Application of rhodiola rosea in introduction of Berkshire pigs

Abstract

The invention relates to the technical field of animal feed, in particular to application of rhodiola rosea in introduction of Berkshire pigs. The invention relates to an application of rhodiola rosea in introducing Berkshire pigs to improve the blood oxygen carrying capacity of the Berkshire pigs and / or protect the liver to enhance the immunity and / or reduce the feed-gain ratio to improve the feed conversion rate. The method has the advantages that (1) the feed-to-gain ratio is reduced, and the feed conversion rate is improved; and (2) the blood oxygen carrying capacity of the Berkshire pigs is improved. And (3) liver protection and immunity enhancement. And (4) excessive fat deposition is avoided. And (5) the richness of harmful bacteria is reduced. And (6) increasing the generation of beneficial metabolites.

Description

Technical Field

[0001] The invention relates to the technical field of animal feed, and in particular to application of Rhodiola rosea in the introduction of Berkshire pigs. Background Art

[0002] Altitudes between 2,000 and 4,000 meters are defined as high altitudes. Temperature, humidity, air pressure and oxygen content will gradually decrease with increasing altitude, while the intensity of ultraviolet rays will be the opposite. Qinghai is a typical high-altitude area with a changeable climate and a large temperature difference between day and night. Therefore, compared with local animals that have adapted for a long time, the performance of animals that have adapted for a short period of time or moved to the plateau is restricted by the plateau environment, which in turn affects the development of animal husbandry in the plateau area. Ordinary pigs introduced to the plateau have problems such as loss of appetite, lethargy, and inability to fully exert production performance.

[0003] Berkshire pigs are a lean meat type pig breed, produced in Berkshire, England and its surrounding areas. Modern Berkshire pigs have a long and wide body, a short nose and concave face, upright or slightly forward ears, a deep chest and wide hips, black fur, and the "six white" characteristics (white nose tip, tail brush and limb ends). Adult boars weigh about 400 kg, and sows weigh about 370 kg. They have excellent meat quality, fast growth rate, high feed conversion rate, thin back fat and high intramuscular fat content, and strong resistance to roughage and adversity. At present, Berkshire pigs are widely used as terminal sires in the international pig farming industry to produce high-quality hybrid pigs.

[0004] Rhodiola rosea is a traditional Chinese herbal medicine for anti-altitude sickness, of which Rhodiola polysaccharide (RDP) is its main active ingredient, which has multiple biological functions such as anti-inflammatory, anti-oxidation, immunity enhancement, protection of reproductive cells, liver protection and anti-hypoxia. The present invention monitors the effect of RDP on the growth performance, physiological and biochemical indicators, fecal microorganisms and metabolites of the introduced Berkshire pigs, in order to provide basic data for RDP as a new feed additive to improve the plateau adaptability of pigs. Summary of the invention

[0005] The purpose of the present invention is to provide an application of Rhodiola rosea for effectively treating plateau disease of introduced Berkshire pigs, reducing feed-to-weight ratio, improving feed conversion rate, improving blood oxygen carrying capacity of Berkshire pigs, protecting liver and enhancing immunity.

[0006] In a first aspect, the present invention provides a use of Rhodiola rosea in the introduction of Berkshire pigs.

[0007] The invention discloses an application of Rhodiola rosea in the introduction of Berkshire pigs to improve the blood oxygen carrying capacity of Berkshire pigs and / or protect the liver to enhance immunity and / or reduce the feed-to-weight ratio to improve the feed conversion rate.

[0008] Furthermore, the Rhodiola rosea includes but is not limited to the Chinese medicine Rhodiola rosea, Rhodiola rosea decoction pieces, Rhodiola rosea extract, Rhodiola rosea extract or Rhodiola rosea polysaccharide.

[0009] Furthermore, the Rhodiola rosea is Rhodiola rosea polysaccharide.

[0010] Furthermore, the Rhodiola rosea is a feed additive or a pharmaceutical preparation.

