Biological treatment method and application of sorghum vinasse

Abstract

The invention relates to the technical field of biological fermentation, in particular to a biological treatment method for sorghum vinasse and application thereof.The biological treatment method comprises the steps that sorghum vinasse, corn flour, soybean meal, wheat bran, ammonium sulfate, monopotassium phosphate, calcium carbonate and sodium bicarbonate are evenly mixed according to the material-water ratio of 1: 1.2, autoclaved sterilization and cooling are conducted, 0.8% of an enzyme preparation is sequentially added, fermentation is conducted for one day at the temperature of 30 DEG C, and then fermentation is conducted for one day at the temperature of 30 DEG C; and adding 9.6% of a complex microbial inoculant again, fermenting at 30 DEG C for 4 days, finally adding 0.06% of lactic acid bacteria, fermenting for 1 day, drying at 45 DEG C, and crushing. The water content in the treated sorghum vinasse is reduced by 6%, the crude protein content is increased by 5%, the neutral detergent fiber content is reduced by 10%, and the acid detergent fiber content is reduced by 8%. The sorghum vinasse prepared by the method disclosed by the invention is used as a protein raw material of the meat duck compound feed, and is added into the meat duck compound feed according to the weight of 10-25%, so that an ideal weight increasing effect can be achieved, the breeding cost is remarkably reduced, and the method is simple to operate and obvious in effect.

Description

Technical Field

[0001] The invention relates to the technical field of biological fermentation, in particular to a biological treatment method for sorghum distiller's grains and an application thereof. Background Art

[0002] In the context of large-scale farming, the shortage of protein feed is more prominent. On the one hand, it is reflected in the huge demand for protein feed in the domestic feed industry and animal husbandry. In order to ensure the stable supply of protein feed, they have to rely on imports; on the other hand, it is reflected in the price of protein feed. Take soybean meal as an example. Soybean meal is the most widely used plant protein feed. Affected by the contradiction between market supply and demand, the price of soybean meal has continued to rise, which has compressed the profit space of the feed industry and animal husbandry to the greatest extent. The nutritional content of sorghum distiller's grains on a dry matter basis is as follows: dry matter 88.70%, crude protein 24.12%, starch 12.20%, crude ash 10.13%, crude fat 7.95%, crude fiber 17.12%, neutral detergent fiber 43.54%, acid detergent fiber 36.92%, acid detergent lignin 10.40%, nitrogen-free extract 29.37%, calcium 0.58%, total phosphorus 0.59%, iron 1541.93 mg / kg, copper 29.28 mg / kg, manganese 226.69 mg / kg, zinc 103.21 mg / kg. The anti-nutritional factors on a dry matter basis are as follows: tannin 137.78 mg / kg, silicon 18.61 g / kg. From the perspective of the nutrient content of feed, the crude fiber content in sorghum distiller's grains is 18%, and the crude protein content is ≥20% in the dry matter, which is included in the category of protein feed. It has the value of protein feed development and utilization to meet the needs of the development of feed industry and breeding industry.

[0003] Li Qian et al.'s research shows that the anti-nutritional factor content of sorghum distiller's grains of sauce-flavor and strong-flavor types is higher than that of corn distiller's grains, beer grains, and highland barley distiller's grains. Sorghum distiller's grains belong to the sauce-flavor type, which contains a large amount of cellulose, lignin and silica, and has poor palatability, which seriously affects the digestion and absorption of nutrients by animals and reduces the nutritional value of distiller's grains. At the same time, tannins in distiller's grains can combine with proteins to form insoluble complexes, passivate digestive enzymes such as proteases and lipases, thereby hindering the digestion and absorption of nutrients such as protein and fat; it can affect the quality of animal products and reproductive performance. At present, the most commonly used methods are microbial fermentation, silage, and enzymolysis. The microbial fermentation method is to play a role through various enzymes produced by fermentation. Although a large amount of organic acids and flavor substances will be produced during the fermentation process, it has positive significance for improving the palatability of feed and regulating the intestinal health of animals. However, the single microbial fermentation method produces insufficient enzyme activity and cannot meet actual production needs. The enzymolysis method uses enzymes to destroy the structure of anti-nutritional factors and decompose them into easily digestible and absorbable substances, but bitter substances are easily produced during the enzymolysis process, which affects the palatability of feed products.

