A stem cell culture perfusion device

Abstract

The present invention relates to the field of perfusion devices, and discloses a stem cell culture perfusion device, comprising a culture component, the culture component comprising a cell culture tank, a first connection portion with a concave cross-section and a trapezoidal cross-section is arranged on the top of the cell culture tank, a first thread is arranged on the outer surface of the first connection portion, and a perfusion component is arranged on the top of the cell culture tank; the perfusion component comprises a barrel, a material injection port is arranged on the top of the barrel, a second connection portion is arranged on the bottom of the barrel, a second thread is arranged on the surface of the second connection portion, the second thread and the first thread can be threadedly connected with each other, and guide components are arranged on both sides of the perfusion component. The present invention does not require an external power supply or a complex control system, and uses a mechanical energy storage method to achieve stable operation within a period of time, greatly improves the automation and stability of the culture, and provides a more reliable and energy-saving solution for stem cell culture.

Description

Technical Field

[0001] The invention belongs to the field of perfusion devices, in particular to a stem cell culture perfusion device. Background Art

[0002] Stem cells are a type of cell that has unlimited or immortal self-renewal capacity and can produce at least one type of highly differentiated progeny cells.

[0003] Serum-free stem cell culture, as an important breakthrough in modern cell culture technology, represents the direction of future medical development. Compared with traditional serum culture methods, serum-free culture has higher controllability and less risk of immune response while reducing costs. Serum, as a complex component in the culture medium, can provide the nutrients and growth factors required by stem cells, but its high cost and possible batch differences make it limited in large-scale applications. Therefore, serum-free stem cell culture has become a hot topic of research. By optimizing the composition of the culture medium and adding synthetic or recombinant factors, efficient directional differentiation and large-scale culture of stem cells can be achieved, and it has broad application potential, especially in clinical treatment, drug development, and tissue engineering.

[0004] Existing cell culture perfusion devices usually need to discharge the waste liquid generated by the culture in the middle and later stages of the culture. However, the power structure of the existing device for discharging the waste liquid is usually electric, which has certain limitations in use. Therefore, a stem cell culture perfusion device is proposed. Summary of the invention

[0005] In order to solve the problems raised in the above background technology, the present invention provides the following technical solutions: a stem cell culture perfusion device, comprising:

[0006] A culture component, the culture component comprising a cell culture tank, a first connecting portion with a concave shape and a trapezoidal cross section is arranged on the top of the cell culture tank, a first thread is arranged on the outer surface of the first connecting portion, and a perfusion component is arranged on the top of the cell culture tank;

[0007] A perfusion component comprises a barrel, wherein a material injection port is arranged at the top of the barrel, a second connection part is arranged at the bottom of the barrel, a second thread is arranged on the surface of the second connection part, and the second thread and the first thread can be threadedly connected with each other, and guide components are arranged on both sides of the perfusion component, and the guide components are used to guide the perfusion component to prevent rotation when it is vertically installed, when the second connection part is inserted into the first connection part, it is guided by the guide component to move axially without rotating, and the axial movement of the second connection part drives the first connection part to rotate around its axis through the meshing action of the thread, thereby driving the cell culture tank to rotate;

[0008] The base has an energy storage component and a peristaltic pump disposed therein. The energy storage component has a transmission shaft connected to the bottom of the cell culture tank. The peristaltic pump includes a rotating shaft, which is transmission-connected to the energy storage component.

[0009] Furthermore, the energy storage component includes a first gear, an energy charging gear, a first composite gear, a second composite gear, an energy releasing gear and a clockwork mechanism. The first gear is fixedly sleeved on the transmission shaft and meshed with the large toothed disc of the first composite gear. The energy charging gear is meshed with the large toothed disc of the first composite gear. The energy charging gear is meshed with the small toothed disc of the second composite gear. A clockwork shaft is fixedly connected to the center of the second composite gear, and the clockwork shaft is connected to the spring in the clockwork mechanism.

[0010] Furthermore, a third gear is fixedly connected to the rotating shaft, and the third gear is meshedly connected to the first gear via a transmission gear.

[0011] Furthermore, the guide assembly includes a vertical rod, a flat plate and a guide groove arranged on the side wall of the flat plate, the vertical rod is arranged at the top of the base, a slider is arranged on the top of the vertical rod, a slide rail is arranged at the bottom of the flat plate, the slide rail and the slider are slidably connected, a guide block is arranged on the side wall of the barrel, the guide block is slidably connected to the guide groove, and the two flat plates are connected by a snap-on structure.

