation efficiency of the microbial liquid. Summary of the Invention

[0015] The purpose of the present invention is to overcome the shortcomings of the prior art and provide an inoculation device for efficiently inoculating microbial liquid in a large-sized container that can tightly cover the plastic cover and greatly improve the inoculation efficiency of the microbial liquid.

[0016] The object of the present invention is achieved by the following technical solutions: An inoculation device for efficiently inoculating microbial liquid in a large-sized container, which includes a fixing and rotating assembly arranged on a workbench for fixing and driving the large-sized container to rotate around its own axis, a servo motor fixed on the bottom surface of the workbench, the output shaft of the servo motor penetrates the workbench upward, and a main shaft is connected to the extending end. A strip-shaped plate is fixedly arranged at the top of the main shaft. An automatic cover-removing assembly for removing the plastic cover of the large-sized container is arranged at the left end of the strip-shaped plate. The automatic cover-removing assembly is located directly above the fixing and rotating assembly. An automatic dispersing assembly for dispersing the microbial liquid in a flower shape in the container body of the large-sized container is arranged at the right end of the strip-shaped plate;

[0017] The automatic dispersing assembly includes a lifting cylinder fixed at the right end of the strip-shaped plate. The piston rod of the lifting cylinder penetrates the strip-shaped plate downward, and a lifting plate is fixedly arranged at the extending end. An L-shaped plate is fixedly arranged on the bottom surface of the left end of the lifting plate. An annular ring is fixedly arranged at the end of the L-shaped plate. A plurality of arc-shaped grooves spaced apart from each other are formed on the inner wall of the central hole of the annular ring along its circumference. A driving motor is fixedly arranged on the bottom surface of the right end of the lifting plate. A connecting plate that penetrates the central hole of the annular ring downward is fixedly arranged on the output shaft of the driving motor;

[0018] A movable strip extending to the right slidably penetrates through the lower end of the connecting plate. A fixing plate, an inoculation tube, and a rod are fixedly arranged on the top surface of the movable strip in sequence from left to right. A spring is fixedly arranged between the fixing plate and the connecting plate; the top port of the inoculation tube penetrates the central hole of the annular ring upward and extends above the annular ring. A nylon wheel is rotatably installed at the top of the rod. Under the elastic force of the spring, the nylon wheel abuts against the bottom of the rightmost arc-shaped groove in the annular ring;

[0019] A storage tank storing microbial liquid is fixedly arranged on the left end surface of the connecting plate. A peristaltic pump is fixedly arranged on the bottom surface of the connecting plate. A liquid suction pipe is connected to the liquid suction port of the peristaltic pump. The liquid suction pipe penetrates the connecting plate and is connected to the bottom port of the storage tank. A liquid discharge pipe is connected to the liquid discharge port of the peristaltic pump. The liquid discharge pipe is connected to the top port of the inoculation tube.

[0020] The axis of the driving motor is coaxial with the axis of the annular ring.

[0021] The arc-shaped grooves are evenly distributed on the inner wall of the annular ring.

[0022] A support plate is fixedly arranged on the left end surface of the connecting plate. The storage tank is fixedly arranged on the support plate.

[0023] The fixed and rotating assembly includes a stepper motor fixed on the workbench surface, an arch-shaped frame A fixed on the output shaft of the stepper motor. A turntable is fixed on the top of the arch-shaped frame A. A positioning hole is formed on the top surface of the turntable. The diameter of the positioning hole is equal to the outer diameter of the container body of the large-sized container. A stepped hole penetrating the bottom surface of the turntable is formed at the bottom of the positioning hole. A suction cup A is fixed in the large hole of the stepped hole. The top surface of the suction cup A is flush with the bottom of the positioning hole. A plurality of vacuum holes A communicating with its inner cavity are formed on the top surface of the suction cup A. A vacuum pump A is fixed in the arch-shaped frame A. The working port of the vacuum pump A is communicated with the inner cavity of the suction cup A through a pipeline.

[0024] The automatic cover removal assembly includes a cover removal cylinder fixed at the left end of the strip-shaped plate. The piston rod of the cover removal cylinder penetrates downward through the strip-shaped plate, and an arch-shaped frame B is fixed on the extending end. A suction cup B is fixed on the bottom surface of the arch-shaped frame B. A plurality of vacuum holes B communicating with its inner cavity are formed on the bottom surface of the suction cup B. A vacuum pump B is fixed in the arch-shaped frame B. The working port of the vacuum pump B is communicated with the inner cavity of the suction cup B.

