Barnacle type coral regenerated gel and preparation method thereof

Abstract

The invention relates to barnacle type coral regeneration gel and a preparation method thereof, and belongs to the technical field of marine biotechnology and biological bonding, and the barnacle type coral regeneration gel is designed for repairing a coral reef ecological system so as to promote coral regeneration. The gel prepared by the method not only can ensure safe and stable reproduction of coral larvae and zooxanthellae in a marine environment, but also can provide required nutrition for growth of coral.

Description

Technical Field

[0001] The invention belongs to the field of marine biotechnology and biological bonding technology, and in particular relates to a barnacle-type coral regeneration gel and a preparation method thereof. Background Art

[0002] Due to environmental pollution, ocean warming and human activities, the coverage of coral reefs around the world is declining at an alarming rate. Faced with this grim situation, the global attention and attention to the restoration of coral reef ecosystems is increasing. Abroad, scientists are working to protect the species diversity of corals by putting coral larvae into cryopreservation banks. In my country, coral reef restoration technology has made some progress. On October 1, 2022, my country officially implemented the "Technical Guidelines for Marine Ecological Restoration Part 2: Coral Reef Ecological Restoration" (GB / T 41339.2-2022), which is my country's first technical standard for the restoration of marine ecosystems. Its specific technical means include: constructing artificial reefs, transplanting healthy corals in damaged coral reef areas, and using steel and concrete to build stable coral reef structures. These physical restoration methods enable coral reefs to basically restore their normal growth rate and health after 4 years, but the economic cost is too high.

[0003] At present, no effective biological means have been applied to the restoration of coral reef ecosystems. If biological means are used, artificially enhanced zooxanthellae and coral larvae can be fixed with gel using immobilization technology, so that the prepared gel can adhere to the coral skeleton like a barnacle, providing a safe and stable development environment for corals and zooxanthellae, improving the symbiotic interaction between coral larvae and their symbiotic zooxanthellae, increasing the coral's resistance to thermal bleaching, and accelerating the remodeling of the calcareous skeleton, thereby effectively promoting the regeneration of corals. This innovative method can provide new hope for the protection and restoration of coral reefs.

[0004] The present invention prepares a barnacle-like gel material with strong biocompatibility with coral larvae and zooxanthellae. This material uses coral skeletons as the substrate for the survival of coral larvae and zooxanthellae, enables coral larvae to reproduce safely and stably in the ocean with the help of zooxanthellae, promotes the formation of their calcareous skeletons, reshapes the coral structure at a high speed and high efficiency, and further promotes the regeneration process of corals. Summary of the invention

[0005] In view of the defects of the prior art, the purpose of the present invention is to provide a new type of barnacle-like coral regeneration gel and its preparation method, which is designed for repairing the coral reef ecosystem to promote coral regeneration. The gel prepared by this method can adhere to the coral skeleton like a barnacle and does not cause pollution to the environment. It can not only ensure the safe and stable reproduction of coral larvae and zooxanthellae in the marine environment, but also provide the necessary nutrition for the growth of coral polyps.

[0006] This gel carrier has several significant advantages: first, the choice of substrate can provide coral polyps with calcium ions necessary for partial development, promote the development of coral larvae, and on the other hand, serve as an effective support structure to provide them with a safe and stable growth space. Secondly, the barnacle-like gel can increase the attachment rate and survival rate of coral larvae, and strengthen the symbiotic relationship with zooxanthellae, providing a solid foundation for the natural restoration of coral reefs. In addition, the environmental friendliness and degradability of the gel material reduce secondary pollution to the marine environment and ensure the sustainability of the restoration work. Compared with traditional physical restoration methods, the biological restoration method of the present invention has lower economic costs and higher ecological benefits, providing an economical and efficient solution for the long-term protection and restoration of coral reefs.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is:

[0008] A barnacle-like coral regeneration gel, characterized in that it is synthesized from gel beads for initially fixing coral larvae and zooxanthellae, coral skeletons, biological adhesives and ethylene glycol diglycidyl ether (EGDE). Among them:

[0009] The gel beads for initially fixing coral larvae and zooxanthellae have the following components and the weight ratio of each component is as follows:

[0010] 1-5 parts of Cladocopium goreaui,

[0011] 1-5 copies of coral larvae Acropora robusta,

[0012] 1 to 3 parts of 1 to 2% propylene glycol alginate solution,

[0013] Calcium chloride solution.

