Method for restoring ecologically fragile area by utilizing algae-moss-grass symbiosis

Abstract

The invention discloses a method for restoring an ecologically fragile area by utilizing algae-moss-grass symbiosis, and relates to the technical field of ecosystem restoration, and the method comprises the following steps: S1, performing deep ploughing and soil turning on soil of a to-be-treated area, covering the soil with muck with the thickness of 4-6cm, and performing water supply irrigation on the soil by adopting a surface irrigation, common spray irrigation or micro-irrigation mode, the soil is observed to be just completely wet; s2, uniformly scattering herbaceous plant seeds into the residue soil, and slightly stirring the shallow residue soil with a shovel to cover the seeds; s3, the moss spore powder is evenly spread on the surface of the muck through a screen; s4, performing third-stage expanding culture on algae to obtain an algae culture solution after third-stage expanding culture; uniformly mixing the algae culture solution subjected to the third-stage expanding culture and the modified clay mineral, and applying the mixture to the surface of the muck; after planting is completed, water is regularly supplied to the soil in a ground irrigation mode or a common sprinkling irrigation mode or a micro-irrigation mode. The planting method provided by the invention has an excellent soil remediation effect.

Description

Technical Field

[0001] The present application relates to the field of ecosystem restoration technology, and in particular to a method for restoring ecologically fragile areas using algae-moss-grass symbiosis. Background Art

[0002] Desertification, a global environmental problem, is devouring precious land resources at an unprecedented rate, posing a serious threat to the sustainable development of human society. The causes of desertification are complex and diverse, including vegetation destruction, water resource depletion, overcultivation and unreasonable land use. These factors work together to lead to land degradation and ecosystem imbalance. To meet this challenge, scientists continue to explore and practice various ecological restoration technologies in order to restore the productivity and ecological functions of damaged land.

[0003] Biological crust restoration technology can be used as a green and environmentally friendly means to transform various harsh environments and poor soils. Thanks to the specific structure, habits, secretions, etc. of the organisms in the crust, it can have a positive impact on soil organic matter content, porosity, bulk density, enzyme activity and other indicators.

[0004] However, the current practice of biocrust restoration technology is mainly limited to the intervention of a single species. Although this method can improve soil conditions to a certain extent, its limitations are becoming increasingly prominent. It is difficult to fully adapt to the diverse desertification environment and it is difficult to effectively deal with large-scale and high-intensity soil degradation problems. Therefore, there is an urgent need for a method that can effectively improve complex and diverse soil conditions and achieve effective management and ecological restoration of desertified land. Summary of the invention

[0005] In order to solve the above problems, the present application provides a method for restoring ecologically fragile areas using algae-moss-grass symbiosis.

[0006] The present application provides a method for restoring ecologically fragile areas by using algae-moss-grass symbiosis, which adopts the following technical solution: A method for restoring ecologically fragile areas using algae-moss-grass symbiosis, comprising the following steps: S1. Deeply plow the soil in the area to be treated and cover it with soil with a thickness of 4-6 cm. Water the soil by surface irrigation, ordinary sprinkler irrigation or micro-irrigation until the soil is completely moistened. S2. Sprinkle the herb seeds evenly into the soil and gently stir the shallow soil with a shovel to cover the seeds; S3. Use a sieve to evenly spread the moss spore powder on the surface of the soil; S4. After the algae are expanded three times, a three-stage expanded algae culture solution is obtained; after the three-stage expanded algae culture solution and the modified clay mineral are evenly mixed, they are applied to the surface of the slag soil; after the planting is completed, the soil is regularly irrigated by surface irrigation, ordinary sprinkler irrigation or micro-irrigation.

[0007] Preferably, the herbaceous plant seeds are ryegrass seeds; the sowing rate of the ryegrass seeds is 4860 seeds / m 2 .

[0008] Preferably, the algae is one of Microcoleus, Nostoc, and Monochamus.

[0009] Preferably, the amount of algae culture solution added is 1-10 g / m 2 The modified clay mineral is added in an amount of 10-100 g / m 2 .

[0010] Preferably, the modified clay mineral is prepared from the following raw materials in parts by weight: 10-20 parts of water, 0.2-0.4 parts of hexadecyltrimethylammonium chloride, and 0.8-1.6 parts of clay mineral.

[0011] Preferably, the clay mineral is a mixture of kaolin and montmorillonite in a mass ratio of 1:0.5-1.5.