[0012] Furthermore, limit blocks are respectively arranged at both ends of the slide rail.

[0013] Furthermore, a support rod is arranged between the bottom of the first connecting part and the bottom wall of the inner cavity of the cell culture tank, a slip ring is slidably connected between the upper edge of the side wall of the first connecting part and the upper edge of the inner wall of the cell culture tank, a through hole is opened on the slip ring, and the input end of the peristaltic pump is connected to a suction pipe, the suction pipe passes through the side wall of the base, and the suction pipe passes through the through hole and is inserted into the cell culture tank.

[0014] Furthermore, a pipe rack is provided on the upper wall of the base, and a pipe clamp is provided on the upper end of the pipe rack, and the pipe clamp is used to clamp the suction pipe.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The stem cell culture perfusion device of the present invention is cleverly designed with a mechanical structure. After the perfusion component is installed, it drives the energy storage component to store kinetic energy. The energy storage system releases energy in the middle and late stages of cell culture through a clockwork mechanism to drive the cell culture tank to rotate. During the rotation process, the waste liquid at the bottom of the culture tank is slightly shaken, which improves the uniformity of the waste liquid, helps to prevent the waste liquid from being deposited at the bottom of the tank, and ensures the uniformity of the cell culture environment. At the same time, the power released by the clockwork is connected to the peristaltic pump through the transmission shaft, and the peristaltic pump is started to further enhance the waste liquid removal effect. The continuous operation of the peristaltic pump can effectively suck out the waste liquid in the cell culture tank, avoid cell damage, and ensure the timely replacement of the culture solution and the cleanliness of the cell growth environment. The entire process does not require an external power supply or a complex control system, and uses a mechanical energy storage method to achieve stable operation over a period of time, which greatly improves the automation and stability of the culture, and provides a more reliable and energy-saving solution for stem cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0018] Figure 1 It is a front view structural schematic diagram of the present invention as a whole;

[0019] Figure 2 It is a schematic diagram of the cross-sectional structure of the present invention;

[0020] Figure 3 It is a schematic diagram of the cross-sectional structure of the culture component part of the present invention;

[0021] Figure 4 It is a schematic diagram of the cross-sectional structure of the perfusion component of the present invention;

[0022] Figure 5 This is a schematic structural diagram of the bottom portion of the perfusion assembly of the present invention;

[0023] Figure 6 It is a schematic diagram of the top view of the guide assembly part of the present invention;

[0024] Figure 7 It is a schematic diagram of the top view of the culture component part of the present invention;

[0025] In the figure: 1. culture component; 101. cell culture tank; 102. first connection part; 103. first thread; 104. support rod; 105. slip ring; 2. perfusion component; 201. barrel; 202. injection port; 203. second connection part; 204. second thread; 205. guide block; 3. base; 4. energy storage component; 401. first gear; 402. charging gear; 403. first composite gear; 404. second composite gear; 405. spring shaft; 406. spring mechanism; 407. energy release gear; 408. transmission shaft; 5. peristaltic pump; 501. rotating shaft; 502. third gear; 503. transmission gear; 6. guide component; 601. vertical rod; 602. plate; 603. guide groove; 604. slide rail; 7. suction pipe; 8. pipe rack; 9. pipe clamp. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0027] Depend on Figure 1-7 The present invention provides a stem cell culture perfusion device, comprising:

[0028] The culture component 1 comprises a cell culture tank 101, a first connecting portion 102 having a concave shape and a trapezoidal cross section is arranged on the top of the cell culture tank 101, a first thread 103 is arranged on the outer surface of the first connecting portion 102, and a perfusion component 2 is arranged on the top of the cell culture tank 101;