[0014] Bioadhesives contain:

[0015] 1-5% 3,4-dihydroxyphenylalanine solution and 1-5% tyrosinase solution.

[0016] A barnacle-like coral regeneration gel has the following components and the weight ratio of each component is as follows:

[0017]

[0018] The preparation method of the barnacle-like coral regeneration gel comprises the following steps: 1) taking the culture solution of coral larvae and zooxanthellae, mixing it with propylene glycol alginate (PGA) solution, and dripping the mixed solution into a calcium chloride solution to initially cross-link and form gel beads; 2) placing the gel beads soaked in 3,4-dihydroxyphenylalanine (DOPA) solution on the surface of coral bones coated with DOPA, ensuring that the gel beads and the surface of the coral bones are in close contact, so that DOPA forms a thin adhesive layer between the two surfaces; 3) placing the coral bones to which the gel beads have been adhered into a tyrosinase solution to solidify DOPA; and 4) subsequently placing an ethylene glycol diglycidyl ether (EGDE) solution to obtain the barnacle-like coral regeneration gel.

[0019] Furthermore, the preparation method of the barnacle-like coral regeneration gel comprises the following steps:

[0020] 1. Preparation of Gel Beads for Initial Immobilization of Coral Larvae and Zooxanthellae

[0021] (1) Prepare a culture medium containing larvae of Acropora robusta and zooxanthellae (Cladocopium goreaui).

[0022] Add 2-10 parts of filtered natural seawater to L1 medium, add 0.5-1.5 parts of glucose, stir for 2 minutes, and then slowly add 1-5 parts of Acropora robusta and Cladocopium goreaui in an environment of 16.72-28.40℃. Use a photoperiod of 12 hours of light and 12 hours of darkness to simulate natural conditions, with a light intensity of 90μE / m 2 / s. Keep the experimental environment ventilated and culture for 1 to 2 days.

[0023] (2) Preparation of propylene glycol alginate (PGA) solution:

[0024] A. Esterification reaction

[0025] Add 0.5-1 part of sodium alginate (SA) to the reaction bottle, mix it with filtered natural seawater, place it in a 40°C constant temperature water bath shaker and heat it until it dissolves to form a 0.5%-1.0% SA solution, and cool it to room temperature.

[0026] Add 3 to 6 parts of filtered natural seawater, 0.5 to 0.9 parts of SA solution, 1.5 to 2.7 parts of propylene oxide, and finally 0.1 to 0.3 parts of sodium hydroxide to the pressure reactor in sequence, react for 3 to 5 hours at a working pressure of 10 to 20 bar and 55 to 80° C., and cool to room temperature.

[0027] B. Neutralization, extraction and purification

[0028] After the esterification reaction, the product needs to be neutralized to prevent overreaction or side reactions. Add an appropriate amount of acetic acid to the reactor and use a pH meter to monitor the pH of the solution and adjust it to about 6.5 to 7.5.

[0029] Add 3-10 parts of 95% ethanol solution to the reactor, place it on a water bath shaker and mix at 120 rpm for 20 minutes to precipitate PGA. Filter the product, dissolve the filtered precipitate in a small amount of water, and cool it at -10°C for 30 minutes to crystallize PGA. Perform a secondary filtration to further remove impurities.

[0030] C. Washing and drying:

[0031] Take a reaction bottle, add appropriate amount of water, add PGA precipitate obtained in step B, add 4-10 parts of methanol, stir for 5 minutes, filter to remove unreacted raw materials and by-products. Repeat this process 3-5 times.

[0032] The washed PGA was dried in a vacuum oven for 2 h to remove the residual solvent and moisture. After drying, the PGA formed a powdery solid.

[0033] D. Preparation of PGA Solution

[0034] PGA is dissolved in water to form a PGA solution with a concentration of 1 to 2%.

[0035] (3) Preliminary cross-linking:

[0036] Take a reaction bottle, add 1.5-4 parts of calcium chloride, an appropriate amount of filtered natural seawater, and stir to form a 2-5% calcium chloride solution. Take another reaction bottle, take 1-3 parts of culture medium containing Acropora robusta coral larvae and zooxanthellae (Cladocopium goreaui), and mix with 1-3 parts of 1-2% PGA solution. Use a pipette to drop the mixed solution into the calcium chloride solution, initially cross-link to form gel beads, and let it stand for 30-60 minutes.

[0037] (4) Cleaning gel beads:

[0038] The gel beads were removed and gently washed with deionized water to remove excess calcium chloride on the surface.