[0012] Preferably, the method for preparing the modified clay mineral comprises the following steps: After mixing hexadecyltrimethylammonium chloride and water, stir until dissolved, heat to 60-70° C., add clay mineral, stir and mix to react for 1-3 hours, filter, and vacuum dry to obtain modified clay mineral.

[0013] Preferably, the moss spore powder is one of sand moss spore powder, large gray moss spore powder, and cluster true moss spore powder; the amount of the moss spore powder added is 10-100g / m 2 .

[0014] Preferably, the method for three-stage expansion of algae culture solution comprises the following steps: S1. Add the algae to the sterilized culture medium for primary expansion, mix well and place in a light constant temperature incubator, culture for 15 days at 3000LUX, 25℃, and 12h light-dark cycle, and shake once a day to prevent hardening or wall adhesion; S2. Add the mixed algae liquid obtained from the primary expansion to the sterilized culture medium for secondary expansion, place in a light constant temperature incubator, culture for 15 days at 3000LUX, 25℃, 12h light-dark cycle, and shake gently every day; S3. The third-level expansion culture is carried out in an algae culture vessel. After the algae culture vessel is sterilized with ultraviolet light for 12 hours, the sterilized culture medium is added, and then the mixed algae solution obtained from the second-level expansion culture is added. The middle lamp tube and the lower pump air valve are turned on for cultivation. The ventilation volume at the bottom is 8L per cubic minute. The culture vessel is gently shaken every day, and the culture medium is replenished after 15 days, and then cultured for another 15 days.

[0015] Preferably, the herbaceous plant seeds are ryegrass, and the sowing rate is 4860 seeds / m 2 The moss spore powder is sand moss spore powder, and the addition amount is 100g / m 2 The algae culture solution is a three-stage expanded Nostoc culture solution, and the addition amount is 10g / m 2 The modified clay mineral addition amount is 100g / m 2 .

[0016] In summary, the present application includes at least one of the following beneficial technical effects: 1. The algae-moss-grass symbiotic restoration method provided in this application breaks through the single species selection mode in the biological crust process, and effectively improves the soil restoration effect through the symbiosis of algae-moss-grass and synergistic enhancement; Figure 1 As shown, after the original soil is improved by algal crusts and lichen crusts, the organic carbon content, water content, soil particle size, pH and other conditions become suitable for the growth of higher plants. At this time, mosses will begin to take root and multiply locally to form moss crusts; and after the appearance of mosses, the biomass will increase over time, and the crust coverage will gradually expand. The combined effect of mosses, algae, fungi and other soil microorganisms on the soil provides suitable conditions for the growth of herbaceous plants, thereby enabling them to grow vigorously; the restoration method provided in the present application has strong adaptability to the environment, can survive and function under harsh soil conditions, and is particularly suitable for areas with severe desertification and poor soil.

[0017] 2. This application uses a compound of kaolin and montmorillonite as clay minerals, and uses hexadecyltrimethylammonium chloride for modification, which effectively improves the water retention of the clay minerals. It is mixed with algae culture medium and effectively improves the organic carbon content, water content, soil particle size, pH and other conditions in the soil, making it more suitable for plant growth, and effectively promotes the formation and rapid development of algae crust, thereby accelerating the efficiency of ecological restoration and improving the restoration effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the principle of the method of using algae-moss-grass symbiosis to restore ecologically fragile areas in this application; Figure 2 is the germination rate of ryegrass under the planting methods of Examples 1-9 and Comparative Example 1 of the present application; Figure 3The soil pH conditions under the planting methods of Examples 1-9 and Comparative Example 1 of the present application; Figure 4 It is the soil available nitrogen content under the planting methods of Examples 1-9 and Comparative Example 1 of the present application; Figure 5 It is the soil available potassium content under the planting methods of Examples 1-9 and Comparative Example 1 of the present application; Figure 6 It is the soil available phosphorus content under the planting methods of Examples 1-9 and Comparative Example 1 of the present application; Figure 7 is the organic matter content in the soil under the planting methods of Examples 1-9 and Comparative Example 1 of the present application; Figure 8 is the activity of soil catalase under the planting methods of Examples 1-9 of the present application and Comparative Example 1; Fig. 9 is the soil alkaline phosphatase activity under the planting methods of Examples 1-9 and Comparative Example 1 of the present application; Fig.10 It is the soil sucrase activity under the planting methods of Examples 1-9 and Comparative Example 1 of the present application. DETAILED DESCRIPTION

[0019] The present application is further described in detail below with reference to the embodiments.