[0029] The perfusion component 2 includes a barrel 201, a material injection port 202 is provided at the top of the barrel 201, a second connection part 203 is provided at the bottom of the barrel 201, a second thread 204 is provided on the surface of the second connection part 203, and the second thread 204 and the first thread 103 can be threadedly connected with each other, and guide components 6 are provided on both sides of the perfusion component 2. The guide component 6 is used to guide the perfusion component 2 to not rotate when it is vertically installed. When the second connection part 203 is inserted into the first connection part 102, it is guided by the guide component 6 to move axially without rotating. The axial movement of the second connection part 203 drives the first connection part 102 to rotate around the first connection part 102 through the thread engagement. Its axis rotates, thereby driving the cell culture tank 101 to rotate; the cell culture tank 101 contains a solution containing stem cells, and the barrel 201 contains a culture solution required for stem cell culture. When the barrel 201 is installed on the cell culture tank 101, the gravity of the barrel 201 itself can be used as a driving force to drive the cell culture tank 101 to rotate. The two sides of the barrel 201 cannot rotate due to the guidance of the guide component 6, and then the first connecting part 102 is driven to rotate around its axis through the threaded engagement, thereby driving the cell culture tank 101 to rotate, and by driving the transmission shaft 408 to rotate, the clockwork mechanism 406 set in the energy storage component 4 is tightened to store energy;

[0030] The base 3 has an energy storage component 4 and a peristaltic pump 5 disposed therein. The energy storage component 4 has a transmission shaft 408 connected to the bottom of the cell culture tank 101 . The peristaltic pump 5 includes a rotating shaft 501 , which is transmission-connected to the energy storage component 4 .

[0031] The energy storage assembly 4 includes a first gear 401, an energy charging gear 402, a first composite gear 403, a second composite gear 404, an energy releasing gear 407 and a clockwork mechanism 406. The first gear 401 is fixedly sleeved on a transmission shaft 408 and meshed with a large toothed disc of the first composite gear 403. The energy charging gear 402 is meshed with a large toothed disc of the first composite gear 403. The energy charging gear 402 is meshed with a small toothed disc of the second composite gear 404. A clockwork shaft 405 is fixedly connected to the center of the second composite gear 404. , the spring shaft 405 is connected to the spring in the spring mechanism 406; when the barrel 201 is installed, the charging gear 402 can mesh with the first composite gear 403 and the second composite gear 404, thereby tightening the spring mechanism 406; when the spring mechanism 406 is released, the charging gear 402 is separated from the second composite gear 404, and the energy releasing gear 407 is meshed with the first composite gear 403 and the second composite gear 404, thereby releasing the energy stored in the spring mechanism 406, thereby driving the transmission shaft 408 to rotate.

[0032] A third gear 502 is fixedly connected to the rotating shaft 501, and the third gear 502 is meshed with the first gear 401 via a transmission gear 503. When the clockwork mechanism 406 is released, the energy storage component 4 transmits the action to the third gear 502 via the transmission gear 503, and then drives the peristaltic pump 5 to work through the rotating shaft 501 to suck out the waste liquid in the cell culture tank 101.

[0033] The guide assembly 6 includes a vertical rod 601, a flat plate 602, and a guide groove 603 arranged on the side wall of the flat plate 602. The vertical rod 601 is arranged on the top of the base 3. A slider is arranged on the top of the vertical rod 601. A slide rail 604 is arranged on the bottom of the flat plate 602. The slide rail 604 and the slider are slidably connected. A guide block 205 is arranged on the side wall of the barrel 201. The guide block 205 is slidably connected to the guide groove 603. The two flat plates 602 are connected by a snap-fit ​​structure. When the barrel 201 is installed on the top of the cell culture tank 101, The guide blocks 205 on both sides are installed in alignment with the guide grooves 603, which can prevent the barrel 201 from rotating. After a certain period of stem cell culture, the plate 602 is separated to both sides by opening the buckle structure to release the barrel 201. Since the barrel 201 and the cell culture tank 101 are an integral structure when installed, when the barrel 201 is released, the energy storage component 4 can release the stored energy, drive the transmission shaft 408 to rotate, and then drive the cell culture tank 101 to rotate and drive the peristaltic pump 5 to start working.

[0034] Limiting blocks are respectively arranged at both ends of the slide rail 604 , and the setting of the limiting blocks can prevent the plate 602 from slipping off the top of the vertical rod 601 .

[0035] A support rod 104 is arranged between the bottom of the first connecting part 102 and the bottom wall of the inner cavity of the cell culture tank 101, and a slip ring 105 is slidably connected between the upper edge of the side wall of the first connecting part 102 and the upper edge of the inner wall of the cell culture tank 101. A through hole is opened on the slip ring 105, and the input end of the peristaltic pump 5 is connected to the suction pipe 7, which passes through the side wall of the base 3 and is inserted into the cell culture tank 101 through the through hole.