[0011] Furthermore, the amount of Rhodiola rosea added is 150-300 mg / kg based on the Rhodiola rosea polysaccharide content.

[0012] In a second aspect, the present invention provides an animal feed.

[0013] An animal feed, the feed comprising any one of the Chinese medicine Rhodiola rosea, Rhodiola rosea slices, Rhodiola rosea extract, Rhodiola rosea extract, or Rhodiola rosea polysaccharide;

[0014] The feed is used to improve the blood oxygen carrying capacity of Berkshire pigs and / or protect the liver to enhance immunity and / or reduce the feed-to-weight ratio to improve the feed conversion rate.

[0015] The application of the Rhodiola rosea provided by the present invention in the Berkshire pig breed is to reduce the average daily feed intake and feed-to-weight ratio by adding Rhodiola rosea to the basic diet of the Berkshire pig breed, without affecting the initial and final body weights and average daily weight gain of the Berkshire pig breeds. The feed-to-weight ratio of the Berkshire pig breed can be effectively reduced, and the feed conversion rate can be improved.

[0016] The application of Rhodiola rosea provided by the present invention in the introduction of Berkshire pigs is to add Rhodiola rosea to the basic diet of the introduction of Berkshire pigs, thereby increasing the number of red blood cells, hemoglobin concentration, and average hemoglobin concentration in the blood, thereby improving the oxygen carrying capacity of Berkshire pigs; reducing the levels of aspartate aminotransferase and creatine kinase in serum, thereby protecting the liver and enhancing immunity. In addition, the addition of Rhodiola rosea polysaccharide can regulate the structure of intestinal flora, reduce the ratio of Firmicutes / Bacteroidetes, avoid excessive fat deposition, affect the production of metabolites in the blood, and protect the health of Berkshire pigs.

[0017] The invention provides an application of Rhodiola rosea in the introduction of Berkshire pigs, wherein the Rhodiola rosea can be any one of the Chinese medicine Rhodiola rosea, Rhodiola rosea slices, Rhodiola rosea extract, Rhodiola rosea extract or Rhodiola rosea polysaccharide, preferably Rhodiola rosea polysaccharide (RDP).

[0018] Compared with the prior art, the application of Rhodiola rosea provided by the present invention in the introduction of Berkshire pigs has the following advantages:

[0019] (1) Reduce feed-to-weight ratio and improve feed conversion rate.

[0020] (2) Improve the blood oxygen carrying capacity of Berkshire pigs.

[0021] (3) Protect the liver and enhance immunity.

[0022] (4) Avoid excessive fat deposition.

[0023] (5) Reduce the relative abundance of harmful bacterial genera.

[0024] (6) Increase the production of beneficial metabolites and have anti-inflammatory effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is the ASV-based Veen diagram of feces provided in Example 2 of the present invention.

[0026] Figure 2 This is the principal coordinate analysis (PCoA) result of fecal microorganisms provided in Example 2 of the present invention.

[0027] Figure 3 This is the microbial community structure analysis result provided in Example 2 of the present invention.

[0028] Figure 4 This is the fecal flora difference comparison and function prediction result provided in Example 2 of the present invention.

[0029] Figure 5 This is the difference in metabolomics results between the experimental group and the control group on the 14th day provided in Example 3 of the present invention.

[0030] Figure 6 This is the difference in metabolomics results between the test group and the control group on day 30 provided in Example 3 of the present invention.