[0020] Preparation Example 1) The method for preparing the modified clay mineral provided in the present application comprises the following steps: Preparation Example 1.1 0.2 g of hexadecyltrimethylammonium chloride and 10 g of water were mixed, stirred until dissolved, heated to 60°C, 0.8 g of clay mineral was added, stirred and mixed for 1 h, filtered, and vacuum dried to obtain a modified clay mineral; the clay mineral used in this preparation example was a mixture of kaolin and montmorillonite in a mass ratio of 1:0.5.

[0021] Preparation Example 1.2 0.3 g of hexadecyltrimethylammonium chloride and 15 g of water were mixed, stirred until dissolved, heated to 65° C., 1.2 g of clay mineral was added, stirred and mixed for 2 h, filtered, and vacuum dried to obtain a modified clay mineral; the clay mineral used in this preparation example was a mixture of kaolin and montmorillonite in a mass ratio of 1:1.

[0022] Preparation Example 1.3 0.4 g of hexadecyltrimethylammonium chloride and 20 g of water were mixed, stirred until dissolved, heated to 70°C, 1.6 g of clay mineral was added, stirred and reacted for 3 h, filtered, and vacuum dried to obtain a modified clay mineral; the clay mineral used in this preparation example was a mixture of kaolin and montmorillonite in a mass ratio of 1:1.5.

[0023] The present application preferably uses the modified clay mineral prepared by the preparation method of Preparation Example 2 in the method of algae-moss-grass symbiotic restoration of ecologically fragile areas.

[0024] 2) The algae culture solution provided in this application is obtained after three-stage expansion of algae, which specifically includes the following steps: Preparation Example 2.1 S1. Add 0.17g BG11 medium and 100mL distilled water in a 200mL conical flask at a ratio of 1.70g / L and mix thoroughly. After sterilization at 121℃ high pressure steam for 15min, obtain the sterilized BG11 medium for standby use; set aside; after natural cooling, take 1mL of sheathed Microcoleus on the clean bench and add it to the sterilized BG11 medium for standby use for primary expansion culture. After mixing, place it in a light constant temperature incubator and culture it for 15 days at 3000LUX, 25℃, and 12h light-dark alternation cycle. During this period, shake it once a day to prevent hardening or wall adhesion; S2. Take 10 mL of the mixed algae liquid obtained from the first-stage expansion and add it to 500 mL of sterilized BG11 medium for expansion. Place it in a light-controlled constant temperature incubator and culture it for 15 days at 3000 LUX, 25°C, and a 12-hour light-dark cycle, with gentle shaking every day. S3. The third-level expansion culture is carried out in an algae culture vessel with a volume of 5L, an air inlet at the bottom for pumping air, and a sealed lamp connected to the top cover for providing light for the algae. The cover can be removed for inoculation and liquid addition; after the entire culture vessel is sterilized with ultraviolet light for 12 hours, 3L of BG11 culture medium prepared and sterilized for standby use is added, and the mixed algae liquid obtained from the second-level expansion culture is added. The middle lamp and the lower pump valve are opened for cultivation, and the ventilation volume at the bottom is 8L per cubic minute. The culture vessel is gently shaken every day, and the culture medium is replenished once after 15 days, and then cultured for another 15 days; the culture liquid of Microcoleus scaly is obtained.

[0025] Preparation Example 2.2 S1. Add 0.17g BG11 medium and 100mL distilled water to a 200mL conical flask at a ratio of 1.70g / L and mix thoroughly. After sterilization at 121℃ high pressure steam for 15min, obtain the sterilized BG11 medium for standby use; set aside; after natural cooling, take 1mL of monophyll on the clean bench and add it to the sterilized BG11 medium for standby use for primary expansion culture. After mixing, place it in a light constant temperature incubator and culture it for 15 days at 3000LUX, 25℃, and 12h light-dark alternation cycle. During this period, shake it once a day to prevent hardening or wall adhesion; S2. Take 10 mL of the mixed algae liquid obtained from the first-stage expansion and add it to 500 mL of sterilized BG11 medium for expansion. Place it in a light-controlled constant temperature incubator and culture it for 15 days at 3000 LUX, 25°C, and a 12-hour light-dark cycle, with gentle shaking every day. S3. The third-level expansion culture is carried out in an algae culture vessel with a volume of 5L, an air inlet at the bottom for pumping air, and a sealed lamp connected to the top cover for providing light for the algae. The cover can be removed for inoculation and liquid addition. After the culture vessel is sterilized with ultraviolet light for 12 hours, 3L of BG11 culture medium prepared and sterilized for standby use is added, and the mixed algae liquid obtained from the second-level expansion culture is added. The middle lamp and the lower pump valve are opened for cultivation, and the ventilation volume at the bottom is 8L per cubic minute. The culture vessel is gently shaken every day, and the culture medium is replenished once after 15 days, and then cultured for another 15 days; the culture liquid of monophyly algae is obtained.