[0004] At present, in the existing feed production process, distiller's grains have been used to a certain extent. The publication number is: CN104431535A, and the name is: A feed for promoting the growth and development of broilers and a preparation method thereof. The feed contains 12-14 parts of sorghum distiller's grains. The preparation method is that the sorghum distiller's grains are directly mixed with other raw materials, dehydrated or granulated to prepare granular materials; in this method, the sorghum distiller's grains are directly used without any treatment, and its nutritional value cannot be reflected, and the usage is limited. The publication number is: CN104431295A, and the name is: A method for preparing livestock feed using distiller's grains. In this method, the distiller's grains are first dried, and then microwaved and ammoniated in sequence, and then the treated distiller's grains are fermented with Candida utilis, Trichoderma koningii and lactic acid bacteria to prepare livestock feed. The feed prepared by this method has a high protein content and can effectively increase the weight of livestock and improve the meat quality of livestock; this method adopts a single microbial fermentation method, and the enzyme activity produced is insufficient and cannot meet actual production needs. The publication number is: CN105981972A, and the name is: A nutritious distiller's grains duck feed and a preparation method thereof, the raw materials comprising: 70-80 corn flour, 30-40 sorghum, 10-15 black rice, 5-8 brown sugar, 6-9 burdock, 4-5 dark soy sauce, 6-10 matcha powder, 10-13 fig powder, 4-5 black tea powder, 8-13 sesame oil, 70-80 distiller's grains, 1-2 patchouli, 1-2 chrysanthemum, 1-2 licorice, fiber enzyme; the method of processing the wine lees is as follows: drying the wine lees, separating the husks with an oscillating screen, and then putting them into a grinder for pounding without a screen, and placing a 20-mesh oscillating screen in the cyclone separator of the grinder, pounding and sieving to separate the husks; after the wine lees are powdered, they are mixed with corn flour and fried to obtain mixed flour; after the separated husks are crushed, appropriate amount of water and cellulase are added and mixed, the pH is adjusted to 5, the temperature is maintained at 50-60°C, and enzymolysis is performed for 45-50 hours to obtain enzymolysis materials. The publication number is: CN105901315A, and the name is: A feed for promoting the growth of meat ducklings. The raw materials include: 20-25 parts of glutinous corn flour, 12-18 parts of rice bran, 10-15 parts of sorghum distiller's grains, 8-12 parts of broad bean pods, 6-10 parts of climbing bean root powder, 5-8 parts of papaya powder, 2-5 parts of auricularia auricula, 2-4 parts of bitter melon seeds, 2-4 parts of pumpkin leaves, 4-6 parts of purple sweet potato whole powder, 2-5 parts of petitgrain leaves, 1-2 parts of insect protein, 2-4 parts of chestnut roots, 3-6 parts of Solanum esculentum, 0.5-0.8 parts of pomegranate seeds, and 0.2-0.5 parts of polygonatum extract. The sorghum distiller's grains are not subjected to any treatment and are directly crushed and used after drying. The publication number is: CN116831219A, and the name is: A biological feed prepared using distiller's grains and a preparation method thereof, which is prepared from distiller's grains, corn flour, baking soda, calcium hydrogen phosphate, lysine iron, cellulase, red yeast rice and a composite bacterial agent.The specific method is as follows: pre-treat the vinasse and mix it with cellulase for enzymolysis to obtain the enzymolyzed vinasse; mix it with red yeast rice and composite bacterial agent for sealed fermentation, then add corn flour, calcium hydrogen phosphate and lysine iron to the fermented vinasse and mix them evenly, and finally dry and crush them to obtain the biological feed. The fermentation process of this method does not ferment corn flour, and the non-starch polysaccharides contained in it are generally difficult to be decomposed by the digestive enzymes secreted by monogastric animals themselves, thereby affecting the absorption of animal nutrients. In addition, during the application process, the biological feed can only be added to traditional feed at 5-10% for feeding pigs. Summary of the invention

[0005] The purpose of the present invention is to provide a sorghum distiller's grains biological treatment method and application in view of the problem that the usage of sorghum distiller's grains is relatively low in the prior art.