[0039] 2. Preparation and adhesion of bioadhesive

[0040] (1) Preparation of 3,4-dihydroxyphenylalanine (DOPA) solution

[0041] A. Synthesis of 3-bromo-L-tyrosine

[0042] Take a reaction bottle, add 5-9 parts of hydrogen bromide (HBr) and an appropriate amount of water, stir until the HBr is dissolved, and prepare a 48% hydrogen bromide aqueous solution. Add 2-5 parts of L-tyrosine to the hydrogen bromide aqueous solution, place it in a 0-5°C constant temperature water bath shaker and mix at 120 rpm for 2 hours.

[0043] Then, the reaction bottle was taken out, the solution was warmed to room temperature, and placed in a 30°C constant temperature water bath shaker and mixed at 120 rpm for 30 min.

[0044] 300 ml of cold water at 10°C was taken, and the reaction solution and the cold water were quickly mixed to precipitate a solid, which was filtered and then washed with cold water to obtain 3-bromo-L-tyrosine.

[0045] B. Hydroxylation reaction

[0046] Take 1-3 parts of 3-bromo-L-tyrosine prepared in step A, dissolve in 100 ml filtered natural seawater, add 0.2-0.5 parts of sodium hydroxide, 0.05-0.1 parts of copper iodide (CuI), place in a 25°C constant temperature water bath shaker and mix at 120 rpm for 3 hours. Filter, and add acetic acid to neutralize the filtrate to pH 6.5-7.5.

[0047] Add 95% ethanol solution to the reaction solution to precipitate DOPA, filter it, and dry it in a vacuum drying oven for 2 hours to obtain purified DOPA.

[0048] The purified DOPA is dissolved in an appropriate amount of water to prepare a DOPA solution with a concentration of 1 to 5%.

[0049] (2) Soaking and coating:

[0050] At room temperature, take a 1-5% DOPA solution, soak 2-6 washed gel beads in the solution, remove them after 5 minutes, and use a sterilized wool brush to apply the DOPA solution on the surface of 5-10 cm coral bones. Make sure that the surface of the gel beads and coral bones are evenly covered with DOPA solution.

[0051] (3) Adhesion:

[0052] At room temperature, gently place the soaked gel beads on the surface of the coral bone coated with DOPA solution, gently press the gel beads, and maintain the pressure for about 1 to 2 minutes to ensure close contact between the gel beads and the coral bone surface, allowing DOPA to form a thin adhesive layer between the two surfaces.

[0053] (4) Solidification of DOPA:

[0054] Take a reaction bottle, add 0.3-0.8 parts of tyrosinase and filtered natural seawater to form a 1-5% tyrosinase solution. Put the coral bones with gel beads attached into the solution to oxidize DOPA and accelerate the curing process of DOPA adhesive. Take it out after standing for 1-3 hours.

[0055] 3. Synthesis of Immobilized Gel

[0056] Add 0.8-1 part of ethylene glycol diglycidyl ether (EGDE) to the reaction bottle, mix with filtered natural seawater, place in a 40°C constant temperature water bath shaker and mix at 120 rpm for 20 minutes to form an EGDE solution with a concentration of 0.8%-1.0%, and cool to room temperature. Put the coral bones with gel beads attached into the solution and let it stand for 30 minutes, and the barnacle-like coral regeneration gel is ready.

[0057] Compared with the prior art, the present invention has the following beneficial effects:

[0058] The present invention proposes an innovative barnacle-like coral regeneration gel, which uses biotechnology to repair coral reef ecosystems through optimized bioadhesives and cross-linking technology. Compared with traditional physical repair methods, the bioremediation method of the present invention has lower economic costs and higher ecological benefits, providing an economical and efficient solution for the long-term protection and restoration of coral reefs.

[0059] First, the present invention prepares gel beads with propylene glycol alginate (PGA) and calcium chloride (CaCl2) as the main body, embeds coral larvae and zooxanthellae, has strong biocompatibility, ensures that coral larvae and zooxanthellae can grow normally, promotes the establishment of their symbiotic relationship, and significantly improves the survival rate and growth rate of coral larvae and zooxanthellae. In this process, Ca in calcium chloride 2+ It undergoes cross-linking reactions with the carboxyl (-COOH) and hydroxyl (-OH) groups in PGA to form gel beads that entrap coral larvae and zooxanthellae.