[0026] Preparation Example 2.3 S1. Add 0.17g BG11 medium and 100mL distilled water in a 200mL conical flask at a ratio of 1.70g / L and mix thoroughly. After sterilization at 121℃ high pressure steam for 15min, obtain the sterilized BG11 medium for standby use; set aside; after natural cooling, take 1mL of Nostoc on the clean bench and add it to the sterilized BG11 medium for standby use for primary expansion culture. After mixing, place it in a light constant temperature incubator and culture it for 15d at 3000LUX, 25℃, and 12h light-dark alternation cycle. During this period, shake it once a day to prevent hardening or wall adhesion; S2. Take 10 mL of the mixed algae liquid obtained from the first-stage expansion and add it to 500 mL of sterilized BG11 medium for expansion. Place it in a light-controlled constant temperature incubator and culture it for 15 days at 3000 LUX, 25°C, and a 12-hour light-dark cycle, with gentle shaking every day. S3. The third-level expansion culture is carried out using an algae culture vessel with a volume of 5L, an air inlet at the bottom for pumping air, and a sealed lamp connected to the top cover for providing light for the algae. The cover can be removed for inoculation and liquid addition; after the entire culture vessel is sterilized with ultraviolet light for 12 hours, 3L of BG11 culture medium prepared and sterilized for standby use is added, and the mixed algae liquid obtained from the second-level expansion culture is added. The middle lamp and the lower pump valve are opened for cultivation, and the ventilation volume at the bottom is 8L per cubic minute. The culture vessel is gently shaken every day, and the culture medium is replenished once after 15 days, and then cultured for another 15 days; the culture fluid of Nostoc algae is obtained.

[0027] The technical solution of the present invention is further illustrated by the following embodiments.

[0028] Example Example 1

[0029] A method for restoring ecologically fragile areas using algae-moss-grass symbiosis, comprising the following steps: S1. Deeply plow the soil in the area to be treated and cover it with soil to a thickness of 5 cm. Water the soil by surface irrigation, ordinary sprinkler irrigation or micro-irrigation until the soil is completely moistened. S2. Ryegrass seeds are 4860 grains / m 2 Sprinkle evenly into the soil and gently stir the shallow soil with a shovel to cover the seeds; S3. Use a sieve to press the sand moss spore powder to 10g / m 2 Spread evenly on the surface of the soil; S4. The sheathed Microcoleus was subjected to three-stage expansion to obtain a three-stage expansion culture solution of the sheathed Microcoleus; the three-stage expansion culture solution of the sheathed Microcoleus was added at an amount of 1 g / m 2 , the amount of modified clay mineral added is 10g / m 2 After the mixture is evenly mixed, it is applied to the surface of the slag soil; after planting, the soil is irrigated regularly by surface irrigation, ordinary sprinkler irrigation or micro-irrigation; This embodiment is recorded as group S10.

[0030] Example 2 The difference between Example 2 and Example 1 is that the moss spore powder used in Example 2 is sand moss spore powder, and the amount used is 50g / m 2 The algae culture medium used was the monophyll algae culture medium, and the addition amount was 5g / m 2 ; The amount of modified clay mineral used is 50g / m 2 ; This embodiment is recorded as group S50.

[0031] Example 3 The difference between Example 3 and Example 1 is that the moss spore powder used in Example 3 is sand moss spore powder, and the amount used is 100g / m 2 The algae culture medium used was Nostoc culture medium, and the addition amount was 10g / m 2 ; The amount of modified clay mineral used is 100g / m 2 ; This embodiment is recorded as group S100.