[0031] Figure 1 The figure is a Venn diagram; circles of different colors represent different groups, and the overlapping part represents the number of ASVs shared between the groups. A is the fecal microorganisms of the CK group on days 0, 14, and 30; B is the fecal microorganisms of the LH group on days 0, 14, and 30; C is the fecal microorganisms of the CK group and the LH group on day 14; D is the fecal microorganisms of the CK group and the LH group on day 30

[0032] Figure 2 A is the CK group on days 0, 14, and 30; B is the LH group on days 0, 14, and 30; C is CK vs LH on day 14; D is CK vs LH on day 30

[0033] Figure 3 A is the top 15 phyla at the phylum level on the 14th day; B is the top 15 genera at the genus level on the 14th day; C is the top 15 phyla at the phylum level on the 30th day; D is the top 15 genera at the genus level on the 30th day

[0034] Figure 4 A is the LEfSe analysis diagram; the letters in front of ASVs represent the classification level (p: phylum; c: class; o: order; f: family; g: genus), and the bar graph represents the difference in fecal microbial communities at different time stages. Different colors represent different feeding times and groups of Berkshire pigs; B is the potential function of Berkshire pig fecal microbial communities predicted based on PICRUSt

[0035] Figure 5 and Figure 6 A is a heat map of differential metabolites; B is a differential volcano map, where each point represents a specific metabolite, and the size of the point represents the size of the VIP value. Red represents upregulation, and blue represents downregulation; C is a KEGG pathway bar chart, where the horizontal axis is the P value of the differential metabolites annotated to the pathway, and the vertical axis is the name of the secondary classification pathway enriched; D is a topological analysis diagram of the enriched pathway, where the horizontal axis represents the size of the impact value, and the larger the bubble, the greater the impact of the pathway; the Y axis is log10(P), which represents the credibility of the P value, and the redder the color, the greater the credibility. DETAILED DESCRIPTION

[0036] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention is further described in detail in the following examples. The following examples are only used to illustrate the invention but are not used to limit the scope of the present invention.

[0037] In a first aspect, the present invention provides a use of Rhodiola rosea in the introduction of Berkshire pigs.

[0038] The invention discloses an application of Rhodiola rosea in the introduction of Berkshire pigs to improve the blood oxygen carrying capacity of Berkshire pigs and / or protect the liver to enhance immunity and / or reduce the feed-to-weight ratio to improve the feed conversion rate.

[0039] Furthermore, the Rhodiola rosea includes but is not limited to the Chinese medicine Rhodiola rosea, Rhodiola rosea decoction pieces, Rhodiola rosea extract, Rhodiola rosea extract or Rhodiola rosea polysaccharide.

[0040] Furthermore, the Rhodiola rosea is Rhodiola rosea polysaccharide.

[0041] Furthermore, the Rhodiola rosea is a feed additive or a pharmaceutical preparation.

[0042] Furthermore, the amount of Rhodiola rosea added is 150-300 mg / kg based on the Rhodiola rosea polysaccharide content.

[0043] In a second aspect, the present invention provides an animal feed.

[0044] An animal feed, the feed comprising any one of the Chinese medicine Rhodiola rosea, Rhodiola rosea slices, Rhodiola rosea extract, Rhodiola rosea extract, or Rhodiola rosea polysaccharide;

[0045] The feed is used to improve the blood oxygen carrying capacity of Berkshire pigs and / or protect the liver to enhance immunity and / or reduce the feed-to-weight ratio to improve the feed conversion rate.

[0046] Example 1

[0047] Experiment on the introduction of Berkshire pigs

[0048] Berkshire pigs were introduced from Hubei Wuhan Dawu Hanwu Animal Husbandry Co., Ltd. (environmental conditions: 41m above sea level, temperature 15-26℃, humidity 94%, oxygen content about 20.88%) to Qinghai Huzhu Bamei Pig Original Breeding Farm (environmental conditions: 2600m above sea level, temperature -1-7℃, humidity 89%, oxygen content about 16.70%). For this experiment, 16 healthy Berkshire pigs of similar weight were selected and randomly divided into two groups. The control group was fed with a basic diet, and the experimental group was fed with a basic diet + RDP (200mg / kg). The composition of the basic diet is shown in Table 2. The experimental grouping is shown in Table 1, and the composition and nutritional components of the basic diet are shown in Table 2.