[0060] At the same time, the 3,4-dihydroxyphenylalanine (DOPA) solution prepared by the present invention and the adhesion method catalyzed by tyrosinase enable the gel beads to firmly adhere to the surface of the coral bones to form a stable adhesion layer, providing a stable and safe growth and development space for corals and zooxanthellae. In this process, the hydroxyl (-OH) and carboxyl (-COOH) in DOPA can undergo esterification reaction and hydrogen bonding with the hydroxyl (-OH) and carboxyl (-COOH) in PGA to form a stable cross-linked structure. In addition, DOPA undergoes coordination reaction with calcium ions on the coral bones to further stabilize the gel structure. Under the catalysis of tyrosinase, part of DOPA is oxidized to generate dopaquinone, which can undergo Michael addition reaction with unoxidized DOPA to form a cross-linked polydopamine (PDA) structure, providing a stable adhesion layer, so that the gel beads can firmly adhere to the surface of the coral bones.

[0061] In addition, ethylene glycol diglycidyl ether (EGDE) was added, and EGDE underwent a cross-linking reaction with unreacted PGA: the epoxy groups in ethylene glycol diglycidyl ether (EGDE) underwent a ring-opening reaction with the hydroxyl groups (-OH) in PGA to form ether bonds, which further enhanced the mechanical strength and stability of the gel beads, allowing the prepared carrier to remain stable in a seawater environment.

[0062] The preparation method of the present invention has clear steps, is easy to operate, and can be implemented under conventional laboratory conditions. In addition, although the present invention only uses Acropora robusta larvae and Cladocopium goreaui, the method is applicable to the cultivation and regeneration of various corals and zooxanthellae, and has wide applicability.

[0063] According to the test, the barnacle-like coral regeneration gel prepared by the present invention has a strong mechanical strength of 73 to 89 mN, and can achieve the immobilization of coral larvae and zooxanthellae in a seawater environment, a temperature of 16.2 to 37.4°C, and a variable environment of PH6.7 to PH9.1, with an efficiency of 80.87% to 95.72%. The bioadhesive can firmly adhere to the gel beads and will not fall off in the seawater waves. In addition, the carrier has good biocompatibility, and the immobilized coral larvae and zooxanthellae can show good biological activity, and the relative biological activity is about 65% higher than that of the free state. BRIEF DESCRIPTION OF THE DRAWINGS

[0064] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0065] Figure 1 is a flow chart of the preparation method of the present invention;

[0066] Figure 2 is a gel image of gel beads for preliminarily fixing coral larvae and zooxanthellae in Example 1 of the present invention;

[0067] Figure 3 This is a schematic diagram of the principle of preparing PGA in Example 1 of the present invention;

[0068] Figure 4 It is a schematic diagram of the principle of preparing DOPA in Example 1 of the present invention. DETAILED DESCRIPTION

[0069] The technical solution of the present invention is further specifically described below in conjunction with examples.

[0070] Example 1

[0071] like Figure 1 As shown, a method for preparing a barnacle-like coral regeneration gel in this embodiment comprises the following steps:

[0072] 1. Preparation of Gel Beads for Initial Immobilization of Coral Larvae and Zooxanthellae

[0073] (1) Prepare a culture medium containing larvae of Acropora robusta and zooxanthellae (Cladocopium goreaui).

[0074] 300 ml of filtered natural seawater was added to L1 medium, 8 g of glucose was added, stirred for 2 min, and then Acropora robusta and Cladocopium goreaui were slowly added at 26 °C. A photoperiod of 12 h light and 12 h dark was used to simulate natural conditions, with a light intensity of 90 μE / m 2 / s. Keep the experimental environment ventilated and culture for 1 day.

[0075] (2) Preparation of propylene glycol alginate (PGA) solution:

[0076] A. Esterification reaction

[0077] 2.8 g of sodium alginate (SA) was added to the reaction bottle, mixed with filtered natural seawater, placed in a 40° C. constant temperature water bath shaker and heated until it dissolved to form a 0.8% SA solution, and cooled to room temperature.

[0078] 100 ml of filtered natural seawater, 200 ml of 0.8% SA solution, 13 g of propylene oxide and finally 1 g of sodium hydroxide were added to the pressure reactor in sequence. The reaction was carried out at a working pressure of 10 bar and 70° C. for 3.5 hours and then cooled to room temperature.

[0079] B. Neutralization, extraction and purification

[0080] After the esterification reaction, the product needs to be neutralized to prevent overreaction or side reactions. Add an appropriate amount of acetic acid to the reactor and use a pH meter to monitor the solution pH and adjust it to 7.