[0032] Example 4 The difference between Example 4 and Example 1 is that the moss spore powder used in Example 4 is spore powder of moss spores, and the amount used is 10 g / m 2 The algae culture medium used was Nostoc culture medium, and the addition amount was 1g / m 2 ; The amount of modified clay mineral used is 10g / m 2 ; This example is recorded as Group C10.

[0033] Example 5 The difference between Example 5 and Example 1 is that the moss spore powder used in Example 5 is spore powder of moss spores, and the amount used is 50g / m 2 The algae culture medium used was the culture medium of Microcoleus scaly, and the addition amount was 5g / m 2 ; The amount of modified clay mineral used is 50g / m 2 ; This example is recorded as group C50.

[0034] Example 6 The difference between Example 6 and Example 1 is that the moss spore powder used in Example 6 is spore powder of moss spores, and the amount used is 100g / m 2 The algae culture medium used was the monophyll algae culture medium, and the addition amount was 10g / m 2 ; The amount of modified clay mineral used is 100g / m 2 ; This example is recorded as group C100.

[0035] Example 7 The difference between Example 7 and Example 1 is that the moss spore powder used in Example 7 is spore powder of Mossus grosvenoris, and the amount used is 10 g / m 2 The algae culture medium used was the monophyll algae culture medium, and the addition amount was 1g / m 2 ; The amount of modified clay mineral used is 10g / m 2 ; This example is recorded as group H10.

[0036] Example 8 The difference between Example 8 and Example 1 is that the moss spore powder used in Example 8 is spore powder of Mossus grosvenoris, and the amount used is 50 g / m 2 The algae culture medium used was Nostoc culture medium, and the addition amount was 5g / m 2 ; The amount of modified clay mineral used is 50g / m 2 ; This embodiment is recorded as group H50.

[0037] Example 9 The difference between Example 9 and Example 1 is that the moss spore powder used in Example 9 is spore powder of Mossus grosvenoris, and the amount used is 100 g / m 2 The algae culture medium used was the culture medium of Microcoleus scaly, and the addition amount was 10g / m 2 ; The amount of modified clay mineral used is 100g / m 2 ; This embodiment is recorded as group H100.

[0038] Comparative Example Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that in Comparative Example 1, no algae, moss or modified clay mineral is added.

[0039] This comparative example is recorded as CK group.

[0040] Performance testing The following properties of each group of soil in Examples 1-9 and Comparative Example 1 were measured on the 30th day of daily maintenance.

[0041] 1. Ryegrass germination rate The germination rates of ryegrass under the planting methods of Examples 1-9 and Comparative Example 1 were statistically analyzed, and the results are as follows: Figure 2 shown.

[0042] Depend on Figure 2 The results showed that the germination rates of all groups except C50 and H10 were significantly different from those of CK group (P<0.05). The highest germination rate was 35.6% in C100 group, which was 34.8% higher than that of CK group. The average germination rate of sand moss group was the highest, and there was no significant difference in germination rate between S50, S100 and C100 groups (P>0.05).

[0043] 2. Soil pH The soil pH under the planting methods of Examples 1-9 and Comparative Example 1 was tested using a potentiometric method. The results are as follows: Figure 3 shown.

[0044] Depend on Figure 3 It can be seen from the results that compared with the pH value of 8.33 in the control group 1, the pH value of the embodiment group was slightly lower (P<0.05). Among them, the pH difference between the sand moss groups (S10, S50, S100) was not significant (P>0.05), and there was almost no change under different spore dosages; while the pH difference in the clumping true moss group was significant (P<0.05). When the spore dosage increased, the soil pH also decreased further, with the lowest being 8.15; the large gray moss also showed this trend, but the overall change was small.

[0045] 3. Nutrient content in soil The nutrient content of the soil under the planting methods of Examples 1-9 and Comparative Example 1 was tested. Among them, the method for determining the available nitrogen content in soil is the Kjeldahl method. The results are as follows: Figure 4 As shown; The method for determining the available potassium content in soil is to use the ammonium acetate leaching-flame photometer method. The results are as follows: Figure 5As shown; The method for determining the available phosphorus content in soil is to use the sodium bicarbonate extraction-molybdenum antimony anti-spectrophotometric method. The results are as follows: Figure 6 As shown; Depend on Figure 4 The results showed that compared with the CK group, the content of available nitrogen in all groups except S10 increased, among which the S100 group had the highest content of available nitrogen, reaching 13.41 mg / kg, an increase of 44.89% compared with the CK group; followed by the H50 group and the C100 group, with increases of 37.71% and 35.52% respectively; S100, C100, H10, and H50 all had extremely significant differences compared with the CK group (P<0.001), and all the G. gigantea groups had significant differences (P<0.05).