[0049] Table 1 Test groups

[0050]

[0051] Table 2 Basic feed composition and nutritional level (%)

[0052]

[0053] Note: 1) Premix nutrient composition: Premix provides per kg feed: Vitamin A 250 KIU, Vitamin D 368 KIU, Vitamin E 638 IU, Vitamin K 360 mg, Vitamin B1 29 mg, Vitamin B2 154 mg, Vitamin B6 118 mg, Niacin 713 mg, Pantothenic acid 276 mg, Copper 2125 mg, Iron 2550 mg, Manganese 650 mg, Zinc 2125 mg, Moisture ≤ 10%. 2) Nutritional level is calculated value.

[0054] Example 2

[0055] Effects of Rhodiola rosea polysaccharide on growth performance of introduced Berkshire pigs

[0056] Growth performance index determination: Determine the initial and final weights, calculate daily weight gain and feed-to-weight ratio. Average daily weight gain (ADG) = (final weight - initial weight) / total number of days. Collect the remaining feed every day and calculate the average daily feed intake (ADFI) = (total feed intake - remaining feed) / total number of days, feed-to-weight ratio (F / G) = average daily feed intake / average daily weight gain.

[0057] The results of the growth performance test of Berkshire pigs are shown in Table 3. The feed-to-weight ratio of the LH group was significantly lower than that of the CK group (P < 0.05), the average daily weight gain and average feed intake of the LH group were higher than those of the CK group (P > 0.05), and there was no significant difference in the final body weight between the two groups (P > 0.05).

[0058] Table 3 Effects of Rhodiola rosea polysaccharide on growth performance

[0059]

[0060] Note: Different lowercase letters indicate significant differences (P<0.05), no letters or the same letters indicate no significant differences (P>0.05).

[0061] The above results show that adding Rhodiola rosea polysaccharide to the diet can improve the feed-to-weight ratio of Berkshire pigs and increase feed conversion rate.

[0062] Example 3

[0063] Effects of Rhodiola rosea polysaccharide on blood physiological parameters of introduced Berkshire pigs

[0064] Blood biochemical indexes were measured: white blood cell count WBC, neutrophil NE, lymphocyte LY, monocyte MO, eosinophil EO, red blood cell count RBC, hemoglobin HGB, hematocrit HCT, mean red blood cell volume MCV, mean hemoglobin MCH, mean hemoglobin concentration MCHC, platelets PLT, mean platelet volume MPV, platelet volume distribution width PDW, and large platelet ratio P-LCR.

[0065] As shown in Table 4, WBC, NE, MO, and EO indices decreased and then slowly increased over time, and the indices of the LH group were higher than those of the CK group on the 14th and 30th days. LY showed an increasing and decreasing trend over time.

[0066] Table 4 Effects of Rhodiola rosea polysaccharide on leukocyte-related indices in Berkshire pigs

[0067]

[0068]

[0069] Note: Different lowercase letters indicate significant differences (P<0.05), and no letters indicate no significant differences (P>0.05).

[0070] As shown in the table, the four indicators of RBC, HGB, HCT, and MCHC showed a slow upward trend over time. Compared with the CK group, the four indicators of the LH group increased by 5.92%, 2.67%, 2.08%, and 0.70% on the 14th day, and increased by 4.13%, 1.99%, 1.76%, and 0.21% on the 30th day. MCV showed a slow downward trend over time, and the LH group was lower than the CK group.

[0071] Table 5 Effects of Rhodiola rosea polysaccharide on related parameters of Berkshire pig erythrocytes

[0072]

[0073] Note: Different lowercase letters indicate significant differences (P<0.05), and no letters indicate no significant differences (P>0.05).

[0074] As shown in Table 6, the five indices of PLT, PCT, MPV, PDW, and P-LCR gradually decreased over time. The PLT index was higher than that of the CK group on the 14th and 30th days, and the MPV, PDW, and P-LCR indexes were lower than those of the CK group on the 14th and 30th days.