[0025] Horizontal cylinders are fixed on the top surface of the suction cup B and on both its left and right sides. The piston rods of the two horizontal cylinders extend outside the suction cup B, and vertical plates are fixed on the extending ends. Longitudinally arranged lifting plates are fixed on the inner end faces of the two vertical plates. Semi-circular holes are formed on the inner end faces of the two lifting plates. The diameter of the semi-circular holes is equal to the outer diameter of the container body of the large-sized container.

[0026] A plurality of legs supporting on the ground are fixed on the bottom surface of the workbench.

[0027] The inoculation device further includes a controller, which is electrically connected to the servo motor, drive motor, stepper motor, lifting cylinder, cover removal cylinder, horizontal cylinder, peristaltic pump, vacuum pump A, and vacuum pump B through signal lines.

[0028] The present invention has the following advantages: The plastic cover is tightly closed, and the inoculation efficiency of the microbial liquid is greatly improved. Description of the Drawings

[0029] Figure 1 It is a structural schematic diagram of a large-sized container;

[0030] Figure 2 For Figure 1 the main sectional schematic diagram;

[0031] Figure 3 It is a structural schematic diagram of the container body of the large-sized container;

[0032] Figure 4 It is a structural schematic diagram of the plastic cover of the large-sized container;

[0033] Figure 5Schematic diagram of dispersing the microbial liquid of the first batch in a flower shape in the culture solution of the container body;

[0034] Figure 6 Schematic diagram of dispersing the microbial liquid of multiple batches in a flower shape in the culture solution of the container body;

[0035] Figure 7 Schematic structural diagram of the present invention;

[0036] Figure 8 For Figure 7 Main sectional view of;

[0037] Figure 9 Schematic structural diagram of the fixing and rotating assembly;

[0038] Figure 10 For Figure 9 Bottom view of;

[0039] Figure 11 For Figure 10 Main sectional view of;

[0040] Figure 12 Schematic structural diagram of the automatic cover removal assembly;

[0041] Figure 13 For Figure 11 Main sectional view of;

[0042] Figure 14 Schematic structural diagram of the automatic dispersion assembly;

[0043] Figure 15 For Figure 14 Bottom view of;

[0044] Figure 16 For Figure 14 Main sectional view of;

[0045] Figure 17 Schematic diagram of the ring structure;

[0046] Figure 18 Schematic diagram of the connection of the connecting plate, movable strip, nylon wheel, spring and inoculation tube;

[0047] Figure 19 Schematic diagram of inserting the container body of a large-sized container into the positioning hole of the fixing and rotating assembly;

[0048] Figure 20 Schematic diagram of the bottom surface of the suction cup B contacting the top of the plastic cover;

[0049] Figure 21 For Figure 20 Partial enlarged view of the M part of;

[0050] Figure 22 Schematic diagram of the semi-circular holes of two lifting plates respectively stuck on the outer cylindrical surface of the container body;

[0051] Figure 23 For Figure 22 Partial enlarged view of the N part of ;

[0052] Figure 24 Schematic diagram of automatically detaching the plastic lid from the container body;

[0053] Figure 25 Schematic diagram of the inoculation tube of the automatic dispersion assembly moving to directly above the container body;

[0054] Figure 26 Schematic diagram of the bottom port of the inoculation tube contacting the liquid surface of the culture solution in the container body;

[0055] Figure 27 For Figure 26 Partial enlarged view of the P part of ;

[0056] Figure 28 Schematic diagram of the turntable driving the container body to rotate synchronously;

[0057] Figure 29 Schematic diagram of the plastic lid being re-covered on the container body;

[0058] Figure 30 Schematic diagram of the staff taking away the large-sized container inoculated with microbial liquid;

[0059] In the figure:

[0060] 1 - Container body, 2 - Plastic lid;

[0061] 3 - Workbench, 4 - Fixing and rotating assembly, 5 - Servo motor, 6 - Main shaft, 7 - Strip plate, 8 - Automatic lid removal assembly, 9 - Automatic dispersion assembly;

