Application of qCTS11.4 gene in regulating cold tolerance in rice
By knocking out the cold tolerance-related gene qCTS11.4 in rice, the problem of rice being sensitive to low temperature stress was solved, which significantly improved the cold tolerance of rice and improved its cold tolerance.
Patent Information
- Application Number
- CN202411749946.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-02
- Publication Date
- 2025-04-04
AI Technical Summary
Rice is sensitive to low-temperature stress, which leads to impeded growth of seedlings and physiological metabolic disorders. In severe cases, it may cause yellowing and withering of leaves, resulting in a significant decline in yield and quality.
Through gene editing technology, the cold tolerance-related gene qCTS11.4 found in rice was knocked out, thereby improving the cold tolerance of rice.
After knocking out the qCTS11.4 gene, the cold tolerance of genetically modified rice is significantly improved, and the survival rate can be higher under low temperature conditions, improving the cold tolerance of rice.
Smart Images
Figure CN119752986A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of rice molecular breeding, and in particular to application of qCTS11.4 gene in regulating cold tolerance of rice. Background Art
[0002] Rice (Oryza sativa L.) is the most important staple food crop in my country, and more than half of the population relies on rice as their staple food. Since rice originates from tropical and subtropical regions, it is more sensitive to low temperature stress than other crops. Low temperature stress can cause growth stagnation and physiological metabolic disorders in rice seedlings. In severe cases, it may cause yellowing, withering, or even death of rice leaves, resulting in a significant decrease in yield and quality. Therefore, conducting research on rice cold tolerance and exploring excellent cold tolerance genes are of great theoretical and practical significance for promoting the increase and stability of rice production in my country and ensuring national food security.
[0003] In this study, we predicted a rice cold-tolerance-related gene qCTS11.4 and constructed transgenic materials to verify and analyze the gene, providing a theoretical basis and genetic resources for breeding excellent rice varieties that can resist low temperature damage. Summary of the invention
[0004] The purpose of the present invention is to provide an application of the qCTS11.4 gene in regulating the cold tolerance of rice. In the study, a gene qCTS11.4 that affects the cold tolerance of rice was identified; further research found that when the qCTS11.4 gene was knocked out, the cold tolerance of transgenic rice could also be improved, so as to solve the problems raised in the above background technology.
[0005] In order to achieve the above object, the present invention adopts the following technical solutions:
[0006] The invention provides application of qCTS11.4 gene in regulating cold tolerance of rice.
[0007] The present invention also provides a method for improving the cold tolerance of rice, comprising the step of knocking out the qCTS11.4 gene from the genome of a starting rice line.
[0008] Preferably, the qCTS11.4 gene is knocked out from the genome of the starting rice line by gene editing.
[0009] Compared with the prior art, the present invention has the following beneficial effects:
[0010] The present invention discovered a new cold tolerance-related gene qCTS11.4, which provided a theoretical basis and gene resources for rice cold tolerance improvement and breeding, and can be used for transgenic breeding. BRIEF DESCRIPTION OF THE DRAWINGS
[0011] Figure 1 CRISPR / Cas9 knockout of qCTS11.4 increases cold tolerance in rice seedlings. A is a schematic diagram of CRISPR / Cas9 knockout of qCTS11.4, with the gene structure of qCTS11.4 above and the sequence variation of the knockout family below; B is the cold tolerance phenotype of the qCTS11.4 knockout family and NIP; C is the survival rate of the qCTS11.4 knockout family and NIP under 4℃ cold treatment; significance is obtained by two-tailed t test, error bars, sem, number of replicates n=3. DETAILED DESCRIPTION
[0012] In order to further explain the technical solution of the present invention, it is described in detail below through specific embodiments.
[0013] See also Figure 1 The present invention provides the use of qCTS11.4 gene in regulating the cold tolerance of rice.
[0014] The present invention also provides a method for improving the cold tolerance of rice, comprising the step of knocking out the qCTS11.4 gene from the genome of a starting rice line.
[0015] Among them, the qCTS11.4 gene was knocked out from the genome of the starting rice line through gene editing.
[0016] CRISPR / Cas9 knockout of qCTS11.4 increases cold tolerance in rice seedlings.
[0017] In order to study the biological function of qCTS11.4, we used the CRISPR / Cas9 dual-target knockout system to mutate qCTS11.4 in the parental material Nipponbare background to obtain a transgenic knockout family of qCTS11.4 ( Figure 1 A). Through sequencing and comparison, we obtained two different types of transgenic knockout homozygous materials of qCTS11.4, qCTS11.4-1 (+2bp) and qCTS11.4-2 (-41bp), both of which resulted in frameshift mutation and loss of function of qCTS11.4.
[0018] We further subjected the qCTS11.4 knockout family and the wild-type control Nipponbare seedlings to a 4°C cold treatment for 72 hours, and examined the survival rate of the seedlings after 14 days of recovery. After the recovery culture was completed, the survival rate was statistically analyzed and the cold tolerance was evaluated. The results showed that the survival rate of the qCTS11.4-1 and qCTS11.4-2 mutant materials was higher than that of the wild-type Nipponbare ( Figure 1 B and Figure 1 C), indicating that knocking out qCTS11.4 can significantly increase the cold tolerance of rice at the seedling stage.
[0019] The above experiments show that when the qCTS11.4 gene is knocked out, the resulting transgenic rice lines also have higher cold tolerance.
[0020] The invention provides a novel cold tolerance related gene qCTS11.4, which provides a theoretical basis and gene resources for rice cold tolerance improvement and breeding, and can be used for hybrid breeding and transgenic breeding.
[0021] The above description is only a preferred example of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. Application of qCTS11.4 gene in regulating cold tolerance of rice.
2. A method for improving cold tolerance of rice, characterized in that: The method comprises the steps of knocking out the qCTS11.4 gene from the genome of the starting rice line.
3. A method for improving cold tolerance of rice according to claim 2, characterized in that: The qCTS11.4 gene was knocked out from the genome of the starting rice line by gene editing.