[0081] Add 500 ml of 95% ethanol solution to the reactor, place it on a water bath shaker and mix at 120 rpm for 20 minutes to precipitate PGA. Filter the product, dissolve the filtered precipitate in a small amount of water, and cool it at -10°C for 30 minutes to crystallize PGA. Perform a secondary filtration to further remove impurities.

[0082] C. Washing and drying:

[0083] Take a reaction bottle, add 200 ml of water, add the PGA precipitate obtained in step B, add 30 g of methanol, stir for 5 min, and filter to remove unreacted raw materials and by-products. Repeat this process 4 times.

[0084] The washed PGA was dried in a vacuum oven for 2 h to remove the residual solvent and moisture. After drying, the PGA formed a powdery solid.

[0085] D. Preparation of PGA Solution

[0086] 1.5 g of PGA powder was dissolved in water to form a PGA solution with a concentration of 1.5%.

[0087] (3) Preliminary cross-linking:

[0088] Take a reaction bottle, add 12g calcium chloride and 300ml filtered natural seawater, stir to form a 4% calcium chloride solution. Take another reaction bottle, take 50ml of culture medium containing Acropora robusta coral larvae and zooxanthellae Cladocopium goreaui, and mix with 50ml of 1.5% PGA solution. Use a pipette to add the mixed solution dropwise to the calcium chloride solution, initially cross-link to form gel beads, and let stand for 40 minutes.

[0089] (4) Cleaning gel beads:

[0090] The gel beads were removed and gently washed with deionized water to remove excess calcium chloride on the surface.

[0091] 2. Preparation and adhesion of bioadhesive

[0092] (1) Preparation of 3,4-dihydroxyphenylalanine (DOPA) solution

[0093] A. Synthesis of 3-bromo-L-tyrosine

[0094] Take a reaction bottle, add 48g hydrogen bromide (HBr) and 100ml water, stir until HBr is dissolved, and obtain a 48% hydrogen bromide aqueous solution. Add 18g L-tyrosine to the hydrogen bromide aqueous solution, place in a 3°C constant temperature water bath shaker and mix at 120rpm for 2h.

[0095] Then, the reaction bottle was taken out, the solution was warmed to room temperature, and placed in a 30°C constant temperature water bath shaker and mixed at 120 rpm for 30 min.

[0096] 300 ml of cold water at 10°C was taken, and the reaction solution and the cold water were quickly mixed to precipitate a solid, which was filtered and then washed with cold water to obtain 3-bromo-L-tyrosine.

[0097] B. Hydroxylation reaction

[0098] Take 10g of 3-bromo-L-tyrosine prepared in step A, dissolve it in 100ml filtered natural seawater, add 2g of sodium hydroxide and 0.5g of copper iodide (CuI), place it in a 25℃ constant temperature water bath shaker and mix it at 120rpm for 3h. Filter it, and add acetic acid to neutralize the filtrate to pH=7.

[0099] Add 400 ml of 95% ethanol solution to the reaction solution to precipitate DOPA, filter it, and dry it in a vacuum drying oven for 2 hours to obtain purified DOPA.

[0100] The purified DOPA was dissolved in an appropriate amount of water to prepare a 5% DOPA solution.

[0101] (2) Soaking and coating:

[0102] At room temperature, take 30 ml of 5% DOPA solution, soak 15 g of washed gel beads in the solution, remove them after 5 minutes, and use a sterilized wool brush to apply the DOPA solution on the surface of 5-10 cm coral bones. Make sure that the surface of the gel beads and coral bones are evenly covered with DOPA solution.

[0103] (3) Adhesion:

[0104] At room temperature, gently place the soaked gel beads on the surface of the coral bone coated with DOPA solution, gently press the gel beads, and maintain the pressure for about 1 to 2 minutes to ensure close contact between the gel beads and the coral bone surface, allowing DOPA to form a thin adhesive layer between the two surfaces.

[0105] (4) Solidification of DOPA:

[0106] Take a reaction bottle, add 3g of tyrosinase and filtered natural seawater to form a 1% tyrosinase solution. Put the coral bones with gel beads attached into the solution to oxidize DOPA and accelerate the curing process of DOPA adhesive. Take it out after standing for 1 hour.