[0062] 10 - Lifting cylinder, 11 - Lifting plate, 12 - L-shaped plate, 13 - Ring, 14 - Arc groove, 15 - Driving motor, 16 - Connecting plate, 17 - Movable bar, 18 - Fixed plate, 19 - Inoculation tube, 20 - Rod, 21 - Spring, 22 - Nylon wheel, 23 - Storage tank, 24 - Peristaltic pump, 25 - Liquid suction pipe, 26 - Liquid discharge pipe;

[0063] 27 - Stepper motor, 28 - Turntable, 29 - Positioning hole, 30 - Suction cup A, 31 - Vacuum hole A, 32 - Vacuum pump A;

[0064] 33 - Lid removal cylinder, 34 - Suction cup B, 35 - Vacuum hole B, 36 - Vacuum pump B, 37 - Horizontal cylinder, 38 - Lifting plate, 39 - Semi-circular hole. Detailed implementation mode

[0065] The following further describes the present invention in conjunction with the accompanying drawings. The protection scope of the present invention is not limited to the following:

[0066] As Figures 7 to 18 shown, an inoculation device for efficiently inoculating microbial liquid in a large-sized container includes a fixing and rotating assembly 4 disposed on a workbench 3 for fixing and driving the large-sized container to rotate around its own axis, and a servo motor 5 fixedly disposed on the bottom surface of the workbench 3. The output shaft of the servo motor 5 penetrates the workbench 3 upward, and a main shaft 6 is connected to the extending end. A strip plate 7 is fixedly disposed at the top of the main shaft 6. An automatic cover removing assembly 8 for removing the plastic cover 2 of the large-sized container is disposed at the left end of the strip plate 7. The automatic cover removing assembly 8 is located directly above the fixing and rotating assembly 4. An automatic dispersion assembly 9 for dispersing the microbial liquid in a flower shape in the container body 1 of the large-sized container is disposed at the right end of the strip plate 7. A plurality of legs supporting on the ground are fixedly disposed on the bottom surface of the workbench 3.

[0067] The automatic dispersion assembly 9 includes a lifting cylinder 10 fixedly disposed at the right end of the strip plate 7. The piston rod of the lifting cylinder 10 penetrates the strip plate 7 downward, and a lifting plate 11 is fixedly disposed at the extending end. An L-shaped plate 12 is fixedly disposed on the bottom surface of the left end of the lifting plate 11. An annular ring 13 is fixedly disposed at the end of the L-shaped plate 12. A plurality of arc-shaped grooves 14 spaced apart are formed on the inner wall of the central hole of the annular ring 13 along its circumference. The arc-shaped grooves 14 are evenly distributed on the inner wall of the annular ring 13. A driving motor 15 is fixedly disposed on the bottom surface of the right end of the lifting plate 11. A connecting plate 16 penetrating downward through the central hole of the annular ring 13 is fixedly disposed on the output shaft of the driving motor 15. The axis of the driving motor 15 is coaxial with the axis of the annular ring 13.

[0068] A movable strip 17 extending rightward is slidably penetrated through the lower end of the connecting plate 16. A fixing plate 18, an inoculation tube 19, and a rod member 20 are fixedly disposed on the top surface of the movable strip 17 in sequence from left to right. A spring 21 is fixedly disposed between the fixing plate 18 and the connecting plate 16. The top port of the inoculation tube 19 penetrates the central hole of the annular ring 13 upward and extends above the annular ring 13. A nylon wheel 22 is rotatably mounted at the top of the rod member 20. Under the elastic force of the spring 21, the nylon wheel 22 abuts against the bottom of the rightmost arc-shaped groove 14 in the annular ring 13. A storage tank 23 storing microbial liquid is fixedly disposed on the left end surface of the connecting plate 16. A peristaltic pump 24 is fixedly disposed on the bottom surface of the connecting plate 16. A liquid suction pipe 25 is connected to the liquid suction port of the peristaltic pump 24. The liquid suction pipe 25 penetrates the connecting plate 16 and is connected to the bottom port of the storage tank 23. A liquid discharge pipe 26 is connected to the liquid discharge port of the peristaltic pump 24. The liquid discharge pipe 26 is connected to the top port of the inoculation tube 19. A support plate is fixedly disposed on the left end surface of the connecting plate 16. The storage tank 23 is fixedly disposed on the support plate.