[0006] To achieve the above object, the technical solution adopted by the present invention is: a biological treatment method for sorghum distiller's grains, comprising the following steps:

[0007] (1) Mix sorghum distiller's grains, corn flour, soybean meal, wheat bran, ammonium sulfate, potassium dihydrogen phosphate, calcium carbonate and sodium bicarbonate evenly at a material-water ratio of 1:1.2, sterilize at 121°C for 20 minutes and then cool;

[0008] (2) Adding a composite enzyme preparation at 0.8% of the weight of the mixture, and fermenting and culturing at 30-32°C for 1 day, wherein the composite enzyme preparation comprises cellulase H type, laccase 10000G and tannase, and the weight ratios of the three to the mixture are 0.5%, 0.15% and 0.15% respectively;

[0009] (3) After one day of fermentation, a mixed bacterial agent was inoculated at 9.6% of the weight of the material, and fermented at 30-32°C for 4 days. The composite bacterial agent was Bacillus subtilis (CICC23830), Aspergillus niger (CICC41254) and white rot fungus (ACCC30942), and the dosage ratio was 1:1:1;

[0010] (4) After 5 days of fermentation, inoculate the material with lactic acid bacteria at 0.06% of the material weight, ferment and culture at 30-32°C for 1 day, take out the material, and dry and crush it in a forced air drying oven at 45°C.

[0011] Furthermore, in step (1), the weight ratio of each raw material is: 62.0% sorghum grains, 6.2% corn flour, 6.2% soybean meal, 12.4% wheat bran, 1.9% ammonium sulfate, 0.4% potassium dihydrogen phosphate, 6.2% calcium carbonate, and 4.7% sodium bicarbonate.

[0012] Furthermore, in step (2), the enzyme activity of the cellulase H type is ≥10000 U / g, the enzyme activity of the laccase 10000G is ≥10000 U / kg, and the enzyme activity of the tannin is ≥300 U / g.

[0013] Cellulase H, laccase and tannase were purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0014] Furthermore, in step (3), the concentration of Bacillus subtilis (CICC23830) is 1.6×10 7 CFU / mL, Aspergillus niger (CICC41254) concentration was 2.2×10 7 CFU / mL, white rot fungus (ACCC30942) concentration was 5.1×10 7 CFU / mL. Purchased from China Industrial Microbiological Culture Collection Administration Center.

[0015] Furthermore, in step (4), the lactic acid bacteria is Lactobacillus acidophilus, and the concentration is 1.1×10 11 CFU / g. Lactic acid bacteria were purchased from Jiangxi Haoshiwo Biotechnology Co., Ltd. and were Lactobacillus acidophilus Woshun SS-1000.

[0016] Furthermore, in steps (2), (3) and (4), the fermentation temperature is 30°C.

[0017] Through the treatment method of the present invention, the moisture content in sorghum distiller's grains decreased from 13.76% to 7.62%, the crude protein content increased from 21.45% to 26.44%, the neutral detergent fiber decreased from 47.88% to 37.55%, and the acid detergent fiber decreased from 35.96% to 27.58%.

[0018] The sorghum distiller's grains prepared by the method of the invention are used as a protein raw material for compound feed for broiler ducks and are added to the compound feed for broiler ducks at 10-25% by weight, preferably 10%.