[0046] Depend on Figure 5 The results show that the available potassium content of all groups is higher than that of the CK group, and there are significant differences except for the H100 group (P<0.05); the available potassium content of the sand moss group increases with the increase of spore usage, among which S50 and S100 have extremely significant differences (P<0.001), and the S100 group has the highest content, which is 37.61 mg / kg, 29.33% higher than the control group; the available potassium content of the clustered true moss group is not much different at different spore dosages (P>0.05), but there are extremely significant differences compared with the control group (P<0.001), with an average increase rate of 19%; the overall available potassium content of the large gray moss group is the lowest, and there is no significant difference in potassium content among the three groups (P>0.05).

[0047] Depend on Figure 6 It can be seen from the results that there is no significant difference in the content of available phosphorus in each group (P>0.05), which may be due to the fact that the influence of several planted plants on the content of available phosphorus in the soil under the planting model provided in this application is relatively small.

[0048] 4. Organic matter content in soil The organic matter content in the soil under the planting methods of Examples 1-9 and Comparative Example 1 was tested, and the results were as follows: Figure 7 shown.

[0049] Depend on Figure 7 The results show that compared with the CK group, the organic matter content of the S10 group was almost the same, while the other groups all increased to varying degrees. Sand moss, clump-like true moss, and large gray moss all showed a trend of increasing soil organic matter content with the increase in spore dosage. The organic matter content of the large gray moss group was significantly different from that of the CK group (P<0.001), from 3.23% to 3.47% and then to 3.92%, among which the organic matter content of the H100 group increased by 100.7%.

[0050] 5. Soil enzyme activity The activities of catalase, alkaline phosphatase and sucrase in the soil under the planting methods of Examples 1-9 and Comparative Example 1 were detected using the enzyme activity kit of Suzhou Keming Biotechnology Co., Ltd.; Among them, catalase activity Figure 8 As shown; Alkaline phosphatase activity Fig. 9 As shown; Sucrase activity Fig.10 shown.

[0051] Depend on Figure 8 The results showed that compared with the CK group, the catalase content of all technical invention groups increased to varying degrees, except for H10 and H50, which showed significant differences (P<0.05); the highest S-CAT enzyme activity was 27.20 μmol / d / g in the S100 group, while that in the CK group was 17.85 μmol / d / g, an increase of 52.4% year-on-year; the comprehensive catalase activity was the highest in the sand moss group and the lowest in the large gray moss group, and the difference in enzyme activity between the two groups was quite significant at the same concentration (P<0.05).

[0052] Depend on Fig. 9 The results show that the alkaline phosphatase activity of the CK group reached 8.65 μmol / d / g, which was higher than all the example groups and had a very significant difference (P<0.001); among the example groups, the S10 group had the highest alkaline phosphatase activity of 5.74 μmol / d / g, which was 33.6% less than that of the CK group; the S-AKP / ALP activity of the sand moss group decreased with the increase of the amount of moss spores, and the enzyme activity within the group was significantly different (P<0.05); the average S-AKP / ALP activity of the large gray moss group was the highest, and there was no significant difference in enzyme activity within the group (P>0.05).

[0053] Depend on Fig.10 The results show that the S-SC activity of the CK group at 4.16 mg / d / g was higher than that of the sand moss group and the clustered true moss group, and was 1.03 times higher than the lowest group S50; the H100 group with the highest S-SC activity had a significant difference compared with the CK group (P<0.05), increasing by 10.8%; the S-SC activity of the H10 group was almost the same as that of the H100 group, and the overall S-SC activity of the large gray moss group was significantly higher than that of the sand moss group and the clustered true moss group.

[0054] In summary, during the culture period, the Nostoc-Sandmoss-Ryegrass symbiotic crust provided by the present application has the largest increase in available nitrogen content and available potassium content in the soil of this group, and the germination rate of ryegrass is at the forefront, while the available phosphorus content and soil pH are not significantly different from those of other groups. Therefore, the present application prefers ryegrass as herbaceous plant seeds, and the sowing amount is 4860 seeds / m 2; Moss spore powder is sand moss spore powder, and the addition amount is 100g / m 2 The algae culture medium is a three-stage expanded Nostoc culture medium, and the addition amount is 10g / m 2 ; The amount of modified clay mineral added is 100g / m 2 .