[0069] The fixed and rotating assembly 4 includes a stepper motor 27 fixed on the tabletop of the workbench 3, an arched frame A fixed on the output shaft of the stepper motor 27. A turntable 28 is fixed on the top of the arched frame A. A positioning hole 29 is provided on the top surface of the turntable 28. The diameter of the positioning hole 29 is equal to the outer diameter of the container body 1 of the large-size container. A stepped hole penetrating the bottom surface of the turntable 28 is provided at the bottom of the positioning hole 29. A suction cup A30 is fixed in the large hole of the stepped hole. The top surface of the suction cup A30 is flush with the bottom of the positioning hole 29. A plurality of vacuum holes A31 communicating with its inner cavity are provided on the top surface of the suction cup A30; A vacuum pump A32 is fixed in the arched frame A. The working port of the vacuum pump A32 is communicated with the inner cavity of the suction cup A30 through a pipeline.

[0070] The automatic lid-removing assembly 8 includes a lid-removing cylinder 33 fixed at the left end of the strip plate 7. The piston rod of the lid-removing cylinder 33 penetrates the strip plate 7 downward, and an arched frame B is fixed on the extending end. A suction cup B34 is fixed on the bottom surface of the arched frame B. A plurality of vacuum holes B35 communicating with its inner cavity are provided on the bottom surface of the suction cup B34. A vacuum pump B36 is fixed in the arched frame B. The working port of the vacuum pump B36 is communicated with the inner cavity of the suction cup B34; On the top surface of the suction cup B34 and on its left and right sides, horizontal cylinders 37 are fixed. The piston rods of the two horizontal cylinders 37 both extend outside the suction cup B34, and vertical plates are fixed on the extending ends. Lifting plates 38 arranged longitudinally are fixed on the inner end faces of the two vertical plates. Semi-circular holes 39 are provided on the inner end faces of the two lifting plates 38. The diameter of the semi-circular hole 39 is equal to the outer diameter of the container body 1 of the large-size container.

[0071] This inoculation device further includes a controller, which is electrically connected to the servo motor 5, the driving motor 15, the stepper motor 27, the lifting cylinder 10, the lid-removing cylinder 33, the horizontal cylinder 37, the peristaltic pump 24, the vacuum pump A32, and the vacuum pump B36 through signal lines. The staff can control the start or stop of the servo motor 5, the driving motor 15, the stepper motor 27, the peristaltic pump 24, the vacuum pump A32, and the vacuum pump B36 through the controller. At the same time, it can also control the extension or retraction of the piston rods of the lifting cylinder 10, the lid-removing cylinder 33, and the horizontal cylinder 37, thus facilitating the operation of the staff and having the characteristics of high automation.

[0072] The working steps of the present invention are as follows:

[0073] S1. The staff takes out a large-size container as shown in Figures 1 to 2 and inserts the container body 1 of the large-size container into the positioning hole 29 of the turntable 28 of the fixed and rotating assembly 4 from top to bottom, as shown in Figure 19As shown, at this time, the large-sized container enters the lid-removing station of the automatic lid-removing assembly 8. The bottom surface of the container body 1 of the large-sized container completely covers all the vacuum holes A31 of the suction cup A30. At the same time, the plastic lid 2 of the large-sized container is directly above the positioning hole 29, and the plastic lid 2 is just directly above the suction cup B34 of the automatic lid-removing assembly 8;

[0074] S2. Remove the plastic lid 2 from the container body 1. The specific operation steps are as follows:

[0075] S21. Control the vacuum pump A32 of the fixing and rotating assembly 4 to start. The vacuum pump A32 evacuates the inner cavity and each vacuum hole A31 of the suction cup A30. Under negative pressure, the container body 1 of the large-sized container is adsorbed and fixed on the suction cup A30;