[0019] The beneficial technical effect of the present invention is that the moisture content in sorghum distiller's grains is reduced by 6 percentage points, the crude protein content is increased by 5 percentage points, the neutral detergent fiber is reduced by 10 percentage points, and the acid detergent fiber is reduced by 8 percentage points through the treatment of the present invention. The sorghum distiller's grains prepared by the present invention are used as a protein raw material for meat duck compound feed, and 10-25% by weight is added to the meat duck compound feed to achieve an ideal weight gain effect and significantly reduce the breeding cost. The method is simple to operate and has obvious effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0021] Figure 1 It is a response surface diagram of the interaction between fermentation temperature and fermentation time on the enzyme activity content of sorghum wine grains fermented with bacterial enzymes in the present invention.

[0022] Figure 2 It is a response surface diagram of the interaction between the inoculation amount and the fermentation time on the enzyme activity content of sorghum wine grains fermented with bacterial enzymes in the present invention.

[0023] Figure 3 It is a response surface diagram of the interaction between the inoculation amount and the fermentation temperature on the enzyme activity content of sorghum wine grains fermented with bacterial enzymes in the present invention. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] 1. Response surface optimization of sorghum distiller's grains degradation

[0026] 1.1. Response surface test factors and levels

[0027] On the basis of the single factor results, a three-factor three-level experimental design was carried out with fermentation time h (A), fermentation temperature ℃ (B), inoculation amount % (C), and enzyme activity % as influencing factors, and cellulase activity as the response value. The results are shown in Table 1.

[0028] Table 1 Factors and levels of Box-Behnken response surface experiment

[0029]

[0030] 1.2. Box-Behnken response surface design results

[0031] On the basis of the single factor results, a three-factor three-level experimental design was carried out with fermentation time h (A), fermentation temperature ℃ (B), inoculation amount % (C), and enzyme activity % as influencing factors, and cellulase activity as the response value. The results are shown in Table 2.

[0032] Table 2 Box-Behnken response surface design results

[0033]

[0034] 2. ANOVA of regression model

[0035] The regression model corresponding to the enzyme activity response value was subjected to variance analysis and a significance test. The results are shown in Table 2. The quadratic regression model of enzyme activity: F=28.28, P<0.0001 shows that this regression model is extremely significant; the lack of fit test F=0.77, P=0.5685>0.05, the lack of fit is not significant, indicating that the model is acceptable. The R2 of the regression model is greater than 0.9, indicating that the model fits the experiment well and has a high degree of credibility. The model can explain most of the changes in the response value, and the three regression equations can be used to predict the enzyme activity. The quadratic regression equation is as follows:

[0036] Enzyme activity = 6.43-0.029A+0.17B+0.13C+0.19AB+0.25AC+0.31BC-1.13A 2 -0.92B 2 -0.52C 2

[0037] Table 3 Analysis of variance of regression model

[0038]

[0039] 3. Test of regression coefficients of different experimental factors

[0040] The F value and P value in the regression model reflect the significance of each factor in the model. The larger the F value and the smaller the P value, the more significant the factor is in the model. As can be seen from Table 3, fermentation time and fermentation temperature have a significant effect on the cellulase activity content of sorghum distiller's grains fermented with bacterial enzymes (P<0.01). The influence of the three factors on it is ranked as fermentation temperature>fermentation time>inoculation amount.

[0041] Table 4 Test of regression coefficients of different experimental factors

[0042]

[0043] 4. Response Surface Interaction Analysis

[0044] The three-dimensional surface diagram of the response surface shows the results of each response value under the interaction of different experimental factors, which can intuitively reflect the degree of influence of each experimental factor on the response surface. The greater the interaction between the two factors, the steeper the response surface, the denser the contour lines, and the three-dimensional diagram will be elliptical; the smaller the interaction between the two factors, the flatter the response surface, the denser the contour lines, and the three-dimensional diagram will be flat.