[0036] A tube rack 8 is provided on the upper wall of the base 3, and a tube clamp 9 is provided on the upper end of the tube rack 8. The tube clamp 9 is used to clamp the suction tube 7. The tube clamp 9 stably clamps the suction tube 7. When the cell culture tank 101 rotates, the slip ring 105 can rotate around the axis to prevent the suction tube 7 from being entangled with the rotation of the cell culture tank 101.

[0037] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0038] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A stem cell culture perfusion device, characterized in that: include: A culture component (1), the culture component (1) comprising a cell culture tank (101), a first connecting portion (102) which is concave and has a trapezoidal cross section is arranged on the top of the cell culture tank (101), a first thread (103) is arranged on the outer surface of the first connecting portion (102), and a perfusion component (2) is arranged on the top of the cell culture tank (101); A perfusion assembly (2), comprising a barrel (201), wherein a material injection port (202) is arranged at the top of the barrel (201), and a second connection portion (203) is arranged at the bottom of the barrel (201), and a second thread (204) is arranged on the surface of the second connection portion (203), and the second thread (204) and the first thread (103) can be threadedly connected to each other, and guide assemblies (6) are arranged on both sides of the perfusion assembly (2), and the guide assemblies (6) are used to guide the perfusion assembly (2) to prevent rotation when the perfusion assembly (2) is installed vertically, and when the second connection portion (203) is inserted into the first connection portion (102), it is guided by the guide assemblies (6) to move axially without rotating, and the axial movement of the second connection portion (203) drives the first connection portion (102) to rotate around its axis through the meshing action of the threads, thereby driving the cell culture tank (101) to rotate; A base (3), wherein an energy storage component (4) and a peristaltic pump (5) are arranged in the base (3), wherein the energy storage component (4) has a transmission shaft (408), wherein the transmission shaft (408) is connected to the bottom of the cell culture tank (101), and the peristaltic pump (5) comprises a rotating shaft (501), wherein the rotating shaft (501) is in transmission connection with the energy storage component (4).

2. A stem cell culture perfusion device according to claim 1, characterized in that: The energy storage component (4) comprises a first gear (401), an energy charging gear (402), a first composite gear (403), a second composite gear (404), an energy releasing gear (407) and a clockwork mechanism (406); the first gear (401) is fixedly sleeved on a transmission shaft (408) and meshedly connected with a large toothed disc of the first composite gear (403); the energy charging gear (402) is meshedly connected with a large toothed disc of the first composite gear (403); the energy charging gear (402) is meshedly connected with a small toothed disc of the second composite gear (404); a clockwork shaft (405) is fixedly connected to the center of the second composite gear (404); the clockwork shaft (405) is connected with a clockwork spring in the clockwork mechanism (406).

3. A stem cell culture perfusion device according to claim 2, characterized in that: A third gear (502) is fixedly connected to the rotating shaft (501), and the third gear (502) is meshedly connected to the first gear (401) via a transmission gear (503).

4. A stem cell culture perfusion device according to claim 3, characterized in that: The guide assembly (6) comprises a vertical rod (601), a flat plate (602) and a guide groove (603) arranged on the side wall of the flat plate (602); the vertical rod (601) is arranged on the top of the base (3); a slider is arranged on the top of the vertical rod (601); a slide rail (604) is arranged on the bottom of the flat plate (602); the slide rail (604) and the slider are slidably connected; a guide block (205) is arranged on the side wall of the barrel (201); the guide block (205) is slidably connected to the guide groove (603); and the two flat plates (602) are connected via a snap-fit ​​structure.

5. A stem cell culture perfusion device according to claim 4, characterized in that: Limiting blocks are respectively arranged at both ends of the slide rail (604).

6. A stem cell culture perfusion device according to claim 5, characterized in that: A support rod (104) is provided between the bottom of the first connecting portion (102) and the bottom wall of the inner cavity of the cell culture tank (101); a slip ring (105) is slidably connected between the upper edge of the side wall of the first connecting portion (102) and the upper edge of the inner wall of the cell culture tank (101); a through hole is provided on the slip ring (105); an input end of the peristaltic pump (5) is connected to a suction pipe (7); the suction pipe (7) passes through the side wall of the base (3); and the suction pipe (7) passes through the through hole and is inserted into the cell culture tank (101).

7. A stem cell culture perfusion device according to claim 6, characterized in that: A pipe rack (8) is provided on the upper wall of the base (3), and a pipe clamp (9) is provided on the upper end of the pipe rack (8), and the pipe clamp (9) is used to clamp the suction pipe (7).

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