[0076] S22. Control the piston rod of the lid-removing cylinder 33 of the automatic lid-removing assembly 8 to extend downward. The piston rod drives the arched frame B to move downward. The arched frame B drives the vacuum pump B36, the suction cup B34, the two horizontal cylinders 37 and the lifting plate 38 to move downward synchronously; when the piston rod of the lid-removing cylinder 33 is fully extended, the bottom surface of the suction cup B34 just contacts the top of the plastic lid 2, as Figures 20 to 21 shown. At the same time, the semi-circular holes 39 of the two lifting plates 38 are respectively on the left and right sides of the container body 1 of the large-sized container;

[0077] S23. Control the piston rods of the two horizontal cylinders 37 to retract. The piston rods drive the vertical plates to move toward the container body 1. The vertical plates drive the semi-circular holes 39 of the lifting plates 38 to move synchronously toward the container body 1. When the piston rods of the two horizontal cylinders 37 are fully retracted, the semi-circular holes 39 of the two lifting plates 38 are respectively stuck on the outer cylindrical surface of the container body 1, as Figures 22 to 23 shown. At the same time, the top surfaces of the two lifting plates 38 contact the bottom surface of the side part of the plastic lid 2;

[0078] S24. Control the vacuum pump B36 to start. The vacuum pump B36 evacuates the inner cavity and each vacuum hole B35 of the suction cup B34. Under negative pressure, the plastic lid 2 of the large-sized container is adsorbed and fixed on the suction cup B34;

[0079] S25. Control the piston rod of the lid-removing cylinder 33 of the automatic lid-removing assembly 8 to retract upward. The piston rod drives the arched frame B to move upward. The arched frame B drives the vacuum pump B36, the suction cup B34, the two horizontal cylinders 37 and the lifting plate 38 to move upward synchronously. Among them, the suction cup B34 drives the top of the plastic lid 2 to move upward. At the same time, the two lifting plates 38 drive the side part of the plastic lid 2 to move upward, thereby gradually removing the plastic lid 2 from the container body 1; when the piston rod of the lid-removing cylinder 33 is fully retracted, the plastic lid 2 can be automatically removed from the container body 1, as Figure 24 shown;

[0080] Among them, as can be seen in step S25, while the suction cup B34 drives the top of the plastic cover 2 to move upward, the two lifting plates 38 also lift the side of the plastic cover 2 upward, so that the top and side of the plastic cover 2 move upward synchronously. Therefore, compared with the manual lid removal method, this inoculation device effectively avoids the deformation of the side of the plastic cover 2 caused by the top of the plastic cover 2 blocking the upward movement of the side of the plastic cover 2 when the staff manually pulls up the bottom surface of the side of the plastic cover 2. Thus, it ensures that in S82, after the plastic cover 2 is re-covered on the top port of the container body 1, the plastic cover 2 can tightly seal the top port of the container body 1, featuring tight lid closure.

[0081] In addition, through the linkage cooperation of the fixing and rotating assembly 4 and the automatic lid removal assembly 8, this inoculation device can automatically remove the plastic cover 2 from the container body 1 or automatically cover the plastic cover 2 on the container body 1, without the need for manual removal of the plastic cover 2 from the container body 1 or manual re-covering of the plastic cover 2 on the container body 1. This shortens the inoculation time of the microbial liquid, thus greatly improving the inoculation efficiency of the microbial liquid.

[0082] S3. After removing the plastic cover 2 from the container body 1, the staff adds a certain amount of culture medium into the container body 1;

[0083] S4. Control the servo motor 5 to start. The servo motor 5 drives the main shaft 6 to rotate, the main shaft 6 drives the strip plate 7 to rotate, the strip plate 7 drives the automatic lid removal assembly 8 and the automatic dispersion assembly 9 to rotate synchronously, and the automatic lid removal assembly 8 drives the removed plastic cover 2 to rotate synchronously. When the strip plate 7 rotates 180°, the controller controls the servo motor 5 to turn off. At this time, the inoculation tube 19 of the automatic dispersion assembly 9 just moves to directly above the container body 1, as Figure 25 shown;

[0084] S5. Disperse the first batch of microbial liquid in a flower shape in the culture medium of the container body 1. The specific operation steps are as follows:

[0085] S51. Control the piston rod of the lifting cylinder 10 of the automatic dispersion assembly 9 to extend downward. The piston rod drives the lifting plate 11 to move downward, and the lifting plate 11 drives all the components connected below it to move downward synchronously. When the piston rod of the lifting cylinder 10 is fully extended, the bottom port of the inoculation tube 19 just contacts the liquid surface of the culture medium in the container body 1, as Figures 26 to 27 shown;