[0045] The response surface curve of the interaction between fermentation temperature and fermentation time on the enzyme activity content of sorghum distiller's grains fermented by bacterial enzymes is shown in Figure 2. Figure 1When the inoculation amount is fixed, the cellulase activity content first increases and then decreases with the increase of fermentation time and fermentation temperature. The higher the fermentation temperature, the lower the enzyme activity content of sorghum wine grains fermented with bacterial enzymes. As the fermentation time increases, the enzyme activity content tends to be stable, which also shows that the enzyme activity of sorghum wine grains fermented with bacterial enzymes is very stable.

[0046] The response surface curve of the interaction between inoculation amount and fermentation time on the enzyme activity content of sorghum distiller's grains fermented by bacterial enzymes is shown in Figure 2. Figure 2 When the fermentation temperature is fixed, the cellulase activity content first increases and then decreases with the increase of inoculation amount and fermentation time. The higher the inoculation amount, the lower the enzyme activity content of sorghum wine grains fermented with bacterial enzymes. As the fermentation time increases, the enzyme activity content tends to be stable, which also shows that the enzyme activity of sorghum wine grains fermented with bacterial enzymes is very stable.

[0047] The response surface curve of the interaction between inoculum size and fermentation temperature on the enzyme activity content of sorghum distiller's grains in bacterial enzyme fermentation is shown in Figure 2. Figure 3 When the fermentation time is fixed, the cellulase activity content first increases and then decreases with the increase of inoculation amount and fermentation temperature. The higher the inoculation amount, the lower the enzyme activity content of sorghum wine grains fermented with bacterial enzymes. As the fermentation temperature increases, the enzyme activity content tends to be stable, which also shows that the enzyme activity of sorghum wine grains fermented with bacterial enzymes is very stable.

[0048] In actual production, enzyme activity is the most important indicator of fermentation of sorghum distiller's grains with bacterial enzymes. The level of enzyme activity greatly determines the competitiveness of the product in the market. According to the results of single factor test and Design-Expert8 response surface method analysis, the optimal process parameters of fermentation of sorghum distiller's grains with bacterial enzymes are: fermentation temperature 30.24℃, fermentation time 6.02d, inoculation amount 36.03%. The enzyme activity content predicted by this process condition is 6.45276.

[0049] 5. Experimental results verification

[0050] In order to verify the reliability of the model prediction, a verification experiment was carried out using the optimal process determined in 2.4. Each sample was made in triplicate, each indicator was measured three times in parallel, and the results were averaged.

[0051] According to actual production, the process parameters were adjusted as follows: fermentation time 6d, fermentation temperature 30℃, inoculation amount 36.0%, and the enzyme activity content was 6.353912±0.67. The results were not significantly different from the predicted values ​​(P>0.05), indicating that the fermentation of sorghum distiller's grains with bacterial enzymes obtained through response surface design is reliable and stable, and can serve as a theoretical support for subsequent practice.

[0052] Example 1

[0053] The biological treatment method of sorghum distiller's grains comprises the following steps:

[0054] (1) Mix 20 g sorghum distiller's grains, 2 g corn flour, 2 g soybean meal, 4 g wheat bran, 0.6 g ammonium sulfate, 0.14 g potassium dihydrogen phosphate, 2 g calcium carbonate, and 1.5 g sodium bicarbonate evenly at a material-water ratio of 1:1.2, sterilize at 121°C for 20 min, and then cool.

[0055] (2) A composite enzyme preparation was prepared by mixing 0.16 g of cellulase H type (enzyme activity ≥ 10,000 U / g), 0.05 g of laccase 10,000 G (enzyme activity ≥ 10,000 U / kg), and 0.05 g of tannin (enzyme activity ≥ 300 U / g). The uniformly mixed composite enzyme preparation was added to the substrate raw material and fermented at 30°C for 1 day.

[0056] (3) At a concentration of 1.6×10 7 CFU / mL of Bacillus subtilis (CICC23830) 1.05mL, concentration 2.2×10 7 CFU / mL of Aspergillus niger (CICC41254) 1.05mL, concentration 5.1×10 7 The composite bacterial agent was prepared by mixing 1.05 mL of white rot fungus (ACCC30942) with a CFU / mL, and the composite bacterial agent was inoculated into the substrate raw material and fermented at 30°C for 4 days.