[0075] Table 6 Effects of Rhodiola rosea polysaccharide on platelet-related indices in Berkshire pigs

[0076]

[0077] Note: Different lowercase letters indicate significant differences (P<0.05), and no letters indicate no significant differences (P>0.05).

[0078] The above results show that adding Rhodiola rosea polysaccharide to the diet can improve the oxygen carrying capacity of Berkshire pigs by increasing the number of red blood cells, hemoglobin concentration, and average hemoglobin concentration in the blood.

[0079] Example 4

[0080] Effects of Rhodiola rosea polysaccharide on blood biochemical parameters of introduced Berkshire pigs

[0081] Blood physiological indexes determination: alanine aminotransferase ALT, aspartate aminotransferase AST, creatine kinase CK, immunoglobulin G IgG, immune protein M IgM, C-reactive protein CRP.

[0082] The blood biochemical indices of Berkshire pigs are shown in Table 7: ALT, AST, and CK showed a downward trend over time, and the three indices were lower than those in the CK group on the 14th and 30th days. IgG and IgM showed a slow upward trend over time, AST and CK in the LH group were lower than those in the CK group, and ALT was higher than that in the CK group; there was little difference between the CK group and the LH group, and IgM and C-reactive protein showed a downward and upward trend over time.

[0083] Table 7 Effects of Rhodiola rosea polysaccharide on biochemical parameters of Berkshire pigs

[0084]

[0085] Note: Different lowercase letters indicate significant differences (P<0.05), and no letters indicate no significant differences (P>0.05).

[0086] The above results show that adding Rhodiola rosea polysaccharide to the diet can reduce the levels of alanine aminotransferase, aspartate aminotransferase, and creatine kinase in serum, increase the level of immune protein M, and thus protect the liver and enhance the immunity of introduced Berkshire pigs.

[0087] Example 5

[0088] Effects of Rhodiola rosea polysaccharide on fecal microorganisms of introduced Berkshire pigs

[0089] Fecal microbial index determination: Genomic DNA was extracted from fecal samples using the MagPure Soil DNA LQ Kit (Magan) according to the instructions. DNA was used as a template. After amplification, the DNA was sequenced, quality controlled and spliced ​​by Shanghai Ouyi Biomedical Technology Co., Ltd. using the IlluminaNovaSeq 6000 sequencing platform to construct a library. 16S was aligned using the Silva database. Species annotation was performed using the q2-feature-classifier software. Alpha diversity and beta diversity analysis were performed using the QIIME2 software. PCOA analysis was performed using the distance matrix calculated using R to evaluate beta diversity in feces. Based on the R package, the Kruskal Wallis statistical algorithm and LEfSe were used for differential analysis.

[0090] (1) Cluster analysis of fecal microbial ASV

[0091] To investigate the effect of RDP addition on the diversity of fecal microorganisms in the Berkshire pigs, 16S sequencing was performed on feces from three time periods to study the fecal microbial composition based on 100% sequence similarity. Figure 1 As shown: the number of ASVs unique to each group of CK_0, CK_14, and CK_30 were 835, 477, and 664, respectively; the number of ASVs unique to LH_0, LH_14, and LH_30 were 860, 817, and 713, respectively; the number of ASVs unique to the CK group and LH group on the 14th day were 575 and 1154, respectively; the number of ASVs unique to the CK group and LH group on the 30th day were 905 and 1002, respectively.

[0092] (2) Analysis of fecal microbial alpha diversity

[0093] As shown in Table 8, the diversity of microorganisms in Berkshire pig feces showed an obvious trend of change over time. The results of Chao1 index and observed_species index showed that the diversity of fecal microorganisms decreased on the 14th day and increased on the 30th day. In addition, the diversity of the LH group was higher than that of the CK group on the 14th and 30th days (P>0.05).