[0086] S52. Control the peristaltic pump 24 of the automatic dispersion component 9 to start. The peristaltic pump 24 pumps out the microbial liquid in the storage tank 23. Under the pump pressure, the microbial liquid sequentially passes through the liquid suction pipe 25, the peristaltic pump 24, the liquid discharge pipe 26, the top port of the inoculation pipe 19, the inoculation pipe 19, and finally discharges from the bottom port of the inoculation pipe 19. The discharged microbial liquid initially falls on the liquid surface of the culture solution in the container body 1;

[0087] S53. Control the drive motor 15 of the automatic dispersion component 9 to start. The drive motor 15 drives the connecting plate 16 to rotate around the axis of the drive motor 15. The connecting plate 16 drives the peristaltic pump 24, the storage tank 23, the movable bar 17, the fixing plate 18, the spring 21, and the nylon wheel 22 on it to rotate synchronously. Among them, the nylon wheel 22 rotates relative to the axis of the stationary annular ring 13, and the rotation direction is as Figure 27 shown by the solid arrow in the figure. During the rotation, the nylon wheel 22 moves in a wavy shape along each arc groove 14. The nylon wheel 22 moving in a wavy shape drives the rod 20 to make a reciprocating radial movement relative to the stationary annular ring 13. The rod 20 drives the movable bar 17 to make a reciprocating radial movement. The movable bar 17 drives the inoculation pipe 19 to make a reciprocating radial movement. When the nylon wheel 22 rotates one circle, the controller controls the drive motor 15 and the peristaltic pump 24 to turn off. Since the inoculation pipe 19 not only rotates relative to the axis of the stationary annular ring 13 but also makes a reciprocating radial movement relative to the stationary annular ring 13, the inoculation pipe 19 makes a flower-shaped movement above the liquid surface of the culture solution, and thus the microbial liquid flowing out from the bottom port of the inoculation pipe 19 is dispersed in a flower shape in the culture solution of the container body 1, and finally the microbial liquid of the first batch is dispersed in a flower shape in the culture solution of the container body 1, as Figure 5 shown;

[0088] S6. The staff controls the stepper motor 27 of the fixed and rotating component 4 to start. The stepper motor 27 drives the arched frame A, the turntable 28, and the suction cup A30 to rotate synchronously. The turntable 28 drives the container body 1 to rotate synchronously. The rotation direction of the container body 1 is as Figure 28 shown by the hollow arrow in the figure. When the container body 1 rotates to the set angle, the controller controls the stepper motor 27 to turn off. Then the staff repeats the operations of steps S52 - S53, and the microbial liquid of the second time can be dispersed in a flower shape in the culture solution of the container body 1;

[0089] S7. The staff repeats the operation of step S6 many times like this, and the microbial liquid of multiple batches can be dispersed in a flower shape in the culture solution of the container body 1, as Figure 6 shown;

[0090] S8. Re-cover the plastic cover 2 in the automatic cover removal component 8 on the top port of the container body 1. The specific operation steps are as follows:

[0091] S81. Control the servo motor 5 to start. The servo motor 5 drives the main shaft 6 to rotate. The main shaft 6 drives the strip plate 7 to rotate. The strip plate 7 drives the automatic cover removal assembly 8 and the automatic dispersion assembly 9 to rotate synchronously. The automatic cover removal assembly 8 drives the removed plastic cover 2 to rotate synchronously. When the strip plate 7 rotates 180°, the controller controls the servo motor 5 to turn off. At this time, the plastic cover 2 in the automatic cover removal assembly 8 just moves to directly above the container body 1;

[0092] S82. Control the piston rod of the cover removal cylinder 33 of the automatic cover removal assembly 8 to extend downward. When the piston rod of the cover removal cylinder 33 is fully extended, the plastic cover 2 is re-covered on the container body 1, as Figure 29 shown, thereby sealing the top port of the container body 1, and finally realizing the inoculation of the microbial liquid in the large-size container;

[0093] S9. Removal of the large-size container inoculated with the microbial liquid. The specific operation steps are as follows:

[0094] S91. Control the piston rods of the two horizontal cylinders 37 of the automatic cover removal assembly 8 to extend outward. The piston rods drive the vertical plate to move outward. The vertical plate drives the lifting plate 38 to move outward. The semi-circular hole 39 of the lifting plate 38 is separated from the container body 1; then control the vacuum pump B36 to turn off. At this time, the suction cup B34 no longer adsorbs the top of the plastic cover 2; then control the piston rod of the cover removal cylinder 33 to retract upward. The piston rod drives the suction cup B34 to reset;

[0095] S92. Control the vacuum pump A32 to turn off. At this time, the suction cup A30 no longer adsorbs the container body 1; then the staff takes away the large-size container inoculated with the microbial liquid. The taking-away direction is as Figure 30 shown by the arrow in;

[0096] S10. If a person repeats the operations of steps S1 - S9 multiple times, the microbial liquid can be inoculated in multiple large-size containers.

[0097] Among them, in step S5, the staff only need to use the linkage of the lifting cylinder 10 of the automatic dispersion component 9 and the driving motor 15, so that the inoculation tube 19 can not only rotate relative to the axis of the stationary annular ring 13, but also make reciprocating radial movement relative to the stationary annular ring 13, so that the inoculation tube 19 makes a flower-shaped movement above the liquid surface of the culture solution, and further makes the microbial liquid flowing out from the bottom port of the inoculation tube 19 be dispersed in the culture solution of the container body 1 in a flower shape, and finally realizes the flower-shaped dispersion of the first batch of microbial liquid in the culture solution of the container body 1; then in steps S6-S7, only by the linkage of the automatic dispersion component 9 and the fixing and rotating component 4, the flower-shaped dispersion of multiple batches of microbial liquid in the culture solution of the container body 1 can be realized.

[0098] It can be seen from this that compared with the traditional inoculation method, this inoculation device does not require the staff to extract a certain amount of microbial liquid from the storage tank with an inoculator, nor does it require the staff to manually draw the inoculator in a flower shape to disperse the microbial liquid in a flower shape in the culture solution of the container body 1. Instead, it automatically disperses the microbial liquid in a flower shape in the culture solution of the container body 1, and then realizes the flower-shaped dispersion of multiple batches of microbial liquid in the culture solution in a short time, thereby greatly improving the inoculation efficiency of the microbial liquid.