[0107] 3. Synthesis of Immobilized Gel

[0108] Add 0.9g of ethylene glycol diglycidyl ether (EGDE) to the reaction bottle, mix with 100ml of filtered natural seawater, place in a 40℃ constant temperature water bath shaker and mix at 120rpm for 20min to form a 0.9% EGDE solution, and cool to room temperature. Place the coral bones with gel beads attached into the solution and let stand for 30min, and the barnacle-like coral regeneration gel is ready.

[0109] 4. Testing the physical and chemical properties of coral regeneration gel

[0110] The physical and chemical properties of the synthesized coral regeneration gel, such as adsorption capacity, specific surface area, mechanical strength, and immobilization efficiency, were tested. The test results are shown in Table 1.

[0111] Table 1 Physical and chemical properties test table of immobilized carrier

[0112]

[0113] According to the test, the barnacle-like coral regeneration gel prepared by the present invention has a strong mechanical strength of 73 to 89 mN, and can achieve the immobilization of coral larvae and zooxanthellae in a seawater environment, a temperature of 16.2 to 37.4°C, and a variable environment of PH6.7 to PH9.1, with an efficiency of 80.87% to 95.72%. The bioadhesive can firmly adhere to the gel beads and will not fall off in the seawater waves. In addition, the carrier has good biocompatibility, and the immobilized coral larvae and zooxanthellae can show good biological activity, and the relative biological activity is about 65% higher than that of the free state.

[0114] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A barnacle-like coral regeneration gel, characterized in that: The coral regeneration gel is synthesized from gel beads for preliminarily fixing coral larvae and zooxanthellae, coral bones, biological adhesive and ethylene glycol diglycidyl ether; the biological adhesive includes 3,4-dihydroxyphenylalanine solution and tyrosinase solution; The gel beads are placed on the surface of coral bones coated with 3,4-dihydroxyphenylalanine solution, the coral bones with gel beads attached are placed in a tyrosinase solution to solidify 3,4-dihydroxyphenylalanine, and then placed in an ethylene glycol diglycidyl ether solution to obtain a barnacle-like coral regeneration gel.

2. The coral regeneration gel according to claim 1, characterized in that: The gel beads for preliminarily fixing the coral larvae and zooxanthellae are gel beads mainly composed of propylene glycol alginate and calcium chloride, and embed the coral larvae and zooxanthellae.

3. The coral regeneration gel according to claim 1, characterized in that: The bioadhesive comprises a 3,4-dihydroxyphenylalanine solution with a concentration of 1 to 5% and a tyrosinase solution with a concentration of 1 to 5%.

4. The coral regeneration gel according to claim 1, characterized in that: 2 to 6 parts of gel beads for preliminarily fixing coral larvae and zooxanthellae, 1 to 3 parts of coral bones, 0.8 to 1.5 parts of biological adhesive, and 0.8 to 1 part of ethylene glycol diglycidyl ether.

5. A method for preparing a barnacle-like coral regeneration gel according to any one of claims 1 to 4, comprising placing the gel beads on the surface of a coral bone coated with a 3,4-dihydroxyphenylalanine solution, placing the coral bone to which the gel beads have been adhered into a tyrosinase solution, solidifying the 3,4-dihydroxyphenylalanine, and then placing the coral bone into an ethylene glycol diglycidyl ether solution, thereby preparing a barnacle-like coral regeneration gel.

6. The method for preparing a barnacle-like coral regeneration gel according to claim 5, characterized in that: The preparation method comprises the following steps: 1) taking the culture solution of coral larvae and zooxanthellae, mixing it with propylene glycol alginate solution, and dripping the mixed solution into calcium chloride solution for preliminary cross-linking to form gel beads; 2) placing the gel beads on the surface of coral bones coated with 3,4-dihydroxyphenylalanine solution to ensure close contact between the gel beads and the surface of the coral bones; 3) placing the coral bones to which the gel beads have been adhered into a tyrosinase solution to solidify the 3,4-dihydroxyphenylalanine; and 4) subsequently placing an ethylene glycol diglycidyl ether solution to obtain a barnacle-like coral regeneration gel.

7. The method for preparing a barnacle-like coral regeneration gel according to claim 5, characterized in that: The concentration of the propylene glycol alginate solution is 1-2%.

8. The method for preparing a barnacle-like coral regeneration gel according to claim 5, characterized in that: The concentration of the ethylene glycol diglycidyl ether solution is 0.8% to 1.0%.

9. Use of a barnacle-like coral regeneration gel according to any one of claims 1 to 4, wherein the coral regeneration gel is used to promote coral regeneration.

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