[0094] Table 8 Analysis of fecal microbial Alpha diversity

[0095]

[0096] Note: (A) Chao1 index is an index for predicting species richness; (B) Shannon index is a comprehensive index for measuring community diversity, which reflects the richness and uniformity of microbial communities; (C) Simpson index is one of the indices for measuring microbial diversity; (D) Observed_specics index is the number of microbial species observed in a specific area or sample; Means with different lowercase letters indicate significant differences (P < 0.05), and means with the same lowercase letters indicate no significant differences (P > 0.05).

[0097] (3) Analysis of fecal microbial Beta diversity

[0098] The differences or similarities of microbial diversity in different time periods were comprehensively analyzed based on Bray-Curtis principal coordinates (PCoA). Figure 2 As shown in the figure, although the PCoA graphs of the CK group and the LH group were crossed, the multivariate analysis of variance in the three time periods of the LH group (P=0.026) showed that the microbial communities were different; there was no difference between the CK group and the LH group on the 14th day; there was a significant difference between the CK group and the LH group on the 30th day (P=0.014), indicating that there were significant differences in the fecal microbial communities between the two groups (P<0.05).

[0099] (4) Analysis of microbial community structure

[0100] To further analyze the composition of fecal microorganisms, we found that the effect of RDP on the fecal microbial composition of Berkshire pigs was as follows: Figure 4 As shown. At the phylum level, the dominant phyla in both the control and experimental groups were Firmicutes and Bacteroidota. The Firmicutes in the CK group increased and then leveled off over time, while the Firmicutes in the LH group increased and then decreased over time, and the Bacteroidetes had the opposite trend. The relative abundance values ​​of Firmicutes were all above 49%, and the relative abundance values ​​of Bacteroidetes were above 33%. At 30 days, compared with the CK group, RDP in the LH group significantly increased the relative abundance of Bacteroidetes and reduced the ratio of Firmicutes to Bacteroidetes.

[0101] At the genus level, the dominant genera on the 14th day were Muribaculaceae and Streptococcus, and the dominant genera on the 30th day were Muribaculaceae and Rikenellaceae_RC9_gut_group. RDP significantly increased the relative abundance of Rikenellaceae_RC9_gut_group and NK4A214_group, and decreased the relative abundance of Prevotella and Streptococcus.

[0102] The above results show that adding Rhodiola rosea polysaccharide to the diet can regulate the intestinal flora structure, reduce the F / B ratio, avoid excessive fat deposition, affect the production of metabolites in the blood, and protect the health of the introduced Berkshire pigs. And over time, Rhodiola rosea polysaccharide reduced the relative abundance of harmful bacteria.

[0103] Example 6

[0104] Analysis of serum metabolome in introduced Berkshire pigs

[0105] Serum samples were processed and tested by LC-MS. The analysis was performed using Shimadzu LC-30AD ultra-high liquid chromatography and ThermoFisher QE high-resolution mass spectrometer. The OPLS-DA model was performed using the OuYi Cloud platform, and differential metabolites were screened based on VIP>1 and P<0.05. The KEGG database was used for pathway enrichment analysis, and Spearman correlation analysis generated correlation heat maps.

[0106] On the 14th day, a total of 59 differential metabolites were identified between the CK group and the LH group. Figure 5 A), of which 28 were significantly upregulated and 31 were significantly downregulated (VIP≥1.0, P<0.05). The annotated metabolites were mainly enriched in the biosynthesis pathways of valine, leucine, and isoleucine, the biosynthesis pathways of pantothenate and coenzyme A, and the metabolic pathways of ascorbic acid and aldonic acid ( Figure 5 C) In addition, if Figure 5As shown in Figure D, pathway enrichment and topological analysis were performed, and it was found that the metabolites on the 14th day were mainly concentrated in three pathways: glycerophospholipid metabolism, fat metabolism, and the interconversion of pentose and glucuronic acid. Among them, 31 metabolites were higher in the CK group, including 11 glycerophospholipids (e.g., phosphatidylserine, phosphatidic acid); 5 benzene and derivative substitutions (e.g., hippuric acid, m-trifluoromethylhippuric acid); 3 carboxylic acids and derivatives (e.g., L-valine, L-arginine). 28 metabolites were higher in the LH group, including 8 glycerophospholipids (e.g., phosphatidylcholine); 5 organic oxygen compounds (e.g., 2-O-methyl-L-fucose, stearoylcarnitine); 5 fatty acyl groups (e.g., L-palmitoylcarnitine, fatty acylcarnitine); 2 flavonoids (cyanidin-3-arabinoside, apigenin 7-(6"-malonylneohesperidoside)).