[0057] (4) After 5 days of fermentation, 0.019 g of lactic acid bacteria (the lactic acid bacteria is Lactobacillus acidophilus Woshun SS-1000, with a concentration of 1.1 × 10 11 CFU / g) were taken out after fermentation at 30℃ for 1 day, and dried in a forced air drying oven at 45℃ and crushed.

[0058] Example 2

[0059] The biological treatment method of sorghum distiller's grains comprises the following steps:

[0060] (1) Mix 620.0 kg of sorghum distiller's grains, 62.0 kg of corn flour, 62.0 kg of soybean meal, 124.0 kg of wheat bran, 1.9 kg of ammonium sulfate, 0.4 kg of potassium dihydrogen phosphate, 6.2 kg of calcium carbonate, and 4.7 kg of sodium bicarbonate evenly at a material-water ratio of 1:1.2, sterilize at 121°C for 20 min, and then cool.

[0061] (2) A composite enzyme preparation was prepared by mixing 5.0 kg of cellulase H type (enzyme activity ≥ 10,000 U / g), 1.5 kg of laccase 10,000 G (enzyme activity ≥ 10,000 U / kg), and 1.5 kg of tannase (enzyme activity ≥ 300 U / g). The composite enzyme preparation was added to the substrate raw material and fermented at 30°C for 1 day. The three enzymes were directly purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0062] (3) The concentration is 1.6×10 7CFU / mL of Bacillus subtilis (CICC23830) 32.0L, concentration of 2.2×10 7 CFU / mL of Aspergillus niger (CICC41254) was 32.0L, and the concentration was 5.1×10 7 The composite bacterial agent was prepared by mixing 32.0 L of white rot fungi (ACCC30942) with a CFU / mL, and then added into the substrate raw material for fermentation at 30°C for 4 days.

[0063] (4) After 5 days of fermentation, 0.6 kg of lactic acid bacteria (the lactic acid bacteria is Lactobacillus acidophilus Woshun SS-1000, with a concentration of 1.1×10 11 CFU / g), mixed well, fermented at 30℃ for 1 day, taken out, dried in a 45℃ forced air drying oven, and crushed.

[0064] (5) The sorghum distiller's grains biological feed was tested for physical and chemical indicators (moisture, crude protein, neutral detergent fiber, acid detergent fiber). The results are shown in Table 5.

[0065] Table 5 Physical and chemical indicators of sorghum distiller's grains biological feed

[0066]

[0067] Aflatoxin B1, Escherichia coli, Salmonella: Not detected.

[0068] As shown in Table 5, after fermenting sorghum distiller's grains with bacterial enzymes, not only most of the original nutrients in the sorghum distiller's grains are retained, but also the cellulase activity content is increased, and the taste and nutritional value are enriched, indicating that it has good feeding quality and has good development prospects and value.

[0069] Application Example 1

[0070] Study on the effect of distiller's grains biological feed on Sansui duck feeding

[0071] This research group conducted a comparative feeding experiment of meat ducks with distiller's grains biological feed at the Animal Husbandry and Veterinary Experimental Field of Guizhou Animal Husbandry and Veterinary Research Institute. The control group was fed with duckling compound feed, the first group was fed with 90% duckling compound feed + 10% distiller's grains biological feed, the second group was fed with 75% duckling compound feed + 25% distiller's grains biological feed, and the third group was fed with 50% duckling compound feed + 50% distiller's grains biological feed. Related experimental examples are as follows:

[0072] Table 6 Effects on the growth and feed conversion efficiency of broiler ducks

[0073]

[0074] Note: a and b with different letters indicate significant difference, p<0.05.

[0075] Table 7 Effects on serum biochemical parameters of broiler ducks

[0076]

[0077] Note: a, b, c with different letters indicate significant difference, p<0.05.