[0055] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A method for restoring ecologically fragile areas using algae-moss-grass symbiosis, characterized in that: The following steps are involved: S1. Deeply plow the soil in the area to be treated and cover it with soil with a thickness of 4-6 cm. Water the soil by surface irrigation, ordinary sprinkler irrigation or micro-irrigation until the soil is completely moistened. S2. Sprinkle the herb seeds evenly into the soil and gently stir the shallow soil with a shovel to cover the seeds; S3. Use a sieve to evenly spread the moss spore powder on the surface of the soil; S4. The algae are subjected to three-stage expansion to obtain a three-stage expansion algae culture solution; the three-stage expansion algae culture solution and the modified clay mineral are mixed evenly and applied to the surface of the slag; After planting, water the soil regularly by surface irrigation, ordinary sprinkler irrigation or micro-irrigation.

2. A method for restoring ecologically fragile areas using algae-moss-grass symbiosis according to claim 1, characterized in that: The herbaceous plant seeds are ryegrass seeds; the sowing amount of the ryegrass seeds is 4860 seeds / m 2 .

3. The method for restoring ecologically fragile areas by using algae-moss-grass symbiosis according to claim 1, characterized in that: The algae is one of Microcoleus spp., Nostoc spp., and Monochamus spp.

4. The method for restoring ecologically fragile areas by using algae-moss-grass symbiosis according to claim 1, characterized in that: The amount of algae culture solution added is 1-10 g / m 2 The modified clay mineral is added in an amount of 10-100 g / m 2 .

5. A method for restoring ecologically fragile areas using algae-moss-grass symbiosis according to claim 1 or 4, characterized in that: The modified clay mineral is prepared from the following raw materials in parts by weight: 10-20 parts of water, 0.2-0.4 parts of hexadecyltrimethylammonium chloride, and 0.8-1.6 parts of clay mineral.

6. A method for restoring ecologically fragile areas using algae-moss-grass symbiosis according to claim 5, characterized in that: The clay mineral is a mixture of kaolin and montmorillonite, with a mass ratio of 1:0.5-1.

5.

7. The method of restoring ecologically fragile areas by using algae-moss-grass symbiosis according to claim 5, characterized in that: The preparation method of the modified clay mineral comprises the following steps: After mixing hexadecyltrimethylammonium chloride and water, stir until dissolved, heat to 60-70° C., add clay mineral, stir and mix to react for 1-3 hours, filter, and vacuum dry to obtain modified clay mineral.

8. The method for restoring ecologically fragile areas by using algae-moss-grass symbiosis according to claim 1, characterized in that: The moss spore powder is one of sand moss spore powder, large gray moss spore powder, and cluster true moss spore powder; the moss spore powder is added in an amount of 10-100g / m 2 .

9. The method for restoring ecologically fragile areas by using algae-moss-grass symbiosis according to claim 1, characterized in that: The method for three-stage expansion of algae culture solution comprises the following steps: S1. Add the algae to the sterilized culture medium for primary expansion, mix well and place in a light constant temperature incubator, culture for 15 days at 3000LUX, 25℃, and 12h light-dark cycle, and shake once a day to prevent hardening or wall adhesion; S2. Add the mixed algae liquid obtained from the primary expansion to the sterilized culture medium for secondary expansion, place in a light constant temperature incubator, culture for 15 days at 3000LUX, 25℃, 12h light-dark cycle, and shake gently every day; S3. The third-level expansion culture is carried out in an algae culture vessel. After the algae culture vessel is sterilized with ultraviolet light for 12 hours, the sterilized culture medium is added, and then the mixed algae solution obtained from the second-level expansion culture is added. The middle lamp tube and the lower pump air valve are turned on for cultivation. The ventilation volume at the bottom is 8L per cubic minute. The culture vessel is gently shaken every day, and the culture medium is replenished after 15 days, and then cultured for another 15 days.

10. The method for restoring ecologically fragile areas by using algae-moss-grass symbiosis according to claim 1, characterized in that: The herbaceous plant seeds are ryegrass, and the sowing amount is 4860 seeds / m 2 The moss spore powder is sand moss spore powder, and the addition amount is 100g / m 2 The algae culture solution is a three-stage expanded Nostoc culture solution, and the addition amount is 10g / m 2 The modified clay mineral addition amount is 100g / m 2 .

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