[0107] like Figure 6 As shown in the figure, at 30 days of feeding, a total of 28 differential metabolites were identified between the CK group and the LH group, of which 7 were significantly upregulated and 21 were significantly downregulated. The differential metabolites were mainly enriched in the linoleic acid metabolic pathway, pantothenate and coenzyme A biosynthesis pathway, inositol phosphate metabolic pathway, tryptophan metabolism and other metabolic pathways ( Figure 6 C). At the same time, topological analysis was performed on differential metabolites such as Beta-Alanine and Uracil ( Figure 6 D), and found that these metabolites were mainly enriched in β-alanine metabolism, pantothenate and CoA biosynthesis, and pyrimidine metabolism. Among them, 21 metabolites were higher in the CK group, including 7 glycerophospholipids (including phosphatidylserine); 3 carboxylic acids and their derivatives (L-arginine, β-alanine, L-pyroglutamic acid); 2 fatty acyl (((12z,15z)-10-hydroxyoctadecane-12,15-dienoylcarnitine, nonadecanoic acid)). 7 metabolites were higher in LH, including 1 indole and its derivative L-tryptophan; 1 fatty acyl dasyphyllanthrin E; and 1 organic oxygen compound 2-O-galloylgalactobaric acid.

[0108] The above results show that the addition of Rhodiola rosea polysaccharide has an effect on serum metabolites. On the 14th day, most of the metabolites that promote fat deposition were found to be more abundant in the CK group, and the metabolites of L-palmitoylcarnitine, cyanidin-3-arabinoside, and apigenin 7-(6”-malonylneohesperidoside related to anti-inflammatory and antibacterial were more abundant in the LH group. On the 30th day, glycerophospholipid metabolites were more abundant in the CK group, and the metabolite sigeranol E related to antioxidants was found to be more abundant in the LH group.

[0109] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various changes can be made to the technical scheme of the present invention. These simple variations all belong to the protection scope of the present invention.

[0110] It should also be noted that the various specific technical features and steps described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0111] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. An application of Rhodiola rosea in the introduction of Berkshire pigs to improve the blood oxygen carrying capacity of Berkshire pigs and / or protect the liver to enhance immunity and / or reduce the feed-to-weight ratio to improve feed conversion rate.

2. The use according to claim 1, characterized in that: The Rhodiola rosea includes but is not limited to the Chinese medicine Rhodiola rosea, Rhodiola rosea decoction pieces, Rhodiola rosea extract, Rhodiola rosea extract or Rhodiola rosea polysaccharide.

3. The use according to claim 1, characterized in that: The Rhodiola rosea is Rhodiola rosea polysaccharide.

4. The use according to claim 1, characterized in that: The rhodiola rosea is a feed additive or a pharmaceutical preparation.

5. The use according to claim 4, characterized in that: The amount of Rhodiola rosea added is 150-300 mg / kg based on the Rhodiola rosea polysaccharide content.

6. An animal feed, characterized in that: The feed contains any one of the Chinese medicine Rhodiola rosea, Rhodiola rosea slices, Rhodiola rosea extract, Rhodiola rosea extract, or Rhodiola rosea polysaccharide; The feed is used to improve the blood oxygen carrying capacity of Berkshire pigs and / or protect the liver to enhance immunity and / or reduce the feed-to-weight ratio to improve the feed conversion rate.

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