Claims

1. An inoculation device for efficiently inoculating microbial liquid in a large-sized container, characterized in that: It includes a fixing and rotating component (4) arranged on a workbench (3) for fixing and driving a large-sized container to rotate around its own axis, and a servo motor (5) fixed on the bottom surface of the workbench (3). The output shaft of the servo motor (5) penetrates the workbench (3) axially upward, and a main shaft (6) is connected to the extending end. A strip plate (7) is fixedly arranged at the top of the main shaft (6). An automatic lid-removing component (8) for removing the plastic lid (2) of the large-sized container is arranged at the left end of the strip plate (7). The automatic lid-removing component (8) is located directly above the fixing and rotating component (4). An automatic dispersion component (9) for dispersing the microbial liquid in a flower shape in the container body (1) of the large-sized container is arranged at the right end of the strip plate (7). The automatic dispersion component (9) includes a lifting cylinder (10) fixed at the right end of the strip plate (7). The piston rod of the lifting cylinder (10) penetrates the strip plate (7) downward, and a lifting plate (11) is fixedly arranged at the extending end. An L-shaped plate (12) is fixedly arranged on the bottom surface of the left end of the lifting plate (11). An annular ring (13) is fixedly arranged at the end of the L-shaped plate (12). A plurality of arc-shaped grooves (14) arranged at intervals are formed on the inner wall of the central hole of the annular ring (13) along its circumference. A driving motor (15) is fixedly arranged on the bottom surface of the right end of the lifting plate (11). A connecting plate (16) that penetrates the central hole of the annular ring (13) downward is fixedly arranged on the output shaft of the driving motor (15). A movable strip (17) extending to the right slidably penetrates through the lower end of the connecting plate (16). A fixing plate (18), an inoculation tube (19), and a rod member (20) are fixedly arranged on the top surface of the movable strip (17) in sequence from left to right. A spring (21) is fixedly arranged between the fixing plate (18) and the connecting plate (16). The top port of the inoculation tube (19) penetrates the central hole of the annular ring (13) upward and extends above the annular ring (13). A nylon wheel (22) is rotatably installed at the top of the rod member (20). Under the elastic force of the spring (21), the nylon wheel (22) abuts against the bottom of the rightmost arc-shaped groove (14) in the annular ring (13). A storage tank (23) storing the microbial liquid is fixedly arranged on the left end surface of the connecting plate (16). A peristaltic pump (24) is fixedly arranged on the bottom surface of the connecting plate (16). A liquid suction pipe (25) is connected to the liquid suction port of the peristaltic pump (24). The liquid suction pipe (25) penetrates the connecting plate (16) and is connected to the bottom port of the storage tank (23). A liquid discharge pipe (26) is connected to the liquid discharge port of the peristaltic pump (24). The liquid discharge pipe (26) is connected to the top port of the inoculation tube (19). The automatic cover removal assembly (8) includes a cover removal cylinder (33) fixed to the left end of the strip-shaped plate (7). The piston rod of the cover removal cylinder (33) penetrates downward through the strip-shaped plate (7), and an arched frame B is fixed to the extended end. A suction cup B (34) is fixed to the bottom surface of the arched frame B. A plurality of vacuum holes B (35) communicating with its inner cavity are formed on the bottom surface of the suction cup B (34). A vacuum pump B (36) is fixed inside the arched frame B, and the working port of the vacuum pump B (36) is communicated with the inner cavity of the suction cup B (34). Horizontal cylinders (37) are fixed to the top surface of the suction cup B (34) on both its left and right sides. The piston rods of the two horizontal cylinders (37) extend outside the suction cup B (34), and vertical plates are fixed to the extended ends. Lifting plates (38) arranged longitudinally are fixed to the inner end faces of the two vertical plates. Semi-circular holes (39) are formed on the inner end faces of the two lifting plates (38), and the diameter of the semi-circular holes (39) is equal to the outer diameter of the container body (1) of the large-sized container.

2. The inoculation device for efficiently inoculating microbial bacterial liquid in a large-sized container according to claim 1, characterized in that: The axis of the drive motor (15) is coaxial with the axis of the annular ring (13).

3. An inoculation device for efficiently inoculating microbial bacterial liquid in a large-sized container according to claim 2, characterized in that: The arc-shaped grooves (14) are evenly distributed on the inner wall of the annular ring (13).

4. An inoculation device for efficiently inoculating microbial liquid in a large-sized container according to claim 3, characterized in that: A support plate is fixed to the left end face of the connecting plate (16), and the storage tank (23) is fixed to the support plate.

5. The inoculation device for efficiently inoculating microbial liquid in a large-sized container according to claim 4, wherein: The fixing and rotating assembly (4) includes a stepping motor (27) fixed to the tabletop of the workbench (3), an arched frame A fixed to the output shaft of the stepping motor (27). A turntable (28) is fixed to the top of the arched frame A. A positioning hole (29) is formed on the top surface of the turntable (28), and the diameter of the positioning hole (29) is equal to the outer diameter of the container body (1) of the large-sized container. A stepped hole penetrating the bottom surface of the turntable (28) is formed at the bottom of the positioning hole (29). A suction cup A (30) is fixed in the large hole of the stepped hole. The top surface of the suction cup A (30) is flush with the bottom of the positioning hole (29). A plurality of vacuum holes A (31) communicating with its inner cavity are formed on the top surface of the suction cup A (30). A vacuum pump A (32) is fixed inside the arched frame A, and the working port of the vacuum pump A (32) is communicated with the inner cavity of the suction cup A (30) through a pipeline.

6. An inoculation device for efficiently inoculating microbial broth in a large-sized container according to claim 5, characterized in that: A plurality of legs supporting on the ground are fixed to the bottom surface of the workbench (3).

7. An inoculation device for efficiently inoculating microbial liquid in a large-sized container according to claim 6, characterized in that: The inoculation device further includes a controller, which is electrically connected to the servo motor (5), drive motor (15), stepping motor (27), lifting cylinder (10), cover removal cylinder (33), horizontal cylinder (37), peristaltic pump (24), vacuum pump A (32), and vacuum pump B (36) through signal lines.

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