[0078] In this example, as the proportion of distiller's grains biological feed replacing duckling compound feed increases, the average final weight and average daily weight gain of the duckling compound feed replaced by 10% distiller's grains biological feed are significantly higher than those of experimental groups 2 and 3, and the difference with the control group is not significant; while the average daily feed intake, feed-to-weight ratio, total consumption of compound feed, total consumption of metabolic energy, metabolic energy weight gain efficiency, and total consumption of protein have an upward trend; total weight gain, protein efficiency ratio, and survival rate have a downward trend; when the replacement ratio is 25%, each index is basically consistent with the control group, but when the replacement ratio is 50%, each index is significantly lower than the control group, reaching a significant difference level, the results are shown in Table 6. As the proportion of distiller's grains biological feed replacing duckling compound feed increases, serum biochemical indicators among the experimental groups change, especially the content of alkaline phosphatase, lactate dehydrogenase, and serum potassium shows an increasing trend, while the content of conjugated bilirubin shows a decreasing trend, all reaching a significant difference level, the results are shown in Table 7.

[0079] Finally, it should be noted that the above embodiments are only used to illustrate rather than limit the technical solutions of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the present invention can still be modified or replaced by equivalents. Any modification or partial replacement that does not depart from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A biological treatment method for sorghum distiller's grains, characterized in that the steps include: (1) Mix sorghum distiller's grains, corn flour, soybean meal, wheat bran, ammonium sulfate, potassium dihydrogen phosphate, calcium carbonate and sodium bicarbonate evenly at a material-water ratio of 1:1.2, sterilize at 121°C for 20 minutes and then cool; (2) Adding a composite enzyme preparation at 0.8% of the weight of the mixture, and fermenting and culturing at 30-32°C for 1 day, wherein the composite enzyme preparation comprises cellulase H type, laccase 10000G and tannase, and the weight ratios of the three to the mixture are 0.5%, 0.15% and 0.15% respectively; (3) After one day of fermentation, a mixed bacterial agent was inoculated at 9.6% of the weight of the material, and fermented at 30-32°C for 4 days. The composite bacterial agent was Bacillus subtilis (CICC23830), Aspergillus niger (CICC41254) and white rot fungus (ACCC30942), and the dosage ratio was 1:1:1; (4) After 5 days of fermentation, inoculate the material with lactic acid bacteria at 0.06% of the material weight, ferment and culture at 30-32°C for 1 day, take out the material, and dry and crush it in a forced air drying oven at 45°C.

2. The method according to claim 1, characterized in that: In step (1), the weight ratio of the raw materials is: 62.0% sorghum grains, 6.2% corn flour, 6.2% soybean meal, 12.4% wheat bran, 1.9% ammonium sulfate, 0.4% potassium dihydrogen phosphate, 6.2% calcium carbonate, and 4.7% sodium bicarbonate.

3. The method according to claim 1, characterized in that: In step (2), the enzyme activity of the cellulase H type is ≥10000 U / g, the enzyme activity of the laccase 10000G is ≥10000 U / kg, and the enzyme activity of the tannin is ≥300 U / g.

4. The method according to claim 1, characterized in that: In step (3), the concentration of Bacillus subtilis (CICC23830) is 1.6×10 7 CFU / mL, Aspergillus niger (CICC41254) concentration was 2.2×10 7 CFU / mL, white rot fungus (ACCC30942) concentration was 5.1×10 7 CFU / mL.

5. The method according to claim 1, characterized in that: In step (4), the lactic acid bacteria is Lactobacillus acidophilus, and the concentration is 1.1×10 11 CFU / g.

6. The method according to claim 1, characterized in that: In steps (2), (3) and (4), the fermentation temperature is 30°C.

7. Use of sorghum distiller's grains prepared and treated according to any one of claims 1 to 6 as a raw material for compound feed for broiler ducks, characterized in that: Add the processed sorghum grains to the broiler duck feed at 10-25% by weight.

8. The use according to claim 7, characterized in that: Add the processed sorghum distiller's grains to the broiler duck feed at 10% by weight.

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